##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1930913_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2123190 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.27674018811317 31.0 31.0 34.0 30.0 34.0 2 31.405318883378314 31.0 31.0 34.0 30.0 34.0 3 31.487316726246828 31.0 31.0 34.0 30.0 34.0 4 35.3536042464405 37.0 35.0 37.0 33.0 37.0 5 35.14126432396535 37.0 35.0 37.0 32.0 37.0 6 35.17761952533687 37.0 35.0 37.0 32.0 37.0 7 35.05673350006359 37.0 35.0 37.0 32.0 37.0 8 35.02998271468875 37.0 35.0 37.0 32.0 37.0 9 36.541720241711765 38.0 35.0 39.0 32.0 39.0 10-11 36.46674108299304 38.0 35.0 39.0 32.0 39.0 12-13 36.36976883839883 38.0 35.0 39.0 32.0 39.0 14-15 37.40419086374747 39.0 36.0 41.0 32.0 41.0 16-17 37.36585515191763 39.0 36.0 41.0 32.0 41.0 18-19 37.34804091955972 39.0 36.0 41.0 32.0 41.0 20-21 37.30677636009966 39.0 36.0 40.0 31.5 41.0 22-23 37.18103843744554 39.0 36.0 40.0 31.0 41.0 24-25 37.07088296384214 39.0 36.0 40.0 31.0 41.0 26-27 36.80572958614161 39.0 36.0 40.0 30.5 41.0 28-29 36.61870605080092 38.5 35.0 40.0 30.0 41.0 30-31 36.377140764604206 38.0 35.0 40.0 30.0 41.0 32-33 36.14358112086059 38.0 35.0 40.0 30.0 41.0 34-35 36.344231321737574 38.0 35.0 40.0 30.0 41.0 36-37 36.40046204060871 38.0 35.0 40.0 30.0 41.0 38-39 36.36544633311197 38.0 35.0 40.0 30.0 41.0 40-41 36.26731969348009 38.0 35.0 40.0 30.0 41.0 42-43 36.12871551768801 38.0 35.0 40.0 30.0 41.0 44-45 35.95113908788191 38.0 35.0 40.0 29.0 41.0 46-47 35.744378976916806 38.0 34.5 40.0 28.0 41.0 48-49 35.554735327502485 38.0 34.0 40.0 28.0 41.0 50-51 35.2811999868123 38.0 34.0 40.0 27.0 41.0 52-53 35.07697992172156 38.0 34.0 40.0 26.5 41.0 54-55 34.91052331633061 38.0 33.0 40.0 26.0 41.0 56-57 34.66305982978443 38.0 33.0 40.0 26.0 41.0 58-59 34.42674419152313 37.0 33.0 40.0 26.0 41.0 60-61 34.11754435542744 37.0 33.0 40.0 25.5 41.0 62-63 33.815094268529904 36.5 32.0 39.5 25.0 41.0 64-65 33.506218944135945 36.0 32.0 39.0 24.0 41.0 66-67 33.1759201955548 36.0 31.5 39.0 23.0 40.0 68-69 32.80829082653931 35.0 31.0 39.0 22.5 40.0 70-71 32.4010119207419 35.0 31.0 38.0 22.0 40.0 72-73 31.978090750239026 35.0 31.0 37.5 21.5 40.0 74-75 31.452880571215953 34.0 30.0 37.0 20.0 39.0 76-77 30.083910766346865 33.5 28.5 35.5 18.5 38.5 78-79 30.850025668922704 34.0 30.0 36.0 20.0 39.0 80-81 30.845113013908318 34.0 30.0 36.0 20.0 37.5 82-83 30.565372152280297 34.0 30.0 35.0 19.0 37.0 84-85 30.237784418728424 34.0 30.0 35.0 18.0 37.0 86-87 29.888719568196912 34.0 29.5 35.0 16.5 36.0 88-89 29.541641586480722 34.0 29.0 35.0 11.5 36.0 90-91 29.202485175608402 34.0 29.0 35.0 7.0 35.5 92-93 28.84033058746509 34.0 29.0 35.0 2.0 35.0 94-95 28.421658212406804 33.0 29.0 35.0 2.0 35.0 96-97 27.964960978527593 33.0 27.5 35.0 2.0 35.0 98-99 27.48105421559069 33.0 27.0 35.0 2.0 35.0 100-101 26.26748030086803 32.0 25.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.44768807243692876 1101 2 0.578238809039334 1101 3 0.47368645686501765 1101 4 0.2480415421738158 1101 5 0.318223219813774 1101 6 0.42246794558337086 1101 7 0.5828545144013475 1101 8 0.5172387459705945 1101 9 0.6687540514210184 1101 10-11 0.6073553522214041 1101 12-13 0.49853614098709187 1101 14-15 0.6820338781068003 1101 16-17 0.5882673721445499 1101 18-19 0.750049395235628 1101 20-21 0.6859882598829756 1101 22-23 0.7494820890474685 1101 24-25 0.5444687933098606 1101 26-27 0.5180401482573629 1101 28-29 0.5234898632017178 1101 