FastQCFastQC Report
Sat 18 Jun 2016
SRR1930910_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1930910_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3049396
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN55780.18292147035019393No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA23900.051.937281
GTATCAA46100.045.0960271
TCAACGC55250.037.5113754
ATCAACG56250.036.227413
CAACGCA58000.034.8847245
TATCAAC59500.034.082832
AACGCAG61800.032.8910146
ACGCAGA71650.027.811217
CGCAGAG75000.027.0129348
GTACATG59800.025.1453151
TACATGG60600.023.5581052
GCAGAGT82650.022.94549
ACATGGG64750.022.2794633
CATGGGG49450.019.6808764
AGAGTAC72400.019.35236410-11
GAGTACT50550.019.32546412-13
CAGAGTA77950.018.9667710-11
GAGTACA45150.018.440311
GTACTAG7000.017.3003621
GTACTTT57250.016.93126914-15
Produced by FastQC (version 0.11.5)