##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1930913_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2123190 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 23.132153033878268 30.0 16.0 31.0 2.0 31.0 2 22.615380159100223 30.0 10.0 31.0 2.0 33.0 3 23.26684517165209 30.0 16.0 31.0 2.0 33.0 4 25.013951177238024 33.0 10.0 35.0 2.0 37.0 5 24.926732416787946 33.0 10.0 35.0 2.0 37.0 6 25.35815494609526 32.0 17.0 35.0 2.0 37.0 7 24.874769097443 32.0 17.0 35.0 2.0 37.0 8 24.772837569883052 32.0 17.0 35.0 2.0 37.0 9 23.3535411338599 28.0 10.0 34.0 2.0 37.0 10-11 23.273082248880222 27.5 10.5 34.5 2.0 37.0 12-13 22.969845138682828 27.0 10.0 34.0 2.0 37.0 14-15 25.042880288622307 31.0 16.5 35.0 2.0 38.0 16-17 26.91535920007159 32.5 17.0 37.0 2.0 38.5 18-19 27.784989803079327 34.0 18.5 38.0 2.0 39.5 20-21 27.95251743838281 35.0 18.0 39.0 2.0 40.0 22-23 28.333223592801396 35.5 19.5 39.0 2.0 40.0 24-25 28.295978692439206 36.0 18.5 39.0 2.0 40.0 26-27 28.154799853051305 35.5 18.5 39.0 2.0 40.0 28-29 28.29792976605956 35.5 20.0 39.0 2.0 40.0 30-31 28.395560689340094 35.5 21.0 39.0 2.0 40.0 32-33 28.29371370437879 35.0 22.5 39.0 2.0 40.0 34-35 24.910306896697893 33.0 8.5 38.0 2.0 40.0 36-37 23.170789001455354 27.5 8.5 37.5 2.0 40.0 38-39 22.13867976959198 27.5 2.0 37.0 2.0 40.0 40-41 24.034770086520755 30.5 6.0 37.5 2.0 40.0 42-43 24.05432274078156 31.0 5.5 38.0 2.0 40.0 44-45 21.70927495890617 24.0 2.0 37.5 2.0 39.5 46-47 23.08329212176018 27.0 8.0 37.0 2.0 40.0 48-49 22.52243981932846 30.0 2.0 37.0 2.0 39.5 50-51 19.83979059810945 16.5 5.0 35.5 2.0 38.5 52-53 23.636033044616827 28.5 8.0 36.5 2.0 39.0 54-55 25.839720656182443 33.0 7.0 38.0 2.0 40.0 56-57 26.19740932276433 34.0 5.0 38.0 2.0 40.0 58-59 25.81700954695529 33.5 4.5 38.0 2.0 40.0 60-61 25.604179089012288 33.0 2.0 38.0 2.0 40.0 62-63 25.192969305620316 32.5 2.0 37.5 2.0 40.0 64-65 24.73127369665456 32.0 2.0 37.0 2.0 39.5 66-67 24.35088216316015 31.5 2.0 36.5 2.0 39.0 68-69 23.9451370343681 31.0 2.0 36.0 2.0 39.0 70-71 23.359882299747078 31.0 2.0 35.5 2.0 38.5 72-73 22.968063150259752 30.0 2.0 35.0 2.0 38.0 74-75 22.445729539042667 30.0 2.0 35.0 2.0 37.0 76-77 21.92344514621866 29.0 2.0 34.0 2.0 36.5 78-79 21.49533838233978 29.0 2.0 34.0 2.0 36.0 80-81 21.050717081372838 29.0 2.0 34.0 2.0 36.0 82-83 20.623788262001987 27.5 2.0 34.0 2.0 35.0 84-85 20.140877406167135 26.5 2.0 34.0 2.0 35.0 86-87 19.605186770849524 26.0 2.0 34.0 2.0 35.0 88-89 19.080305577927554 25.5 2.0 33.0 2.0 35.0 90-91 18.53772978395716 25.0 2.0 33.0 2.0 35.0 92-93 17.91710845473085 23.5 2.0 32.5 2.0 35.0 94-95 17.256325152247328 19.5 2.0 32.0 2.0 34.0 96-97 16.480019451862525 12.5 2.0 32.0 2.0 34.0 98-99 15.570910045733072 2.0 2.0 32.0 2.0 34.0 100-101 14.076278853988573 2.0 2.0 30.5 2.0 34.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 7.781188476616489 1101 2 8.56295525305157 1101 3 8.071871654023763 1101 4 10.331417357975266 1101 5 10.100735419017106 1101 6 9.657071954406959 1101 7 9.981058396378458 1101 8 9.653821124889475 1101 9 11.282245466821347 1101 10-11 10.393065425378595 1101 12-13 9.581122615830864 1101 14-15 8.389831075855792 1101 16-17 7.667118265306307 1101 18-19 7.735526941371319 1101 20-21 8.303673606185349 1101 22-23 8.064279559316265 1101 24-25 8.428159005414894 1101 26-27 8.531270947501799 1101 28-29 8.367327213280344 1101 30-31 8.25937351296416 1101 32-33 8.033565478484434 1101 34-35 11.040410448764657 1101 36-37 4.4259931403762 1101 38-39 8.050084497335625 1101 40-41 9.84417806195136 1101 42-43 10.418178743459766 1101 44-45 3.681959415321426 1101 46-47 5.422657587702158 1101 48-49 9.61082396279497 1101 50-51 -6.469122372203724 1101 52-53 4.920850430211665 1101 54-55 7.682049314596277 1101 56-57 8.224969450273775 1101 58-59 8.525668754695623 1101 60-61 8.272048384517984 1101 62-63 8.197967024773448 1101 64-65 8.41864146921517 1101 66-67 8.392701604836272 1101 68-69 8.388524569184774 1101 70-71 8.522216779166829 1101 72-73 8.3699596591482 1101 74-75 8.461258059284148 1101 76-77 8.441209859632473 1101 78-79 8.55756009033498 1101 80-81 8.27247858677919 1101 82-83 8.15481434075459 