##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1930912_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1900576 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 22.97841338625764 30.0 16.0 31.0 2.0 31.0 2 22.523966418601518 30.0 10.0 31.0 2.0 33.0 3 23.151720320576498 30.0 16.0 31.0 2.0 33.0 4 24.88547629771185 33.0 10.0 35.0 2.0 37.0 5 24.79130168959305 33.0 10.0 35.0 2.0 37.0 6 25.16366880356271 32.0 17.0 35.0 2.0 37.0 7 24.667883841530145 32.0 11.0 35.0 2.0 37.0 8 24.51635241105854 31.0 11.0 35.0 2.0 37.0 9 23.026784511642788 27.0 10.0 34.0 2.0 37.0 10-11 22.95836972580944 27.5 10.0 34.0 2.0 37.0 12-13 22.631443572895797 27.0 10.0 34.0 2.0 37.0 14-15 24.703121843062313 29.5 14.0 35.0 2.0 37.5 16-17 26.643668551007693 32.5 17.0 37.0 2.0 38.5 18-19 27.56986434638762 34.0 18.0 38.0 2.0 39.5 20-21 27.747593098092366 34.5 18.0 38.5 2.0 40.0 22-23 28.153724712929133 35.0 18.5 39.0 2.0 40.0 24-25 28.116829845267958 35.0 18.0 39.0 2.0 40.0 26-27 27.956981462461904 35.0 17.5 39.0 2.0 40.0 28-29 28.09442637389928 35.0 18.5 39.0 2.0 40.0 30-31 28.178571917145117 35.0 19.0 39.0 2.0 40.0 32-33 28.058933449648947 35.0 19.0 39.0 2.0 40.0 34-35 24.693286666778913 32.0 8.5 38.0 2.0 40.0 36-37 22.960367541208562 26.5 8.5 37.5 2.0 40.0 38-39 21.930145650581718 26.5 2.0 37.0 2.0 40.0 40-41 23.783565613792874 30.5 5.5 37.5 2.0 40.0 42-43 23.788353899028504 30.5 5.5 38.0 2.0 40.0 44-45 21.46176580152543 23.5 2.0 37.0 2.0 39.5 46-47 22.764426942148063 27.0 7.5 36.5 2.0 39.0 48-49 22.20701303183877 29.0 2.0 37.0 2.0 39.0 50-51 19.57455029422659 15.5 4.5 35.5 2.0 38.5 52-53 23.284004691209404 28.5 7.0 36.5 2.0 39.0 54-55 25.46888074983584 33.0 6.5 38.0 2.0 39.5 56-57 25.81423947266513 33.0 2.0 38.0 2.0 40.0 58-59 25.427893701698853 33.0 2.0 38.0 2.0 40.0 60-61 25.19033545619854 33.0 2.0 38.0 2.0 40.0 62-63 24.77197991556244 32.0 2.0 37.0 2.0 40.0 64-65 24.310927581954104 31.5 2.0 36.5 2.0 39.0 66-67 23.934267032731128 31.0 2.0 36.0 2.0 39.0 68-69 23.51119187025407 31.0 2.0 36.0 2.0 39.0 70-71 22.928186770747395 30.0 2.0 35.0 2.0 38.0 72-73 22.528164882646102 30.0 2.0 35.0 2.0 37.5 74-75 22.011628316889194 29.0 2.0 34.5 2.0 37.0 76-77 21.492340216860573 29.0 2.0 34.0 2.0 36.0 78-79 21.056141927499873 28.5 2.0 34.0 2.0 36.0 80-81 20.60823613472968 27.5 2.0 34.0 2.0 35.5 82-83 20.19094974365666 27.0 2.0 34.0 2.0 35.0 84-85 19.717028416648425 26.0 2.0 34.0 2.0 35.0 86-87 19.173891230868957 25.5 2.0 33.5 2.0 35.0 88-89 18.65560914164969 25.0 2.0 33.0 2.0 35.0 90-91 18.106552434630345 24.0 2.0 33.0 2.0 35.0 92-93 17.476684699796273 20.5 2.0 32.0 2.0 34.5 94-95 16.81744165979156 18.5 2.0 32.0 2.0 34.0 96-97 16.051531746165374 6.0 2.0 32.0 2.0 34.0 98-99 15.14982510565218 2.0 2.0 31.5 2.0 34.0 100-101 13.67640362711094 2.0 2.0 30.0 2.0 33.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 7.893387504736051 1101 2 8.494704052984929 1101 3 8.10357422534937 1101 4 10.49973719890098 1101 5 10.252098345160778 1101 6 9.686098241625455 1101 7 9.900449169607988 1101 8 9.697055931847949 1101 9 11.449289057382167 1101 10-11 10.873438123883037 1101 12-13 10.18438833370066 1101 14-15 9.026310488774012 1101 16-17 8.202643341133491 1101 18-19 8.001643163665026 1101 20-21 8.660201871762919 1101 22-23 8.393025477179503 1101 24-25 8.55071075652917 1101 26-27 8.654425616363884 1101 28-29 8.531721924018662 1101 30-31 8.196495510166358 1101 32-33 8.19634349961358 1101 34-35 11.249812818757533 1101 36-37 4.354010573485379 1101 38-39 8.036840753047123 1101 40-41 10.02939433592812 1101 42-43 10.415205974867309 1101 44-45 3.949293362553668 1101 46-47 5.559318744310051 1101 48-49 9.87992935402242 1101 50-51 -6.324455690935409 1101 52-53 5.216459683718419 1101 54-55 8.031589023667298 1101 56-57 8.535760065569423 1101 58-59 8.812437010862503 1101 60-61 8.746207585234135 1101 62-63 8.855018093252287 1101 64-65 8.736763436422258 1101 66-67 8.738968141687675 1101 68-69 8.84140020953194 1101 70-71 8.905562035312553 1101 72-73 8.710189669310306 1101 74-75 