##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1930909_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2458485 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.240327274724066 31.0 31.0 33.0 30.0 34.0 2 31.35733632704694 31.0 31.0 34.0 30.0 34.0 3 31.437419386329385 31.0 31.0 34.0 28.0 34.0 4 35.301105355534 37.0 35.0 37.0 33.0 37.0 5 35.08647602080143 35.0 35.0 37.0 32.0 37.0 6 35.13082203064082 37.0 35.0 37.0 32.0 37.0 7 35.01490186029201 36.0 35.0 37.0 32.0 37.0 8 34.99139063284909 36.0 35.0 37.0 32.0 37.0 9 36.50038743372443 38.0 35.0 39.0 32.0 39.0 10-11 36.43080433681719 38.0 35.0 39.0 32.0 39.0 12-13 36.32679068613394 38.0 35.0 39.0 32.0 39.0 14-15 37.341881280544726 39.0 36.0 41.0 32.0 41.0 16-17 37.30380478221343 39.0 36.0 41.0 32.0 41.0 18-19 37.29586005202391 39.0 36.0 40.0 32.0 41.0 20-21 37.262685963103294 39.0 36.0 40.0 31.0 41.0 22-23 37.13566607077123 39.0 36.0 40.0 31.0 41.0 24-25 37.012277683207344 39.0 36.0 40.0 31.0 41.0 26-27 36.73551984250463 39.0 35.5 40.0 30.0 41.0 28-29 36.543106425298504 38.0 35.0 40.0 30.0 41.0 30-31 36.30157800434007 38.0 35.0 40.0 30.0 41.0 32-33 36.05631455957632 38.0 34.5 40.0 29.5 41.0 34-35 36.24980079195114 38.0 35.0 40.0 30.0 41.0 36-37 36.29360805536743 38.0 35.0 40.0 30.0 41.0 38-39 36.25061023353814 38.0 35.0 40.0 30.0 41.0 40-41 36.15235704102324 38.0 35.0 40.0 30.0 41.0 42-43 36.01085810977085 38.0 35.0 40.0 29.0 41.0 44-45 35.8235126510839 38.0 34.5 40.0 28.5 41.0 46-47 35.59993695304222 38.0 34.0 40.0 28.0 41.0 48-49 35.394577351498995 38.0 34.0 40.0 27.0 41.0 50-51 35.12416386514459 38.0 34.0 40.0 26.5 41.0 52-53 34.911192462024374 38.0 33.0 40.0 26.0 41.0 54-55 34.741187153877284 38.0 33.0 40.0 26.0 41.0 56-57 34.47964091706885 37.5 33.0 40.0 26.0 41.0 58-59 34.23959409961826 37.0 33.0 40.0 25.0 41.0 60-61 33.931871864176514 37.0 32.5 40.0 24.5 41.0 62-63 33.62422955600705 36.0 32.0 39.0 23.5 41.0 64-65 33.32338737067747 36.0 32.0 39.0 23.0 40.5 66-67 32.98559153299695 35.5 31.0 39.0 22.5 40.0 68-69 32.60889775613843 35.0 31.0 38.5 22.0 40.0 70-71 32.20207424491099 35.0 31.0 38.0 21.5 40.0 72-73 31.78285610853839 35.0 30.5 37.5 20.5 39.5 74-75 31.25350429227756 34.0 30.0 37.0 19.0 39.0 76-77 29.89083581962062 33.0 28.5 35.5 18.0 38.0 78-79 30.647622824625735 34.0 29.5 36.0 18.5 38.5 80-81 30.642831865966237 34.0 30.0 36.0 18.5 37.5 82-83 30.35474001265007 34.0 30.0 35.0 18.0 37.0 84-85 30.022323097354672 34.0 29.5 35.0 16.5 37.0 86-87 29.670732585311686 34.0 29.0 35.0 12.0 36.0 88-89 29.311746665121 34.0 29.0 35.0 7.5 36.0 90-91 28.9598905423462 34.0 29.0 35.0 4.5 35.5 92-93 28.585090004616664 33.5 29.0 35.0 2.0 35.0 94-95 28.152617160568397 33.0 28.0 35.0 2.0 35.0 96-97 27.678517460956648 33.0 27.0 35.0 2.0 35.0 98-99 27.164800069961785 33.0 26.5 35.0 2.0 35.0 100-101 25.92683563251352 32.0 24.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.5193914610980492 1101 2 0.5214409386502972 1101 3 0.5381290114331634 1101 4 0.41135337213838596 1101 5 0.5207426990997917 1101 6 0.46473762669617713 1101 7 0.5641922253137537 1101 8 0.5324986870052442 1101 9 0.5914367088036485 1101 10-11 0.5787485148168088 1101 12-13 0.6396905752803335 1101 14-15 0.8195240067722622 1101 16-17 0.80664306155807 1101 18-19 0.7338209992013276 1101 20-21 0.6687175234252365 1101 22-23 0.7614258858870713 1101 24-25 0.6243534558437958 1101 26-27 0.6412417876949519 1101 28-29 0.6624078117351146 1101 30-31 0.800660863469453 1101 32-33 0.8441397393707817 1101 34-35 0.702110411604771 