##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1930914_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2832989 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.25011710246669 31.0 31.0 33.0 30.0 34.0 2 31.373357609224744 31.0 31.0 34.0 30.0 34.0 3 31.453489935894563 31.0 31.0 34.0 30.0 34.0 4 35.325271294734996 37.0 35.0 37.0 33.0 37.0 5 35.1077918057571 37.0 35.0 37.0 32.0 37.0 6 35.14187453604656 37.0 35.0 37.0 32.0 37.0 7 35.01975404775663 36.0 35.0 37.0 32.0 37.0 8 34.994972094843995 36.0 35.0 37.0 32.0 37.0 9 36.50453460991201 38.0 35.0 39.0 32.0 39.0 10-11 36.428336290751574 38.0 35.0 39.0 32.0 39.0 12-13 36.33214442414001 38.0 35.0 39.0 32.0 39.0 14-15 37.35648814732426 39.0 36.0 41.0 32.0 41.0 16-17 37.324130450206475 39.0 36.0 41.0 32.0 41.0 18-19 37.306742631192705 39.0 36.0 40.0 32.0 41.0 20-21 37.268058047525074 39.0 36.0 40.0 31.0 41.0 22-23 37.14904152469353 39.0 36.0 40.0 31.0 41.0 24-25 37.036039144521915 39.0 36.0 40.0 31.0 41.0 26-27 36.77077461296179 39.0 35.5 40.0 30.5 41.0 28-29 36.58637237913737 38.0 35.0 40.0 30.0 41.0 30-31 36.3562322338703 38.0 35.0 40.0 30.0 41.0 32-33 36.12722357905378 38.0 35.0 40.0 30.0 41.0 34-35 36.33382180446165 38.0 35.0 40.0 30.0 41.0 36-37 36.39595988547785 38.0 35.0 40.0 30.0 41.0 38-39 36.36420455568306 38.0 35.0 40.0 30.0 41.0 40-41 36.271593536014436 38.0 35.0 40.0 30.0 41.0 42-43 36.13869732639273 38.0 35.0 40.0 30.0 41.0 44-45 35.97622722855613 38.0 35.0 40.0 29.0 41.0 46-47 35.77330409683906 38.0 34.5 40.0 28.5 41.0 48-49 35.58983268201889 38.0 34.0 40.0 28.0 41.0 50-51 35.331727020472016 38.0 34.0 40.0 27.0 41.0 52-53 35.128446845363676 38.0 34.0 40.0 27.0 41.0 54-55 34.96634649834503 38.0 33.0 40.0 26.0 41.0 56-57 34.722502452356856 38.0 33.0 40.0 26.0 41.0 58-59 34.48511289666144 37.0 33.0 40.0 26.0 41.0 60-61 34.18727693612647 37.0 33.0 40.0 25.5 41.0 62-63 33.88825265470498 36.5 32.0 39.5 25.0 41.0 64-65 33.59352418946914 36.0 32.0 39.0 24.5 41.0 66-67 33.2714705210645 36.0 31.5 39.0 23.5 40.0 68-69 32.913040078870765 35.0 31.0 39.0 23.0 40.0 70-71 32.51972969185549 35.0 31.0 38.0 22.0 40.0 72-73 32.10889046868873 35.0 31.0 37.5 22.0 40.0 74-75 31.586260836169856 34.5 30.0 37.0 20.0 39.0 76-77 30.20263968550531 33.5 28.5 35.5 19.5 38.5 78-79 30.977587452686898 34.0 30.0 36.0 20.0 39.0 80-81 30.978351486715972 34.0 30.0 36.0 20.0 38.5 82-83 30.694275021893837 34.0 30.0 35.5 20.0 37.0 84-85 30.362445283056164 34.0 30.0 35.0 18.5 37.0 86-87 30.009242887988623 34.0 29.5 35.0 18.0 36.0 88-89 29.65308831061469 34.0 29.0 35.0 14.0 36.0 90-91 29.307205746298344 34.0 29.0 35.0 8.5 36.0 92-93 28.94508344367027 34.0 29.0 35.0 4.5 35.0 94-95 28.51850060130837 33.0 29.0 35.0 2.0 35.0 96-97 28.051428897182447 33.0 28.5 35.0 2.0 35.0 98-99 27.55757523237824 33.0 27.0 35.0 2.0 35.0 100-101 26.345035579029783 32.0 25.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.5144516418715597 1101 2 0.588367102377493 1101 3 0.514718264724106 1101 4 0.4418678775835616 1101 5 0.5259952757280715 1101 6 0.48164967754620847 1101 7 0.5599432107791174 1101 8 0.5664126205740629 1101 9 0.6589474814088092 1101 10-11 0.6583576517701601 1101 12-13 0.6869637848567365 1101 14-15 0.853791709167659 1101 16-17 0.7527894322869599 1101 18-19 0.8043260749996719 1101 20-21 0.6525805058624812 1101 22-23 0.8077937975102358 1101 24-25 0.7756594771163847 1101 26-27 0.7501476573870463 1101 28-29 0.7756176217759503 1101 30-31 0.8130758951005319 1101 32-33 0.886136970441072 1101 34-35 