8.84446275677173 1101 76-77 8.86246829809441 1101 78-79 8.758325457166425 1101 80-81 8.76466464345264 1101 82-83 8.65982163546515 1101 84-85 8.792928928279217 1101 86-87 8.549401026819215 1101 88-89 8.593921051541678 1101 90-91 8.478942751793173 1101 92-93 8.661482745184749 1101 94-95 8.652656722783657 1101 96-97 8.627193354419056 1101 98-99 8.495810899266338 1101 100-101 8.020996607445667 1102 1 7.698428203417365 1102 2 8.234554618313116 1102 3 7.895533452445537 1102 4 10.082813474909784 1102 5 10.000754175339654 1102 6 9.417140325793792 1102 7 9.538131942561307 1102 8 9.23370899105278 1102 9 9.959409050454731 1102 10-11 9.449413036671583 1102 12-13 8.32362990325953 1102 14-15 7.59210528824951 1102 16-17 7.1355375245620785 1102 18-19 7.4200301846205186 1102 20-21 8.08821456381369 1102 22-23 8.004619158369042 1102 24-25 8.091875926375039 1102 26-27 8.264918577594994 1102 28-29 8.176105382293237 1102 30-31 7.9551769471143245 1102 32-33 7.922468341890724 1102 34-35 10.834679341927334 1102 36-37 -0.05903675574097278 1102 38-39 2.5130180459044382 1102 40-41 8.674549213584669 1102 42-43 9.995429260945638 1102 44-45 0.4901531067333842 1102 46-47 0.5437425301533416 1102 48-49 8.13419096366145 1102 50-51 -7.094629742742727 1102 52-53 4.314424625948558 1102 54-55 7.608379889349283 1102 56-57 7.924823872195024 1102 58-59 8.33050821499917 1102 60-61 8.301952903030223 1102 62-63 8.362711875879274 1102 64-65 8.318792061573795 1102 66-67 8.29452963504476 1102 68-69 8.42880254506721 1102 70-71 8.373028944439444 1102 72-73 8.347224727080167 1102 74-75 8.213213220662475 1102 76-77 8.138300332533657 1102 78-79 8.316427587352724 1102 80-81 8.071244840884543 1102 82-83 7.938186142256509 1102 84-85 7.951910224204397 1102 86-87 7.990789606447606 1102 88-89 8.089643731469433 1102 90-91 8.053418493399843 1102 92-93 8.200164305836921 1102 94-95 7.908410823577842 1102 96-97 7.78111836493391 1102 98-99 7.7522056641046575 1102 100-101 7.18401066024262 1103 1 7.560216658539151 1103 2 8.17440780483297 1103 3 7.718990242568992 1103 4 9.788292287055263 1103 5 9.480914335499815 1103 6 8.759442628096092 1103 7 8.945038849468212 1103 8 8.396124740135193 1103 9 8.969912887625235 1103 10-11 7.957991615250162 1103 12-13 7.094754361050654 1103 14-15 6.7811510006285545 1103 16-17 6.791710364068255 1103 18-19 7.301718539642206 1103 20-21 8.030787136386262 1103 22-23 8.149617490034036 1103 24-25 8.295165882998326 1103 26-27 8.543840833183918 1103 28-29 8.413599543120725 1103 30-31 8.176478248415634 1103 32-33 8.047052926475313 1103 34-35 10.893201197115857 1103 36-37 -4.2156166689875505 1103 38-39 -2.42264428309122 1103 40-41 7.430948946651068 1103 42-43 9.896426925276632 1103 44-45 0.21283245274606344 1103 46-47 -0.43745533437785866 1103 48-49 8.50389400003115 1103 50-51 -7.4604055185185025 1103 52-53 4.232005540196141 1103 54-55 7.467395571698294 1103 56-57 8.12088326492108 1103 58-59 8.319593967418257 1103 60-61 8.296460744204733 1103 62-63 8.320736567237294 1103 64-65 8.257624227072629 1103 66-67 8.170615721130847 1103 68-69 8.195512588443918 1103 70-71 8.408327576404744 1103 72-73 8.34241658560536 1103 74-75 8.240090097539355 1103 76-77 8.166498530731857 1103 78-79 8.159991150916287 1103 80-81 8.006840436480136 1103 82-83 8.065173129243497 1103 84-85 8.094763077057252 1103 86-87 8.05796344695478 1103 88-89 7.959306727799099 1103 90-91 8.137025433673447 1103 92-93 8.093520995860274 1103 94-95 8.100903316070333 1103 96-97 8.05645703693925 1103 98-99 7.926843635409295 1103 100-101 7.204657974223267 1104 1 7.04662124494374 1104 2 7.600078930504093 1104 3 7.281816705395457 1104 4 9.346614245377225 1104 5 8.975882030467513 1104 6 8.070408139061602 1104 7 8.129814534243899 1104 8 7.496770840781295 1104 9 7.4350507527631 1104 10-11 6.344955852214397 1104 12-13 5.929361695657988 1104 14-15 6.0607533302308845 1104 16-17 6.649272471630358 1104 18-19 7.249793887717548 1104 20-21 7.9252497808488975 1104 22-23 7.801296441712996 1104 24-25 8.04848283631528 1104 26-27 8.015116654459735 