30-31 0.5692163419893106 1101 32-33 0.5143075035116595 1101 34-35 0.4649812016735879 1101 36-37 0.6772965036937677 1101 38-39 0.6520993412007172 1101 40-41 0.5616042930898004 1101 42-43 0.580299981870823 1101 44-45 0.7311076534636314 1101 46-47 0.6335892979292339 1101 48-49 0.46052618814110957 1101 50-51 0.4454170828010149 1101 52-53 0.4902018809846638 1101 54-55 0.4258486338347822 1101 56-57 0.4753943984253439 1101 58-59 0.476769842241886 1101 60-61 0.3870960396581964 1101 62-63 0.4860702692900247 1101 64-65 0.4770672100123079 1101 66-67 0.5843859605797519 1101 68-69 0.4197132735552671 1101 70-71 0.4568849882871504 1101 72-73 0.47742725925281704 1101 74-75 0.5041412717426219 1101 76-77 0.6873876224405429 1101 78-79 0.6324204927049948 1101 80-81 0.49710634115447405 1101 82-83 0.6243284609200828 1101 84-85 0.7668205438022788 1101 86-87 0.6286136150647792 1101 88-89 0.6759031377313534 1101 90-91 0.5299771205263504 1101 92-93 0.6922003006116135 1101 94-95 0.6685394635563391 1101 96-97 0.7692999208225508 1101 98-99 1.0187478377363668 1101 100-101 1.2174596990578657 1102 1 0.41001357108314807 1102 2 0.4715947587720848 1102 3 0.4458063613363308 1102 4 0.33238779760080206 1102 5 0.32793272295679543 1102 6 0.3533525018462882 1102 7 0.37735638692088713 1102 8 0.45804171865867715 1102 9 0.5417566601015196 1102 10-11 0.5555754556365144 1102 12-13 0.5877179992899855 1102 14-15 0.8415460585008887 1102 16-17 0.6938331178231252 1102 18-19 0.7308906693675468 1102 20-21 0.6605818921412947 1102 22-23 0.7407509930652907 1102 24-25 0.7660784711619257 1102 26-27 0.7485552866689957 1102 28-29 0.742387417072365 1102 30-31 0.927485295854531 1102 32-33 0.6760391089099258 1102 34-35 0.8073418447617584 1102 36-37 0.7778676819282069 1102 38-39 0.87646177068995 1102 40-41 0.7261899659749602 1102 42-43 0.6733793595969786 1102 44-45 0.7852753619949553 1102 46-47 0.8796103205627901 1102 48-49 0.722690776954785 1102 50-51 0.6260298086391103 1102 52-53 0.7829440998260893 1102 54-55 0.7319733593194897 1102 56-57 0.7003659388031522 1102 58-59 0.6851986319916392 1102 60-61 0.6474594352953673 1102 62-63 0.4560230277356183 1102 64-65 0.616160497999374 1102 66-67 0.7476561484720179 1102 68-69 0.7252096381399511 1102 70-71 0.7773939302214217 1102 72-73 0.6417296530221357 1102 74-75 0.7368427048755741 1102 76-77 0.8623520909720419 1102 78-79 0.8662461265065531 1102 80-81 0.7656410627040735 1102 82-83 0.9550418339232998 1102 84-85 0.9049930733469935 1102 86-87 0.6655232830689499 1102 88-89 0.6996801439776803 1102 90-91 0.7299352973435802 1102 92-93 0.8850497521483938 1102 94-95 0.8523607610395345 1102 96-97 0.8722717781163389 1102 98-99 0.7660631703502254 1102 100-101 1.0066394499910842 1103 1 0.42921643138708276 1103 2 0.5601009236231249 1103 3 0.48919924135844184 1103 4 0.22226980281116937 1103 5 0.3309665984123811 1103 6 0.35063772723543707 1103 7 0.5148066583580544 1103 8 0.4044521161890273 1103 9 0.5512756961591094 1103 10-11 0.5786113536757398 1103 12-13 0.577366561281373 1103 14-15 0.6044460727637286 1103 16-17 0.6117105814871238 1103 18-19 0.795072244194678 1103 20-21 0.7558681441326556 1103 22-23 0.6589689115446973 1103 24-25 0.6462669824033824 1103 26-27 0.7038773408081695 1103 28-29 0.6643021913609033 1103 30-31 0.8113636120657404 1103 32-33 0.9237878766246581 1103 34-35 0.757698093457357 1103 36-37 0.6867410222613444 1103 38-39 0.823958400388733 1103 40-41 0.8414707868054805 1103 42-43 