FastQCFastQC Report
Sat 18 Jun 2016
SRR1930911_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1930911_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2038739
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN37870.18575207517980477No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25180.1235077172703323No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA22550.061.5068661
GTATCAA44150.046.4398231
TATCAAC55500.036.6579172
ATCAACG54300.036.4397283
TCAACGC54300.035.7865074
CAACGCA55600.034.6422585
AACGCAG60300.031.1723066
GTGGTAT9550.030.5200821
GTACATG52900.029.2587761
TACATGG54250.027.5944822
ACGCAGA69100.026.8225367
CGCAGAG70650.025.8490688
ACATGGG57800.024.8494573
CATGGGG36950.024.460554
TGGTATC10900.023.9652842
GAGTACA39550.022.8711011
GCAGAGT74050.022.8556529
GAGTACT49000.021.29575212-13
AGTACAT42000.020.8116112
CAGAGTA72050.020.79230310-11
Produced by FastQC (version 0.11.5)