##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1930907_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1634011 Sequences flagged as poor quality 0 Sequence length 101 %GC 50 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.206265441297518 31.0 31.0 33.0 30.0 34.0 2 31.317429319631263 31.0 31.0 34.0 30.0 34.0 3 31.402426911446742 31.0 31.0 34.0 28.0 34.0 4 35.2741670649708 37.0 35.0 37.0 33.0 37.0 5 35.03958847278262 35.0 35.0 37.0 32.0 37.0 6 35.080397255587634 36.0 35.0 37.0 32.0 37.0 7 34.94574455129127 36.0 35.0 37.0 32.0 37.0 8 34.92163822642565 36.0 35.0 37.0 32.0 37.0 9 36.425260295065335 38.0 35.0 39.0 32.0 39.0 10-11 36.34423452473698 38.0 35.0 39.0 32.0 39.0 12-13 36.21930146125087 38.0 35.0 39.0 32.0 39.0 14-15 37.22992409475823 39.0 36.0 40.0 32.0 41.0 16-17 37.1702133584168 39.0 36.0 40.0 31.0 41.0 18-19 37.1247788417581 39.0 36.0 40.0 31.0 41.0 20-21 37.07008092356783 39.0 36.0 40.0 31.0 41.0 22-23 36.92564064746198 39.0 36.0 40.0 31.0 41.0 24-25 36.77672396330257 38.5 35.5 40.0 30.5 41.0 26-27 36.48078103513379 38.0 35.0 40.0 30.0 41.0 28-29 36.26486602599371 38.0 35.0 40.0 30.0 41.0 30-31 36.0030538350109 38.0 34.0 40.0 29.0 41.0 32-33 35.753127120931254 38.0 34.0 40.0 28.5 41.0 34-35 35.98596551675601 38.0 34.5 40.0 30.0 41.0 36-37 36.03857685168582 38.0 35.0 40.0 29.5 41.0 38-39 35.99064602380278 38.0 35.0 40.0 29.0 41.0 40-41 35.873049814230136 38.0 34.5 40.0 29.0 41.0 42-43 35.70446159787174 38.0 34.0 40.0 28.0 41.0 44-45 35.50505718749751 38.0 34.0 40.0 27.5 41.0 46-47 35.25678101310211 38.0 34.0 40.0 27.0 41.0 48-49 35.02465007885503 38.0 33.0 40.0 26.0 41.0 50-51 34.693644963222404 37.5 33.0 40.0 26.0 41.0 52-53 34.42583097665805 37.0 33.0 40.0 26.0 41.0 54-55 34.18778851549959 37.0 33.0 40.0 25.0 41.0 56-57 33.84668401865104 36.5 32.0 39.5 24.5 41.0 58-59 33.529072325706494 36.0 32.0 39.0 23.5 41.0 60-61 33.13126717017205 35.5 31.0 39.0 23.0 40.0 62-63 32.73407308763527 35.0 31.0 39.0 22.0 40.0 64-65 32.34223668016923 35.0 31.0 38.0 22.0 40.0 66-67 31.925495605598737 35.0 30.5 37.5 21.0 40.0 68-69 31.479222600092655 34.5 30.0 37.0 20.0 39.0 70-71 31.03565184077708 34.0 30.0 36.0 19.5 39.0 72-73 30.584476787487965 34.0 29.5 36.0 18.0 38.5 74-75 30.059494397528535 34.0 29.0 35.0 16.5 37.0 76-77 28.741877196665136 32.5 27.0 34.5 13.0 36.0 78-79 29.498245421848445 33.5 29.0 35.0 15.0 36.0 80-81 29.527331517352085 34.0 29.0 35.0 14.0 36.0 82-83 29.30032508961078 34.0 29.0 35.0 10.5 35.5 84-85 29.033635942475296 33.0 29.0 35.0 7.5 35.0 86-87 28.738518896139624 33.0 29.0 35.0 6.5 35.0 88-89 28.427856054824602 33.0 28.0 35.0 2.0 35.0 90-91 28.106813846418415 33.0 27.0 35.0 2.0 35.0 92-93 27.74142462933236 33.0 27.0 35.0 2.0 35.0 94-95 27.314112022501682 33.0 26.0 35.0 2.0 35.0 96-97 26.823041888946893 33.0 25.0 35.0 2.0 35.0 98-99 26.295756270918616 32.5 25.0 34.0 2.0 35.0 100-101 25.029483889643338 31.5 21.0 34.