##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1930909_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2458485 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 21.39509657370291 28.0 10.0 31.0 2.0 31.0 2 20.155074771658157 27.0 2.0 31.0 2.0 31.0 3 20.492251935643292 26.0 10.0 31.0 2.0 31.0 4 22.31327463864941 30.0 2.0 35.0 2.0 35.0 5 22.337259735162103 30.0 2.0 35.0 2.0 35.0 6 22.969654889088197 30.0 10.0 35.0 2.0 37.0 7 22.91755776423285 30.0 10.0 35.0 2.0 36.0 8 23.40087289529934 30.0 10.0 35.0 2.0 36.0 9 24.29848300884488 30.0 10.0 35.0 2.0 38.0 10-11 24.55125961720328 32.0 10.0 37.0 2.0 39.0 12-13 24.379953304575785 31.5 10.0 37.0 2.0 39.0 14-15 24.384930556826664 31.0 10.0 37.0 2.0 39.0 16-17 23.594612739146264 30.5 9.0 36.5 2.0 39.0 18-19 23.076605307740337 29.5 2.0 36.0 2.0 39.0 20-21 22.509621982643782 27.0 2.0 36.0 2.0 39.0 22-23 22.359371320142284 27.0 2.0 36.0 2.0 39.0 24-25 22.04224268197691 27.0 2.0 36.0 2.0 39.0 26-27 21.62651348289699 27.0 2.0 36.0 2.0 38.5 28-29 21.419757492927555 27.0 2.0 35.5 2.0 38.0 30-31 21.203268069563165 26.5 2.0 35.0 2.0 38.0 32-33 20.777881093437625 25.5 2.0 35.0 2.0 38.0 34-35 18.23063675393586 16.0 2.0 34.0 2.0 38.0 36-37 16.69557227316823 14.0 2.0 31.5 2.0 37.5 38-39 15.857883615315936 10.0 2.0 30.0 2.0 37.0 40-41 16.806062066679274 12.0 2.0 31.5 2.0 37.0 42-43 16.638385428424414 12.0 2.0 32.0 2.0 37.0 44-45 14.989949908175156 8.0 2.0 30.5 2.0 36.5 46-47 15.401793991014792 11.5 2.0 30.0 2.0 36.0 48-49 14.901089288728627 7.5 2.0 30.0 2.0 36.0 50-51 13.387222618807925 6.5 2.0 27.0 2.0 34.5 52-53 15.549805469628652 11.5 2.0 30.5 2.0 36.0 54-55 16.819497576759673 12.0 2.0 33.0 2.0 37.0 56-57 16.92387242549782 8.5 2.0 33.0 2.0 38.0 58-59 16.550012304325634 8.0 2.0 33.0 2.0 37.5 60-61 16.17925572049453 7.0 2.0 32.0 2.0 37.0 62-63 15.679428387807938 2.0 2.0 31.5 2.0 37.0 64-65 15.162383541083228 2.0 2.0 31.0 2.0 36.0 66-67 14.70985057871006 2.0 2.0 31.0 2.0 36.0 68-69 14.219469714071877 2.0 2.0 30.0 2.0 35.0 70-71 13.653649096903173 2.0 2.0 29.5 2.0 34.5 72-73 13.14864947315115 2.0 2.0 29.0 2.0 34.0 74-75 12.60869620925082 2.0 2.0 28.0 2.0 34.0 76-77 12.051616951089798 2.0 2.0 27.0 2.0 34.0 78-79 11.539738294112023 2.0 2.0 26.0 2.0 33.0 80-81 11.027127885669426 2.0 2.0 26.0 2.0 32.5 82-83 10.53244294758764 2.0 2.0 25.0 2.0 32.0 84-85 10.023736569472664 2.0 2.0 22.5 2.0 32.0 86-87 9.486429447403584 2.0 2.0 19.5 2.0 31.0 88-89 8.97046799146629 2.0 2.0 15.5 2.0 31.0 90-91 8.445051932389255 2.0 2.0 7.0 2.0 31.0 92-93 7.890621459964166 2.0 2.0 2.0 2.0 30.5 94-95 7.327205169037029 2.0 2.0 2.0 2.0 30.0 96-97 6.739300422821372 2.0 2.0 2.0 2.0 29.0 98-99 6.1306373640677085 2.0 2.0 2.0 2.0 29.0 100-101 5.447799356107522 2.0 2.0 2.0 2.0 25.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 6.770827965183756 1101 2 7.472406346201453 1101 3 6.934223629038726 1101 4 8.790386517311347 1101 5 8.454350798539174 1101 6 7.947836132768323 1101 7 7.575967114808019 1101 8 6.907383949046963 1101 9 7.162407319944851 1101 10-11 6.731574552660955 1101 12-13 6.4001055449423525 1101 14-15 6.916994437972736 1101 16-17 7.576748664912895 1101 18-19 7.837692484250358 1101 20-21 8.264436730219881 1101 22-23 8.239086948292229 1101 24-25 7.964037473159404 1101 26-27 7.969338269026871 1101 28-29 7.661559446736245 1101 30-31 7.634542017557369 1101 32-33 7.873002310615341 1101 34-35 10.057590233641484 1101 36-37 4.938312774324974 1101 38-39 6.8459593017829174 1101 40-41 8.429985109171835 1101 42-43 8.886660203678865 1101 44-45 3.9517471007893636 1101 46-47 4.934563734343143 1101 48-49 7.251484713288505 1101 50-51 -2.5771139384349606 1101 52-53 4.884651571188284 1101 54-55 7.36378511667467 1101 56-57 8.10075702470802 1101 58-59 8.25976240955432 1101 60-61 7.809103360695747 1101 62-63 8.0262146056403 1101 64-65 7.797637643903615 1101 66-67 7.787514479898464 1101 68-69 7.647726708408763 1101 70-71 7.540750039237638 1101 72-73 7.360192273788776 1101 74-75 7.362477270768844 1101 76-77 7.07427199294794 1101 78-79 6.889157825035161 1101 80-81 6.774298741686044 1101 82-83 6.425266028449897 1101 84-85 6.28747873179687 1101 86-87 6.068116555922382 1101 88-89 5.744063477136402 1101 90-91 