0.8003943887632801 1103 44-45 0.8372797343211786 1103 46-47 0.8563123327514717 1103 48-49 0.8615496046459654 1103 50-51 0.8425429550298489 1103 52-53 0.7105025674006882 1103 54-55 0.8558928685488283 1103 56-57 0.9601875565960896 1103 58-59 0.981771642023169 1103 60-61 0.9564143236221625 1103 62-63 0.8021201262547777 1103 64-65 0.7032752300634542 1103 66-67 0.6082098851348547 1103 68-69 0.6158045839371269 1103 70-71 0.6720690960370135 1103 72-73 0.523550332851979 1103 74-75 0.7717391220016232 1103 76-77 0.9553033064158818 1103 78-79 1.018627859755977 1103 80-81 1.0487598624981054 1103 82-83 0.9249043304683902 1103 84-85 0.8889117203594132 1103 86-87 1.0729612739584589 1103 88-89 0.9907297278975733 1103 90-91 1.00333965210314 1103 92-93 1.0324640683338195 1103 94-95 1.1722825249258584 1103 96-97 1.2527131123124242 1103 98-99 1.3846613103790908 1103 100-101 1.5920104087521345 1104 1 0.4368312024297971 1104 2 0.5546593344098341 1104 3 0.49780993959858577 1104 4 0.2914623109267467 1104 5 0.4587190370112637 1104 6 0.4218230709549289 1104 7 0.4859626443024325 1104 8 0.49788421671821226 1104 9 0.6839700923171605 1104 10-11 0.6072256666900557 1104 12-13 0.5529970567880227 1104 14-15 0.6316892442355027 1104 16-17 0.5979233186012038 1104 18-19 0.6637971669440859 1104 20-21 0.5912153763577592 1104 22-23 0.5657776978401046 1104 24-25 0.6332466325199277 1104 26-27 0.7595036524393777 1104 28-29 0.6405095396748095 1104 30-31 0.6599295946465276 1104 32-33 0.7048193981620017 1104 34-35 0.8293042777433328 1104 36-37 0.8161169693733896 1104 38-39 0.748328670225149 1104 40-41 0.8016577385303876 1104 42-43 0.8375742644009136 1104 44-45 0.9174903371092995 1104 46-47 1.0099692792995185 1104 48-49 0.8692429705345077 1104 50-51 0.8328737284541532 1104 52-53 1.0153630453593934 1104 54-55 0.9672990510293786 1104 56-57 0.9827182753081729 1104 58-59 0.9968164224423219 1104 60-61 1.0504664501573089 1104 62-63 1.0251328541062392 1104 64-65 1.1197314600933908 1104 66-67 1.0379946628144694 1104 68-69 1.120842307500702 1104 70-71 1.050375623392931 1104 72-73 0.9810638823679092 1104 74-75 1.0520391521836139 1104 76-77 1.2015318331461735 1104 78-79 1.270137940501634 1104 80-81 1.0951111973545409 1104 82-83 1.328715523080902 1104 84-85 1.3067861899668536 1104 86-87 1.3082703175268904 1104 88-89 1.2363812055787946 1104 90-91 1.269750962382247 1104 92-93 1.2067504447954782 1104 94-95 1.3386645755507516 1104 96-97 1.3559759464288312 1104 98-99 1.3817816580253854 1104 100-101 1.5742963472020683 1105 1 0.24689296698733898 1105 2 0.3802033629953385 1105 3 0.32986640450878824 1105 4 0.1220582690727241 1105 5 0.21043831864911056 1105 6 0.27627054586307764 1105 7 0.3770771997101292 1105 8 0.39781574030863 1105 9 0.48586027400273224 1105 10-11 0.46061098131297484 1105 12-13 0.42677573249694234 1105 14-15 0.5249571208460608 1105 16-17 0.49915957967284896 1105 18-19 0.4300242331663995 1105 20-21 0.4777294033525905 1105 22-23 0.5837213299219783 1105 24-25 0.5255535115863239 1105 26-27 0.5516599997951843 1105 28-29 0.5185005814861583 1105 30-31 0.48796457387082626 1105 32-33 0.5921116896193297 1105 34-35 0.5020558073084587 1105 36-37 0.49048108928663936 1105 38-39 0.3986920025626972 1105 40-41 0.46167740852452965 1105 42-43 0.35887197965544715 1105 44-45 0.32162326669693186 1105 46-47 0.22239008526491943 1105 48-49 0.45019828434269726 1105 50-51 0.49919478000349926 1105 52-53 0.3452705537104137 1105 54-55 