##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1930910_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3049396 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 22.37983620362852 30.0 10.0 31.0 2.0 33.0 2 21.297801269497302 28.0 10.0 31.0 2.0 33.0 3 21.725600086049827 28.0 10.0 31.0 2.0 33.0 4 23.623757622821042 32.0 2.0 35.0 2.0 37.0 5 23.602856762453943 32.0 2.0 35.0 2.0 37.0 6 24.320637595117198 32.0 10.0 35.0 2.0 37.0 7 24.382225201318555 32.0 10.0 35.0 2.0 37.0 8 25.11719009272656 32.0 17.0 35.0 2.0 37.0 9 26.207028539422232 33.0 17.0 37.0 2.0 39.0 10-11 26.42689273547942 33.0 17.0 37.0 2.0 39.0 12-13 26.33113131256157 33.0 16.5 37.0 2.0 39.0 14-15 26.512654309246813 33.0 13.5 38.0 2.0 40.0 16-17 25.638825196858654 32.5 10.0 38.0 2.0 40.0 18-19 25.034659978566246 32.0 10.0 38.0 2.0 40.0 20-21 24.52447287921936 32.0 5.0 38.0 2.0 40.0 22-23 24.432232317481887 31.0 9.0 37.5 2.0 40.0 24-25 24.10536283250847 31.0 5.0 37.0 2.0 39.5 26-27 23.75644767029274 30.5 2.0 37.0 2.0 39.0 28-29 23.656399496818388 30.0 2.0 37.0 2.0 39.0 30-31 23.532317219541184 30.0 2.0 37.0 2.0 39.0 32-33 23.19985433180866 30.0 2.0 37.0 2.0 39.0 34-35 20.374651898277563 24.5 2.0 35.5 2.0 39.0 36-37 18.78768336418097 17.0 2.0 34.0 2.0 38.5 38-39 17.88015708684605 15.5 2.0 33.0 2.0 38.0 40-41 19.092033635513395 19.5 2.0 34.0 2.0 38.0 42-43 18.983251273366925 19.5 2.0 34.0 2.0 38.0 44-45 17.10968942702096 12.0 2.0 33.0 2.0 38.0 46-47 17.876261397339015 17.5 2.0 32.5 2.0 38.0 48-49 17.283822107722315 14.0 2.0 33.0 2.0 38.0 50-51 15.4477391588367 9.5 2.0 30.5 2.0 37.0 52-53 18.184257964528058 18.5 2.0 32.5 2.0 37.5 54-55 19.697997570666452 24.5 2.0 34.5 2.0 38.0 56-57 19.88069522620217 25.5 2.0 35.0 2.0 39.0 58-59 19.509950495114442 24.0 2.0 35.0 2.0 39.0 60-61 19.17149363349332 23.0 2.0 34.5 2.0 38.0 62-63 18.689801193416663 22.0 2.0 34.0 2.0 38.0 64-65 18.191979165710194 20.5 2.0 34.0 2.0 38.0 66-67 17.76343184027263 18.5 2.0 33.5 2.0 37.0 68-69 17.298103624455464 16.0 2.0 33.0 2.0 37.0 70-71 16.72264179529323 10.5 2.0 32.0 2.0 36.0 72-73 16.24306354438715 7.5 2.0 32.0 2.0 36.0 74-75 15.707684407010436 4.5 2.0 31.0 2.0 35.0 76-77 15.1493038293485 2.0 2.0 31.0 2.0 35.0 78-79 14.655025290254201 2.0 2.0 30.5 2.0 34.0 80-81 14.149736537989817 2.0 2.0 30.0 2.0 34.0 82-83 13.67591237740195 2.0 2.0 29.5 2.0 34.0 84-85 13.160374218369803 2.0 2.0 29.0 2.0 34.0 86-87 12.614903410380286 2.0 2.0 29.0 2.0 34.0 88-89 12.077989706814071 2.0 2.0 28.0 2.0 34.0 90-91 11.519515012153226 2.0 2.0 26.5 2.0 33.5 92-93 10.924136779873784 2.0 2.0 25.5 2.0 33.0 94-95 10.294320416239806 2.0 2.0 24.5 2.0 32.5 96-97 9.602283370214955 2.0 2.0 21.5 2.0 32.0 98-99 8.854911759574684 2.0 2.0 9.5 2.0 32.0 100-101 7.905579334399336 2.0 2.0 2.0 2.0 31.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 6.789350780453422 1101 2 7.831744296063047 1101 3 7.52293259234132 1101 4 9.293422758535915 1101 5 9.233600408738742 1101 6 8.49085916330332 1101 7 8.245860443250045 1101 8 7.568001074597671 1101 9 7.8512013662830675 1101 10-11 7.2959935956412885 1101 12-13 6.888756849286747 1101 14-15 7.324696065365757 1101 16-17 8.160190337544226 1101 18-19 8.807680313432133 1101 20-21 9.156568506575134 1101 22-23 8.8979579473177 1101 24-25 9.084531949494927 1101 26-27 8.970822726017417 1101 28-29 8.778742404569936 1101 30-31 8.7000933790755 1101 32-33 8.6915403999033 1101 34-35 10.992138930205943 1101 36-37 5.451883522628965 1101 38-39 8.418219636242558 1101 40-41 10.000318787760143 1101 42-43 10.210604170887628 1101 44-45 4.803983482456996 1101 46-47 6.258194981964756 1101 48-49 9.167964351650468 1101 50-51 -3.0960641577137995 1101 52-53 5.797563439506536 1101 54-55 8.54155096373227 1101 56-57 9.034907482301524 1101 58-59 9.196746168814862 1101 60-61 8.975393900338172 1101 62-63 9.09977336741246 1101 64-65 8.94752555234911 1101 66-67 8.872878950898166 1101 68-69 8.775427061610007 1101 70-71 8.64889497471788 1101 72-73 8.560303473246709 1101 74-75 8.620022701219042 1101 76-77 8.511938890326597 1101 78-79 8.546538541451474 1101 80-81 8.475361991033159 1101 82-83 8.140805217583331 1101 84-85 8.222389568506669 1101 86-87 8.057080196486622 1101 88-89 7.7417003111746165 1101 90-91 7.640647896154334 1101 92-93 7.49430210138093 1101 94-95 7.068434158792533 1101 96-97 7.097635996682927 1101 98-99 6.748109767753766 1101 100-101 6.312618986459996 1102 