0.7120210728879997 1101 36-37 0.8457750542533802 1101 38-39 1.0166374078182443 1101 40-41 0.826831991076368 1101 42-43 0.8309561797238771 1101 44-45 0.8580203246541984 1101 46-47 0.8652391842053078 1101 48-49 0.9776551787228769 1101 50-51 0.9004361692804892 1101 52-53 0.9378693827415319 1101 54-55 0.9764265954468954 1101 56-57 0.9527466218215324 1101 58-59 0.9042662745146757 1101 60-61 0.9217587579694211 1101 62-63 0.8851364228527103 1101 64-65 0.9267437296322569 1101 66-67 0.9266768108146834 1101 68-69 0.8403584193421096 1101 70-71 0.7966743850205091 1101 72-73 0.8097680886809044 1101 74-75 0.8133308639165051 1101 76-77 0.8913187343631499 1101 78-79 0.8681003164075243 1101 80-81 0.8353026628944207 1101 82-83 0.9121291561485876 1101 84-85 1.1349306874979987 1101 86-87 0.9927517431883182 1101 88-89 0.9051052744649581 1101 90-91 0.818687434463147 1101 92-93 0.8843093558358 1101 94-95 0.9688966156009151 1101 96-97 0.9525834003326032 1101 98-99 0.9925141463525975 1101 100-101 1.1703370898743124 1102 1 0.53580711522957 1102 2 0.5185170729878799 1102 3 0.534546201076278 1102 4 0.3068532144029916 1102 5 0.5055816931635917 1102 6 0.40031838454328295 1102 7 0.47300665665139974 1102 8 0.5157964100676296 1102 9 0.7022453167881153 1102 10-11 0.6255470062694357 1102 12-13 0.6440854461085763 1102 14-15 0.8364710388947287 1102 16-17 0.7256352143014198 1102 18-19 0.8712239279088436 1102 20-21 0.8186459392045364 1102 22-23 0.7972589991690668 1102 24-25 0.8168642908684589 1102 26-27 0.9074248960916975 1102 28-29 1.0076490133165308 1102 30-31 0.9138111098209123 1102 32-33 0.9901187660492994 1102 34-35 0.8555629030499148 1102 36-37 0.8786965037915806 1102 38-39 0.931425658027095 1102 40-41 0.7906191194649281 1102 42-43 0.7726927399057288 1102 44-45 0.8438183757681728 1102 46-47 0.8830961979766414 1102 48-49 0.8954111360494323 1102 50-51 0.8769583637471072 1102 52-53 0.8172250205548792 1102 54-55 0.8669166836247726 1102 56-57 0.7168486663536839 1102 58-59 0.8547181200513663 1102 60-61 0.7619222396694667 1102 62-63 0.6710052484925555 1102 64-65 0.6512451905287122 1102 66-67 0.6575973947033944 1102 68-69 0.7358664281811258 1102 70-71 0.701143289183257 1102 72-73 0.7181900765392228 1102 74-75 0.9103906827355246 1102 76-77 1.0920732296637468 1102 78-79 1.123108579836046 1102 80-81 0.9446113541327037 1102 82-83 1.0471815446589439 1102 84-85 1.2537585863507417 1102 86-87 1.1731266539012672 1102 88-89 1.1979504058869068 1102 90-91 1.0772088974649883 1102 92-93 1.0944698827615227 1102 94-95 1.1971109153532815 1102 96-97 1.1614826700876861 1102 98-99 1.2042546756005699 1102 100-101 1.3940290374817366 1103 1 0.4105012145512319 1103 2 0.48447819166619865 1103 3 0.4546087493575932 1103 4 0.21276377161516535 1103 5 0.27936074217748086 1103 6 0.4009710398602948 1103 7 0.4392399483896696 1103 8 0.37462584413919586 1103 9 0.451887767631483 1103 10-11 0.46594001968779253 1103 12-13 0.5151389787412839 1103 14-15 0.640976298275092 1103 16-17 0.580756598445916 1103 18-19 0.5355666697309687 1103 20-21 0.6419609331512035 1103 22-23 0.6586354914586678 1103 24-25 0.5859707556730669 1103 26-27 0.6070603633999667 1103 28-29 0.7441463454045092 1103 30-31 0.7575811412821025 1103 32-33 0.6943153722192577 1103 34-35 0.633311982561743 1103 36-37 0.6077124368921076 1103 38-39 0.6614264543853068 1103 40-41 0.7599971300993857 1103 42-43 0.7532171893909094 1103 44-45 0.8414664309124547 1103 46-47 0.7010207092663876 1103 48-49 0.685524992872736 1103 50-51 0.6182390533597157 1103 52-53 