1104 28-29 7.847119426640617 1104 30-31 7.55662202855941 1104 32-33 7.573734153156533 1104 34-35 10.439888793803455 1104 36-37 -4.365643969014851 1104 38-39 -2.729742290189229 1104 40-41 6.7718853375874595 1104 42-43 9.254689733539447 1104 44-45 -0.21606462615101663 1104 46-47 -1.3079121548346784 1104 48-49 7.643215139352289 1104 50-51 -7.620643028756014 1104 52-53 3.6919791501697503 1104 54-55 6.809332963635686 1104 56-57 7.319905013942833 1104 58-59 7.593317691141976 1104 60-61 7.345919293663275 1104 62-63 7.477675324176051 1104 64-65 7.747577817026219 1104 66-67 7.671730472245596 1104 68-69 7.735047577978911 1104 70-71 7.627059945137113 1104 72-73 7.5760092691980425 1104 74-75 7.651114758564017 1104 76-77 7.444198958432285 1104 78-79 7.258717149642283 1104 80-81 7.028134457774154 1104 82-83 7.217143281213648 1104 84-85 7.07241800471218 1104 86-87 6.772669061660395 1104 88-89 7.151435219927588 1104 90-91 7.196662343310358 1104 92-93 7.013763966103248 1104 94-95 6.749406364573382 1104 96-97 6.852029882512099 1104 98-99 6.509922168487826 1104 100-101 6.170077939643228 1105 1 6.488882885741965 1105 2 7.218264344299264 1105 3 6.940760793607836 1105 4 8.947245874789338 1105 5 8.544638547516708 1105 6 7.435676911159646 1105 7 7.615295447529686 1105 8 6.5233807766595255 1105 9 5.962251567768792 1105 10-11 5.4970101459760095 1105 12-13 4.934439900248389 1105 14-15 5.30125012414231 1105 16-17 6.118160228566897 1105 18-19 7.021821813647922 1105 20-21 7.720978791455959 1105 22-23 7.498934123740913 1105 24-25 7.7257723431169865 1105 26-27 7.713154048838593 1105 28-29 7.538195114301672 1105 30-31 7.487543704115232 1105 32-33 7.184085324727217 1105 34-35 9.950412554815017 1105 36-37 -4.741165632585297 1105 38-39 -2.841881080864603 1105 40-41 6.42903581400623 1105 42-43 8.86225250695588 1105 44-45 -4.09832626694924 1105 46-47 -5.502620011249853 1105 48-49 5.911342424064937 1105 50-51 -12.181504178153748 1105 52-53 0.574575183009685 1105 54-55 5.628184165169817 1105 56-57 6.675543534947206 1105 58-59 7.14565124420724 1105 60-61 6.9813085188086 1105 62-63 7.019968726225553 1105 64-65 6.87401036931243 1105 66-67 6.735875615171228 1105 68-69 6.746252108451735 1105 70-71 6.687605879097681 1105 72-73 6.573415633433672 1105 74-75 6.5405664275278745 1105 76-77 6.495256068757687 1105 78-79 6.3913779035225495 1105 80-81 6.349989194994745 1105 82-83 6.393524895644045 1105 84-85 6.4284142892450475 1105 86-87 6.381551425176909 1105 88-89 6.161040900996685 1105 90-91 6.196468779458257 1105 92-93 6.310134194424695 1105 94-95 6.320515611292382 1105 96-97 6.387465850630992 1105 98-99 6.158002110958016 1105 100-101 5.895043481681945 1106 1 6.187951536274031 1106 2 7.271165276590441 1106 3 6.825024848603601 1106 4 8.290884164647139 1106 5 7.777496632082112 1106 6 7.094688588342052 1106 7 6.677199581628944 1106 8 6.211100430110886 1106 9 5.299117091829437 1106 10-11 4.427076771835317 1106 12-13 4.012395216191514 1106 14-15 4.507252101729655 1106 16-17 5.808736318594203 1106 18-19 6.487368475292133 1106 20-21 7.442690110789233 1106 22-23 7.258610411526963 1106 24-25 7.3632160760485235 1106 26-27 7.31198397632706 1106 28-29 7.2851854147066035 1106 30-31 6.840660287597672 1106 32-33 6.840051281973668 1106 34-35 9.83022750914217 1106 36-37 -0.8131606415315247 1106 38-39 0.43793057748364106 1106 40-41 7.344371297573417 1106 42-43 8.877667094016804 1106 44-45 -4.278533913620304 1106 46-47 -5.386422158344683 1106 48-49 5.7869717206088715 1106 50-51 -12.09851684412983 1106 52-53 0.6299749381655388 1106 54-55 5.446490738293463 1106 56-57 6.179289885827703 1106 58-59 6.652149737474026 1106 60-61 6.41026204550603 1106 62-63 6.638661635162361 1106 64-65 6.442282099230503 1106 66-67 6.448867186882314 1106 68-69 6.146240101671431 1106 70-71 6.396378126955295 1106 72-73 6.269406075094846 1106 74-75 6.06386923381849 1106 76-77 5.899964139197465 1106 78-79 5.857536910962047 1106 80-81 5.514642881782581 1106 82-83 5.485307549377918 1106 84-85 5.513708808502983 1106 86-87 