5.641060694871356 1101 92-93 5.389133619761132 1101 94-95 4.969726423557298 1101 96-97 4.689697514683361 1101 98-99 4.205451029390239 1101 100-101 3.647659468519959 1102 1 6.528369767054109 1102 2 7.213624654638409 1102 3 7.0975119390052015 1102 4 8.76969602212898 1102 5 8.752963837103678 1102 6 8.084737630051066 1102 7 7.7956067925382015 1102 8 7.09323562638934 1102 9 6.9996270409985435 1102 10-11 6.756149043868675 1102 12-13 6.51731493387361 1102 14-15 6.49934568883506 1102 16-17 6.993822444737187 1102 18-19 7.429722137301749 1102 20-21 7.727584356019246 1102 22-23 7.502319292595512 1102 24-25 7.182733452206758 1102 26-27 7.298140127828894 1102 28-29 7.315071440853373 1102 30-31 7.36317041430387 1102 32-33 7.155301568361541 1102 34-35 9.0585917705097 1102 36-37 1.2529792781230462 1102 38-39 2.4235182083559597 1102 40-41 6.7099422266636815 1102 42-43 7.955594833054221 1102 44-45 1.4243376634875329 1102 46-47 1.4178799116398437 1102 48-49 5.854675473599016 1102 50-51 -3.420755010581667 1102 52-53 3.7927428445698705 1102 54-55 6.613135508976818 1102 56-57 7.095418148942343 1102 58-59 7.42559087462163 1102 60-61 7.2397416387593445 1102 62-63 7.154520351063384 1102 64-65 6.845846952683923 1102 66-67 6.732583603967582 1102 68-69 6.764835996153826 1102 70-71 6.5732759246693835 1102 72-73 6.352161428623262 1102 74-75 6.161822780513136 1102 76-77 6.161676166187396 1102 78-79 5.980860136707554 1102 80-81 5.970708644143388 1102 82-83 5.680048310119933 1102 84-85 5.498890862030615 1102 86-87 5.309516429010879 1102 88-89 4.930105031728823 1102 90-91 4.765382780588073 1102 92-93 4.459040385656309 1102 94-95 4.033900034014581 1102 96-97 3.9689945243529268 1102 98-99 3.530930823825803 1102 100-101 3.0896219557754323 1103 1 6.3308875774445745 1103 2 7.257408655614345 1103 3 6.958977940375263 1103 4 8.595626722200382 1103 5 8.576501960424693 1103 6 7.857323235519033 1103 7 7.6481472524732474 1103 8 6.972308897452777 1103 9 7.064895777205141 1103 10-11 6.538826109136046 1103 12-13 6.40877336833368 1103 14-15 6.774052332445539 1103 16-17 7.236361531197026 1103 18-19 7.81079854944327 1103 20-21 7.954376080020683 1103 22-23 7.863791265596912 1103 24-25 7.860888627606368 1103 26-27 7.752463034409548 1103 28-29 7.627842883488601 1103 30-31 7.261370735236401 1103 32-33 7.292001167153227 1103 34-35 8.860832753133103 1103 36-37 -2.5999233185981883 1103 38-39 -1.7409208976396435 1103 40-41 5.01725116218627 1103 42-43 7.070637116469555 1103 44-45 0.8339704166911002 1103 46-47 -0.07298571044631785 1103 48-49 5.42831490138024 1103 50-51 -3.928184658366318 1103 52-53 3.0346212979856038 1103 54-55 5.885169847563194 1103 56-57 6.36920464947254 1103 58-59 6.711695656277463 1103 60-61 6.776147433289157 1103 62-63 6.725153659628662 1103 64-65 6.7067401039905565 1103 66-67 6.519922721821141 1103 68-69 6.572570282445772 1103 70-71 6.616279604047373 1103 72-73 6.32519575953183 1103 74-75 6.204926442061543 1103 76-77 5.974182297773146 1103 78-79 5.549326766805089 1103 80-81 5.358061752676077 1103 82-83 5.165841202001841 1103 84-85 5.136685487391793 1103 86-87 4.797044676959837 1103 88-89 4.556482409824367 1103 90-91 4.129130371674645 1103 92-93 4.087480962379402 1103 94-95 3.825608679827744 1103 96-97 3.492290284750603 1103 98-99 3.1636580081419092 1103 100-101 2.916423405005049 1104 1 6.130956159790337 1104 2 6.91868453218245 1104 3 6.658664012852107 1104 4 8.187180479737297 1104 5 8.302780021013927 1104 6 7.670573508011387 1104 7 7.319564335192535 1104 8 6.5064518834791265 1104 9 6.683528947383252 1104 10-11 6.1071063659932605 1104 12-13 5.9221110016712295 1104 14-15 6.3855762076746565 1104 16-17 6.884037283663748 1104 18-19 7.277371595764929 1104 20-21 7.341752029661645 1104 22-23 7.109020506169902 1104 24-25 7.065767363405126 1104 26-27 7.235343166348919 1104 28-29 6.911899529240376 1104 30-31 6.5518202353609105 1104 32-33 6.6453223080695345 1104 34-35 8.450914031377398 1104 36-37 -2.7964999165056117 1104 38-39 -2.021755806053637 1104 40-41 4.553454067954942 1104 42-43 6.641675771602408 1104 44-45 0.3877744916254766 1104 46-47 -0.33156401474781916 1104 48-49 5.021441970587176 1104 50-51 -4.018764791530373 1104 52-53 2.7674261118131103 1104 