##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1930905_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1422163 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 22.221964008345036 30.0 10.0 31.0 2.0 33.0 2 21.19889632904245 28.0 10.0 31.0 2.0 33.0 3 21.617683064458856 28.0 10.0 31.0 2.0 33.0 4 23.519417957013367 32.0 2.0 35.0 2.0 37.0 5 23.497443682615845 32.0 2.0 35.0 2.0 37.0 6 24.1986755385986 32.0 10.0 35.0 2.0 37.0 7 24.25172993531684 32.0 10.0 35.0 2.0 37.0 8 24.958094817542012 32.0 16.0 35.0 2.0 37.0 9 26.03426189543674 32.0 17.0 37.0 2.0 39.0 10-11 26.239131168508813 32.5 17.0 37.0 2.0 39.0 12-13 26.12960574842687 33.0 13.5 37.0 2.0 39.0 14-15 26.307545970468926 33.0 13.5 38.0 2.0 40.0 16-17 25.45587988156069 32.0 10.0 38.0 2.0 40.0 18-19 24.874614583560394 32.0 10.0 38.0 2.0 40.0 20-21 24.36386440935392 32.0 2.0 38.0 2.0 40.0 22-23 24.28072555677514 31.0 8.0 37.0 2.0 40.0 24-25 23.969500683114383 31.0 2.0 37.0 2.0 39.0 26-27 23.617417272141097 30.5 2.0 37.0 2.0 39.0 28-29 23.494542116480318 30.0 2.0 37.0 2.0 39.0 30-31 23.357373943774377 30.0 2.0 37.0 2.0 39.0 32-33 23.016893633148943 30.0 2.0 37.0 2.0 39.0 34-35 20.20575806008172 24.0 2.0 35.0 2.0 39.0 36-37 18.639461158812317 17.0 2.0 34.0 2.0 38.0 38-39 17.738473719257215 15.5 2.0 33.0 2.0 38.0 40-41 18.913646326054046 18.5 2.0 34.0 2.0 38.0 42-43 18.786541697400367 18.5 2.0 34.0 2.0 38.0 44-45 16.933704505039156 12.0 2.0 32.5 2.0 38.0 46-47 17.665794286590213 17.0 2.0 32.5 2.0 38.0 48-49 17.052313623684487 13.0 2.0 33.0 2.0 38.0 50-51 15.278498315593923 9.5 2.0 30.5 2.0 37.0 52-53 17.976867982080815 18.5 2.0 32.0 2.0 37.5 54-55 19.44863317355324 23.5 2.0 34.5 2.0 38.0 56-57 19.61359281601335 24.0 2.0 35.0 2.0 39.0 58-59 19.24879883670156 23.0 2.0 35.0 2.0 38.5 60-61 18.911566044117308 22.5 2.0 34.5 2.0 38.0 62-63 18.418360623922858 21.0 2.0 34.0 2.0 38.0 64-65 17.913908251023265 19.0 2.0 34.0 2.0 38.0 66-67 17.491760789726634 17.5 2.0 33.5 2.0 37.0 68-69 17.01706766383319 12.5 2.0 33.0 2.0 36.5 70-71 16.43637719445661 8.0 2.0 32.0 2.0 36.0 72-73 15.946107794957399 7.0 2.0 31.5 2.0 35.5 74-75 15.4188704107757 2.0 2.0 31.0 2.0 35.0 76-77 14.856937284966632 2.0 2.0 31.0 2.0 35.0 78-79 14.347049881061455 2.0 2.0 30.0 2.0 34.0 80-81 13.838419365431388 2.0 2.0 30.0 2.0 34.0 82-83 13.349375563841837 2.0 2.0 29.0 2.0 34.0 84-85 12.829938270085776 2.0 2.0 29.0 2.0 34.0 86-87 12.293411514713855 2.0 2.0 28.5 2.0 34.0 88-89 11.757245125910321 2.0 2.0 27.0 2.0 33.5 90-91 11.203100136904137 2.0 2.0 26.0 2.0 33.0 92-93 10.601916236043266 2.0 2.0 25.0 2.0 32.5 94-95 9.97357159481719 2.0 2.0 23.5 2.0 32.0 96-97 9.298666538223818 2.0 2.0 19.0 2.0 32.0 98-99 8.551986656944386 2.0 2.0 2.0 2.0 32.0 100-101 7.625859694001321 2.0 2.0 2.0 2.0 30.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 6.905780629937734 1101 2 8.02258109923157 1101 3 7.347699077472178 1101 4 9.18193993715569 1101 5 8.933260040417146 1101 6 8.554951329146942 1101 7 8.090621876971628 1101 8 7.615920097702318 1101 9 7.8615748765363165 1101 10-11 7.433900980639397 1101 12-13 7.16205561320626 1101 14-15 7.75600430402049 1101 16-17 8.510593896147629 1101 18-19 8.668798823169954 1101 20-21 9.409031403360625 1101 22-23 9.065446699327868 1101 24-25 8.964449215477767 1101 26-27 