##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1930912_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1900576 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.23165187816746 31.0 31.0 33.0 30.0 34.0 2 31.35338760459987 31.0 31.0 34.0 30.0 34.0 3 31.43624406495715 31.0 31.0 34.0 28.0 34.0 4 35.30515380600407 37.0 35.0 37.0 33.0 37.0 5 35.09340799841732 35.0 35.0 37.0 32.0 37.0 6 35.13096292913306 37.0 35.0 37.0 32.0 37.0 7 35.00241558348627 36.0 35.0 37.0 32.0 37.0 8 34.98379333423131 36.0 35.0 37.0 32.0 37.0 9 36.485758527941 38.0 35.0 39.0 32.0 39.0 10-11 36.403226179852844 38.0 35.0 39.0 32.0 39.0 12-13 36.30033158368832 38.0 35.0 39.0 32.0 39.0 14-15 37.3194292151432 39.0 36.0 41.0 32.0 41.0 16-17 37.28022083831428 39.0 36.0 40.5 32.0 41.0 18-19 37.27285701808294 39.0 36.0 40.0 31.5 41.0 20-21 37.232090166349565 39.0 36.0 40.0 31.0 41.0 22-23 37.10587264071523 39.0 36.0 40.0 31.0 41.0 24-25 36.984563890104894 39.0 36.0 40.0 31.0 41.0 26-27 36.71311065697978 38.5 35.5 40.0 30.0 41.0 28-29 36.51908658217298 38.0 35.0 40.0 30.0 41.0 30-31 36.27177524076912 38.0 35.0 40.0 30.0 41.0 32-33 36.023060377485564 38.0 34.5 40.0 29.5 41.0 34-35 36.21439105828969 38.0 35.0 40.0 30.0 41.0 36-37 36.2658788704056 38.0 35.0 40.0 30.0 41.0 38-39 36.22241073232536 38.0 35.0 40.0 30.0 41.0 40-41 36.116684889212536 38.0 35.0 40.0 30.0 41.0 42-43 35.96401275192363 38.0 35.0 40.0 29.0 41.0 44-45 35.76902817882579 38.0 34.5 40.0 28.5 41.0 46-47 35.529021728149786 38.0 34.0 40.0 27.5 41.0 48-49 35.31103465475729 38.0 34.0 40.0 27.0 41.0 50-51 35.02680529481589 38.0 33.5 40.0 26.5 41.0 52-53 34.80819314776152 38.0 33.0 40.0 26.0 41.0 54-55 34.627626572154966 38.0 33.0 40.0 26.0 41.0 56-57 34.36512483583924 37.0 33.0 40.0 25.5 41.0 58-59 34.115065906335765 37.0 33.0 40.0 25.0 41.0 60-61 33.79577007180981 36.5 32.0 39.5 24.0 41.0 62-63 33.4775223405957 36.0 32.0 39.0 23.0 41.0 64-65 33.16301794824306 36.0 31.5 39.0 23.0 40.5 66-67 32.82097295767178 35.0 31.0 39.0 22.0 40.0 68-69 32.44507612429074 35.0 31.0 38.0 22.0 40.0 70-71 32.04598369125991 35.0 31.0 38.0 21.5 40.0 72-73 31.623942688953242 34.5 30.0 37.0 20.0 39.5 74-75 31.098236008452176 34.0 30.0 36.5 18.5 39.0 76-77 29.7412652795784 32.5 28.5 35.0 18.0 38.0 78-79 30.509184320963747 34.0 29.5 36.0 18.0 38.0 80-81 30.51600356944421 34.0 30.0 35.5 18.0 37.0 82-83 30.243888431717544 34.0 30.0 35.0 18.0 37.0 84-85 29.929272494233327 34.0 29.0 35.0 16.0 36.5 86-87 29.594544232906237 34.0 29.0 35.0 12.0 36.0 88-89 29.244522186958058 34.0 29.0 35.0 7.5 36.0 90-91 28.90732940961056 34.0 29.0 35.0 4.5 35.0 92-93 28.54822169700133 33.0 29.0 35.0 2.0 35.0 94-95 28.121907779536308 33.0 27.5 35.0 2.0 35.0 96-97 27.656958206354282 33.0 27.0 35.0 2.0 35.0 98-99 27.15725390618423 33.0 26.5 35.0 2.0 35.0 100-101 25.93353409703164 32.0 24.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.49679014894643814 1101 2 0.5326910429099101 1101 3 0.5468222675964611 1101 4 0.36955476603139914 1101 5 0.441464092764015 1101 6 0.329150103458538 1101 7 0.44607380096790905 1101 8 0.4777763849830947 1101 9 0.6621247866828526 1101 10-11 0.6462310829814726 1101 12-13 0.6680354453469661 1101 14-15 0.8327931651176215 1101 16-17 0.679223770081137 1101 18-19 0.7615484238869286 1101 20-21 0.7007612269753523 1101 22-23 0.9147579943262514 1101 24-25 0.8427220803948998 1101 26-27 0.8149696765478325 1101 28-29 0.6293383524934839 1101 30-31 0.7480961792819016 1101 32-33 