FastQCFastQC Report
Sat 18 Jun 2016
SRR1930911_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1930911_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2038739
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64680.31725493062133014No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51260.2514299280094215No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36180.1774626374440279No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA28350.058.6253551
GTATCAA59550.046.968211
TCAACGC79350.034.9969524
TATCAAC79050.034.9521872
ATCAACG79150.034.8480453
CAACGCA79600.034.7498555
AACGCAG83650.033.180036
ACGCAGA96750.028.4422477
CGCAGAG98650.027.8463558
GTGGTAT12350.027.2999531
GCAGAGT108500.024.6187259
GTACATG72400.023.8744471
TGGTATC14050.022.6387122
TACATGG75400.022.4146462
ACATGGG76150.021.4457763
GTACTAG5100.021.4154991
AGAGTAC103100.021.05325710-11
TACACCG3451.8189894E-1220.6285975
CAGAGTA106700.019.94274310-11
GAGTACT71550.019.46190512-13
Produced by FastQC (version 0.11.5)