FastQCFastQC Report
Sat 18 Jun 2016
SRR1930909_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1930909_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2458485
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN44260.1800295710569721No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA18550.054.633551
GTATCAA36450.046.478171
TCAACGC44550.038.2299044
ATCAACG44200.037.4038543
TATCAAC45050.036.253562
CAACGCA46650.035.88815
AACGCAG50900.033.365556
ACGCAGA58000.028.5178327
GTACATG40950.028.072961
CGCAGAG59300.027.659558
TACATGG43350.026.3959432
GCAGAGT59600.024.8407129
ACATGGG45250.024.728583
AGAGTAC53450.020.74161710-11
CATGGGG34550.020.7097324
GAGTACT38500.019.90285512-13
TAACGCA3803.6379788E-1219.8903244
CAGAGTA58050.019.70792410-11
GTATTAG7350.019.2078151
AGTACTT43750.017.93402312-13
Produced by FastQC (version 0.11.5)