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.497455343815286 1101 2 0.519966598308077 1101 3 0.46512511879533847 1101 4 0.41253950874284584 1101 5 0.41278774472853996 1101 6 0.46978115590622593 1101 7 0.5373509631139299 1101 8 0.41788250587439535 1101 9 0.5668432550933815 1101 10-11 0.6102637460240672 1101 12-13 0.5498258447349187 1101 14-15 0.668613966794247 1101 16-17 0.6623052613032883 1101 18-19 0.6894437861222187 1101 20-21 0.748694796490696 1101 22-23 0.8132747740324078 1101 24-25 0.7241526044745967 1101 26-27 0.733295451943242 1101 28-29 0.6073278378949212 1101 30-31 0.7084001396233646 1101 32-33 0.7025343842848031 1101 34-35 0.97559168322217 1101 36-37 0.8102704664019171 1101 38-39 0.8535658488820062 1101 40-41 0.8778902299268978 1101 42-43 0.8445444559943027 1101 44-45 0.803777433260997 1101 46-47 0.7947432828909271 1101 48-49 0.8453563997208136 1101 50-51 0.8941003352968764 1101 52-53 0.9501669844388303 1101 54-55 1.0133911245310046 1101 56-57 1.1090655026733174 1101 58-59 0.9707625429805873 1101 60-61 0.997275270617699 1101 62-63 1.0868072007183116 1101 64-65 1.2803694550965332 1101 66-67 1.2987370521340473 1101 68-69 1.2608236435971882 1101 70-71 1.101764584856955 1101 72-73 1.1270699836297986 1101 74-75 1.0783824327796054 1101 76-77 1.264736038877512 1101 78-79 1.270151018972438 1101 80-81 1.1253156554863537 1101 82-83 1.1882362971909224 1101 84-85 1.1767478794170714 1101 86-87 1.1300278125685672 1101 88-89 1.1871158371739945 1101 90-91 1.1021367317352997 1101 92-93 1.3133109263814084 1101 94-95 1.3238495693505996 1101 96-97 1.1993172850111122 1101 98-99 1.212036770723099 1101 100-101 1.383306337120164 1102 1 0.46857462857490617 1102 2 0.5489378711470252 1102 3 0.5561913432450325 1102 4 0.27567977423051815 1102 5 0.46807799078033696 1102 6 0.380459149550731 1102 7 0.4441521609081889 1102 8 0.5974657404772827 1102 9 0.6527562868687156 1102 10-11 0.5901655071367031 1102 12-13 0.7048702967039588 1102 14-15 0.8356376141393298 1102 16-17 0.7469741104200054 1102 18-19 0.8565411681127912 1102 20-21 0.816445481757583 1102 22-23 0.8313660704091745 1102 24-25 0.8575979953791801 1102 26-27 0.8476247780730048 1102 28-29 0.9160410179985945 1102 30-31 0.9072354543665 1102 32-33 0.9239992554531966 1102 34-35 0.9708944999572111 1102 36-37 0.9126245344425286 1102 38-39 0.960649493320652 1102 40-41 0.909851156291765 1102 42-43 0.8867485921499778 1102 44-45 1.2019325280028639 1102 46-47 1.1408880705315667 1102 48-49 0.9355608952433982 1102 50-51 0.997741717184347 1102 52-53 1.122394114349973 1102 54-55 1.1778192049747815 1102 56-57 1.0176800000500137 1102 58-59 1.0771455293196297 1102 60-61 1.0516953762658403 1102 62-63 0.8350169879544822 1102 64-65 0.8191660484127326 1102 66-67 0.8013626855898259 1102 68-69 0.6586431741151024 1102 70-71 0.6829197633556454 1102 72-73 0.6734123872790185 1102 74-75 0.855412802147228 1102 76-77 0.961649599681035 1102 78-79 1.0425400429619707 1102 80-81 0.8637695616686827 1102 82-83 1.0852468942196296 1102 84-85 1.0569491248882947 1102 86-87 0.9807835210976599 1102 88-89 0.9325267503310464 1102 90-91 0.9816532472220807 1102 92-93 1.392790431371381 1102 94-95 1.3454352078461014 1102 96-97 1.3646267808164687 1102 98-99 1.4111514538681433 1102 100-101 1.4678255693349023 1103 1 0.3799290917473819 1103 2 0.48588739762030286 1103 3 0.47396013181299423 1103 4 0.23904343353885338 1103 5 0.4341013909986202 1103 6 0.41229420228821567 1103 7 0.34382577098295286 1103 8 0.46792552663892195 1103 9 0.3850257696857895 1103 10-11 0.49856262038080246 1103 12-13 0.5574097561180622 1103 14-15 0.6642072546496465 1103 16-17 0.6754172799921605 1103 18-19 0.47010230501705763 1103 20-21 0.6448376915879308 