1 6.5792402731354755 1102 2 7.550293052025079 1102 3 7.290324884071687 1102 4 9.07753980636716 1102 5 8.804514694492173 1102 6 8.203606587642241 1102 7 7.920108879082704 1102 8 7.333341149547994 1102 9 7.158736680647646 1102 10-11 6.7444779192580775 1102 12-13 6.586095521230522 1102 14-15 6.86424451145151 1102 16-17 7.597748439347601 1102 18-19 7.968690044278201 1102 20-21 8.479394799794825 1102 22-23 8.18443731656761 1102 24-25 8.193050761405967 1102 26-27 8.288162930216068 1102 28-29 7.961926786439822 1102 30-31 7.8228146066514235 1102 32-33 7.734562623079533 1102 34-35 10.047598564424053 1102 36-37 1.1750841907012237 1102 38-39 3.4572071879847037 1102 40-41 7.870270653034979 1102 42-43 8.9588557790131 1102 44-45 1.3606030404688276 1102 46-47 1.9868311015738058 1102 48-49 7.439709754372032 1102 50-51 -4.123958275476378 1102 52-53 4.738342587687001 1102 54-55 7.508568777850801 1102 56-57 8.024100518328694 1102 58-59 8.236147954588276 1102 60-61 8.125135221004864 1102 62-63 8.254402489800349 1102 64-65 8.144298077808866 1102 66-67 8.204564748676603 1102 68-69 8.017858727266965 1102 70-71 7.764495441655999 1102 72-73 7.725477866002226 1102 74-75 7.672006217718874 1102 76-77 7.7008060310408055 1102 78-79 7.79963450696272 1102 80-81 7.697875599214806 1102 82-83 7.443082853946592 1102 84-85 7.363743563455939 1102 86-87 7.134085994621289 1102 88-89 7.185305567608088 1102 90-91 7.012747315975506 1102 92-93 6.802950926602717 1102 94-95 6.369050691571482 1102 96-97 6.02811792395357 1102 98-99 5.722185088471154 1102 100-101 5.237170676313339 1103 1 6.811995084999289 1103 2 7.629392641822648 1103 3 7.252457367749482 1103 4 8.938294845817278 1103 5 8.827349260037582 1103 6 8.12647277455406 1103 7 8.095273304717239 1103 8 7.242281461757013 1103 9 7.236405430969054 1103 10-11 6.798116838266903 1103 12-13 6.636942842550656 1103 14-15 7.108327267521659 1103 16-17 7.678632776267239 1103 18-19 7.961619112848421 1103 20-21 8.358317189582323 1103 22-23 8.225753968991896 1103 24-25 8.19324059902095 1103 26-27 8.08488404617676 1103 28-29 8.257951411715691 1103 30-31 8.058163738267428 1103 32-33 7.959193971561216 1103 34-35 10.021778077498777 1103 36-37 -2.8614905697587183 1103 38-39 -1.5553965525275153 1103 40-41 6.521695036938063 1103 42-43 9.009098957168316 1103 44-45 1.1300953698256002 1103 46-47 1.052189074042424 1103 48-49 7.371358639035012 1103 50-51 -4.656396600134807 1103 52-53 4.4809048921734025 1103 54-55 7.516799359979366 1103 56-57 8.102319688317063 1103 58-59 8.297707424093353 1103 60-61 8.147444423260158 1103 62-63 8.090032602969828 1103 64-65 8.114052787915337 1103 66-67 8.000644082198864 1103 68-69 7.793598683653553 1103 70-71 7.853760042466337 1103 72-73 8.041208018671057 1103 74-75 7.722629782311259 1103 76-77 7.754870087832341 1103 78-79 7.488909283506887 1103 80-81 7.322557388699662 1103 82-83 7.131947209226322 1103 84-85 6.877897133880223 1103 86-87 6.497992447896619 1103 88-89 6.448647212947657 1103 90-91 6.460975644124321 1103 92-93 6.377219614119062 1103 94-95 6.0825033399429795 1103 96-97 5.772621769396208 1103 98-99 5.607944531935106 1103 100-101 5.139268299896535 1104 1 6.3692559329174685 1104 2 7.298819318165666 1104 3 6.8620972894385694 1104 4 8.700974589619069 1104 5 8.678311058150694 1104 6 7.904533904324019 1104 7 7.719654749756142 1104 8 6.915955745735346 1104 9 7.0865988876195765 1104 10-11 6.530066720969298 1104 12-13 6.256356544429099 1104 14-15 6.740836107034923 1104 16-17 7.418481681770945 1104 18-19 7.84222038962012 1104 20-21 8.160013785420375 1104 22-23 7.953148232379846 1104 24-25 8.096026522465277 1104 26-27 8.170951158968105 1104 28-29 7.939106265642206 1104 30-31 7.6137619560190295 1104 32-33 7.515330787817479 1104 34-35 9.919721150189535 1104 36-37 -3.0277668773315867 1104 38-39 -1.7141811089063133 1104 40-41 5.9336146669865 1104 42-43 8.09623588312737 1104 44-45 0.5876644403898119 1104 46-47 0.08506267533185508 1104 48-49 6.466201447595207 1104 50-51 -4.8486174065347 1104 52-53 3.5237674598937794 1104 54-55 6.28009238456967 1104 56-57 6.994019617432183 1104 58-59 7.212069734434106 1104 60-61 7.022214469335328 1104 62-63 7.087123580337298 1104 64-65 7.1549839536340585 1104 66-67 6.932691853544494 1104 68-69 6.745527765019265 1104 70-71 6.658492663012826 1104 72-73 6.727533565919206 1104 74-75 6.474711076297858 1104 76-77 6.489155690606978 1104 78-79 