1101 36-37 0.8407022710061298 1101 38-39 0.8647867056397089 1101 40-41 0.8225197784239171 1101 42-43 0.829089806224502 1101 44-45 0.8650885404847699 1101 46-47 0.8044545869300492 1101 48-49 0.9073765711294257 1101 50-51 0.9630370349381465 1101 52-53 0.8389114278294372 1101 54-55 0.8098917321577446 1101 56-57 1.0618200908738231 1101 58-59 1.0700963071919105 1101 60-61 1.0372983114807042 1101 62-63 0.9248605626172193 1101 64-65 0.9122904786412036 1101 66-67 1.036418085793585 1101 68-69 0.792518481721693 1101 70-71 0.8899798316008614 1101 72-73 0.9891708263437309 1101 74-75 1.0302816138338926 1101 76-77 0.9211505272614957 1101 78-79 0.8865287672491 1101 80-81 0.7959312602251956 1101 82-83 0.9775146299924096 1101 84-85 1.084215798185916 1101 86-87 1.0368717004538759 1101 88-89 0.7760404111654431 1101 90-91 0.7539288067589354 1101 92-93 0.9959673654570871 1101 94-95 0.865725316214629 1101 96-97 0.8194550228343154 1101 98-99 0.935476725891565 1101 100-101 1.2694502399023548 1102 1 0.49749642664189153 1102 2 0.5473044951334423 1102 3 0.5934541670362528 1102 4 0.23932388358894485 1102 5 0.3822220826304843 1102 6 0.4447025142800882 1102 7 0.4542343973134919 1102 8 0.43077558158644536 1102 9 0.6295423603556216 1102 10-11 0.6544046238417067 1102 12-13 0.5653102313732532 1102 14-15 0.7893499742099763 1102 16-17 0.7853933472489558 1102 18-19 0.694921100737524 1102 20-21 0.7116950577090151 1102 22-23 0.7113332047888079 1102 24-25 0.7169263153321808 1102 26-27 0.7836404501191012 1102 28-29 0.7898781847590328 1102 30-31 0.8328777827399492 1102 32-33 0.8567863517331347 1102 34-35 0.9424257968420875 1102 36-37 0.9820797088287705 1102 38-39 1.0068337013966087 1102 40-41 0.8806926552725187 1102 42-43 0.8976910566493217 1102 44-45 0.8748988942374609 1102 46-47 0.9986867635813397 1102 48-49 0.8903424817754626 1102 50-51 0.8760617042804739 1102 52-53 0.9644682406776681 1102 54-55 0.791241936157661 1102 56-57 0.6938591555367353 1102 58-59 0.7790724085587115 1102 60-61 0.7895810385310256 1102 62-63 0.7453174475313062 1102 64-65 0.6355228614449402 1102 66-67 0.7442839740043539 1102 68-69 0.7481008553728188 1102 70-71 0.6483487807729134 1102 72-73 0.7207725958246982 1102 74-75 0.8959596471185876 1102 76-77 1.0262977232557695 1102 78-79 1.013375170378982 1102 80-81 0.8654776968504692 1102 82-83 0.9730449020261354 1102 84-85 1.1003021638093493 1102 86-87 1.0556948360080334 1102 88-89 1.118790022748339 1102 90-91 1.1313182413237328 1102 92-93 1.1063017104190713 1102 94-95 0.9817439035348876 1102 96-97 1.0584543924768433 1102 98-99 0.9884580787205941 1102 100-101 1.3531944197708867 1103 1 0.3552600165819122 1103 2 0.5213845439984475 1103 3 0.4162228030047572 1103 4 0.2665263705174752 1103 5 0.29203799429811994 1103 6 0.35486648674575605 1103 7 0.39149882712080597 1103 8 0.39034576062448423 1103 9 0.4985356883372347 1103 10-11 0.4936120518686167 1103 12-13 0.48679974390899616 1103 14-15 0.6870572943209794 1103 16-17 0.6795814997029979 1103 18-19 0.5945010391273513 1103 20-21 0.659250314493427 1103 22-23 0.6672816747288906 1103 24-25 0.584632159597156 1103 26-27 0.8278383704558365 1103 28-29 0.841084838180052 1103 30-31 0.7526279296847278 1103 32-33 0.742644675632711 1103 34-35 0.6721656299264609 1103 36-37 0.7756724043293133 1103 38-39 0.874143114649705 1103 40-41 0.7639216698936266 1103 42-43 0.8347648207988883 1103 44-45 0.9599728017061793 1103 46-47 1.0155432285973944 1103 48-49 0.919876549505716 1103 50-51 0.8935226690060531 1103 52-53 0.6640390510024474 