1103 22-23 0.5557273371541953 1103 24-25 0.6373821076309056 1103 26-27 0.6809676821180872 1103 28-29 0.561950071708516 1103 30-31 0.7775011327396868 1103 32-33 0.8037425173855866 1103 34-35 0.6385059735327658 1103 36-37 0.7723693643669662 1103 38-39 0.6753951175568602 1103 40-41 0.7403916183955275 1103 42-43 0.7976728890809142 1103 44-45 0.7284211060747694 1103 46-47 0.8286234842510822 1103 48-49 0.916749976516968 1103 50-51 0.9843695989715044 1103 52-53 0.8155901635601666 1103 54-55 0.88861837201339 1103 56-57 1.0044823897249202 1103 58-59 0.8284331052520528 1103 60-61 0.6655903991977254 1103 62-63 0.6055557926631394 1103 64-65 0.7177513482121256 1103 66-67 0.7748947389923337 1103 68-69 0.7042813709517439 1103 70-71 0.6436468921627565 1103 72-73 0.6142758763776932 1103 74-75 0.8610843944637878 1103 76-77 0.8223852561642353 1103 78-79 1.0594530379712168 1103 80-81 0.9090305463814481 1103 82-83 0.9759055815489504 1103 84-85 0.8664776338035018 1103 86-87 0.870409946990435 1103 88-89 0.8960893314385316 1103 90-91 0.9639363124264797 1103 92-93 0.9228305458348025 1103 94-95 1.0388755680991508 1103 96-97 0.9324947036189926 1103 98-99 1.0106663391330777 1103 100-101 1.1728480954663851 1104 1 0.4342337856507825 1104 2 0.4878369635754787 1104 3 0.4082435406649232 1104 4 0.19936081939140138 1104 5 0.3062624067834676 1104 6 0.38241772394555795 1104 7 0.4816089489564064 1104 8 0.4436739046255198 1104 9 0.47620902687502564 1104 10-11 0.5498338928663173 1104 12-13 0.38809792810342003 1104 14-15 0.534508006529343 1104 16-17 0.7426133028508062 1104 18-19 0.7900274856484657 1104 20-21 0.6868723173268876 1104 22-23 0.5947657367006229 1104 24-25 0.6215505128671879 1104 26-27 0.7231874462534407 1104 28-29 0.7707558570069537 1104 30-31 0.8063051487517328 1104 32-33 0.8585034392106081 1104 34-35 0.6748676572232242 1104 36-37 0.8330831977068982 1104 38-39 0.7807289798889556 1104 40-41 0.7042928144733409 1104 42-43 0.48530973232549 1104 44-45 0.6760473805138716 1104 46-47 0.8854845959392961 1104 48-49 0.7080324879921989 1104 50-51 0.6309571548564961 1104 52-53 0.687919898227527 1104 54-55 0.5497929905277701 1104 56-57 0.618620294876429 1104 58-59 0.8362608571793331 1104 60-61 0.5113940740859917 1104 62-63 0.4592559133633145 1104 64-65 0.5964493410773457 1104 66-67 0.583708097372682 1104 68-69 0.5090471516125064 1104 70-71 0.6219101365112572 1104 72-73 0.8205565750045558 1104 74-75 0.8429559107623348 1104 76-77 1.0084473339900484 1104 78-79 1.089366711539217 1104 80-81 0.9010747892642605 1104 82-83 0.9083066104157922 1104 84-85 1.021831534644381 1104 86-87 1.0611959859804152 1104 88-89 1.0927543941309175 1104 90-91 1.0040166809205822 1104 92-93 0.9396005660810651 1104 94-95 0.935764204254113 1104 96-97 1.062675646662047 1104 98-99 1.11543875461782 1104 100-101 1.4321113820364957 1105 1 0.15633670017677304 1105 2 0.25186266289241743 1105 3 0.20577841675656217 1105 4 0.07640666275152341 1105 5 0.15468175913591153 1105 6 0.21142326209170648 1105 7 0.18911420087387398 1105 8 0.17718248852148122 1105 9 0.32657038646454595 1105 10-11 0.16800994096064414 1105 12-13 0.27732001850161936 1105 14-15 0.3680782168761354 1105 16-17 0.31396000054918005 1105 18-19 0.3658336195718661 1105 20-21 0.3423830922391602 1105 22-23 0.33497533250883293 1105 24-25 0.4368439186722597 