6.255981885657066 1104 80-81 6.1269465551272315 1104 82-83 5.963871986867204 1104 84-85 5.812386636167103 1104 86-87 5.610003532097727 1104 88-89 5.349703418750087 1104 90-91 5.159102152537681 1104 92-93 5.1087149686278455 1104 94-95 4.90684617803854 1104 96-97 4.476332571745536 1104 98-99 4.296551158827478 1104 100-101 3.8912088789584898 1105 1 5.838553132827016 1105 2 6.995079637436277 1105 3 6.601959497066062 1105 4 8.489497750011424 1105 5 8.361906203546305 1105 6 7.474881916502316 1105 7 7.537333024475689 1105 8 6.434602942203771 1105 9 6.692280057797827 1105 10-11 6.095784170409377 1105 12-13 5.9727000118678895 1105 14-15 6.398671481281561 1105 16-17 6.984910706163593 1105 18-19 7.401307431172494 1105 20-21 7.830412840914207 1105 22-23 7.533783357063186 1105 24-25 7.562853180840477 1105 26-27 7.674864411549766 1105 28-29 7.403593575274396 1105 30-31 7.105623701552947 1105 32-33 6.899496258284458 1105 34-35 9.193821706340458 1105 36-37 -3.4761408241023393 1105 38-39 -2.2333615769317294 1105 40-41 5.221054078652887 1105 42-43 7.4268769478434145 1105 44-45 -3.1815824945533127 1105 46-47 -4.098062900236831 1105 48-49 4.482723853398575 1105 50-51 -9.219281592315982 1105 52-53 0.45676552958797956 1105 54-55 4.9704941929614215 1105 56-57 5.8235998742382336 1105 58-59 6.239334996631246 1105 60-61 6.153324509875027 1105 62-63 6.188960115553087 1105 64-65 6.184145212822674 1105 66-67 6.034185505492989 1105 68-69 5.918313075031623 1105 70-71 5.875956236274323 1105 72-73 5.619030470547678 1105 74-75 5.592495677602862 1105 76-77 5.441699847581148 1105 78-79 5.44511242463515 1105 80-81 5.353089130659869 1105 82-83 5.186650660429226 1105 84-85 5.112589129833799 1105 86-87 5.046026409826455 1105 88-89 4.936602512751721 1105 90-91 4.554357971311788 1105 92-93 4.143452382313024 1105 94-95 3.931412309856471 1105 96-97 3.587356186857635 1105 98-99 3.5066793037675534 1105 100-101 3.1367437154068574 1106 1 6.009045963390324 1106 2 6.999232031531747 1106 3 6.642500053464573 1106 4 8.075129656733054 1106 5 8.033394153565023 1106 6 7.250245434343469 1106 7 7.141084202366201 1106 8 6.435647348235978 1106 9 6.459678512780993 1106 10-11 5.938583460213277 1106 12-13 5.7820146468127795 1106 14-15 6.160218915504515 1106 16-17 6.517088093247455 1106 18-19 6.80468696480413 1106 20-21 7.379443596911621 1106 22-23 7.0712279536701566 1106 24-25 7.190898827251534 1106 26-27 7.243084074781628 1106 28-29 7.260933872096938 1106 30-31 6.806870475922516 1106 32-33 6.716503119680045 1106 34-35 9.193823466575342 1106 36-37 0.14179667347733727 1106 38-39 1.0692069578135452 1106 40-41 6.299958661013694 1106 42-43 7.352583594138295 1106 44-45 -3.37102777413142 1106 46-47 -4.154379268426336 1106 48-49 4.328419609706966 1106 50-51 -9.155207282240045 1106 52-53 0.11127278703641252 1106 54-55 4.544869397588727 1106 56-57 5.57679880777059 1106 58-59 5.845521166113702 1106 60-61 5.823156372238742 1106 62-63 5.921472475335712 1106 64-65 5.775528980405955 1106 66-67 5.636764073577115 1106 68-69 5.4313769516086445 1106 70-71 5.306215730101176 1106 72-73 5.288446037360917 1106 74-75 5.213422640763493 1106 76-77 5.104947844727221 1106 78-79 4.874158236508288 1106 80-81 4.472327441820111 1106 82-83 4.461850476426008 1106 84-85 4.292062385411688 1106 86-87 4.085328347610366 1106 88-89 3.759643165963153 1106 90-91 3.6390936251040973 1106 92-93 3.5526242622086777 1106 94-95 3.43316286345034 1106 96-97 3.3378431851760233 1106 98-99 2.980538348851862 1106 100-101 2.8111876865436995 1107 1 5.966825148105642 1107 2 7.14630130495091 1107 3 6.802410080699502 1107 4 8.010949262407646 1107 5 8.405663147555707 1107 6 6.780036891472168 1107 7 7.0575855761338495 1107 8 6.520562484524149 1107 9 6.663519051549027 1107 10-11 6.1404438234435865 1107 12-13 5.868698584082953 1107 14-15 6.116281391545392 1107 16-17 6.884111792575997 1107 18-19 7.024738228977579 1107 20-21 7.346074466813086 1107 22-23 7.45264014941435 1107 24-25 7.4199847675377235 1107 26-27 7.426095036873122 1107 28-29 7.483609787151753 1107 30-31 7.155287871547561 1107 32-33 7.028333015588924 1107 34-35 9.12832173083294 1107 36-37 0.16045273201306287 1107 38-39 1.1806096968003637 1107 40-41 6.777888886706954 1107 42-43 7.891773705682617 1107 44-45 -3.0284988128119625 1107 46-47 -3.98325370058679 1107 48-49 4.666424554469081 1107 50-51 -9.080413748097545 1107 52-53 0.48635576741097 1107 