1103 54-55 0.9499042566141682 1103 56-57 0.8759436643572656 1103 58-59 0.8596344073149069 1103 60-61 0.8778423468813301 1103 62-63 0.8580348895574303 1103 64-65 0.9462652290602733 1103 66-67 0.7607267755866616 1103 68-69 0.8405900787109815 1103 70-71 0.6518308390065499 1103 72-73 0.5916957844369648 1103 74-75 0.8669178977377143 1103 76-77 0.9234521556138198 1103 78-79 0.9915250566194054 1103 80-81 0.879856276097005 1103 82-83 0.8510411467231123 1103 84-85 1.0492511140392118 1103 86-87 1.1336079897203781 1103 88-89 1.0888945860130832 1103 90-91 1.1183051182373234 1103 92-93 1.3302348144986347 1103 94-95 1.251577610663908 1103 96-97 1.106898995785638 1103 98-99 1.367243175917423 1103 100-101 1.704107780784156 1104 1 0.389553638831984 1104 2 0.41494964211610963 1104 3 0.3381246567692351 1104 4 0.2500837531212312 1104 5 0.3796005042486783 1104 6 0.33187833761181196 1104 7 0.48675072436159184 1104 8 0.4108347364206324 1104 9 0.5982993241813119 1104 10-11 0.5272357715627649 1104 12-13 0.5992396833279159 1104 14-15 0.5504273818042904 1104 16-17 0.6154643545247822 1104 18-19 0.6904554971839403 1104 20-21 0.6608015818380224 1104 22-23 0.590960137831182 1104 24-25 0.585050185323702 1104 26-27 0.8254065547790574 1104 28-29 0.7519106431041038 1104 30-31 0.7829188739574917 1104 32-33 0.7883544008542813 1104 34-35 0.7549077807178151 1104 36-37 0.6210886134401505 1104 38-39 0.653369195536051 1104 40-41 0.6726206056425852 1104 42-43 0.5820372437560124 1104 44-45 0.6648837875365814 1104 46-47 0.7247434686356016 1104 48-49 0.6824877406749152 1104 50-51 0.711078524836168 1104 52-53 0.7960094462898653 1104 54-55 0.8073313572546752 1104 56-57 0.9416663712371403 1104 58-59 0.7962577881796093 1104 60-61 0.7838008463975754 1104 62-63 0.7724465714169924 1104 64-65 0.5921406949507642 1104 66-67 0.788222172813164 1104 68-69 0.7350479720082816 1104 70-71 0.7659249192887145 1104 72-73 0.6232346824656361 1104 74-75 0.858848041719817 1104 76-77 0.947508638074293 1104 78-79 1.1480507892870513 1104 80-81 1.0593044331505794 1104 82-83 1.13662701549406 1104 84-85 1.1029519072267 1104 86-87 1.1148201010360594 1104 88-89 1.184965797348763 1104 90-91 1.1146441273040715 1104 92-93 1.3036309877670327 1104 94-95 1.2036818231712054 1104 96-97 1.2201692714621757 1104 98-99 1.2382863215610165 1104 100-101 1.394080470314325 1105 1 0.17854902753465396 1105 2 0.38024715535108555 1105 3 0.3481105275110714 1105 4 0.15742714864772012 1105 5 0.22158271525547235 1105 6 0.24046741467432753 1105 7 0.22623416865857138 1105 8 0.3708507282781923 1105 9 0.3557536573746205 1105 10-11 0.3443538393224159 1105 12-13 0.3313275169689618 1105 14-15 0.49540494878188923 1105 16-17 0.42044030049537895 1105 18-19 0.45678913003987986 1105 20-21 0.4424557571390224 1105 22-23 0.5370286113491431 1105 24-25 0.47974169911823594 1105 26-27 0.44418755751215855 1105 28-29 0.4984952670733378 1105 30-31 0.5374921010005664 1105 32-33 0.38077362132136017 1105 34-35 0.4433494552492192 1105 36-37 0.5393029651191839 1105 38-39 0.44746444372547245 1105 40-41 0.5903222359236153 1105 42-43 0.42212887741154503 1105 44-45 0.43079175018795013 1105 46-47 0.4120057826386869 1105 48-49 0.5587053858037478 1105 50-51 0.5689624248854983 1105 52-53 0.4338011203926371 1105 54-55 0.5043426255913417 1105 56-57 0.4630268792981411 1105 58-59 0.4728375880943503 1105 60-61 0.42434534888480613 1105 62-63 0.48580078560657114 1105 64-65 0.5161677595330829 1105 66-67 0.6619003701910273 1105 68-69 0.709578444991628 1105 70-71 