##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1930907_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1634011 Sequences flagged as poor quality 0 Sequence length 101 %GC 51 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 22.159464654766705 28.0 10.0 31.0 2.0 33.0 2 21.11940923286318 28.0 2.0 31.0 2.0 33.0 3 21.555682305688272 28.0 10.0 31.0 2.0 33.0 4 23.44732440601685 32.0 2.0 35.0 2.0 37.0 5 23.394442265076552 32.0 2.0 35.0 2.0 37.0 6 24.070872839901323 32.0 10.0 35.0 2.0 37.0 7 24.08458388591019 32.0 10.0 35.0 2.0 37.0 8 24.767582347976848 32.0 11.0 35.0 2.0 37.0 9 25.841857245759055 32.0 16.0 37.0 2.0 39.0 10-11 25.997764090939413 32.5 15.5 37.0 2.0 39.0 12-13 25.856545029378626 32.0 11.0 37.0 2.0 39.0 14-15 26.028189528711863 32.5 10.5 38.0 2.0 40.0 16-17 25.14124660115507 32.0 10.0 38.0 2.0 40.0 18-19 24.50423191765539 31.5 8.0 37.5 2.0 40.0 20-21 23.946529735723935 31.0 2.0 37.0 2.0 39.5 22-23 23.822893787128727 30.0 2.0 37.0 2.0 39.0 24-25 23.47185269866604 30.0 2.0 37.0 2.0 39.0 26-27 23.07692298277062 30.0 2.0 37.0 2.0 39.0 28-29 22.92654700610951 30.0 2.0 37.0 2.0 39.0 30-31 22.755710028879854 30.0 2.0 37.0 2.0 39.0 32-33 22.366038233524744 30.0 2.0 36.5 2.0 39.0 34-35 19.64422577326591 23.5 2.0 35.0 2.0 38.0 36-37 18.04160222911596 16.5 2.0 34.0 2.0 38.0 38-39 17.118831513374147 15.0 2.0 33.0 2.0 38.0 40-41 18.177919242893715 15.5 2.0 33.0 2.0 38.0 42-43 18.01737931996786 16.0 2.0 33.0 2.0 38.0 44-45 16.206565928870734 10.5 2.0 32.0 2.0 37.5 46-47 16.829843862740212 14.5 2.0 31.5 2.0 37.0 48-49 16.267429350230813 8.5 2.0 32.0 2.0 37.0 50-51 14.492894172683048 7.0 2.0 30.0 2.0 36.5 52-53 17.00273682368111 16.0 2.0 32.0 2.0 37.0 54-55 18.361722167109036 20.5 2.0 34.0 2.0 38.0 56-57 18.476930693857017 21.5 2.0 34.0 2.0 38.0 58-59 18.08726073447486 19.5 2.0 34.0 2.0 38.0 60-61 17.691495956881564 18.0 2.0 34.0 2.0 38.0 62-63 17.16676693118957 13.5 2.0 33.0 2.0 37.0 64-65 16.648181376991953 9.0 2.0 32.5 2.0 36.0 66-67 16.18478272178094 7.5 2.0 32.0 2.0 36.0 68-69 15.687411223057861 4.0 2.0 31.0 2.0 35.0 70-71 15.10905526339786 2.0 2.0 31.0 2.0 35.0 72-73 14.610110641850024 2.0 2.0 30.5 2.0 34.5 74-75 14.077700211320487 2.0 2.0 30.0 2.0 34.0 76-77 13.521960378479704 2.0 2.0 29.5 2.0 34.0 78-79 13.025222902416202 2.0 2.0 29.0 2.0 34.0 80-81 12.52425044874239 2.0 2.0 28.5 2.0 34.0 82-83 12.067546362906981 2.0 2.0 27.5 2.0 33.5 84-85 11.570847748270973 2.0 2.0 26.5 2.0 33.0 86-87 11.040934852947746 2.0 2.0 25.5 2.0 33.0 88-89 10.517045172890512 2.0 2.0 24.5 2.0 32.0 90-91 9.983661064705196 2.0 2.0 23.5 2.0 32.0 92-93 9.415332271324978 2.0 2.0 19.0 2.0 32.0 94-95 8.81703642141944 2.0 2.0 12.0 2.0 31.0 96-97 8.17611907141384 2.0 2.0 2.0 2.0 31.0 98-99 7.490496698002644 2.0 2.0 2.0 2.0 31.0 100-101 6.669726213593421 2.0 2.0 2.0 2.0 28.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 7.093203002567314 1101 2 7.993516924197191 1101 3 7.8016460251630235 1101 4 9.67884861115613 1101 5 9.588193548247233 1101 6 8.99455456885898 1101 7 8.811157735960961 1101 8 7.947096895073287 1101 9 8.252423126047251 1101 10-11 7.892379429717106 1101 12-13 7.550739086062794 1101 14-15 7.9543343658822785 1101 16-17 8.55017605873915 1101 18-19 9.196078283104775 1101 20-21 9.45948716105191 1101 22-23 9.381789428120353 1101 24-25 9.516818255258055 1101 26-27 9.44903442604453 1101 28-29 9.367393815523489 1101 30-31 9.100086172823072 1101 32-33 8.97380521893621 1101 34-35 11.357675391113403 1101 36-37 5.556851934219338 1101 38-39 8.54391929848337 1101 40-41 10.099547090696287 1101 42-43 10.43965909675672 1101 44-45 5.148760231529842 1101 46-47 6.477949866849649 1101 48-49 9.410937215589165 1101 50-51 -2.553653213177336 1101 52-53 6.403135206632172 1101 54-55 8.871196984483571 1101 56-57 9.3857646650583 1101 58-59 9.54577125285093 1101 60-61 9.103998111290728 1101 62-63 9.267410396808895 1101 64-65 9.03818926187207 1101 66-67 9.225296168668713 1101 68-69 9.08408742124112 1101 70-71 9.050700398642087 1101 72-73 9.006113294504608 1101 74-75 9.029092670181884 1101 76-77 8.93973383427304 1101 78-79 8.614775837125302 1101 80-81 8.302130655667147 1101 82-83 