54-55 5.085517091969837 1107 56-57 6.235969524948093 1107 58-59 6.41386994681978 1107 60-61 6.5328205638529475 1107 62-63 6.3042908294592195 1107 64-65 6.267556853797604 1107 66-67 6.349858343289711 1107 68-69 6.23938440748713 1107 70-71 6.29749227213366 1107 72-73 6.029960334027944 1107 74-75 5.939555681845626 1107 76-77 5.9333651334962525 1107 78-79 5.884648825832569 1107 80-81 5.704479815826849 1107 82-83 5.459884659665374 1107 84-85 5.339848395555 1107 86-87 5.047423847502843 1107 88-89 4.810305606474406 1107 90-91 4.75077485758138 1107 92-93 4.438665994825541 1107 94-95 4.418640797015284 1107 96-97 4.1562410528642335 1107 98-99 3.660463509569988 1107 100-101 3.254411412319107 1108 1 6.180847431527429 1108 2 6.3945715123730835 1108 3 6.696553408500403 1108 4 8.126689753566652 1108 5 8.26603962811172 1108 6 7.322898794437087 1108 7 7.613588817595193 1108 8 6.671208520998427 1108 9 4.87042009904037 1108 10-11 5.788827900672796 1108 12-13 5.368793021562478 1108 14-15 6.165459183291247 1108 16-17 6.266152402986219 1108 18-19 6.934217993166811 1108 20-21 7.5485618525274845 1108 22-23 7.649142559864103 1108 24-25 7.424909041678635 1108 26-27 7.76384743250728 1108 28-29 7.443569920771228 1108 30-31 7.216441874607032 1108 32-33 7.44655073183068 1108 34-35 9.572463347671412 1108 36-37 -3.2706340980877933 1108 38-39 -2.0342554984768437 1108 40-41 5.550010388362285 1108 42-43 7.76172278208789 1108 44-45 -2.9079952985524766 1108 46-47 -3.7484759135054553 1108 48-49 5.1487265597935234 1108 50-51 -9.166401080475797 1108 52-53 0.8534234492614239 1108 54-55 5.679055312576551 1108 56-57 6.761378655255378 1108 58-59 7.003442462378068 1108 60-61 6.955784719285571 1108 62-63 6.991427966825988 1108 64-65 6.908739547498623 1108 66-67 6.735926262954383 1108 68-69 6.789227323930607 1108 70-71 6.564386926934084 1108 72-73 6.5334457269968205 1108 74-75 6.570858332174225 1108 76-77 6.506726805720206 1108 78-79 6.421578133835245 1108 80-81 6.207172765552846 1108 82-83 6.178336902020238 1108 84-85 5.889104040870764 1108 86-87 5.867771740648555 1108 88-89 5.842835685958645 1108 90-91 5.609652429903477 1108 92-93 5.453625876101954 1108 94-95 5.37505063579483 1108 96-97 5.048098764257944 1108 98-99 4.854329078391396 1108 100-101 4.63611561310466 1109 1 4.902987481852232 1109 2 6.454311513781747 1109 3 6.022861044604543 1109 4 7.667673471147129 1109 5 7.47464685701992 1109 6 5.057386881575933 1109 7 4.686359901652786 1109 8 3.2956581176337387 1109 9 2.6068762482717602 1109 10-11 2.6015466978619806 1109 12-13 3.3964670202327625 1109 14-15 3.800147017799887 1109 16-17 2.9697399222488876 1109 18-19 3.7804852734524452 1109 20-21 4.184109396000544 1109 22-23 5.766015959156832 1109 24-25 5.553709524183763 1109 26-27 4.492395536348823 1109 28-29 4.418573539172673 1109 30-31 4.50201346980737 1109 32-33 4.329245059338195 1109 34-35 6.140210368391887 1109 36-37 -4.647368899060234 1109 38-39 -3.3058845415083447 1109 40-41 2.95456740701621 1109 42-43 3.5426440541862654 1109 44-45 -4.50024895894286 1109 46-47 -5.714956358270847 1109 48-49 1.8329181895602353 1109 50-51 -9.50115120179867 1109 52-53 -1.2090897569363754 1109 54-55 2.1007651020745968 1109 56-57 3.508474839718776 1109 58-59 4.309535231225297 1109 60-61 4.5923099083905825 1109 62-63 4.5039273186096835 1109 64-65 4.28917247084966 1109 66-67 4.49591759814065 1109 68-69 4.52176379625622 1109 70-71 4.371386037620017 1109 72-73 4.191500791713967 1109 74-75 4.300747082776969 1109 76-77 4.510483944806911 1109 78-79 4.454799799816934 1109 80-81 4.133142690638392 1109 82-83 4.111818299566849 1109 84-85 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-11.410469950061087 2313 84-85 -10.8939717083605 2313 86-87 -10.351315872367188 2313 88-89 -9.81413629531631 2313 90-91 -9.252561875479975 2313 92-93 -8.69473166669487 2313 94-95 -8.219125324552133 2313 96-97 -7.50141762015621 2313 98-99 -6.718102538479501 2313 100-101 -5.751271371153452 2314 1 -20.253477227704295 2314 2 -19.193408661108947 2314 3 -19.60487162247853 2314 4 -21.49753577307523 2314 5 -21.46320448589076 2314 6 -22.170595629354736 2314 7 -22.233065457497744 2314 8 -22.96293026462735 2314 9 -24.019294894763842 2314 10-11 -24.242925507009975 2314 12-13 -24.154750968524265 2314 14-15 -24.32571182042622 2314 16-17 -23.479510546208328 2314 18-19 -22.8830062943177 2314 20-21 -22.356177924424188 2314 22-23 -22.263496845340356 2314 24-25 -21.95659653452954 2314 26-27 -21.59426904828578 2314 28-29 -21.497260314668676 2314 30-31 -21.375875349680452 2314 32-33 -21.049112217363867 2314 34-35 -18.347765896442592 2314 36-37 -16.720448224481846 2314 38-39 -15.851324891289732 2314 40-41 -17.06120398586324 2314 42-43 -16.93096618941149 2314 44-45 -15.065832968936615 2314 46-47 -15.780384215859854 2314 48-49 -15.246719536860752 2314 50-51 -13.382943831405166 2314 52-53 -16.08245015668653 2314 54-55 -17.563819532528775 2314 56-57 -17.7271591390445 2314 58-59 -17.366136224115117 2314 60-61 -17.030894338954827 2314 62-63 -16.54791527976633 2314 64-65 -16.058422782117237 2314 66-67 -15.629160478061724 2314 68-69 -15.175456690939281 2314 70-71 -14.58988816600272 2314 72-73 -14.104720320091158 2314 74-75 -13.586979338209915 2314 76-77 -13.05213316949665 2314 78-79 -12.554413192063143 2314 80-81 -12.041123393059296 2314 82-83 -11.591179826604296 2314 84-85 -11.083014918237042 2314 86-87 -10.535883773601755 2314 88-89 -9.997932591612605 2314 90-91 -9.438210023628123 2314 92-93 -8.85291067904055 2314 94-95 -8.219125324552133 2314 96-97 -7.529658360896951 2314 98-99 -6.78554081008444 2314 100-101 -5.833370136585551 2315 1 -20.253477227704295 2315 2 -19.193408661108947 2315 3 -19.60487162247853 2315 4 -17.07008716364355 2315 5 -21.46320448589076 2315 6 -22.170595629354736 2315 7 -22.233065457497744 2315 8 -22.96293026462735 2315 9 -19.838521013264447 2315 10-11 -18.30081547073428 2315 12-13 -15.318445043494034 2315 14-15 -15.255881107004214 2315 16-17 -19.885798333390916 2315 18-19 -22.8830062943177 2315 20-21 -22.356177924424188 2315 22-23 -22.263496845340356 2315 24-25 -21.95659653452954 2315 26-27 -21.59426904828578 2315 28-29 -21.497260314668676 2315 30-31 -21.375875349680452 2315 32-33 -21.049112217363867 2315 34-35 -18.347765896442592 2315 36-37 -16.720448224481846 2315 38-39 -15.851324891289732 2315 40-41 -17.06120398586324 2315 42-43 -16.93096618941149 2315 44-45 -15.065832968936615 2315 46-47 -15.780384215859854 2315 48-49 -15.246719536860752 2315 50-51 -13.382943831405166 2315 52-53 -16.08245015668653 2315 54-55 -17.563819532528775 2315 56-57 -17.7271591390445 2315 58-59 -17.366136224115117 2315 60-61 -17.030894338954827 2315 62-63 -16.54791527976633 2315 64-65 -16.058422782117237 2315 66-67 -15.629160478061724 2315 68-69 -15.175456690939281 2315 70-71 -14.58988816600272 2315 72-73 -14.104720320091158 2315 74-75 -13.586979338209915 2315 76-77 -13.05213316949665 2315 78-79 -12.554413192063143 2315 80-81 -12.041123393059296 2315 82-83 -11.591179826604296 2315 84-85 -11.083014918237042 2315 86-87 -10.535883773601755 2315 88-89 -9.997932591612605 2315 90-91 -9.438210023628123 2315 92-93 -8.85291067904055 2315 94-95 -8.219125324552133 2315 96-97 -7.529658360896951 2315 98-99 -6.78554081008444 2315 100-101 -5.833370136585551 2316 1 -20.253477227704295 2316 2 -19.193408661108947 2316 3 -19.60487162247853 2316 4 -21.49753577307523 2316 5 -21.46320448589076 2316 6 -22.170595629354736 2316 7 -22.233065457497744 2316 8 -22.96293026462735 2316 9 -24.019294894763842 2316 10-11 -24.242925507009975 2316 12-13 -24.154750968524265 2316 14-15 -24.32571182042622 2316 16-17 -23.479510546208328 2316 18-19 -22.8830062943177 2316 20-21 -22.356177924424188 2316 22-23 -22.263496845340356 2316 24-25 -21.95659653452954 2316 26-27 -21.59426904828578 2316 28-29 -21.497260314668676 2316 30-31 -21.375875349680452 2316 32-33 -21.049112217363867 2316 34-35 -18.347765896442592 2316 36-37 -16.720448224481846 2316 38-39 -15.851324891289732 2316 40-41 -17.06120398586324 2316 42-43 -16.93096618941149 2316 44-45 -15.065832968936615 2316 46-47 -15.780384215859854 2316 48-49 -15.246719536860752 2316 50-51 -13.382943831405166 2316 52-53 -16.08245015668653 2316 54-55 -17.563819532528775 2316 56-57 -17.7271591390445 2316 58-59 -17.366136224115117 2316 60-61 -17.030894338954827 2316 62-63 -16.54791527976633 2316 64-65 -16.058422782117237 2316 66-67 -15.629160478061724 2316 68-69 -15.175456690939281 2316 70-71 -14.58988816600272 2316 72-73 -14.104720320091158 2316 74-75 -13.586979338209915 2316 76-77 -13.05213316949665 2316 78-79 -12.554413192063143 2316 80-81 -12.041123393059296 2316 82-83 -11.591179826604296 2316 84-85 -11.083014918237042 2316 86-87 -10.535883773601755 2316 88-89 -9.997932591612605 2316 90-91 -9.438210023628123 2316 92-93 -8.85291067904055 2316 94-95 -8.219125324552133 2316 96-97 -7.529658360896951 2316 98-99 -6.78554081008444 2316 100-101 -5.833370136585551 >>END_MODULE >>Per sequence quality scores fail #Quality Count 2 542555.0 3 34959.0 4 68179.0 5 44277.0 6 39805.0 7 49047.0 8 61866.0 9 71202.0 10 72475.0 11 67347.0 12 58953.0 13 53846.0 14 51597.0 15 54372.0 16 58790.0 17 61761.0 18 63528.0 19 66314.0 20 68991.0 21 73185.0 22 76928.0 23 79994.0 24 84644.0 25 88529.0 26 93357.0 27 98242.0 28 103348.0 29 107136.0 30 110386.0 31 108221.0 32 104384.0 33 98887.0 34 90188.0 35 73307.0 36 48312.0 37 18442.0 38 2030.0 39 12.