##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1930911_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2038739 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 22.67405440323651 28.0 16.0 31.0 2.0 31.0 2 22.298462922424108 30.0 10.0 31.0 2.0 33.0 3 22.92192723050866 30.0 16.0 31.0 2.0 33.0 4 24.639381009535796 33.0 10.0 35.0 2.0 37.0 5 24.515751648445438 32.0 10.0 35.0 2.0 37.0 6 24.863737339600608 32.0 17.0 35.0 2.0 37.0 7 24.36452336468768 32.0 11.0 35.0 2.0 37.0 8 24.11474690973195 30.0 11.0 35.0 2.0 37.0 9 22.503314058346852 27.0 10.0 34.0 2.0 37.0 10-11 22.499335618732953 27.0 10.0 33.5 2.0 37.0 12-13 22.2200517084335 27.0 10.0 33.0 2.0 37.0 14-15 24.264204981608728 29.0 13.5 35.0 2.0 37.5 16-17 26.26046786763779 32.5 17.0 36.5 2.0 38.5 18-19 27.280364234951115 33.5 17.5 38.0 2.0 39.5 20-21 27.480970099654737 34.0 18.0 38.5 2.0 40.0 22-23 27.87984165702427 34.5 18.0 39.0 2.0 40.0 24-25 27.835631485933217 35.0 18.0 39.0 2.0 40.0 26-27 27.660470271084236 34.5 17.0 39.0 2.0 40.0 28-29 27.76688506964354 35.0 17.5 39.0 2.0 40.0 30-31 27.839349960931735 35.0 17.5 39.0 2.0 40.0 32-33 27.686729885483132 35.0 18.0 38.5 2.0 40.0 34-35 24.342413128899775 31.0 6.0 38.0 2.0 40.0 36-37 22.623022368238406 25.0 5.5 37.5 2.0 40.0 38-39 21.59330522445492 25.5 2.0 37.0 2.0 39.5 40-41 23.377486524758687 30.0 5.0 37.0 2.0 39.0 42-43 23.36201544189815 30.0 5.0 37.0 2.0 40.0 44-45 21.053847255582987 22.5 2.0 36.5 2.0 39.5 46-47 22.283534331760954 26.0 4.5 36.0 2.0 39.0 48-49 21.681156342229194 28.0 2.0 37.0 2.0 39.0 50-51 19.143860739408034 14.5 2.0 34.5 2.0 38.5 52-53 22.755058151141466 27.5 2.0 36.0 2.0 39.0 54-55 24.872679141371208 32.5 2.0 37.5 2.0 39.5 56-57 25.19775655441918 33.0 2.0 38.0 2.0 40.0 58-59 24.823444786213436 32.5 2.0 38.0 2.0 40.0 60-61 24.57602665176857 32.0 2.0 37.5 2.0 40.0 62-63 24.14858473791888 31.5 2.0 37.0 2.0 39.5 64-65 23.673835150060896 31.0 2.0 36.0 2.0 39.0 66-67 23.292960501564938 31.0 2.0 36.0 2.0 39.0 68-69 22.867350357255148 30.0 2.0 35.5 2.0 39.0 70-71 22.279307944763897 29.5 2.0 35.0 2.0 38.0 72-73 21.86767850126966 29.0 2.0 35.0 2.0 37.5 74-75 21.356883347991086 29.0 2.0 34.0 2.0 37.0 76-77 20.8250901660291 27.5 2.0 34.0 2.0 36.0 78-79 20.36476125683572 27.0 2.0 34.0 2.0 36.0 80-81 19.900048755627864 26.0 2.0 34.0 2.0 35.0 82-83 19.475046094669302 26.0 2.0 34.0 2.0 35.0 84-85 18.992826202863633 25.5 2.0 33.0 2.0 35.0 86-87 18.453974000595466 24.5 2.0 33.0 2.0 35.0 88-89 17.924833683958564 23.5 2.0 32.5 2.0 35.0 90-91 17.36640761764993 20.0 2.0 32.0 2.0 34.5 92-93 16.731378562925414 18.0 2.0 32.0 2.0 34.0 94-95 16.073907204404293 9.0 2.0 32.0 2.0 34.0 96-97 15.293580002148387 2.0 2.0 31.0 2.0 34.0 98-99 14.381280781895082 2.0 2.0 31.0 2.0 34.0 100-101 12.952984908808826 2.0 2.0 29.0 2.0 33.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 8.08181081616242 1101 2 8.635084654298325 1101 3 8.128274295086602 1101 4 10.582413955075108 1101 5 10.345512669386622 1101 6 9.89504050542918 1101 7 10.10627306794185 1101 8 9.922605061804209 1101 9 11.368358563637308 1101 10-11 10.801794117515609 1101 12-13 10.230175678378064 1101 14-15 8.954235697205416 1101 16-17 8.138328662345035 1101 18-19 8.214384308456289 1101 20-21 8.633103619009578 1101 22-23 8.441360068212617 1101 24-25 8.650942022776757 1101 26-27 8.684952661353776 1101 28-29 8.53171127306581 1101 30-31 8.43500745338395 1101 32-33 8.25927144516179 1101 34-35 11.213383953749908 1101 36-37 4.566514779775446 1101 38-39 8.242943705364826 1101 40-41 10.011394525937977 1101 42-43 10.665145937918059 1101 44-45 4.022544902370438 1101 46-47 5.630794191243634 1101 48-49 9.649687664995092 1101 50-51 -5.915781626838182 1101 52-53 5.28471084321032 1101 54-55 8.066764105656635 1101 56-57 8.641971437485385 1101 58-59 8.980251630845029 1101 60-61 8.818248504095745 1101 62-63 8.860175484030332 1101 64-65 8.740576091159092 1101 66-67 8.633075699648899 1101 68-69 8.829649953485426 1101 70-71 8.955719443994987 1101 72-73 8.57535591642863 1101 74-75 8.733749040099696 1101 76-77 8.774044044852072 1101 78-79 8.737490579990542 1101 80-81 8.647720416299347 1101 82-83 