54-55 5.275271128991669 1104 56-57 5.85671286506604 1104 58-59 6.155750424179292 1104 60-61 6.035225817195169 1104 62-63 6.151412533490218 1104 64-65 6.157600445024437 1104 66-67 6.02182588191601 1104 68-69 5.963557419173659 1104 70-71 5.74673669721731 1104 72-73 5.557878104884761 1104 74-75 5.475134352708546 1104 76-77 5.519796905812791 1104 78-79 5.363753553109847 1104 80-81 5.2368563363036795 1104 82-83 4.784249030186736 1104 84-85 4.569797288862231 1104 86-87 4.339680851697855 1104 88-89 4.193193967990769 1104 90-91 4.1363049831434076 1104 92-93 3.8371900997522914 1104 94-95 3.4034476011254187 1104 96-97 3.144318566803663 1104 98-99 2.900578579171584 1104 100-101 2.6498924061929943 1105 1 5.812051867005298 1105 2 6.543809205545642 1105 3 6.118574249589269 1105 4 8.169263988095224 1105 5 8.085402202468519 1105 6 7.114073698557643 1105 7 7.114960363569512 1105 8 6.621911380514941 1105 9 6.264758777341175 1105 10-11 6.005153442218777 1105 12-13 5.757122648762419 1105 14-15 5.676659032559922 1105 16-17 6.091177897448695 1105 18-19 6.511631656724145 1105 20-21 7.13781913935161 1105 22-23 7.084759408701398 1105 24-25 6.706230270010966 1105 26-27 6.802617139133147 1105 28-29 6.795809787798785 1105 30-31 6.544709314060018 1105 32-33 6.212482888296524 1105 34-35 7.9450757564405805 1105 36-37 -3.157612737813748 1105 38-39 -2.3951093344325596 1105 40-41 3.9946317763030486 1105 42-43 5.727706977545893 1105 44-45 -2.977246749021587 1105 46-47 -3.740477059412017 1105 48-49 3.1323667005297438 1105 50-51 -7.634916567663072 1105 52-53 -0.47521564641837166 1105 54-55 3.847303069064285 1105 56-57 4.8482111509290675 1105 58-59 5.3776234198392 1105 60-61 5.2717644840571865 1105 62-63 5.28613349038312 1105 64-65 5.042598570735089 1105 66-67 5.007806041725482 1105 68-69 4.9815010315098345 1105 70-71 5.124499676378798 1105 72-73 4.909656497640729 1105 74-75 4.726949451921188 1105 76-77 4.580687617988188 1105 78-79 4.303074662907354 1105 80-81 4.201787408436152 1105 82-83 4.2573068027204855 1105 84-85 4.018271321187292 1105 86-87 3.950762227028779 1105 88-89 3.721908860135354 1105 90-91 3.729772870449132 1105 92-93 3.4181657121695146 1105 94-95 3.031894091918022 1105 96-97 2.8714196997161077 1105 98-99 2.61170390873088 1105 100-101 2.2906308060564546 1106 1 5.843742828178012 1106 2 6.544511437825044 1106 3 6.175763185010858 1106 4 7.543414340498966 1106 5 7.447626653856137 1106 6 6.562924969736617 1106 7 6.51652578585086 1106 8 6.002111502407889 1106 9 6.032475911010632 1106 10-11 5.61447811704668 1106 12-13 5.333837798019534 1106 14-15 5.6147412863535635 1106 16-17 5.8196306386318994 1106 18-19 6.187481432327573 1106 20-21 6.745127550642561 1106 22-23 6.52951475160776 1106 24-25 6.598659293423172 1106 26-27 6.5255446390341305 1106 28-29 6.37661167893155 1106 30-31 5.833905900394907 1106 32-33 5.7578926791584095 1106 34-35 7.354288101211182 1106 36-37 -0.06885121020327745 1106 38-39 0.38169548008279186 1106 40-41 5.006938310406493 1106 42-43 5.946910899444653 1106 44-45 -2.9090506275786225 1106 46-47 -3.8331014669458874 1106 48-49 2.5766055721510526 1106 50-51 -7.62960570074279 1106 52-53 -0.697530594357854 1106 54-55 3.3645090399813604 1106 56-57 4.199025871392276 1106 58-59 4.632719573555033 1106 60-61 4.605103439666259 1106 62-63 4.673899814949367 1106 64-65 4.697143058723727 1106 66-67 4.518482668052821 1106 68-69 4.275335769433225 1106 70-71 4.006237013796513 1106 72-73 4.132941363443283 1106 74-75 3.8541784897304385 1106 76-77 3.69343452687856 1106 78-79 3.4288100829282477 1106 80-81 3.1041829948693795 1106 82-83 3.108530769638616 1106 84-85 3.0346993096991763 1106 86-87 2.7749098159835963 1106 88-89 2.7297986197574655 1106 90-91 2.6739796649793988 1106 92-93 2.6245300630459063 1106 94-95 2.536656033970531 1106 96-97 2.238136374517503 1106 98-99 2.1155773304212895 1106 100-101 1.6767639850181597 1107 1 5.6137355560836255 1107 2 6.354473509182451 1107 3 6.0059628253117125 1107 4 7.299120974380571 1107 5 7.765840852007862 1107 6 6.3766956464601705 1107 7 6.435958048675072 1107 8 5.903465549453898 1107 9 6.043186694752919 1107 10-11 5.706772459113704 1107 12-13 5.336386001075656 1107 14-15 5.496169393822559 1107 16-17 