0.604335875065388 1101 34-35 0.6977561980720353 1101 36-37 0.6892668173872849 1101 38-39 0.7096409716782404 1101 40-41 0.7351418350344545 1101 42-43 0.5814864532067574 1101 44-45 0.5427044681143727 1101 46-47 0.6846970379341641 1101 48-49 0.8325206660399544 1101 50-51 0.7122481475100884 1101 52-53 0.5790195338869211 1101 54-55 0.6309105252090106 1101 56-57 0.8218561868489616 1101 58-59 0.6856819774486809 1101 60-61 0.6468742506385823 1101 62-63 0.6468838246594473 1101 64-65 0.7249296435967238 1101 66-67 0.6655113113425344 1101 68-69 0.5564920440314438 1101 70-71 0.4857532485634479 1101 72-73 0.5879239380977275 1101 74-75 0.5939634523271273 1101 76-77 0.6928923403198475 1101 78-79 0.7764240744033764 1101 80-81 0.5993240510679456 1101 82-83 0.8961529230055589 1101 84-85 0.9389140363614388 1101 86-87 0.7016315150950696 1101 88-89 0.8012255852284014 1101 90-91 0.6205770507677677 1101 92-93 0.7444941905787736 1101 94-95 0.6978164264723681 1101 96-97 0.7851585968456511 1101 98-99 0.9419905014507854 1101 100-101 1.013252601264444 1102 1 0.397283728698536 1102 2 0.5257106623957952 1102 3 0.40462998179028276 1102 4 0.29747554586266745 1102 5 0.36653448045297665 1102 6 0.3155077570373024 1102 7 0.324906280742276 1102 8 0.4354633638791583 1102 9 0.5798085492697567 1102 10-11 0.6001969158818454 1102 12-13 0.5070281220584079 1102 14-15 0.563664413787798 1102 16-17 0.6683656340559949 1102 18-19 0.7613445593677639 1102 20-21 0.6774014209435322 1102 22-23 0.7313628088936568 1102 24-25 0.7644830096608359 1102 26-27 0.7165976238003609 1102 28-29 0.7126335849336698 1102 30-31 0.7071443991784889 1102 32-33 0.6977659450819615 1102 34-35 0.8019730267911953 1102 36-37 0.826807949033288 1102 38-39 0.7448385272832851 1102 40-41 0.7904638370632 1102 42-43 0.7971023764111962 1102 44-45 0.8142349364584689 1102 46-47 0.7319296513090663 1102 48-49 0.7709493753103374 1102 50-51 0.89539679047504 1102 52-53 1.045447822563041 1102 54-55 0.9145912199317934 1102 56-57 0.6873215620437989 1102 58-59 0.8598896520571557 1102 60-61 0.849647203952621 1102 62-63 0.6491561470820741 1102 64-65 0.722756037124519 1102 66-67 0.7439204753495545 1102 68-69 0.7473754157130799 1102 70-71 0.7945596267519264 1102 72-73 0.8553101920871846 1102 74-75 0.943458368084567 1102 76-77 1.0382127341940155 1102 78-79 1.023181911064352 1102 80-81 0.9297229971081755 1102 82-83 1.1202241518830647 1102 84-85 1.0961096621809787 1102 86-87 1.027045924160113 1102 88-89 1.173548502668222 1102 90-91 1.0413536657260778 1102 92-93 1.2481025183454157 1102 94-95 1.2003288587443173 1102 96-97 1.2570536214627843 1102 98-99 1.3911172735134336 1102 100-101 1.8826217098519926 1103 1 0.4355219669367756 1103 2 0.5123039223223884 1103 3 0.45827028876392006 1103 4 0.31935075440453886 1103 5 0.3243389515907822 1103 6 0.4598454280416391 1103 7 0.531733107569103 1103 8 0.5263876214700787 1103 9 0.4878232306177708 1103 10-11 0.5576176699692681 1103 12-13 0.5830007613643886 1103 14-15 0.6988062222629381 1103 16-17 0.6262401752638738 1103 18-19 0.5493392206957566 1103 20-21 0.6022729591484008 1103 22-23 0.7003150945126038 1103 24-25 0.6109494761273027 1103 26-27 0.7123600528961234 1103 28-29 0.7358301147968689 1103 30-31 0.8156662543670095 1103 32-33 0.9320669127162731 1103 34-35 0.8305282486797481 1103 36-37 0.824488963380972 1103 38-39 0.8834104325218561 1103 40-41 0.9462763162090226 1103 42-43 0.9305725132146634 1103 44-45 1.0439246261481614 1103 46-47 0.9445222439016518 