8.013057150555618 1101 84-85 8.068866761901495 1101 86-87 7.717192463311056 1101 88-89 7.526795963450031 1101 90-91 7.33443551151429 1101 92-93 7.306339717101816 1101 94-95 7.140236985857802 1101 96-97 6.892685491591285 1101 98-99 6.263469510985751 1101 100-101 5.659944948889936 1102 1 6.739495760608147 1102 2 7.602442324064469 1102 3 7.45539779425761 1102 4 9.01486884451555 1102 5 9.138340981744669 1102 6 8.674340394769331 1102 7 8.180355338368003 1102 8 7.441309083338595 1102 9 7.248974062727807 1102 10-11 7.005948143605917 1102 12-13 6.7700887393309905 1102 14-15 7.424652054823728 1102 16-17 7.953604791340819 1102 18-19 8.100075595727503 1102 20-21 8.473175138940107 1102 22-23 8.449892142629114 1102 24-25 8.24680323629823 1102 26-27 8.411835118434329 1102 28-29 8.38051178164994 1102 30-31 8.250236577182434 1102 32-33 8.175122456163468 1102 34-35 10.09127784397155 1102 36-37 1.0480531718159902 1102 38-39 3.3903350430493795 1102 40-41 8.106615594477159 1102 42-43 8.92861196456911 1102 44-45 1.1909751057406837 1102 46-47 1.9200454412675896 1102 48-49 6.953160501276553 1102 50-51 -3.9600213094132197 1102 52-53 4.641044514449977 1102 54-55 7.537368209216034 1102 56-57 8.036991738415683 1102 58-59 8.07832241431477 1102 60-61 7.946418583464489 1102 62-63 8.168642302291104 1102 64-65 7.998264814828218 1102 66-67 8.13036621300256 1102 68-69 7.70237712108384 1102 70-71 7.532329733863408 1102 72-73 7.49816122775113 1102 74-75 7.3487869434338915 1102 76-77 7.141754414113704 1102 78-79 7.3693133813034475 1102 80-81 7.20407699975309 1102 82-83 7.072606825156754 1102 84-85 7.130310092531223 1102 86-87 6.876727569950679 1102 88-89 6.7866005236873015 1102 90-91 6.368672726606842 1102 92-93 6.237699562931978 1102 94-95 5.887931883348747 1102 96-97 5.774971530473067 1102 98-99 5.317540450873403 1102 100-101 4.81012792534997 1103 1 6.714932614885981 1103 2 7.691703366545362 1103 3 7.266806518207794 1103 4 9.109338302712398 1103 5 9.026202385001277 1103 6 8.12540872690031 1103 7 7.901310176758226 1103 8 7.379992685236417 1103 9 7.6476559865990374 1103 10-11 7.061470707905631 1103 12-13 6.886326294253696 1103 14-15 7.278966164895397 1103 16-17 7.904828203387812 1103 18-19 8.246646919766128 1103 20-21 8.330361494108189 1103 22-23 8.221879104912716 1103 24-25 8.449087449988042 1103 26-27 8.469257841527625 1103 28-29 8.224519610606762 1103 30-31 8.032240808010123 1103 32-33 8.131131510629874 1103 34-35 10.137716542015479 1103 36-37 -2.6235955374445865 1103 38-39 -1.4821445644574531 1103 40-41 6.320510686180622 1103 42-43 8.47049093483847 1103 44-45 0.9454323638589983 1103 46-47 0.842972958083962 1103 48-49 6.7732531847367845 1103 50-51 -4.29748414696993 1103 52-53 4.104383244060063 1103 54-55 6.877559043477209 1103 56-57 7.385454762343379 1103 58-59 7.486586798406801 1103 60-61 7.409079053353199 1103 62-63 7.592820086456996 1103 64-65 7.343708594404298 1103 66-67 7.248714367757877 1103 68-69 7.034719570548765 1103 70-71 6.969118334836251 1103 72-73 6.946402875958356 1103 74-75 6.941809963345326 1103 76-77 6.679999494022843 1103 78-79 6.3689961723843265 1103 80-81 6.236141921501348 1103 82-83 6.324152294519358 1103 84-85 6.046672330330976 1103 86-87 5.613953248708993 1103 88-89 5.601086260117466 1103 90-91 5.424273174722465 1103 92-93 5.081098419535666 1103 94-95 4.757421474426048 1103 96-97 4.680571359644459 1103 98-99 4.574629769340155 1103 100-101 4.082177125914721 1104 1 6.35687864226837 1104 2 7.464460310156731 1104 3 7.176271664271098 1104 4 8.940066183114002 1104 5 8.593728473598297 1104 6 7.8964191703813285 1104 7 7.91934550485027 1104 8 6.838097792911569 1104 9 7.0461083301328 1104 10-11 6.5985116233236845 1104 12-13 6.460701498241026 1104 14-15 6.936865760573244 1104 16-17 7.471611895392581 1104 18-19 8.067002693771961 1104 20-21 8.002209299288317 1104 22-23 8.014926043946726 1104 24-25 7.962681467119289 1104 26-27 8.05839820960621 1104 28-29 7.996654623243089 1104 30-31 7.516061555171724 1104 32-33 7.339804599448723 1104 34-35 9.54807588140147 1104 36-37 -2.762520374271478 1104 38-39 -1.748674826291733 1104 40-41 5.959291600941899 1104 42-43 7.964964428046489 