##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1930910_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3049396 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.245262996344195 31.0 31.0 33.0 30.0 34.0 2 31.360671424767396 31.0 31.0 34.0 30.0 34.0 3 31.443620966250364 31.0 31.0 34.0 28.0 34.0 4 35.307240187892944 37.0 35.0 37.0 33.0 37.0 5 35.087923641271914 37.0 35.0 37.0 32.0 37.0 6 35.13172608608393 37.0 35.0 37.0 32.0 37.0 7 35.014036222255164 36.0 35.0 37.0 32.0 37.0 8 34.992190256693455 36.0 35.0 37.0 32.0 37.0 9 36.49445955854864 38.0 35.0 39.0 32.0 39.0 10-11 36.4240690615453 38.0 35.0 39.0 32.0 39.0 12-13 36.32168681929143 38.0 35.0 39.0 32.0 39.0 14-15 37.33196196886203 39.0 36.0 41.0 32.0 41.0 16-17 37.29728165840055 39.0 36.0 41.0 32.0 41.0 18-19 37.28360353984855 39.0 36.0 40.0 31.5 41.0 20-21 37.24957811317388 39.0 36.0 40.0 31.0 41.0 22-23 37.12079178958719 39.0 36.0 40.0 31.0 41.0 24-25 36.994068005598486 39.0 36.0 40.0 31.0 41.0 26-27 36.71511473091721 39.0 35.5 40.0 30.0 41.0 28-29 36.52747003013056 38.0 35.0 40.0 30.0 41.0 30-31 36.28889425971569 38.0 35.0 40.0 30.0 41.0 32-33 36.048095098176816 38.0 34.5 40.0 29.5 41.0 34-35 36.23983142891248 38.0 35.0 40.0 30.0 41.0 36-37 36.28941337891176 38.0 35.0 40.0 30.0 41.0 38-39 36.248568568988745 38.0 35.0 40.0 30.0 41.0 40-41 36.148641894985104 38.0 35.0 40.0 30.0 41.0 42-43 36.00820834683327 38.0 35.0 40.0 29.0 41.0 44-45 35.814493263584 38.0 34.5 40.0 28.5 41.0 46-47 35.5807568121687 38.0 34.0 40.0 28.0 41.0 48-49 35.374129991644246 38.0 34.0 40.0 27.0 41.0 50-51 35.09795251256314 38.0 34.0 40.0 26.5 41.0 52-53 34.8801097004128 38.0 33.0 40.0 26.0 41.0 54-55 34.70072204462785 38.0 33.0 40.0 26.0 41.0 56-57 34.43795394235448 37.0 33.0 40.0 26.0 41.0 58-59 34.18754599271462 37.0 33.0 40.0 25.0 41.0 60-61 33.86912654178073 37.0 32.0 40.0 24.5 41.0 62-63 33.55596255783112 36.0 32.0 39.0 23.5 41.0 64-65 33.249094902728274 36.0 31.5 39.0 23.0 40.5 66-67 32.90610911144371 35.5 31.0 39.0 22.5 40.0 68-69 32.52771598047613 35.0 31.0 38.5 22.0 40.0 70-71 32.12048533545659 35.0 31.0 38.0 21.5 40.0 72-73 31.699604446257553 35.0 30.0 37.0 20.5 39.5 74-75 31.179194338813325 34.0 30.0 37.0 19.0 39.0 76-77 29.818474051910606 32.5 28.5 35.0 18.0 38.0 78-79 30.577799177279697 34.0 29.5 36.0 18.0 38.0 80-81 30.57801151441138 34.0 30.0 35.5 18.0 37.5 82-83 30.29589056324597 34.0 30.0 35.0 18.0 37.0 84-85 29.96828273533513 34.0 29.0 35.0 16.5 36.5 86-87 29.61695053708997 34.0 29.0 35.0 12.0 36.0 88-89 29.262323751982358 34.0 29.0 35.0 7.5 36.0 90-91 28.914828871028885 34.0 29.0 35.0 4.5 35.5 92-93 28.544529474033546 33.0 29.0 35.0 2.0 35.0 94-95 28.10743111094787 33.0 27.5 35.0 2.0 35.0 96-97 27.630053951667804 33.0 27.0 35.0 2.0 35.0 98-99 27.12029874112775 33.0 26.5 35.0 2.0 35.0 100-101 25.877359319681666 32.0 23.