FastQCFastQC Report
Sat 18 Jun 2016
SRR1930914_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1930914_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2832989
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35470.1252034511958924No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30410.10734245703036616No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA28700.051.6256071
GTATCAA57300.042.2676471
CAACGCA69000.034.1092645
ATCAACG69700.033.786983
TCAACGC69450.033.771284
AACGCAG72450.032.6809166
TATCAAC73850.032.145482
ACGCAGA83200.028.1161377
CGCAGAG87250.026.5935028
GTACATG72400.023.3509181
GCAGAGT97800.023.0940539
TACATGG73900.022.4223672
ACATGGG74300.021.6626363
AGAGTAC89650.020.72113610-11
CGTATAC2902.4556357E-919.6464313
CAGAGTA93750.019.30880410-11
CATGGGG53950.019.2728274
TGGTATC16000.018.991552
GAGTACT60000.018.86119512-13
GTGGTAT17900.018.8363971
Produced by FastQC (version 0.11.5)