FastQCFastQC Report
Sat 18 Jun 2016
SRR1930913_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1930913_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2123190
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31250.14718418982757078No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25300.11916032008440129No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA23450.055.074221
GTATCAA44150.040.114411
TCAACGC53950.031.6753044
ATCAACG54200.031.3547743
CAACGCA54300.031.2859765
AACGCAG56700.030.1283786
TATCAAC57450.029.7476672
ATTACGC1004.9763865E-528.4820483
ACGCAGA66800.025.4309677
CGCAGAG68550.024.7125238
GTACATG53900.023.0799471
ACATGGG54150.022.1790033
TACATGG56400.021.631892
GCAGAGT81750.020.6641969
CGTATAC2551.2019882E-820.477293
GGACCGT3052.1100277E-1020.2255256
TATCACG2351.13999704E-720.2009872
AGAGTAC73650.019.55429810-11
ATCACGC2803.5177436E-818.648963
CAGAGTA79100.018.4169710-11
Produced by FastQC (version 0.11.5)