1103 48-49 0.985063489424455 1103 50-51 0.8694441711890804 1103 52-53 0.8166456870942298 1103 54-55 0.777063692404262 1103 56-57 0.8925067472289854 1103 58-59 0.8598396020071064 1103 60-61 0.8108784351838523 1103 62-63 0.7981851761110974 1103 64-65 0.7691324135008983 1103 66-67 0.6415314196271638 1103 68-69 0.69491628992062 1103 70-71 0.6871989327245629 1103 72-73 0.6888337156107092 1103 74-75 0.7206755853017839 1103 76-77 0.8296074622554102 1103 78-79 0.9060514472672203 1103 80-81 0.693316590701766 1103 82-83 0.8767540150795945 1103 84-85 0.8899968827348665 1103 86-87 0.8164519968995201 1103 88-89 0.80043872622511 1103 90-91 0.8230320107377551 1103 92-93 0.8057902060331052 1103 94-95 0.8239024323178903 1103 96-97 0.750480381556212 1103 98-99 1.0267729291690877 1103 100-101 1.2080671552974387 1104 1 0.3928247242395315 1104 2 0.44875855877702264 1104 3 0.414353644847278 1104 4 0.2464596815134641 1104 5 0.26366919092102137 1104 6 0.2868997550959662 1104 7 0.2423255181615076 1104 8 0.3691485642310255 1104 9 0.44101278380732367 1104 10-11 0.5536182159698129 1104 12-13 0.4957817241453526 1104 14-15 0.5644809879377064 1104 16-17 0.5161801152038095 1104 18-19 0.6193455907021317 1104 20-21 0.6291634860389266 1104 22-23 0.5166450608425777 1104 24-25 0.5609813261591512 1104 26-27 0.7094298499659217 1104 28-29 0.5345106134773658 1104 30-31 0.7601834988842526 1104 32-33 0.7847923654417244 1104 34-35 0.6827350008865025 1104 36-37 0.7155027043947157 1104 38-39 0.5827279318393579 1104 40-41 0.5856384055711032 1104 42-43 0.7375021701443174 1104 44-45 0.7164334986570324 1104 46-47 0.6971293965088066 1104 48-49 0.6945456989066656 1104 50-51 0.7101667119116257 1104 52-53 0.7632468336953835 1104 54-55 0.7395216048621762 1104 56-57 0.7574171121393505 1104 58-59 0.6805739727414775 1104 60-61 0.6746467489521635 1104 62-63 0.5945997725256973 1104 64-65 0.6741601685286511 1104 66-67 0.5196731977689453 1104 68-69 0.5945249895293259 1104 70-71 0.6718836174092502 1104 72-73 0.5948260716030624 1104 74-75 0.7944948591210554 1104 76-77 0.9464834291313764 1104 78-79 1.0138501550659278 1104 80-81 0.7983625457477252 1104 82-83 0.8727864111119921 1104 84-85 1.0375080302460127 1104 86-87 0.9380053684528917 1104 88-89 0.9425490183354057 1104 90-91 0.9036717413774831 1104 92-93 0.8344961847390842 1104 94-95 0.8952055536210146 1104 96-97 0.8309335620093918 1104 98-99 1.0078176102137668 1104 100-101 1.258299205529486 1105 1 0.18255924812039837 1105 2 0.41034781987848135 1105 3 0.351200291937829 1105 4 0.20940568738630105 1105 5 0.29832610948037797 1105 6 0.35426716685362436 1105 7 0.3397210955570884 1105 8 0.35064553792312125 1105 9 0.3860897898599376 1105 10-11 0.3487485569538151 1105 12-13 0.377410536993672 1105 14-15 0.6088616435378711 1105 16-17 0.4191003525142918 1105 18-19 0.38528614322805055 1105 20-21 0.4922011800522341 1105 22-23 0.5606913244010343 1105 24-25 0.422134319507272 1105 26-27 0.4984434533697666 1105 28-29 0.47143816186832765 1105 30-31 0.3918207014970676 1105 32-33 0.4969107809747726 1105 34-35 0.37825426738137935 1105 36-37 0.4205809089851087 1105 38-39 0.4404543056632946 1105 40-41 0.3082446259822049 1105 42-43 0.12772869378547824 1105 44-45 0.3348130755732015 1105 46-47 0.33105340921330395 1105 48-49 0.43767927594268485 1105 50-51 0.39253921745486053 1105 52-53 0.25291244165367033 1105 54-55 0.5420708947285391 1105 56-57 0.4554245430736046 1105 58-59 0.4503684235115415 1105 60-61 