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.5391865823491244 1101 2 0.5409628754838423 1101 3 0.5229688938015578 1101 4 0.35281070610749765 1101 5 0.514438054583394 1101 6 0.45248877622812245 1101 7 0.4798786219378499 1101 8 0.3980518039252914 1101 9 0.6495585652445257 1101 10-11 0.6195625017764925 1101 12-13 0.6320371879722941 1101 14-15 0.7049190648627999 1101 16-17 0.6699908943485653 1101 18-19 0.7103308346308452 1101 20-21 0.6968523331537781 1101 22-23 0.6850087373710565 1101 24-25 0.7232640745242875 1101 26-27 0.7992084018926136 1101 28-29 0.7915081062423894 1101 30-31 0.7438372490761722 1101 32-33 0.6345620150931524 1101 34-35 0.6375626393034679 1101 36-37 0.9378244504895648 1101 38-39 0.8909295876646297 1101 40-41 0.8950873400162962 1101 42-43 0.7664347383753736 1101 44-45 0.8008827531624405 1101 46-47 0.8340598925357057 1101 48-49 0.7844250895096181 1101 50-51 0.717586424850218 1101 52-53 0.7519835637819696 1101 54-55 0.7830745458653254 1101 56-57 0.9210182980154684 1101 58-59 0.7330278242423205 1101 60-61 0.7697890232545177 1101 62-63 0.7925560941394707 1101 64-65 0.8595111125380086 1101 66-67 0.7878893263305429 1101 68-69 0.7085317095799084 1101 70-71 0.6802072597574309 1101 72-73 0.7148102010490156 1101 74-75 0.9041865828198397 1101 76-77 0.9157041982698786 1101 78-79 0.8439077368477363 1101 80-81 0.8358396041335965 1101 82-83 0.9450735915761825 1101 84-85 0.9965832843761788 1101 86-87 0.9135813418392438 1101 88-89 0.8447281644885969 1101 90-91 0.7896571192041542 1101 92-93 0.8174502251147544 1101 94-95 0.9831124461131182 1101 96-97 0.9639239933955288 1101 98-99 0.9427373975532163 1101 100-101 1.109976315479269 1102 1 0.4125112351883544 1102 2 0.5193519161392928 1102 3 0.5934093451476699 1102 4 0.30921577127171673 1102 5 0.49941088160734637 1102 6 0.410428836518129 1102 7 0.32228540843400566 1102 8 0.5143952774671732 1102 9 0.7033690273712239 1102 10-11 0.6913752460723757 1102 12-13 0.6100047443591592 1102 14-15 0.7822821798113679 1102 16-17 0.747259527858084 1102 18-19 0.8259052692208044 1102 20-21 0.6749777391956187 1102 22-23 0.7651332222331391 1102 24-25 0.7352463761812018 1102 26-27 0.8140882865830079 1102 28-29 0.9158626353114983 1102 30-31 0.7654114434377988 1102 32-33 0.7524312354265987 1102 34-35 0.7966540829501696 1102 36-37 0.8732235800500519 1102 38-39 0.8550122545279777 1102 40-41 0.8882513938192247 1102 42-43 0.8864941583518231 1102 44-45 0.9932431952282457 1102 46-47 1.014385968248284 1102 48-49 0.9577849431073417 1102 50-51 0.8830557937964088 1102 52-53 0.985043013216071 1102 54-55 0.855448527035712 1102 56-57 0.7537824972341696 1102 58-59 0.8463441238947382 1102 60-61 0.788228945732925 1102 62-63 0.7884357899811789 1102 64-65 0.7871854282240704 1102 66-67 0.906080468618562 1102 68-69 0.8167719296948306 1102 70-71 0.8243641850356838 1102 72-73 0.8087357124533519 1102 74-75 0.8982126156247077 1102 76-77 1.0477458628147573 1102 78-79 0.9643601539398468 1102 80-81 0.9145892841526511 1102 82-83 1.110580239301651 1102 84-85 1.2196041808160736 1102 86-87 1.1403496404677291 1102 88-89 1.138940739619894 1102 90-91 1.0441892025889636 1102 92-93 1.1570663553057265 1102 94-95 1.1251401993365064 1102 96-97 1.2820757674658019 1102 98-99 1.3106953333889564 1102 100-101 1.3867304904171043 1103 1 0.37398973648876677 1103 2 0.5059141484108096 1103 3 0.4315101374324932 1103 4 0.2185053409257094 1103 5 0.2832217267512789 1103 6 0.3723846125600758 1103 7 0.4353384677738532 1103 8 0.3205588179222829 1103 9 0.4089796862882906 1103 10-11 0.40264465245508063 1103 12-13 0.4253541276330992 1103 14-15 0.585367451366686 1103 16-17 0.5415188274393472 1103 18-19 0.5948802226914367 