1104 44-45 0.5413374041136123 1104 46-47 0.21841251517260218 1104 48-49 6.011176464725686 1104 50-51 -4.556059656963383 1104 52-53 3.4709668190506626 1104 54-55 6.3332479694198405 1104 56-57 6.792972520550286 1104 58-59 6.846420110182542 1104 60-61 6.7000040215361985 1104 62-63 6.920784127051398 1104 64-65 7.137043210023478 1104 66-67 6.9623101273681485 1104 68-69 6.770814587929996 1104 70-71 6.805832971056896 1104 72-73 6.831158831718156 1104 74-75 6.675292937649791 1104 76-77 6.3958782362478654 1104 78-79 6.1020044887005636 1104 80-81 6.136658432495386 1104 82-83 5.950279628184653 1104 84-85 5.550660596745294 1104 86-87 5.376698980138022 1104 88-89 5.13668209076727 1104 90-91 4.854359219677086 1104 92-93 4.679113869963141 1104 94-95 4.530251970757794 1104 96-97 4.200976961848561 1104 98-99 3.629636146143203 1104 100-101 3.3776794524593123 1105 1 5.866161476386829 1105 2 6.618488558852231 1105 3 6.440559547740126 1105 4 8.454782750846771 1105 5 8.328362538961201 1105 6 7.293890674323642 1105 7 7.338325661198983 1105 8 6.148671389913446 1105 9 6.293882564545196 1105 10-11 5.898692879528326 1105 12-13 5.755163121529829 1105 14-15 5.992810950910371 1105 16-17 6.501083732664494 1105 18-19 7.374651092966438 1105 20-21 7.5605530134891445 1105 22-23 7.34326047630157 1105 24-25 7.471752182401136 1105 26-27 7.498215912570011 1105 28-29 7.214826422373136 1105 30-31 7.119366392996334 1105 32-33 6.790200576568875 1105 34-35 8.54743672518649 1105 36-37 -3.353076672389351 1105 38-39 -2.3179533653331266 1105 40-41 4.517009498699306 1105 42-43 6.571336589886574 1105 44-45 -3.0880076559219454 1105 46-47 -4.035416328641402 1105 48-49 3.8081845021850818 1105 50-51 -8.631757425828948 1105 52-53 0.2114142251073794 1105 54-55 4.70377231137385 1105 56-57 5.56182968749901 1105 58-59 5.855703932432224 1105 60-61 5.824164357026515 1105 62-63 5.816720404444865 1105 64-65 6.0310562546731745 1105 66-67 5.660219307568372 1105 68-69 5.54381564786542 1105 70-71 5.609996890169791 1105 72-73 5.552208563045276 1105 74-75 5.641118583871146 1105 76-77 5.561996599876208 1105 78-79 5.269380743380188 1105 80-81 5.178202763026595 1105 82-83 5.177786303011089 1105 84-85 5.074052956646403 1105 86-87 4.957335099496371 1105 88-89 4.779662702973852 1105 90-91 4.706373722808472 1105 92-93 4.32712077634166 1105 94-95 3.8799033580610978 1105 96-97 3.8589677814507137 1105 98-99 3.523515463700252 1105 100-101 3.122899038274226 1106 1 5.69799475999087 1106 2 6.861867717809236 1106 3 6.517099583824436 1106 4 7.505607277658218 1106 5 7.667531028403925 1106 6 6.745471822300807 1106 7 6.671948387626081 1106 8 6.22195591603009 1106 9 6.111765564237729 1106 10-11 5.579837804754195 1106 12-13 5.633009095356531 1106 14-15 6.1080612634906295 1106 16-17 6.34335659615288 1106 18-19 6.609001869214168 1106 20-21 6.9426963773559365 1106 22-23 6.653427102438048 1106 24-25 6.856056416577072 1106 26-27 6.7095323405414895 1106 28-29 6.626097623204043 1106 30-31 6.130729022131508 1106 32-33 5.9507137439005255 1106 34-35 8.159958149636875 1106 36-37 -0.4418415104141751 1106 38-39 0.4677292414384766 1106 40-41 5.1996675688542915 1106 42-43 6.417635902648325 1106 44-45 -3.446192648238526 1106 46-47 -4.327537390625311 1106 48-49 3.291257603594847 1106 50-51 -8.636979813166128 1106 52-53 -0.44871241737969925 1106 54-55 3.859489388823949 1106 56-57 4.893875795156486 1106 58-59 5.3021987316357375 1106 60-61 5.505975514071835 1106 62-63 5.360105342605756 1106 64-65 4.978869069505858 1106 66-67 5.074022156845761 1106 68-69 4.594970855528777 1106 70-71 4.488777926199228 1106 72-73 4.470969190043441 1106 74-75 4.208930561043941 1106 76-77 4.270072290564121 1106 78-79 4.15173141183603 1106 80-81 3.728067877057109 1106 82-83 3.6396278106170747 1106 84-85 3.3407057488947363 1106 86-87 3.3038483726718706 1106 88-89 3.2672494275580988 1106 90-91 2.780312143118284 1106 92-93 2.773780185453777 1106 94-95 2.3768923278985223 1106 96-97 2.2069674139193207 1106 98-99 2.0037746122936477 1106 100-101 1.9451479343001798 1107 1 5.881520608332206 1107 2 7.113916924867997 1107 3 6.706800653965015 1107 4 7.787667509058025 1107 5 8.021689495750081 