##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1930906_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2769601 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 23.063410938976407 30.0 16.0 31.0 2.0 33.0 2 21.998849292732057 30.0 10.0 31.0 2.0 33.0 3 22.445125489195014 30.0 10.0 31.0 2.0 33.0 4 24.44592163275504 33.0 10.0 35.0 2.0 37.0 5 24.418444389643128 32.0 10.0 35.0 2.0 37.0 6 25.141854007129545 32.0 17.0 35.0 2.0 37.0 7 25.212952335011433 32.0 17.0 35.0 2.0 37.0 8 25.95050261752505 33.0 17.0 35.0 2.0 37.0 9 27.11505700640634 34.0 17.0 37.0 2.0 39.0 10-11 27.33991358322011 33.5 18.5 37.0 2.0 39.0 12-13 27.257421917453094 34.0 18.0 37.0 2.0 39.0 14-15 27.485609118425362 34.0 17.0 38.0 2.0 40.0 16-17 26.620297291920387 33.0 14.0 38.0 2.0 40.0 18-19 26.015864017957824 32.5 10.0 38.0 2.0 40.0 20-21 25.512175219463018 32.0 10.0 38.0 2.0 40.0 22-23 25.432279053914264 32.0 10.0 38.0 2.0 40.0 24-25 25.127486594639443 32.0 10.0 38.0 2.0 40.0 26-27 24.78919526675503 31.5 9.0 38.0 2.0 40.0 28-29 24.70539781723071 31.0 8.5 38.0 2.0 40.0 30-31 24.600112434968068 31.0 8.0 37.5 2.0 39.0 32-33 24.286718014616547 31.0 5.0 37.0 2.0 39.0 34-35 21.365487122513315 25.5 2.0 36.0 2.0 39.0 36-37 19.752239040930444 18.5 2.0 35.0 2.0 38.5 38-39 18.80740077722387 18.5 2.0 34.0 2.0 38.0 40-41 20.100368067458092 23.5 2.0 35.0 2.0 38.0 42-43 20.008759023411677 23.5 2.0 35.0 2.0 38.0 44-45 18.057934157302803 18.0 2.0 34.0 2.0 38.0 46-47 18.879916276748887 19.0 2.0 33.0 2.0 38.0 48-49 18.270966648264498 18.5 2.0 33.0 2.0 38.0 50-51 16.31230653801757 11.0 2.0 31.5 2.0 37.5 52-53 19.199752238679867 22.0 2.0 33.5 2.0 37.5 54-55 20.818432510675727 26.5 2.0 35.5 2.0 38.5 56-57 21.02234112422692 28.0 2.0 36.0 2.0 39.0 58-59 20.644664339737023 26.5 2.0 36.0 2.0 39.0 60-61 20.316199878610675 26.0 2.0 35.0 2.0 39.0 62-63 19.827782593954872 25.5 2.0 35.0 2.0 38.0 64-65 19.320199913272706 24.5 2.0 34.0 2.0 38.0 66-67 18.886324781078574 23.0 2.0 34.0 2.0 38.0 68-69 18.410725407739235 22.0 2.0 34.0 2.0 37.0 70-71 17.818920126039814 20.5 2.0 33.0 2.0 36.5 72-73 17.334681241088518 18.5 2.0 32.5 2.0 36.0 74-75 16.78475112480101 14.5 2.0 32.0 2.0 35.5 76-77 16.210457571325257 9.5 2.0 31.5 2.0 35.0 78-79 15.705381027808698 7.0 2.0 31.0 2.0 35.0 80-81 15.188528058734814 2.0 2.0 31.0 2.0 34.0 82-83 14.695768090782753 2.0 2.0 30.5 2.0 34.0 84-85 14.162336740924054 2.0 2.0 30.0 2.0 34.0 86-87 13.595630200884532 2.0 2.0 29.5 2.0 34.0 88-89 13.04147637150622 2.0 2.0 29.0 2.0 34.0 90-91 12.457323094554052 2.0 2.0 29.0 2.0 34.0 92-93 11.82596012927494 2.0 2.0 27.5 2.0 34.0 94-95 11.155677658984093 2.0 2.0 26.0 2.0 33.0 96-97 10.431519377700976 2.0 2.0 25.0 2.0 33.0 98-99 9.6345509696162 2.0 2.0 22.0 2.0 32.0 100-101 8.596282280371794 2.0 2.0 8.5 2.0 31.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 6.665061249849675 1101 2 7.855226227497116 1101 3 7.434708038320043 1101 4 9.281704123329892 1101 5 9.186726107808951 1101 6 8.531676764528509 1101 7 8.18161323390957 1101 8 7.465756076165842 1101 9 7.547533638248691 1101 10-11 6.820053543705484 1101 12-13 6.767192021468031 1101 14-15 7.236945652741635 1101 16-17 7.860626492874541 1101 18-19 8.32395842512376 1101 20-21 8.903851558987636 1101 22-23 8.85077582885302 1101 24-25 8.735356452181751 1101 26-27 8.68125175726102 1101 28-29 8.5739784191239 1101 30-31 8.49045741157989 1101 32-33 8.439122472434534 1101 34-35 10.92520899445491 1101 36-37 5.291618586481999 1101 38-39 8.492512606060469 1101 40-41 9.725185609101278 1101 42-43 10.237063179646817 1101 44-45 4.464341427421626 1101 46-47 6.10494719317035 1101 48-49 9.175379511863763 1101 50-51 -3.2780608811487255 1101 52-53 5.96340513046027 1101 54-55 8.331172602516492 1101 56-57 9.018167411949602 1101 58-59 9.239470522974916 1101 60-61 8.907706828608635 1101 62-63 9.0704290481929 1101 64-65 9.069124059718053 1101 66-67 8.959426545505988 1101 68-69 8.99320933545464 1101 70-71 8.88140088180911 1101 72-73 8.713116676575389 1101 74-75 8.885485777833946 1101 76-77 8.667515063118053 1101 78-79 8.403053821657457 1101 80-81 8.446282582000315 1101 82-83 8.083687455357055 1101 84-85 8.079249805581656 1101 86-87 7.922466307670248 1101 88-89 7.810604954901455 1101 90-91 7.5204800036603725 1101 92-93 7.3446711046136155 1101 94-95 7.167730796229478 1101 