1103 20-21 0.5931720606984712 1103 22-23 0.5579860822161393 1103 24-25 0.6198525151029415 1103 26-27 0.6244812668585027 1103 28-29 0.6548123354556026 1103 30-31 0.8169640634704294 1103 32-33 0.6694179818068307 1103 34-35 0.6707990204131704 1103 36-37 0.6044596362857533 1103 38-39 0.5711062717435738 1103 40-41 0.6105235585917015 1103 42-43 0.5461682175614655 1103 44-45 0.5651414838448829 1103 46-47 0.7029555007881996 1103 48-49 0.7586532176858398 1103 50-51 0.6807701926786152 1103 52-53 0.5611152674912461 1103 54-55 0.6497943203545233 1103 56-57 0.7388107102768373 1103 58-59 0.6667584513279792 1103 60-61 0.5634339101780341 1103 62-63 0.4913502623912578 1103 64-65 0.5136513083320509 1103 66-67 0.5254644209392296 1103 68-69 0.7015013262956984 1103 70-71 0.6454595754282622 1103 72-73 0.5164563590172939 1103 74-75 0.6616699141971552 1103 76-77 0.7804344777004317 1103 78-79 0.8693532114633804 1103 80-81 0.7633318151093107 1103 82-83 0.8477939373712147 1103 84-85 0.9119155385762738 1103 86-87 0.9026127127296633 1103 88-89 0.9251466570826565 1103 90-91 0.8534819453212457 1103 92-93 0.8420129436447716 1103 94-95 0.9293155874094552 1103 96-97 0.7815195487247273 1103 98-99 0.8351392514184965 1103 100-101 1.086121861175986 1104 1 0.4339087673912019 1104 2 0.5268064118325242 1104 3 0.43103124256152014 1104 4 0.210960320588498 1104 5 0.4009347189370658 1104 6 0.38049565709940936 1104 7 0.40294829714382274 1104 8 0.5645281180184796 1104 9 0.5549831292681517 1104 10-11 0.5733960954687021 1104 12-13 0.5926799955509381 1104 14-15 0.6340387818083784 1104 16-17 0.6523020842620255 1104 18-19 0.71141802374121 1104 20-21 0.658453764804733 1104 22-23 0.6814184926395868 1104 24-25 0.6000498957378682 1104 26-27 0.7656075769629709 1104 28-29 0.6900018259283343 1104 30-31 0.8106632204466777 1104 32-33 0.8894603650577508 1104 34-35 0.7337166293986783 1104 36-37 0.6962649433691723 1104 38-39 0.5713451917618002 1104 40-41 0.702552279625948 1104 42-43 0.5543591130737795 1104 44-45 0.7620404813301604 1104 46-47 0.8743706456802656 1104 48-49 0.8944035636715668 1104 50-51 0.8916883628360779 1104 52-53 0.8771910510223293 1104 54-55 0.8245509700313249 1104 56-57 0.8588495070903974 1104 58-59 0.8758481713326987 1104 60-61 0.6879817026203554 1104 62-63 0.6541182958421743 1104 64-65 0.6253023775123197 1104 66-67 0.6243376467075734 1104 68-69 0.5125078915857344 1104 70-71 0.5649283396685334 1104 72-73 0.6751957684787406 1104 74-75 0.7097423095997613 1104 76-77 0.8335044680803421 1104 78-79 0.7193812700615432 1104 80-81 0.6823183090081493 1104 82-83 0.854757532922342 1104 84-85 0.9493242953905039 1104 86-87 0.9794402135313369 1104 88-89 1.0502115294054875 1104 90-91 0.8955447375104093 1104 92-93 0.8507305180316997 1104 94-95 0.9418164312770649 1104 96-97 0.8668766524023042 1104 98-99 1.1035624636849555 1104 100-101 1.3220876043701928 1105 1 0.1576491117656218 1105 2 0.33540688277470565 1105 3 0.34766636560622644 1105 4 0.1335817676296216 1105 5 0.29453296134283846 1105 6 0.22065777492998961 1105 7 0.3116839960030049 1105 8 0.2924946932496084 1105 9 0.36264814747332963 1105 10-11 0.3156006218555589 1105 12-13 0.3722339113843631 1105 14-15 0.48612172596714487 1105 16-17 0.4524415930991452 1105 18-19 0.48928466304275986 