9.08115093583498 1101 28-29 9.054522237003418 1101 30-31 8.977738798309549 1101 32-33 9.157000626722184 1101 34-35 11.349091963256544 1101 36-37 5.7005771215802135 1101 38-39 8.54528916036534 1101 40-41 10.270164388095811 1101 42-43 10.686410466228672 1101 44-45 5.357054607899499 1101 46-47 6.831376658539622 1101 48-49 9.731668248315717 1101 50-51 -2.671735905498167 1101 52-53 6.336247030469927 1101 54-55 8.847560522731495 1101 56-57 9.40708421432413 1101 58-59 9.58754492283872 1101 60-61 9.29664289431727 1101 62-63 9.499550741622308 1101 64-65 9.496652121277048 1101 66-67 9.541878200536932 1101 68-69 9.333557105224575 1101 70-71 9.219775670856606 1101 72-73 9.01900193503637 1101 74-75 9.078176563840834 1101 76-77 8.818625560759658 1101 78-79 8.85863273135631 1101 80-81 8.271186407836234 1101 82-83 8.284165625617545 1101 84-85 8.525316943916152 1101 86-87 7.9066100356288995 1101 88-89 7.832610723259476 1101 90-91 7.707629189322178 1101 92-93 7.341439837393864 1101 94-95 7.087117986491901 1101 96-97 6.777341293510956 1101 98-99 6.512813112578131 1101 100-101 6.090764321114545 1102 1 6.5479471513528615 1102 2 7.440616603759629 1102 3 7.269474809097861 1102 4 9.087602221015082 1102 5 8.968901925908412 1102 6 8.428405663326519 1102 7 8.132860076863007 1102 8 7.250325134773039 1102 9 7.442847848382776 1102 10-11 7.121135738700854 1102 12-13 6.6342809930706 1102 14-15 7.002675359809711 1102 16-17 7.833758832426415 1102 18-19 7.965201163614299 1102 20-21 8.211745081003254 1102 22-23 8.028121790583729 1102 24-25 8.269820048968985 1102 26-27 8.395967855744544 1102 28-29 8.168955977041058 1102 30-31 7.932609777052303 1102 32-33 8.234239710753354 1102 34-35 10.135862705807664 1102 36-37 1.3575000940582065 1102 38-39 3.844433123585123 1102 40-41 8.176687129434399 1102 42-43 8.96585153199604 1102 44-45 1.4900473895296038 1102 46-47 2.2621927195705283 1102 48-49 7.610676874994002 1102 50-51 -3.872094613643622 1102 52-53 5.112562030533081 1102 54-55 7.777165628007285 1102 56-57 8.326661437619947 1102 58-59 8.671784499462067 1102 60-61 8.471329971982634 1102 62-63 8.364110787344963 1102 64-65 8.322689722479481 1102 66-67 8.44572326135312 1102 68-69 8.383499951902099 1102 70-71 8.245793063149431 1102 72-73 8.159545428399008 1102 74-75 7.942346640760995 1102 76-77 7.8156976370988165 1102 78-79 7.909325518038637 1102 80-81 7.522469199949706 1102 82-83 7.305411031248253 1102 84-85 7.171357892219234 1102 86-87 6.914007601718348 1102 88-89 6.680609725444567 1102 90-91 6.370530367754222 1102 92-93 6.224765750268205 1102 94-95 6.126531675502044 1102 96-97 5.9168167144246535 1102 98-99 5.732700258448565 1102 100-101 5.066828604392935 1103 1 6.466278295812316 1103 2 7.6117476395015515 1103 3 7.207343987056419 1103 4 8.788235334631125 1103 5 9.051282943496169 1103 6 8.22414171625785 1103 7 7.826808826092748 1103 8 7.055839540882797 1103 9 7.384724429092053 1103 10-11 6.91701754366078 1103 12-13 6.59976732765087 1103 14-15 7.137146129525657 1103 16-17 7.657705942594195 1103 18-19 7.890050950847456 1103 20-21 8.289533872309175 1103 22-23 7.988978812511885 1103 24-25 8.068528457771784 1103 26-27 7.983789677261068 1103 28-29 8.113118686099263 1103 30-31 7.921536513057514 1103 32-33 7.782933855567592 1103 34-35 10.056841765235074 1103 36-37 -2.9465918033738614 1103 38-39 -1.4811888381853109 1103 40-41 6.3424232653562065 1103 42-43 8.55931333459074 1103 44-45 0.8599206526944876 1103 46-47 0.7073225270533641 1103 48-49 6.796659316283428 1103 50-51 -4.578983912643793 1103 52-53 3.972055000202136 1103 54-55 6.977498419827743 1103 56-57 7.640251336018856 1103 58-59 7.873711227168233 1103 60-61 7.898718749180173 1103 62-63 7.650589511698314 1103 64-65 7.590408967764066 1103 66-67 7.535267653997671 1103 68-69 7.428346191292288 1103 70-71 7.439971332922436 1103 72-73 7.271911165364836 1103 74-75 7.291935474807008 1103 76-77 6.9681096516304475 1103 78-79 7.12088899641858 1103 80-81 6.7885164464293535 1103 82-83 6.331757136816291 1103 84-85 5.920936456193484 1103 86-87 5.775057420902842 1103 88-89 5.877928147463601 1103 90-91 5.736169560422839 1103 92-93 5.579407926444542 1103 94-95 5.318194296382863 1103 96-97 4.950067627838612 1103 98-99 4.553466499166602 1103 100-101 4.313320441241952 1104 1 6.8458592193502845 1104 2 7.63357255655438 1104 3 6.960288264791693 1104 4 9.022870783299012 1104 5 9.025700133083475 1104 6 8.015539985565614 1104 7 7.871473449966473 1104 8 7.25066232925553 1104 9 7.303848459393045 1104 10-11 6.4684834391929265 1104 12-13 6.4684095310131475 1104 14-15 6.7179760174627425 1104 16-17 7.575500043335868 1104 18-19 7.881330596780966 1104 20-21 7.904933009737871 1104 22-23 7.781202589511832 1104 24-25 7.852025934671442 1104 26-27 8.114135161644803 1104 28-29 7.846494071104949 1104 30-31 7.499342842808694 1104 32-33 7.7578396109319065 1104 34-35 9.666996996357408 1104 36-37 -2.7998788699575474 1104 38-39 -1.496960692832534 1104 40-41 6.058933220998792 1104 42-43 7.941694840047216 1104 44-45 0.5382139626136606 1104 46-47 0.3480859581527085 1104 48-49 6.642082753170243 1104 50-51 -4.726597670180391 1104 52-53 4.097805686969036 1104 54-55 6.499638476671823 1104 56-57 7.241324000515881 1104 58-59 7.462479565727467 1104 60-61 7.25845825343951 1104 62-63 7.156675772792617 1104 64-65 7.2304405412035315 1104 66-67 7.054598705375366 1104 68-69 7.299230544898954 1104 70-71 7.09877552606547 1104 72-73 6.847209259859204 1104 74-75 6.72370523248631 1104 76-77 6.904341412171776 1104 78-79 6.456947925577532 1104 80-81 6.265397276019987 1104 82-83 6.280689130045742 1104 84-85 5.924811783720845 1104 86-87 5.730778793723124 1104 88-89 5.316739000164487 1104 90-91 5.1717903659915 1104 92-93 5.028536634292118 1104 94-95 4.752183906679807 1104 96-97 4.324039954997319 1104 98-99 3.997122378406617 1104 100-101 3.911743652179485 1105 1 5.972392161034595 1105 2 6.92843810901266 1105 3 6.743270914939686 1105 4 8.631985518547332 1105 5 8.513089677477804 1105 6 7.599589245699153 1105 7 7.339756527189184 1105 8 6.404634423794384 1105 9 6.615090515531691 1105 10-11 6.435488616338578 1105 12-13 6.203305777418695 1105 14-15 6.473312344657597 1105 16-17 6.946361206128959 1105 18-19 7.548032767174515 1105 20-21 7.768132156150248 1105 22-23 7.464429676967107 1105 24-25 7.5042776980752315 1105 26-27 7.781103961125684 1105 28-29 7.61937992951016 1105 30-31 7.424492169143033 1105 32-33 6.99326092234438 1105 34-35 8.891809091907536 1105 36-37 -3.4298625398037874 1105 38-39 -2.0554561650539505 1105 40-41 5.519205486985538 1105 42-43 7.854211940358741 1105 44-45 -2.8807675470453837 