0.48919220067264035 1105 56-57 0.46857304712460035 1105 58-59 0.39381500247824164 1105 60-61 0.5302691100681542 1105 62-63 0.2620287389262046 1105 64-65 0.294344267703778 1105 66-67 0.24153991737988179 1105 68-69 0.26360883204164054 1105 70-71 0.09700175383564158 1105 72-73 0.277478958041133 1105 74-75 0.3970354276876833 1105 76-77 0.4606490888134225 1105 78-79 0.5832442864995464 1105 80-81 0.46444346506820366 1105 82-83 0.5754167253947493 1105 84-85 0.5078626080880788 1105 86-87 0.686807424757454 1105 88-89 0.6648269462685263 1105 90-91 0.6500991057598213 1105 92-93 0.704345332148872 1105 94-95 0.659949196874269 1105 96-97 0.5402447037274847 1105 98-99 0.5503195605910172 1105 100-101 0.8392468036468053 1106 1 -0.15408787822627446 1106 2 0.017247888053681493 1106 3 0.0702412591342032 1106 4 -0.029531031357812765 1106 5 -0.06635566149522276 1106 6 0.025062492688718407 1106 7 0.009096192755251309 1106 8 0.03757982912768654 1106 9 0.0876001478445474 1106 10-11 0.05068449656671703 1106 12-13 0.06815357548468626 1106 14-15 0.12440854840522775 1106 16-17 0.025023547571059623 1106 18-19 0.049247374489745255 1106 20-21 0.08551955562042934 1106 22-23 0.21060561560326363 1106 24-25 0.10260030872427706 1106 26-27 0.17198144129491055 1106 28-29 0.07312349329095724 1106 30-31 0.019681264798684595 1106 32-33 0.0026918786255407667 1106 34-35 -0.11362982159547386 1106 36-37 0.12426132912582233 1106 38-39 -0.00757624146635294 1106 40-41 0.004433325558963475 1106 42-43 0.031267367718939454 1106 44-45 0.026264278118937057 1106 46-47 -0.008177087420833118 1106 48-49 0.014393968161634518 1106 50-51 -0.12279278902535395 1106 52-53 -0.15125277939186077 1106 54-55 -0.10978940703547835 1106 56-57 -0.12261224674386284 1106 58-59 0.06920472189566595 1106 60-61 -0.1759523445249087 1106 62-63 -0.2377777492089237 1106 64-65 -0.0773161603714243 1106 66-67 -0.1240346106822301 1106 68-69 -0.1268076131935416 1106 70-71 -0.09483089017184199 1106 72-73 -0.07015596112674771 1106 74-75 0.16180837376622748 1106 76-77 0.09660031997249163 1106 78-79 0.10899272492618906 1106 80-81 0.1122846043428325 1106 82-83 0.22118391810560567 1106 84-85 0.2235180067802851 1106 86-87 0.07857999343972466 1106 88-89 0.09022128686481068 1106 90-91 0.19496593878892554 1106 92-93 0.3181289608525546 1106 94-95 0.1684180847875716 1106 96-97 0.19874593989283085 1106 98-99 0.3441044601188885 1106 100-101 0.6288697539614283 1107 1 0.07809193427974748 1107 2 0.23203081995289665 1107 3 0.14713703921958654 1107 4 0.07183586367278849 1107 5 0.15372338638166383 1107 6 0.2271259512474657 1107 7 0.24325602609393826 1107 8 0.09787639632679657 1107 9 0.10401275390070452 1107 10-11 0.25343568542047734 1107 12-13 0.236635834834523 1107 14-15 0.2663851812532343 1107 16-17 0.34120664252181854 1107 18-19 0.35708459368088796 1107 20-21 0.35562261765497283 1107 22-23 0.3648040398462413 1107 24-25 0.2289673567112942 1107 26-27 0.5058499308961686 1107 28-29 0.41253646185381854 1107 30-31 0.30847330678216167 1107 32-33 0.4025849620892643 1107 34-35 0.2685375249624755 1107 36-37 0.44346684289878624 1107 38-39 0.3668763657522689 1107 40-41 0.30172515569780245 1107 42-43 0.32688642823002567 1107 44-45 0.5027841295676652 1107 46-47 0.2516601134155323 1107 48-49 0.38545342843961095 1107 50-51 0.36794837659432034 1107 52-53 0.23779703239526384 1107 54-55 0.41728215821343184 1107 56-57 0.40126080860648017 1107 58-59 0.45813293333038274 1107 60-61 