5.5820232210145555 1106 88-89 5.6470301655225335 1106 90-91 5.314497548645566 1106 92-93 5.345880498219778 1106 94-95 5.22706519223221 1106 96-97 5.070444238426454 1106 98-99 5.0403231863888465 1106 100-101 4.856568813634105 1107 1 5.699339605015041 1107 2 6.96728156094202 1107 3 6.4527048395188835 1107 4 7.656466343464615 1107 5 7.49466485072092 1107 6 5.985081502264379 1107 7 5.933774644086359 1107 8 5.197677763747045 1107 9 3.542691548639187 1107 10-11 3.224974038850231 1107 12-13 3.1648045583067344 1107 14-15 4.193844576557424 1107 16-17 5.839667880408122 1107 18-19 6.508806509965471 1107 20-21 7.3417929951862355 1107 22-23 7.171338475725616 1107 24-25 7.302068373724346 1107 26-27 7.261354670550691 1107 28-29 7.2681496814944 1107 30-31 7.123025530992322 1107 32-33 6.668786424679393 1107 34-35 9.723724367344907 1107 36-37 -0.9974772059951498 1107 38-39 0.350995533195654 1107 40-41 7.1650484873682565 1107 42-43 8.405670727902791 1107 44-45 -4.462165339163494 1107 46-47 -5.546662335496624 1107 48-49 5.389728363806693 1107 50-51 -12.149362858211138 1107 52-53 0.17177030496090495 1107 54-55 5.011154036044996 1107 56-57 6.306189159050508 1107 58-59 6.601207198296194 1107 60-61 6.667005553720127 1107 62-63 6.5183128199606095 1107 64-65 6.35389917849464 1107 66-67 6.356125495611206 1107 68-69 6.270500075931409 1107 70-71 6.271278762885341 1107 72-73 6.082820224538413 1107 74-75 6.180833046370537 1107 76-77 6.111464820551088 1107 78-79 6.000539787788451 1107 80-81 6.05195834850981 1107 82-83 5.894444312191151 1107 84-85 5.973037675184791 1107 86-87 5.6973763893089 1107 88-89 5.732348887605962 1107 90-91 5.784424303131143 1107 92-93 5.757646150426609 1107 94-95 5.664728906366513 1107 96-97 5.6101416038591125 1107 98-99 5.524720188432909 1107 100-101 5.102623586894758 1108 1 5.642582835179763 1108 2 5.932375051447796 1108 3 6.410098428741346 1108 4 7.485914884974012 1108 5 7.222293424361627 1108 6 6.446281490650652 1108 7 5.956398185111851 1108 8 5.187723347627191 1108 9 2.2509160032779043 1108 10-11 3.020312820957347 1108 12-13 3.250365549731935 1108 14-15 4.390856461271824 1108 16-17 5.410115541209855 1108 18-19 6.674771997838807 1108 20-21 7.88648044074689 1108 22-23 7.885899428438382 1108 24-25 7.590108654002307 1108 26-27 7.79942761443704 1108 28-29 7.828796082206708 1108 30-31 7.419389224544773 1108 32-33 7.398348200031197 1108 34-35 10.200627083021502 1108 36-37 -4.648076522720849 1108 38-39 -2.8828367065116787 1108 40-41 6.391236232950199 1108 42-43 8.7166333607348 1108 44-45 -4.045639246989211 1108 46-47 -5.361618638847187 1108 48-49 6.009110223164452 1108 50-51 -12.246675006170024 1108 52-53 0.6367012032827191 1108 54-55 5.662202174945175 1108 56-57 6.650341647291608 1108 58-59 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88-89 -15.642002763981653 2313 90-91 -15.094645517443386 2313 92-93 -14.522698408422308 2313 94-95 -14.298996583829567 2313 96-97 -13.41358200780202 2313 98-99 -12.338399728099215 2313 100-101 -10.884648577279041 2314 1 -20.444287845965352 2314 2 -19.981247850822687 2314 3 -20.59582457224582 2314 4 -22.233729734966758 2314 5 -22.160727306141826 2314 6 -22.54285967420621 2314 7 -22.054460650031288 2314 8 -21.933204589194133 2314 9 -20.447504529792184 2314 10-11 -20.407624000365455 2314 12-13 -20.10995034365405 2314 14-15 -22.138018168540615 2314 16-17 -24.06940074704286 2314 18-19 -24.97305623513257 2314 20-21 -25.12511876951965 2314 22-23 -25.52155368113713 2314 24-25 -25.488617900785457 2314 26-27 -25.319772780429698 2314 28-29 -25.472536593015406 2314 30-31 -25.550748978811598 2314 32-33 -25.44493906551668 2314 34-35 -22.25519720128254 2314 36-37 -20.45360465697554 2314 38-39 -19.488710349157287 2314 40-41 -21.31754955184743 2314 42-43 -21.345064566214855 2314 44-45 -19.025155535241925 2314 46-47 -20.21798586490839 2314 48-49 -19.779389283252133 2314 50-51 -17.13833344644643 2314 52-53 -20.753229695039096 2314 54-55 -22.84766949336677 2314 56-57 -23.176163782125965 2314 58-59 -22.7877633899391 