1105 20-21 0.43482064826211086 1105 22-23 0.5288844951696277 1105 24-25 0.47771594627976555 1105 26-27 0.5398375848611678 1105 28-29 0.596308461067764 1105 30-31 0.5367784513459881 1105 32-33 0.5592233249801382 1105 34-35 0.5699774771273738 1105 36-37 0.5558357084126158 1105 38-39 0.6603232972108941 1105 40-41 0.6429220323239448 1105 42-43 0.6002127858984139 1105 44-45 0.6176085860582532 1105 46-47 0.6302581398221676 1105 48-49 0.7647522108188767 1105 50-51 0.6991307322291007 1105 52-53 0.5879594905224792 1105 54-55 0.7070658283062343 1105 56-57 0.5709061143731162 1105 58-59 0.5105906442871202 1105 60-61 0.5533757480038446 1105 62-63 0.5282247305771861 1105 64-65 0.5605910467155795 1105 66-67 0.5807474335873906 1105 68-69 0.6020516315286457 1105 70-71 0.5735535744022329 1105 72-73 0.6436127169897183 1105 74-75 0.6343520289680384 1105 76-77 0.7109456166942252 1105 78-79 0.837597138223952 1105 80-81 0.8084050277504673 1105 82-83 0.8655978877839949 1105 84-85 0.8113054847407213 1105 86-87 0.7608491606134287 1105 88-89 0.8006022401775112 1105 90-91 0.634630849140418 1105 92-93 0.7565998438666668 1105 94-95 0.6900760016939032 1105 96-97 0.6572945808138506 1105 98-99 0.7301944364640391 1105 100-101 1.082641711103978 1106 1 0.114574403876599 1106 2 0.1456535620915922 1106 3 0.13310898840314422 1106 4 0.041101373712528755 1106 5 0.1000915484140279 1106 6 0.10442770518948663 1106 7 0.15980388912154098 1106 8 0.032608920758796955 1106 9 0.15787708949001455 1106 10-11 0.1685347573866025 1106 12-13 0.12304831339150013 1106 14-15 0.21334311305570708 1106 16-17 0.16326668559948843 1106 18-19 0.10031675916566485 1106 20-21 0.10156799236104064 1106 22-23 0.19098481917020393 1106 24-25 0.01780001509790452 1106 26-27 0.20922117407398133 1106 28-29 0.2391262920124646 1106 30-31 0.23926619131001559 1106 32-33 0.30470802912573447 1106 34-35 0.20507873170545565 1106 36-37 0.20394333856144442 1106 38-39 0.2172272904797552 1106 40-41 0.24555223396472314 1106 42-43 0.3099477062595142 1106 44-45 0.17089206953962588 1106 46-47 -0.029898298119583444 1106 48-49 0.25944891648061486 1106 50-51 0.09490663789927822 1106 52-53 0.009027917893433823 1106 54-55 0.1917506113103471 1106 56-57 0.392120817263077 1106 58-59 0.5170595074922204 1106 60-61 0.16767337321228837 1106 62-63 0.10501996479990794 1106 64-65 0.0025311658717441787 1106 66-67 0.014823410019019434 1106 68-69 0.2472938926559749 1106 70-71 0.19969934062661565 1106 72-73 0.25093922530565393 1106 74-75 0.3821124234484117 1106 76-77 0.3060364389468795 1106 78-79 0.4592214477718173 1106 80-81 0.4461835995897623 1106 82-83 0.5198132396138746 1106 84-85 0.41390810936828615 1106 86-87 0.36248189581894863 1106 88-89 0.526875740780099 1106 90-91 0.49388158755895617 1106 92-93 0.5719459236475508 1106 94-95 0.5798829508665335 1106 96-97 0.4517519532056191 1106 98-99 0.43431303404970123 1106 100-101 0.676148028586983 1107 1 0.18725252283672944 1107 2 0.22109324120297913 1107 3 0.2651883663942769 1107 4 0.06968068291509866 1107 5 0.22713667237368185 1107 6 0.257588439343742 1107 7 0.3362338563568201 1107 8 0.24483632104544029 1107 9 0.34623886655898417 1107 10-11 0.18486135478385535 1107 12-13 0.17826115053892266 1107 14-15 0.2634211847320529 1107 16-17 0.2659673378218983 