FastQCFastQC Report
Sat 18 Jun 2016
SRR1930907_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1930907_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1634011
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGT28810.17631460253327547No Hit
ATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTT21580.13206765437931567RNA PCR Primer, Index 46 (95% over 21bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19410.11878745002328625No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16760.1025696889433425No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA16250.054.93191
GTATCAA29100.042.7492451
ATCAACG38400.031.4009573
CAACGCA38450.031.3495675
TCAACGC39000.031.2820784
TATCAAC39450.030.927142
AACGCAG40750.029.5792376
GTACATG54850.025.4501781
TACATGG55600.024.5907272
GTGGTAT7150.024.5706061
TATCACG1401.7877028E-523.7368832
ACGCAGA50650.023.6103277
GTATAAT2503.8562575E-1022.7908951
ACATGGG59800.022.1484553
CGCAGAG54650.021.6217128
CATGGGG46050.020.5140084
GTATACG2953.0340743E-919.314321
GTATAAG3951.8189894E-1219.2328221
CTAGTAC3951.8189894E-1219.229293
TGGTATC8250.018.9895082
Produced by FastQC (version 0.11.5)