FastQCFastQC Report
Sat 18 Jun 2016
SRR1930908_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1930908_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences873322
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN16030.18355200029313357No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA7300.062.9882771
GTATCAA15400.048.8885041
TCAACGC18850.038.5673984
ATCAACG19100.038.083343
TATCAAC20000.037.388592
CAACGCA20250.035.139125
AACGCAG21600.032.2379656
GTACATG13150.030.355861
GTGGTAT3650.029.0715141
TACATGG13450.028.5495132
ACGCAGA25900.026.3729977
CGCAGAG26550.025.9383288
ACATGGG14400.025.256663
GCAGAGT27200.023.4118239
CATGGGG9700.021.8598294
TGGTATC3950.020.4658722
AGAGTAC24550.020.2201410-11
GTACTAC2007.5251937E-620.2116241
GAGTACT20150.020.12256212-13
CAGAGTA25900.019.76247610-11
Produced by FastQC (version 0.11.5)