1101 84-85 8.163763533157613 1101 86-87 8.00489878397719 1101 88-89 8.206450849205197 1101 90-91 7.929805615622509 1101 92-93 8.156803469559915 1101 94-95 8.027948883607259 1101 96-97 8.318669020732539 1101 98-99 8.218774218657154 1101 100-101 8.013258757135999 1102 1 7.354360404345055 1102 2 8.010447908665622 1102 3 7.535502303446201 1102 4 9.798229730342623 1102 5 9.751765132524255 1102 6 9.03401818122235 1102 7 9.322967981303627 1102 8 8.864488589654862 1102 9 9.556719830061365 1102 10-11 9.12161797446511 1102 12-13 8.241762137684894 1102 14-15 7.283654496919201 1102 16-17 6.893459190709066 1102 18-19 6.935576606400858 1102 20-21 7.614109726339464 1102 22-23 7.441502074453869 1102 24-25 7.803204850836895 1102 26-27 7.945253689765227 1102 28-29 7.611883411358502 1102 30-31 7.52576195955308 1102 32-33 7.249019306622763 1102 34-35 10.357672622978473 1102 36-37 -0.25205973920261826 1102 38-39 2.0870846403936056 1102 40-41 8.013634696943992 1102 42-43 9.36797998202577 1102 44-45 -0.22554225335061417 1102 46-47 -0.09420947496088772 1102 48-49 7.392006651077306 1102 50-51 -7.525807475653284 1102 52-53 3.595443272206353 1102 54-55 6.659806534262415 1102 56-57 7.314393149509982 1102 58-59 7.603438193053236 1102 60-61 7.561090852474816 1102 62-63 7.472362159018978 1102 64-65 7.39228972327966 1102 66-67 7.295423394349758 1102 68-69 7.26194905706355 1102 70-71 7.385274527985388 1102 72-73 7.421601968809156 1102 74-75 7.379654525225202 1102 76-77 7.297287959563132 1102 78-79 7.075567158939183 1102 80-81 7.099311664053598 1102 82-83 7.045791090388132 1102 84-85 7.133487013835264 1102 86-87 7.003511466046838 1102 88-89 7.03463993781239 1102 90-91 7.234732382500006 1102 92-93 7.245873576480555 1102 94-95 7.181500145982483 1102 96-97 7.187101980642666 1102 98-99 6.924059886800736 1102 100-101 6.63242575037742 1103 1 7.611378406951047 1103 2 8.204975329178488 1103 3 7.713516658149352 1103 4 9.962725409991162 1103 5 9.631014874866437 1103 6 8.856476031304581 1103 7 8.817853503875583 1103 8 8.47402720690065 1103 9 8.802149878885405 1103 10-11 7.879622691678108 1103 12-13 6.984539459284555 1103 14-15 6.698686644717686 1103 16-17 6.866573937277728 1103 18-19 7.32667941408539 1103 20-21 7.930691406888897 1103 22-23 8.080595392917314 1103 24-25 8.264360568020077 1103 26-27 8.354594802927569 1103 28-29 8.360501043936441 1103 30-31 8.08094767060404 1103 32-33 7.917002935782456 1103 34-35 10.79441007470037 1103 36-37 -4.291211640068621 1103 38-39 -2.4766450763560464 1103 40-41 7.48313256436726 1103 42-43 9.778724212223185 1103 44-45 0.11454974600151502 1103 46-47 -0.7399305824342086 1103 48-49 8.06997487314571 1103 50-51 -7.697594126117796 1103 52-53 3.8556260944276985 1103 54-55 7.319021135221163 1103 56-57 7.76529755060362 1103 58-59 8.052950758454806 1103 60-61 8.000240295619673 1103 62-63 7.939541710456666 1103 64-65 8.015241051536922 1103 66-67 7.9450837050540635 1103 68-69 7.876098318901104 1103 70-71 8.00130636393532 1103 72-73 7.920648896778587 1103 74-75 7.804499023999163 1103 76-77 7.826918004035797 1103 78-79 7.91752078133813 1103 80-81 7.654484079236031 1103 82-83 7.423057129436085 1103 84-85 7.688129004444349 1103 86-87 7.468160169055011 1103 88-89 7.5883085096830065 1103 90-91 7.647403909276676 1103 92-93 7.817000026636762 1103 94-95 7.500187811526118 1103 96-97 7.36793244059108 1103 98-99 7.47745005124243 1103 100-101 7.08051827672943 1104 1 7.112745427415533 1104 2 7.886375176973338 1104 3 7.258736266474244 1104 4 9.378513142287499 1104 5 9.035098156751708 1104 6 8.241232830789016 1104 7 8.170618178151457 1104 8 7.64381954773971 1104 9 7.40101885882439 1104 10-11 6.5592757911016655 1104 12-13 6.018632941819227 1104 14-15 6.223473555545798 1104 16-17 6.603449775475568 1104 18-19 7.291695876811019 1104 20-21 7.929865425100928 1104 22-23 7.978924766745013 1104 24-25 8.051180536173955 1104 26-27 8.406185107326092 1104 28-29 8.151514993981909 1104 30-31 7.794843788823243 1104 32-33 7.770217985017567 1104 34-35 10.970657299350538 1104 36-37 -4.469013394234931 1104 38-39 -2.579744931647337 1104 40-41 7.202555342625395 1104 42-43 9.630805278145917 1104 