2314 60-61 -22.55088660314262 2314 62-63 -22.142226395725668 2314 64-65 -21.6733067038583 2314 66-67 -21.29509999458487 2314 68-69 -20.900333257401925 2314 70-71 -20.331662413585246 2314 72-73 -19.94512095193218 2314 74-75 -19.42382381637456 2314 76-77 -18.91132929709597 2314 78-79 -18.47764648672135 2314 80-81 -18.051718122078423 2314 82-83 -17.644036079965712 2314 84-85 -17.174756442462268 2314 86-87 -16.646741257749923 2314 88-89 -16.135788367295998 2314 90-91 -15.596458049810035 2314 92-93 -14.98230482996555 2314 94-95 -14.298996583829567 2314 96-97 -13.538387807905593 2314 98-99 -12.652485693920035 2314 100-101 -11.25129798173269 2315 1 -20.444287845965352 2315 2 -19.981247850822687 2315 3 -20.59582457224582 2315 4 -21.697399363821958 2315 5 -22.160727306141826 2315 6 -22.54285967420621 2315 7 -22.054460650031288 2315 8 -21.933204589194133 2315 9 -19.981743944324332 2315 10-11 -19.813792322372773 2315 12-13 -19.151508106330475 2315 14-15 -21.096199036287608 2315 16-17 -23.568093899159955 2315 18-19 -24.97305623513257 2315 20-21 -25.12511876951965 2315 22-23 -25.52155368113713 2315 24-25 -25.488617900785457 2315 26-27 -25.319772780429698 2315 28-29 -25.472536593015406 2315 30-31 -25.550748978811598 2315 32-33 -25.44493906551668 2315 34-35 -22.25519720128254 2315 36-37 -20.45360465697554 2315 38-39 -19.488710349157287 2315 40-41 -21.31754955184743 2315 42-43 -21.345064566214855 2315 44-45 -19.025155535241925 2315 46-47 -20.21798586490839 2315 48-49 -19.779389283252133 2315 50-51 -17.13833344644643 2315 52-53 -20.753229695039096 2315 54-55 -22.84766949336677 2315 56-57 -23.176163782125965 2315 58-59 -22.7877633899391 2315 60-61 -22.55088660314262 2315 62-63 -22.142226395725668 2315 64-65 -21.6733067038583 2315 66-67 -21.29509999458487 2315 68-69 -20.900333257401925 2315 70-71 -20.331662413585246 2315 72-73 -19.94512095193218 2315 74-75 -19.42382381637456 2315 76-77 -18.91132929709597 2315 78-79 -18.47764648672135 2315 80-81 -18.051718122078423 2315 82-83 -17.644036079965712 2315 84-85 -17.174756442462268 2315 86-87 -16.646741257749923 2315 88-89 -16.135788367295998 2315 90-91 -15.596458049810035 2315 92-93 -14.98230482996555 2315 94-95 -14.298996583829567 2315 96-97 -13.538387807905593 2315 98-99 -12.652485693920035 2315 100-101 -11.25129798173269 2316 1 -20.444287845965352 2316 2 -19.981247850822687 2316 3 -20.59582457224582 2316 4 -22.233729734966758 2316 5 -22.160727306141826 2316 6 -22.54285967420621 2316 7 -22.054460650031288 2316 8 -21.933204589194133 2316 9 -20.447504529792184 2316 10-11 -20.407624000365455 2316 12-13 -20.10995034365405 2316 14-15 -22.138018168540615 2316 16-17 -24.06940074704286 2316 18-19 -24.97305623513257 2316 20-21 -25.12511876951965 2316 22-23 -25.52155368113713 2316 24-25 -25.488617900785457 2316 26-27 -25.319772780429698 2316 28-29 -25.472536593015406 2316 30-31 -25.550748978811598 2316 32-33 -25.44493906551668 2316 34-35 -22.25519720128254 2316 36-37 -20.45360465697554 2316 38-39 -19.488710349157287 2316 40-41 -21.31754955184743 2316 42-43 -21.345064566214855 2316 44-45 -19.025155535241925 2316 46-47 -20.21798586490839 2316 48-49 -19.779389283252133 2316 50-51 -17.13833344644643 2316 52-53 -20.753229695039096 2316 54-55 -22.84766949336677 2316 56-57 -23.176163782125965 2316 58-59 -22.7877633899391 2316 60-61 -22.55088660314262 2316 62-63 -22.142226395725668 2316 64-65 -21.6733067038583 2316 66-67 -21.29509999458487 2316 68-69 -20.900333257401925 2316 70-71 -20.331662413585246 2316 72-73 -19.94512095193218 2316 74-75 -19.42382381637456 2316 76-77 -18.91132929709597 2316 78-79 -18.47764648672135 2316 80-81 -18.051718122078423 2316 82-83 -17.644036079965712 2316 84-85 -17.174756442462268 2316 86-87 -16.646741257749923 2316 88-89 -16.135788367295998 2316 90-91 -15.596458049810035 2316 92-93 -14.98230482996555 2316 94-95 -14.298996583829567 2316 96-97 -13.538387807905593 2316 98-99 -12.652485693920035 2316 100-101 -11.25129798173269 >>END_MODULE >>Per sequence quality scores fail #Quality Count 2 299906.0 3 6933.0 4 8157.0 5 5925.0 6 7223.0 7 11429.0 8 16923.0 9 20474.0 10 20911.0 11 20812.0 12 20457.0 13 20249.0 14 21987.0 15 24246.0 16 26734.0 17 28326.0 18 29454.0 19 31796.0 20 34228.0 21 37578.0 22 41211.0 23 45411.0 24 50374.0 25 55620.0 26 62156.0 27 69504.0 28 77975.0 29 86839.0 30 95010.0 31 97801.0 32 99549.0 33 102771.0 34 103135.0 35 95861.0 36 77273.0 37 39820.0 38 6464.0 39 54.