1105 46-47 -3.9462578065657326 1105 48-49 4.682160686581792 1105 50-51 -9.04466931685729 1105 52-53 0.6043255757666834 1105 54-55 5.033834652007489 1105 56-57 6.142847742443269 1105 58-59 6.413824087962787 1105 60-61 6.496687480482024 1105 62-63 6.476025319546579 1105 64-65 6.05751273377949 1105 66-67 5.863688299303249 1105 68-69 6.082120495206222 1105 70-71 6.512263399709031 1105 72-73 6.373234037871173 1105 74-75 6.195005502252215 1105 76-77 6.117378989316302 1105 78-79 5.93518757432941 1105 80-81 5.687284551140943 1105 82-83 5.3925644946417055 1105 84-85 5.315194690244759 1105 86-87 5.153305135927864 1105 88-89 5.176023216939788 1105 90-91 4.924256743571997 1105 92-93 4.7220651085931955 1105 94-95 4.300651877948475 1105 96-97 4.134528473794317 1105 98-99 3.7644338395714723 1105 100-101 3.2527009231710835 1106 1 6.041009289115653 1106 2 7.12787437657142 1106 3 6.834720943233158 1106 4 8.118977866620728 1106 5 8.161655792714832 1106 6 7.157359354879464 1106 7 7.498379403008215 1106 8 6.63975653094807 1106 9 6.555694700294531 1106 10-11 6.054914456939688 1106 12-13 6.122309419730417 1106 14-15 6.285304548035807 1106 16-17 6.628459651390326 1106 18-19 6.890766464368003 1106 20-21 7.634624134917008 1106 22-23 7.327182286934335 1106 24-25 7.338153601251449 1106 26-27 7.493393390674935 1106 28-29 7.32186792982715 1106 30-31 6.8778397701636464 1106 32-33 6.981299082694246 1106 34-35 9.09783077125321 1106 36-37 0.1087345669158104 1106 38-39 1.4964014762542561 1106 40-41 6.851411830333408 1106 42-43 7.903394056390038 1106 44-45 -2.942766539966895 1106 46-47 -3.823892427626852 1106 48-49 4.4487357426456136 1106 50-51 -8.987380040907544 1106 52-53 0.5316765720041623 1106 54-55 4.937945152826462 1106 56-57 5.951717646334501 1106 58-59 6.439997888695704 1106 60-61 6.137656208068183 1106 62-63 6.225428173647977 1106 64-65 5.90921441593893 1106 66-67 5.940643876990983 1106 68-69 5.637900711679123 1106 70-71 5.658009294484021 1106 72-73 5.817877666986698 1106 74-75 5.7431300077842415 1106 76-77 5.6854146653611455 1106 78-79 5.7212842369460954 1106 80-81 5.644574539436215 1106 82-83 5.435512251665989 1106 84-85 4.82204033075692 1106 86-87 4.582286958205669 1106 88-89 4.691129171658654 1106 90-91 4.43854350093509 1106 92-93 4.384956937589308 1106 94-95 4.028506502473375 1106 96-97 3.6239341149475877 1106 98-99 3.1981705682403945 1106 100-101 3.0172578153144016 1107 1 5.843996925815947 1107 2 7.035403873324981 1107 3 6.560675105567441 1107 4 7.614872693532778 1107 5 8.005416806326558 1107 6 6.519150253625249 1107 7 6.911888907371576 1107 8 6.235873690352399 1107 9 6.374185995486506 1107 10-11 5.813866057540761 1107 12-13 5.65251937314229 1107 14-15 5.689686672363155 1107 16-17 6.740076085759281 1107 18-19 6.88830774404229 1107 20-21 7.419186930695499 1107 22-23 6.963132995049296 1107 24-25 6.955779193685128 1107 26-27 7.199681735577858 1107 28-29 6.987804812297778 1107 30-31 6.8632921589228815 1107 32-33 6.567343116100229 1107 34-35 8.788107845231362 1107 36-37 -0.20001061339618786 1107 38-39 0.9089224952664878 1107 40-41 6.271324571843831 1107 42-43 7.128604115006556 1107 44-45 -3.2710526071891586 1107 46-47 -3.9315323454269553 1107 48-49 4.189233351869955 1107 50-51 -8.997951354755807 1107 52-53 0.360180719697464 1107 54-55 