1107 6 6.658105908036138 1107 7 6.706414645665657 1107 8 6.121960847553503 1107 9 6.331203402385132 1107 10-11 5.816614083808425 1107 12-13 5.420769054249899 1107 14-15 5.771298506355432 1107 16-17 6.450282045836229 1107 18-19 6.866396529487883 1107 20-21 7.121779400347975 1107 22-23 6.871139066472924 1107 24-25 7.094941478674496 1107 26-27 7.17686895201221 1107 28-29 6.912535933823204 1107 30-31 6.914891832399896 1107 32-33 6.855791940362874 1107 34-35 8.766872622703595 1107 36-37 0.1631754397090326 1107 38-39 0.988154656919324 1107 40-41 6.382188181826745 1107 42-43 7.203277851777269 1107 44-45 -2.983651863744509 1107 46-47 -3.890002452372757 1107 48-49 4.198922940871281 1107 50-51 -8.462036088211505 1107 52-53 0.30359688776864857 1107 54-55 4.781781942700842 1107 56-57 6.001742052241578 1107 58-59 6.543082333365906 1107 60-61 6.207127581573257 1107 62-63 6.127945128545825 1107 64-65 5.8041531491165586 1107 66-67 5.694587245796125 1107 68-69 5.81466386024236 1107 70-71 5.994339411955876 1107 72-73 5.760913654726579 1107 74-75 5.58898795761319 1107 76-77 5.618524508079302 1107 78-79 5.63624308761541 1107 80-81 5.153460609264252 1107 82-83 5.008103763077187 1107 84-85 4.840297705102808 1107 86-87 4.597529341286702 1107 88-89 4.4110334013796635 1107 90-91 4.172335960785546 1107 92-93 3.800406116676525 1107 94-95 3.5992489915797634 1107 96-97 3.1706317903440926 1107 98-99 3.1335700813372034 1107 100-101 2.786737380203756 1108 1 5.953896874421975 1108 2 6.230854837569662 1108 3 6.47860929079069 1108 4 7.85876523461328 1108 5 7.927461781050951 1108 6 7.226063526726897 1108 7 7.345627725804601 1108 8 6.467728293944507 1108 9 4.453810380784425 1108 10-11 5.817715250017123 1108 12-13 5.269227123053536 1108 14-15 6.078171486542608 1108 16-17 6.183579715775444 1108 18-19 6.682048517604272 1108 20-21 7.256135189051001 1108 22-23 7.580469678704997 1108 24-25 7.22641701625696 1108 26-27 7.436793823223372 1108 28-29 7.4849968808355705 1108 30-31 7.399643924678536 1108 32-33 7.12248908587738 1108 34-35 9.164163304502853 1108 36-37 -3.008816855740978 1108 38-39 -2.1917000815203167 1108 40-41 4.909234545810175 1108 42-43 7.167841006620815 1108 44-45 -2.619976994497195 1108 46-47 -3.849710201069943 1108 48-49 4.599847361184018 1108 50-51 -8.59946599864454 1108 52-53 0.5931362015546746 1108 54-55 5.242601383806754 1108 56-57 6.457643401323363 1108 58-59 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2313 74-75 -11.849724987272817 2313 76-77 -11.336801870995842 2313 78-79 -10.83957929289742 2313 80-81 -10.327886240489462 2313 82-83 -9.871116782753115 2313 84-85 -9.377920055212176 2313 86-87 -8.848220909899798 2313 88-89 -8.34260451430336 2313 90-91 -7.816120896401657 2313 92-93 -7.2861172171197 2313 94-95 -6.711057415105474 2313 96-97 -6.04801482496889 2313 98-99 -5.375793661219099 2313 100-101 -4.560171187910672 2314 1 -19.951348472447737 2314 2 -18.90112485786181 2314 3 -19.312140850213257 2314 4 -21.16943555500883 2314 5 -21.157140587815377 2314 6 -21.795933089178348 2314 7 -21.808348585532116 2314 8 -22.51647926386711 2314 9 -23.595258993158044 2314 10-11 -23.749402629283498 2314 12-13 -23.5708743997891 2314 14-15 -23.732601215093037 2314 16-17 -22.851389263355973 2314 18-19 -22.23137505825585 2314 20-21 -21.70607575285537 2314 22-23 -21.544459819320945 2314 24-25 -21.205337072857535 2314 26-27 -20.816167259686953 2314 28-29 -20.67715765949156 2314 30-31 -20.492629058965008 2314 32-33 -20.092118923146874 2314 34-35 -17.455690051391112 2314 36-37 -15.862172749706009 2314 38-39 -14.971733922467862 2314 40-41 -16.02477560647409 2314 42-43 -15.849925490841112 2314 44-45 -14.065267462630302 2314 46-47 -14.659918886913147 2314 48-49 -14.111940568878023 2314 50-51 -12.363707397403706 2314 52-53 -14.803366900532186 2314 54-55 -16.12880301551643 2314 56-57 -16.239762126031405 2314 58-59 -15.870845243235767 2314 60-61 -15.46975264126797 2314 62-63 -14.929157935521026 2314 64-65 -14.42147359183052 2314 66-67 -13.97337932802003 2314 68-69 -13.465280429451235 2314 70-71 -12.92070237077393 2314 72-73 -12.416523671178716 2314 74-75 -11.888727919824136 2314 76-77 -11.355570199441589 2314 78-79 -10.855121814891556 2314 80-81 -10.361024070401486 2314 82-83 -9.907773674248716 2314 84-85 -9.414576946707777 2314 86-87 -8.885757566791293 2314 88-89 -8.378381640403067 2314 90-91 -7.851898022501363 2314 92-93 -7.310457393072779 2314 94-95 -6.711057415105474 2314 96-97 -6.059451775115518 2314 98-99 -5.40570568467951 2314 100-101 -4.596828079406273 2315 1 -19.951348472447737 2315 2 -18.90112485786181 2315 3 -19.312140850213257 2315 4 -17.34143555500883 2315 5 -21.157140587815377 2315 6 -21.795933089178348 2315 7 -21.808348585532116 2315 8 -22.51647926386711 2315 9 -19.987830421729473 2315 10-11 -18.633974057854928 2315 12-13 -15.998588685503385 2315 14-15 -15.97202978652161 2315 16-17 -19.712817834784545 2315 18-19 -22.23137505825585 2315 20-21 -21.70607575285537 2315 22-23 -21.544459819320945 2315 24-25 -21.205337072857535 2315 26-27 -20.816167259686953 2315 28-29 -20.67715765949156 2315 30-31 -20.492629058965008 2315 32-33 -20.092118923146874 2315 34-35 -17.455690051391112 2315 36-37 -15.862172749706009 2315 38-39 -14.971733922467862 2315 40-41 -16.02477560647409 2315 42-43 -15.849925490841112 2315 44-45 -14.065267462630302 2315 46-47 -14.659918886913147 2315 48-49 -14.111940568878023 2315 50-51 -12.363707397403706 2315 52-53 -14.803366900532186 2315 54-55 -16.12880301551643 2315 56-57 -16.239762126031405 2315 58-59 -15.870845243235767 2315 60-61 -15.46975264126797 2315 62-63 -14.929157935521026 2315 64-65 -14.42147359183052 2315 66-67 -13.97337932802003 2315 68-69 -13.465280429451235 2315 70-71 -12.92070237077393 2315 72-73 -12.416523671178716 2315 74-75 -11.888727919824136 2315 76-77 -11.355570199441589 2315 78-79 -10.855121814891556 2315 80-81 -10.361024070401486 2315 82-83 -9.907773674248716 2315 84-85 -9.414576946707777 2315 86-87 -8.885757566791293 2315 88-89 -8.378381640403067 2315 90-91 -7.851898022501363 2315 92-93 -7.310457393072779 2315 94-95 -6.711057415105474 2315 96-97 -6.059451775115518 2315 98-99 -5.40570568467951 2315 100-101 -4.596828079406273 2316 1 -19.951348472447737 2316 2 -18.90112485786181 2316 3 -19.312140850213257 2316 4 -21.16943555500883 2316 5 -21.157140587815377 2316 6 -21.795933089178348 2316 7 -21.808348585532116 2316 8 -22.51647926386711 2316 9 -23.595258993158044 2316 10-11 -23.749402629283498 2316 12-13 -23.5708743997891 2316 14-15 -23.732601215093037 2316 16-17 -22.851389263355973 2316 18-19 -22.23137505825585 2316 20-21 -21.70607575285537 2316 22-23 -21.544459819320945 2316 24-25 -21.205337072857535 2316 26-27 -20.816167259686953 2316 28-29 -20.67715765949156 2316 30-31 -20.492629058965008 2316 32-33 -20.092118923146874 2316 34-35 -17.455690051391112 2316 36-37 -15.862172749706009 2316 38-39 -14.971733922467862 2316 40-41 -16.02477560647409 2316 42-43 -15.849925490841112 2316 44-45 -14.065267462630302 2316 46-47 -14.659918886913147 2316 48-49 -14.111940568878023 2316 50-51 -12.363707397403706 2316 52-53 -14.803366900532186 2316 54-55 -16.12880301551643 2316 56-57 -16.239762126031405 2316 58-59 -15.870845243235767 2316 60-61 -15.46975264126797 2316 62-63 -14.929157935521026 2316 64-65 -14.42147359183052 2316 66-67 -13.97337932802003 2316 68-69 -13.465280429451235 2316 70-71 -12.92070237077393 2316 72-73 -12.416523671178716 2316 74-75 -11.888727919824136 2316 76-77 -11.355570199441589 2316 78-79 -10.855121814891556 2316 80-81 -10.361024070401486 2316 82-83 -9.907773674248716 2316 84-85 -9.414576946707777 2316 86-87 -8.885757566791293 2316 88-89 -8.378381640403067 2316 90-91 -7.851898022501363 2316 92-93 -7.310457393072779 2316 94-95 -6.711057415105474 2316 96-97 -6.059451775115518 2316 98-99 -5.40570568467951 2316 100-101 -4.596828079406273 >>END_MODULE >>Per sequence quality scores fail #Quality Count 2 302788.0 3 20016.0 4 38055.0 5 27082.0 6 25094.0 7 30821.0 8 38499.0 9 44658.0 10 45337.0 11 41966.0 12 35670.0 13 30928.0 14 29233.0 15 30172.0 16 32538.0 17 34504.0 18 36078.0 19 37402.0 20 39298.0 21 40728.0 22 42586.0 23 44534.0 24 46246.0 25 48194.0 26 49703.0 27 51838.0 28 53444.0 29 54516.0 30 54145.0 31 51814.0 32 49148.0 33 45003.0 34 37630.0 35 27205.0 36 13641.0 37 3317.0 38 180.