8.406393337518985 1101 84-85 8.56691804179843 1101 86-87 8.501131963241487 1101 88-89 8.48928675493233 1101 90-91 8.550004651712868 1101 92-93 8.738043335691149 1101 94-95 8.545590652067395 1101 96-97 8.335673329053728 1101 98-99 8.496532767434777 1101 100-101 8.358252131241123 1102 1 7.846340721399805 1102 2 8.370449723032753 1102 3 8.062098982074247 1102 4 10.390440292036875 1102 5 10.222722021269483 1102 6 9.629839564326382 1102 7 9.822973728540347 1102 8 9.52966311532182 1102 9 10.197261378682775 1102 10-11 9.716646711419312 1102 12-13 8.712987288211803 1102 14-15 8.094427179442317 1102 16-17 7.732409804193235 1102 18-19 7.680229865833553 1102 20-21 8.31196718519772 1102 22-23 8.0703485566641 1102 24-25 8.339868300158546 1102 26-27 8.575239275862593 1102 28-29 8.437410402850148 1102 30-31 8.222124369315292 1102 32-33 8.27026580028458 1102 34-35 10.939975870007412 1102 36-37 0.2860576664908514 1102 38-39 2.8091469600844725 1102 40-41 8.823054045905973 1102 42-43 10.233144637595803 1102 44-45 0.5591152698093218 1102 46-47 0.7351694059707619 1102 48-49 8.329013193495637 1102 50-51 -6.925222988393003 1102 52-53 4.3477442906298975 1102 54-55 7.5502265289528445 1102 56-57 8.183356802554119 1102 58-59 8.549990000788885 1102 60-61 8.343125538839136 1102 62-63 8.47128062747893 1102 64-65 8.431481880854804 1102 66-67 8.506496286489984 1102 68-69 8.275230706106726 1102 70-71 8.276935788915253 1102 72-73 8.388900033883825 1102 74-75 8.259286782061835 1102 76-77 8.293650860454196 1102 78-79 8.343183403864288 1102 80-81 8.233074177883736 1102 82-83 8.059816359746232 1102 84-85 8.321148329304467 1102 86-87 8.33421461850823 1102 88-89 8.244135347844487 1102 90-91 8.363026900705009 1102 92-93 8.281219688091529 1102 94-95 8.178772788509901 1102 96-97 8.126139553291909 1102 98-99 8.24161328738972 1102 100-101 7.906321109205226 1103 1 7.584534829049023 1103 2 8.050741631645824 1103 3 7.734572580969704 1103 4 9.832882170283764 1103 5 9.534140799369126 1103 6 8.533485254294185 1103 7 8.864384030323436 1103 8 8.548392934916091 1103 9 8.932719908085545 1103 10-11 7.99699673086241 1103 12-13 7.0787637231779215 1103 14-15 6.9620325763218105 1103 16-17 7.1358527616412495 1103 18-19 7.410898511763833 1103 20-21 8.147969356707687 1103 22-23 8.302398645578037 1103 24-25 8.268131900495849 1103 26-27 8.38268958673369 1103 28-29 8.249516133976144 1103 30-31 8.02784948761234 1103 32-33 7.902966796033315 1103 34-35 10.890573035247524 1103 36-37 -4.104114393996841 1103 38-39 -2.3446751377601807 1103 40-41 7.468800049147944 1103 42-43 9.755877441377816 1103 44-45 0.26326516953537293 1103 46-47 -0.6921433395256535 1103 48-49 8.323448316904685 1103 50-51 -7.241585228587347 1103 52-53 4.236994660922594 1103 54-55 7.429061952965284 1103 56-57 8.05094483172314 1103 58-59 8.297424822720696 1103 60-61 8.188066619700582 1103 62-63 8.026927864156036 1103 64-65 8.134160003656532 1103 66-67 8.133293899139794 1103 68-69 8.204899452529414 1103 70-71 8.249342101586471 1103 72-73 8.14425333025343 1103 74-75 8.057576772580596 1103 76-77 8.141276181046841 1103 78-79 7.956405256157915 1103 80-81 7.759770205811886 1103 82-83 7.547204343475201 1103 84-85 7.46645719055066 1103 86-87 7.402148472654414 1103 88-89 7.574750361410963 1103 90-91 7.737146936707511 1103 92-93 7.783104576217259 1103 94-95 7.497987811688697 1103 96-97 7.318270451213467 1103 98-99 7.2947626322228825 1103 100-101 6.798550096460168 1104 1 7.189007785457878 1104 2 7.744963805590817 1104 3 7.139476411661455 1104 4 9.318424453150708 1104 5 8.710711930366195 1104 6 8.054259976052272 1104 7 7.983012474202752 1104 8 7.316237317523086 1104 9 7.160806393904288 1104 10-11 6.226542821950897 1104 12-13 5.6677623717704435 1104 14-15 5.9149570583089 1104 16-17 6.3139374903107885 1104 18-19 7.082957496600166 1104 20-21 7.736351938140896 1104 22-23 7.848237982504472 1104 24-25 8.007261802809008 1104 26-27 8.280482181361492 1104 28-29 8.186531706577824 1104 30-31 7.816643028970834 1104 32-33 7.786617214927038 1104 34-35 10.629723703869413 1104 36-37 -4.106519984500142 1104 38-39 -2.543517868646923 1104 40-41 6.842968145495096 1104 