FastQCFastQC Report
Sat 18 Jun 2016
SRR1930913_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1930913_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2123190
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN38950.18345037420108423No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA18300.056.81531
GTATCAA33350.042.0198441
ATCAACG37650.033.8739363
TCAACGC37450.033.2379464
TATCAAC40450.033.145942
CAACGCA37700.031.87095
AACGCAG40850.029.7020726
GTACATG40350.028.8795531
TACATGG42050.026.9885792
ACGCAGA47700.025.3567527
ACATGGG43350.024.9026893
CGCAGAG49850.023.959018
GTATTAG5150.023.4072321
ATAACGC1956.39477E-620.5992933
CATGGGG32000.020.233644
GCAGAGT55050.020.1957749
GAGTACT35250.018.4696612-13
CAGAGTA54700.018.42146310-11
GTGTAAG8500.018.3184551
AGAGTAC50850.017.8563610-11
Produced by FastQC (version 0.11.5)