6.249839916648213 1107 18-19 6.244590316682562 1107 20-21 6.619500152444086 1107 22-23 6.642348639419108 1107 24-25 6.312053312188656 1107 26-27 6.239878075179661 1107 28-29 6.311671519640459 1107 30-31 6.294270981214353 1107 32-33 6.121995396712013 1107 34-35 7.580490034961613 1107 36-37 -0.12386660159031138 1107 38-39 0.15321531810977085 1107 40-41 5.178459351291647 1107 42-43 5.935425757193311 1107 44-45 -3.0074084885760364 1107 46-47 -3.563598940869481 1107 48-49 3.2363387514789395 1107 50-51 -7.502957432361075 1107 52-53 -0.3052287975402006 1107 54-55 3.8603144062435035 1107 56-57 5.190158653420568 1107 58-59 5.3911566954807455 1107 60-61 5.490123489529925 1107 62-63 5.337282777814762 1107 64-65 5.4035457242574285 1107 66-67 5.089267233036194 1107 68-69 4.937484094913577 1107 70-71 4.901683245219592 1107 72-73 4.692695793265493 1107 74-75 4.7031052209663375 1107 76-77 4.680084657462919 1107 78-79 4.639716561299595 1107 80-81 4.4601181569530315 1107 82-83 4.274214532879892 1107 84-85 4.199335175573658 1107 86-87 3.836293598871558 1107 88-89 3.708077377237645 1107 90-91 3.6861206619349822 1107 92-93 3.3581168777007555 1107 94-95 3.130867004304336 1107 96-97 2.8917750724547213 1107 98-99 2.6223263300071205 1107 100-101 2.2749590146597045 1108 1 6.073229106345934 1108 2 6.084252023678605 1108 3 6.627171054066952 1108 4 7.865820540454909 1108 5 7.916963544294646 1108 6 7.329114380243222 1108 7 7.430879750275455 1108 8 6.506990844458226 1108 9 4.905196930212707 1108 10-11 5.8495288788296875 1108 12-13 5.418299327665803 1108 14-15 5.924230218693552 1108 16-17 5.955734073558073 1108 18-19 6.286667827647204 1108 20-21 7.264230835343785 1108 22-23 7.150928947331277 1108 24-25 6.719837686443714 1108 26-27 7.0759747247573515 1108 28-29 7.161158073799914 1108 30-31 6.951032227703607 1108 32-33 6.97141436469424 1108 34-35 8.846626925573272 1108 36-37 -2.728756838651254 1108 38-39 -1.9744596755722288 1108 40-41 4.237422129754162 1108 42-43 6.400273028862557 1108 44-45 -2.395303931191563 1108 46-47 -3.359460008184417 1108 48-49 3.7048282833135957 1108 50-51 -7.535322324232153 1108 52-53 0.16090910873113806 1108 54-55 4.464048434248227 1108 56-57 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-12.094811399058813 2313 70-71 -11.511039062679291 2313 72-73 -11.007841762760577 2313 74-75 -10.47657538119866 2313 76-77 -9.917774436605594 2313 78-79 -9.412134983414184 2313 80-81 -8.89349557758783 2313 82-83 -8.406433479904582 2313 84-85 -7.904775268106606 2313 86-87 -7.37566689267023 2313 88-89 -6.860079929569658 2313 90-91 -6.328499729526363 2313 92-93 -5.78248699339815 2313 94-95 -5.258580231876569 2313 96-97 -4.661399221812582 2313 98-99 -4.059982086056578 2313 100-101 -3.4073608447740984 2314 1 -19.216956365060557 2314 2 -17.9787309747758 2314 3 -18.307141156133977 2314 4 -20.08240202523288 2314 5 -20.12863150980118 2314 6 -20.76362132300253 2314 7 -20.706678209539074 2314 8 -21.173070979343937 2314 9 -22.074323935320525 2314 10-11 -22.339613315915287 2314 12-13 -22.17192478361704 2314 14-15 -22.18241584003906 2314 16-17 -21.39608200063049 2314 18-19 -20.858811306819565 2314 20-21 -20.317492503141533 2314 22-23 -20.15181448388974 2314 24-25 -19.841986115720122 2314 26-27 -19.44199281774651 2314 28-29 -19.21712631569004 2314 30-31 -19.00762310459933 2314 32-33 -18.581715465290305 2314 34-35 -16.157237062061967 2314 36-37 -14.579800115312768 2314 38-39 -13.769668330904532 2314 40-41 -14.72586998771106 2314 42-43 -14.539835162791228 2314 44-45 -12.880813977559415 2314 46-47 -13.252041151924757 2314 48-49 -12.812752860426741 2314 50-51 -11.263297594711288 2314 52-53 -13.440327367312138 2314 54-55 -14.676021117529206 2314 56-57 -14.773505822806722 2314 58-59 -14.37208257780878 2314 60-61 -14.022825872665667 2314 62-63 -13.524332993348748 2314 64-65 -13.019337924756874 2314 66-67 -12.582746682696316 2314 68-69 -12.09827160667127 2314 70-71 -11.52622552942285 2314 72-73 -11.028603008435319 2314 74-75 -10.500412366973363 2314 76-77 -9.92930846198045 2314 78-79 -9.42213113873906 2314 80-81 -8.914833524531312 2314 82-83 -8.43046269943553 2314 84-85 -7.928612253881308 2314 86-87 -7.400080579713675 2314 88-89 -6.88314798031937 