1105 26-27 0.5188226400622042 1105 28-29 0.3675283239819578 1105 30-31 0.3817323343497847 1105 32-33 0.4963670138609473 1105 34-35 0.3237801858707954 1105 36-37 0.5089124209632843 1105 38-39 0.3794528413476854 1105 40-41 0.34332814806973744 1105 42-43 0.3348463317179835 1105 44-45 0.316522585995763 1105 46-47 0.3366264474468892 1105 48-49 0.36840651542403435 1105 50-51 0.4945451449778133 1105 52-53 0.25026257950184316 1105 54-55 0.2650402506045637 1105 56-57 0.4484731555492587 1105 58-59 0.32439274239537497 1105 60-61 0.43587383946411506 1105 62-63 0.20256313587795205 1105 64-65 0.1499131692162976 1105 66-67 0.18386656068370044 1105 68-69 0.2831572241610978 1105 70-71 0.15398026484315608 1105 72-73 0.2041537283961148 1105 74-75 0.4369215909885753 1105 76-77 0.4776260388398619 1105 78-79 0.5393791290549643 1105 80-81 0.49391762304016495 1105 82-83 0.5971439225144053 1105 84-85 0.6234939336820737 1105 86-87 0.46309497657142984 1105 88-89 0.46491310513349404 1105 90-91 0.4089123441443796 1105 92-93 0.4816989088532502 1105 94-95 0.48640378865929534 1105 96-97 0.31400517722724786 1105 98-99 0.5755487204482392 1105 100-101 0.8465920828455111 1106 1 0.024311761288608125 1106 2 0.08706217042632503 1106 3 0.1279465013303387 1106 4 -0.028836211538781242 1106 5 -0.026149530642790353 1106 6 0.05392177427138023 1106 7 0.01717379420622933 1106 8 0.11648493861172682 1106 9 0.14203878282312132 1106 10-11 -0.013882222341479178 1106 12-13 -6.316043670153704E-4 1106 14-15 0.030081964267779426 1106 16-17 -0.07112134174559515 1106 18-19 0.033837511768290085 1106 20-21 -0.011385192204159011 1106 22-23 8.318283827080108E-4 1106 24-25 -0.05086131205920452 1106 26-27 0.2171053398854852 1106 28-29 0.016209870042374064 1106 30-31 -0.04702696473017909 1106 32-33 -0.09096178939903865 1106 34-35 0.05717609477886043 1106 36-37 0.13798412172779706 1106 38-39 0.20095433696020137 1106 40-41 -0.03825986027699457 1106 42-43 0.10379392245553731 1106 44-45 -0.08792477113858155 1106 46-47 -0.0040683362371822795 1106 48-49 0.030574548049912664 1106 50-51 -0.24649327406576305 1106 52-53 -0.30674082535833946 1106 54-55 -0.05277180773682488 1106 56-57 -0.31838921400874654 1106 58-59 0.0323669723408031 1106 60-61 -0.08557220176498959 1106 62-63 -0.1921219121202853 1106 64-65 -0.21855555928417658 1106 66-67 -0.07676352189073654 1106 68-69 0.013044701762627398 1106 70-71 -0.04483814770044248 1106 72-73 0.021778124948202304 1106 74-75 0.20410262844658433 1106 76-77 0.2763831203938416 1106 78-79 0.4803778404223671 1106 80-81 0.20126279638219202 1106 82-83 0.3453836192165909 1106 84-85 0.2651834547191889 1106 86-87 0.1752781227895106 1106 88-89 0.1545576808473932 1106 90-91 0.11110331176817922 1106 92-93 0.04032698429069015 1106 94-95 0.2270241894438385 1106 96-97 0.29452958478579916 1106 98-99 0.3292617777802178 1106 100-101 0.3606939896905885 1107 1 0.06569074249950191 1107 2 0.08364287480694799 1107 3 0.055236120155395696 1107 4 0.016663614267088178 1107 5 -0.02311203038428289 1107 6 0.1930572304929683 1107 7 0.28816959604807835 1107 8 -0.05748807163789138 1107 9 0.03437917247710942 1107 10-11 0.11980342326652504 1107 12-13 0.12655111175700284 1107 14-15 0.03436873076332603 1107 16-17 0.07955801537465135 1107 18-19 0.08391904098596825 1107 20-21 0.11105562553786541 1107 22-23 