1107 18-19 0.3245941787531734 1107 20-21 0.3506092935467393 1107 22-23 0.34200743123878397 1107 24-25 0.3621605553084919 1107 26-27 0.34488590531106667 1107 28-29 0.4229607132736035 1107 30-31 0.36823171822370426 1107 32-33 0.4760095754658451 1107 34-35 0.24924733179814496 1107 36-37 0.38391057383569205 1107 38-39 0.3261229202022946 1107 40-41 0.3050636882617397 1107 42-43 0.3668371810755602 1107 44-45 0.4753478986020596 1107 46-47 0.2506675311966333 1107 48-49 0.3829581081014979 1107 50-51 0.3111297853165027 1107 52-53 0.2573970279564648 1107 54-55 0.3264098942263445 1107 56-57 0.3550427816506385 1107 58-59 0.34019443591872545 1107 60-61 0.2228705158824269 1107 62-63 0.02790164446962251 1107 64-65 0.14027758586129835 1107 66-67 0.20482108590068293 1107 68-69 0.32790941001940155 1107 70-71 0.18858998818742378 1107 72-73 0.20344473172970368 1107 74-75 0.299050905104572 1107 76-77 0.33083905585070283 1107 78-79 0.42016256877156266 1107 80-81 0.42181308169872267 1107 82-83 0.37541937368344946 1107 84-85 0.3844312096352951 1107 86-87 0.44436265519122387 1107 88-89 0.49145430733336326 1107 90-91 0.43106728060703503 1107 92-93 0.3112319384658093 1107 94-95 0.47003596868076514 1107 96-97 0.6883495374582473 1107 98-99 0.45206716102937605 1107 100-101 0.7776649165123821 1108 1 0.301718203606697 1108 2 0.35785926221550923 1108 3 0.39747367310771153 1108 4 0.21366495811891184 1108 5 0.17446225648096458 1108 6 0.20894338777208077 1108 7 0.2939489969788127 1108 8 0.3185898896925039 1108 9 0.4078380066044147 1108 10-11 0.42937795914854604 1108 12-13 0.45502437857255273 1108 14-15 0.4613817844215191 1108 16-17 0.6032287637054949 1108 18-19 0.6240909359536531 1108 20-21 0.5662583294624213 1108 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-1.0262476111489285 2316 84-85 -1.0010598791351626 2316 86-87 -1.0385456309536956 2316 88-89 -1.1320095072367025 2316 90-91 -0.9113676082606439 2316 92-93 -1.054045453936837 2316 94-95 -0.9148042307425399 2316 96-97 -0.9348871955866649 2316 98-99 -0.9261787951214728 2316 100-101 -1.1580436533179572 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 25.0 4 67.0 5 116.0 6 224.0 7 561.0 8 1456.0 9 2434.0 10 3831.0 11 6080.0 12 8405.0 13 10775.0 14 12833.0 15 15675.0 16 18387.0 17 20970.0 18 19286.0 19 17225.0 20 17714.0 21 18917.0 22 21529.0 23 24750.0 24 29960.0 25 37705.0 26 48325.0 27 61103.0 28 77062.0 29 94946.0 30 114738.0 31 136627.0 32 163987.0 33 196362.0 34 240156.0 35 297967.0 36 383910.0 37 482320.0 38 396263.0 39 66693.0 40 9.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.588318836482145 21.096042636781473 14.642043831340814 23.67359469539557 2 15.686527218771715 21.565199482768403 39.48544441678792 23.262828881671958 3 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21.594850258870938 22-23 22.227035563945563 26.824407271673113 28.84661200772809 22.10194515665323 24-25 22.187136708341875 27.041098585775774 28.80239393318303 21.969370772699325 26-27 22.111778836299838 27.19828158690858 28.686135456207523 22.003804120584064 28-29 22.0768635316259 27.286853787058828 28.65848809508389 21.977794586231383 30-31 22.3014766421743 27.098468884190723 28.608611852065874 21.9914426215691 32-33 22.194436171315438 27.19866421403015 