0.5847758987162095 1103 54-55 0.7705390270392982 1103 56-57 0.8199600300493586 1103 58-59 0.7780715542425511 1103 60-61 0.6639614431186018 1103 62-63 0.6788620334925284 1103 64-65 0.7011287441411511 1103 66-67 0.47683892118880067 1103 68-69 0.7424786654627269 1103 70-71 0.6468237067124107 1103 72-73 0.6213149480830609 1103 74-75 0.7608622757644525 1103 76-77 0.8326156918532526 1103 78-79 0.896395981258717 1103 80-81 0.7703780337210588 1103 82-83 0.78809519265943 1103 84-85 0.9146297775939836 1103 86-87 0.8009978696736866 1103 88-89 0.8218683359648651 1103 90-91 0.9030124268215438 1103 92-93 0.83569977793681 1103 94-95 0.947759734486219 1103 96-97 0.7918644303260685 1103 98-99 0.9272636484631249 1103 100-101 1.1670816196368392 1104 1 0.39232824623496043 1104 2 0.5318854720311457 1104 3 0.3855500423580871 1104 4 0.17098175566191287 1104 5 0.34469141586635743 1104 6 0.3739067016671882 1104 7 0.3624517732154757 1104 8 0.45778372650910626 1104 9 0.5117098941180203 1104 10-11 0.5546712311542308 1104 12-13 0.5231042420308256 1104 14-15 0.6741758661469106 1104 16-17 0.5947637066667042 1104 18-19 0.6646645774624389 1104 20-21 0.6293303852819392 1104 22-23 0.5283486163692501 1104 24-25 0.49680413301783233 1104 26-27 0.6855057472672712 1104 28-29 0.5551435221217247 1104 30-31 0.5924651042826383 1104 32-33 0.6439781934399988 1104 34-35 0.6183656466750946 1104 36-37 0.6850170555227422 1104 38-39 0.728659464866908 1104 40-41 0.6549532886780014 1104 42-43 0.5118221738855269 1104 44-45 0.4493651774706464 1104 46-47 0.5955316844721636 1104 48-49 0.6760181401012275 1104 50-51 0.5722781660322269 1104 52-53 0.600548697852922 1104 54-55 0.6827406199492145 1104 56-57 0.6193744236858976 1104 58-59 0.5255755023695983 1104 60-61 0.6650582335271125 1104 62-63 0.6301512645567016 1104 64-65 0.4561543642822983 1104 66-67 0.529893974928676 1104 68-69 0.5238281338065036 1104 70-71 0.6404813396720357 1104 72-73 0.7067713798985267 1104 74-75 0.8105507572032806 1104 76-77 0.8560845800191714 1104 78-79 1.010762431352557 1104 80-81 0.8806133753626497 1104 82-83 0.9371476805970964 1104 84-85 0.9501221304207732 1104 86-87 0.8491069355923386 1104 88-89 0.9869956274459426 1104 90-91 0.9463596941181187 1104 92-93 0.9218688654882214 1104 94-95 0.9393753103813367 1104 96-97 0.9853528507820855 1104 98-99 1.1509939590220348 1104 100-101 1.5762164486560089 1105 1 0.16737714336976595 1105 2 0.1979304501210386 1105 3 0.20915889694350653 1105 4 0.017104044924998618 1105 5 0.13349430361068926 1105 6 0.15667655373276546 1105 7 0.1667446014788112 1105 8 0.26324351789559586 1105 9 0.2876729783323384 1105 10-11 0.2501741388810288 1105 12-13 0.22922229946136952 1105 14-15 0.38617179562892545 1105 16-17 0.2765777165569503 1105 18-19 0.28832902566185226 1105 20-21 0.2888170391775162 1105 22-23 0.4200590597531928 1105 24-25 0.363490282944511 1105 26-27 0.25415621202004957 1105 28-29 0.28472407434805547 1105 30-31 0.3922691520368815 1105 32-33 0.3508319543153604 1105 34-35 0.37871347207370576 1105 36-37 0.34460565422136114 1105 38-39 0.2039587097693456 1105 40-41 0.31011729037415847 1105 42-43 0.19989188273630276 1105 44-45 0.3316073234419079 1105 46-47 0.2856199111203779 1105 48-49 0.39136546486849255 1105 50-51 0.2877677642391987 1105 52-53 0.22807501416036757 1105 54-55 0.2398961395810275 1105 56-57 0.40201639011449686 1105 58-59 0.37585185515467145 1105 60-61 0.19507242022152838 1105 62-63 0.004398071106329837 1105 64-65 0.02424996262020329 1105 66-67 0.21858936599664247 1105 