96-97 6.985838835533256 1101 98-99 6.617573520583406 1101 100-101 6.365482170087461 1102 1 6.524866755414912 1102 2 7.448948203764363 1102 3 7.2436482724398275 1102 4 8.827037884365428 1102 5 8.959573543128819 1102 6 8.180328441176204 1102 7 8.00742820710349 1102 8 7.075217572353079 1102 9 7.018742282236136 1102 10-11 6.707235214149925 1102 12-13 6.481866138329803 1102 14-15 6.8934568292136085 1102 16-17 7.728877151528085 1102 18-19 7.877331533190112 1102 20-21 8.307597687850222 1102 22-23 8.023111436712984 1102 24-25 8.234257910064482 1102 26-27 8.178258038351 1102 28-29 8.040156648295234 1102 30-31 7.969848926565589 1102 32-33 7.9575676019363115 1102 34-35 10.030967643041723 1102 36-37 0.9395618420348555 1102 38-39 3.185862269127046 1102 40-41 7.9775380105626965 1102 42-43 9.094593765465191 1102 44-45 0.9697083039296892 1102 46-47 1.5636482599885966 1102 48-49 7.09815901800923 1102 50-51 -4.579128171128465 1102 52-53 4.363065720453452 1102 54-55 7.115854455756452 1102 56-57 7.676178219292556 1102 58-59 7.703619014263236 1102 60-61 7.795906375275742 1102 62-63 7.897374405304589 1102 64-65 7.876351912566481 1102 66-67 7.7503554062063635 1102 68-69 7.64468302115484 1102 70-71 7.593108286324988 1102 72-73 7.3151809687085745 1102 74-75 7.2616125648075815 1102 76-77 7.335083599645316 1102 78-79 7.229858603116567 1102 80-81 7.018713243531408 1102 82-83 6.82579210402406 1102 84-85 6.845691024438606 1102 86-87 6.546640956408016 1102 88-89 6.502036562251957 1102 90-91 6.305804117809691 1102 92-93 6.3523200947568075 1102 94-95 6.016019712216654 1102 96-97 5.7150352900077355 1102 98-99 5.487123914360341 1102 100-101 5.107944862111207 1103 1 6.621113650077213 1103 2 7.5553759625811985 1103 3 7.2370603130239815 1103 4 8.867400309198846 1103 5 8.966604956376713 1103 6 8.300339585565705 1103 7 7.963844357181269 1103 8 7.25225664736875 1103 9 7.253897026727788 1103 10-11 6.669017503857617 1103 12-13 6.659801408000799 1103 14-15 6.887064477050895 1103 16-17 7.723408274557503 1103 18-19 8.135906908732824 1103 20-21 8.637032806607618 1103 22-23 8.542625727070774 1103 24-25 8.529280663193045 1103 26-27 8.419625779986905 1103 28-29 8.434470687904263 1103 30-31 8.148303106872518 1103 32-33 8.099648120150423 1103 34-35 10.413740274278517 1103 36-37 -2.9152900304547558 1103 38-39 -1.5480706555786732 1103 40-41 6.866134746185274 1103 42-43 9.185173273719002 1103 44-45 1.0551137971850117 1103 46-47 1.0164053355150529 1103 48-49 7.574729680403884 1103 50-51 -4.778475179556409 1103 52-53 4.636064373839719 1103 54-55 7.528946791666929 1103 56-57 8.145725014119702 1103 58-59 8.360615020856997 1103 60-61 8.324718685182638 1103 62-63 8.308096236699264 1103 64-65 8.223093919949637 1103 66-67 7.938256778359321 1103 68-69 8.02834372092963 1103 70-71 7.76940091941918 1103 72-73 7.776648661587778 1103 74-75 7.835668774322102 1103 76-77 7.772568798503016 1103 78-79 7.594529147102078 1103 80-81 7.1803359130770446 1103 82-83 7.065583378895788 1103 84-85 6.921237215611322 1103 86-87 6.657701182546251 1103 88-89 6.510285151790162 1103 90-91 6.305447729520585 1103 92-93 6.385184435032167 1103 94-95 6.039295234029474 1103 96-97 5.983024192053195 1103 98-99 5.593565603664048 1103 100-101 5.160702518074618 1104 1 6.16901681134172 1104 2 7.45602022028428 1104 3 6.906452069726662 1104 4 8.658800869422937 1104 5 8.572019122042974 1104 6 7.786630101772186 1104 7 7.561507422407356 1104 8 6.613613189985799 1104 9 7.0068422047990175 1104 10-11 6.484757799976066 1104 12-13 6.076852228328942 1104 14-15 6.524189326990875 1104 16-17 7.15775001125218 1104 18-19 7.7437020267800385 1104 20-21 8.047152854626827 1104 22-23 7.7439262472788535 1104 24-25 7.547838086162226 1104 26-27 7.723231222163772 1104 28-29 7.4120977387245865 1104 30-31 7.292688861174241 1104 32-33 7.197139116548982 1104 34-35 9.584356520777117 1104 36-37 -3.387981106885329 1104 38-39 -2.1454596111609057 1104 40-41 5.69044046847419 1104 42-43 7.825725494607358 1104 44-45 0.22875525376764472 1104 46-47 -0.2736706948971097 1104 48-49 6.373967375481911 1104 50-51 -5.195299909448366 1104 52-53 3.6803000681174325 1104 54-55 6.385841549570088 1104 56-57 6.915456906876802 1104 58-59 6.886999574678523 1104 60-61 6.936977588744064 1104 62-63 6.976987793321911 1104 64-65 6.958418049601502 1104 66-67 