0.4085180259941765 1105 62-63 0.23083636388424367 1105 64-65 0.30666750957257705 1105 66-67 0.3632580242806043 1105 68-69 0.5162161263424068 1105 70-71 0.319016115761265 1105 72-73 0.23142581991013245 1105 74-75 0.42572575742512697 1105 76-77 0.3756893978495377 1105 78-79 0.3741695653853405 1105 80-81 0.3720174867197379 1105 82-83 0.3576195147255845 1105 84-85 0.5282385390253062 1105 86-87 0.473878203594019 1105 88-89 0.47612905069592415 1105 90-91 0.3891796217634145 1105 92-93 0.4218965562857946 1105 94-95 0.43237041249318864 1105 96-97 0.3047922544046706 1105 98-99 0.6576845725197522 1105 100-101 0.991040860710168 1106 1 0.09006937148418004 1106 2 0.18526259528105982 1106 3 0.07663865713228901 1106 4 -0.05597099591720678 1106 5 0.13180713405895972 1106 6 0.16163650483272107 1106 7 0.17223074806673822 1106 8 0.14416611424857706 1106 9 0.14539151318604837 1106 10-11 0.1914121787637768 1106 12-13 0.15057726644089087 1106 14-15 0.19798397144069213 1106 16-17 0.13133455535825078 1106 18-19 0.3181258644824041 1106 20-21 0.2820241388995868 1106 22-23 0.3824883391858549 1106 24-25 0.2654521931300238 1106 26-27 0.2900985056345817 1106 28-29 0.39081545728220846 1106 30-31 0.28510891130967053 1106 32-33 0.27067337632272626 1106 34-35 0.11140734205884684 1106 36-37 0.13743583882784804 1106 38-39 0.23390378301520798 1106 40-41 0.2164035863362841 1106 42-43 0.2272441848863309 1106 44-45 0.11781995004349 1106 46-47 -0.07086814648874196 1106 48-49 -0.10055784619687813 1106 50-51 -0.2595419577970475 1106 52-53 -0.21054221509366045 1106 54-55 -0.08070479036422284 1106 56-57 0.05548222270445535 1106 58-59 0.08637864104614579 1106 60-61 -0.16666160485618775 1106 62-63 -0.18738074695482254 1106 64-65 -0.0930958862274025 1106 66-67 -0.09348931539356897 1106 68-69 0.01509361009794219 1106 70-71 -0.05668781116217758 1106 72-73 -0.1292701024931091 1106 74-75 0.16249240211860183 1106 76-77 0.14919490684285464 1106 78-79 0.21818500940078422 1106 80-81 0.14555454293972048 1106 82-83 0.19811108643666486 1106 84-85 0.12545555569353795 1106 86-87 -0.0594060039584825 1106 88-89 0.25043336371975045 1106 90-91 0.17906661677236002 1106 92-93 0.27602830127120015 1106 94-95 0.2885277719432331 1106 96-97 0.22656360763943795 1106 98-99 0.3418558692520257 1106 100-101 0.48168899141927213 1107 1 0.04575867423230662 1107 2 0.12968306617212022 1107 3 0.17114051192826096 1107 4 -0.0656253982186712 1107 5 0.06580100187635907 1107 6 0.1477009306030297 1107 7 0.0702112916355162 1107 8 0.06589183156252432 1107 9 0.13143890511579315 1107 10-11 0.15255893476346927 1107 12-13 0.047062617338006874 1107 14-15 0.13057254741161728 1107 16-17 0.13758313587153737 1107 18-19 0.13195639669528703 1107 20-21 0.26416794236691743 1107 22-23 0.25125206089074936 1107 24-25 0.14527277000942007 1107 26-27 0.2422693739819195 1107 28-29 0.3310304622030955 1107 30-31 0.1773168755764516 1107 32-33 0.4103385667232189 1107 34-35 0.151985735578414 1107 36-37 0.22864164547483057 1107 38-39 0.3134205897378948 1107 40-41 0.06510617548005371 1107 42-43 0.3236074599260803 1107 44-45 0.24252469680705957 1107 46-47 0.3620876033787752 1107 48-49 0.36716397226022934 1107 50-51 0.2586738420364014 1107 52-53 0.2349485487794496 1107 54-55 0.5011598473827661 1107 56-57 0.3978444378607975 1107 58-59 0.34169793092426204 1107 60-61 0.1316624544972811 1107 62-63 0.04840451309514293 1107 64-65 0.20825167982604853 1107 66-67 0.20224654651876506 1107 68-69 0.3462352559454658 