68-69 0.17554735321525072 1105 70-71 0.12368895534461899 1105 72-73 0.12116587911527432 1105 74-75 0.2576571293846115 1105 76-77 0.22440617918119088 1105 78-79 0.1712408273981687 1105 80-81 0.266571104533071 1105 82-83 0.4360117929884062 1105 84-85 0.41450878855245676 1105 86-87 0.40051781743521175 1105 88-89 0.39834905863202863 1105 90-91 0.32060307754738915 1105 92-93 0.3565182352703715 1105 94-95 0.3677163507275161 1105 96-97 0.2508890569121256 1105 98-99 0.5234331975660282 1105 100-101 0.9251547892727423 1106 1 0.19101738828680936 1106 2 0.25031685372865553 1106 3 0.2336142682472655 1106 4 0.07790310047364812 1106 5 0.16748191453211803 1106 6 0.19202839556032814 1106 7 0.21027666838236314 1106 8 0.2173226251742335 1106 9 0.19781540429169553 1106 10-11 0.3163304388391168 1106 12-13 0.25160288976452705 1106 14-15 0.3610189857597845 1106 16-17 0.3903273400612406 1106 18-19 0.39687969092715036 1106 20-21 0.2564423569609673 1106 22-23 0.3534251104763868 1106 24-25 0.388075295150351 1106 26-27 0.36634645872742055 1106 28-29 0.3802139189685434 1106 30-31 0.3516287828499216 1106 32-33 0.3345638114344496 1106 34-35 0.286538000848509 1106 36-37 0.5081135306170239 1106 38-39 0.4737547491397436 1106 40-41 0.5553774989369842 1106 42-43 0.5883726409091565 1106 44-45 0.4537964011695763 1106 46-47 0.5058502106529943 1106 48-49 0.6162890599339264 1106 50-51 0.481207471214951 1106 52-53 0.3472143712847213 1106 54-55 0.4643538277814869 1106 56-57 0.44844568369269666 1106 58-59 0.586797085822802 1106 60-61 0.3134697911756419 1106 62-63 0.27330968781578946 1106 64-65 0.32135984460590805 1106 66-67 0.4241556576770833 1106 68-69 0.48360625715460515 1106 70-71 0.22020418472792613 1106 72-73 0.25268862788153257 1106 74-75 0.4261315371542196 1106 76-77 0.5159816794729117 1106 78-79 0.5512915569046903 1106 80-81 0.5567912202515295 1106 82-83 0.7124184652296641 1106 84-85 0.6801157733641467 1106 86-87 0.5549300312721392 1106 88-89 0.6004051464887503 1106 90-91 0.690825847165712 1106 92-93 0.8107711624587317 1106 94-95 0.8509795101742448 1106 96-97 0.8276857049944688 1106 98-99 0.8405991905774215 1106 100-101 1.1671550715128873 1107 1 0.17454793867615237 1107 2 0.22042067176380087 1107 3 0.24827232588744508 1107 4 0.1347702769980046 1107 5 0.14685805849986622 1107 6 0.2388147545150332 1107 7 0.15240301086603836 1107 8 0.22706359612245564 1107 9 0.21792069671565173 1107 10-11 0.2296395886854583 1107 12-13 0.2417711912475511 1107 14-15 0.3017519781582578 1107 16-17 0.20340300990767446 1107 18-19 0.28050633987307094 1107 20-21 0.3295601943274491 1107 22-23 0.3123341575367604 1107 24-25 0.40830775360944216 1107 26-27 0.35226572962108804 1107 28-29 0.2803238135918633 1107 30-31 0.31013095125461376 1107 32-33 0.349365200729963 1107 34-35 0.22486731822754535 1107 36-37 0.360251025798938 1107 38-39 0.33384788838733215 1107 40-41 0.2121707453937134 1107 42-43 0.27714090490058396 1107 44-45 0.3236216650325261 1107 46-47 0.27698236222859407 1107 48-49 0.3269912797315939 1107 50-51 0.3728394721922612 1107 52-53 0.1284987429739246 1107 54-55 0.3272494642224828 1107 56-57 0.2807647603882941 1107 58-59 0.3129444236031773 1107 60-61 0.2108807644197057 1107 62-63 0.1616666499850794 1107 64-65 0.05505178791354837 1107 66-67 0.0909818040670487 1107 68-69 0.20308972609660714 1107 70-71 0.05654423565752609 1107 72-73 0.18505114639037146 1107 74-75 0.3691304018748305 1107 76-77 0.46658349339240246 1107 78-79 0.5718275288323298 1107 80-81 0.46539770166475236 1107 82-83 