##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1930908_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 873322 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 22.74579937296896 30.0 10.0 31.0 2.0 33.0 2 21.575248304748992 28.0 10.0 31.0 2.0 33.0 3 22.036021078136127 28.0 10.0 31.0 2.0 33.0 4 23.940699993816715 32.0 2.0 35.0 2.0 37.0 5 23.924923453205118 32.0 10.0 35.0 2.0 37.0 6 24.73706490847591 32.0 11.0 35.0 2.0 37.0 7 24.828643959501765 32.0 11.0 35.0 2.0 37.0 8 25.692854411087776 33.0 17.0 35.0 2.0 37.0 9 26.893603962799517 33.0 17.0 37.0 2.0 39.0 10-11 27.18794728633883 33.5 17.5 37.0 2.0 39.0 12-13 27.166817622824112 34.0 17.5 37.0 2.0 39.0 14-15 27.37830662687989 34.0 17.0 38.0 2.0 40.0 16-17 26.470915653103894 33.0 13.5 38.0 2.0 40.0 18-19 25.848973230950328 32.5 10.0 38.0 2.0 40.0 20-21 25.35078928505179 32.0 10.0 38.0 2.0 40.0 22-23 25.29581128152045 32.0 10.0 38.0 2.0 40.0 24-25 25.023997448821852 32.0 9.5 38.0 2.0 40.0 26-27 24.728740372966673 31.5 8.5 38.0 2.0 40.0 28-29 24.659566002001554 31.5 5.5 38.0 2.0 40.0 30-31 24.578355406138858 31.0 8.0 38.0 2.0 40.0 32-33 24.297307293300754 31.0 4.0 37.0 2.0 39.5 34-35 21.38367978821099 25.5 2.0 36.0 2.0 39.0 36-37 19.707000968714862 19.0 2.0 35.0 2.0 38.5 38-39 18.803527221345618 18.5 2.0 34.0 2.0 38.0 40-41 20.20823590840492 24.0 2.0 35.0 2.0 38.0 42-43 20.142393069223033 24.0 2.0 35.0 2.0 38.0 44-45 18.114975919534835 18.5 2.0 34.0 2.0 38.0 46-47 19.00970203430121 19.0 2.0 33.0 2.0 38.0 48-49 18.46641273207362 18.5 2.0 34.0 2.0 38.0 50-51 16.436155278350938 11.5 2.0 32.0 2.0 37.5 52-53 19.466958349841182 23.0 2.0 33.5 2.0 38.0 54-55 21.143585641951077 27.5 2.0 35.5 2.0 38.5 56-57 21.38453170766338 28.0 2.0 36.0 2.0 39.0 58-59 21.02116745026462 27.5 2.0 36.0 2.0 39.0 60-61 20.73451487538388 27.0 2.0 36.0 2.0 39.0 62-63 20.276810271583678 26.0 2.0 35.0 2.0 39.0 64-65 19.786909639285398 25.5 2.0 35.0 2.0 38.0 66-67 19.38183167262476 25.0 2.0 34.0 2.0 38.0 68-69 18.940901523149535 23.5 2.0 34.0 2.0 38.0 70-71 18.358575073111638 22.0 2.0 34.0 2.0 37.0 72-73 17.890798582882375 20.5 2.0 33.0 2.0 36.5 74-75 17.347320346905263 18.0 2.0 32.5 2.0 36.0 76-77 16.780491617066787 15.0 2.0 32.0 2.0 35.5 78-79 16.281141434659837 9.5 2.0 31.5 2.0 35.0 80-81 15.761014265070614 7.0 2.0 31.0 2.0 35.0 82-83 15.27428714723779 2.0 2.0 31.0 2.0 34.0 84-85 14.749228806786043 2.0 2.0 31.0 2.0 34.0 86-87 14.15831789420168 2.0 2.0 30.0 2.0 34.0 88-89 13.598612539246691 2.0 2.0 29.5 2.0 34.0 90-91 13.01100567717291 2.0 2.0 29.0 2.0 34.0 92-93 12.369100973066063 2.0 2.0 29.0 2.0 34.0 94-95 11.69259791920964 2.0 2.0 27.5 2.0 34.0 96-97 10.945416467236598 2.0 2.0 26.0 2.0 33.5 98-99 10.130980325698882 2.0 2.0 25.0 2.0 33.0 100-101 9.054122648919872 2.0 2.0 17.5 2.0 31.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 6.765724102451657 1101 2 7.81445269674991 1101 3 7.266411394607697 1101 4 9.188715965423931 1101 5 9.204831096929635 1101 6 8.52516946427567 1101 7 8.530966102610478 1101 8 7.083581541291625 1101 9 7.237378493468345 1101 10-11 6.818681692874559 1101 12-13 6.650061187698672 1101 14-15 7.404289984929516 1101 16-17 8.145479476541851 1101 18-19 8.724915838776152 1101 20-21 9.161848484370342 1101 22-23 8.8622464437221 1101 24-25 8.83458479143108 1101 26-27 8.771173577345124 1101 28-29 8.667627617647938 1101 30-31 8.220891177735037 1101 32-33 8.239833189856881 1101 34-35 10.981912879076873 1101 36-37 4.910767055656283 1101 38-39 8.341031660405637 1101 40-41 9.730219539398465 1101 42-43 10.315643261719895 1101 44-45 4.47651662714545 1101 46-47 6.052014332362386 1101 48-49 9.167489087647223 1101 50-51 -3.3789128124305527 1101 52-53 5.953080252528377 1101 54-55 8.56041941272845 1101 56-57 8.756510963204612 1101 58-59 9.11392160485152 1101 60-61 8.722268897079584 1101 62-63 9.034817410923878 1101 64-65 9.255362734868015 1101 66-67 8.738898671475415 1101 68-69 8.851407186038703 1101 70-71 8.822993438499271 1101 72-73 8.693241591973923 1101 74-75 8.699296079707466 1101 76-77 8.657499435462995 1101 78-79 8.457900552567938 1101 80-81 8.310587815080579 1101 82-83 8.206393244210306 1101 84-85 8.114194137060176 1101 86-87 7.798422349088996 1101 88-89 7.514586498492738 1101 90-91 7.577777909928379 1101 92-93 7.337759770050457 1101 94-95 7.040534888674577 1101 96-97 