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.30709895838343 19.957796735690998 13.96381752955094 23.771286776374637 2 15.485715005225451 20.483469530800775 40.78969348224816 23.241121981725616 3 18.783435176053786 25.582023784792284 29.97729930138839 25.657241737765542 4 12.729660354770992 16.85791390303357 38.47874273363948 31.933683008555963 5 13.440829396071358 37.417215590410954 35.399435174345655 13.74251983917203 6 30.907048754022814 37.17157736007442 18.018006334324866 13.903367551577903 7 26.894411982871652 32.34685873760741 22.833265870933133 17.925463408587802 8 25.08563892965745 36.05378356795319 20.751265031250632 18.10931247113873 9 25.99677311461469 16.285581387790916 21.002785439881485 36.714860057712905 10-11 24.84758178800703 26.29556578402888 28.736752306458257 20.12010012150583 12-13 25.236030185719514 24.015182049791186 28.792755046045205 21.956032718444092 14-15 22.437507921006137 25.43034360392733 26.52311407278344 25.609034402283097 16-17 22.299793218365423 28.36362189818254 27.072035999782884 22.264548883669157 18-19 22.17719927411658 27.50522673064144 28.869746480436763 21.447827514805216 20-21 22.838517098785385 26.723043816018855 28.93360962177935 21.50482946341641 22-23 22.653264363566393 26.57379337876628 28.64891613807109 22.124026119596238 24-25 22.501532336090694 26.790183541327096 28.590196244227684 22.11808787835453 26-27 22.36995997255347 26.9242262507726 28.580001558430162 22.12581221824377 28-29 22.391857361962032 27.10362580214602 28.54289197048148 21.961624865410474 30-31 22.579229633825797 26.93338348344922 28.413748394345806 22.07363848837918 32-33 22.505091458550368 27.089168567165018 28.284840123508932 22.120899850775686 34-35 22.801189692889675 27.01158272502635 28.331565214430377 21.855662367653597 36-37 23.29931379724345 26.972721918829194 27.88030905007454 21.847655233852812 38-39 23.06417576629019 27.025919339452447 27.900403955833593 22.00950093842377 40-41 23.037213550191886 26.854706608957013 28.10538644654852 22.002693394302582 42-43 23.14351315842132 26.939011668912045 27.811299194379906 22.10617597828673 44-45 23.42914866279654 27.098270531403045 27.484179145059688 21.988401660740724 46-47 22.78414466665132 26.99794658556164 27.83216271224308 22.385746035543956 48-49 23.306189068735275 27.142808540950973 27.331878956292666 22.219123434021082 50-51 23.419983846413455 27.32300799424907 27.15135785209542 22.10565030724205 52-53 22.546856546794274 27.37333478642193 27.638838537392196 22.44097012939161 54-55 22.359011708344752 27.314484715915537 27.80537806815428 22.521125507585428 56-57 22.490937934357984 27.495849976777897 27.53692295239385 22.476289136470267 58-59 22.5209132499236 27.36112188068729 27.709714056349465 22.40825081303965 60-61 22.498910450445926 27.435810950459043 27.6789942584655 22.386284340629526 62-63 22.458187331847892 27.415876068347846 27.628276743154434 22.49765985664983 64-65 22.299763548209892 27.27119348443956 27.64844634455086 22.780596622799685 66-67 22.378419588108745 27.4065873753991 27.493745955124478 22.721247081367675 68-69 22.408244102375054 27.350545019014323 27.541351926292627 22.699858952317996 70-71 22.35943615137005 27.18833871065837 27.474542747028913 22.97768239094266 72-73 22.516051252895892 27.307963680741643 27.376613227306485 22.799371839055983 74-75 22.491534598120182 27.41921107439306 27.304041211353468 22.78521311613329 76-77 22.526294625186825 27.241094438074224 27.378312818585727 22.854298118153224 78-79 22.397107450878647 27.374468173143647 27.27920669987678 22.949217676100925 80-81 22.51727364289699 27.42912524550874 27.14988525226058 22.903715859333694 82-83 22.684414508580545 27.268488154419924 27.240374441686388 22.806722895313143 84-85 22.582030886951532 