FastQCFastQC Report
Sat 18 Jun 2016
SRR1930912_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1930912_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1900576
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39600.20835788729311536No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30920.1626875220985638No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21350.11233436600272759No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA22400.056.6103481
GTATCAA42550.041.521781
ATCAACG51850.033.7905963
CAACGCA52850.033.3098645
TCAACGC53750.032.6836134
TATCAAC55550.031.6270542
AACGCAG56050.031.5766226
ACGCAGA64600.027.3973667
CGCAGAG66600.026.5033768
GTACATG47250.024.3246231
GCAGAGT72950.023.6760149
TACATGG49300.022.5377222
ACATGGG49750.021.8554953
AGAGTAC70100.020.27268810-11
CAGAGTA71900.019.46819310-11
CATGGGG35550.019.0986754
GTGGTAT11000.018.997331
GTCCTAC5550.018.8261831
GAGTACT52550.018.42001312-13
TATACTG10300.017.9668225
Produced by FastQC (version 0.11.5)