6.406008183183021 1101 98-99 6.185547577972585 1101 100-101 5.934981814441761 1102 1 6.5936456770479595 1102 2 7.631416869215897 1102 3 7.330867696065209 1102 4 9.419575392172113 1102 5 9.230663789025929 1102 6 8.296574802186448 1102 7 8.100042129364859 1102 8 7.110107614976098 1102 9 7.254906108940343 1102 10-11 6.918935932790223 1102 12-13 6.525589327434798 1102 14-15 6.847045838507615 1102 16-17 7.793791261492089 1102 18-19 8.002830789605245 1102 20-21 8.3625119885405 1102 22-23 8.33782447310908 1102 24-25 8.38515091213021 1102 26-27 8.476687934008211 1102 28-29 8.08657877934396 1102 30-31 7.9338178117620295 1102 32-33 8.08664307082637 1102 34-35 10.555323950069564 1102 36-37 1.1648639261376736 1102 38-39 4.00767295945322 1102 40-41 8.871951950477914 1102 42-43 9.962700758837851 1102 44-45 0.9732632299984623 1102 46-47 2.2208612049577496 1102 48-49 8.109209801435654 1102 50-51 -4.715419916773207 1102 52-53 5.332006243909024 1102 54-55 7.7402011384593905 1102 56-57 8.276646510849226 1102 58-59 8.240140307551478 1102 60-61 8.014204390042888 1102 62-63 8.207782293683199 1102 64-65 8.25126163645011 1102 66-67 7.972270197168587 1102 68-69 8.136907759628759 1102 70-71 7.9947836595639785 1102 72-73 8.086751850357217 1102 74-75 7.99561101024247 1102 76-77 7.78210518038313 1102 78-79 7.474949001901759 1102 80-81 7.250193855956267 1102 82-83 6.9418167284246035 1102 84-85 6.81496404429925 1102 86-87 7.009740957928772 1102 88-89 6.933614467702084 1102 90-91 6.995545249400674 1102 92-93 6.5961613845584814 1102 94-95 6.081781932642295 1102 96-97 5.973612183374689 1102 98-99 5.968681211771273 1102 100-101 5.619880752665972 1103 1 6.3427390286470064 1103 2 7.452880143982135 1103 3 7.141915797546112 1103 4 8.993108161604653 1103 5 8.898503921558547 1103 6 8.210383468842501 1103 7 7.822193554249772 1103 8 6.871469260164627 1103 9 6.885588258984679 1103 10-11 6.4855354304831145 1103 12-13 6.233401636613852 1103 14-15 6.697170087834596 1103 16-17 7.691416984288651 1103 18-19 8.054991612836684 1103 20-21 8.08589263219449 1103 22-23 7.939726178085948 1103 24-25 8.07059303265206 1103 26-27 8.309581881366846 1103 28-29 8.221506114726878 1103 30-31 7.792084639846625 1103 32-33 7.807553329891533 1103 34-35 10.466273289264922 1103 36-37 -3.1697211926160094 1103 38-39 -1.6848192484148434 1103 40-41 6.904880677028508 1103 42-43 9.039610561260186 1103 44-45 0.9457332429012766 1103 46-47 0.48923975124062125 1103 48-49 7.118856381127788 1103 50-51 -5.255644308933817 1103 52-53 4.057883821153347 1103 54-55 6.815430389087275 1103 56-57 7.568807652668681 1103 58-59 7.797020215798128 1103 60-61 8.013198182886338 1103 62-63 7.794171417088265 1103 64-65 7.889154495299689 1103 66-67 7.608263474961408 1103 68-69 7.67688790097564 1103 70-71 7.6467753656559125 1103 72-73 7.47883281419217 1103 74-75 7.335516548895164 1103 76-77 7.209487024051988 1103 78-79 7.218739190452766 1103 80-81 6.829960330939143 1103 82-83 6.93889235591025 1103 84-85 6.594071042085064 1103 86-87 6.366902020101223 1103 88-89 6.097080652238885 1103 90-91 6.148325460836922 1103 92-93 6.240347684211752 1103 94-95 6.054712570721 1103 96-97 5.97233252150277 1103 98-99 5.767638897972695 1103 100-101 4.945666204875113 1104 1 5.926596621234857 1104 2 6.938598451678381 1104 3 6.524081598267877 1104 4 8.185304419698895 1104 5 8.22412650608415 1104 6 7.294235942544219 1104 7 6.837882940239378 1104 8 6.043996198302885 1104 9 5.89578508015639 1104 10-11 5.695743769148624 1104 12-13 5.250796891406399 1104 14-15 5.74418577722059 1104 16-17 6.572313301250077 1104 18-19 6.990443738454143 1104 20-21 7.162742469669855 1104 22-23 7.08630932159393 1104 24-25 7.244599328882 1104 26-27 7.34775469496261 1104 28-29 7.1413494768112855 1104 30-31 6.796282126473539 1104 32-33 6.9573562527755115 1104 34-35 9.439258367711851 1104 36-37 -3.7512317759130838 1104 38-39 -2.241330471572681 1104 40-41 5.697860124700696 1104 42-43 7.894097264953462 1104 44-45 0.16834848182528717 1104 46-47 -0.4652981413139585 1104 48-49 6.48522273980814 1104 50-51 -5.629585339365596 1104 52-53 3.31123413212568 1104 54-55 5.9775967016976495 1104 56-57 6.142408277172791 1104 58-59 6.634640189691812 1104 60-61 6.634076839690618 1104 62-63 6.914590653862344 1104 64-65 6.733971623092682 1104 66-67 6.477487451824043 1104 68-69 6.38697978498168 