0.6250658790963186 1105 72-73 0.5244141209269912 1105 74-75 0.5913284240280845 1105 76-77 0.6620460315106165 1105 78-79 0.6558469757122118 1105 80-81 0.7042202771691777 1105 82-83 0.6439107143203096 1105 84-85 0.580918447460018 1105 86-87 0.5546233824841806 1105 88-89 0.5523099877921531 1105 90-91 0.5081323551303711 1105 92-93 0.4989755024118203 1105 94-95 0.3575697850900106 1105 96-97 0.28854085302727484 1105 98-99 0.6484962497613189 1105 100-101 1.007227304713652 1106 1 0.13467675339088458 1106 2 0.18856244092830465 1106 3 0.2235315393309527 1106 4 0.09820455577362708 1106 5 0.15279919149515564 1106 6 0.0880927988276099 1106 7 0.1479232970697879 1106 8 0.12915195565612692 1106 9 0.14279294638966178 1106 10-11 0.19908023303061384 1106 12-13 0.3045682955959208 1106 14-15 0.279331253999473 1106 16-17 0.1939362049435971 1106 18-19 0.26016891090064576 1106 20-21 0.266662154094071 1106 22-23 0.22176027744681193 1106 24-25 0.3231676197872204 1106 26-27 0.2828158447174829 1106 28-29 0.3309144535109638 1106 30-31 0.3955521026300204 1106 32-33 0.27205916420654574 1106 34-35 0.23705332466504103 1106 36-37 0.32544793580026266 1106 38-39 0.3803599975545282 1106 40-41 0.3292668213181784 1106 42-43 0.4235928276556109 1106 44-45 0.2560301861330885 1106 46-47 0.3049942726856827 1106 48-49 0.44908554149962754 1106 50-51 0.20092614674970832 1106 52-53 -1.1706013452084107E-4 1106 54-55 0.11621283595832921 1106 56-57 0.16087047049865788 1106 58-59 0.38997493210820267 1106 60-61 0.15643236424401863 1106 62-63 0.17050715146341133 1106 64-65 0.2563848115038425 1106 66-67 0.18925806273678347 1106 68-69 0.2106359348192015 1106 70-71 0.2648880364749644 1106 72-73 0.23321429766723867 1106 74-75 0.3967216070890309 1106 76-77 0.38492134966491065 1106 78-79 0.3138226582652699 1106 80-81 0.28329670693774034 1106 82-83 0.3376034847614342 1106 84-85 0.38222104843019267 1106 86-87 0.4919336777394996 1106 88-89 0.39498375338511593 1106 90-91 0.2527901264532417 1106 92-93 0.46989746878870164 1106 94-95 0.46765075640239573 1106 96-97 0.39412026393224764 1106 98-99 0.3948706725430995 1106 100-101 0.6635356562177321 1107 1 0.11412050128309659 1107 2 0.1563288120136157 1107 3 0.21595531487702146 1107 4 0.04608916633196003 1107 5 0.149910995379976 1107 6 0.1987594629202576 1107 7 0.17783867674009457 1107 8 0.019648648464411167 1107 9 0.08903454178945935 1107 10-11 0.08306738412935033 1107 12-13 0.02680525241820675 1107 14-15 0.10445735734340644 1107 16-17 0.15952592249616515 1107 18-19 0.14265271203937857 1107 20-21 0.10958380154644232 1107 22-23 0.20589687996054096 1107 24-25 0.13577152408664261 1107 26-27 0.10353592313789761 1107 28-29 0.24440184971061996 1107 30-31 0.3031479440671774 1107 32-33 0.3558158862903227 1107 34-35 0.19593191038894986 1107 36-37 0.19462151729264576 1107 38-39 0.3023734306587542 1107 40-41 0.20726062021307 1107 42-43 0.34378734615668805 1107 44-45 0.20321483718764455 1107 46-47 0.25969218376653913 1107 48-49 0.19793514131168166 1107 50-51 0.14572238183333752 1107 52-53 0.17773079196916086 1107 54-55 0.2602310445579903 1107 56-57 0.2553429511085099 1107 58-59 0.3132311701529389 1107 60-61 0.2026481798687243 1107 62-63 0.24791359916930844 1107 64-65 0.18602785517457932 1107 66-67 0.1469412153419114 1107 68-69 0.14751587813933753 1107 70-71 0.15608231277088436 1107 72-73 0.08895462642768592 1107 74-75 0.417647775209101 1107 76-77 0.4463759675339496 1107 78-79 0.41216990144057064 1107 80-81 0.3485818340716804 1107 82-83 0.28106514128127813 