1107 70-71 0.17754074512519935 1107 72-73 0.3340465755294524 1107 74-75 0.4438450783242196 1107 76-77 0.526936408849064 1107 78-79 0.5155911340127908 1107 80-81 0.4694397727073003 1107 82-83 0.4288988069302704 1107 84-85 0.526559916356721 1107 86-87 0.4840085240148717 1107 88-89 0.5004475585868846 1107 90-91 0.5096016686309426 1107 92-93 0.5838704922016227 1107 94-95 0.5491578347791766 1107 96-97 0.668827405050294 1107 98-99 0.7482142234044993 1107 100-101 0.8866685508105938 1108 1 0.24446664705618204 1108 2 0.3194620182267798 1108 3 0.30945661823258064 1108 4 0.026211119211595246 1108 5 0.19751518804440593 1108 6 0.2274417509193043 1108 7 0.19247529101612315 1108 8 0.24770667003493685 1108 9 0.4016027554535597 1108 10-11 0.3746397759844555 1108 12-13 0.43136739057998597 1108 14-15 0.4645361674104578 1108 16-17 0.5050291320390059 1108 18-19 0.6258574961281482 1108 20-21 0.608612467462244 1108 22-23 0.6329940716140925 1108 24-25 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-0.7250597118217605 2316 74-75 -0.483713942765327 2316 76-77 -0.784746186103277 2316 78-79 -0.6024047419194467 2316 80-81 -0.5953278471870043 2316 82-83 -0.8145903519574667 2316 84-85 -0.7835415801612804 2316 86-87 -0.6811832327886087 2316 88-89 -0.8856651988586606 2316 90-91 -0.46822776288977863 2316 92-93 -0.8951566476215582 2316 94-95 -0.4871000058885748 2316 96-97 -0.42670341433035475 2316 98-99 -0.6248478351720799 2316 100-101 -0.8673227812385953 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 11.0 4 30.0 5 78.0 6 131.0 7 371.0 8 843.0 9 1561.0 10 2476.0 11 3921.0 12 5270.0 13 6831.0 14 8199.0 15 9990.0 16 11907.0 17 13554.0 18 12294.0 19 10849.0 20 10941.0 21 11642.0 22 13296.0 23 14797.0 24 18399.0 25 23497.0 26 29530.0 27 37568.0 28 47599.0 29 58907.0 30 72107.0 31 86562.0 32 103584.0 33 124715.0 34 152783.0 35 188656.0 36 240851.0 37 298059.0 38 238779.0 39 39976.0 40 10.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.47522568273171 21.294956305381337 14.55589153756853 23.673926474318428 2 15.849199563278567 21.453598347824943 39.30924349850699 23.3879585903895 3 18.61932303703049 25.779904950728444 30.566728124565902 25.034043887675168 4 13.109449279756117 17.615500086319788 38.09534336327156 31.179707270652536 5 13.11036535893445 37.976482498150524 35.47040791521576 13.442744227699258 6 31.095836209654337 38.21978179246713 17.252611839779096 13.43177015809944 7 26.805189584631183 32.71908095230078 22.651711902076002 17.824017560992036 8 25.23945372350277 36.51861330459818 20.2461780007745 17.995754971124544 9 25.582349771858638 15.94100946239456 20.72113927567222 37.755501490074586 10-11 24.56089627565538 26.6586813681747 28.673018074520567 20.10740428164935 12-13 24.970614171703737 23.99909290657148 28.976005168959308 22.05428775276548 14-15 22.141372442189223 25.78405856541874 26.26917478416406 25.805394208227984 16-17 21.874947384371897 28.74673256949472 27.155820130318386 22.222499915814993 18-19 21.79207777010759 27.762636169245535 29.067924671257554 21.37736138938932 20-21 22.42088714158234 26.917287180307444 29.07544870607647 21.58637697203374 22-23 22.254232994164134 26.77491633455235 28.948442195534984 22.02240847574853 24-25 22.090615159176348 27.016545080015682 28.966544159236857 21.926295601571113 26-27 22.134483538802336 27.142849626156913 28.796651460382105 21.926015374658647 28-29 22.01612669849914 27.301316706786103 28.833168909408997 21.849387685305764 30-31 