44-45 -0.04518765497977384 1104 46-47 -1.0712997968821618 1104 48-49 8.199721815403908 1104 50-51 -7.613689663696729 1104 52-53 4.0575087795023705 1104 54-55 7.144825520597699 1104 56-57 7.545647916810466 1104 58-59 7.91704162704854 1104 60-61 7.748217533139378 1104 62-63 7.9291635603340325 1104 64-65 8.02310671773667 1104 66-67 7.843762094944939 1104 68-69 7.799726990928836 1104 70-71 7.946311067851564 1104 72-73 7.883091059410454 1104 74-75 7.918784073866171 1104 76-77 7.803915814369656 1104 78-79 7.610257322387795 1104 80-81 7.572954633348335 1104 82-83 7.501820154495771 1104 84-85 7.4971850342291475 1104 86-87 7.297771547469274 1104 88-89 7.242912617338124 1104 90-91 7.312680039168345 1104 92-93 7.319594637758097 1104 94-95 7.192549628835195 1104 96-97 7.250489369359617 1104 98-99 7.0014069573254165 1104 100-101 6.814154653210709 1105 1 6.509034898343128 1105 2 7.395175850486353 1105 3 7.039263166258145 1105 4 9.120809643201166 1105 5 8.655572991186226 1105 6 7.440437495720168 1105 7 7.427164949673035 1105 8 6.557370311455362 1105 9 6.047908904721041 1105 10-11 5.343909141634928 1105 12-13 4.785414511771858 1105 14-15 5.265251439799325 1105 16-17 6.01877840404633 1105 18-19 6.956944583906569 1105 20-21 7.670166216944281 1105 22-23 7.560230318959729 1105 24-25 7.853257621600701 1105 26-27 7.973048150627239 1105 28-29 7.725624121712723 1105 30-31 7.318970860089152 1105 32-33 7.157591070878837 1105 34-35 10.120634118243043 1105 36-37 -4.780514133314593 1105 38-39 -2.9050666543818906 1105 40-41 6.401157298761696 1105 42-43 8.901530837111284 1105 44-45 -4.237854568652942 1105 46-47 -5.445114941613745 1105 48-49 6.127297149153666 1105 50-51 -12.410590378722354 1105 52-53 0.6960125990505404 1105 54-55 5.563679908325483 1105 56-57 6.429960126561667 1105 58-59 6.983343086197415 1105 60-61 6.974633423546084 1105 62-63 6.90616770771544 1105 64-65 6.771699168648976 1105 66-67 6.736756841853801 1105 68-69 6.612072919007268 1105 70-71 6.76595943275905 1105 72-73 6.608331017820248 1105 74-75 6.5297983006646945 1105 76-77 6.497190383433839 1105 78-79 6.450239980203612 1105 80-81 6.473231157772883 1105 82-83 6.305594504357845 1105 84-85 6.385797775884917 1105 86-87 6.401260643619466 1105 88-89 6.4618535220026665 1105 90-91 6.329736484680133 1105 92-93 6.233634144572765 1105 94-95 5.969075120294072 1105 96-97 5.88201567555198 1105 98-99 5.80224564785472 1105 100-101 5.416698270549793 1106 1 6.351805449110628 1106 2 7.152297472212055 1106 3 6.913221668071959 1106 4 8.432721116818083 1106 5 7.901552565575656 1106 6 7.15848088858969 1106 7 6.945670460428829 1106 8 5.863977441934345 1106 9 5.083775799365867 1106 10-11 4.077995727122886 1106 12-13 4.004080993591373 1106 14-15 4.507683296666013 1106 16-17 5.832076616420309 1106 18-19 6.646448346316362 1106 20-21 7.504830064691838 1106 22-23 7.238783042759568 1106 24-25 7.29634607096413 1106 26-27 7.5869555884146145 1106 28-29 7.527944338600047 1106 30-31 6.885554923183641 1106 32-33 6.9512579377965835 1106 34-35 10.04635232645796 1106 36-37 -0.8904904882827438 1106 38-39 0.3238018499735702 1106 40-41 7.281085593469676 1106 42-43 8.72183109820789 1106 44-45 -4.385965084145443 1106 46-47 -5.5431183997959295 1106 48-49 5.752543226928104 1106 50-51 -12.333343908147711 1106 52-53 0.364622278879871 1106 54-55 5.275345111618847 1106 56-57 6.389616272344455 1106 58-59 6.844809001152321 1106 60-61 6.63059147094398 1106 62-63 6.6395950050189825 1106 64-65 6.499309171960821 1106 66-67 6.488777259762411 1106 68-69 6.392740987431523 1106 70-71 6.303549449472314 1106 72-73 6.203375381137334 1106 74-75 6.153436015645784 1106 76-77 5.982909785453295 1106 78-79 5.938718226004113 1106 80-81 5.749927900611979 1106 82-83 5.814638536490445 1106 84-85 5.657831941800644 1106 86-87 5.8237143740661 1106 88-89 5.74177892847386 1106 90-91 5.459892757545344 1106 92-93 5.5013904467054395 1106 94-95 5.3313430023307795 1106 96-97 5.227358682553064 1106 98-99 5.051735970335985 1106 100-101 4.774249854458846 1107 1 6.004097653992691 1107 2 7.11088031570246 1107 3 6.6598776583806405 1107 4 8.065661658069669 1107 5 7.723908216779954 1107 6 