0.48464023158238234 1107 62-63 0.2764598165814576 1107 64-65 0.23479599951086527 1107 66-67 0.34899477530482415 1107 68-69 0.2877759782537552 1107 70-71 0.23781547764857436 1107 72-73 0.2715377788259268 1107 74-75 0.4377713873747595 1107 76-77 0.5056686853731378 1107 78-79 0.5163531407229165 1107 80-81 0.536925462673068 1107 82-83 0.5656647151920922 1107 84-85 0.5093797861834801 1107 86-87 0.5849978535488454 1107 88-89 0.656258117240732 1107 90-91 0.7509300154445668 1107 92-93 0.7939715977600201 1107 94-95 0.8009211706833064 1107 96-97 0.8161763179552075 1107 98-99 0.7638738477272788 1107 100-101 0.9708240934114905 1108 1 0.23760799867142524 1108 2 0.24337561920063067 1108 3 0.22819582477247025 1108 4 0.12194284006157119 1108 5 0.19353778390654952 1108 6 0.03428736551899192 1108 7 0.142590230624684 1108 8 0.13051238187406256 1108 9 0.128287895301348 1108 10-11 0.2569504289812272 1108 12-13 0.35583380820894916 1108 14-15 0.3978236472180612 1108 16-17 0.29044743764020353 1108 18-19 0.29848207802038473 1108 20-21 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-0.40728344862669985 2316 68-69 -0.6154587896893773 2316 70-71 -0.4179298902772217 2316 72-73 -0.4670371933934483 2316 74-75 0.032532868062300935 2316 76-77 -0.3921798924953599 2316 78-79 -0.1223238908090778 2316 80-81 -0.439818608823618 2316 82-83 -0.5717562775021925 2316 84-85 -0.5563075970787956 2316 86-87 -0.49907941599019523 2316 88-89 -0.7101988484058666 2316 90-91 -0.23274098710100333 2316 92-93 -0.5252982052930903 2316 94-95 -0.24516757363250008 2316 96-97 -0.5163587329168244 2316 98-99 -0.7588028252179448 2316 100-101 -0.9574767766540013 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4.0 3 16.0 4 32.0 5 85.0 6 155.0 7 401.0 8 911.0 9 1665.0 10 2594.0 11 3962.0 12 5592.0 13 7052.0 14 8359.0 15 9932.0 16 11774.0 17 13384.0 18 12651.0 19 11176.0 20 11406.0 21 12590.0 22 14017.0 23 16085.0 24 19888.0 25 24779.0 26 31774.0 27 40194.0 28 50920.0 29 63385.0 30 77916.0 31 93525.0 32 112484.0 33 135733.0 34 166563.0 35 207823.0 36 267281.0 37 340112.0 38 292409.0 39 54548.0 40 13.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.374249677541535 21.30345767809176 14.714658410724935 23.607634233641768 2 15.41214755139981 21.77257807057662 39.93630102241475 22.87897335560882 3 18.26994860799786 26.526911444764785 30.932512141484146 24.270627805753207 4 12.99163517016591 17.954560231580555 38.51534400209569 30.53846059615785 5 12.841333418741263 38.241259189202424 35.76252953167567 13.154877860380642 6 30.414405130398247 38.63501489269909 17.758483940188057 13.1920960367146 7 26.11089916587776 32.95875545758976 23.37138927745515 17.55895609907733 8 24.704430597355863 36.88553544430786 20.801953664062093 17.60808029427418 9 25.45204407144159 16.14660776784356 21.057569533376444 37.3437786273384 10-11 24.098455625732978 26.83890749297048 29.381967699546436 19.6806691817501 12-13 24.60396855674716 24.36828545726007 29.332301866531026 21.695444119461754 14-15 21.875649818351118 26.042046647272887 26.62171699699909 25.460586537376905 16-17 21.748453977270053 28.945996354541986 27.393262025537048 21.912287642650917 18-19 21.757002435015234 28.003358154475105 29.113880528826908 21.125758881682753 20-21 22.30676482085918 27.250858378195076 29.057267602051628 21.385109198894117 22-23 22.0554656488262 27.183226355838485 28.87283179822041 21.888476197114905 24-25 21.988963235039574 27.344923985358733 