6.913022684721863 1104 68-69 6.868659847380211 1104 70-71 6.635243256353952 1104 72-73 6.531052423092376 1104 74-75 6.435986901573003 1104 76-77 6.502874920951996 1104 78-79 6.2261077785546615 1104 80-81 6.0573747286032535 1104 82-83 5.741989941520835 1104 84-85 5.8538342544268485 1104 86-87 5.727170970461419 1104 88-89 5.518466424299165 1104 90-91 5.498084784342515 1104 92-93 5.196705043275463 1104 94-95 4.838098755438036 1104 96-97 4.4609153485157815 1104 98-99 4.3108965360369975 1104 100-101 3.838275547286301 1105 1 5.7844167490946 1105 2 6.927598184803422 1105 3 6.547528944921183 1105 4 8.308742300540839 1105 5 8.333543044743372 1105 6 7.3124264405097 1105 7 7.386032222226273 1105 8 6.273863876118835 1105 9 6.305415613960658 1105 10-11 5.819208186191162 1105 12-13 5.670087946858768 1105 14-15 5.814947044219178 1105 16-17 6.600151901301128 1105 18-19 7.1867074309619206 1105 20-21 7.483744824748211 1105 22-23 7.245497987085887 1105 24-25 7.370947329859707 1105 26-27 7.306367345299897 1105 28-29 7.181783956646097 1105 30-31 6.9306641320906905 1105 32-33 6.649778677423839 1105 34-35 8.81595287649112 1105 36-37 -3.9338583668802354 1105 38-39 -2.5343569882935775 1105 40-41 5.01369882232078 1105 42-43 7.164363131480286 1105 44-45 -3.5318510897798756 1105 46-47 -4.50152911099082 1105 48-49 4.350337802440578 1105 50-51 -9.61071767251319 1105 52-53 0.12433311567989236 1105 54-55 4.581331179765602 1105 56-57 5.58977091530846 1105 58-59 6.048155390540224 1105 60-61 5.813526834705073 1105 62-63 5.873330209076091 1105 64-65 5.963956628669489 1105 66-67 5.746220772037599 1105 68-69 5.548672862687344 1105 70-71 5.7598427527181535 1105 72-73 5.646110284620825 1105 74-75 5.503330081269123 1105 76-77 5.390917248994324 1105 78-79 5.220607962466607 1105 80-81 5.285946440116142 1105 82-83 5.105908397792234 1105 84-85 5.058123081068617 1105 86-87 4.915855858558071 1105 88-89 4.755068014430167 1105 90-91 4.758096667883811 1105 92-93 4.531776203052507 1105 94-95 4.328215044377856 1105 96-97 4.260338147938581 1105 98-99 3.8067381454080174 1105 100-101 3.4286283752861895 1106 1 5.790766306888859 1106 2 7.0488388209892605 1106 3 6.587691558547764 1106 4 7.992992977352124 1106 5 7.822624381748984 1106 6 7.08200409461077 1106 7 6.970328970289213 1106 8 6.18432037289217 1106 9 6.355266721068492 1106 10-11 5.799441606411293 1106 12-13 5.702673349205053 1106 14-15 6.135692805831763 1106 16-17 6.547081789131052 1106 18-19 6.860199016808206 1106 20-21 7.271934425840502 1106 22-23 6.996430370814686 1106 24-25 7.0400439754923845 1106 26-27 7.132967901956913 1106 28-29 6.9571757579761275 1106 30-31 6.81717800322761 1106 32-33 6.945148292986083 1106 34-35 9.261587849044059 1106 36-37 -0.008393153470816372 1106 38-39 0.8885760954498672 1106 40-41 6.5133044210960875 1106 42-43 7.726380683285285 1106 44-45 -3.44204977764379 1106 46-47 -4.2686476561233135 1106 48-49 4.785988228991034 1106 50-51 -9.454883620714341 1106 52-53 0.39242139356557715 1106 54-55 4.930892943757787 1106 56-57 5.858223178446874 1106 58-59 6.31232140583543 1106 60-61 6.3206942690395636 1106 62-63 6.496852881834897 1106 64-65 6.4167916879974065 1106 66-67 6.268084823825266 1106 68-69 5.823260206288303 1106 70-71 5.936526673739504 1106 72-73 5.87233528230049 1106 74-75 5.814510131522571 1106 76-77 5.614433491906119 1106 78-79 5.55769464910494 1106 80-81 5.378936509077647 1106 82-83 5.07233458818056 1106 84-85 4.874354922341974 1106 86-87 4.693415524742779 1106 88-89 4.645139156494002 1106 90-91 4.474107993360429 1106 92-93 4.122058188186701 1106 94-95 3.9395113350829796 1106 96-97 3.692879100472892 1106 98-99 3.4617842893448962 1106 100-101 3.0308677512220115 1107 1 5.83355082833835 1107 2 6.951138674445605 1107 3 6.519882255360237 1107 4 7.680795836901453 1107 5 8.131760849242333 1107 6 6.659683323060701 1107 7 6.947780982678925 1107 8 6.085656108848259 1107 9 6.292057036494409 1107 10-11 5.738011372591551 1107 12-13 5.33637746215128 1107 14-15 5.830290072373774 1107 16-17 6.675247993495926 1107 18-19 6.941629197648393 1107 20-21 7.174666504154441 1107 22-23 7.20848495211672 1107 24-25 7.284656203239567 1107 26-27 7.358818745308625 1107 28-29 7.126693000558131 1107 30-31 7.0308913964256305 1107 32-33 6.6399744201619555 1107 34-35 8.940315311072897 1107 36-37 -0.38992954727843454 