1104 70-71 6.934916186213357 1104 72-73 6.677757848104786 1104 74-75 6.972239437994496 1104 76-77 6.800066993850564 1104 78-79 6.4259337849090095 1104 80-81 6.465425253771938 1104 82-83 6.144616550325546 1104 84-85 5.8756264156357805 1104 86-87 5.74978574460277 1104 88-89 5.382532777856353 1104 90-91 5.470293160665442 1104 92-93 5.218917467426927 1104 94-95 5.528830202724274 1104 96-97 4.9427159260739355 1104 98-99 4.347979815788978 1104 100-101 3.98983963273721 1105 1 5.343634099221813 1105 2 6.47974734668545 1105 3 6.368881558582384 1105 4 8.154396862092916 1105 5 7.736483576302671 1105 6 7.113768645870362 1105 7 6.969266723755016 1105 8 6.136312094822966 1105 9 6.243397749749864 1105 10-11 5.3820026673801316 1105 12-13 5.303075086708635 1105 14-15 5.668438573807638 1105 16-17 6.024560492261315 1105 18-19 6.863441481341763 1105 20-21 7.266825114707931 1105 22-23 7.110702257576264 1105 24-25 6.781799517388091 1105 26-27 6.893215666761105 1105 28-29 6.831191230910054 1105 30-31 6.786621189399849 1105 32-33 6.3122524504092645 1105 34-35 8.553174333681543 1105 36-37 -3.955707555360961 1105 38-39 -2.3956599107670655 1105 40-41 5.648538498587641 1105 42-43 7.519725411888995 1105 44-45 -3.4547844397710623 1105 46-47 -4.3831925779039835 1105 48-49 4.792131736013218 1105 50-51 -9.963941814301727 1105 52-53 0.46916875027683247 1105 54-55 5.233493717844684 1105 56-57 6.155280018621809 1105 58-59 6.65974002350881 1105 60-61 6.637581564744565 1105 62-63 6.412350903880949 1105 64-65 6.258069398224759 1105 66-67 6.393865158498645 1105 68-69 6.141704838929147 1105 70-71 6.060541852805613 1105 72-73 5.895794904433384 1105 74-75 5.977453119568025 1105 76-77 6.173362377931717 1105 78-79 5.751786402911343 1105 80-81 5.546092218079798 1105 82-83 5.559628828024779 1105 84-85 5.462573609127359 1105 86-87 5.741208717542223 1105 88-89 5.157738026262729 1105 90-91 5.246691454189261 1105 92-93 5.076436628078756 1105 94-95 4.313635619793997 1105 96-97 4.152423989669938 1105 98-99 4.030340854556744 1105 100-101 3.6881976454861753 1106 1 5.715503364007034 1106 2 6.735170106373619 1106 3 6.664641357480278 1106 4 7.941100469727072 1106 5 7.853541444987897 1106 6 7.132572493573683 1106 7 6.797100140723831 1106 8 6.25811009275446 1106 9 6.058608098874988 1106 10-11 5.776405215552995 1106 12-13 5.51541202696934 1106 14-15 5.620622048850098 1106 16-17 6.37781556163165 1106 18-19 6.807045251770614 1106 20-21 7.405475073125782 1106 22-23 6.887483281038968 1106 24-25 7.138279853626283 1106 26-27 7.0693608249735185 1106 28-29 6.97568389336903 1106 30-31 6.224351628709936 1106 32-33 6.472677083061026 1106 34-35 9.023054369774375 1106 36-37 -0.3907386889633422 1106 38-39 1.1473664214519133 1106 40-41 6.45284855298604 1106 42-43 7.840802024345738 1106 44-45 -3.7417866614121547 1106 46-47 -4.589316344280633 1106 48-49 4.5035857918628714 1106 50-51 -10.003522600286775 1106 52-53 0.1155078721278393 1106 54-55 4.82035328798187 1106 56-57 5.526053187417055 1106 58-59 6.198027404446048 1106 60-61 6.104610337706038 1106 62-63 6.023023615861554 1106 64-65 5.971940637278525 1106 66-67 5.862305721942636 1106 68-69 5.564821450349282 1106 70-71 5.489604829145023 1106 72-73 5.496491434856676 1106 74-75 5.275868639486227 1106 76-77 5.010523506987155 1106 78-79 4.616265707252612 1106 80-81 4.788228126914765 1106 82-83 4.602897820935789 1106 84-85 4.386758045388875 1106 86-87 4.448343177480828 1106 88-89 4.600735144961503 1106 90-91 4.0596136072257 1106 92-93 4.059691273381858 1106 94-95 3.945357968843732 1106 96-97 3.4393667241025465 1106 98-99 3.1181104022890445 1106 100-101 2.8235163202289666 1107 1 5.766640788487994 1107 2 6.881775581777635 1107 3 6.344410668571463 1107 4 7.783841076092223 1107 5 8.111480596408615 1107 6 6.475518248948472 1107 7 6.586614951790814 1107 8 6.198894637888394 1107 9 6.296280141675066 1107 10-11 5.4270491298882995 1107 12-13 5.267880153958888 1107 14-15 5.57824552435093 1107 16-17 6.586115567855273 1107 18-19 6.847334557975106 1107 20-21 7.347863929615986 1107 22-23 7.572378436064767 1107 24-25 7.34755028381684 1107 26-27 7.021671747890508 1107 28-29 7.114748413885085 1107 30-31 6.991807158628269 1107 32-33 6.636709275320225 1107 34-35 9.105395189125687 1107 36-37 -0.1412953573434237 1107 38-39 0.7296732413887099 1107 40-41 