0.28616404602514933 1107 24-25 0.2477761557466991 1107 26-27 0.12516329005801197 1107 28-29 0.3734969912894499 1107 30-31 0.11775400404373215 1107 32-33 0.4071510279484727 1107 34-35 0.19659742027689475 1107 36-37 0.4423272030390777 1107 38-39 0.4237308164569882 1107 40-41 0.34665556409650833 1107 42-43 0.41023767365859953 1107 44-45 0.6101676837896761 1107 46-47 0.6126735494182824 1107 48-49 0.6849507978331673 1107 50-51 0.6274210351471154 1107 52-53 0.36260705225715384 1107 54-55 0.6091628766218165 1107 56-57 0.5462403092720223 1107 58-59 0.4472434694956391 1107 60-61 0.3910181747647741 1107 62-63 0.37396750117160593 1107 64-65 0.5127818857730873 1107 66-67 0.4070613994832861 1107 68-69 0.6028517201158472 1107 70-71 0.3140580819112877 1107 72-73 0.3995735430857117 1107 74-75 0.5704853878407405 1107 76-77 0.5858118749932864 1107 78-79 0.41467197238674913 1107 80-81 0.4553235877485271 1107 82-83 0.39310231239470284 1107 84-85 0.3522167297757015 1107 86-87 0.45730271995563143 1107 88-89 0.5235178770902102 1107 90-91 0.4692668124498418 1107 92-93 0.613754663499865 1107 94-95 0.5114910042683185 1107 96-97 0.665235222153381 1107 98-99 0.741074049001277 1107 100-101 1.2660260800679133 1108 1 0.37915532340748825 1108 2 0.39864208141425905 1108 3 0.3883765255240732 1108 4 0.24292695465454273 1108 5 0.1589752051056692 1108 6 0.20754505230797093 1108 7 0.30325316936306024 1108 8 0.40997575829642585 1108 9 0.3839313376536069 1108 10-11 0.3428481030579462 1108 12-13 0.4526377062484954 1108 14-15 0.4709152259314848 1108 16-17 0.4558017958829552 1108 18-19 0.49813428135254156 1108 20-21 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-0.3151510163406641 2316 76-77 -0.5620577143779393 2316 78-79 -0.7840733720469153 2316 80-81 -0.8459201135440786 2316 82-83 -1.1397607044122822 2316 84-85 -0.9583224552263339 2316 86-87 -0.8197832867421262 2316 88-89 -0.8075883480670605 2316 90-91 -0.5482340091206872 2316 92-93 -0.8438497542022425 2316 94-95 -0.5389369948553409 2316 96-97 -0.5949739653524837 2316 98-99 -0.5208545682563965 2316 100-101 -0.7346959239382223 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4.0 3 11.0 4 42.0 5 71.0 6 142.0 7 427.0 8 954.0 9 1633.0 10 2573.0 11 3861.0 12 5119.0 13 6376.0 14 7449.0 15 8929.0 16 10336.0 17 11736.0 18 11304.0 19 11143.0 20 11440.0 21 12345.0 22 13859.0 23 15810.0 24 18989.0 25 23394.0 26 29260.0 27 36903.0 28 45275.0 29 55826.0 30 66584.0 31 79371.0 32 94454.0 33 113151.0 34 137780.0 35 170407.0 36 219788.0 37 257528.0 38 141507.0 39 8229.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.87992213945118 17.90278449399825 13.248596734998683 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25.52788812315217 24.53309677841826 20-21 25.73489407354051 24.138454392289894 25.386120411674096 24.740531122495504 22-23 25.485064350891367 24.032380462787867 25.315909939351656 25.16664524696911 24-25 25.41664575685954 24.28154922698993 25.268390124369493 25.033414891781035 26-27 25.290158879371198 24.469124537057013 25.26310829017938 24.977608293392404 28-29 25.21223114812438 24.571873402127345 25.236863822923812 24.97903162682446 30-31 25.528068858496063 24.478801200684373 25.028008114320233 24.965121826499335 32-33 25.57192448081016 24.351590394766077 24.96818475916827 25.10830036525549 34-35 