1107 38-39 0.8294062673939067 1107 40-41 6.605121188219716 1107 42-43 7.535288234957733 1107 44-45 -3.4303322456388976 1107 46-47 -4.276253422903567 1107 48-49 4.4693222631997145 1107 50-51 -9.521761477126287 1107 52-53 0.2482324614137319 1107 54-55 4.66530031612756 1107 56-57 5.787458995847988 1107 58-59 6.0292385719723605 1107 60-61 6.108201061183884 1107 62-63 6.09389326737594 1107 64-65 5.97671079042664 1107 66-67 5.827622694217187 1107 68-69 5.733318253115385 1107 70-71 5.750892499101898 1107 72-73 5.5623888898824845 1107 74-75 5.573785885573383 1107 76-77 5.636240538385408 1107 78-79 5.564018807372966 1107 80-81 5.490497265753703 1107 82-83 5.269940375931888 1107 84-85 4.98972088026362 1107 86-87 4.966160668035364 1107 88-89 4.8513658213461195 1107 90-91 4.670534103053049 1107 92-93 4.497636534516028 1107 94-95 4.289282347518938 1107 96-97 4.033549826035603 1107 98-99 3.867088911292184 1107 100-101 3.6301447017277404 1108 1 6.15281806179971 1108 2 6.333013093382888 1108 3 6.670782578301399 1108 4 7.8539283381319365 1108 5 8.138201443072656 1108 6 7.58420967368534 1108 7 7.5513009431911975 1108 8 6.485816738071698 1108 9 5.0305381473110415 1108 10-11 5.877873957831611 1108 12-13 5.3110098238565975 1108 14-15 6.01688307115521 1108 16-17 6.133122962120293 1108 18-19 6.672155796473579 1108 20-21 7.582689972427541 1108 22-23 7.581269201338113 1108 24-25 7.188141422686709 1108 26-27 7.466077314187082 1108 28-29 7.432021564795097 1108 30-31 7.376622662542893 1108 32-33 7.365104935788072 1108 34-35 9.620340635942167 1108 36-37 -3.4267620876320564 1108 38-39 -2.0687931217803417 1108 40-41 5.605815133107974 1108 42-43 7.848035296165111 1108 44-45 -3.019564724012497 1108 46-47 -3.8941611273570125 1108 48-49 5.255819837985904 1108 50-51 -9.51418803009747 1108 52-53 0.6360270593358592 1108 54-55 5.4038901023245245 1108 56-57 6.649588500442501 1108 58-59 7.070677020340334 1108 60-61 7.044503265681506 1108 62-63 6.997307321977253 1108 64-65 6.7888958947479985 1108 66-67 6.761396793859188 1108 68-69 6.69208909249598 1108 70-71 6.648951136130339 1108 72-73 6.555880987429504 1108 74-75 6.46497501158494 1108 76-77 6.381681430897746 1108 78-79 6.377386720511188 1108 80-81 6.287008750988576 1108 82-83 6.123583034454214 1108 84-85 5.889428753829984 1108 86-87 5.977391041612243 1108 88-89 5.739815276076163 1108 90-91 5.796841713274427 1108 92-93 5.730399567498656 1108 94-95 5.346153926873701 1108 96-97 5.345228187575456 1108 98-99 5.239524873447911 1108 100-101 4.953330033215694 1109 1 4.739397122962696 1109 2 6.423284322147481 1109 3 5.969327553603318 1109 4 7.843818137610377 1109 5 7.667522574210832 1109 6 4.975672048036525 1109 7 4.810705326122882 1109 8 2.9912673618329215 1109 9 2.9942861309529007 1109 10-11 2.643054201432765 1109 12-13 3.3835027878182125 1109 14-15 3.76048514647292 1109 16-17 3.093622635223067 1109 18-19 3.912063121480255 1109 20-21 4.4020087865064035 1109 22-23 6.063932948805213 1109 24-25 5.9384009849838755 1109 26-27 4.7198774021467855 1109 28-29 4.589698004434229 1109 30-31 4.714636418791201 1109 32-33 4.265406448665459 1109 34-35 6.720703248345377 1109 36-37 -4.524926445356499 1109 38-39 -3.035066072843165 1109 40-41 3.4741388292455717 1109 42-43 4.091793256444518 1109 44-45 -4.424172166938309 1109 46-47 -5.708947470875078 1109 48-49 2.0789439054433174 1109 50-51 -9.760023780108908 1109 52-53 -1.0210804463128937 1109 54-55 2.413829475065576 1109 56-57 3.9108162762279015 1109 58-59 4.759325680356891 1109 60-61 4.837379562755075 1109 62-63 4.933223475422665 1109 64-65 4.478890330853979 1109 66-67 4.656807577482748 1109 68-69 4.636206824198222 1109 70-71 4.7533977500506985 1109 72-73 4.598889740600704 1109 74-75 4.62343511905517 1109 76-77 4.867991595113789 1109 78-79 4.868199654129652 1109 80-81 4.552129961231458 1109 82-83 4.4671516452369495 1109 84-85 4.251531809839358 1109 86-87 4.290984726813907 1109 88-89 4.464040389457109 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74-75 -14.144896956759261 2313 76-77 -13.635381806357588 2313 78-79 -13.152194796111246 2313 80-81 -12.606027514876615 2313 82-83 -12.106736616857038 2313 84-85 -11.568861796678945 2313 86-87 -11.026962952706997 2313 88-89 -10.499252714438274 2313 90-91 -9.922865490056 2313 92-93 -9.316017926011575 2313 94-95 -8.790976874814064 2313 96-97 -8.065720236544777 2313 98-99 -7.240445467951544 2313 100-101 -6.233219857544662 2314 1 -20.578546213868364 2314 2 -19.461290704085467 2314 3 -19.893211795819557 2314 4 -21.806409214610678 2314 5 -21.773190961990636 2314 6 -22.536395108311847 2314 7 -22.606223670029618 2314 8 -23.375294373039413 2314 9 -24.54576283721779 2314 10-11 -24.778771611788642 2314 12-13 -24.705164578393752 2314 14-15 -24.88589470662257 2314 16-17 -24.02897267782345 2314 18-19 -23.40150805725357 2314 20-21 -22.88847739747401 2314 22-23 -22.789687198099298 2314 24-25 -22.479052670140295 2314 26-27 -22.15962592069337 2314 28-29 -22.081515706653565 2314 30-31 -21.964622063195506 2314 32-33 -21.670760043114882 2314 34-35 -18.88387877334053 2314 36-37 -17.236888669910538 2314 38-39 -16.340417594354182 2314 40-41 -17.62502171639976 2314 42-43 -17.52503080791365 2314 44-45 -15.571076679005465 2314 46-47 -16.325771535233244 2314 48-49 -15.790066237963465 2314 50-51 -13.837148648944165 2314 52-53 -16.67533018398341 2314 54-55 -18.234998786564365 2314 56-57 -18.427064101526557 2314 58-59 -18.039555047170214 2314 60-61 -17.733274512096273 2314 62-63 -17.250407164661283 2314 64-65 -16.731497916785056 2314 66-67 -16.306641180824354 2314 68-69 -15.859071245056764 2314 70-71 -15.276857583982181 2314 72-73 -14.813317324806782 2314 74-75 -14.265861837922795 2314 76-77 -13.694267936190862 2314 78-79 -13.207001465142818 2314 80-81 -12.71422191005221 2314 82-83 -12.225573083689248 2314 84-85 -11.693728770783236 2314 86-87 -11.151652558950346 2314 88-89 -10.622878113515961 2314 90-91 -10.048974039186897 2314 92-93 -9.43556386428756 2314 94-95 -8.790976874814064 2314 96-97 -8.096227508627075 2314 98-99 -7.319019430349558 2314 100-101 -6.331304284646471 2315 1 -20.578546213868364 2315 2 -19.461290704085467 2315 3 -19.893211795819557 2315 4 -16.61648532914493 2315 5 -21.773190961990636 2315 6 -22.536395108311847 2315 7 -22.606223670029618 2315 8 -23.375294373039413 2315 9 -19.72735036893218 2315 10-11 -17.955103616138043 2315 12-13 -14.539162403692774 2315 14-15 -14.485568501475775 2315 16-17 -19.8178454578162 2315 18-19 -23.40150805725357 2315 20-21 -22.88847739747401 2315 22-23 -22.789687198099298 2315 24-25 -22.479052670140295 2315 26-27 -22.15962592069337 2315 28-29 -22.081515706653565 2315 30-31 -21.964622063195506 2315 32-33 -21.670760043114882 2315 34-35 -18.88387877334053 2315 36-37 -17.236888669910538 2315 38-39 -16.340417594354182 2315 40-41 -17.62502171639976 2315 42-43 -17.52503080791365 2315 44-45 -15.571076679005465 2315 46-47 -16.325771535233244 2315 48-49 -15.790066237963465 2315 50-51 -13.837148648944165 2315 52-53 -16.67533018398341 2315 54-55 -18.234998786564365 2315 56-57 -18.427064101526557 2315 58-59 -18.039555047170214 2315 60-61 -17.733274512096273 2315 62-63 -17.250407164661283 2315 64-65 -16.731497916785056 2315 66-67 -16.306641180824354 2315 68-69 -15.859071245056764 2315 70-71 -15.276857583982181 2315 72-73 -14.813317324806782 2315 74-75 -14.265861837922795 2315 76-77 -13.694267936190862 2315 78-79 -13.207001465142818 2315 80-81 -12.71422191005221 2315 82-83 -12.225573083689248 2315 84-85 -11.693728770783236 2315 86-87 -11.151652558950346 2315 88-89 -10.622878113515961 2315 90-91 -10.048974039186897 2315 92-93 -9.43556386428756 2315 94-95 -8.790976874814064 2315 96-97 -8.096227508627075 2315 98-99 -7.319019430349558 2315 100-101 -6.331304284646471 2316 1 -20.578546213868364 2316 2 -19.461290704085467 2316 3 -19.893211795819557 2316 4 -21.806409214610678 2316 5 -21.773190961990636 2316 6 -22.536395108311847 2316 7 -22.606223670029618 2316 8 -23.375294373039413 2316 9 -24.54576283721779 2316 10-11 -24.778771611788642 2316 12-13 -24.705164578393752 2316 14-15 -24.88589470662257 2316 16-17 -24.02897267782345 2316 18-19 -23.40150805725357 2316 20-21 -22.88847739747401 2316 22-23 -22.789687198099298 2316 24-25 -22.479052670140295 2316 26-27 -22.15962592069337 2316 28-29 -22.081515706653565 2316 30-31 -21.964622063195506 2316 32-33 -21.670760043114882 2316 34-35 -18.88387877334053 2316 36-37 -17.236888669910538 2316 38-39 -16.340417594354182 2316 40-41 -17.62502171639976 2316 42-43 -17.52503080791365 2316 44-45 -15.571076679005465 2316 46-47 -16.325771535233244 2316 48-49 -15.790066237963465 2316 50-51 -13.837148648944165 2316 52-53 -16.67533018398341 2316 54-55 -18.234998786564365 2316 56-57 -18.427064101526557 2316 58-59 -18.039555047170214 2316 60-61 -17.733274512096273 2316 62-63 -17.250407164661283 2316 64-65 -16.731497916785056 2316 66-67 -16.306641180824354 2316 68-69 -15.859071245056764 2316 70-71 -15.276857583982181 2316 72-73 -14.813317324806782 2316 74-75 -14.265861837922795 2316 76-77 -13.694267936190862 2316 78-79 -13.207001465142818 2316 80-81 -12.71422191005221 2316 82-83 -12.225573083689248 2316 84-85 -11.693728770783236 2316 86-87 -11.151652558950346 2316 88-89 -10.622878113515961 2316 90-91 -10.048974039186897 2316 92-93 -9.43556386428756 2316 94-95 -8.790976874814064 2316 96-97 -8.096227508627075 2316 98-99 -7.319019430349558 2316 100-101 -6.331304284646471 >>END_MODULE >>Per sequence quality scores fail #Quality Count 2 438173.0 3 28433.0 4 58436.0 5 35769.0 6 31867.0 7 39462.0 8 50335.0 9 58964.0 10 61505.0 11 57779.0 12 51780.0 13 47594.0 14 45607.0 15 48561.0 16 52777.0 17 55659.0 18 57361.0 19 60049.0 20 63341.0 21 66469.0 22 70573.0 23 74760.0 24 78649.0 25 83107.0 26 88363.0 27 93303.0 28 98805.0 29 104757.0 30 107900.0 31 107486.0 32 104014.0 33 100699.0 34 93172.0 35 77283.0 36 52922.0 37 21343.0 38 2530.0 39 14.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.22872205912153 19.96817164035377 13.921118254791 23.881988045733692 2 15.456943479662394 20.545544522989054 40.51253762649995 23.484974370848597 3 18.632882993573844 25.530160175410916 30.09973348646437 25.73722334455087 4 12.625563066943233 16.860102127307684 38.48191868865413 32.032416117094954 5 13.295102597875175 37.48697304416929 35.45229315668919 13.765631201266354 6 30.969076822106494 37.2678417701559 17.929945534976092 13.83313587276152 7 26.930439312293014 32.49866618345159 22.818037992644985 17.752856511610403 8 25.327624919409786 35.70923255311012 20.789932729828216 18.173209797651875 9 26.02906545282522 16.14244873857332 20.97603792845753 36.852447880143934 10-11 24.715517034174106 26.286324108391863 28.841349273878368 20.156809583555667 12-13 25.179886748244833 24.02374473328541 28.83131367516531 21.965054843304443 14-15 22.39590851590572 25.42822053037182 26.533355839600166 25.642515114122293 16-17 22.16910418846641 28.406044589722075 27.197879850071754 22.226971371739758 18-19 22.050696928838576 27.454937436201597 29.096197835053406 21.398167799906425 20-21 22.771621457000517 26.664442073508777 29.138285044863917 21.425651424626793 22-23 22.52716374520747 26.4952825216487 28.88933317908747 22.088220554056363 24-25 22.351533726122224 26.810282966524873 28.78729689027451 22.05088641707839 26-27 22.25612478483989 26.935000763322027 28.79182788672548 22.0170465651126 28-29 22.249879921792317 27.108300772511708 28.782545822564003 21.859273483131968 30-31 22.455573908022455 26.89944919076414 28.644840745188567 22.00013615602484 32-33 22.388649305025325 27.007591192713893 28.509742547834886 22.094016954425896 34-35 22.690023012180266 26.950925928383544 28.518293400432288 21.840757659003902 36-37 23.173839964569012 26.869428886336443 28.068460208014034 21.88827094108051 38-39 22.95344411837565 26.980404018099446 28.102318284170963 21.96383357935394 40-41 22.888798636997606 26.929532453253728 28.275010655276017 21.906658254472653 42-43 22.9994636716546 26.89105865897864 27.968923902177718 22.14055376718904 44-45 23.250236346041326 27.14299878091525 27.647389859951875 21.959375013091545 46-47 22.64496247846503 27.107534100972856 27.949932163297262 22.29757125726485 48-49 23.177448899723665 27.200632358947484 27.434147109967533 22.187771631361326 50-51 23.373204788378068 27.313071141953365 27.2251839924945 22.088540077174063 52-53 22.456533006025854 27.421664793485 27.722261528439578 22.399540672049568 54-55 22.294265424511657 27.31779551496336 27.898190041997562 22.48974901852742 56-57 22.315613935416838 27.51656279649356 27.70143795419801 22.466385313891593 58-59 22.35650825571768 27.383091315649832 27.85043699453473 22.409963434097754 60-61 22.334938218190075 27.490592354938247 27.81392854600468 22.360540880866996 62-63 22.23223032758012 27.39443749823155 27.877895282457533 22.4954368917308 64-65 22.157439423391363 27.237147234731975 27.84274732370659 22.762666018170076 66-67 22.207604854613074 27.377537691869918 