27.390910281770438 27.100855710383055 22.926203120894982 86-87 22.480809791083722 27.409903595426965 27.097564506405288 23.011722107084022 88-89 22.509772281808246 27.300052819117987 27.002861470129634 23.18731342894413 90-91 22.58374926897622 27.294166140715564 26.999910974331655 23.12217361597656 92-93 22.588766455095097 27.172666955411835 27.05052737965243 23.188039209840642 94-95 22.448277798962508 27.142506776075027 27.035339031767887 23.37387639319458 96-97 22.45747472828643 27.05671450202598 26.96424059445931 23.52157017522828 98-99 22.353966385323044 27.106164654693394 26.85191697484846 23.6879519851351 100-101 22.815288899506026 27.200203642599863 26.644278514802522 23.34022894309159 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 37131.0 1 40510.0 2 30355.0 3 17646.0 4 22661.0 5 28810.0 6 30645.5 7 29332.5 8 26951.0 9 24384.0 10 21786.0 11 19196.0 12 16501.0 13 14067.5 14 12002.0 15 10340.5 16 9039.5 17 8255.5 18 8061.0 19 8204.5 20 8856.0 21 9874.0 22 11123.0 23 12959.5 24 15498.5 25 18542.5 26 22203.5 27 27052.5 28 32835.0 29 39116.5 30 45621.0 31 52115.0 32 58135.5 33 62720.5 34 66617.0 35 70708.0 36 74778.5 37 78371.5 38 81385.5 39 83481.5 40 85039.5 41 86791.0 42 88455.5 43 89981.5 44 91490.0 45 93045.5 46 94008.5 47 94219.5 48 93574.5 49 91850.5 50 89493.5 51 86774.0 52 83529.5 53 79666.5 54 75151.0 55 69363.5 56 62542.0 57 55922.0 58 49245.0 59 42688.5 60 36443.0 61 30344.5 62 24884.5 63 20019.5 64 15749.5 65 12173.0 66 9318.5 67 7160.5 68 5500.5 69 4133.5 70 3140.0 71 2367.0 72 1742.0 73 1260.0 74 913.5 75 686.5 76 496.0 77 334.0 78 225.0 79 141.0 80 78.5 81 52.5 82 29.5 83 18.0 84 17.5 85 10.0 86 5.0 87 4.0 88 2.5 89 3.0 90 2.0 91 0.0 92 0.5 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 14.759349064536059 2 21.86747801859778 3 17.60014114270498 4 22.860855067692093 5 22.76952550603464 6 16.55563921511014 7 15.337824277332299 8 12.367367176975375 9 3.130587172017016 10-11 6.685045169600799 12-13 3.6678739002740213 14-15 7.625083787084392 16-17 11.79256482267308 18-19 14.922299366825431 20-21 15.340562524513052 22-23 13.254395296642352 24-25 15.840940304243855 26-27 13.304601960519394 28-29 13.319818088565736 30-31 14.207748026166495 32-33 12.645815761547533 34-35 19.583107605571726 36-37 24.29882835814043 38-39 23.426393292311 40-41 18.509485157060613 42-43 18.46839505265961 44-45 23.775577196271 46-47 13.106300395225809 48-49 21.923587490768664 50-51 21.53667480379721 52-53 11.533234778297079 54-55 13.18889052127044 56-57 12.693054624587951 58-59 13.724947497799564 60-61 13.129337744261488 62-63 13.099364595480548 64-65 14.185874842099878 66-67 12.465534158239862 68-69 13.533794889217408 70-71 12.111086916884524 72-73 11.45967922827996 74-75 11.517280799214008 76-77 11.598788743738105 78-79 12.027939303389918 80-81 11.991981362866614 82-83 10.474582507486728 84-85 10.377858434916291 86-87 10.519820974383123 88-89 11.961778660429804 90-91 9.383759931474954 92-93 11.451661247014162 94-95 11.550566079315379 96-97 11.096000650620647 98-99 10.944364064227802 100-101 11.044826582051002 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3049396.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 97.79302531809802 #Duplication Level Percentage of deduplicated Percentage of total 1 99.14909679596244 96.96090133234104 2 0.596418541878071 1.166511471321306 3 0.12250136323124956 0.3593933674792533 4 0.04323179742433284 0.16911073040258667 5 0.0269099726382944 0.13158038177630246 6 0.013050921270306586 0.07657734445269576 7 0.012009612813235938 0.08221194589337355 8 0.005980204595162755 0.0467857839505726 9 0.005144278312304982 0.045276708531472525 >10 0.023216346666625098 0.4020738772810708 >50 0.0013920487092537698 0.09487953212645492 >100 9.138556622379609E-4 0.15161807809079736 >500 3.356520912866515E-5 0.026456493369782057 >1k 6.71304182572149E-5 0.10352826313666148 >5k 3.356520912860745E-5 0.18309468984663846 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 5578 0.18292147035019393 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 3.279337941021763E-5 0.0 0.0 0.0 3 0.0 3.279337941021763E-5 0.0 0.0 0.0 4 0.0 3.279337941021763E-5 0.0 0.0 0.0 5 0.0 6.558675882043526E-5 0.0 0.0 0.0 6 0.0 6.558675882043526E-5 0.0 0.0 3.279337941021763E-5 7 0.0 6.558675882043526E-5 0.0 0.0 3.279337941021763E-5 8 0.0 6.558675882043526E-5 0.0 0.0 6.558675882043526E-5 9 