0.43910827277980147 1107 84-85 0.48898727616654014 1107 86-87 0.5230732281262114 1107 88-89 0.583648928253929 1107 90-91 0.5751663109241818 1107 92-93 0.6510097606941017 1107 94-95 0.6138050078331254 1107 96-97 0.6793440764688405 1107 98-99 0.5359820893522098 1107 100-101 1.0428210213457554 1108 1 0.2315115859128909 1108 2 0.35266153057553495 1108 3 0.33483625019399454 1108 4 0.09432951266568779 1108 5 0.2385444156468708 1108 6 0.18785628576193858 1108 7 0.28199672323075475 1108 8 0.3070447133262135 1108 9 0.29230262209785707 1108 10-11 0.2600183067403208 1108 12-13 0.3440115113608897 1108 14-15 0.46901243076086985 1108 16-17 0.4994585054382199 1108 18-19 0.4199761712364847 1108 20-21 0.4601275440578618 1108 22-23 0.5117636279591764 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-1.096708440113396 2316 66-67 -0.7519682656860667 2316 68-69 -0.7545616562906332 2316 70-71 -0.6914086055236908 2316 72-73 -0.6698716028003346 2316 74-75 -0.5722555431354159 2316 76-77 -1.0514430930714802 2316 78-79 -0.8238172736126863 2316 80-81 -0.7794352208116955 2316 82-83 -0.9488097303402547 2316 84-85 -1.0979702676442962 2316 86-87 -0.9809331076663987 2316 88-89 -1.1476141006200606 2316 90-91 -0.7210360489688519 2316 92-93 -1.0061780536004221 2316 94-95 -0.6674233232007687 2316 96-97 -0.429623638043271 2316 98-99 -0.31862028219842387 2316 100-101 -0.6709156116000372 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5.0 3 20.0 4 43.0 5 75.0 6 193.0 7 446.0 8 1153.0 9 2078.0 10 3318.0 11 5052.0 12 6833.0 13 8712.0 14 10493.0 15 12946.0 16 14956.0 17 16924.0 18 15838.0 19 14381.0 20 14782.0 21 16315.0 22 18256.0 23 21323.0 24 26054.0 25 33328.0 26 42490.0 27 54222.0 28 68801.0 29 85852.0 30 104895.0 31 126299.0 32 151314.0 33 182902.0 34 223981.0 35 277470.0 36 356903.0 37 447749.0 38 390690.0 39 75891.0 40 6.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.536818112652256 22.140400663594885 15.023384340982421 23.299396882770434 2 15.244732189092995 22.142261340182348 40.08824132615438 22.52476514457027 3 17.963795378442853 26.869446058417918 31.36939628606466 23.797362277074573 4 12.822633269397542 18.264955466227498 39.28594128265174 29.626469981723215 5 12.620201896120712 38.54410009100081 36.159476614770085 12.676221398108398 6 29.776571670415947 39.34191061101897 17.947651755795736 12.933865962769358 7 25.600558279612102 33.257524120284266 23.827589870627808 17.314327729475828 8 24.308036494317488 37.18058912336052 21.101176178234365 17.410198204087628 9 25.1963562159966 16.242068006617746 21.46323194336441 37.09834383402124 10-11 23.804540010568342 27.054393786915515 29.68438987938181 19.456676323134328 12-13 24.262307407476698 24.539594047135374 29.71441117491102 21.483687370476908 14-15 21.59277709867564 26.42205811600398 26.809246347232552 25.17591843808783 16-17 21.43176341313009 29.409168196558475 27.61147325316124 21.547595137150196 18-19 21.364166962879136 28.542521697048596 29.29642508318952 20.79688625688275 20-21 21.986354341651168 27.640788580541614 29.235094100259477 21.13776297754774 22-23 21.815160915760863 27.518396209515146 29.014801860296995 21.651641014426993 24-25 21.73686364673663 27.714392164967595 28.959940782963173 21.588803405332605 26-27 21.75203310936493 27.80699876399376 28.854047660104108 21.5869204665372 28-29 21.708321787796265 27.98560528912084 28.77742028284654 21.52865264023636 30-31 22.047585292961013 27.727987415833145 28.72251008378384 21.501917207422004 32-33 21.902659788376415 27.82646428176746 