27.674470929232236 22.740386524284773 68-69 22.27160722652127 27.344732249046228 27.72908268075313 22.65457784367937 70-71 22.19161116862805 27.228254791760833 27.589363292565878 22.990770747045243 72-73 22.365535230151597 27.28683970231146 27.484457686152574 22.86316738138437 74-75 22.319531912884862 27.408470320712457 27.43780242427617 22.83419534212651 76-77 22.356066329144795 27.30061101983745 27.44612846477077 22.897194186246985 78-79 22.287796363500707 27.366442417431223 27.359062285274145 22.986698933793928 80-81 22.371045466233916 27.43716890408125 27.28095617026114 22.910829459423695 82-83 22.465387776598547 27.31638564600174 27.356444057869243 22.861782519530472 84-85 22.43395755325769 27.37904348271398 27.178165806080212 23.008833157948118 86-87 22.288760336648618 27.43918162643244 27.191513210312777 23.08054482660616 88-89 22.381347938222117 27.283477318205048 27.08503604533706 23.250138698235784 90-91 22.39118598595439 27.310897182987453 27.086423893316496 23.211492937741657 92-93 22.48269816881187 27.159718898768293 27.137492758368808 23.22009017405103 94-95 22.279364335076114 27.121363108747946 27.149448808062694 23.449823748113243 96-97 22.390700192758562 26.976016240079403 27.07169260813699 23.56159095902505 98-99 22.240618289597354 27.067427889698692 26.96877062667406 23.723183194029893 100-101 22.641573822460586 27.10520193200795 26.813365229678375 23.439859015853088 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 30673.0 1 32888.0 2 24409.5 3 14493.5 4 18723.0 5 23964.5 6 25606.5 7 24500.0 8 22582.0 9 20631.0 10 18502.0 11 16224.0 12 14089.0 13 12141.0 14 10379.5 15 9031.0 16 8111.5 17 7540.5 18 7383.5 19 7549.5 20 8064.0 21 9014.5 22 10365.5 23 12128.5 24 14322.5 25 17073.5 26 20426.5 27 24299.5 28 28594.0 29 33501.0 30 38764.0 31 44017.0 32 49484.5 33 54555.5 34 59053.0 35 63568.0 36 68446.5 37 73168.5 38 76674.5 39 79084.0 40 80906.0 41 82453.0 42 83986.0 43 85903.5 44 87280.5 45 87892.5 46 88237.5 47 88580.0 48 87955.0 49 86302.0 50 84127.5 51 81129.5 52 77830.5 53 74118.5 54 69535.5 55 63537.0 56 56978.5 57 50238.0 58 43723.5 59 37794.0 60 31892.0 61 26467.5 62 21713.0 63 17259.0 64 13357.0 65 10218.5 66 7831.5 67 5977.0 68 4531.5 69 3424.5 70 2612.0 71 1986.5 72 1426.5 73 1053.0 74 812.5 75 586.0 76 414.5 77 303.5 78 206.5 79 128.0 80 76.5 81 42.0 82 27.5 83 19.5 84 9.5 85 6.5 86 7.0 87 4.5 88 1.5 89 2.0 90 2.0 91 1.0 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.5 100 0.5 >>END_MODULE >>Per base N content fail #Base N-Count 1 13.762451703331996 2 20.473382266976362 3 16.248513775088902 4 21.407632362928812 5 21.2155830388565 6 15.278049076383205 7 14.120734358487017 8 11.347410692009428 9 2.8393981660174155 10-11 6.11328129936406 12-13 3.332303100699342 14-15 6.951940008687171 16-17 10.837409431900118 18-19 13.79974949460229 20-21 14.194698081059329 22-23 12.14097265273951 24-25 14.588328788153962 26-27 12.255700369836665 28-29 12.236184923387881 30-31 13.019925974896745 32-33 11.57015035739805 34-35 18.380228776636056 36-37 22.999865323561046 38-39 22.16763353277241 40-41 17.2762249869205 42-43 17.16122647269408 44-45 22.4316787869444 46-47 12.018590403455228 48-49 20.702621063467262 50-51 20.258766515465584 52-53 10.514059606419842 54-55 12.063199717215586 56-57 11.5970495389047 58-59 12.563037058406609 60-61 12.000085932955686 62-63 11.951974309656878 64-65 12.995554233263205 66-67 11.337318985658944 68-69 12.338907301087774 70-71 11.051248898307014 72-73 10.418522379216357 74-75 10.510105968332622 76-77 10.576324893008055 78-79 10.958997342938567 80-81 10.908881820883225 82-83 9.505376406204359 84-85 9.400722342315735 86-87 9.547837396072575 88-89 10.87205341130365 90-91 8.485012823146727 92-93 10.409026426550252 94-95 10.523952728208865 96-97 10.108243028508438 98-99 9.933073392160098 100-101 10.067316555706038 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2769601.