FastQCFastQC Report
Sat 18 Jun 2016
SRR1930912_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1930912_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1900576
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN34820.1832076170592494No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA16200.053.6645771
GTATCAA30300.046.271991
ATCAACG35800.036.9005283
TATCAAC37400.036.1327552
TCAACGC37000.035.683864
CAACGCA37350.034.4890985
AACGCAG39950.032.5190856
ACGCAGA47950.027.2033677
CGCAGAG48450.026.5183968
GTACATG39150.025.8000721
TACATGG40700.024.1925742
GTGGTAT8250.023.7556691
GCAGAGT52500.023.1549959
GTACTAG4550.022.0889571
ACATGGG42550.021.8389643
GAGTACA31700.021.7179051
AGTACAT31750.020.4110982
CATGGGG32300.020.0495684
GTATAGA6150.019.6106851
TTAACGC2351.4895559E-619.2261564
Produced by FastQC (version 0.11.5)