42-43 9.244579397990076 1104 44-45 0.06334535943527797 1104 46-47 -0.9881982733343726 1104 48-49 7.772830918571742 1104 50-51 -7.3147739006191745 1104 52-53 3.7298127129150416 1104 54-55 6.752461855629868 1104 56-57 7.420535075178815 1104 58-59 7.453192904584679 1104 60-61 7.4446774942177605 1104 62-63 7.563937047124252 1104 64-65 7.607760807046443 1104 66-67 7.302575949274988 1104 68-69 7.361845889297069 1104 70-71 7.362703144630238 1104 72-73 7.196627345318053 1104 74-75 7.127019841478948 1104 76-77 6.911472800717757 1104 78-79 6.818816746422776 1104 80-81 6.856466339338187 1104 82-83 6.769493195375212 1104 84-85 6.60162168633936 1104 86-87 6.510260964281333 1104 88-89 6.508051853070896 1104 90-91 6.443960096370603 1104 92-93 6.6166421648334826 1104 94-95 6.678749370801858 1104 96-97 6.864527031204753 1104 98-99 6.582727331627899 1104 100-101 6.598449033757628 1105 1 6.511173185031744 1105 2 7.323005135036308 1105 3 6.978144553308663 1105 4 8.930063440076271 1105 5 8.523000549432709 1105 6 7.520757197172575 1105 7 7.641311284539611 1105 8 6.629505394554695 1105 9 6.09186195922943 1105 10-11 5.300199287804823 1105 12-13 4.59502054380334 1105 14-15 5.0082067730596584 1105 16-17 5.937358293261841 1105 18-19 6.786504299968357 1105 20-21 7.437702935797564 1105 22-23 7.506725425403502 1105 24-25 7.79008642462372 1105 26-27 7.892121270526928 1105 28-29 7.5203161563023855 1105 30-31 7.2826044109267265 1105 32-33 7.07611237489154 1105 34-35 9.940736369344645 1105 36-37 -4.564091664873768 1105 38-39 -2.9636353013628636 1105 40-41 6.090755265725097 1105 42-43 8.485819179114959 1105 44-45 -4.100913421947251 1105 46-47 -5.331961334477906 1105 48-49 5.8936396972960345 1105 50-51 -11.880789776426358 1105 52-53 0.43734227046177665 1105 54-55 5.480680060052354 1105 56-57 6.541601907337757 1105 58-59 6.941528868890696 1105 60-61 6.7957963051271655 1105 62-63 6.832999364370483 1105 64-65 6.438435017652399 1105 66-67 6.177560817774065 1105 68-69 6.283800061297249 1105 70-71 6.454455965428281 1105 72-73 6.289410948496489 1105 74-75 6.0493579241950926 1105 76-77 5.940325301300522 1105 78-79 6.033499516550741 1105 80-81 5.773448177447488 1105 82-83 5.835841964202682 1105 84-85 5.6693181811476165 1105 86-87 5.865038122258291 1105 88-89 5.815578241181626 1105 90-91 5.6164548105692695 1105 92-93 5.504829983566285 1105 94-95 5.450773577113738 1105 96-97 5.378525918834955 1105 98-99 5.101170075255288 1105 100-101 4.8697202434188895 1106 1 6.250869380280431 1106 2 7.219702229173485 1106 3 6.6669447531341 1106 4 8.265092953445912 1106 5 7.364864426910049 1106 6 6.946869867099579 1106 7 6.67439086999147 1106 8 5.7970374689008395 1106 9 4.88669167975905 1106 10-11 4.158032290133132 1106 12-13 3.6635387876539376 1106 14-15 4.379992105774722 1106 16-17 5.635831221886182 1106 18-19 6.396882285431303 1106 20-21 7.203186147445461 1106 22-23 6.95556243239006 1106 24-25 7.306042612162994 1106 26-27 7.4703452486015465 1106 28-29 7.392275582448065 1106 30-31 6.911366418178137 1106 32-33 6.806630718980838 1106 34-35 10.025939466801926 1106 36-37 -0.5966403112031173 1106 38-39 0.5325261880701042 1106 40-41 7.187196506696484 1106 42-43 8.49101824665058 1106 44-45 -4.189467588739557 1106 46-47 -5.353852997633499 1106 48-49 5.718212501151722 1106 50-51 -11.736150631666195 1106 52-53 0.42004926133679277 1106 54-55 5.3871646888961635 1106 56-57 6.278427139944508 1106 58-59 6.714958544109162 1106 60-61 6.635124978855089 1106 62-63 6.695132342005845 1106 64-65 6.430212795792375 1106 66-67 6.3955721431171 1106 68-69 6.312965040340842 1106 70-71 6.379724042825593 1106 72-73 6.27308334805609 1106 74-75 6.105558115545062 1106 76-77 6.116417393771126 1106 78-79 5.758327684602953 1106 80-81 5.741482423224628 1106 82-83 5.717127969657952 1106 84-85 5.493238109171344 1106 86-87 5.440178130456676 1106 88-89 5.595162409497885 1106 90-91 5.405335710696484 1106 92-93 5.467328689835114 1106 94-95 5.20920452772703 1106 96-97 4.831775771532962 1106 98-99 4.85203259665788 1106 100-101 4.525657634164819 1107 1 6.060777569337247 1107 2 7.018003508393097 1107 3 6.556954613594872 1107 4 