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 97.65428584931398 #Duplication Level Percentage of deduplicated Percentage of total 1 99.13971325424492 96.81417897149056 2 0.6008429875115653 1.1734978570602037 3 0.11946622189483137 0.3499916574676631 4 0.04159471234172468 0.1624760771535508 5 0.02688628249868847 0.13127803582761657 6 0.01594859372250166 0.09344691181630511 7 0.011488554672221018 0.07853346213695801 8 0.007926073128184417 0.061921200873782986 9 0.005345119877002119 0.04697764779308535 >10 0.02751113347830978 0.4402653784744156 >50 0.0020116985355895438 0.13845009760292723 >100 0.0011173803363258609 0.16452789078711902 >500 3.6996939520960775E-5 0.035984580686995664 >1k 7.399387904192155E-5 0.12475377220100001 >5k 3.6996939520960775E-5 0.18371645862788447 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 5085 0.18360045363935096 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 7.221256780308788E-5 2 0.0 0.0 0.0 0.0 7.221256780308788E-5 3 0.0 0.0 0.0 0.0 7.221256780308788E-5 4 0.0 0.0 0.0 3.610628390154394E-5 7.221256780308788E-5 5 0.0 0.0 0.0 3.610628390154394E-5 7.221256780308788E-5 6 0.0 0.0 0.0 7.221256780308788E-5 7.221256780308788E-5 7 0.0 0.0 0.0 1.0831885170463182E-4 7.221256780308788E-5 8 0.0 0.0 0.0 1.0831885170463182E-4 7.221256780308788E-5 9 0.0 0.0 0.0 1.0831885170463182E-4 1.0831885170463182E-4 10-11 0.0 0.0 0.0 1.0831885170463182E-4 1.0831885170463182E-4 12-13 0.0 0.0 0.0 1.0831885170463182E-4 1.805314195077197E-4 14-15 0.0 0.0 0.0 1.0831885170463182E-4 2.1663770340926366E-4 16-17 0.0 0.0 0.0 1.263719936554038E-4 2.888502712123515E-4 18-19 0.0 0.0 0.0 1.4442513560617576E-4 3.2495655511389544E-4 20-21 0.0 0.0 0.0 1.8053141950771968E-4 3.2495655511389544E-4 22-23 0.0 0.0 0.0 2.888502712123515E-4 3.9716912291698335E-4 24-25 0.0 0.0 0.0 3.610628390154394E-4 5.054879746216152E-4 26-27 0.0 0.0 0.0 5.415942585231591E-4 5.415942585231591E-4 28-29 0.0 0.0 0.0 0.0015164639238648455 5.596474004739311E-4 30-31 0.0 0.0 0.0 0.004693816907200712 5.77700542424703E-4 32-33 0.0 0.0 0.0 0.012962155920654275 5.77700542424703E-4 34-35 0.0 0.0 0.0 0.024696698188656056 6.499131102277909E-4 36-37 0.0 0.0 0.0 0.043273381256000415 6.499131102277909E-4 38-39 0.0 0.0 0.0 0.06883663025829352 7.040725360801069E-4 40-41 0.0 0.0 0.0 0.09477899524155284 7.221256780308788E-4 42-43 0.0 0.0 0.0 0.12066720079895984 7.221256780308788E-4 44-45 0.0 0.0 0.0 0.15480569222786966 7.401788199816508E-4 46-47 0.0 0.0 0.0 0.1954794210429589 7.762851038831947E-4 48-49 0.0 0.0 0.0 0.2369474881038821 8.304445297355106E-4 50-51 0.0 0.0 0.0 0.2859798216421788 8.304445297355106E-4 52-53 0.0 0.0 0.0 0.3512419297942194 8.665508136370545E-4 54-55 0.0 1.805314195077197E-5 0.0 0.4252778649343353 9.387633814401425E-4 56-57 0.0 3.610628390154394E-5 0.0 0.5118968400141393 9.387633814401425E-4 58-59 0.0 3.610628390154394E-5 0.0 0.6061884004230211 9.387633814401425E-4 60-61 0.0 3.610628390154394E-5 0.0 0.703187932124519 9.568165233909145E-4 62-63 0.0 3.610628390154394E-5 0.0 0.8027149036991249 9.748696653416864E-4 64-65 0.0 3.610628390154394E-5 0.0 0.909029856647221 9.748696653416864E-4 66-67 0.0 3.610628390154394E-5 0.0 1.0291735163296085 9.929228072924584E-4 68-69 0.0 3.610628390154394E-5 0.0 1.1612141965575546 0.0010109759492432304 70-71 0.0 3.610628390154394E-5 0.0 1.3008010901209235 0.0010109759492432304 72-73 0.0 3.610628390154394E-5 0.0 1.4436556023773823 0.0011012416589970902 74-75 0.0 3.610628390154394E-5 0.0 1.5949228787829006 0.0011192948009478621 76-77 0.0 3.610628390154394E-5 0.0 1.7627629394992275 0.0011192948009478621 78-79 0.0 3.610628390154394E-5 0.0 1.944973301208369 0.0011192948009478621 80-81 0.0 3.610628390154394E-5 0.0 2.13933342745038 0.0011192948009478621 82-83 0.0 3.610628390154394E-5 0.0 2.3412397670278136 0.001155401084849406 84-85 0.0 3.610628390154394E-5 0.0 2.5481287737836604 0.001155401084849406 86-87 0.0 7.221256780308788E-5 0.0 2.771428086572759 0.001155401084849406 88-89 0.0 7.221256780308788E-5 0.0 3.0048913182801424 0.001155401084849406 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2350 0.0 52.615864 1 GTATCAA 4505 0.0 46.302322 1 ATCAACG 5540 0.0 37.358383 3 TCAACGC 5645 0.0 37.357574 4 TATCAAC 5795 0.0 35.550446 2 CAACGCA 5820 0.0 35.51289 5 AACGCAG 6270 0.0 33.389248 6 ACGCAGA 7260 0.0 28.732367 7 GTACATG 5230 0.0 27.870567 1 CGCAGAG 7680 0.0 27.41441 8 TACATGG 5525 0.0 25.464808 2 GCAGAGT 8035 0.0 24.749502 9 ACATGGG 5765 0.0 24.224012 3 GTGGTAT 1120 0.0 23.785229 1 TGGTATC 1215 0.0 21.09155 2 AGAGTAC 7170 0.0 20.136318 10-11 GAGTACT 5245 0.0 19.865875 12-13 CATGGGG 4385 0.0 19.809332 4 TATACCG 275 2.3504981E-8 19.316357 5 CAGAGTA 7740 0.0 19.312443 10-11 >>END_MODULE