28.639145031958208 21.63173089789792 34-35 22.04996287840462 27.796449389126458 28.733156274679327 21.420431457789586 36-37 22.045877448697222 27.654533128859367 28.755572130285618 21.544017292157797 38-39 21.969063866114855 27.658014190430485 28.777459520774258 21.595462422680402 40-41 21.940559563983452 27.645274134108977 28.807714301851096 21.60645200005648 42-43 21.931852380065642 27.68999001852442 28.57736173096967 21.800795870440272 44-45 21.873643104907803 27.797383747406236 28.51555412153685 21.813419026149113 46-47 22.06924025848294 27.798688875635225 28.309198151131433 21.822872714750403 48-49 22.04046892797073 27.782342497034207 28.261387964520985 21.915800610474083 50-51 22.014831345829247 27.85888028378853 28.10597506770659 22.020313302675635 52-53 22.085546048790928 27.84555178416352 28.132496995462912 21.936405171582642 54-55 21.953376989080887 27.772056414485764 28.249291076603267 22.025275519830085 56-57 22.014724164182425 27.883438024733024 28.11046600396337 21.99137180712118 58-59 22.0022807553325 27.86193309902044 28.171600359413144 21.964185786233912 60-61 22.06946543856923 27.970301750321386 28.10966349753486 21.85056931357453 62-63 21.97397081919312 27.892809381387384 28.11435950796475 22.018860291454747 64-65 21.78928905585283 27.81954820801144 28.094886776399292 22.296275959736437 66-67 21.835943939040853 27.806753753012558 28.099701539897243 22.257600768049347 68-69 21.86076069460117 27.824911234982558 28.09338378412376 22.220944286292514 70-71 21.840592803219675 27.613449790114224 28.159848838429465 22.386108568236637 72-73 21.90551984080517 27.72878737222035 28.024601937868876 22.34109084910561 74-75 21.870329382950832 27.934923524631312 27.874976843205634 22.319770249212226 76-77 21.846440683186877 27.781936911730625 27.939366376939567 22.43225602814293 78-79 21.73142484465362 27.821726518008354 27.953187369662164 22.49366126767586 80-81 21.817448565190762 27.893118669375266 27.888141534193096 22.401291231240876 82-83 21.87687633097058 27.74071837200921 27.98012982048289 22.402275476537326 84-85 21.941525364200142 27.801466931216464 27.71724493106045 22.53976277352295 86-87 21.80661485095777 27.898025724773373 27.789959650390454 22.505399773878402 88-89 21.822608246287306 27.72966596352115 27.74069673531513 22.70702905487641 90-91 21.813394370985183 27.65437359038996 27.82789528474961 22.70433675387525 92-93 21.846309353103447 27.596755922975504 27.80082145201803 22.75611327190302 94-95 21.679441992138894 27.597332801493856 27.81163351788869 22.911591688478556 96-97 21.63776491896015 27.492905902564395 27.838371416196818 23.03095776227864 98-99 21.56642683752037 27.490805647321608 27.72598128690228 23.21678622825574 100-101 21.58854128978263 27.470350220209117 27.66696234965967 23.274146140348588 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 176.0 1 133.5 2 120.0 3 167.5 4 156.0 5 142.5 6 214.0 7 454.5 8 745.0 9 895.0 10 814.5 11 689.5 12 754.0 13 876.5 14 999.5 15 1161.0 16 1376.5 17 1516.5 18 1712.5 19 2077.0 20 2414.0 21 2839.5 22 3487.0 23 4505.0 24 6121.5 25 8624.0 26 12771.0 27 18814.5 28 26800.0 29 38117.5 30 50911.5 31 62551.0 32 73636.0 33 80892.0 34 85088.5 35 88283.5 36 92021.0 37 96102.5 38 95739.0 39 92532.0 40 89869.0 41 88860.5 42 89122.5 43 90639.0 44 91675.5 45 93473.5 46 95800.5 47 96873.0 48 97977.5 49 97296.5 50 95224.5 51 92863.5 52 90750.0 53 88966.5 54 85645.5 55 79698.0 56 71578.0 57 62820.0 58 54902.0 59 47227.0 60 39348.0 61 32279.5 62 26222.5 63 20165.0 