FastQCFastQC Report
Sat 18 Jun 2016
SRR1930907_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1930907_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1634011
Sequences flagged as poor quality0
Sequence length101
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN29810.18243451237476369No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA12850.053.3003431
GTATCAA22450.044.6406971
ATCAACG29600.032.9960143
TATCAAC29650.032.943082
TCAACGC29950.032.9344334
CAACGCA31500.030.235435
AACGCAG33650.028.5176096
GTACATG38400.027.9346121
TACATGG40400.026.919042
ACATGGG45550.023.099863
ACGCAGA40900.023.0300547
CTACTAG2653.8198777E-1022.8049761
CGCAGAG43600.022.071968
GTGGTAT6600.020.6022221
CATGGGG37750.020.130334
GCAGAGT45850.019.574959
GTCCTAA3353.3469405E-1019.543071
GGATCGT1901.2511431E-519.0589396
AGTACAT28100.018.8135222
GTATAGC3757.4578566E-1118.8014351
Produced by FastQC (version 0.11.5)