6.294856830992423 1107 7 6.393084043876495 1107 8 5.335135214950082 1107 9 4.204263011444528 1107 10-11 3.4921019033500933 1107 12-13 3.734354586736476 1107 14-15 4.3264129247830745 1107 16-17 6.155749413580217 1107 18-19 6.966664710817625 1107 20-21 7.792205347852409 1107 22-23 7.725687105434989 1107 24-25 7.865264791453104 1107 26-27 7.828648334150575 1107 28-29 7.581612348224066 1107 30-31 7.352665278180197 1107 32-33 7.17596984438304 1107 34-35 10.05913202638806 1107 36-37 -0.7912047565162972 1107 38-39 0.6049120382476225 1107 40-41 7.818165479547737 1107 42-43 9.162998323930257 1107 44-45 -4.2149118186023955 1107 46-47 -5.154813294569561 1107 48-49 6.368660665336378 1107 50-51 -12.267446841698852 1107 52-53 0.8958026359107407 1107 54-55 5.797914787091212 1107 56-57 6.974956393883623 1107 58-59 7.475230732588386 1107 60-61 7.375906033465679 1107 62-63 7.138797146102924 1107 64-65 7.011590998749913 1107 66-67 6.977117200813009 1107 68-69 6.8657682702678215 1107 70-71 6.990928327108708 1107 72-73 6.787888773236524 1107 74-75 6.933240529695599 1107 76-77 6.885544039125392 1107 78-79 6.732052117628374 1107 80-81 6.516006870481096 1107 82-83 6.3869182830722515 1107 84-85 6.420752361635422 1107 86-87 6.403165864702871 1107 88-89 6.274108370595915 1107 90-91 6.192591283504342 1107 92-93 6.204148709793536 1107 94-95 6.062878485925438 1107 96-97 5.9094827424446805 1107 98-99 6.031367146524952 1107 100-101 6.039356403899715 1108 1 5.500533983548582 1108 2 5.922691928098622 1108 3 6.315518947524094 1108 4 7.4948974006589 1108 5 6.967818281761094 1108 6 6.543034255855545 1108 7 5.889160536672744 1108 8 4.928178942718564 1108 9 2.4105095124901226 1108 10-11 2.9597664965045425 1108 12-13 2.6592297939447533 1108 14-15 4.119864870396654 1108 16-17 5.233744128697104 1108 18-19 6.440647499470643 1108 20-21 7.593841727933594 1108 22-23 7.516683350345428 1108 24-25 7.257179263362442 1108 26-27 7.465476164541531 1108 28-29 7.3794513394024115 1108 30-31 7.234839011890074 1108 32-33 7.04592901090286 1108 34-35 10.021140788783516 1108 36-37 -4.752314511796115 1108 38-39 -2.983157521355846 1108 40-41 6.077353402443649 1108 42-43 8.526930127262123 1108 44-45 -4.341096200049634 1108 46-47 -5.4756539636866 1108 48-49 6.046272854095125 1108 50-51 -12.455143621335289 1108 52-53 0.5972899741791267 1108 54-55 5.665201883025329 1108 56-57 6.69572462782196 1108 58-59 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-18.612340531040218 2313 80-81 -17.881339640742464 2313 82-83 -17.39814569762416 2313 84-85 -16.89816820695853 2313 86-87 -16.352686822151696 2313 88-89 -15.832359430494156 2313 90-91 -15.297651313370743 2313 92-93 -14.76685508839708 2313 94-95 -14.781146118643981 2313 96-97 -13.838435721757392 2313 98-99 -12.686169261985018 2313 100-101 -11.15123544203072 2314 1 -20.63078811050643 2314 2 -20.108363972522497 2314 3 -20.740825329317076 2314 4 -22.411491241754586 2314 5 -22.31124116810581 2314 6 -22.768412061801953 2314 7 -22.29584389988449 2314 8 -22.243072166420745 2314 9 -20.83571941386303 2314 10-11 -20.76317050438277 2314 12-13 -20.477859824511327 2314 14-15 -22.53452732126262 2314 16-17 -24.403345489758976 2314 18-19 -25.254117362996084 2314 20-21 -25.407267411374306 2314 22-23 -25.747291804934076 2314 24-25 -25.719972466894877 2314 26-27 -25.599075743178073 2314 28-29 -25.728800657048343 2314 30-31 -25.825498166459525 2314 32-33 -25.728028398147714 2314 34-35 -22.50303844812864 2314 36-37 -20.707505283757072 2314 38-39 -19.73559762783322 2314 40-41 -21.623178984424143 2314 42-43 -21.62346916288872 2314 44-45 -19.286748373962677 2314 46-47 -20.555955649578348 2314 48-49 -20.101242673855186 2314 50-51 -17.423422237129433 2314 52-53 -21.133854657586625 2314 54-55 -23.241408587249737 2314 56-57 -23.57557084688966 2314 58-59 -23.17063921468754 2314 60-61 -22.975587815391965 2314 62-63 -22.59604468166509 2314 64-65 -22.134937999492617 2314 66-67 -21.759482096199296 2314 68-69 -21.362263364178574 2314 70-71 -20.76346534600201 2314 72-73 -20.380828274215148 2314 74-75 -19.861794619689288 2314 76-77 -19.35325209444227 2314 78-79 -18.930733471123823 2314 80-81 -18.504423709483756 2314 82-83 -18.09484797351826 2314 84-85 -17.610662401106232 2314 86-87 -17.077721657265492 2314 88-89 -16.55739426560795 2314 90-91 -16.022918382576503 2314 92-93 -15.42941895277237 2314 94-95 -14.781146118643981 2314 96-97 -14.023294058961294 2314 98-99 -13.136471166304572 2314 100-101 -11.69768226042366 2315 1 -20.63078811050643 2315 2 -20.108363972522497 2315 3 -20.740825329317076 2315 4 -21.671061736619198 2315 5 -22.31124116810581 2315 6 -22.768412061801953 2315 7 -22.29584389988449 2315 8 -22.243072166420745 2315 9 -20.17698925513287 2315 10-11 -19.931237731273526 2315 12-13 -19.125618928152782 2315 14-15 -21.08471406262583 2315 16-17 -23.761655480421908 2315 18-19 -25.254117362996084 2315 20-21 -25.407267411374306 2315 22-23 -25.747291804934076 2315 24-25 -25.719972466894877 2315 26-27 -25.599075743178073 2315 28-29 -25.728800657048343 2315 30-31 -25.825498166459525 2315 32-33 -25.728028398147714 2315 34-35 -22.50303844812864 2315 36-37 -20.707505283757072 2315 38-39 -19.73559762783322 2315 40-41 -21.623178984424143 2315 42-43 -21.62346916288872 2315 44-45 -19.286748373962677 2315 46-47 -20.555955649578348 2315 48-49 -20.101242673855186 2315 50-51 -17.423422237129433 2315 52-53 -21.133854657586625 2315 54-55 -23.241408587249737 2315 56-57 -23.57557084688966 2315 58-59 -23.17063921468754 2315 60-61 -22.975587815391965 2315 62-63 -22.59604468166509 2315 64-65 -22.134937999492617 2315 66-67 -21.759482096199296 2315 68-69 -21.362263364178574 2315 70-71 -20.76346534600201 2315 72-73 -20.380828274215148 2315 74-75 -19.861794619689288 2315 76-77 -19.35325209444227 2315 78-79 -18.930733471123823 2315 80-81 -18.504423709483756 2315 82-83 -18.09484797351826 2315 84-85 -17.610662401106232 2315 86-87 -17.077721657265492 2315 88-89 -16.55739426560795 2315 90-91 -16.022918382576503 2315 92-93 -15.42941895277237 2315 94-95 -14.781146118643981 2315 96-97 -14.023294058961294 2315 98-99 -13.136471166304572 2315 100-101 -11.69768226042366 2316 1 -20.63078811050643 2316 2 -20.108363972522497 2316 3 -20.740825329317076 2316 4 -22.411491241754586 2316 5 -22.31124116810581 2316 6 -22.768412061801953 2316 7 -22.29584389988449 2316 8 -22.243072166420745 2316 9 -20.83571941386303 2316 10-11 -20.76317050438277 2316 12-13 -20.477859824511327 2316 14-15 -22.53452732126262 2316 16-17 -24.403345489758976 2316 18-19 -25.254117362996084 2316 20-21 -25.407267411374306 2316 22-23 -25.747291804934076 2316 24-25 -25.719972466894877 2316 26-27 -25.599075743178073 2316 28-29 -25.728800657048343 2316 30-31 -25.825498166459525 2316 32-33 -25.728028398147714 2316 34-35 -22.50303844812864 2316 36-37 -20.707505283757072 2316 38-39 -19.73559762783322 2316 40-41 -21.623178984424143 2316 42-43 -21.62346916288872 2316 44-45 -19.286748373962677 2316 46-47 -20.555955649578348 2316 48-49 -20.101242673855186 2316 50-51 -17.423422237129433 2316 52-53 -21.133854657586625 2316 54-55 -23.241408587249737 2316 56-57 -23.57557084688966 2316 58-59 -23.17063921468754 2316 60-61 -22.975587815391965 2316 62-63 -22.59604468166509 2316 64-65 -22.134937999492617 2316 66-67 -21.759482096199296 2316 68-69 -21.362263364178574 2316 70-71 -20.76346534600201 2316 72-73 -20.380828274215148 2316 74-75 -19.861794619689288 2316 76-77 -19.35325209444227 2316 78-79 -18.930733471123823 2316 80-81 -18.504423709483756 2316 82-83 -18.09484797351826 2316 84-85 -17.610662401106232 2316 86-87 -17.077721657265492 2316 88-89 -16.55739426560795 2316 90-91 -16.022918382576503 2316 92-93 -15.42941895277237 2316 94-95 -14.781146118643981 2316 96-97 -14.023294058961294 2316 98-99 -13.136471166304572 2316 100-101 -11.69768226042366 >>END_MODULE >>Per sequence quality scores fail #Quality Count 2 328698.0 3 7476.0 4 9073.0 5 6791.0 6 8486.0 7 11549.0 8 17387.0 9 20715.0 10 22182.0 11 21686.0 12 21583.0 13 21307.0 14 22494.0 15 26129.0 16 28605.0 17 30287.0 18 31992.0 19 34605.0 20 37271.0 21 41056.0 22 44987.0 23 49563.0 24 54840.0 25 60890.0 26 68693.0 27 77134.0 28 86490.0 29 97033.0 30 107162.0 31 111598.0 32 113751.0 33 117712.0 34 118951.0 35 112263.0 36 93089.0 37 50874.0 38 8714.0 39 74.