64 14912.0 65 11106.0 66 8030.5 67 6115.0 68 4670.5 69 3369.0 70 2490.0 71 1774.0 72 1220.0 73 884.5 74 622.5 75 430.0 76 296.5 77 182.5 78 119.0 79 68.0 80 28.5 81 15.5 82 13.5 83 9.5 84 4.0 85 1.5 86 2.0 87 1.5 88 0.0 89 0.0 90 0.0 91 0.5 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.01899054320366228 2 0.00268267896557311 3 0.0032121550771993823 4 0.03462773770035817 5 0.0016590251497623182 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 1.7649203720875723E-5 24-25 7.236173525559048E-4 26-27 0.002629731354410483 28-29 1.4119362976700578E-4 30-31 0.007871544859510573 32-33 0.024567691579459008 34-35 0.014331153421351089 36-37 0.0010060046120899163 38-39 8.295125748811591E-4 40-41 0.0024355901134808498 42-43 0.008524565397182975 44-45 0.008295125748811591 46-47 0.008100984507881957 48-49 0.00961881602787727 50-51 0.19525314076404815 52-53 0.050759109901238594 54-55 0.018531663906919512 56-57 0.010536574621362808 58-59 0.020967254020400363 60-61 0.05326529682960294 62-63 0.09544689372249592 64-65 0.10014158191224887 66-67 0.10457153204618866 68-69 0.10252422441456709 70-71 0.09318779564622383 72-73 0.07033207682768976 74-75 0.2521894719675932 76-77 0.301783734423254 78-79 0.007289121136721674 80-81 0.0012001458530195492 82-83 0.0 84-85 0.0 86-87 0.0 88-89 1.7649203720875725E-4 90-91 0.007130278303233793 92-93 0.019961249408310445 94-95 0.0019061140018545781 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2832989.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.724678686517905 #Duplication Level Percentage of deduplicated Percentage of total 1 78.42776363810349 47.625007470260066 2 12.341838323678186 14.98908333212621 3 3.81331870870191 6.946876599456325 4 1.7338057564033595 4.211391898497165 5 0.9576457845280332 2.9076366280481585 6 0.6122106315518488 2.230577633367373 7 0.4148990954187003 1.7636229979639255 8 0.3002354320086981 1.4585360111228869 9 0.2269883761716114 1.2405416587736005 >10 1.0996342078355814 11.613278290088154 >50 0.049739115518095546 2.0452906218813633 >100 0.02069939839592974 2.212468627483787 >500 9.306908073837943E-4 0.3777381481798705 >1k 2.9084087725376887E-4 0.37795008275099834 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3547 0.1252034511958924 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3041 0.10734245703036616 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 3.529840744175145E-5 0.0 0.0 0.0 6 0.0 3.529840744175145E-5 0.0 0.0 3.529840744175145E-5 7 3.529840744175145E-5 3.529840744175145E-5 0.0 0.0 3.529840744175145E-5 8 3.529840744175145E-5 3.529840744175145E-5 0.0 0.0 3.529840744175145E-5 9 3.529840744175145E-5 3.529840744175145E-5 0.0 0.0 3.529840744175145E-5 10-11 5.294761116262718E-5 3.529840744175145E-5 0.0 0.0 7.05968148835029E-5 12-13 7.05968148835029E-5 3.529840744175145E-5 0.0 0.0 2.647380558131359E-4 14-15 7.05968148835029E-5 3.529840744175145E-5 0.0 0.0 3.3533487069663877E-4 16-17 1.0589522232525435E-4 3.529840744175145E-5 0.0 0.0 4.5887929674276885E-4 18-19 1.0589522232525435E-4 3.529840744175145E-5 0.0 0.0 4.5887929674276885E-4 20-21 1.0589522232525435E-4 3.529840744175145E-5 0.0 0.0 4.765285004636446E-4 22-23 1.411936297670058E-4 3.529840744175145E-5 0.0 8.824601860437863E-5 5.647745190680232E-4 24-25 1.411936297670058E-4 3.529840744175145E-5 0.0 3.3533487069663877E-4 6.000729265097746E-4 26-27 1.7649203720875725E-4 3.529840744175145E-5 0.0 6.883189451141533E-4 6.883189451141532E-4 28-29 2.117904446505087E-4 3.529840744175145E-5 0.0 