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.51357665232444 19.956185231637296 14.362654568935868 24.167583547102403 2 15.092931678681582 20.079882939087767 40.430813846376104 24.396371535854545 3 18.399327887628854 25.441907590842156 29.850410945110823 26.30835357641817 4 12.104832742185545 16.401617332046158 39.58310831254197 31.910441613226325 5 12.905049650761875 37.42371393453098 36.18547257716259 13.485763837544553 6 30.55298705288103 38.12824245393951 17.653002785175826 13.665767708003635 7 26.113144709732598 34.23092545166955 22.1554604679472 17.500469370650652 8 24.330243482466518 38.23694903914768 19.884649000586442 17.548158477799358 9 24.79383073969587 20.051179379173764 19.87469502550302 35.280294855627346 10-11 23.614339590898258 30.255723639014683 27.103817628667965 19.026119141419098 12-13 23.996997649411046 27.596796403713242 27.428492710016165 20.977713236859543 14-15 21.840893188731417 27.25704243981923 25.670603127609184 25.231461243840176 16-17 21.790092552800285 29.24337679637646 26.826577584411787 22.139953066411472 18-19 21.734080418703446 28.01524313217405 28.888753790849293 21.36192265827321 20-21 22.467117491710876 27.080230956509784 28.836932167192668 21.615719384586672 22-23 22.30854469564008 26.834443336473363 28.682812930042516 22.17419903784404 24-25 22.15072693161424 27.013777450577937 28.75690823288336 22.078587384924468 26-27 22.13403144252755 27.17016051428925 28.51276208359421 22.183045959588988 28-29 22.093758329791296 27.266723819541 28.62377439503202 22.01574345563568 30-31 22.340002786510865 27.03472840605521 28.572246351790938 22.05302245564299 32-33 22.235607105228116 27.163249797960752 28.39937551775816 22.201767579052976 34-35 22.59111154878736 27.12926176035181 28.39419275369572 21.885433937165118 36-37 23.014416946417885 27.093100657021584 27.98118236492159 21.911300031638937 38-39 22.749676782844148 27.183782494504634 28.057756263094703 22.008784459556523 40-41 22.710256589116916 26.990319897895642 28.248245837836393 22.05117767515105 42-43 22.700156496414646 27.188826175083186 27.909778655721567 22.201238672780597 44-45 23.078010891059154 27.28730769060798 27.625379927585573 22.00930149074729 46-47 22.477904456234057 27.284882815774665 27.880234967245904 22.35697776074538 48-49 22.989123243971378 27.413195056225497 27.3484358388413 22.249245860961828 50-51 23.276509790122837 27.493002330792653 27.084022297299086 22.146465581785424 52-53 22.34995982446381 27.47194334807908 27.69504266259319 22.48305416486392 54-55 22.139844531993617 27.384748273492864 27.833139951755438 22.642267242758074 56-57 22.26693568233429 27.5303789676782 27.677231251977545 22.525454098009973 58-59 22.358010443754086 27.431487346063104 27.800815372145372 22.409686838037445 60-61 22.333970717829843 27.538811806739595 27.786840983543748 22.34037649188682 62-63 22.265712298337334 27.442607011166707 27.682694968624023 22.60898572187194 64-65 22.037748530840986 27.3503365214311 27.759369767871362 22.852545179856552 66-67 22.09298559296524 27.408816809306703 27.68543972167141 22.81275787605665 68-69 22.16127983226667 27.35449716366924 27.70542434357442 22.778798660489674 70-71 22.106322936746945 27.181343590745826 27.68334680655124 23.02898666595599 72-73 22.233709684960253 27.30435611056075 27.56438247487583 22.897551729603162 74-75 22.187171058053913 27.4589256741323 27.458866803090494 22.895036464723294 76-77 22.13004613746041 27.361952854019002 27.48698702118836 23.02101398733223 78-79 22.063208197910296 27.27010817673165 27.58604268147673 23.080640943881324 80-81 22.075982931456174 27.452036114540906 