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.635313867239226 19.920244606552462 14.462121659805163 23.982319866403152 2 14.865345210338955 20.418583150215323 41.04598907760148 23.67008256184424 3 18.035345848131236 26.188736681887363 30.37939004453464 25.396527425446756 4 12.073048388550147 16.866067942210076 40.061874868591 30.999008800648788 5 12.674501332716373 37.696483510819064 36.327205095669676 13.301810060794883 6 29.68328292812299 38.544050885118466 18.295754150350906 13.476912036407644 7 25.459807017389547 34.26969627668464 23.001455725707853 17.269040980217962 8 23.872869662978765 38.48947287732835 20.476254354362027 17.161403105330848 9 24.829543111999282 19.811899605416013 20.384543560665772 34.974013721918936 10-11 23.328469902815097 30.080334496873505 27.92395971974586 18.66723588056553 12-13 23.740061715243 27.516890269439838 28.051328281895028 20.691719733422133 14-15 21.632656164466884 27.474352736046065 26.041679338239764 24.85131176124729 16-17 21.62585643282087 29.467612650929954 27.094432177929 21.812098738320167 18-19 21.648324942446067 28.33837443390247 28.939748722209636 21.07355190144183 20-21 22.41717640568194 27.419333989913675 28.874390093979365 21.289099510425025 22-23 22.162366721762254 27.21148273029894 28.754230873978642 21.871919673960164 24-25 21.99515869638505 27.2475586234339 28.799262751253156 21.958019928927893 26-27 21.950092881154873 27.421705716902974 28.557493421422325 22.070707980519828 28-29 22.012775807065736 27.548519671387055 28.604045708090343 21.834658813456866 30-31 22.22626889391617 27.314898240982377 28.56132136917659 21.897511495924864 32-33 22.04702446300254 27.430918956778356 28.445590779282586 22.076465800936518 34-35 22.438003318448054 27.460114250665786 28.360000983672485 21.741881447213675 36-37 22.87265450929603 27.426573941651988 27.949471823421458 21.751299725630528 38-39 22.61959832979404 27.494017199867415 28.019083037987592 21.867301432350953 40-41 22.5774092880869 27.327331356399593 28.25020810329688 21.845051252216628 42-43 22.675469917711837 27.490546055285442 27.780186698481522 22.053797328521192 44-45 22.933852516963373 27.518631002438852 27.662641105076506 21.88487537552127 46-47 22.380018879072512 27.50390541359283 27.89841792513734 22.217657782197325 48-49 22.83257951443775 27.57487593418032 27.41772688896131 22.174817662420615 50-51 23.132140638264573 27.643903572631167 27.287760904516418 21.936194884587838 52-53 22.232950314073683 27.642414759814294 27.796635476937986 22.327999449174037 54-55 21.97067634458248 27.59545488709695 27.992172484511396 22.441696283809176 56-57 22.081416337463537 27.735825532529507 27.807615264702278 22.37514286530468 58-59 22.1778750535024 27.628112084318428 27.877119336340723 22.316893525838452 60-61 22.087812237848443 27.694847604348894 27.98640507333604 22.230935084466623 62-63 22.161722905204073 27.590360670540605 27.883667222000884 22.364249202254438 64-65 21.971633645663136 27.464528490891933 27.8922974988835 22.67154036456143 66-67 22.00501111598288 27.614680023249534 27.83835821221538 22.541950648552206 68-69 22.089910886501514 27.513173065525898 27.854002564572266 22.54291348340032 70-71 21.982630038504368 27.399516545626142 27.810171438398847 22.807681977470644 72-73 22.007741240369572 27.512397792012276 27.681549639662812 22.798311327955332 74-75 21.980026048392396 27.717979854045637 27.55339010303545 22.748603994526515 76-77 21.976420525696707 27.516380353024417 27.669871525038776 22.8373275962401 78-79 21.945099552412334 27.442114265376105 27.69059445758783 22.922191724623737 80-81 22.060282852607205 27.540992641846955 27.550690808919875 22.848033696625965 82-83 22.11209667219931 27.50758511166771 27.59481226604152 22.785505950091466 84-85 22.06449020980005 27.552259820031445 27.504529436255186 22.878720533913324 86-87 21.913608675401736 27.62234030755823 27.517315969309408 22.946735047730627 88-89 21.992026342945657 27.388230009175107 27.44517713469611 23.174566513183127 90-91 22.028621942388902 27.42058514687467 27.526210305467135 23.02458260526929 92-93 22.0785281965047 27.218888130801446 27.542450041993476 