1107 84-85 0.4157023085681679 1107 86-87 0.34186932482744226 1107 88-89 0.22543354363486756 1107 90-91 0.4360886338250509 1107 92-93 0.3690147193935083 1107 94-95 0.2391036947250278 1107 96-97 0.1955584516496991 1107 98-99 0.1978499294767211 1107 100-101 0.5428145828405597 1108 1 0.2152243441211148 1108 2 0.2662569451295589 1108 3 0.3181027480361607 1108 4 0.16342534191920066 1108 5 0.2224698722754468 1108 6 0.16695243706455187 1108 7 0.21447725543788465 1108 8 0.1749818475713525 1108 9 0.3009484986860542 1108 10-11 0.197440970047289 1108 12-13 0.29618598081348324 1108 14-15 0.4211400455436518 1108 16-17 0.45282842358799513 1108 18-19 0.479046516754309 1108 20-21 0.4191465660287079 1108 22-23 0.48552382218563395 1108 24-25 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-1.0585502950203711 2316 70-71 -0.8024207415134939 2316 72-73 -0.8459219725861331 2316 74-75 -0.5369574122944485 2316 76-77 -1.1955633956730445 2316 78-79 -0.9464561832148526 2316 80-81 -0.7838050105456702 2316 82-83 -0.927215110958695 2316 84-85 -0.9901735693047904 2316 86-87 -0.8716643969936833 2316 88-89 -1.1495360624601254 2316 90-91 -0.8233553845851667 2316 92-93 -0.9956167159746734 2316 94-95 -0.6698720150895525 2316 96-97 -0.85138714274078 2316 98-99 -0.9267572877508528 2316 100-101 -0.9661213118243843 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 23.0 4 37.0 5 93.0 6 165.0 7 516.0 8 1133.0 9 2064.0 10 3316.0 11 5042.0 12 7028.0 13 8804.0 14 10347.0 15 12590.0 16 14638.0 17 16777.0 18 15354.0 19 13885.0 20 14195.0 21 15059.0 22 16655.0 23 19768.0 24 24096.0 25 29665.0 26 38370.0 27 48481.0 28 61183.0 29 76044.0 30 91287.0 31 109868.0 32 131409.0 33 157758.0 34 193242.0 35 238556.0 36 306573.0 37 389853.0 38 327358.0 39 57239.0 40 11.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.471468330442804 21.293169347886494 14.605843802112512 23.6295185195582 2 15.635315360462803 21.929223331024254 39.44660957554256 22.98885173297039 3 18.403276914753842 26.440544905040248 30.886101179217462 24.270077000988444 4 13.023227805172393 18.088286573509148 38.57100599299915 30.31747962831931 5 12.805492903034235 38.09599157190826 35.75430810939435 13.344207415663153 6 30.22846599774495 38.68607646012746 17.825538014483723 13.259919527643865 7 26.077157273686847 32.80353551069053 23.390339985804264 17.728967229818362 8 24.79478215242314 36.4959314374503 20.89725989786393 17.812026512262634 9 25.314248409081202 16.08722444920347 21.049020026561074 37.54950711515425 10-11 24.002403919487 26.730079703557273 29.48718418050141 19.78033219645432 12-13 24.54572633146023 24.299070362438655 29.403799494404076 21.75140381169704 14-15 21.813906087266364 26.05194378894803 26.712472663545366 25.421677460240243 16-17 21.61933060401019 28.937943489588104 27.48965318071902 21.953072725682688 18-19 21.58188884617966 28.029335139323607 29.218807517637895 21.169968496858836 20-21 22.137271531044526 27.292946672442582 29.202150918146746 21.367630878366146 22-23 21.912512327213097 27.097212570269434 29.11278436483304 21.87749073768443 24-25 21.836174491061637 27.248898758600603 29.099395133284833 21.815531617052926 26-27 21.819664646082536 27.374104999191562 28.957217919032235 21.849012435693666 28-29 21.832288820441065 27.5358681625759 28.962500241510767 21.669342775472266 30-31 22.10465704933744 27.26107475250737 28.89933736876885 21.73493082938634 32-33 21.917379076757207 27.422131585237473 28.802083068457478 21.858406269547835 34-35 22.077089963513078 27.447530471197545 