25.413694400944554 24.395047204818017 25.2856481378052 24.90561025643223 36-37 25.392113033082325 24.417143334499183 25.17473230560964 25.01601132680885 38-39 25.288647490820072 24.22588739290086 25.208782129742964 25.27668298653611 40-41 25.415222462298193 24.070953415095165 25.359683005874945 25.154141116731697 42-43 25.445773221872305 24.09924997299364 25.145903189014824 25.309073616119232 44-45 25.184538234400357 24.1722824046263 25.231726652367332 25.411452708606006 46-47 25.392249178871012 24.176981336127504 24.95528278265489 25.475486702346593 48-49 25.353635476440473 24.210874862785793 24.93438047116306 25.501109189610677 50-51 25.183718428496583 24.16640378476364 25.10263124091601 25.547246545823764 52-53 25.37634257170026 24.143971814030895 25.173116052200868 25.306569562067978 54-55 25.082304218181083 24.072465049973204 25.126191639905237 25.719039091940477 56-57 25.17732818797492 24.318795961648515 24.857803878604635 25.64607197177193 58-59 25.14285084688287 24.45233112382529 24.95787162490964 25.44694640438221 60-61 25.33555243876564 24.633111269706117 24.739167853832964 25.292168437695278 62-63 25.065580700099243 24.676660499650808 24.660824338066824 25.596934462183125 64-65 24.900445392000098 24.58830340568656 24.763733941075923 25.74751726123742 66-67 24.953130743781397 24.64177184168607 24.625842421271905 25.77925499326063 68-69 24.94692030556014 24.56535304314902 24.695084687040406 25.79264196425043 70-71 24.883216680189673 24.368147948714885 24.769243633697183 25.97939173739826 72-73 24.98907549772706 24.45128682521868 24.578338710097242 25.981298966957016 74-75 24.888151290427555 24.510348478468032 24.577701537005385 26.023798694099032 76-77 24.920601799999385 24.296156053134656 24.52135543083688 26.261886716029075 78-79 24.82048953171821 24.407211510213674 24.586936191464776 26.185362766603344 80-81 24.904757970495623 24.55154391537306 24.519811872129353 26.023886242001964 82-83 24.987683681444008 24.42082703237616 24.566848081194067 26.024641204985766 84-85 24.919821506654944 24.410828449388788 24.398833422429046 26.270516621527218 86-87 24.76366438169633 24.49793789637891 24.56394112401936 26.174456597905397 88-89 24.765193784359553 24.334351076831375 24.547814054652154 26.352641084156925 90-91 24.811800678864024 24.363274264247757 24.44810166557929 26.376823391308925 92-93 24.720460472414533 24.219760686313727 24.643457986972624 26.41632085429912 94-95 24.677528281396256 24.175410906066507 24.669358049254566 26.47770276328267 96-97 24.530189821243553 24.121685839324215 24.708217998532444 26.63990634089979 98-99 24.371928952742667 24.092585668027937 24.717520261491508 26.817965117737884 100-101 24.440594341164164 24.217003435105394 24.56360452897808 26.77879769475236 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 123.0 1 87.5 2 83.5 3 118.5 4 108.0 5 112.5 6 167.5 7 296.5 8 458.0 9 534.5 10 472.5 11 416.0 12 446.0 13 485.0 14 530.0 15 601.5 16 694.0 17 764.5 18 840.5 19 910.0 20 1054.0 21 1272.0 22 1423.5 23 1619.5 24 1880.5 25 2163.0 26 2559.5 27 3096.0 28 3606.5 29 4037.5 30 4597.5 31 5289.0 32 6187.0 33 6848.0 34 7302.5 35 7954.0 36 8822.0 37 10067.5 38 11576.0 39 13461.5 40 15926.0 41 19559.0 42 24812.0 43 32467.0 44 41594.5 45 51607.5 46 62436.0 47 72027.0 48 80610.5 49 87106.0 50 89921.5 51 91805.5 52 93970.0 53 94407.0 54 92354.0 55 86934.0 56 79214.5 57 71062.0 58 63441.0 59 55486.5 60 46673.5 61 38763.0 62 31448.0 63 24324.0 64 18369.5 65 13817.5 66 10253.0 67 7940.0 68 6113.0 69 4510.0 70 3378.5 71 2476.5 72 1828.0 73 1422.0 74 1043.5 75 726.0 76 504.0 77 292.0 78 164.0 79 88.5 80 51.0 81 32.0 82 18.5 83 11.5 84 6.0 85 4.5 86 3.5 87 1.0 88 1.0 89 1.