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.5028748423739 17.099407855469213 12.724666390895544 25.673050911261335 2 17.185990987725628 19.17099859149111 37.43609290390571 26.206917516877553 3 20.649756677857464 22.652559062399664 27.557027933589108 29.140656326153763 4 14.505999324108787 15.258481013457814 33.904077140032655 36.33144252240074 5 15.884835435832706 35.693635580265386 32.47835283673825 15.943176147163657 6 35.32796208323076 32.710299490171984 16.474991105952036 15.486747320645222 7 30.706912133770693 30.03899484749522 19.767039092064568 19.487053926669525 8 28.21768525560133 32.931636288675875 18.945224902988215 19.905453552734574 9 28.126234459223248 14.953473902348833 18.16776908213658 38.75252255629134 10-11 27.54773197229521 24.086826411009376 25.96114533819674 22.404296278498673 12-13 27.89045723002655 21.493628876376434 25.942260829386914 24.6736530642101 14-15 25.023727139384672 22.603740739308243 23.785420048879065 28.587112072428024 16-17 25.370681436733022 25.267944980303657 24.240372867613182 25.12100071535014 18-19 25.317104939163237 24.689472105267118 25.630867592805323 24.362555362764322 20-21 26.094945331078907 23.967947161661083 25.640361563393544 24.29674594386647 22-23 25.868994251358053 23.714747815691858 25.328832867464797 25.0874250654853 24-25 25.64228511648866 23.978523602737365 25.250125696765792 25.12906558400818 26-27 25.493162822022253 24.233751190198884 25.275601855337964 24.9974841324409 28-29 25.418767513903777 24.491485277586357 25.229524086391013 24.860223122118853 30-31 25.75887844687469 24.216650488870375 25.076460968741372 24.948010095513567 32-33 25.567455379733683 24.320983041033497 25.008407716450243 25.103153862782573 34-35 25.81158875134335 24.308891442061356 25.103481140805943 24.776038665789347 36-37 26.184563264993727 24.400563152202153 24.73905871405524 24.67581486874888 38-39 26.101688312669165 24.337651403760454 24.69548321803771 24.86517706553267 40-41 26.170917762624107 24.120012293767196 24.767977578082995 24.941092365525705 42-43 26.08223393650077 24.108737533235985 24.70683581460485 25.10219271565839 44-45 26.13684273685474 24.313886277725555 24.59909198183964 24.95017900358007 46-47 25.657554581075615 24.116904364344894 24.755846674906028 25.46969437967347 48-49 26.07109664430734 24.36152293010498 24.492020572294173 25.075359853293506 50-51 26.071866242414625 24.554325411266316 24.451683789155446 24.92212455716361 52-53 25.446600414729946 24.409982491939257 24.739896993552783 25.40352009977801 54-55 25.210481419595464 24.1965800194777 24.97776085150994 25.61517770941689 56-57 25.35490678368784 24.405518908796154 24.66298180395226 25.576592503563745 58-59 25.419438589693645 24.40238529735967 24.744030923454268 25.43414518949242 60-61 25.48944771821631 24.347160186751882 24.721977123351113 25.441414971680693 62-63 25.35911196936944 24.364685851828998 24.722332487827202 25.553869690974352 64-65 25.221363619081526 24.17583159486629 24.782420678110523 25.820384107941656 66-67 25.333427205763954 24.39335522605713 24.446855540785364 25.826362027393557 68-69 25.227548730965005 24.34225586547527 24.56750309600308 25.862692307556646 70-71 25.161024041744234 24.184656869092098 24.56004118327634 26.094277905887324 72-73 25.370938032542263 24.309411685646495 24.342977463716046 25.976672818095192 74-75 25.327706119250397 24.392772736616855 24.37935544576448 25.900165698368266 76-77 25.36643675368576 24.141691178601942 24.45422698029061 26.037645087421684 78-79 25.309676327550857 24.304459339116733 24.301164910100233 26.084699423232177 80-81 25.521621768711256 24.25030357379937 24.22641977730873 26.00165488018064 82-83 25.600167410904472 24.306890908210974 24.287157416009965 25.80578426487459 84-85 25.571631974679725 24.249189755968153 24.13388088586908 26.04529738348304 86-87 25.29893752783729 24.383077929403612 24.203793851616762 26.114190691142337 88-89 25.375585430892734 24.22359258341578 24.153210924924498 26.24761106076699 90-91 25.482110459801167 24.260101498357205 24.043240213452403 26.21454782838922 92-93 25.553933313324272 24.05139318522725 24.204769162434843 26.18990433901363 