28.84053680888047 21.82557597072123 26-27 21.902320320413203 27.464382244451418 28.726102244630404 21.90719519050498 28-29 22.003180593715538 27.599975225939666 28.67510350588278 21.721740674462016 30-31 22.209923256020986 27.287776497115214 28.664462587251354 21.83783765961245 32-33 22.031382312793774 27.488413889435233 28.522454382818047 21.957749414952943 34-35 22.24722991281983 27.424151603759476 28.62185145102048 21.706767032400215 36-37 22.217891694469046 27.358060672970293 28.589992461741748 21.83405517081891 38-39 22.133944934251645 27.388679479891167 28.594399603234972 21.88297598262222 40-41 22.191965609354654 27.33902287442323 28.648618988284074 21.820392527938044 42-43 22.207115271658978 27.438482419544698 28.315208917900097 22.03919339089623 44-45 22.121186857018238 27.460658618693472 28.41606693110142 22.00208759318687 46-47 22.231841672226764 27.533927598670477 28.120263954986623 22.113966774116133 48-49 22.18961612522751 27.56492747943461 28.038223557031046 22.207232838306837 50-51 22.176917940259695 27.65564498432047 27.99939122371113 22.168045851708705 52-53 22.344921213629128 27.614011576099685 27.905416772103138 22.13565043816805 54-55 22.12803693191855 27.544498486686376 28.078715862117697 22.24874871927738 56-57 22.170756063439427 27.674060396519966 27.905928958016364 22.24925458202424 58-59 22.12798452207016 27.634341915930854 28.013917700244185 22.223755861754803 60-61 22.22031630749337 27.722427710198964 28.03153932826245 22.02571665404522 62-63 22.213264766365977 27.634152044798594 28.002114902472165 22.150468286363264 64-65 21.949142753419913 27.577409142234803 28.032446423562078 22.441001680783202 66-67 21.98743622221955 27.662471118328043 27.911488239758015 22.438604419694393 68-69 22.07214003298466 27.582100966248717 27.99028376424927 22.355475236517357 70-71 22.04076196806787 27.44911825402157 27.96132409807104 22.548795679839525 72-73 22.042770278836805 27.61352062278156 27.789652388383505 22.55405670999813 74-75 21.97580892678555 27.68516096375211 27.74416070216063 22.594869407301708 76-77 21.992752852914567 27.573315947739278 27.761699252844647 22.672231946501505 78-79 21.941290679950786 27.59060609429138 27.764955469431513 22.703147756326317 80-81 22.00570327654627 27.65983151101504 27.67243062304846 22.662034589390228 82-83 22.054856136285494 27.54070525953871 27.73237439889977 22.672064205276023 84-85 22.036427279907723 27.597236986438794 27.57359330704897 22.79274242660451 86-87 21.87074637691398 27.712310250142476 27.63527993255432 22.781663440389224 88-89 21.86555443904716 27.511101827371263 27.59484388206123 23.028499851520344 90-91 21.928890781349796 27.476561955381047 27.664193488217858 22.930353775051294 92-93 21.907138609022336 27.38901091136065 27.737138079058788 22.96671240055823 94-95 21.782437733394534 27.291496567479857 27.768076514666777 23.157989184458835 96-97 21.727777542283075 27.144815113108105 27.78872828150095 23.338679063107868 98-99 21.60070460015354 27.20352394274653 27.681907883891693 23.513863573208237 100-101 21.663534587107137 27.17698368963682 27.611730462181903 23.547751261074136 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 146.0 1 114.5 2 130.5 3 174.5 4 162.0 5 168.5 6 241.0 7 453.0 8 703.5 9 819.0 10 789.5 11 742.5 12 787.0 13 889.5 14 1033.0 15 1201.5 16 1367.5 17 1526.5 18 1664.5 19 1905.5 20 2264.0 21 2692.5 22 3339.0 23 4270.5 24 5621.5 25 7696.5 26 10844.5 27 14989.5 28 