22.2797797955816 27.030344935003704 28.768411758267664 21.92146351114703 32-33 22.238282898774056 27.20690010873192 28.563917633201864 21.990899359292158 34-35 22.443117383660137 27.149677967323644 28.687878004897193 21.71932664411903 36-37 22.40110535958387 26.987074322383215 28.736639450098338 21.875180867934574 38-39 22.287694153302184 27.08183377530833 28.689491518289344 21.94098055310014 40-41 22.358970770228062 26.943433614237726 28.694710101613868 22.002885513920344 42-43 22.27577881874027 27.06624682036942 28.532639295971645 22.125335064918662 44-45 22.252154761312777 27.22962116350665 28.45574145653156 22.062482618649014 46-47 22.406937915110415 27.223402676702456 28.126292492414745 22.24336691577238 48-49 22.42517478248881 27.31437484016203 28.005348454662848 22.25510192268631 50-51 22.409480209059307 27.39361730169257 27.90050282622288 22.296399663025245 52-53 22.515038716106627 27.394020942336716 27.835035536484842 22.255904805071815 54-55 22.31568643703204 27.363727622333002 27.93266997948892 22.38791596114604 56-57 22.38188917851474 27.53300783094855 27.73376090493066 22.351342085606056 58-59 22.34373071689661 27.40266974854241 27.972785184682298 22.280814349878682 60-61 22.312274718870732 27.562749694536294 27.925989605021346 22.198985981571628 62-63 22.351055764738533 27.453543428589633 27.822410014380218 22.372990792291617 64-65 22.117656662905326 27.331085306939872 27.86026246871219 22.690995561442616 66-67 22.1642084860232 27.41965429653809 27.797644834265384 22.618492383173326 68-69 22.181824885180973 27.398374626048117 27.82599650275987 22.59380398601104 70-71 22.183040364686192 27.186969083197198 27.83752484414152 22.792465707975087 72-73 22.262759118168944 27.32704833011273 27.736872311410416 22.673320240307916 74-75 22.241179142101647 27.45979442130288 27.631356157945458 22.667670278650018 76-77 22.17519111061855 27.421213872374782 27.578007699838246 22.825587317168424 78-79 22.064188864756638 27.46521469737412 27.623259748238205 22.84733668963104 80-81 22.106221049582608 27.52558396742426 27.606191979134522 22.76200300385861 82-83 22.21926405468658 27.351708113750778 27.746983019884496 22.682044811678143 84-85 22.225288544104522 27.463069090633574 27.414952098732176 22.896690266529728 86-87 22.044659092822386 27.527365388177056 27.545517779873048 22.882457739127506 88-89 22.073386873369405 27.3845524885541 27.441719503843977 23.10034113423252 90-91 22.071451893273377 27.28549474841292 27.552698066827663 23.090355291486038 92-93 22.053591422046463 27.249643256260548 27.584060087144675 23.11270523454832 94-95 21.84672170394574 27.157398199449627 27.675648371769977 23.32023172483465 96-97 21.872185063896417 27.038197893691173 27.62775600660011 23.4618610358123 98-99 21.714469718653714 27.13685219638678 27.573956526863437 23.57472155809607 100-101 21.763139174650213 27.09825863317226 27.508765763642177 23.62983642853535 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 226.0 1 172.0 2 155.5 3 195.5 4 179.0 5 179.5 6 276.5 7 581.0 8 983.0 9 1172.0 10 1053.5 11 884.5 12 908.5 13 1000.5 14 1160.5 15 1362.5 16 1545.0 17 1710.0 18 1875.0 19 2143.0 20 2492.0 21 3011.5 22 3602.5 23 4440.0 24 5795.0 25 7575.5 26 9861.5 27 12720.5 28 16285.0 29 20703.5 30 25774.0 31 30948.5 32 36074.5 33 39811.0 34 42421.5 35 45505.5 36 49602.0 37 54218.5 38 56917.0 39 58056.5 40 59008.5 41 60303.0 42 61463.0 43 63124.0 44 65639.5 45 68272.0 46 70609.5 47 71836.0 48 72071.5 49 