7.678403661540731 1107 5 7.684980274630412 1107 6 6.139314987186992 1107 7 6.128664836445594 1107 8 5.157854170957524 1107 9 3.6894983060699253 1107 10-11 3.4522056819183824 1107 12-13 3.3643964751067834 1107 14-15 4.313328891792565 1107 16-17 5.881570355323301 1107 18-19 6.6907344760073 1107 20-21 7.474463264981946 1107 22-23 7.415879510687809 1107 24-25 7.408188252488724 1107 26-27 7.499874434448387 1107 28-29 7.414168750296255 1107 30-31 7.222181343117793 1107 32-33 6.96713119575595 1107 34-35 9.864988414697873 1107 36-37 -0.6200212814277748 1107 38-39 0.7766724153521984 1107 40-41 7.681749084480895 1107 42-43 8.995820524609965 1107 44-45 -3.7678558146791694 1107 46-47 -4.919378044320904 1107 48-49 6.276803237192976 1107 50-51 -11.723930362580212 1107 52-53 0.7946697960763842 1107 54-55 5.696198637924386 1107 56-57 6.8586474212164354 1107 58-59 7.181089333829508 1107 60-61 7.290887356982942 1107 62-63 7.04944763912458 1107 64-65 7.069420160977742 1107 66-67 6.963807750246282 1107 68-69 6.872395083367383 1107 70-71 7.111018667503558 1107 72-73 6.9613534617204955 1107 74-75 7.107153661988239 1107 76-77 6.824879821962906 1107 78-79 6.845981392532249 1107 80-81 6.580382296389246 1107 82-83 6.218798358115425 1107 84-85 6.407954973808849 1107 86-87 6.425937736281963 1107 88-89 6.261977033850883 1107 90-91 6.1862654170862825 1107 92-93 5.93512631377887 1107 94-95 5.657927694529265 1107 96-97 5.6887976686612465 1107 98-99 5.3222787832186125 1107 100-101 4.931873075585457 1108 1 5.791362539866675 1108 2 5.9324877681678 1108 3 6.469589118036829 1108 4 7.554793010853015 1108 5 6.79879443593358 1108 6 6.4101802982281875 1108 7 6.061808857578509 1108 8 4.661825974281328 1108 9 1.9466207606235315 1108 10-11 2.6851871822801208 1108 12-13 2.8304734175896478 1108 14-15 4.196315500247817 1108 16-17 5.372071073583797 1108 18-19 6.365737134300865 1108 20-21 7.77969697589722 1108 22-23 7.891583020260796 1108 24-25 7.4718184181785965 1108 26-27 7.764047621752795 1108 28-29 7.801779017355727 1108 30-31 7.525863340319805 1108 32-33 7.275119211543082 1108 34-35 10.251036439116643 1108 36-37 -4.427610448933628 1108 38-39 -2.698703550755077 1108 40-41 6.225428600608918 1108 42-43 8.65380968711197 1108 44-45 -3.937650719512259 1108 46-47 -5.015002180375497 1108 48-49 6.132563811467779 1108 50-51 -11.837528211760102 1108 52-53 0.8954426818990271 1108 54-55 5.8976292168486 1108 56-57 6.8275204715405415 1108 58-59 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-16.688604460480377 2313 84-85 -16.21028765905995 2313 86-87 -15.700118201936892 2313 88-89 -15.169094209083827 2313 90-91 -14.606227916726514 2313 92-93 -13.988256938317967 2313 94-95 -13.622161790929349 2313 96-97 -12.768543745823587 2313 98-99 -11.77898046417512 2313 100-101 -10.373236160529968 2314 1 -20.15085554028244 2314 2 -19.773942800331735 2314 3 -20.35892905530893 2314 4 -21.992737278056584 2314 5 -21.902044333870705 2314 6 -22.26829127679511 2314 7 -21.753661785478343 2314 8 -21.53342099682771 2314 9 -19.94853301384059 2314 10-11 -19.952044877365733 2314 12-13 -19.68280710431156 2314 14-15 -21.713516745791328 2314 16-17 -23.73207617711518 2314 18-19 -24.720003779026257 2314 20-21 -24.896677674615365 2314 22-23 -25.28479163025179 2314 24-25 -25.21597282133678 2314 26-27 -25.060975677408436 2314 28-29 -25.156748475654524 2314 30-31 -25.223903668067887 2314 32-33 -25.077164106257474 2314 34-35 -21.88736057859072 2314 36-37 -20.13590495033158 2314 38-39 -19.1653857501959 2314 40-41 -20.92764688867347 2314 42-43 -20.89232265212382 2314 44-45 -18.61752024420937 2314 46-47 -19.74286797720681 2314 48-49 -19.267715778466982 2314 50-51 -16.726391212692068 2314 52-53 -20.258797765444868 2314 54-55 -22.278745433663982 2314 56-57 -22.587437589969245 2314 58-59 -22.21309411136065 2314 60-61 -21.985846423777684 2314 62-63 -21.558158624857523 2314 64-65 -21.097022792042395 2314 66-67 -20.71359717145394 2314 68-69 -20.286217892024116 2314 70-71 -19.700167014823066 2314 72-73 -19.311800900695612 2314 74-75 -18.783002937564333 2314 76-77 -18.276909887209353 2314 78-79 -17.83835865221048 2314 80-81 -17.36972956043402 