0.0018178679832501997 7.05968148835029E-4 30-31 2.117904446505087E-4 7.05968148835029E-5 0.0 0.006371362543236137 7.05968148835029E-4 32-33 2.117904446505087E-4 1.0589522232525435E-4 0.0 0.020296584279007084 7.05968148835029E-4 34-35 2.117904446505087E-4 1.0589522232525435E-4 0.0 0.04138738272545357 7.412665562767805E-4 36-37 2.4708885209226016E-4 1.5884283348788155E-4 0.0 0.07571508396255686 7.412665562767805E-4 38-39 2.4708885209226016E-4 1.7649203720875725E-4 0.0 0.12345618002752569 7.765649637185319E-4 40-41 2.4708885209226016E-4 1.7649203720875725E-4 0.0 0.1749212580775993 8.471617786020348E-4 42-43 2.4708885209226016E-4 1.7649203720875725E-4 0.0 0.2261039488681389 8.648109823229104E-4 44-45 2.4708885209226016E-4 1.7649203720875725E-4 0.0 0.2915824946725879 9.00109389764662E-4 46-47 2.4708885209226016E-4 1.7649203720875725E-4 0.0 0.3743925585309368 9.354077972064134E-4 48-49 2.4708885209226016E-4 1.7649203720875725E-4 0.0 0.45681433990742637 0.0010766014269734192 50-51 2.4708885209226016E-4 1.9414124092963296E-4 0.0 0.5422364859164649 0.0011648474455777979 52-53 2.4708885209226016E-4 2.117904446505087E-4 0.0 0.630994331428749 0.0012001458530195492 54-55 2.647380558131359E-4 3.000364632548873E-4 0.0 0.7285061819865873 0.001217795056740425 56-57 2.823872595340116E-4 3.8828248185926597E-4 0.0 0.8436672362653015 0.0012707426679030524 58-59 2.823872595340116E-4 3.8828248185926597E-4 0.0 0.9749243643374542 0.0013236902790656794 60-61 2.823872595340116E-4 4.4123009302189317E-4 0.0 1.1136294563798166 0.001341339482786555 62-63 2.823872595340116E-4 5.294761116262718E-4 0.0 1.256023231999842 0.0013766378902283067 64-65 2.823872595340116E-4 5.647745190680232E-4 0.0 1.4064473953128656 0.0013942870939491823 66-67 2.823872595340116E-4 6.000729265097746E-4 0.0 1.5784388855727998 0.0014472347051118096 68-69 2.823872595340116E-4 6.000729265097746E-4 0.0 1.7740273612075443 0.001482533112553561 70-71 2.823872595340116E-4 6.000729265097746E-4 0.0 1.9870885485259562 0.0015178315199953123 72-73 2.823872595340116E-4 6.000729265097746E-4 0.0 2.2058857270536523 0.0015354807237161882 74-75 2.823872595340116E-4 6.000729265097746E-4 0.0 2.436225484814802 0.0015884283348788152 76-77 2.823872595340116E-4 6.000729265097746E-4 0.0 2.6986691441442234 0.0015884283348788152 78-79 2.823872595340116E-4 6.000729265097746E-4 0.0 2.990057497575882 0.0015884283348788152 80-81 2.823872595340116E-4 6.000729265097746E-4 0.0 3.3010894147488745 0.0016413759460414423 82-83 2.823872595340116E-4 6.000729265097746E-4 0.0 3.63111187512553 0.001800218779529324 84-85 2.823872595340116E-4 6.000729265097746E-4 0.0 3.9750419080342354 0.001800218779529324 86-87 2.823872595340116E-4 6.000729265097746E-4 0.0 4.354640981662831 0.001923763205575454 88-89 2.823872595340116E-4 6.000729265097746E-4 0.0 4.763272995412266 0.002047307631621584 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2870 0.0 51.625607 1 GTATCAA 5730 0.0 42.267647 1 CAACGCA 6900 0.0 34.109264 5 ATCAACG 6970 0.0 33.78698 3 TCAACGC 6945 0.0 33.77128 4 AACGCAG 7245 0.0 32.680916 6 TATCAAC 7385 0.0 32.14548 2 ACGCAGA 8320 0.0 28.116137 7 CGCAGAG 8725 0.0 26.593502 8 GTACATG 7240 0.0 23.350918 1 GCAGAGT 9780 0.0 23.094053 9 TACATGG 7390 0.0 22.422367 2 ACATGGG 7430 0.0 21.662636 3 AGAGTAC 8965 0.0 20.721136 10-11 CGTATAC 290 2.4556357E-9 19.646431 3 CAGAGTA 9375 0.0 19.308804 10-11 CATGGGG 5395 0.0 19.272827 4 TGGTATC 1600 0.0 18.99155 2 GAGTACT 6000 0.0 18.861195 12-13 GTGGTAT 1790 0.0 18.836397 1 >>END_MODULE