27.532547555858322 22.9394333981446 82-83 22.260857670075847 27.28923517530022 27.587388163524285 22.862518991099652 84-85 22.231806597577762 27.36358545958194 27.387192677343407 23.017415265496886 86-87 22.000354659388677 27.411501854391062 27.52893545985317 23.05920802636709 88-89 22.107135489111062 27.208748036704538 27.350028462981278 23.334088011203125 90-91 22.07379515645181 27.272442103275925 27.40600343961451 23.247759300657762 92-93 22.170918483606012 27.097184877223363 27.444484790927625 23.287411848242993 94-95 21.88865586542051 27.075249963790434 27.54406086504958 23.492033305739483 96-97 21.938835006642254 26.86139868819144 27.486889825309007 23.712876479857297 98-99 21.81482431452673 26.95589942346281 27.464613870994064 23.764662391016397 100-101 22.07161775438079 26.95871711701581 27.224334667571 23.745330461032413 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 25085.0 1 24107.0 2 16344.5 3 11011.0 4 14795.5 5 17985.5 6 18620.0 7 17378.0 8 15594.0 9 13810.5 10 12034.5 11 10522.0 12 8981.0 13 7634.0 14 6630.0 15 5834.0 16 5328.5 17 5160.5 18 5219.5 19 5412.5 20 5752.5 21 6402.5 22 7216.0 23 8384.5 24 10013.0 25 11917.0 26 14319.5 27 17035.0 28 20155.0 29 23961.5 30 27884.5 31 31712.0 32 35580.0 33 38848.0 34 41445.0 35 44022.0 36 46754.5 37 49882.0 38 52371.0 39 53537.5 40 54308.0 41 55181.0 42 56295.5 43 57594.0 44 58867.5 45 60335.0 46 61085.0 47 60989.5 48 60631.5 49 59398.5 50 57486.5 51 55610.5 52 53622.5 53 51178.0 54 48241.0 55 44033.5 56 39216.0 57 34493.5 58 30042.5 59 25797.5 60 21426.0 61 17409.0 62 13839.5 63 10685.0 64 8069.5 65 5972.0 66 4379.0 67 3271.0 68 2448.5 69 1772.0 70 1310.5 71 992.0 72 753.0 73 556.5 74 396.5 75 284.5 76 181.0 77 117.5 78 73.0 79 40.5 80 21.5 81 10.0 82 7.0 83 5.5 84 3.5 85 3.0 86 1.0 87 0.0 88 0.0 89 0.5 90 1.5 91 1.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 13.87395189668816 2 20.605227046958397 3 16.392714629670163 4 21.570986900813228 5 21.363891788594618 6 15.385809354637752 7 14.244839459195527 8 11.445951122186116 9 2.8686040442476384 10-11 6.1771273550755135 12-13 3.3681894330981765 14-15 7.017846168740424 16-17 10.931343971511795 18-19 13.88550102705706 20-21 14.30011217651912 22-23 12.252233007256748 24-25 14.701464187698887 26-27 12.351150388092872 28-29 12.357727341605914 30-31 13.141594969104103 32-33 11.68411576280033 34-35 18.520359091138687 36-37 23.169291835738218 38-39 22.35048743117876 40-41 17.40574962537673 42-43 17.309199947805297 44-45 22.610855866852997 46-47 12.130848753220077 48-49 20.864359015372184 50-51 20.44874816897614 52-53 10.61683405451821 54-55 12.162891670735608 56-57 11.699558449648949 58-59 12.691468270671628 60-61 12.112538514639773 62-63 12.065473835300457 64-65 13.107657888976815 66-67 11.468128609432087 68-69 12.469009395046553 70-71 11.156670398868554 72-73 10.532149201084302 74-75 10.62562086441163 76-77 10.68307713030155 78-79 11.05948933375987 80-81 11.023605475392722 82-83 9.611086323304093 84-85 9.510958783021568 86-87 9.65157404913037 88-89 10.9916677891334 90-91 8.585397269038438 92-93 10.524625166265384 94-95 10.635196908726616 96-97 10.211483255602511 98-99 10.048138038152645 100-101 10.167128281110568 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1900576.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 96.21756559496252 #Duplication Level Percentage of deduplicated Percentage of total 1 98.27897191360327 94.56163426702604 2 1.238493469779318 2.383296533348485 3 0.2454838582123353 0.7085957769014957 4 0.09551635959635796 0.367614063794184 5 0.04162984993698949 0.20027614085103668 6 0.02387056152682785 0.13780603916976888 7 0.016178021492924237 0.10896268909345062 8 0.011742265577760647 0.09038497667693245 9 0.006902492893143833 0.05977269564433466 >10 0.037066774979063 0.6196632830949665 >50 0.0024372178504282276 0.15974693282354652 >100 0.0014870804732711438 0.2335953846848239 >500 5.503351957547064E-5 0.04109068433027412 >1k 1.651005587264119E-4 0.32756053256066464 