19786.0 29 26000.5 30 33449.0 31 40495.5 32 47273.5 33 51665.0 34 54421.0 35 57148.0 36 60932.0 37 64726.0 38 65644.0 39 65778.5 40 66259.5 41 66997.0 42 68513.5 43 70597.5 44 71985.5 45 73758.5 46 75408.0 47 76016.0 48 76751.0 49 76726.5 50 75117.5 51 73352.5 52 72321.0 53 71066.0 54 68203.0 55 62446.0 56 55979.0 57 49858.0 58 43454.0 59 37266.0 60 30831.5 61 25171.5 62 20505.5 63 15652.0 64 11568.5 65 8747.0 66 6451.5 67 4850.5 68 3655.0 69 2703.5 70 1982.5 71 1447.5 72 1069.0 73 803.5 74 567.0 75 413.0 76 283.0 77 169.5 78 105.0 79 59.5 80 33.5 81 22.0 82 18.0 83 8.5 84 4.0 85 3.0 86 1.0 87 1.5 88 1.5 89 0.5 90 0.0 91 0.0 92 0.5 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.020535138164742676 2 0.0037208163188409897 3 0.004568597252247797 4 0.03447642462521018 5 0.0021665512742618416 6 9.41978814896453E-5 7 0.0 8 0.0 9 4.709894074482265E-5 10-11 0.0 12-13 0.0 14-15 2.3549470372411324E-5 16-17 0.0 18-19 0.0 20-21 0.0 22-23 9.41978814896453E-5 24-25 6.358357000551057E-4 26-27 0.0026610901520824795 28-29 7.064841111723397E-5 30-31 0.0073945336969371556 32-33 0.02343172302054927 34-35 0.013540945464136513 36-37 9.655282852688643E-4 38-39 8.948798741516304E-4 40-41 0.0025668922705928345 42-43 0.008430710393323253 44-45 0.008053918867364673 46-47 0.008312963041461197 48-49 0.009702381793433466 50-51 0.19710906701708278 52-53 0.049877778248767185 54-55 0.017191113371860266 56-57 0.010361766963860982 58-59 0.020040599286922037 60-61 0.05373989138984265 62-63 0.09664702640837608 64-65 0.10149821730509281 66-67 0.1060432650869682 68-69 0.10380606540158911 70-71 0.09290266061916268 72-73 0.06984772912457199 74-75 0.25358069697012514 76-77 0.3059076201376231 78-79 0.0072532368747026885 80-81 0.001153924048248155 82-83 0.0 84-85 4.709894074482265E-5 86-87 0.0 88-89 1.6484629260687928E-4 90-91 0.006876445348744106 92-93 0.018957323649791115 94-95 0.00195460604091014 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2123190.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 66.0296159273757 #Duplication Level Percentage of deduplicated Percentage of total 1 81.36778187488987 53.72683386061461 2 10.983045500391986 14.504125522075498 3 3.2552695987777374 6.4483260404206915 4 1.4589695660362212 3.853408003804068 5 0.802656106028456 2.649953720141095 6 0.49397151144628426 1.9570049507918055 7 0.3288654815872247 1.5200403008683128 8 0.2456962843265337 1.2978585031091454 9 0.17535339393655314 1.0420665527873174 >10 0.8435510919863776 9.433291719614848 >50 0.03210720733176451 1.4396420737808047 >100 0.011731911540720728 1.4639155694617185 >500 7.860849231628697E-4 0.3193094187300649 >1k 2.1438679722614898E-4 0.34422376380006225 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3125 0.14718418982757078 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2530 0.11916032008440129 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 4.709894074482265E-5 2 0.0 0.0 0.0 0.0 4.709894074482265E-5 3 0.0 0.0 0.0 0.0 4.709894074482265E-5 4 0.0 0.0 0.0 0.0 4.709894074482265E-5 5 0.0 0.0 0.0 0.0 4.709894074482265E-5 6 0.0 0.0 0.0 0.0 1.4129682223446794E-4 7 0.0 0.0 0.0 0.0 1.4129682223446794E-4 8 0.0 0.0 0.0 0.0 1.4129682223446794E-4 9 0.0 0.0 0.0 0.0 1.4129682223446794E-4 10-11 0.0 0.0 0.0 0.0 1.4129682223446794E-4 12-13 0.0 0.0 0.0 0.0 2.825936444689359E-4 14-15 0.0 0.0 0.0 0.0 2.825936444689359E-4 16-17 4.709894074482265E-5 0.0 0.0 2.3549470372411324E-5 3.2969258521375856E-4 