23.16013363070038 94-95 21.823948434877057 27.257919798315193 27.601571203985575 23.316560562822172 96-97 21.911475515702413 27.001099146592868 27.568208385803345 23.519216951901374 98-99 21.7862938904568 27.033963340224687 27.517974486607987 23.661768282710526 100-101 21.96329166552925 27.07440464316387 27.350192240255172 23.612111451051703 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 27577.0 1 26896.0 2 18638.0 3 12644.0 4 16592.5 5 20120.5 6 20793.0 7 19376.5 8 17552.0 9 15560.0 10 13445.0 11 11550.5 12 9883.0 13 8378.5 14 7156.5 15 6203.5 16 5635.5 17 5405.0 18 5353.0 19 5569.5 20 6008.0 21 6701.0 22 7706.5 23 9084.0 24 10903.0 25 13319.0 26 16314.0 27 20171.0 28 24879.5 29 29813.0 30 35127.0 31 40566.0 32 45608.5 33 49445.0 34 51898.5 35 54042.5 36 56424.0 37 58530.0 38 59591.0 39 59963.0 40 60312.0 41 60817.5 42 61737.5 43 62877.5 44 63787.5 45 64974.5 46 65397.5 47 65072.0 48 64682.0 49 63664.0 50 62151.5 51 60031.0 52 57656.0 53 55131.0 54 51858.0 55 47284.0 56 42067.5 57 36818.5 58 31795.5 59 27007.0 60 22406.5 61 18197.5 62 14531.0 63 11419.0 64 8675.5 65 6528.5 66 4948.5 67 3767.5 68 2865.0 69 2170.5 70 1636.5 71 1227.5 72 912.0 73 663.5 74 490.5 75 357.0 76 227.5 77 147.0 78 96.5 79 60.0 80 37.0 81 24.0 82 14.0 83 8.0 84 5.0 85 6.5 86 5.5 87 1.5 88 1.0 89 1.0 90 1.0 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 13.922541082051065 2 20.630701915513917 3 16.451189012759105 4 21.596748289130975 5 21.420268558160128 6 15.467386338481248 7 14.325896410589726 8 11.521060291354047 9 2.8977623293252135 10-11 6.223654030020865 12-13 3.398518267324168 14-15 7.077557825724499 16-17 10.995129969526985 18-19 13.951083040142429 20-21 14.37386668173833 22-23 12.319999623208474 24-25 14.777763648095554 26-27 12.413184877472105 28-29 12.421780434158036 30-31 13.22528836326471 32-33 11.773487064275924 34-35 18.602786373334464 36-37 23.26687672794239 38-39 22.417588628431748 40-41 17.504603921457807 42-43 17.39328557500742 44-45 22.684663171925262 46-47 12.233996957408428 48-49 20.951822493512122 50-51 20.55237637705528 52-53 10.731705593941191 54-55 12.270687032248645 56-57 11.791667255403427 58-59 12.793838516571762 60-61 12.217771372321836 62-63 12.163160150528213 64-65 13.205271313448161 66-67 11.594016550567778 68-69 12.581540041164473 70-71 11.268327375317329 72-73 10.648034325708016 74-75 10.732506275933854 76-77 10.767689184670237 78-79 11.139865014435825 80-81 11.12644181632355 82-83 9.692137773821468 84-85 9.611810530381172 86-87 9.747950018604081 88-89 11.090575972946368 90-91 8.67835662375953 92-93 10.63830839444421 94-95 10.736462586956419 96-97 10.292555070436466 98-99 10.149162345338853 100-101 10.280850983661377 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2123190.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 97.08575848597684 #Duplication Level Percentage of deduplicated Percentage of total 1 98.77165036601639 95.89320592696413 2 0.8783028604162255 1.705413987678246 3 0.17205574167159446 0.5011248654616213 4 0.06031126145116571 0.2342145825292992 5 0.03412838005868344 0.16566898319474843 6 0.019726432800576007 0.11490934143999444 7 0.012767705696227951 0.08676936741508123 8 0.009324984306397825 0.07242585394051708 9 0.007111657901264255 0.06213966312933274 >10 0.03124465107960474 0.5004874562395137 >50 0.0019164928001392054 0.13297358607717852 >100 0.0012650615992144348 0.21023509748553212 >500 4.860105063091587E-5 0.026895278223191637 >1k 1.4580315189262593E-4 0.2935360102216413 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 3895 0.18345037420108423 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 9.41978814896453E-5 2 0.0 0.0 0.0 0.0 9.41978814896453E-5 3 0.0 0.0 0.0 0.0 9.41978814896453E-5 4 0.0 0.0 0.0 4.709894074482265E-5 9.41978814896453E-5 5 0.0 4.709894074482265E-5 0.0 4.709894074482265E-5 9.41978814896453E-5 6 0.0 4.709894074482265E-5 0.0 4.709894074482265E-5 1.4129682223446794E-4 7 0.0 4.709894074482265E-5 0.0 4.709894074482265E-5 1.4129682223446794E-4 8 0.0 4.709894074482265E-5 0.0 4.709894074482265E-5 1.4129682223446794E-4 9 0.0 4.709894074482265E-5 0.0 4.709894074482265E-5 