2314 82-83 -16.96308083651545 2314 84-85 -16.49450587630301 2314 86-87 -15.976299400183361 2314 88-89 -15.4413786708471 2314 90-91 -14.879730108640786 2314 92-93 -14.250556012843052 2314 94-95 -13.622161790929349 2314 96-97 -12.841851100913699 2314 98-99 -11.944835310741121 2314 100-101 -10.57148262911253 2315 1 -20.15085554028244 2315 2 -19.773942800331735 2315 3 -20.35892905530893 2315 4 -21.663835259346587 2315 5 -21.902044333870705 2315 6 -22.26829127679511 2315 7 -21.753661785478343 2315 8 -21.53342099682771 2315 9 -19.64080283166432 2315 10-11 -19.55322656126529 2315 12-13 -19.05602226925494 2315 14-15 -21.052758498622445 2315 16-17 -23.40489744742537 2315 18-19 -24.720003779026257 2315 20-21 -24.896677674615365 2315 22-23 -25.28479163025179 2315 24-25 -25.21597282133678 2315 26-27 -25.060975677408436 2315 28-29 -25.156748475654524 2315 30-31 -25.223903668067887 2315 32-33 -25.077164106257474 2315 34-35 -21.88736057859072 2315 36-37 -20.13590495033158 2315 38-39 -19.1653857501959 2315 40-41 -20.92764688867347 2315 42-43 -20.89232265212382 2315 44-45 -18.61752024420937 2315 46-47 -19.74286797720681 2315 48-49 -19.267715778466982 2315 50-51 -16.726391212692068 2315 52-53 -20.258797765444868 2315 54-55 -22.278745433663982 2315 56-57 -22.587437589969245 2315 58-59 -22.21309411136065 2315 60-61 -21.985846423777684 2315 62-63 -21.558158624857523 2315 64-65 -21.097022792042395 2315 66-67 -20.71359717145394 2315 68-69 -20.286217892024116 2315 70-71 -19.700167014823066 2315 72-73 -19.311800900695612 2315 74-75 -18.783002937564333 2315 76-77 -18.276909887209353 2315 78-79 -17.83835865221048 2315 80-81 -17.36972956043402 2315 82-83 -16.96308083651545 2315 84-85 -16.49450587630301 2315 86-87 -15.976299400183361 2315 88-89 -15.4413786708471 2315 90-91 -14.879730108640786 2315 92-93 -14.250556012843052 2315 94-95 -13.622161790929349 2315 96-97 -12.841851100913699 2315 98-99 -11.944835310741121 2315 100-101 -10.57148262911253 2316 1 -20.15085554028244 2316 2 -19.773942800331735 2316 3 -20.35892905530893 2316 4 -21.992737278056584 2316 5 -21.902044333870705 2316 6 -22.26829127679511 2316 7 -21.753661785478343 2316 8 -21.53342099682771 2316 9 -19.94853301384059 2316 10-11 -19.952044877365733 2316 12-13 -19.68280710431156 2316 14-15 -21.713516745791328 2316 16-17 -23.73207617711518 2316 18-19 -24.720003779026257 2316 20-21 -24.896677674615365 2316 22-23 -25.28479163025179 2316 24-25 -25.21597282133678 2316 26-27 -25.060975677408436 2316 28-29 -25.156748475654524 2316 30-31 -25.223903668067887 2316 32-33 -25.077164106257474 2316 34-35 -21.88736057859072 2316 36-37 -20.13590495033158 2316 38-39 -19.1653857501959 2316 40-41 -20.92764688867347 2316 42-43 -20.89232265212382 2316 44-45 -18.61752024420937 2316 46-47 -19.74286797720681 2316 48-49 -19.267715778466982 2316 50-51 -16.726391212692068 2316 52-53 -20.258797765444868 2316 54-55 -22.278745433663982 2316 56-57 -22.587437589969245 2316 58-59 -22.21309411136065 2316 60-61 -21.985846423777684 2316 62-63 -21.558158624857523 2316 64-65 -21.097022792042395 2316 66-67 -20.71359717145394 2316 68-69 -20.286217892024116 2316 70-71 -19.700167014823066 2316 72-73 -19.311800900695612 2316 74-75 -18.783002937564333 2316 76-77 -18.276909887209353 2316 78-79 -17.83835865221048 2316 80-81 -17.36972956043402 2316 82-83 -16.96308083651545 2316 84-85 -16.49450587630301 2316 86-87 -15.976299400183361 2316 88-89 -15.4413786708471 2316 90-91 -14.879730108640786 2316 92-93 -14.250556012843052 2316 94-95 -13.622161790929349 2316 96-97 -12.841851100913699 2316 98-99 -11.944835310741121 2316 100-101 -10.57148262911253 >>END_MODULE >>Per sequence quality scores fail #Quality Count 2 330423.0 3 7980.0 4 8655.0 5 6234.0 6 8384.0 7 13617.0 8 20369.0 9 24414.0 10 25265.0 11 24440.0 12 24822.0 13 24509.0 14 25822.0 15 28957.0 16 31245.0 17 32584.0 18 33748.0 19 35796.0 20 38311.0 21 41811.0 22 45757.0 23 49888.0 24 55124.0 25 60976.0 26 67124.0 27 74370.0 28 83039.0 29 91591.0 30 98947.0 31 101968.0 32 102815.0 33 105247.0 34 103885.0 35 95081.0 36 74562.0 37 35769.0 38 5167.0 39 43.