6.870330821426979 1107 42-43 7.651892057100426 1107 44-45 -3.5596552554038237 1107 46-47 -4.284685624023206 1107 48-49 4.787068300028494 1107 50-51 -9.964316088995513 1107 52-53 0.36774030826080306 1107 54-55 4.919439107114208 1107 56-57 6.2108359714965715 1107 58-59 6.525883182387226 1107 60-61 6.123163645330436 1107 62-63 5.918569346802865 1107 64-65 6.263397448801907 1107 66-67 6.24750468190631 1107 68-69 6.2669819690766175 1107 70-71 6.2523707449048445 1107 72-73 6.28409536357546 1107 74-75 6.424321608499504 1107 76-77 6.515604402543261 1107 78-79 6.353850158340709 1107 80-81 6.376132172499338 1107 82-83 5.843728351718516 1107 84-85 5.560957791372912 1107 86-87 5.376692580819915 1107 88-89 5.377544547701282 1107 90-91 5.702002578968155 1107 92-93 5.572106246032501 1107 94-95 5.4984045468891 1107 96-97 5.320134811214812 1107 98-99 5.117005709447824 1107 100-101 4.584713076569569 1108 1 6.157104681811312 1108 2 6.479524196671051 1108 3 6.738053264120342 1108 4 8.025924839825905 1108 5 8.182541259381662 1108 6 7.281253529256482 1108 7 7.412272290508064 1108 8 6.364318621361019 1108 9 5.08750466979977 1108 10-11 5.832871272812522 1108 12-13 5.3300714395256 1108 14-15 5.875652557875203 1108 16-17 6.1044517966091405 1108 18-19 6.744321364247991 1108 20-21 7.599357027559453 1108 22-23 7.7651136694200105 1108 24-25 7.277690437821914 1108 26-27 7.615732223052497 1108 28-29 7.73543228091485 1108 30-31 7.262275042936654 1108 32-33 7.503625182917183 1108 34-35 9.902521679875996 1108 36-37 -3.5261401784533852 1108 38-39 -1.8666480094329643 1108 40-41 5.986544785179447 1108 42-43 8.000997606917313 1108 44-45 -3.2689988557418843 1108 46-47 -4.033190178441462 1108 48-49 5.703890302094415 1108 50-51 -10.048662036971848 1108 52-53 0.759444928412929 1108 54-55 5.612330458414796 1108 56-57 6.593824984549439 1108 58-59 6.88934219262293 1108 60-61 6.998292765435611 1108 62-63 7.139938724209195 1108 64-65 7.194432052990091 1108 66-67 6.889114222708262 1108 68-69 6.811057703887396 1108 70-71 6.921023904825965 1108 72-73 7.061062684450658 1108 74-75 6.934311983182575 1108 76-77 6.997428987011283 1108 78-79 6.916468714073645 1108 80-81 6.634550803356694 1108 82-83 6.529741122870734 1108 84-85 6.626456899270368 1108 86-87 6.488024630777661 1108 88-89 6.225540838432805 1108 90-91 6.384118270582391 1108 92-93 6.229200808902252 1108 94-95 5.9537630312538266 1108 96-97 5.8929785054584 1108 98-99 6.093467114274567 1108 100-101 5.936536017690802 1109 1 4.986259066483381 1109 2 6.557849546707899 1109 3 6.03248952056839 1109 4 8.06440125839628 1109 5 7.834862556360292 1109 6 5.218283742963187 1109 7 4.588930359778512 1109 8 3.549985533667577 1109 9 2.6354965636833363 1109 10-11 2.688628457779359 1109 12-13 2.945689530903646 1109 14-15 3.81146224836894 1109 16-17 3.0881198142952115 1109 18-19 4.171292624380637 1109 20-21 4.856740041560926 1109 22-23 6.040122880915959 1109 24-25 5.607415935242695 1109 26-27 4.889672901018599 1109 28-29 4.905969311128558 1109 30-31 5.117875632472394 1109 32-33 4.869561070905391 1109 34-35 6.933711805152338 1109 36-37 -4.709439736301581 1109 38-39 -3.001951067649621 1109 40-41 3.379069656218501 1109 42-43 4.37060888178835 1109 44-45 -4.446002356110345 1109 46-47 -6.069778240342714 1109 48-49 2.4709361753442316 1109 50-51 -10.243664800420632 1109 52-53 -1.130781674387368 1109 54-55 2.337998291870953 1109 56-57 3.9025206367233523 1109 58-59 4.65655387279979 1109 60-61 4.997256472141579 1109 62-63 4.851324133199611 1109 64-65 4.520118309438139 1109 66-67 5.051697126171785 1109 68-69 5.141824100350181 1109 70-71 5.006096675643946 1109 72-73 4.769868874575938 1109 74-75 5.1149217842142605 1109 76-77 4.891837608971493 1109 78-79 5.017698448782561 1109 80-81 5.128531090755368 1109 82-83 4.9693689784344794 1109 84-85 4.632697687774419 1109 86-87 4.426717519502789 1109 88-89 4.819843528189621 1109 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-11.90363733044952 2313 88-89 -11.358510074422458 2313 90-91 -10.765152386296963 2313 92-93 -10.1591249957279 2313 94-95 -9.656563055702692 2313 96-97 -8.882781423018919 2313 98-99 -7.998851255761194 2313 100-101 -6.909631401846183 2314 1 -20.771156187753906 2314 2 -19.60634536854634 2314 3 -20.014120648923136 2314 4 -22.00233602973931 2314 5 -21.95115439279225 2314 6 -22.737224731613082 