FastQCFastQC Report
Sat 18 Jun 2016
SRR1930908_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1930908_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences873322
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23310.26691186068826844No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19540.22374336155507363No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14120.16168148746968472No Hit
CTTATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGT13930.1595058867176139No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA9700.057.2986531
GTATCAA20450.041.580531
CAACGCA25850.031.9426695
TCAACGC26050.031.7174024
ATCAACG26200.031.5358123
AACGCAG26900.030.5194236
TATCAAC27400.030.3314632
ACGCAGA30750.026.6982927
CGCAGAG31400.026.1456188
GTACATG17850.022.8870891
ACATGGG17850.022.3458653
GCAGAGT36800.021.7932199
TACATGG18600.021.4472792
AGAGTAC33900.021.13779810-11
GACCGTG1805.2089345E-621.0911377
CAGAGTA35100.019.7391419
GTCCTAC2752.8321665E-819.0016041
AGTACTT30800.018.48898312-13
GAGTACT27600.018.13952312-13
CATGGGA9350.017.775124
Produced by FastQC (version 0.11.5)