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.17243711787631 19.768627292314758 14.49319051236273 23.565745077446206 2 14.955343485266223 20.483839168562508 40.15470789243871 24.406109453732565 3 17.906928545721097 26.463499623362445 30.36285784236882 25.266713988547636 4 12.018932558705767 16.84696035666918 40.062691254958 31.07141582966706 5 12.473226353196761 37.96910456938986 36.168754350424976 13.3889147269884 6 29.392232946620656 38.67490374729924 18.111752924712867 13.821110381367241 7 25.30486182287026 35.30983483754151 22.416221298580602 16.969082041007628 8 24.209144197471744 38.05363785909698 20.306798178520914 17.43041976491037 9 24.903386766057594 20.59104269567589 20.03171621061988 34.473854327646634 10-11 23.07177093033409 30.97743822320845 27.173082724772048 18.777708121685414 12-13 23.700508857729268 28.509487296326807 27.517665321644035 20.272338524299894 14-15 21.83183440776494 27.727857581176224 26.08526390107751 24.35504410998133 16-17 21.35262421537708 29.516421334416087 27.260624414812547 21.870330035394286 18-19 21.343174853619832 28.042404647684364 29.691917625813986 20.92250287288182 20-21 22.31904989886449 26.945598715361374 29.69879255880864 21.0365588269655 22-23 22.107413400693478 26.85927923826059 29.42371167293041 21.609595688115522 24-25 21.805969410597527 26.994790893385957 29.591201374670117 21.608038321346402 26-27 21.685123061770465 27.187007907643252 29.390741529535415 21.73712750105087 28-29 21.66903047606356 27.304602177317623 29.46501011501384 21.56135723160498 30-31 21.916966605520756 27.11273991106632 29.33150592373352 21.638787559679404 32-33 21.79679148544645 27.160669083183137 29.16590733822415 21.876632093146263 34-35 22.19418963238681 27.197309032040195 29.05762198419123 21.55087935138177 36-37 22.63826235770846 27.13088233696503 28.634876692912282 21.595978612414232 38-39 22.412631085364225 27.212767080159516 28.733418919896724 21.64118291457953 40-41 22.362693838749927 27.073109143841723 28.932713445428078 21.631483571980276 42-43 22.40544221240191 27.250917674459046 28.398363157815076 21.945276955323965 44-45 22.696653938913126 27.382426821758816 28.213282143285205 21.707637096042852 46-47 22.17011409529952 27.376673632720067 28.41809163640831 22.035120635572103 48-49 22.66637972443002 27.552005437844805 27.727147702172566 22.054467135552606 50-51 23.055614179943547 27.5613920762043 27.48153948877065 21.9014542550815 52-53 22.032773981494238 27.62394123210366 28.126380837529254 22.21690394887285 54-55 21.813097876767475 27.610347916018306 28.216403104084264 22.36015110312995 56-57 21.93683303690529 27.734465317919078 28.007725655847043 22.32097598932859 58-59 21.93261050144495 27.59588332027575 28.172824338587464 22.298681839691834 60-61 21.934721477391594 27.69296867170264 28.139829825937152 22.232480024968616 62-63 21.908411090814823 27.649237717164244 28.072585555553697 22.36976563646724 64-65 21.714903371289065 27.539140261144023 28.066488323854283 22.679468043712625 66-67 21.8290765571204 27.575412658834054 28.05334557196334 22.54216521208221 68-69 21.854362738172924 27.521431919039706 28.090249513955694 22.53395582883168 70-71 21.752589363812348 27.43677494568005 27.95217579474778 22.858459895759825 72-73 21.816774210533676 27.503888061136145 27.89764423671553 22.78169349161465 74-75 21.774850857655984 27.759129932760697 27.749713739488797 22.71630547009453 76-77 21.760768231348386 27.51772387291001 27.831218737725138 22.890289158016465 78-79 21.71321895103032 27.52506905471583 27.752906862883016 23.00880513137084 80-81 21.81596619375277 27.688489021352826 27.62602886899969 22.869515915894716 82-83 21.905536376228106 27.511642178982708 27.812030346614886 22.7707910981743 84-85 21.85659398168693 27.605960788225897 27.59091541218502 22.946529817902153 86-87 21.662354549204714 27.642189210481938 27.63322732051168 23.062228919801676 88-89 21.754689477839378 27.405466169160814 27.546492319091787 23.293352033908025 90-91 21.69539400774657 27.426762256570914 27.630907593234916 23.246936142447602 92-93 21.856978449066577 27.19287253187615 27.671833958576293 23.27831506048098 94-95 21.584275730369715 27.170464394261966 