2314 7 -22.847510318187588 2314 8 -23.666116161247675 2314 9 -24.858147504113273 2314 10-11 -25.132346118491828 2314 12-13 -25.10145029960483 2314 14-15 -25.35749961603784 2314 16-17 -24.475439507738685 2314 18-19 -23.857309070534615 2314 20-21 -23.381077755049244 2314 22-23 -23.313690678406076 2314 24-25 -23.023939996960696 2314 26-27 -22.734267776947505 2314 28-29 -22.640111197346016 2314 30-31 -22.563268435324215 2314 32-33 -22.290396556213253 2314 34-35 -19.45400005925444 2314 36-37 -17.79842278714904 2314 38-39 -16.917734965954704 2314 40-41 -18.28247695395099 2314 42-43 -18.21095891482182 2314 44-45 -16.21030320356797 2314 46-47 -17.09732061322407 2314 48-49 -16.5423053500795 2314 50-51 -14.531270732624023 2314 52-53 -17.526968115065333 2314 54-55 -19.157234758976507 2314 56-57 -19.347479363276648 2314 58-59 -19.03783172042055 2314 60-61 -18.729968104129608 2314 62-63 -18.274735188834285 2314 64-65 -17.745241860053387 2314 66-67 -17.38262490772652 2314 68-69 -16.907964035243037 2314 70-71 -16.357780443000124 2314 72-73 -15.85089383728847 2314 74-75 -15.357535842904383 2314 76-77 -14.780831882553933 2314 78-79 -14.247661720708965 2314 80-81 -13.74805789229548 2314 82-83 -13.261563224955351 2314 84-85 -12.732238752903546 2314 86-87 -12.110888412700602 2314 88-89 -11.565220031132414 2314 90-91 -10.972403468548045 2314 92-93 -10.349060060662964 2314 94-95 -9.656563055702692 2314 96-97 -8.932564972802469 2314 98-99 -8.107076363986302 2314 100-101 -7.0281378953526765 2315 1 -20.771156187753906 2315 2 -19.60634536854634 2315 3 -20.014120648923136 2315 4 -16.64753138348799 2315 5 -21.95115439279225 2315 6 -22.737224731613082 2315 7 -22.847510318187588 2315 8 -23.666116161247675 2315 9 -19.916225645612744 2315 10-11 -18.096443267383066 2315 12-13 -14.55921165124158 2315 14-15 -14.481047662500352 2315 16-17 -20.04354932822443 2315 18-19 -23.857309070534615 2315 20-21 -23.381077755049244 2315 22-23 -23.313690678406076 2315 24-25 -23.023939996960696 2315 26-27 -22.734267776947505 2315 28-29 -22.640111197346016 2315 30-31 -22.563268435324215 2315 32-33 -22.290396556213253 2315 34-35 -19.45400005925444 2315 36-37 -17.79842278714904 2315 38-39 -16.917734965954704 2315 40-41 -18.28247695395099 2315 42-43 -18.21095891482182 2315 44-45 -16.21030320356797 2315 46-47 -17.09732061322407 2315 48-49 -16.5423053500795 2315 50-51 -14.531270732624023 2315 52-53 -17.526968115065333 2315 54-55 -19.157234758976507 2315 56-57 -19.347479363276648 2315 58-59 -19.03783172042055 2315 60-61 -18.729968104129608 2315 62-63 -18.274735188834285 2315 64-65 -17.745241860053387 2315 66-67 -17.38262490772652 2315 68-69 -16.907964035243037 2315 70-71 -16.357780443000124 2315 72-73 -15.85089383728847 2315 74-75 -15.357535842904383 2315 76-77 -14.780831882553933 2315 78-79 -14.247661720708965 2315 80-81 -13.74805789229548 2315 82-83 -13.261563224955351 2315 84-85 -12.732238752903546 2315 86-87 -12.110888412700602 2315 88-89 -11.565220031132414 2315 90-91 -10.972403468548045 2315 92-93 -10.349060060662964 2315 94-95 -9.656563055702692 2315 96-97 -8.932564972802469 2315 98-99 -8.107076363986302 2315 100-101 -7.0281378953526765 2316 1 -20.771156187753906 2316 2 -19.60634536854634 2316 3 -20.014120648923136 2316 4 -22.00233602973931 2316 5 -21.95115439279225 2316 6 -22.737224731613082 2316 7 -22.847510318187588 2316 8 -23.666116161247675 2316 9 -24.858147504113273 2316 10-11 -25.132346118491828 2316 12-13 -25.10145029960483 2316 14-15 -25.35749961603784 2316 16-17 -24.475439507738685 2316 18-19 -23.857309070534615 2316 20-21 -23.381077755049244 2316 22-23 -23.313690678406076 2316 24-25 -23.023939996960696 2316 26-27 -22.734267776947505 2316 28-29 -22.640111197346016 2316 30-31 -22.563268435324215 2316 32-33 -22.290396556213253 2316 34-35 -19.45400005925444 2316 36-37 -17.79842278714904 2316 38-39 -16.917734965954704 2316 40-41 -18.28247695395099 2316 42-43 -18.21095891482182 2316 44-45 -16.21030320356797 2316 46-47 -17.09732061322407 2316 48-49 -16.5423053500795 2316 50-51 -14.531270732624023 2316 52-53 -17.526968115065333 2316 54-55 -19.157234758976507 2316 56-57 -19.347479363276648 2316 58-59 -19.03783172042055 2316 60-61 -18.729968104129608 2316 62-63 -18.274735188834285 2316 64-65 -17.745241860053387 2316 66-67 -17.38262490772652 2316 68-69 -16.907964035243037 2316 70-71 -16.357780443000124 2316 72-73 -15.85089383728847 2316 74-75 -15.357535842904383 2316 76-77 -14.780831882553933 2316 78-79 -14.247661720708965 2316 80-81 -13.74805789229548 2316 82-83 -13.261563224955351 2316 84-85 -12.732238752903546 2316 86-87 -12.110888412700602 2316 88-89 -11.565220031132414 2316 90-91 -10.972403468548045 2316 92-93 -10.349060060662964 2316 94-95 -9.656563055702692 2316 96-97 -8.932564972802469 2316 98-99 -8.107076363986302 2316 100-101 -7.0281378953526765 >>END_MODULE >>Per sequence quality scores fail #Quality Count 2 145847.0 3 8493.0 4 19193.0 5 10440.0 6 8884.0 7 10851.0 8 13935.0 9 16413.0 10 17147.0 11 16387.0 12 15230.0 13 14406.0 14 13846.0 15 14939.0 16 16345.0 17 17098.0 18 17733.0 19 18440.0 20 19381.0 21 20448.0 22 21568.0 23 23263.0 24 24467.0 25 25727.0 26 27376.0 27 28803.0 28 31060.0 29 32736.0 30 34204.0 31 34633.0 32 33620.0 33 33198.0 34 30873.0 35 26867.0 36 19528.0 37 8770.0 38 1162.0 39 11.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.03642324342235 21.25174088610766 14.679070844972914 23.032765025497078 2 14.657627018830146 21.471224769223973 42.091338721787906 21.779809490157973 3 17.741994020117197 27.24755095602748 31.39201174759423 23.618443276261093 4 11.890487968074174 17.851190049465913 40.63552942863288 29.622792553827036 5 12.237055770197145 38.15220681541304 36.73487646635164 12.875860948038179 6 28.537117092054903 39.44452784040126 18.757342095876584 13.261012971667258 7 24.77803497103859 33.63204881600539 24.62236059944142 16.9675556135146 8 23.446386197674265 37.37850203862239 21.913289720361316 17.26182204334203 9 25.06458541665434 16.96072804302933 22.427204572754007 35.54748196756232 10-11 23.42103806483754 27.264485734142724 30.26126907393398 19.053207127085756 12-13 23.94943536835493 25.29421282306305 30.2481238430167 20.50822796556532 14-15 21.232382291842676 26.790968004850463 28.0727246071425 23.903925096164357 16-17 20.61690415034882 29.745978729014677 28.696492924321 20.9406241963155 18-19 20.53618998806801 28.667905108606767 30.901017156540007 19.894887746785216 20-21 21.110719306219984 27.899860105270953 30.930527557359216 20.058893031149847 22-23 20.93433493345491 27.73410061416041 30.736912975828535 20.59465147655614 24-25 20.792513525369205 28.000623144062438 30.65233344220134 20.55452988836702 26-27 20.680901480799697 28.16951899687036 30.549122433847074 20.60045708848287 28-29 20.785404406742344 28.224517593944675 30.588502893308984 20.401575106003996 30-31 20.994780719838293 28.12980822413741 30.443119232226124 20.432291823798174 32-33 20.791788508810157 28.22509186711098 30.329641971716388 20.653477652362476 34-35 21.093351434389625 28.16991175735058 30.320460147904683 20.416276660355116 36-37 21.75150267674993 28.103059816499247 29.820317741398117 20.32511976535271 38-39 21.416382572842757 28.1696199661715 29.95188485397665 20.462112607009093 40-41 21.2929254423584 28.058070459671526 30.129363138035586 20.51964095993449 42-43 21.417189586209037 28.131801129675576 29.728928671233923 20.722080612881463 44-45 21.71528048526172 28.361183898353747 29.30603282830572 20.61750278807881 46-47 20.9528324247452 28.354438089991934 29.8136522594647 20.879077225798166 48-49 21.578431213748562 28.414574347934284 29.132350970607163 20.87464346770999 50-51 21.775091271694468 28.56290036919878 28.927766861399196 20.73424149770756 52-53 20.71513158023087 28.71978258876227 29.53585510079461 21.02923073021224 54-55 20.56438175008431 28.68644626467453 29.554710590704637 21.194461394536525 56-57 20.60441171528708 28.83000656940607 29.490373546840125 21.07520816846672 58-59 20.72134148090388 28.562099655878313 29.539301177511156 21.177257685706643 60-61 20.740275594645492 28.705733197940493 29.44767801939615 21.106313188017868 62-63 20.66389464554104 28.631424920381637 29.43403097336344 21.270649460713884 64-65 20.54559937348467 28.43423466432081 29.48678578325144 21.533380178943077 66-67 20.628617778997 28.62343653069508 29.23958142144876 21.50836426885916 68-69 20.606692610320458 28.6339760647153 29.305407946642376 21.453923378321864 70-71 20.57536516629645 28.56864236282457 29.126323519209603 21.729668951669378 72-73 20.775064203021294 28.616250205560167 28.972446162736055 21.636239428682487 74-75 20.83675799680596 