1.4129682223446794E-4 10-11 0.0 4.709894074482265E-5 0.0 4.709894074482265E-5 1.4129682223446794E-4 12-13 0.0 4.709894074482265E-5 0.0 4.709894074482265E-5 1.883957629792906E-4 14-15 0.0 4.709894074482265E-5 0.0 4.709894074482265E-5 2.119452333517019E-4 16-17 0.0 4.709894074482265E-5 0.0 4.709894074482265E-5 2.3549470372411324E-4 18-19 0.0 7.064841111723397E-5 0.0 4.709894074482265E-5 3.2969258521375856E-4 20-21 0.0 1.1774735186205662E-4 0.0 4.709894074482265E-5 3.767915259585812E-4 22-23 0.0 1.6484629260687928E-4 0.0 1.1774735186205662E-4 3.767915259585812E-4 24-25 0.0 1.883957629792906E-4 0.0 3.767915259585812E-4 3.767915259585812E-4 26-27 0.0 1.883957629792906E-4 0.0 5.180883481930491E-4 4.4743993707581516E-4 28-29 0.0 1.883957629792906E-4 0.0 0.0012481219297378002 5.180883481930491E-4 30-31 0.0 1.883957629792906E-4 0.0 0.004191805726289216 5.180883481930491E-4 32-33 0.0 1.883957629792906E-4 0.0 0.011303745778757436 5.180883481930491E-4 34-35 0.0 2.3549470372411324E-4 0.0 0.021218072805542602 5.180883481930491E-4 36-37 0.0 2.3549470372411324E-4 0.0 0.038126592532933934 5.180883481930491E-4 38-39 0.0 2.3549470372411324E-4 0.0 0.06360711947588299 5.180883481930491E-4 40-41 0.0 2.3549470372411324E-4 0.0 0.09042996623005949 5.180883481930491E-4 42-43 0.0 2.3549470372411324E-4 0.0 0.11866578120658067 5.180883481930491E-4 44-45 0.0 2.3549470372411324E-4 0.0 0.15497906452083893 5.416378185654604E-4 46-47 7.064841111723397E-5 2.3549470372411324E-4 0.0 0.19659097866888975 5.887367593102831E-4 48-49 1.4129682223446794E-4 2.3549470372411324E-4 0.0 0.24248889642471938 6.593851704275171E-4 50-51 1.4129682223446794E-4 2.3549470372411324E-4 0.0 0.29481581959221737 6.593851704275171E-4 52-53 1.4129682223446794E-4 2.3549470372411324E-4 0.0 0.3642867571908308 7.535830519171624E-4 54-55 1.4129682223446794E-4 2.3549470372411324E-4 0.0 0.4407047885493055 7.535830519171624E-4 56-57 1.4129682223446794E-4 2.3549470372411324E-4 0.0 0.5276494331642481 7.535830519171624E-4 58-59 1.4129682223446794E-4 2.3549470372411324E-4 0.0 0.6275462864840169 7.535830519171624E-4 60-61 1.4129682223446794E-4 2.3549470372411324E-4 0.0 0.7272311945704342 7.535830519171624E-4 62-63 1.4129682223446794E-4 2.3549470372411324E-4 0.0 0.836406539216933 7.535830519171624E-4 64-65 1.4129682223446794E-4 2.3549470372411324E-4 0.0 0.9491143044192936 8.477809334068077E-4 66-67 1.4129682223446794E-4 2.3549470372411324E-4 0.0 1.0771056758933493 8.477809334068077E-4 68-69 1.4129682223446794E-4 2.5904417409652457E-4 0.0 1.214281340812645 8.477809334068077E-4 70-71 1.4129682223446794E-4 2.825936444689359E-4 0.0 1.3659634794813464 8.477809334068077E-4 72-73 1.4129682223446794E-4 2.825936444689359E-4 0.0 1.5289493639288052 8.713304037792191E-4 74-75 1.4129682223446794E-4 2.825936444689359E-4 0.0 1.6982700559064428 8.948798741516303E-4 76-77 1.4129682223446794E-4 2.825936444689359E-4 0.0 1.888832370159995 8.948798741516303E-4 78-79 1.4129682223446794E-4 2.825936444689359E-4 0.0 2.100283064633876 8.948798741516303E-4 80-81 1.4129682223446794E-4 2.825936444689359E-4 0.0 2.3281712894277007 9.419788148964529E-4 82-83 1.4129682223446794E-4 2.825936444689359E-4 0.0 2.557189888799401 9.890777556412756E-4 84-85 1.4129682223446794E-4 2.825936444689359E-4 0.0 2.7978419265350722 0.0010361766963860983 86-87 1.4129682223446794E-4 2.825936444689359E-4 0.0 3.0524823496719558 0.0010832756371309208 88-89 1.4129682223446794E-4 2.825936444689359E-4 0.0 3.336465412892864 0.0011539240482481548 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1830 0.0 56.8153 1 GTATCAA 3335 0.0 42.019844 1 ATCAACG 3765 0.0 33.873936 3 TCAACGC 3745 0.0 33.237946 4 TATCAAC 4045 0.0 33.14594 2 CAACGCA 3770 0.0 31.8709 5 AACGCAG 4085 0.0 29.702072 6 GTACATG 4035 0.0 28.879553 1 TACATGG 4205 0.0 26.988579 2 ACGCAGA 4770 0.0 25.356752 7 ACATGGG 4335 0.0 24.902689 3 CGCAGAG 4985 0.0 23.95901 8 GTATTAG 515 0.0 23.407232 1 ATAACGC 195 6.39477E-6 20.599293 3 CATGGGG 3200 0.0 20.23364 4 GCAGAGT 5505 0.0 20.195774 9 GAGTACT 3525 0.0 18.46966 12-13 CAGAGTA 5470 0.0 18.421463 10-11 GTGTAAG 850 0.0 18.318455 1 AGAGTAC 5085 0.0 17.85636 10-11 >>END_MODULE