27.76306748004776 23.48219239532056 96-97 21.643850664259094 26.975357187163723 27.641091867177693 23.73970028139949 98-99 21.420634344825103 27.064064539673687 27.591976072010898 23.92332504349031 100-101 21.691352896046897 27.060679481055228 27.465994128400073 23.781973494497798 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 28318.0 1 26547.5 2 17788.0 3 12811.5 4 17052.5 5 20016.0 6 20368.0 7 19061.0 8 17170.0 9 15189.0 10 13192.0 11 11460.5 12 9935.0 13 8500.0 14 7396.0 15 6684.0 16 6277.0 17 6105.0 18 6158.0 19 6481.0 20 7018.5 21 7782.0 22 8851.0 23 10253.5 24 12019.0 25 14193.5 26 16785.0 27 19860.5 28 23312.5 29 27518.5 30 31950.5 31 35853.0 32 39739.0 33 43172.0 34 45940.5 35 48679.0 36 52075.5 37 55168.0 38 56877.0 39 57631.5 40 57825.0 41 58444.5 42 59519.0 43 60921.5 44 62006.0 45 62894.0 46 63731.0 47 63778.5 48 63353.5 49 62182.0 50 60133.5 51 57767.0 52 55426.5 53 52692.5 54 49369.5 55 45153.5 56 40296.0 57 35186.0 58 30289.5 59 25920.5 60 21631.5 61 17645.5 62 14109.5 63 10841.0 64 8064.5 65 5863.5 66 4220.5 67 3184.5 68 2459.0 69 1835.0 70 1353.5 71 1020.5 72 757.0 73 549.5 74 412.5 75 296.5 76 212.5 77 147.5 78 88.5 79 50.5 80 30.0 81 15.5 82 9.0 83 8.0 84 4.5 85 3.0 86 2.0 87 1.0 88 0.5 89 1.0 90 1.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 13.925666796975975 2 20.63638356847051 3 16.456741152251464 4 21.634353391974155 5 21.42912849560439 6 15.458133679691223 7 14.288636259962653 8 11.501766533136413 9 2.8782006917020766 10-11 6.188531244068025 12-13 3.390895058170761 14-15 7.079204351317163 16-17 10.968324047364572 18-19 13.949946511054137 20-21 14.350390118597819 22-23 12.308392589733163 24-25 14.764665805676941 26-27 12.425057842126924 28-29 12.398840655915249 30-31 13.177557303803969 32-33 11.718174812960363 34-35 18.58818122378588 36-37 23.245054908941263 38-39 22.382732659747028 40-41 17.444067141502664 42-43 17.359235292011384 44-45 22.65468998238617 46-47 12.160041084219216 48-49 20.911725336102364 50-51 20.51527929764428 52-53 10.640302657672219 54-55 12.211862332549678 56-57 11.749370566806247 58-59 12.725562222530693 60-61 12.149201050257046 62-63 12.112879578994663 64-65 13.144988151990027 66-67 11.507873249101527 68-69 12.505328048367154 70-71 11.203027950120148 72-73 10.571706334160478 74-75 10.663871147802636 76-77 10.715152846931362 78-79 11.087539895984724 80-81 11.064829779584342 82-83 9.644319356229513 84-85 9.531308323429336 86-87 9.692731634603547 88-89 11.030887229802344 90-91 8.625454263640417 92-93 10.561160599762893 94-95 10.666029344609584 96-97 10.249276636195216 98-99 10.083291681769957 100-101 10.205450526035948 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2038739.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 96.13515042748207 #Duplication Level Percentage of deduplicated Percentage of total 1 98.42527922174436 94.62129023849319 2 1.1038791981000404 2.1224318552623136 3 0.23221752244137783 0.6697279935539717 4 0.08470697884349064 0.3257327261350606 5 0.04063002323749575 0.1952986697904373 6 0.029074717797747937 0.16770614214738527 7 0.01617029061865199 0.10881733247561481 8 0.011441573720257477 0.08799499285792627 9 0.00972070795599573 0.08410515494501443 >10 0.04200708874802433 0.6957366759606263 >50 0.002920087810011978 0.19868498741832025 >100 0.0017985938537216908 0.3206339633653805 >500 0.0 0.0 >1k 1.5399512877878332E-4 0.40183926759475974 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 3787 0.18575207517980477 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2518 0.1235077172703323 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 4.9049927430632363E-5 0.0 6 0.0 0.0 0.0 4.9049927430632363E-5 4.9049927430632363E-5 7 0.0 0.0 0.0 9.809985486126473E-5 4.9049927430632363E-5 8 0.0 0.0 0.0 9.809985486126473E-5 4.9049927430632363E-5 9 0.0 0.0 0.0 9.809985486126473E-5 4.9049927430632363E-5 10-11 0.0 0.0 0.0 9.809985486126473E-5 4.9049927430632363E-5 12-13 0.0 0.0 0.0 9.809985486126473E-5 1.2262481857658092E-4 14-15 0.0 0.0 0.0 9.809985486126473E-5 1.7167474600721328E-4 16-17 0.0 0.0 0.0 1.2262481857658092E-4 1.9619970972252945E-4 18-19 