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.01964491059117717 2 0.0023255657397655218 3 0.0038555432001375757 4 0.035617875277461415 5 0.001591176558786936 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 6.119909841488215E-5 24-25 5.507918857339393E-4 26-27 0.0021725679937283165 28-29 1.529977460372054E-4 30-31 0.006701301276429596 32-33 0.022031675429357574 34-35 0.013861595790970807 36-37 7.037896317711448E-4 38-39 6.731900825637036E-4 40-41 0.002294966190558081 42-43 0.008353676933631413 44-45 0.008292477835216533 46-47 0.008108880539971886 48-49 0.009822455295588586 50-51 0.186687849714598 52-53 0.05250882643996888 54-55 0.017472342597448853 56-57 0.010771041321019258 58-59 0.019216516902272995 60-61 0.05633377009089903 62-63 0.10226369345126807 64-65 0.10654763034030983 66-67 0.11083156722935157 68-69 0.1098829812039209 70-71 0.09837755070192306 72-73 0.07426510592645949 74-75 0.23228117803368523 76-77 0.2794350833623519 78-79 0.007527489105030504 80-81 9.791855746381145E-4 82-83 0.0 84-85 3.059954920744108E-5 86-87 0.0 88-89 2.447963936595286E-4 90-91 0.006425905333562626 92-93 0.01820673177842744 94-95 0.0018665725016539054 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1634011.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 64.92010397688553 #Duplication Level Percentage of deduplicated Percentage of total 1 81.6524841426845 53.00887760514076 2 10.29179860714634 13.362892713702124 3 3.266397818482735 6.3616465801731374 4 1.496925020302256 3.8872211185449577 5 0.8665614165692446 2.8128628633016293 6 0.5298350182871376 2.0638166686677617 7 0.3675218994424687 1.670169194791128 8 0.2790762018202847 1.4494124831717743 9 0.20640857366037504 1.2060059457376968 >10 0.9999583947680841 11.063336302195701 >50 0.03225935580291438 1.3682760280110464 >100 0.010112141792341203 1.0544752186241613 >500 1.8897406899978251E-4 0.09029407129383153 >1k 4.724351724994563E-4 0.6007132066443561 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGT 2881 0.17631460253327547 No Hit ATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTT 2158 0.13206765437931567 RNA PCR Primer, Index 46 (95% over 21bp) GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1941 0.11878745002328625 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1676 0.1025696889433425 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 6.119909841488215E-5 0.0 4 0.0 0.0 0.0 6.119909841488215E-5 0.0 5 0.0 0.0 0.0 6.119909841488215E-5 0.0 6 0.0 0.0 0.0 6.119909841488215E-5 0.0 7 0.0 0.0 0.0 6.119909841488215E-5 0.0 8 0.0 0.0 0.0 6.119909841488215E-5 0.0 9 0.0 0.0 0.0 1.223981968297643E-4 0.0 10-11 0.0 0.0 0.0 1.223981968297643E-4 6.119909841488215E-5 12-13 0.0 0.0 0.0 1.8359729524464646E-4 3.365950412818518E-4 14-15 0.0 0.0 0.0 1.8359729524464646E-4 5.507918857339393E-4 16-17 0.0 0.0 0.0 1.8359729524464646E-4 8.261878286009091E-4 18-19 0.0 0.0 0.0 1.8359729524464646E-4 9.179864762232323E-4 20-21 