2314 90-91 -6.351567780276074 2314 92-93 -5.799788031460434 2314 94-95 -5.258580231876569 2314 96-97 -4.665820598206277 2314 98-99 -4.06940154011271 2314 100-101 -3.4188948701489545 2315 1 -19.216956365060557 2315 2 -17.9787309747758 2315 3 -18.307141156133977 2315 4 -16.166280440007796 2315 5 -20.12863150980118 2315 6 -20.76362132300253 2315 7 -20.706678209539074 2315 8 -21.173070979343937 2315 9 -18.496794116159307 2315 10-11 -17.45965948751975 2315 12-13 -14.649800889811731 2315 14-15 -14.387494716530018 2315 16-17 -18.244485232642802 2315 18-19 -20.858811306819565 2315 20-21 -20.317492503141533 2315 22-23 -20.15181448388974 2315 24-25 -19.841986115720122 2315 26-27 -19.44199281774651 2315 28-29 -19.21712631569004 2315 30-31 -19.00762310459933 2315 32-33 -18.581715465290305 2315 34-35 -16.157237062061967 2315 36-37 -14.579800115312768 2315 38-39 -13.769668330904532 2315 40-41 -14.72586998771106 2315 42-43 -14.539835162791228 2315 44-45 -12.880813977559415 2315 46-47 -13.252041151924757 2315 48-49 -12.812752860426741 2315 50-51 -11.263297594711288 2315 52-53 -13.440327367312138 2315 54-55 -14.676021117529206 2315 56-57 -14.773505822806722 2315 58-59 -14.37208257780878 2315 60-61 -14.022825872665667 2315 62-63 -13.524332993348748 2315 64-65 -13.019337924756874 2315 66-67 -12.582746682696316 2315 68-69 -12.09827160667127 2315 70-71 -11.52622552942285 2315 72-73 -11.028603008435319 2315 74-75 -10.500412366973363 2315 76-77 -9.92930846198045 2315 78-79 -9.42213113873906 2315 80-81 -8.914833524531312 2315 82-83 -8.43046269943553 2315 84-85 -7.928612253881308 2315 86-87 -7.400080579713675 2315 88-89 -6.88314798031937 2315 90-91 -6.351567780276074 2315 92-93 -5.799788031460434 2315 94-95 -5.258580231876569 2315 96-97 -4.665820598206277 2315 98-99 -4.06940154011271 2315 100-101 -3.4188948701489545 2316 1 -19.216956365060557 2316 2 -17.9787309747758 2316 3 -18.307141156133977 2316 4 -20.08240202523288 2316 5 -20.12863150980118 2316 6 -20.76362132300253 2316 7 -20.706678209539074 2316 8 -21.173070979343937 2316 9 -22.074323935320525 2316 10-11 -22.339613315915287 2316 12-13 -22.17192478361704 2316 14-15 -22.18241584003906 2316 16-17 -21.39608200063049 2316 18-19 -20.858811306819565 2316 20-21 -20.317492503141533 2316 22-23 -20.15181448388974 2316 24-25 -19.841986115720122 2316 26-27 -19.44199281774651 2316 28-29 -19.21712631569004 2316 30-31 -19.00762310459933 2316 32-33 -18.581715465290305 2316 34-35 -16.157237062061967 2316 36-37 -14.579800115312768 2316 38-39 -13.769668330904532 2316 40-41 -14.72586998771106 2316 42-43 -14.539835162791228 2316 44-45 -12.880813977559415 2316 46-47 -13.252041151924757 2316 48-49 -12.812752860426741 2316 50-51 -11.263297594711288 2316 52-53 -13.440327367312138 2316 54-55 -14.676021117529206 2316 56-57 -14.773505822806722 2316 58-59 -14.37208257780878 2316 60-61 -14.022825872665667 2316 62-63 -13.524332993348748 2316 64-65 -13.019337924756874 2316 66-67 -12.582746682696316 2316 68-69 -12.09827160667127 2316 70-71 -11.52622552942285 2316 72-73 -11.028603008435319 2316 74-75 -10.500412366973363 2316 76-77 -9.92930846198045 2316 78-79 -9.42213113873906 2316 80-81 -8.914833524531312 2316 82-83 -8.43046269943553 2316 84-85 -7.928612253881308 2316 86-87 -7.400080579713675 2316 88-89 -6.88314798031937 2316 90-91 -6.351567780276074 2316 92-93 -5.799788031460434 2316 94-95 -5.258580231876569 2316 96-97 -4.665820598206277 2316 98-99 -4.06940154011271 2316 100-101 -3.4188948701489545 >>END_MODULE >>Per sequence quality scores fail #Quality Count 2 484074.0 3 38467.0 4 63090.0 5 46889.0 6 43970.0 7 54266.0 8 67242.0 9 75348.0 10 74577.0 11 67042.0 12 57594.0 13 51312.0 14 49843.0 15 52225.0 16 55515.0 17 57967.0 18 59831.0 19 61981.0 20 63638.0 21 65591.0 22 68311.0 23 69705.0 24 71582.0 25 73011.0 26 74362.0 27 76349.0 28 76481.0 29 73803.0 30 69915.0 31 63376.0 32 55442.0 33 44400.0 34 30328.0 35 15825.0 36 4628.0 37 494.0 38 11.