28.84165310289361 21.63372646239577 36-37 22.136052808572177 27.231957183030303 28.84473214886772 21.7872578595298 38-39 22.004403158478112 27.40592951895748 28.76871215241233 21.820955170152075 40-41 22.133127184962234 27.31474052375435 28.818041317942168 21.73409097334125 42-43 21.998782470421617 27.404015813646787 28.62988463571641 21.967317080215185 44-45 22.032598640828358 27.472661579603802 28.586843062293603 21.907896717274237 46-47 22.175011995216963 27.444303853337598 28.323905638575724 22.056778512869712 48-49 22.11422211446619 27.543763231075392 28.23495216879801 22.107062485660403 50-51 22.132462314015232 27.65321966069369 28.134956374320957 22.079361650970117 52-53 22.27571664518954 27.637754243487322 28.065078844756496 22.021450266566635 54-55 22.08036015958363 27.608037602600305 28.220318658726136 22.091283579089932 56-57 22.101865289505827 27.746733124576036 28.01392076918148 22.137480816736655 58-59 22.106977672351665 27.66990044392477 28.12098562162182 22.102136262101745 60-61 22.10576716069008 27.726926616014918 28.162000121277682 22.005306102017318 62-63 22.137907448272493 27.71464574494939 28.083089726155674 22.06435708062244 64-65 21.940270370849916 27.614458920356967 28.097127319898668 22.348143388894453 66-67 21.91466680999346 27.680292484382672 28.06348806926593 22.341552636357935 68-69 22.003947511734125 27.61920660918107 28.102965112668986 22.273880766415814 70-71 21.962985897625366 27.480169998774556 28.08939062873409 22.467453474865984 72-73 21.98796682583768 27.645386269042366 27.93322103239123 22.43342587272872 74-75 22.007593781612727 27.796037810704284 27.769126857824133 22.42724154985886 76-77 21.949936483356357 27.68454705738853 27.839524404342793 22.525992054912322 78-79 21.944402353264213 27.735222182108483 27.840295327540005 22.480080137087302 80-81 22.028220918786555 27.72847636325179 27.675292662886523 22.568010055075128 82-83 22.013862195620472 27.720811800763478 27.817538036636385 22.447787966979664 84-85 22.063242199972745 27.749426984504684 27.658944431224917 22.528386384297647 86-87 21.98329458996089 27.838628260900517 27.6595342253461 22.5185429237925 88-89 21.913445352344528 27.710417995823843 27.61666093480874 22.759475717022887 90-91 21.96584179640943 27.70903858967444 27.687031744365882 22.638087869550255 92-93 21.954794656529543 27.592667780838948 27.75533883259263 22.697198730038874 94-95 21.774459419360497 27.555940351714263 27.812159273882326 22.857440955042915 96-97 21.830802303044354 27.401102711629317 27.792319253524017 22.975775731802308 98-99 21.72020573645965 27.507163964799457 27.63022755884213 23.14240273989876 100-101 21.69632924341617 27.43110086089604 27.64668078105012 23.225889114637674 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 354.0 1 256.5 2 272.0 3 386.5 4 321.0 5 281.0 6 414.5 7 739.0 8 1143.5 9 1374.5 10 1256.0 11 1080.5 12 1140.5 13 1280.0 14 1457.5 15 1633.5 16 1825.5 17 2078.5 18 2316.0 19 2623.0 20 3108.5 21 3704.0 22 4524.0 23 5784.5 24 7575.5 25 10127.5 26 13675.0 27 18128.5 28 23464.0 29 30153.5 30 37795.5 31 45488.5 32 52750.0 33 57764.0 34 61002.0 35 64087.0 36 68933.5 37 73848.0 38 76197.0 39 77281.0 40 78321.5 41 80171.0 42 81365.0 43 82822.5 44 85068.5 45 87463.5 46 89141.5 47 89738.5 48 90350.5 49 89362.5 50 86923.5 51 84174.0 52 82196.0 53 79783.5 54 76035.0 55 70515.5 56 63374.0 57 56165.5 58 49305.5 59 42451.5 60 35248.5 61 28994.0 62 23389.5 63 17751.0 64 12893.0 65 9539.5 66 7198.0 67 5574.0 68 4184.0 69 3096.0 70 2379.0 