FastQCFastQC Report
Sat 18 Jun 2016
SRR1930909_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1930909_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2458485
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43180.17563662174062483No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37060.15074324228132366No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA26500.048.026531
GTATCAA50550.043.1205021
TCAACGC63250.033.7012144
ATCAACG63000.033.684923
CAACGCA63700.033.3784265
AACGCAG67950.031.6399656
TATCAAC67850.031.5569692
ACGCAGA77100.027.7003577
CGCAGAG79300.026.7523298
GTACATG60450.023.9604991
GTGGTAT16650.023.1027161
GCAGAGT91200.022.6891849
TACATGG64050.022.2367172
TATCACG3007.2759576E-1222.1551842
ACATGGG61200.022.1086393
AGAGTAC83800.021.20972410-11
TGGTATC16100.020.6414742
TAACGCA3001.6916601E-1020.5722524
ATAACGC2201.3527224E-619.4217513
ATCACGC3455.2750693E-1119.2653773
Produced by FastQC (version 0.11.5)