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.604273264254125 19.619223501525486 13.789297324569127 23.98720590965126 2 15.841271059122622 20.78203231355114 40.42553191489361 22.95116471243262 3 18.982226860052382 26.05730561519495 30.062089443024355 24.898378081728314 4 12.879395393610165 17.294784476895842 38.74825975051742 31.077560378976578 5 13.3420377172491 37.25208892104966 35.665898478568074 13.739974883133158 6 30.487562633651237 37.30010291418589 18.433489703967513 13.77884474819536 7 26.559843040928726 32.26388360657151 23.506881808401488 17.669391544098275 8 25.120301841102986 35.49229452649186 21.55260072748075 17.834802904924405 9 26.041284220817502 16.31391784696012 21.69420976110203 35.950588171120344 10-11 24.337958195172188 26.581718418516626 29.554377528359847 19.525945857951335 12-13 24.790269358721332 24.831057639704078 29.19110132154874 21.187571680025847 14-15 22.23071402484929 26.33070185114949 26.701384417752156 24.737199706249065 16-17 22.09979666093429 29.205671416764133 27.133967834620627 21.560564087680945 18-19 21.921397421522126 28.26549350949748 28.816935503273893 20.996173565706496 20-21 22.693322300641803 27.402375763705027 28.83870865793341 21.065593277719756 22-23 22.387310790193997 27.12215998324783 28.773609158352905 21.716920068205273 24-25 22.153975368525785 27.19171046339207 28.85081694758696 21.80349722049518 26-27 22.097227680158685 27.376798779917287 28.79617414248021 21.729799397443813 28-29 22.11815430506673 27.526522893876145 28.76231641460346 21.593006386453663 30-31 22.347653796883073 27.25638618139926 28.684668014019145 21.71129200769853 32-33 22.209449303286537 27.379904706080353 28.578890249493643 21.831755741139464 34-35 22.51643555688246 27.275283222115533 28.597465525171266 21.610815695830745 36-37 23.028082342050205 27.236901952600345 28.178928611561492 21.55608709378796 38-39 22.788055057840808 27.266825935386034 28.18619508723775 21.75892391953541 40-41 22.790218925956403 27.15829073979919 28.345677747121268 21.705812587123138 42-43 22.81638001173815 27.13284665508761 28.123604044068024 21.927169289106217 44-45 23.08212775215594 27.27733507386501 27.91444086084061 21.726096313138438 46-47 22.44702274970656 27.12680236994584 28.27765889674774 22.14851598359986 48-49 22.920663863231695 27.223034028273347 27.82700388533347 22.02929822316149 50-51 23.033222008835207 27.42027489997968 27.73524240257428 21.81126068861084 52-53 22.17004710664666 27.442281517244922 28.240897851217838 22.14677352489058 54-55 21.9489723997517 27.40134210884001 28.477588781475966 22.172096709932323 56-57 22.08881066289101 27.59589304220627 28.193063063439705 22.122233231463017 58-59 22.106853516811952 27.513215452173622 28.238672922787543 22.14125810822688 60-61 22.083233156945084 27.544787598824495 28.215428642121697 22.156550602108723 62-63 22.04439757890771 27.52817861679531 28.20194587885907 22.225477925437918 64-65 21.90419314732558 27.408453479123324 28.14925441535926 22.538098958191842 66-67 21.996440132673747 27.544861486966266 27.962125383966878 22.496572996393102 68-69 21.989953028791845 27.49220077249045 28.04033887543885 22.477507323278857 70-71 21.94895991032369 27.250785625300978 27.954416080634843 22.845838383740492 72-73 22.080037535779727 27.462884619173582 27.790887401366625 22.666190443680065 74-75 22.111578861923217 27.632049921819444 27.62902313405987 22.627348082197464 76-77 22.093904762604033 27.443688898026014 27.70512182765343 22.757284511716527 78-79 22.096122554308927 27.51116767334587 27.59634142434024 22.796368348004965 80-81 22.206199834507014 27.549627732955802 27.40667055167324 22.837501880863947 82-83 22.241921474760975 27.530694917570187 27.50255097011643 22.72483263755241 84-85 22.21246234586879 27.646452101318985 27.377773773209956 22.763311779602272 86-87 22.093142416138182 27.653519571007646 27.293622701842878 22.959715311011294 88-89 22.08238452793548 27.546029299801774 27.226677816209914 23.14490835605283 90-91 22.160890046246262 27.526571946233652 27.210819748433053 23.101718259087033 92-93 22.213395074497384 27.358469170995676 27.245923037504 23.182212717002944 94-95 22.04101237032105 27.331548963474393 27.252805175758287 23.374633490446268 96-97 22.104777922561006 27.255929329557304 27.08774243900658 23.55155030887511 98-99 22.01999527024964 27.267876565362826 26.969966085229373 23.742162079158156 100-101 22.42814282345138 27.412151753452413 26.850924726787863 23.308780696308347 