FastQCFastQC Report
Sat 18 Jun 2016
SRR1930905_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1930905_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1422163
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN26110.18359358245151927No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA11850.057.9550321
GTATCAA23150.045.1533431
ATCAACG29000.035.681333
TCAACGC29400.035.1891984
CAACGCA29450.034.3679055
TATCAAC30350.033.93272
AACGCAG31600.032.823176
ACGCAGA36800.027.5873267
GTACATG25350.027.0914041
CGCAGAG37650.026.5860488
TACATGG25550.024.895832
GCAGAGT40600.022.8449619
ACATGGG27200.022.8255563
GTGGTAT6800.021.5357321
TGGTATC6900.020.485942
GAGTACA19000.020.464811
AGAGTAC36350.020.2605410-11
CAGAGTA38750.019.74765610-11
GAGTACT28300.019.29636412-13
ATACCGA1200.006271065718.8721286
Produced by FastQC (version 0.11.5)