18-19 4.709894074482265E-5 0.0 0.0 4.709894074482265E-5 4.2389046670340385E-4 20-21 4.709894074482265E-5 0.0 0.0 4.709894074482265E-5 5.180883481930491E-4 22-23 4.709894074482265E-5 0.0 0.0 9.41978814896453E-5 5.651872889378718E-4 24-25 4.709894074482265E-5 0.0 0.0 4.4743993707581516E-4 5.651872889378718E-4 26-27 4.709894074482265E-5 0.0 0.0 6.829346407999284E-4 5.887367593102831E-4 28-29 4.709894074482265E-5 0.0 0.0 0.002237199685379076 6.122862296826944E-4 30-31 4.709894074482265E-5 0.0 0.0 0.00765357787103368 6.122862296826944E-4 32-33 4.709894074482265E-5 0.0 0.0 0.02168906221299083 6.593851704275171E-4 34-35 4.709894074482265E-5 0.0 0.0 0.04302488237039549 6.593851704275171E-4 36-37 4.709894074482265E-5 0.0 0.0 0.07717161441039191 6.593851704275171E-4 38-39 4.709894074482265E-5 0.0 0.0 0.12688454636655222 7.064841111723397E-4 40-41 4.709894074482265E-5 0.0 0.0 0.17874048012660196 7.064841111723397E-4 42-43 4.709894074482265E-5 0.0 0.0 0.23471757120182368 7.064841111723397E-4 44-45 4.709894074482265E-5 0.0 0.0 0.3041414098596923 7.300335815447511E-4 46-47 4.709894074482265E-5 0.0 0.0 0.3816191673849255 8.477809334068076E-4 48-49 4.709894074482265E-5 0.0 0.0 0.4660440186700201 9.890777556412756E-4 50-51 4.709894074482265E-5 0.0 0.0 0.5543545325665626 0.0010361766963860983 52-53 4.709894074482265E-5 0.0 0.0 0.6435599263372567 0.001083275637130921 54-55 4.709894074482265E-5 0.0 0.0 0.7427738450162256 0.0011303745778757435 56-57 4.709894074482265E-5 0.0 0.0 0.8563058416816205 0.0011303745778757435 58-59 4.709894074482265E-5 0.0 0.0 0.9830961901666833 0.0011303745778757435 60-61 4.709894074482265E-5 0.0 0.0 1.1132776623853728 0.0011539240482481548 62-63 4.709894074482265E-5 0.0 0.0 1.252525680697441 0.0013187703408550342 64-65 4.709894074482265E-5 0.0 0.0 1.398579495947136 0.0013423198112274455 66-67 4.709894074482265E-5 0.0 0.0 1.564438415780029 0.0013658692815998567 68-69 4.709894074482265E-5 0.0 0.0 1.7476532952773893 0.0013658692815998567 70-71 4.709894074482265E-5 0.0 0.0 1.9473763535058097 0.0013894187519722682 72-73 4.709894074482265E-5 0.0 0.0 2.1593686857982566 0.0015071661038343247 74-75 4.709894074482265E-5 0.0 0.0 2.3820995765805226 0.0016249134556963814 76-77 4.709894074482265E-5 0.0 0.0 2.634149557976441 0.0017191113371860267 78-79 4.709894074482265E-5 0.0 0.0 2.9158012236304804 0.0017897597483032606 80-81 4.709894074482265E-5 0.0 0.0 3.215209189945318 0.0018604081594204946 82-83 4.709894074482265E-5 0.0 0.0 3.521211007964431 0.001978155511282551 84-85 4.709894074482265E-5 0.0 0.0 3.8422609375515147 0.002095902863144608 86-87 4.709894074482265E-5 0.0 0.0 4.187708118444416 0.0023078480964963096 88-89 4.709894074482265E-5 0.0 0.0 4.57693376475963 0.0024962438594756005 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2345 0.0 55.07422 1 GTATCAA 4415 0.0 40.11441 1 TCAACGC 5395 0.0 31.675304 4 ATCAACG 5420 0.0 31.354774 3 CAACGCA 5430 0.0 31.285976 5 AACGCAG 5670 0.0 30.128378 6 TATCAAC 5745 0.0 29.747667 2 ATTACGC 100 4.9763865E-5 28.482048 3 ACGCAGA 6680 0.0 25.430967 7 CGCAGAG 6855 0.0 24.712523 8 GTACATG 5390 0.0 23.079947 1 ACATGGG 5415 0.0 22.179003 3 TACATGG 5640 0.0 21.63189 2 GCAGAGT 8175 0.0 20.664196 9 CGTATAC 255 1.2019882E-8 20.47729 3 GGACCGT 305 2.1100277E-10 20.225525 6 TATCACG 235 1.13999704E-7 20.200987 2 AGAGTAC 7365 0.0 19.554298 10-11 ATCACGC 280 3.5177436E-8 18.64896 3 CAGAGTA 7910 0.0 18.41697 10-11 >>END_MODULE