28.46953709984479 22.13736251480963 34-35 22.332987753621452 27.246527776638963 28.54930644086897 21.87117802887061 36-37 22.404100634833654 27.054929007734845 28.50678080128708 22.03418955614442 38-39 22.251069644717596 27.15067700713949 28.551143397487422 22.047109950655493 40-41 22.342631107403196 27.05961502388064 28.591786660160324 22.00596720855584 42-43 22.274932964616863 27.178354786405066 28.36469085012935 22.182021398848722 44-45 22.311893989954562 27.250360346916924 28.31433073861689 22.123414924511625 46-47 22.427871351137735 27.272840567993896 28.05679610973869 22.24249197112968 48-49 22.431439093987052 27.325267444066608 27.97468954317764 22.268603918768697 50-51 22.436765173598424 27.420935110541024 27.815816000651868 22.32648371520869 52-53 22.513694012037934 27.389812212811265 27.814798227611785 22.281695547539016 54-55 22.363749730225088 27.359341493382068 27.917111371611085 22.359797404781755 56-57 22.4197101023392 27.473405057794558 27.73514008834152 22.371744751524723 58-59 22.34375927627278 27.387624464005683 27.901060525004652 22.367555734716884 60-61 22.421023084556644 27.455288262070653 27.898480644969187 22.22520800840351 62-63 22.34757992989947 27.470062189765116 27.810257864645187 22.37210001569023 64-65 22.18840400117846 27.362033776458894 27.870001912122778 22.57956031023987 66-67 22.230576860704375 27.435024220244685 27.745787226890318 22.588611692160622 68-69 22.249680385772606 27.443770628843556 27.821513693637474 22.48503529174636 70-71 22.207549907206634 27.258962092908906 27.83917501544368 22.69431298444078 72-73 22.286213289616892 27.354499446789664 27.70751048457878 22.65177677901466 74-75 22.206459845880445 27.518840888426045 27.625167074614716 22.64953219107879 76-77 22.130406590860392 27.44538052536192 27.624001669454845 22.800211214322843 78-79 22.06349497865988 27.46778976421904 27.674631852566556 22.794083404554527 80-81 22.131207080280994 27.54165162941007 27.561114662845988 22.766026627462946 82-83 22.25376763136044 27.373371644745387 27.660395698033312 22.71246502586086 84-85 22.17944507362197 27.426124292503218 27.513535059653627 22.880895574221185 86-87 22.057679619177044 27.495281032702866 27.567869178027387 22.8791701700927 88-89 21.999392665617496 27.35278945861649 27.560371890649645 23.08744598511637 90-91 22.05501443001443 27.316345270890725 27.620539813721635 23.008100485373213 92-93 22.063341185546417 27.228778852947137 27.643824401686945 23.064055559819497 94-95 21.861081031801575 27.19404423112366 27.681115885176688 23.26375885189808 96-97 21.83537330015518 27.05745662419705 27.72048956580254 23.386680509845228 98-99 21.70908599604643 27.09910421604803 27.619633527426412 23.572176260479125 100-101 21.739009954758252 27.063129878835024 27.603679548343347 23.594180618063383 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 398.0 1 294.0 2 239.0 3 323.5 4 308.5 5 288.0 6 442.5 7 883.0 8 1402.0 9 1642.5 10 1483.0 11 1270.5 12 1318.0 13 1477.5 14 1691.5 15 1938.5 16 2163.0 17 2384.5 18 2720.0 19 3161.0 20 3737.0 21 4467.0 22 5423.5 23 6797.0 24 8754.0 25 11661.5 26 15774.5 27 21064.5 28 27401.5 29 35281.5 30 43905.0 31 52037.0 32 60890.0 33 67296.0 34 71266.5 35 74803.5 36 80223.0 37 86722.5 38 90185.0 39 92150.0 40 93957.0 41 96440.5 42 98807.0 43 101376.0 44 104368.0 45 107771.0 46 110765.0 47 113010.5 48 114426.0 49 113222.5 50 109785.0 51 106513.5 52 104885.0 53 103002.5 54 98873.0 55 91842.0 56 82997.5 57 73458.0 58 64639.5 59 56638.5 60 47816.5 61 39273.5 62 32119.5 63 25234.0 64 19037.0 65 14076.0 66 10206.5 67 7865.0 68 5944.5 69 4320.0 70 3229.5 71 2344.0 72 1661.5 73 1246.5 74 922.5 75 677.5 76 460.5 77 283.0 78 176.5 79 109.5 80 60.0 81 28.0 82 20.5 83 13.0 84 7.5 85 6.5 86 5.5 87 3.0 88 0.