0.0 0.0 0.0 1.471497822918971E-4 2.4524963715316184E-4 20-21 0.0 0.0 0.0 1.7167474600721328E-4 2.942995645837942E-4 22-23 0.0 0.0 0.0 3.9239941944505896E-4 3.4334949201442655E-4 24-25 0.0 0.0 0.0 4.659743105910075E-4 3.4334949201442655E-4 26-27 4.9049927430632363E-5 0.0 0.0 8.583737300360664E-4 3.678744557297427E-4 28-29 4.9049927430632363E-5 0.0 0.0 0.001741272423787449 3.923994194450589E-4 30-31 4.9049927430632363E-5 0.0 0.0 0.006278390711120943 3.923994194450589E-4 32-33 4.9049927430632363E-5 0.0 0.0 0.016039326269816783 3.923994194450589E-4 34-35 4.9049927430632363E-5 2.4524963715316182E-5 0.0 0.030509054861853332 3.923994194450589E-4 36-37 4.9049927430632363E-5 4.9049927430632363E-5 0.0 0.05226269767733879 4.414493468756913E-4 38-39 4.9049927430632363E-5 4.9049927430632363E-5 0.0 0.08331130174092907 4.414493468756913E-4 40-41 4.9049927430632363E-5 4.9049927430632363E-5 0.0 0.11627285297431403 4.414493468756913E-4 42-43 4.9049927430632363E-5 4.9049927430632363E-5 0.0 0.15286409883756577 4.414493468756913E-4 44-45 4.9049927430632363E-5 4.9049927430632363E-5 0.0 0.19772025747287908 4.414493468756913E-4 46-47 4.9049927430632363E-5 4.9049927430632363E-5 0.0 0.2509149037713999 4.414493468756913E-4 48-49 4.9049927430632363E-5 4.9049927430632363E-5 0.0 0.3063658467317298 4.414493468756913E-4 50-51 4.9049927430632363E-5 4.9049927430632363E-5 0.0 0.37246062394450685 4.904992743063237E-4 52-53 4.9049927430632363E-5 4.9049927430632363E-5 0.0 0.45743962321807746 6.376490565982208E-4 54-55 4.9049927430632363E-5 4.9049927430632363E-5 0.0 0.553896305510416 6.376490565982208E-4 56-57 4.9049927430632363E-5 4.9049927430632363E-5 0.0 0.6646019917213533 6.866989840288531E-4 58-59 4.9049927430632363E-5 4.9049927430632363E-5 0.0 0.7836706905592132 7.602738751748017E-4 60-61 4.9049927430632363E-5 4.9049927430632363E-5 2.4524963715316182E-5 0.9105873777859745 8.338487663207502E-4 62-63 4.9049927430632363E-5 4.9049927430632363E-5 4.9049927430632363E-5 1.0407904101505883 8.338487663207502E-4 64-65 4.9049927430632363E-5 4.9049927430632363E-5 4.9049927430632363E-5 1.1830842496268525 8.828986937513826E-4 66-67 4.9049927430632363E-5 4.9049927430632363E-5 4.9049927430632363E-5 1.3427662883772764 9.074236574666988E-4 68-69 4.9049927430632363E-5 4.9049927430632363E-5 4.9049927430632363E-5 1.5176292796674806 9.319486211820149E-4 70-71 4.9049927430632363E-5 4.9049927430632363E-5 4.9049927430632363E-5 1.705269777053365 9.319486211820149E-4 72-73 4.9049927430632363E-5 4.9049927430632363E-5 4.9049927430632363E-5 1.9000225139166906 9.809985486126474E-4 74-75 4.9049927430632363E-5 4.9049927430632363E-5 4.9049927430632363E-5 2.1047078610847194 0.0010300484760432797 76-77 4.9049927430632363E-5 4.9049927430632363E-5 4.9049927430632363E-5 2.3250891850305506 0.0010300484760432797 78-79 4.9049927430632363E-5 4.9049927430632363E-5 4.9049927430632363E-5 2.5745325909790315 0.0010300484760432797 80-81 4.9049927430632363E-5 4.9049927430632363E-5 4.9049927430632363E-5 2.848819785171128 0.001079098403473912 82-83 4.9049927430632363E-5 4.9049927430632363E-5 4.9049927430632363E-5 3.135737335676612 0.0012507731494811253 84-85 4.9049927430632363E-5 9.809985486126473E-5 4.9049927430632363E-5 3.4241263840050147 0.0012752981131964416 86-87 4.9049927430632363E-5 9.809985486126473E-5 4.9049927430632363E-5 3.732527802725116 0.0013488730043423902 88-89 4.9049927430632363E-5 9.809985486126473E-5 4.9049927430632363E-5 4.0633695632447315 0.0014224478954883387 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2255 0.0 61.506866 1 GTATCAA 4415 0.0 46.439823 1 TATCAAC 5550 0.0 36.657917 2 ATCAACG 5430 0.0 36.439728 3 TCAACGC 5430 0.0 35.786507 4 CAACGCA 5560 0.0 34.642258 5 AACGCAG 6030 0.0 31.172306 6 GTGGTAT 955 0.0 30.520082 1 GTACATG 5290 0.0 29.258776 1 TACATGG 5425 0.0 27.594482 2 ACGCAGA 6910 0.0 26.822536 7 CGCAGAG 7065 0.0 25.849068 8 ACATGGG 5780 0.0 24.849457 3 CATGGGG 3695 0.0 24.46055 4 TGGTATC 1090 0.0 23.965284 2 GAGTACA 3955 0.0 22.871101 1 GCAGAGT 7405 0.0 22.855652 9 GAGTACT 4900 0.0 21.295752 12-13 AGTACAT 4200 0.0 20.811611 2 CAGAGTA 7205 0.0 20.792303 10-11 >>END_MODULE