94-95 25.525735912995724 23.950666854318616 24.159740102049547 26.363857130636113 96-97 25.377266326925017 24.062214874807513 24.140204874786917 26.42031392348055 98-99 25.254182923979172 23.90772448798461 24.050210851032187 26.78788173700403 100-101 26.09193410879973 23.844819807843063 23.636949984647345 26.426296098709862 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 17770.0 1 20026.0 2 15014.5 3 7526.5 4 9538.5 5 13126.5 6 14661.0 7 14463.5 8 13609.0 9 12584.0 10 11605.0 11 10599.0 12 9418.5 13 8226.5 14 6969.5 15 5774.0 16 4889.0 17 4128.0 18 3442.0 19 3075.5 20 2959.0 21 3037.5 22 3365.0 23 3793.0 24 4254.5 25 4825.5 26 5398.0 27 5997.0 28 6685.5 29 7255.0 30 7906.5 31 8654.0 32 9306.5 33 10185.0 34 11040.5 35 11870.5 36 13244.0 37 14871.0 38 16635.0 39 19009.5 40 21988.5 41 25455.5 42 30015.5 43 35860.0 44 42050.0 45 48848.0 46 55961.5 47 62057.0 48 67073.5 49 70935.5 50 73073.0 51 73873.0 52 73858.0 53 72350.0 54 69487.0 55 65298.0 56 59975.5 57 54039.0 58 47583.5 59 41511.0 60 35574.0 61 29655.0 62 24483.5 63 19769.5 64 15539.0 65 12168.5 66 9422.0 67 7142.5 68 5406.5 69 4130.5 70 3170.5 71 2393.0 72 1760.5 73 1285.5 74 920.0 75 642.0 76 487.5 77 349.5 78 220.0 79 134.0 80 80.5 81 48.0 82 29.0 83 22.0 84 13.0 85 6.5 86 3.5 87 3.0 88 2.5 89 1.5 90 1.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 14.486866979475657 2 21.486636258874633 3 17.276811478013308 4 22.492810635913713 5 22.374390380480914 6 16.224982573556726 7 15.016177981665974 8 12.1237249932834 9 3.017666343739424 10-11 6.520733336556487 12-13 3.575587924438697 14-15 7.4358434551542185 16-17 11.540007992602252 18-19 14.627043514394947 20-21 15.050571874975137 22-23 12.970292121656463 24-25 15.526517263347674 26-27 13.037243935322346 28-29 13.057072443208767 30-31 13.907342117035931 32-33 12.379996217895718 34-35 19.221688226089054 36-37 24.037996072241864 38-39 23.020499861996033 40-41 18.16071005641945 42-43 18.10593686333813 44-45 23.50265695885768 46-47 12.824118075092517 48-49 21.54180724609565 50-51 21.296245863705938 52-53 11.284685354015364 54-55 12.903523905285827 56-57 12.41919424043045 58-59 13.44421793978131 60-61 12.850739682902992 62-63 12.816345789593827 64-65 13.884882047917671 66-67 12.205548187864096 68-69 13.247003845139355 70-71 11.84940003463869 72-73 11.207299094069747 74-75 11.284440557621705 76-77 11.325321555362846 78-79 11.761426330667296 80-81 11.726389846824777 82-83 10.217923869545553 84-85 10.14561713476837 86-87 10.275451022055544 88-89 11.699033849833326 90-91 9.158567475983943 92-93 11.199526808571056 94-95 11.29239644041564 96-97 10.857576846177903 98-99 10.705191091124846 100-101 10.808280972404715 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1634011.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 98.15801274081988 #Duplication Level Percentage of deduplicated Percentage of total 1 99.04632469950556 97.2219040178545 2 0.7365973188028379 1.4460585800780543 3 0.12043030342930466 0.35463597775183475 4 0.0389221515599555 0.15282084194888937 5 0.018111571343233414 0.08888979253326869 6 0.010040195436033687 0.059131537891830975 7 0.009212543241526998 0.06329994558140128 8 0.0032890020174821096 0.0258273521549273 9 0.003152849335043755 0.02785296826791994 >10 0.012847111868621733 0.21453782755345127 >50 5.719152307417431E-4 0.04018504400540096 >100 3.7528853914265016E-4 0.07961667008504435 >500 6.25248452677084E-5 0.042286097307413985 >1k 6.25248452677084E-5 0.18295334698606835 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 2981 0.18243451237476369 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 1.223981968297643E-4 2 0.0 0.0 0.0 0.0 1.223981968297643E-4 3 0.0 0.0 0.0 0.0 1.223981968297643E-4 4 0.0 0.0 0.0 0.0 1.223981968297643E-4 5 0.0 0.0 0.0 0.0 1.223981968297643E-4 6 0.0 0.0 0.0 0.0 1.8359729524464646E-4 7 0.0 0.0 0.0 0.0 1.8359729524464646E-4 8 0.0 0.0 0.0 0.0 1.8359729524464646E-4 9 0.0 0.0 0.0 0.0 1.8359729524464646E-4 10-11 0.0 0.0 0.0 3.059954920744108E-5 3.059954920744108E-4 12-13 0.0 0.0 0.0 6.119909841488215E-5 4.283936889041751E-4 14-15 3.059954920744108E-5 0.0 0.0 6.119909841488215E-5 