0.0 0.0 0.0 2.1419684445208754E-4 0.0010097851238455556 22-23 0.0 0.0 0.0 8.261878286009091E-4 0.0011015837714678787 24-25 6.119909841488215E-5 0.0 0.0 0.0018053734032390236 0.001223981968297643 26-27 1.223981968297643E-4 0.0 0.0 0.0038555432001375757 0.0013769797143348484 28-29 1.223981968297643E-4 0.0 0.0 0.00829247783521653 0.0014075792635422895 30-31 1.223981968297643E-4 0.0 0.0 0.025734220883457944 0.0014075792635422895 32-33 1.223981968297643E-4 0.0 0.0 0.06686001501825875 0.0014075792635422895 34-35 1.223981968297643E-4 0.0 0.0 0.1260395431854498 0.0014075792635422895 36-37 1.223981968297643E-4 0.0 0.0 0.21456403904257682 0.0014075792635422895 38-39 1.223981968297643E-4 0.0 0.0 0.34219475878681355 0.0014381788127497306 40-41 1.223981968297643E-4 0.0 0.0 0.470498668613614 0.0014687783619571717 42-43 1.223981968297643E-4 0.0 0.0 0.5962934154054043 0.0014687783619571717 44-45 1.223981968297643E-4 0.0 0.0 0.7473939893917483 0.0015911765587869361 46-47 1.223981968297643E-4 0.0 0.0 0.9136413402357757 0.0017747738540315823 48-49 1.223981968297643E-4 0.0 0.0 1.0864981937086102 0.0018359729524464645 50-51 1.223981968297643E-4 0.0 0.0 1.2584982598036367 0.0018971720508613467 52-53 1.223981968297643E-4 0.0 0.0 1.4281727601588974 0.002141968444520875 54-55 1.223981968297643E-4 0.0 0.0 1.6125656436829372 0.0022031675429357573 56-57 1.223981968297643E-4 0.0 0.0 1.816511639150532 0.0022031675429357573 58-59 1.223981968297643E-4 0.0 0.0 2.0408981334886978 0.0022031675429357573 60-61 1.223981968297643E-4 0.0 0.0 2.269599164265112 0.002356165288972963 62-63 1.223981968297643E-4 0.0 0.0 2.495791032006516 0.0026315612318399324 64-65 1.223981968297643E-4 0.0 0.0 2.733090536110222 0.0026315612318399324 66-67 1.223981968297643E-4 0.0 0.0 2.9999186051991082 0.0026621607810473737 68-69 1.223981968297643E-4 0.0 0.0 3.2869729763141127 0.0026927603302548146 70-71 1.223981968297643E-4 0.0 0.0 3.593519260274258 0.0027233598794622555 72-73 1.223981968297643E-4 0.0 0.0 3.9138353413777507 0.002876357625499461 74-75 1.223981968297643E-4 0.0 0.0 4.239995936379865 0.0029987558223292256 76-77 1.223981968297643E-4 0.0 0.0 4.593451329275018 0.0030599549207441074 78-79 1.223981968297643E-4 0.0 0.0 4.992591849136878 0.0030599549207441074 80-81 1.223981968297643E-4 0.0 0.0 5.409143512497774 0.0030905544699515487 82-83 1.223981968297643E-4 0.0 0.0 5.846319272024484 0.0033047513144036362 84-85 1.223981968297643E-4 0.0 0.0 6.303721333577314 0.0033659504128185185 86-87 1.223981968297643E-4 0.0 0.0 6.782757276419804 0.0034577490604408415 88-89 1.223981968297643E-4 0.0 0.0 7.288690223015634 0.0035495477080631646 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1625 0.0 54.9319 1 GTATCAA 2910 0.0 42.749245 1 ATCAACG 3840 0.0 31.400957 3 CAACGCA 3845 0.0 31.349567 5 TCAACGC 3900 0.0 31.282078 4 TATCAAC 3945 0.0 30.92714 2 AACGCAG 4075 0.0 29.579237 6 GTACATG 5485 0.0 25.450178 1 TACATGG 5560 0.0 24.590727 2 GTGGTAT 715 0.0 24.570606 1 TATCACG 140 1.7877028E-5 23.736883 2 ACGCAGA 5065 0.0 23.610327 7 GTATAAT 250 3.8562575E-10 22.790895 1 ACATGGG 5980 0.0 22.148455 3 CGCAGAG 5465 0.0 21.621712 8 CATGGGG 4605 0.0 20.514008 4 GTATACG 295 3.0340743E-9 19.31432 1 GTATAAG 395 1.8189894E-12 19.232822 1 CTAGTAC 395 1.8189894E-12 19.22929 3 TGGTATC 825 0.0 18.989508 2 >>END_MODULE