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 30198.0 1 32203.5 2 23792.5 3 14530.0 4 18900.0 5 23473.0 6 24757.0 7 23754.0 8 21679.5 9 19596.0 10 17560.0 11 15282.0 12 13114.0 13 11308.0 14 9717.0 15 8584.0 16 7877.0 17 7380.5 18 7237.0 19 7499.5 20 8078.5 21 8939.0 22 10198.5 23 11849.0 24 13982.0 25 16765.0 26 20113.0 27 24071.5 28 28683.0 29 33782.0 30 39298.5 31 44537.0 32 49097.0 33 53158.5 34 56569.0 35 59386.5 36 62287.5 37 65155.5 38 66961.0 39 68586.5 40 69878.5 41 70457.5 42 71205.0 43 72224.0 44 73201.0 45 73929.5 46 74163.5 47 73542.0 48 72746.0 49 71568.0 50 69362.0 51 66459.0 52 63504.0 53 60580.0 54 56924.0 55 52510.0 56 47594.0 57 42374.5 58 37557.5 59 32862.0 60 28063.5 61 23533.0 62 19477.0 63 15756.0 64 12469.5 65 9798.0 66 7628.0 67 5883.0 68 4469.0 69 3459.0 70 2687.0 71 2011.0 72 1504.0 73 1117.0 74 863.0 75 652.0 76 465.0 77 356.5 78 254.0 79 179.0 80 125.5 81 74.0 82 44.0 83 37.5 84 28.0 85 17.5 86 13.0 87 10.5 88 7.5 89 6.0 90 5.0 91 3.5 92 1.5 93 0.0 94 0.5 95 1.5 96 1.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 14.54208587809159 2 21.5381017171144 3 17.28572677889025 4 22.54656831341253 5 22.428446787350747 6 16.249356819341994 7 15.082744047655364 8 12.11632367087861 9 3.0608281116215883 10-11 6.550660264349793 12-13 3.5674815994403057 14-15 7.44718800399433 16-17 11.563381513411715 18-19 14.624189287304986 20-21 15.055857570821054 22-23 12.977951868732166 24-25 15.539488750185582 26-27 13.05377091989579 28-29 13.05649617549019 30-31 13.92060557619835 32-33 12.390659288138833 34-35 19.344799744558134 36-37 23.986113399105548 38-39 23.179030988596637 40-41 18.238834078711076 42-43 18.18577294553353 44-45 23.441326670693538 46-47 12.846122713785116 48-49 21.655430071771846 50-51 21.24289959060153 52-53 11.303750073724265 54-55 12.915515042800749 56-57 12.436439514579101 58-59 13.457962932456372 60-61 12.87093474233115 62-63 12.822429260296483 64-65 13.90793517145722 66-67 12.213151595393096 68-69 13.25621266755746 70-71 11.86016998273327 72-73 11.227808996190744 74-75 11.305966886110754 76-77 11.362322731275562 78-79 11.771050057250704 80-81 11.73911982379392 82-83 10.24905582096291 84-85 10.16463391072144 86-87 10.296219012928693 88-89 11.71422644433462 90-91 9.20064592625133 92-93 11.219287488026163 94-95 11.333422819337926 96-97 10.87946438558706 98-99 10.732870039882284 100-101 10.832850312285819 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2458485.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 98.41087513293962 #Duplication Level Percentage of deduplicated Percentage of total 1 99.40674264176735 97.82704537491232 2 0.42691707881265145 0.8402656667030238 3 0.08666683228495713 0.25586876430487004 4 0.02793323314623594 0.1099573567685406 5 0.013156378935632443 0.06473653823180807 6 0.009322187561486133 0.05504427816475528 7 0.005706236777256899 0.039308902847593155 8 0.00523853461193036 0.04124230204594088 9 0.0027703108949393446 0.024536584760516823 >10 0.013876138576178745 0.23818813751455775 >50 9.225645765747244E-4 0.06622956770629627 >100 5.821764253075741E-4 0.08778776865861172 >500 4.142140737339926E-5 0.037502115929458804 >1k 1.2426422212019774E-4 0.3122866414517217 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 4426 0.1800295710569721 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 4.067545663284503E-5 0.0 0.0 0.0 4.067545663284503E-5 7 4.067545663284503E-5 0.0 0.0 0.0 4.067545663284503E-5 8 4.067545663284503E-5 0.0 0.0 0.0 4.067545663284503E-5 9 8.135091326569005E-5 0.0 0.0 8.135091326569005E-5 4.067545663284503E-5 10-11 8.135091326569005E-5 0.0 0.0 8.135091326569005E-5 4.067545663284503E-5 12-13 8.135091326569005E-5 2.0337728316422514E-5 0.0 8.135091326569005E-5 1.423640982149576E-4 14-15 8.135091326569005E-5 4.067545663284503E-5 0.0 8.135091326569005E-5 1.830395548478026E-4 16-17 8.135091326569005E-5 4.067545663284503E-5 0.0 8.135091326569005E-5 2.2371501148064763E-4 18-19 8.135091326569005E-5 4.067545663284503E-5 0.0 8.135091326569005E-5 2.4405273979707015E-4 20-21 8.135091326569005E-5 4.067545663284503E-5 0.0 8.135091326569005E-5 3.660791096956052E-4 22-23 8.135091326569005E-5 8.135091326569005E-5 0.0 1.2202636989853507E-4 