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.018790606402054703 2 0.0027874372498684985 3 0.003836825390995463 4 0.03318689996314024 5 0.001770842488151752 6 6.558675882043526E-5 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 4.9190069115326447E-5 24-25 6.886609676145703E-4 26-27 0.002328329938125452 28-29 1.4757020734597933E-4 30-31 0.0070341798834916815 32-33 0.023086539104793212 34-35 0.013543665696419882 36-37 4.755040014481557E-4 38-39 6.230742087941349E-4 40-41 0.0020987762822539282 42-43 0.008526278646656584 44-45 0.008231138231964625 46-47 0.007985187886387992 48-49 0.009854410512770398 50-51 0.1908574681674666 52-53 0.050206663877043194 54-55 0.018298705710901437 56-57 0.010493881411269642 58-59 0.020430275372565584 60-61 0.05320725809307811 62-63 0.09555990760137417 64-65 0.09980665023499737 66-67 0.10441412004213295 68-69 0.10315157493483956 70-71 0.0931987842838385 72-73 0.07157155056279997 74-75 0.24076899162981785 76-77 0.29128719261125813 78-79 0.006886609676145703 80-81 8.362311749605496E-4 82-83 0.0 84-85 1.6396689705108814E-5 86-87 0.0 88-89 1.6396689705108815E-4 90-91 0.006427502364402656 92-93 0.018823399781464918 94-95 0.0016396689705108815 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3049396.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 61.234254999284566 #Duplication Level Percentage of deduplicated Percentage of total 1 78.24408992805434 47.91218554841433 2 12.559267183921511 15.381147386887928 3 3.9365665943548658 7.231581679811732 4 1.7385289007105231 4.258300881189363 5 0.9670882952151367 2.9609465638013535 6 0.570735476604015 2.096913702690508 7 0.3811859001677847 1.6339144229104172 8 0.27127432535906265 1.3289024971036583 9 0.19884543628903487 1.0958536936050078 >10 1.0651134164906646 11.394716304582275 >50 0.04892246947499851 2.022065851022183 >100 0.017523134587143042 1.9538597315072008 >500 6.442040782884337E-4 0.26166746674782676 >1k 1.610510194580499E-4 0.29128585697539877 >5k 5.3683673152683295E-5 0.17665841275090768 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5374 0.17623162095050954 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4665 0.15298111494866526 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3057 0.10024936085703529 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 3.279337941021763E-5 2 0.0 0.0 0.0 0.0 3.279337941021763E-5 3 0.0 0.0 0.0 0.0 3.279337941021763E-5 4 0.0 0.0 0.0 0.0 3.279337941021763E-5 5 0.0 0.0 0.0 0.0 3.279337941021763E-5 6 0.0 0.0 0.0 0.0 9.838013823065289E-5 7 0.0 0.0 0.0 0.0 1.3117351764087052E-4 8 0.0 0.0 0.0 0.0 1.3117351764087052E-4 9 0.0 0.0 0.0 3.279337941021763E-5 1.3117351764087052E-4 10-11 0.0 0.0 0.0 3.279337941021763E-5 2.2955365587152342E-4 12-13 0.0 0.0 0.0 3.279337941021763E-5 