28.73173440564315 28.8954029745153 21.536104623035587 76-77 20.860990059843928 28.55800723228007 28.841937334986774 21.73906537288923 78-79 20.724194437127302 28.67017497302099 28.91306777663536 21.692562813216348 80-81 20.86479156327706 28.695921104165073 28.745875040620266 21.693412291937605 82-83 21.021212788962334 28.524805645265428 28.77188879844735 21.682092767324892 84-85 21.06482224501928 28.634029784824605 28.67338252532891 21.6277654448272 86-87 20.966813155455927 28.61422205198274 28.617499733286717 21.801465059274616 88-89 21.04834144536699 28.473303499875147 28.487618853385943 21.99073620137192 90-91 21.127369477567452 28.42966780494277 28.468710738838322 21.974251978651452 92-93 21.256499246087454 28.303436801331046 28.41366401497426 22.026399937607238 94-95 21.12460532830374 28.235234415306852 28.385653696039846 22.254506560349554 96-97 21.20165866462104 28.02841798814149 28.295418750723613 22.474504596513857 98-99 21.15765225847514 28.087670383137926 28.16921521179363 22.585462146593308 100-101 21.505792778923972 28.149886174593497 28.06027412494092 22.284046921541613 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 11571.0 1 12341.0 2 9268.0 3 5904.0 4 7607.5 5 9275.0 6 9623.5 7 9278.0 8 8574.5 9 7604.5 10 6632.5 11 5711.5 12 4851.0 13 4134.0 14 3570.5 15 3165.5 16 2865.0 17 2811.0 18 2964.5 19 3040.5 20 3318.5 21 3801.0 22 4414.5 23 5254.5 24 6218.5 25 7488.5 26 8965.0 27 10683.0 28 12778.5 29 15076.5 30 17362.5 31 19608.0 32 21529.0 33 22811.0 34 24139.5 35 25375.5 36 26260.0 37 26995.5 38 27496.0 39 27588.5 40 27344.0 41 27062.0 42 27001.5 43 26862.5 44 26456.5 45 26139.5 46 25482.5 47 24733.0 48 24166.5 49 23214.0 50 21724.5 51 20230.0 52 18880.0 53 17512.5 54 15873.0 55 13900.5 56 12016.0 57 10353.5 58 8764.0 59 7222.0 60 5886.0 61 4756.5 62 3772.0 63 2946.0 64 2242.5 65 1695.0 66 1311.0 67 968.0 68 706.0 69 530.5 70 386.5 71 297.5 72 217.5 73 151.5 74 109.0 75 70.0 76 51.5 77 37.0 78 26.5 79 22.5 80 12.0 81 5.0 82 3.0 83 2.5 84 2.0 85 2.0 86 2.0 87 0.5 88 0.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 14.986912043896753 2 22.224563219522697 3 17.968172106050233 4 23.33274553944593 5 23.20965233899982 6 16.855295068714632 7 15.706692376923975 8 12.658790228575487 9 3.242446657704718 10-11 6.9123416105399835 12-13 3.752796792019438 14-15 7.837887972592012 16-17 12.065939023636185 18-19 15.214949354304597 20-21 15.734345407535825 22-23 13.602771944368744 24-25 16.20811109762493 26-27 13.599050522029676 28-29 13.665177334362355 30-31 14.602804005853512 32-33 13.030817957179597 34-35 19.872051774717686 36-37 24.7325728654494 38-39 23.738724090312623 40-41 18.89847043816599 42-43 18.870473891645922 44-45 24.22657393263882 46-47 13.52548086501886 48-49 22.238189350548822 50-51 22.029446183652766 52-53 11.960823155720341 54-55 13.586683949333695 56-57 13.111028921749366 58-59 14.168027371347566 60-61 13.566760026656835 62-63 13.52960305591752 64-65 14.612021682724126 66-67 12.970416409983946 68-69 14.022777394821153 70-71 12.521727381195022 72-73 11.917998172495368 74-75 11.953208553088093 76-77 12.00278934917476 78-79 12.409569437160634 80-81 12.437279720423852 82-83 10.882755730417875 84-85 10.817201444598899 86-87 10.916134026166752 88-89 12.413405364802443 90-91 9.816253340692208 92-93 11.908780495624752 94-95 12.00055649577132 96-97 11.483794064503128 98-99 11.394937949576445 100-101 11.449213463075475 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 873322.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 98.60810284022557 #Duplication Level Percentage of deduplicated Percentage of total 1 99.32022926024607 97.93779381009125 2 0.5459821936659168 1.0767653660388137 3 0.07303903640741762 0.21606722440240844 4 0.024350245203641492 0.09604525932901559 5 0.01119365455145776 0.0551892519584053 6 0.005599435208689007 0.033128980974335136 7 0.0044566258622375865 0.03076215949407531 8 0.002054827603682387 0.016209812133027766 9 0.0026217039714702462 0.02326691293518293 >10 0.009180685078932854 0.1637023557304663 >50 8.225137216637538E-4 0.05677086148566879 >100 2.3490927437236014E-4 0.02999732459457692 >500 1.1745460223871673E-4 0.0786416136220034 >1k 1.1745460223871673E-4 0.1856590672107091 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 1603 0.18355200029313357 