71 1745.5 72 1265.5 73 939.5 74 698.5 75 524.5 76 355.5 77 206.0 78 128.0 79 81.5 80 44.5 81 25.0 82 18.0 83 11.0 84 6.5 85 4.5 86 2.5 87 2.0 88 1.0 89 0.0 90 0.5 91 0.5 92 0.5 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.018792060964374403 2 0.0030913347040962217 3 0.003050659247463377 4 0.031889558000150496 5 0.0016270182653138011 6 4.067545663284503E-5 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 2.0337728316422514E-5 16-17 0.0 18-19 0.0 20-21 0.0 22-23 6.101318494926754E-5 24-25 3.864168380120277E-4 26-27 0.0015253296237316884 28-29 1.0168864158211257E-4 30-31 0.007301244465595682 32-33 0.023286698922303777 34-35 0.013992357081698688 36-37 8.338468609733231E-4 38-39 9.151977742390132E-4 40-41 0.002501540582919969 42-43 0.009294341840605088 44-45 0.00907062682912444 46-47 0.008684209991112413 48-49 0.010453592354641172 50-51 0.1893442506258936 52-53 0.04960371936375451 54-55 0.01885307414932367 56-57 0.011694193781942945 58-59 0.02129360154729437 60-61 0.05344755001555836 62-63 0.09538394580402158 64-65 0.09969554420710317 66-67 0.10408849352345041 68-69 0.1019937075068589 70-71 0.09078761920451009 72-73 0.06888388580772305 74-75 0.2421003178786936 76-77 0.29119559403453754 78-79 0.008033402684986892 80-81 0.0010168864158211256 82-83 0.0 84-85 0.0 86-87 0.0 88-89 1.423640982149576E-4 90-91 0.006467397604622359 92-93 0.018588683681210177 94-95 0.0016473559936302235 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2458485.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 65.59620807741376 #Duplication Level Percentage of deduplicated Percentage of total 1 81.30739886503025 53.33457054183799 2 11.149512162674139 14.627314395688565 3 3.23605844882989 6.368194900803548 4 1.403424408409741 3.6823727805986666 5 0.7698866629926803 2.525082287084679 6 0.4781691914008281 1.8819651465202443 7 0.31917900109456543 1.465585251881813 8 0.23906645919302555 1.2545482561244998 9 0.181168591382869 1.0695575355698363 >10 0.8651949791257293 9.762347605240326 >50 0.03624542362480468 1.6112423976943573 >100 0.013515769519105032 1.5813570295343846 >500 9.937151346569371E-4 0.4138365691837419 >1k 1.8632158774560973E-4 0.422025302237264 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4318 0.17563662174062483 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3706 0.15074324228132366 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 4.067545663284503E-5 2 0.0 0.0 0.0 0.0 4.067545663284503E-5 3 0.0 0.0 0.0 0.0 4.067545663284503E-5 4 0.0 0.0 0.0 0.0 4.067545663284503E-5 5 0.0 0.0 0.0 0.0 4.067545663284503E-5 6 0.0 0.0 0.0 0.0 4.067545663284503E-5 7 0.0 0.0 0.0 0.0 4.067545663284503E-5 8 0.0 0.0 0.0 0.0 4.067545663284503E-5 9 0.0 0.0 0.0 1.2202636989853507E-4 8.135091326569005E-5 10-11 0.0 0.0 0.0 1.2202636989853507E-4 8.135091326569005E-5 12-13 0.0 0.0 0.0 1.2202636989853507E-4 1.627018265313801E-4 14-15 0.0 0.0 0.0 1.2202636989853507E-4 1.627018265313801E-4 16-17 4.067545663284503E-5 0.0 0.0 1.627018265313801E-4 1.627018265313801E-4 18-19 4.067545663284503E-5 0.0 0.0 1.627018265313801E-4 2.4405273979707015E-4 20-21 4.067545663284503E-5 0.0 0.0 1.627018265313801E-4 2.847281964299152E-4 22-23 4.067545663284503E-5 0.0 0.0 2.2371501148064763E-4 2.847281964299152E-4 24-25 4.067545663284503E-5 2.0337728316422514E-5 0.0 4.677677512777178E-4 3.254036530627602E-4 26-27 8.135091326569005E-5 4.067545663284503E-5 0.0 7.93171404340478E-4 3.864168380120277E-4 28-29 8.135091326569005E-5 4.067545663284503E-5 0.0 0.001749044635212336 