3.6072717351239394E-4 14-15 0.0 0.0 0.0 4.919006911532644E-5 5.574874499736998E-4 16-17 0.0 0.0 0.0 9.838013823065289E-5 7.378510367298967E-4 18-19 0.0 0.0 0.0 1.3117351764087052E-4 8.362311749605495E-4 20-21 0.0 0.0 0.0 1.8036358675619697E-4 9.346113131912025E-4 22-23 0.0 0.0 0.0 2.131569661664146E-4 0.001065784830832073 24-25 0.0 0.0 0.0 2.9514041469195867E-4 0.001147768279357617 26-27 0.0 0.0 0.0 4.755040014481557E-4 0.0012133550381780525 28-29 0.0 0.0 0.0 0.0010985782102422906 0.001328131866113814 30-31 0.0 0.0 0.0 0.004623866496840686 0.0013773219352291406 32-33 0.0 0.0 0.0 0.015019367769879674 0.0014429086940495757 34-35 0.0 0.0 0.0 0.03148164423380892 0.0014757020734597933 36-37 0.0 0.0 0.0 0.0574703974164064 0.0014757020734597933 38-39 0.0 0.0 0.0 0.09365789159558155 0.0014757020734597933 40-41 0.0 0.0 0.0 0.1321245256437668 0.0014920987631649022 42-43 0.0 0.0 0.0 0.17287029956096223 0.0015248921425751198 44-45 0.0 0.0 0.0 0.22109296398368727 0.0016068755911006639 46-47 0.0 0.0 0.0 0.2817607158925899 0.001672462349921099 48-49 0.0 0.0 0.0 0.3465440369174748 0.001852825936677296 50-51 0.0 0.0 0.0 0.4092449783498109 0.0019839994543181666 52-53 0.0 0.0 0.0 0.47599590213930887 0.0022955365587152337 54-55 0.0 0.0 0.0 0.5539949550665115 0.0023775200072407784 56-57 3.279337941021763E-5 0.0 0.0 0.6426354596123298 0.0024595034557663223 58-59 3.279337941021763E-5 0.0 0.0 0.738506904318101 0.002541486904291866 60-61 3.279337941021763E-5 0.0 0.0 0.8468726265791652 0.0025578835939969752 62-63 3.279337941021763E-5 0.0 0.0 0.9556482660828571 0.0027218504910480634 64-65 3.279337941021763E-5 0.0 0.0 1.0717532258847324 0.0027874372498684985 66-67 3.279337941021763E-5 0.0 0.0 1.2078949405062511 0.0028694206983940424 68-69 3.279337941021763E-5 0.0 0.0 1.3637946662224256 0.002918610767509369 70-71 3.279337941021763E-5 0.0 0.0 1.5320574959762525 0.0029678008366246953 72-73 3.279337941021763E-5 0.0 0.0 1.7103550998296055 0.003230147871906437 74-75 3.279337941021763E-5 0.0 0.0 1.8916532978989937 0.0033941147689575245 76-77 3.279337941021763E-5 0.0 0.0 2.1004323479141442 0.00345970152777796 78-79 3.279337941021763E-5 0.0 0.0 2.3338392258663685 0.003476098217483069 80-81 3.279337941021763E-5 0.0 0.0 2.5949565094202263 0.003541684976303504 82-83 3.279337941021763E-5 0.0 0.0 2.873355903923269 0.003722048563059701 84-85 3.279337941021763E-5 0.0 0.0 3.1594289492083023 0.00373844525276481 86-87 3.279337941021763E-5 0.0 0.0 3.4603573953661644 0.003902412149815898 88-89 3.279337941021763E-5 3.279337941021763E-5 0.0 3.799244178191353 0.004066379046866986 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3375 0.0 57.828533 1 GTATCAA 6590 0.0 42.37074 1 ATCAACG 8245 0.0 33.45491 3 CAACGCA 8315 0.0 33.21783 5 TCAACGC 8350 0.0 33.03313 4 TATCAAC 8700 0.0 31.870533 2 AACGCAG 8805 0.0 31.584848 6 ACGCAGA 10200 0.0 26.846409 7 CGCAGAG 10515 0.0 25.997032 8 GCAGAGT 12040 0.0 22.349464 9 GTACATG 8260 0.0 21.616327 1 TACATGG 8590 0.0 20.339973 2 ACATGGG 8450 0.0 19.889366 3 AGAGTAC 10995 0.0 19.682495 10-11 CAGAGTA 11725 0.0 18.942774 10-11 GAGTACT 7725 0.0 18.737513 12-13 TACCCCG 260 3.3150536E-7 18.253124 5 TATACTG 1595 0.0 17.852585 5 GAGTACA 6750 0.0 17.517403 1 GTACTTT 8255 0.0 17.477007 14-15 >>END_MODULE