71632.0 50 69968.0 51 67606.5 52 65866.0 53 64753.5 54 63203.0 55 58694.0 56 52699.5 57 46431.5 58 40628.5 59 35336.0 60 29358.5 61 23943.0 62 19367.0 63 14899.5 64 11056.0 65 8129.5 66 5784.5 67 4345.5 68 3165.5 69 2162.5 70 1619.0 71 1211.5 72 855.5 73 667.5 74 496.5 75 329.5 76 225.5 77 144.0 78 83.5 79 46.5 80 28.0 81 18.0 82 11.0 83 7.5 84 4.0 85 2.5 86 1.0 87 0.0 88 0.5 89 0.5 90 0.0 91 0.5 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.017994544811678143 2 0.002683397033320425 3 0.0046301752731803415 4 0.034936777061269846 5 0.0017889313555469499 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 2.6307814052161028E-5 16-17 0.0 18-19 0.0 20-21 0.0 22-23 2.6307814052161028E-5 24-25 5.787719091475427E-4 26-27 0.0024203188927988148 28-29 5.2615628104322056E-5 30-31 0.008418500496691529 32-33 0.025781657771117808 34-35 0.01594253531560958 36-37 9.470813058777971E-4 38-39 8.418500496691529E-4 40-41 0.002525550149007459 42-43 0.010417894364655768 44-45 0.010075892781977673 46-47 0.009365581802569327 48-49 0.01125974441432492 50-51 0.19457259272978297 52-53 0.0515106999141313 54-55 0.02017809337800751 56-57 0.012180517906150556 58-59 0.022861490411327935 60-61 0.05287870624484367 62-63 0.09399781960837135 64-65 0.09857537925344738 66-67 0.10436309834492281 68-69 0.1018638560099675 70-71 0.09144596164531174 72-73 0.07082063542841749 74-75 0.25029254289226 76-77 0.3015401646658697 78-79 0.008865733335578267 80-81 0.0010786203761386022 82-83 0.0 84-85 0.0 86-87 0.0 88-89 2.3677032646944926E-4 90-91 0.00791865202970047 92-93 0.02117779031198963 94-95 0.0024203188927988148 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1900576.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 61.30499482922386 #Duplication Level Percentage of deduplicated Percentage of total 1 78.87380180161497 48.35358011609234 2 12.130182236104424 14.872815185238498 3 3.8487431441143083 7.0784153564681525 4 1.6842538385297225 4.130126914486603 5 0.9541332952503417 2.9246568365856267 6 0.5282604966607733 1.9431004209763163 7 0.3913598665547375 1.6794620216852745 8 0.26854628788736484 1.3170583032273733 9 0.20772784870534897 1.1461279221690495 >10 1.037176051930119 11.135973275712029 >50 0.05370987919020683 2.229559313538316 >100 0.02090152702455819 2.325676643209871 >500 9.457850546196985E-4 0.37919801515493623 >1k 2.5794137853264406E-4 0.48424967545571157 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3960 0.20835788729311536 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3092 0.1626875220985638 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2135 0.11233436600272759 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 5.2615628104322056E-5 2 0.0 0.0 0.0 0.0 5.2615628104322056E-5 3 0.0 5.2615628104322056E-5 0.0 0.0 5.2615628104322056E-5 4 0.0 5.2615628104322056E-5 0.0 0.0 5.2615628104322056E-5 5 0.0 5.2615628104322056E-5 0.0 0.0 5.2615628104322056E-5 6 0.0 5.2615628104322056E-5 0.0 0.0 5.2615628104322056E-5 7 0.0 5.2615628104322056E-5 0.0 0.0 5.2615628104322056E-5 8 0.0 5.2615628104322056E-5 0.0 0.0 1.5784688431296618E-4 9 0.0 5.2615628104322056E-5 0.0 0.0 1.5784688431296618E-4 10-11 0.0 5.2615628104322056E-5 0.0 2.6307814052161028E-5 1.5784688431296618E-4 12-13 0.0 5.2615628104322056E-5 0.0 5.2615628104322056E-5 3.1569376862593235E-4 14-15 0.0 5.2615628104322056E-5 0.0 5.2615628104322056E-5 3.9461721078241544E-4 16-17 0.0 5.2615628104322056E-5 0.0 2.1046251241728823E-4 