4.0675456632845023E-4 30-31 8.135091326569005E-5 4.067545663284503E-5 0.0 0.005328484818902699 4.2709229464487275E-4 32-33 8.135091326569005E-5 4.067545663284503E-5 0.0 0.016066805369973784 4.881054795941403E-4 34-35 8.135091326569005E-5 4.067545663284503E-5 0.0 0.03353691399378072 6.914827627583655E-4 36-37 8.135091326569005E-5 4.067545663284503E-5 0.0 0.0612572376890646 6.914827627583655E-4 38-39 8.135091326569005E-5 4.067545663284503E-5 0.0 0.10124121155915126 7.524959477076329E-4 40-41 8.135091326569005E-5 4.067545663284503E-5 0.0 0.14388942783868927 7.728336760240554E-4 42-43 8.135091326569005E-5 4.067545663284503E-5 0.0 0.1852767049626091 7.931714043404779E-4 44-45 8.135091326569005E-5 4.067545663284503E-5 0.0 0.2374226403659164 8.33846860973323E-4 46-47 8.135091326569005E-5 4.067545663284503E-5 0.0 0.3011610809095846 8.948600459225905E-4 48-49 8.135091326569005E-5 4.067545663284503E-5 0.0 0.36835693526704455 9.762109591882806E-4 50-51 8.135091326569005E-5 4.067545663284503E-5 0.0 0.4424472795237718 9.762109591882806E-4 52-53 8.135091326569005E-5 4.067545663284503E-5 0.0 0.5118802839960382 0.0010168864158211256 54-55 8.135091326569005E-5 4.067545663284503E-5 0.0 0.5911567489734532 0.0010575618724539707 56-57 8.135091326569005E-5 4.067545663284503E-5 0.0 0.6829612545937844 0.0010982373290868157 58-59 8.135091326569005E-5 4.067545663284503E-5 0.0 0.785565093950136 0.0011592505140360834 60-61 1.2202636989853507E-4 4.067545663284503E-5 0.0 0.8969344942108657 0.0012609391556181958 62-63 1.2202636989853507E-4 4.067545663284503E-5 0.0 1.0112528650774766 0.001382965525516731 64-65 1.2202636989853507E-4 4.067545663284503E-5 0.0 1.1318352562655456 0.0014033032538331533 66-67 1.2202636989853507E-4 4.067545663284503E-5 0.0 1.2688708696616005 0.0014439787104659984 68-69 1.2202636989853507E-4 4.067545663284503E-5 0.0 1.426000158634281 0.0014643164387824208 70-71 1.2202636989853507E-4 4.067545663284503E-5 0.0 1.5956778259781939 0.0014846541670988432 72-73 1.2202636989853507E-4 4.067545663284503E-5 0.0 1.7724126850479056 0.0016473559936302233 74-75 1.2202636989853507E-4 4.067545663284503E-5 0.0 1.959723162842157 0.0017287069068959134 76-77 1.2202636989853507E-4 4.067545663284503E-5 0.0 2.17379809110082 0.001749044635212336 78-79 1.2202636989853507E-4 4.067545663284503E-5 0.0 2.4096954018430052 0.001789720091845181 80-81 1.2202636989853507E-4 4.067545663284503E-5 0.0 2.670933522067452 0.001789720091845181 82-83 1.2202636989853507E-4 4.067545663284503E-5 0.0 2.9505162732333123 0.001830395548478026 84-85 1.627018265313801E-4 4.067545663284503E-5 0.0 3.238864585303551 0.001830395548478026 86-87 1.627018265313801E-4 4.067545663284503E-5 0.0 3.544357602344533 0.0018914087334272935 88-89 1.627018265313801E-4 4.067545663284503E-5 0.0 3.8854416439392554 0.0019930973750094064 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2650 0.0 48.02653 1 GTATCAA 5055 0.0 43.120502 1 TCAACGC 6325 0.0 33.701214 4 ATCAACG 6300 0.0 33.68492 3 CAACGCA 6370 0.0 33.378426 5 AACGCAG 6795 0.0 31.639965 6 TATCAAC 6785 0.0 31.556969 2 ACGCAGA 7710 0.0 27.700357 7 CGCAGAG 7930 0.0 26.752329 8 GTACATG 6045 0.0 23.960499 1 GTGGTAT 1665 0.0 23.102716 1 GCAGAGT 9120 0.0 22.689184 9 TACATGG 6405 0.0 22.236717 2 TATCACG 300 7.2759576E-12 22.155184 2 ACATGGG 6120 0.0 22.108639 3 AGAGTAC 8380 0.0 21.209724 10-11 TGGTATC 1610 0.0 20.641474 2 TAACGCA 300 1.6916601E-10 20.572252 4 ATAACGC 220 1.3527224E-6 19.421751 3 ATCACGC 345 5.2750693E-11 19.265377 3 >>END_MODULE