FastQCFastQC Report
Sat 18 Jun 2016
SRR1930906_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1930906_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2769601
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55120.1990178368653102No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45350.16374199749350177No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30370.10965478420898896No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA33600.056.387191
GTATCAA62050.040.5584071
TCAACGC73800.033.770084
ATCAACG74250.033.5020833
CAACGCA74400.033.4188465
TATCAAC78900.031.7104172
AACGCAG80650.031.2403136
ACGCAGA93150.026.6406047
CGCAGAG96000.025.6520088
GTACATG72050.025.175391
TACATGG74700.023.7059062
GTGGTAT15650.023.3627091
ACATGGG73500.023.1223873
GCAGAGT110750.022.0213859
CATGGGG51600.020.2396094
GAGTACT73400.019.68414112-13
CAGAGTA106700.019.49981110-11
TGGTATC17000.018.9901492
AGAGTAC102900.018.95185310-11
GTACTTT79550.017.92377914-15
Produced by FastQC (version 0.11.5)