FastQCFastQC Report
Sat 18 Jun 2016
SRR1930906_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1930906_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2769601
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN50850.18360045363935096No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA23500.052.6158641
GTATCAA45050.046.3023221
ATCAACG55400.037.3583833
TCAACGC56450.037.3575744
TATCAAC57950.035.5504462
CAACGCA58200.035.512895
AACGCAG62700.033.3892486
ACGCAGA72600.028.7323677
GTACATG52300.027.8705671
CGCAGAG76800.027.414418
TACATGG55250.025.4648082
GCAGAGT80350.024.7495029
ACATGGG57650.024.2240123
GTGGTAT11200.023.7852291
TGGTATC12150.021.091552
AGAGTAC71700.020.13631810-11
GAGTACT52450.019.86587512-13
CATGGGG43850.019.8093324
TATACCG2752.3504981E-819.3163575
CAGAGTA77400.019.31244310-11
Produced by FastQC (version 0.11.5)