4.7354065293889853E-4 18-19 0.0 5.2615628104322056E-5 0.0 2.8938595457377134E-4 4.7354065293889853E-4 20-21 0.0 5.2615628104322056E-5 0.0 3.4200158267809336E-4 4.998484669910595E-4 22-23 0.0 5.2615628104322056E-5 0.0 3.9461721078241544E-4 6.050797231997036E-4 24-25 0.0 5.2615628104322056E-5 0.0 5.261562810432206E-4 8.681578637213139E-4 26-27 0.0 5.2615628104322056E-5 0.0 6.576953513040258E-4 0.0011312360042429242 28-29 0.0 5.2615628104322056E-5 0.0 0.0022098563803815267 0.0011575438182950854 30-31 0.0 5.2615628104322056E-5 0.0 0.00791865202970047 0.0011575438182950854 32-33 0.0 5.2615628104322056E-5 0.0 0.02501873116360514 0.0011575438182950854 34-35 0.0 5.2615628104322056E-5 0.0 0.050984543633088075 0.0012101594463994074 36-37 0.0 5.2615628104322056E-5 0.0 0.08892041149630428 0.0012101594463994074 38-39 0.0 5.2615628104322056E-5 0.0 0.14240419746434765 0.0012890828885558903 40-41 0.0 5.2615628104322056E-5 0.0 0.19722968194905122 0.0013153907026080514 42-43 0.0 5.2615628104322056E-5 0.0 0.25305486336773697 0.0013416985166602126 44-45 0.0 5.2615628104322056E-5 0.0 0.32290210967622446 0.0013943141447645344 46-47 0.0 5.2615628104322056E-5 0.0 0.4054560301719058 0.0015258532150253397 48-49 0.0 5.2615628104322056E-5 0.0 0.49003565234960345 0.0018678547977034332 50-51 0.0 5.2615628104322056E-5 0.0 0.5772460559325172 0.0018941626117555941 52-53 0.0 5.2615628104322056E-5 0.0 0.6652983095651003 0.0019730860539120773 54-55 0.0 1.0523125620864411E-4 0.0 0.7664518545956595 0.0021046251241728826 56-57 0.0 1.0523125620864411E-4 0.0 0.8872310289091307 0.0022098563803815263 58-59 0.0 1.0523125620864411E-4 0.0 1.0130086878925124 0.0022887798225380094 60-61 0.0 1.0523125620864411E-4 0.0 1.1483886990049332 0.0024466267068509757 62-63 0.0 1.0523125620864411E-4 0.0 1.2843211742124492 0.002788628289529069 64-65 0.0 1.0523125620864411E-4 0.0 1.434749254962706 0.00281493610358123 66-67 0.0 1.0523125620864411E-4 0.0 1.6016986429377198 0.002841243917633391 68-69 0.0 1.0523125620864411E-4 0.0 1.7926407573283045 0.002841243917633391 70-71 0.0 1.0523125620864411E-4 0.0 1.9997884851750207 0.002893859545737713 72-73 0.0 1.0523125620864411E-4 0.0 2.2186958059030526 0.0031569376862593235 74-75 0.0 1.0523125620864411E-4 0.0 2.4434697691647163 0.0031569376862593235 76-77 0.0 1.0523125620864411E-4 0.0 2.697129712255653 0.0031832455003114844 78-79 0.0 1.0523125620864411E-4 0.0 2.9849371979862944 0.0032358611284158067 80-81 0.0 1.0523125620864411E-4 0.0 3.2892659909416935 0.0033937080127287725 82-83 0.0 1.0523125620864411E-4 0.0 3.6150356523496034 0.0035778627110939 84-85 0.0 1.0523125620864411E-4 0.0 3.9551956880440455 0.0035778627110939 86-87 0.0 1.0523125620864411E-4 0.0 4.313008266967488 0.003630478339198222 88-89 0.0 1.0523125620864411E-4 0.0 4.706757319886182 0.003735709595406866 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2240 0.0 56.610348 1 GTATCAA 4255 0.0 41.52178 1 ATCAACG 5185 0.0 33.790596 3 CAACGCA 5285 0.0 33.309864 5 TCAACGC 5375 0.0 32.683613 4 TATCAAC 5555 0.0 31.627054 2 AACGCAG 5605 0.0 31.576622 6 ACGCAGA 6460 0.0 27.397366 7 CGCAGAG 6660 0.0 26.503376 8 GTACATG 4725 0.0 24.324623 1 GCAGAGT 7295 0.0 23.676014 9 TACATGG 4930 0.0 22.537722 2 ACATGGG 4975 0.0 21.855495 3 AGAGTAC 7010 0.0 20.272688 10-11 CAGAGTA 7190 0.0 19.468193 10-11 CATGGGG 3555 0.0 19.098675 4 GTGGTAT 1100 0.0 18.99733 1 GTCCTAC 555 0.0 18.826183 1 GAGTACT 5255 0.0 18.420013 12-13 TATACTG 1030 0.0 17.966822 5 >>END_MODULE