0.0 6.558675882043526E-5 0.0 0.0 6.558675882043526E-5 10-11 0.0 6.558675882043526E-5 0.0 1.6396689705108814E-5 6.558675882043526E-5 12-13 0.0 6.558675882043526E-5 0.0 3.279337941021763E-5 3.7712386321750273E-4 14-15 0.0 6.558675882043526E-5 0.0 3.279337941021763E-5 4.755040014481557E-4 16-17 0.0 6.558675882043526E-5 0.0 6.558675882043526E-5 4.919006911532645E-4 18-19 0.0 6.558675882043526E-5 0.0 6.558675882043526E-5 4.919006911532645E-4 20-21 0.0 6.558675882043526E-5 0.0 6.558675882043526E-5 6.558675882043526E-4 22-23 3.279337941021763E-5 6.558675882043526E-5 0.0 8.198344852554408E-5 6.886609676145702E-4 24-25 3.279337941021763E-5 6.558675882043526E-5 0.0 1.3117351764087052E-4 7.214543470247879E-4 26-27 3.279337941021763E-5 6.558675882043526E-5 0.0 2.2955365587152342E-4 7.378510367298967E-4 28-29 3.279337941021763E-5 6.558675882043526E-5 0.0 5.738841396788085E-4 7.542477264350055E-4 30-31 3.279337941021763E-5 8.198344852554408E-5 0.0 0.0022463464895999077 7.706444161401144E-4 32-33 3.279337941021763E-5 9.838013823065289E-5 0.0 0.006870212986440593 7.870411058452231E-4 34-35 3.279337941021763E-5 9.838013823065289E-5 0.0 0.013592855765535207 8.198344852554407E-4 36-37 3.279337941021763E-5 9.838013823065289E-5 0.0 0.02423430738415083 8.362311749605495E-4 38-39 3.279337941021763E-5 9.838013823065289E-5 0.0 0.03995873281135018 8.526278646656584E-4 40-41 6.558675882043526E-5 1.3117351764087052E-4 0.0 0.055994695342946606 8.526278646656584E-4 42-43 6.558675882043526E-5 1.3117351764087052E-4 0.0 0.07301445925684955 8.526278646656584E-4 44-45 6.558675882043526E-5 1.3117351764087052E-4 0.0 0.09500242015140048 8.690245543707672E-4 46-47 6.558675882043526E-5 1.3117351764087052E-4 0.0 0.12217173499276579 8.85421244075876E-4 48-49 6.558675882043526E-5 1.3117351764087052E-4 0.0 0.15132504928844925 9.182146234860937E-4 50-51 6.558675882043526E-5 1.3117351764087052E-4 0.0 0.18331499090311654 9.83801382306529E-4 52-53 6.558675882043526E-5 1.3117351764087052E-4 0.0 0.22752046634808992 0.0011313715896525082 54-55 6.558675882043526E-5 1.3117351764087052E-4 0.0 0.28026861712942497 0.0012133550381780522 56-57 6.558675882043526E-5 1.3117351764087052E-4 0.0 0.34031329482953343 0.0012297517278831611 58-59 6.558675882043526E-5 1.3117351764087052E-4 0.0 0.4051949959926491 0.00124614841758827 60-61 6.558675882043526E-5 1.3117351764087052E-4 0.0 0.47697970352161545 0.00124614841758827 62-63 6.558675882043526E-5 1.3117351764087052E-4 0.0 0.549075948154979 0.0014101153146393582 64-65 6.558675882043526E-5 1.3117351764087052E-4 0.0 0.6252057784557992 0.0014101153146393582 66-67 6.558675882043526E-5 1.3117351764087052E-4 0.0 0.7142070101751297 0.001426512004344467 68-69 9.838013823065289E-5 1.3117351764087052E-4 0.0 0.8157681062085738 0.0014429086940495757 70-71 9.838013823065289E-5 1.3117351764087052E-4 0.0 0.9234615641917285 0.0014757020734597933 72-73 1.3117351764087052E-4 1.3117351764087052E-4 0.0 1.036385566190813 0.0016232722808057726 74-75 1.3117351764087052E-4 1.3117351764087052E-4 0.0 1.1496211052942944 0.0017216524190364255 76-77 1.3117351764087052E-4 1.3117351764087052E-4 0.0 1.2780399790647066 0.0017872391778568607 78-79 1.3117351764087052E-4 1.4757020734597933E-4 0.0 1.423511410128432 0.0018364292469721874 80-81 1.3117351764087052E-4 1.6396689705108815E-4 0.0 1.583526704960589 0.0018856193160875136 82-83 1.3117351764087052E-4 1.6396689705108815E-4 0.0 1.7531504599599397 0.001967602764613058 84-85 1.3117351764087052E-4 1.6396689705108815E-4 0.0 1.9208721989534978 0.002033189523433493 86-87 1.3117351764087052E-4 1.6396689705108815E-4 0.0 2.0951526138290992 0.002115172971959037 88-89 1.3117351764087052E-4 1.6396689705108815E-4 0.0 2.2907815187007525 0.002197156420484581 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2390 0.0 51.93728 1 GTATCAA 4610 0.0 45.096027 1 TCAACGC 5525 0.0 37.511375 4 ATCAACG 5625 0.0 36.22741 3 CAACGCA 5800 0.0 34.884724 5 TATCAAC 5950 0.0 34.08283 2 AACGCAG 6180 0.0 32.891014 6 ACGCAGA 7165 0.0 27.81121 7 CGCAGAG 7500 0.0 27.012934 8 GTACATG 5980 0.0 25.145315 1 TACATGG 6060 0.0 23.558105 2 GCAGAGT 8265 0.0 22.9454 9 ACATGGG 6475 0.0 22.279463 3 CATGGGG 4945 0.0 19.680876 4 AGAGTAC 7240 0.0 19.352364 10-11 GAGTACT 5055 0.0 19.325464 12-13 CAGAGTA 7795 0.0 18.96677 10-11 GAGTACA 4515 0.0 18.44031 1 GTACTAG 700 0.0 17.300362 1 GTACTTT 5725 0.0 16.931269 14-15 >>END_MODULE