4.4743002296129526E-4 24-25 8.135091326569005E-5 1.2202636989853507E-4 0.0 2.847281964299152E-4 4.881054795941403E-4 26-27 8.135091326569005E-5 1.2202636989853507E-4 0.0 4.0675456632845023E-4 4.881054795941403E-4 28-29 8.135091326569005E-5 1.2202636989853507E-4 0.0 8.135091326569005E-4 4.881054795941403E-4 30-31 8.135091326569005E-5 1.2202636989853507E-4 0.0 0.0021354614732243637 4.881054795941403E-4 32-33 8.135091326569005E-5 1.2202636989853507E-4 0.0 0.005816590298496838 4.881054795941403E-4 34-35 8.135091326569005E-5 1.2202636989853507E-4 0.0 0.011856895608474324 4.881054795941403E-4 36-37 8.135091326569005E-5 1.2202636989853507E-4 0.0 0.021334277003927215 4.881054795941403E-4 38-39 8.135091326569005E-5 1.2202636989853507E-4 0.0 0.03597744139175142 4.881054795941403E-4 40-41 8.135091326569005E-5 1.2202636989853507E-4 0.0 0.05173918083697887 4.881054795941403E-4 42-43 8.135091326569005E-5 1.2202636989853507E-4 2.0337728316422514E-5 0.06750092028220632 4.881054795941403E-4 44-45 8.135091326569005E-5 1.2202636989853507E-4 4.067545663284503E-5 0.08669973581290917 5.084432079105628E-4 46-47 8.135091326569005E-5 1.2202636989853507E-4 4.067545663284503E-5 0.11212189620843731 5.491186645434078E-4 48-49 8.135091326569005E-5 1.2202636989853507E-4 4.067545663284503E-5 0.13902871077106427 5.694563928598304E-4 50-51 8.135091326569005E-5 1.2202636989853507E-4 4.067545663284503E-5 0.17268765113474355 5.694563928598304E-4 52-53 8.135091326569005E-5 1.2202636989853507E-4 4.067545663284503E-5 0.2144613450966754 5.694563928598304E-4 54-55 8.135091326569005E-5 1.2202636989853507E-4 4.067545663284503E-5 0.2617465634323577 5.694563928598304E-4 56-57 8.135091326569005E-5 1.2202636989853507E-4 4.067545663284503E-5 0.31610931122215513 6.101318494926754E-4 58-59 8.135091326569005E-5 1.2202636989853507E-4 4.067545663284503E-5 0.37643101340866425 6.508073061255204E-4 60-61 8.135091326569005E-5 1.2202636989853507E-4 4.067545663284503E-5 0.4413897176513178 6.508073061255204E-4 62-63 8.135091326569005E-5 1.2202636989853507E-4 4.067545663284503E-5 0.506429772807237 7.321582193912104E-4 64-65 8.135091326569005E-5 1.2202636989853507E-4 4.067545663284503E-5 0.5758017640945541 7.931714043404779E-4 66-67 8.135091326569005E-5 1.2202636989853507E-4 4.067545663284503E-5 0.6528207412288463 8.135091326569005E-4 68-69 8.135091326569005E-5 1.2202636989853507E-4 4.067545663284503E-5 0.7398662184231346 8.135091326569005E-4 70-71 8.135091326569005E-5 1.2202636989853507E-4 4.067545663284503E-5 0.8335417950485766 8.135091326569005E-4 72-73 8.135091326569005E-5 1.2202636989853507E-4 4.067545663284503E-5 0.932281466024808 8.74522317606168E-4 74-75 8.135091326569005E-5 1.2202636989853507E-4 4.067545663284503E-5 1.0319770102319112 9.15197774239013E-4 76-77 8.135091326569005E-5 1.2202636989853507E-4 4.067545663284503E-5 1.1430413445678944 9.355355025554356E-4 78-79 1.2202636989853507E-4 1.2202636989853507E-4 4.067545663284503E-5 1.2652100785646445 9.355355025554356E-4 80-81 1.2202636989853507E-4 1.2202636989853507E-4 4.067545663284503E-5 1.4008017132502335 9.355355025554356E-4 82-83 1.2202636989853507E-4 1.2202636989853507E-4 4.067545663284503E-5 1.5421285873210535 9.355355025554356E-4 84-85 1.2202636989853507E-4 1.2202636989853507E-4 4.067545663284503E-5 1.6848994401023396 9.355355025554356E-4 86-87 1.2202636989853507E-4 1.2202636989853507E-4 4.067545663284503E-5 1.8303141975647605 9.762109591882806E-4 88-89 1.2202636989853507E-4 1.2202636989853507E-4 4.067545663284503E-5 1.9882976711267304 0.001037224144137548 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1855 0.0 54.63355 1 GTATCAA 3645 0.0 46.47817 1 TCAACGC 4455 0.0 38.229904 4 ATCAACG 4420 0.0 37.403854 3 TATCAAC 4505 0.0 36.25356 2 CAACGCA 4665 0.0 35.8881 5 AACGCAG 5090 0.0 33.36555 6 ACGCAGA 5800 0.0 28.517832 7 GTACATG 4095 0.0 28.07296 1 CGCAGAG 5930 0.0 27.65955 8 TACATGG 4335 0.0 26.395943 2 GCAGAGT 5960 0.0 24.840712 9 ACATGGG 4525 0.0 24.72858 3 AGAGTAC 5345 0.0 20.741617 10-11 CATGGGG 3455 0.0 20.709732 4 GAGTACT 3850 0.0 19.902855 12-13 TAACGCA 380 3.6379788E-12 19.890324 4 CAGAGTA 5805 0.0 19.707924 10-11 GTATTAG 735 0.0 19.207815 1 AGTACTT 4375 0.0 17.934023 12-13 >>END_MODULE