4.895927873190572E-4 16-17 6.119909841488215E-5 3.059954920744108E-5 0.0 6.119909841488215E-5 5.201923365264983E-4 18-19 6.119909841488215E-5 6.119909841488215E-5 0.0 9.179864762232323E-5 6.731900825637036E-4 20-21 6.119909841488215E-5 6.119909841488215E-5 0.0 1.529977460372054E-4 7.343891809785859E-4 22-23 6.119909841488215E-5 6.119909841488215E-5 0.0 5.813914349413804E-4 8.567873778083502E-4 24-25 6.119909841488215E-5 6.119909841488215E-5 0.0 0.001101583771467879 8.567873778083502E-4 26-27 6.119909841488215E-5 9.179864762232323E-5 0.0 0.00198897069848367 8.567873778083502E-4 28-29 6.119909841488215E-5 1.223981968297643E-4 0.0 0.003671945904892929 8.567873778083502E-4 30-31 1.223981968297643E-4 1.223981968297643E-4 0.0 0.01052624492735973 8.873869270157912E-4 32-33 1.529977460372054E-4 1.223981968297643E-4 0.0 0.028396381664505317 9.179864762232323E-4 34-35 1.8359729524464646E-4 1.223981968297643E-4 0.0 0.052172231398687034 0.0010403846730529967 36-37 1.8359729524464646E-4 1.223981968297643E-4 0.0 0.0889528895460312 0.0010403846730529967 38-39 1.8359729524464646E-4 1.223981968297643E-4 0.0 0.14158411418282985 0.0011627828698827609 40-41 1.8359729524464646E-4 1.223981968297643E-4 0.0 0.19687749960067588 0.0011627828698827609 42-43 1.8359729524464646E-4 1.223981968297643E-4 0.0 0.25094690305022427 0.0011627828698827609 44-45 2.1419684445208754E-4 1.223981968297643E-4 0.0 0.31627694060811096 0.001193382419090202 46-47 2.447963936595286E-4 1.529977460372054E-4 0.0 0.38944046276310257 0.001223981968297643 48-49 2.447963936595286E-4 1.8359729524464646E-4 0.0 0.4674999127912848 0.0013157806159199662 50-51 2.447963936595286E-4 1.8359729524464646E-4 0.0 0.55437203299121 0.0013463801651274073 52-53 2.447963936595286E-4 2.447963936595286E-4 0.0 0.6668253763285559 0.0013463801651274073 54-55 2.447963936595286E-4 2.447963936595286E-4 0.0 0.7862860164344059 0.001529977460372054 56-57 2.447963936595286E-4 2.447963936595286E-4 0.0 0.9200366460201308 0.0016523756572018181 58-59 3.059954920744108E-4 2.447963936595286E-4 0.0 1.0690564506603688 0.0016523756572018181 60-61 3.059954920744108E-4 2.447963936595286E-4 0.0 1.2170664701767615 0.0016523756572018181 62-63 3.059954920744108E-4 2.447963936595286E-4 0.0 1.3640973041185158 0.0017135747556167003 64-65 3.059954920744108E-4 2.447963936595286E-4 0.0 1.5159934663842534 0.0017441743048241414 66-67 3.059954920744108E-4 2.447963936595286E-4 0.0 1.6836177969426154 0.0017747738540315823 68-69 3.059954920744108E-4 2.447963936595286E-4 0.0 1.8631147525934648 0.0017747738540315823 70-71 3.059954920744108E-4 2.447963936595286E-4 0.0 2.051638575260509 0.0018359729524464645 72-73 3.059954920744108E-4 2.447963936595286E-4 0.0 2.248485475311978 0.001958371149276229 74-75 3.059954920744108E-4 2.447963936595286E-4 0.0 2.4422418208934946 0.0020807693461059934 76-77 3.059954920744108E-4 2.447963936595286E-4 0.0 2.651573337021599 0.0020807693461059934 78-79 3.059954920744108E-4 2.447963936595286E-4 0.0 2.8860270830490125 0.0021113688953134343 80-81 3.059954920744108E-4 2.447963936595286E-4 0.0 3.128375512771946 0.002141968444520875 82-83 3.059954920744108E-4 2.447963936595286E-4 0.0 3.3756198703680695 0.002141968444520875 84-85 3.059954920744108E-4 2.447963936595286E-4 0.0 3.6261077801801824 0.002141968444520875 86-87 3.059954920744108E-4 2.447963936595286E-4 0.0 3.884826968729097 0.002141968444520875 88-89 3.059954920744108E-4 2.447963936595286E-4 0.0 4.15324621437677 0.002141968444520875 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1285 0.0 53.300343 1 GTATCAA 2245 0.0 44.640697 1 ATCAACG 2960 0.0 32.996014 3 TATCAAC 2965 0.0 32.94308 2 TCAACGC 2995 0.0 32.934433 4 CAACGCA 3150 0.0 30.23543 5 AACGCAG 3365 0.0 28.517609 6 GTACATG 3840 0.0 27.934612 1 TACATGG 4040 0.0 26.91904 2 ACATGGG 4555 0.0 23.09986 3 ACGCAGA 4090 0.0 23.030054 7 CTACTAG 265 3.8198777E-10 22.804976 1 CGCAGAG 4360 0.0 22.07196 8 GTGGTAT 660 0.0 20.602222 1 CATGGGG 3775 0.0 20.13033 4 GCAGAGT 4585 0.0 19.57495 9 GTCCTAA 335 3.3469405E-10 19.54307 1 GGATCGT 190 1.2511431E-5 19.058939 6 AGTACAT 2810 0.0 18.813522 2 GTATAGC 375 7.4578566E-11 18.801435 1 >>END_MODULE