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 1.1450530274056992E-4 0.0 0.0 0.0 0.0 7 1.1450530274056992E-4 0.0 0.0 0.0 0.0 8 1.1450530274056992E-4 0.0 0.0 0.0 0.0 9 1.1450530274056992E-4 0.0 0.0 0.0 0.0 10-11 1.1450530274056992E-4 0.0 0.0 0.0 0.0 12-13 1.1450530274056992E-4 0.0 0.0 0.0 0.0 14-15 1.1450530274056992E-4 0.0 0.0 0.0 1.1450530274056992E-4 16-17 1.1450530274056992E-4 0.0 0.0 0.0 2.2901060548113984E-4 18-19 1.1450530274056992E-4 0.0 0.0 0.0 2.2901060548113984E-4 20-21 1.1450530274056992E-4 0.0 0.0 5.725265137028496E-5 2.2901060548113984E-4 22-23 2.2901060548113984E-4 0.0 0.0 4.0076855959199467E-4 2.2901060548113984E-4 24-25 2.2901060548113984E-4 0.0 0.0 9.160424219245594E-4 2.2901060548113984E-4 26-27 2.2901060548113984E-4 0.0 0.0 0.001259558330146269 2.2901060548113984E-4 28-29 2.2901060548113984E-4 0.0 0.0 0.0032634011281062426 2.2901060548113984E-4 30-31 2.2901060548113984E-4 0.0 0.0 0.01316810981516554 2.2901060548113984E-4 32-33 2.2901060548113984E-4 0.0 0.0 0.03979059270234805 2.2901060548113984E-4 34-35 2.2901060548113984E-4 5.725265137028496E-5 0.0 0.07328339375396475 2.2901060548113984E-4 36-37 2.2901060548113984E-4 1.1450530274056992E-4 0.0 0.12544055915229435 2.2901060548113984E-4 38-39 2.2901060548113984E-4 1.1450530274056992E-4 0.0 0.20055603775010822 2.2901060548113984E-4 40-41 2.2901060548113984E-4 2.2901060548113984E-4 0.0 0.28185480269591284 2.2901060548113984E-4 42-43 2.2901060548113984E-4 2.2901060548113984E-4 0.0 0.3593176399999084 2.2901060548113984E-4 44-45 2.2901060548113984E-4 2.2901060548113984E-4 0.0 0.45790670565953906 2.2901060548113984E-4 46-47 2.2901060548113984E-4 2.2901060548113984E-4 0.0 0.5796830951241352 2.2901060548113984E-4 48-49 2.2901060548113984E-4 2.2901060548113984E-4 0.0 0.6987113573229576 2.2901060548113984E-4 50-51 2.2901060548113984E-4 2.2901060548113984E-4 0.0 0.8338848672082004 2.2901060548113984E-4 52-53 2.2901060548113984E-4 2.2901060548113984E-4 0.0 1.0112535811533432 2.2901060548113984E-4 54-55 3.435159082217098E-4 2.2901060548113984E-4 0.0 1.198641509088286 2.2901060548113984E-4 56-57 3.435159082217098E-4 2.2901060548113984E-4 0.0 1.4107053297638212 2.862632568514248E-4 58-59 3.435159082217098E-4 2.2901060548113984E-4 0.0 1.6394869246394799 3.435159082217098E-4 60-61 3.435159082217098E-4 2.2901060548113984E-4 0.0 1.8776579543398655 3.435159082217098E-4 62-63 3.435159082217098E-4 2.2901060548113984E-4 0.0 2.113996899196402 3.435159082217098E-4 64-65 3.435159082217098E-4 2.2901060548113984E-4 0.0 2.3583512152447783 3.435159082217098E-4 66-67 3.435159082217098E-4 2.2901060548113984E-4 0.0 2.6165034202733928 3.435159082217098E-4 68-69 3.435159082217098E-4 2.2901060548113984E-4 0.0 2.894007021465164 3.435159082217098E-4 70-71 3.435159082217098E-4 2.2901060548113984E-4 0.0 3.1793542358946643 3.435159082217098E-4 72-73 3.435159082217098E-4 2.2901060548113984E-4 0.0 3.466819798424865 3.435159082217098E-4 74-75 3.435159082217098E-4 2.2901060548113984E-4 0.0 3.7583502992023563 3.435159082217098E-4 76-77 4.580212109622797E-4 2.2901060548113984E-4 0.0 4.074957461280032 3.435159082217098E-4 78-79 4.580212109622797E-4 2.2901060548113984E-4 0.0 4.425343687666176 3.435159082217098E-4 80-81 4.580212109622797E-4 2.2901060548113984E-4 0.0 4.789699560986669 3.435159082217098E-4 82-83 4.580212109622797E-4 2.2901060548113984E-4 0.0 5.152681370674276 3.435159082217098E-4 84-85 4.580212109622797E-4 2.2901060548113984E-4 0.0 5.51314406370159 3.435159082217098E-4 86-87 4.580212109622797E-4 2.2901060548113984E-4 0.0 5.9053247255880414 3.435159082217098E-4 88-89 4.580212109622797E-4 2.2901060548113984E-4 0.0 6.301913841630006 3.435159082217098E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 730 0.0 62.988277 1 GTATCAA 1540 0.0 48.888504 1 TCAACGC 1885 0.0 38.567398 4 ATCAACG 1910 0.0 38.08334 3 TATCAAC 2000 0.0 37.38859 2 CAACGCA 2025 0.0 35.13912 5 AACGCAG 2160 0.0 32.237965 6 GTACATG 1315 0.0 30.35586 1 GTGGTAT 365 0.0 29.071514 1 TACATGG 1345 0.0 28.549513 2 ACGCAGA 2590 0.0 26.372997 7 CGCAGAG 2655 0.0 25.938328 8 ACATGGG 1440 0.0 25.25666 3 GCAGAGT 2720 0.0 23.411823 9 CATGGGG 970 0.0 21.859829 4 TGGTATC 395 0.0 20.465872 2 AGAGTAC 2455 0.0 20.22014 10-11 GTACTAC 200 7.5251937E-6 20.211624 1 GAGTACT 2015 0.0 20.122562 12-13 CAGAGTA 2590 0.0 19.762476 10-11 >>END_MODULE