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 3482 0.1832076170592494 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 5.2615628104322056E-5 5 0.0 0.0 0.0 0.0 5.2615628104322056E-5 6 0.0 0.0 0.0 0.0 5.2615628104322056E-5 7 0.0 0.0 0.0 0.0 5.2615628104322056E-5 8 5.2615628104322056E-5 0.0 0.0 0.0 1.0523125620864411E-4 9 5.2615628104322056E-5 0.0 0.0 0.0 1.5784688431296618E-4 10-11 5.2615628104322056E-5 0.0 0.0 0.0 1.5784688431296618E-4 12-13 5.2615628104322056E-5 0.0 0.0 0.0 3.1569376862593235E-4 14-15 5.2615628104322056E-5 0.0 0.0 0.0 3.6830939673025443E-4 16-17 5.2615628104322056E-5 0.0 0.0 0.0 3.6830939673025443E-4 18-19 5.2615628104322056E-5 0.0 0.0 0.0 3.6830939673025443E-4 20-21 5.2615628104322056E-5 0.0 0.0 0.0 5.787719091475427E-4 22-23 5.2615628104322056E-5 0.0 0.0 2.6307814052161028E-5 6.840031653561867E-4 24-25 5.2615628104322056E-5 0.0 0.0 5.2615628104322056E-5 7.366187934605089E-4 26-27 5.2615628104322056E-5 0.0 0.0 1.3153907026080514E-4 8.418500496691529E-4 28-29 5.2615628104322056E-5 0.0 0.0 9.99696933982119E-4 8.418500496691529E-4 30-31 5.2615628104322056E-5 0.0 0.0 0.0035778627110939 8.418500496691529E-4 32-33 5.2615628104322056E-5 0.0 0.0 0.011443899112690049 8.418500496691529E-4 34-35 5.2615628104322056E-5 0.0 0.0 0.024597806138770564 8.94465677773475E-4 36-37 5.2615628104322056E-5 0.0 0.0 0.04472328388867375 8.94465677773475E-4 38-39 5.2615628104322056E-5 0.0 0.0 0.07132048389540854 8.94465677773475E-4 40-41 5.2615628104322056E-5 0.0 0.0 0.0986279948815517 8.94465677773475E-4 42-43 5.2615628104322056E-5 0.0 0.0 0.12685627935952049 8.94465677773475E-4 44-45 5.2615628104322056E-5 0.0 0.0 0.1626875220985638 8.94465677773475E-4 46-47 5.2615628104322056E-5 0.0 0.0 0.20735819035913322 9.20773491825636E-4 48-49 5.2615628104322056E-5 0.0 0.0 0.25181839610728535 0.001052312562086441 50-51 5.2615628104322056E-5 0.0 0.0 0.3044603320256596 0.0011049281901907631 52-53 5.2615628104322056E-5 0.0 0.0 0.37307111107369556 0.0012101594463994074 54-55 5.2615628104322056E-5 0.0 0.0 0.44996885154816224 0.0013680063307123735 56-57 5.2615628104322056E-5 0.0 0.0 0.5430722054787601 0.0014732375869210177 58-59 5.2615628104322056E-5 0.0 0.0 0.6421211253851464 0.0014732375869210177 60-61 5.2615628104322056E-5 0.0 0.0 0.7475365362921556 0.0014732375869210177 62-63 5.2615628104322056E-5 0.0 0.0 0.8530571784553735 0.0016310844712339838 64-65 5.2615628104322056E-5 0.0 0.0 0.9693377165659254 0.0016310844712339838 66-67 5.2615628104322056E-5 0.0 0.0 1.0971147694172714 0.0016310844712339838 68-69 5.2615628104322056E-5 0.0 0.0 1.2441491421547992 0.0016310844712339838 70-71 5.2615628104322056E-5 0.0 0.0 1.399628323203071 0.0016837000993383058 72-73 5.2615628104322056E-5 0.0 0.0 1.5674458690418063 0.00178893135554695 74-75 5.2615628104322056E-5 0.0 0.0 1.7361841883723672 0.001841546983651272 76-77 5.2615628104322056E-5 0.0 0.0 1.9242324432172142 0.001841546983651272 78-79 5.2615628104322056E-5 0.0 0.0 2.1397723637465695 0.001841546983651272 80-81 5.2615628104322056E-5 0.0 0.0 2.367887419392858 0.0018678547977034332 82-83 5.2615628104322056E-5 0.0 0.0 2.6161805684171533 0.0019467782398599161 84-85 5.2615628104322056E-5 0.0 0.0 2.866525726937518 0.001999393867964238 86-87 5.2615628104322056E-5 0.0 0.0 3.1293407893186065 0.0020257016820163995 88-89 5.2615628104322056E-5 0.0 0.0 3.4163327328136313 0.0020520094960685604 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1620 0.0 53.664577 1 GTATCAA 3030 0.0 46.27199 1 ATCAACG 3580 0.0 36.900528 3 TATCAAC 3740 0.0 36.132755 2 TCAACGC 3700 0.0 35.68386 4 CAACGCA 3735 0.0 34.489098 5 AACGCAG 3995 0.0 32.519085 6 ACGCAGA 4795 0.0 27.203367 7 CGCAGAG 4845 0.0 26.518396 8 GTACATG 3915 0.0 25.800072 1 TACATGG 4070 0.0 24.192574 2 GTGGTAT 825 0.0 23.755669 1 GCAGAGT 5250 0.0 23.154995 9 GTACTAG 455 0.0 22.088957 1 ACATGGG 4255 0.0 21.838964 3 GAGTACA 3170 0.0 21.717905 1 AGTACAT 3175 0.0 20.411098 2 CATGGGG 3230 0.0 20.049568 4 GTATAGA 615 0.0 19.610685 1 TTAACGC 235 1.4895559E-6 19.226156 4 >>END_MODULE