FastQCFastQC Report
Sat 18 Jun 2016
SRR1930910_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1930910_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3049396
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53740.17623162095050954No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46650.15298111494866526No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30570.10024936085703529No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA33750.057.8285331
GTATCAA65900.042.370741
ATCAACG82450.033.454913
CAACGCA83150.033.217835
TCAACGC83500.033.033134
TATCAAC87000.031.8705332
AACGCAG88050.031.5848486
ACGCAGA102000.026.8464097
CGCAGAG105150.025.9970328
GCAGAGT120400.022.3494649
GTACATG82600.021.6163271
TACATGG85900.020.3399732
ACATGGG84500.019.8893663
AGAGTAC109950.019.68249510-11
CAGAGTA117250.018.94277410-11
GAGTACT77250.018.73751312-13
TACCCCG2603.3150536E-718.2531245
TATACTG15950.017.8525855
GAGTACA67500.017.5174031
GTACTTT82550.017.47700714-15
Produced by FastQC (version 0.11.5)