4.576175514186897 1107 56-57 5.836335543048083 1107 58-59 6.235623172529078 1107 60-61 6.089152262017127 1107 62-63 6.105971143610297 1107 64-65 6.243122575786874 1107 66-67 6.104822088406738 1107 68-69 5.792415656876269 1107 70-71 5.715295636991783 1107 72-73 5.426465522512659 1107 74-75 5.365189757011429 1107 76-77 5.372043987299646 1107 78-79 5.398627714345507 1107 80-81 5.220740952150548 1107 82-83 5.001091569834658 1107 84-85 4.900077851995107 1107 86-87 4.414593158090996 1107 88-89 4.379047469727974 1107 90-91 4.219229067032542 1107 92-93 3.8956343327923264 1107 94-95 3.734537753514463 1107 96-97 3.3164925955820426 1107 98-99 3.345284147179827 1107 100-101 3.2531831866429783 1108 1 6.138951820747984 1108 2 6.423627295356383 1108 3 6.596943443067815 1108 4 8.22813992710633 1108 5 8.358352223949343 1108 6 7.655951240248772 1108 7 7.647792576909399 1108 8 6.4785502078973956 1108 9 5.4591981067864275 1108 10-11 5.984117090146473 1108 12-13 5.511489177288308 1108 14-15 6.2762820238212065 1108 16-17 6.150556568581081 1108 18-19 6.698349491258991 1108 20-21 7.5875650087996505 1108 22-23 7.586599148085806 1108 24-25 7.104055570537042 1108 26-27 7.50423662980111 1108 28-29 7.420289631088984 1108 30-31 7.319154134402591 1108 32-33 7.257143443557176 1108 34-35 9.295117248986841 1108 36-37 -3.289978656220754 1108 38-39 -1.9864015861086894 1108 40-41 5.204989954435103 1108 42-43 7.478476933067721 1108 44-45 -2.885119213136896 1108 46-47 -3.6876958630375096 1108 48-49 4.826598927072535 1108 50-51 -9.024684812243994 1108 52-53 0.8963994425090576 1108 54-55 5.399982901105275 1108 56-57 6.475977635086874 1108 58-59 6.705483799552429 1108 60-61 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-13.748321902610552 2313 74-75 -13.222928612466271 2313 76-77 -12.700495364490761 2313 78-79 -12.212729668041526 2313 80-81 -11.696983501259941 2313 82-83 -11.21083828266802 2313 84-85 -10.681312666413302 2313 86-87 -10.162745386755446 2313 88-89 -9.635973898360579 2313 90-91 -9.072972686654893 2313 92-93 -8.46378930394132 2313 94-95 -7.90118610707535 2313 96-97 -7.212678826922138 2313 98-99 -6.43770654465075 2313 100-101 -5.5077353314822926 2314 1 -20.09568135836636 2314 2 -19.089261006031588 2314 3 -19.49294419738162 2314 4 -21.39115947804899 2314 5 -21.37595048589864 2314 6 -22.053235805355985 2314 7 -22.068003854022525 2314 8 -22.803670545572533 2314 9 -23.881846176095266 2314 10-11 -24.062809545676398 2314 12-13 -23.960751404337604 2314 14-15 -24.142753005921026 2314 16-17 -23.304830080460558 2314 18-19 -22.72630351601133 2314 20-21 -22.259097251610132 2314 22-23 -22.141424645701374 2314 24-25 -21.837451369317552 2314 26-27 -21.500720409194262 2314 28-29 -21.32888419574512 2314 30-31 -21.197699798181677 2314 32-33 -20.85323329140647 2314 34-35 -18.183071779433515 2314 36-37 -16.56294627023265 2314 38-39 -15.692283939049865 2314 40-41 -16.865069530032844 2314 42-43 -16.759130469443843 2314 44-45 -14.894041883328569 2314 46-47 -15.577980066606578 2314 48-49 -15.032951634725219 2314 50-51 -13.209747601404601 2314 52-53 -15.863679870114868 2314 54-55 -17.284749418788973 2314 56-57 -17.436117540061836 2314 58-59 -17.10251355669344 2314 60-61 -16.74429277819735 2314 62-63 -16.26835861510284 2314 64-65 -15.771622732524119 2314 66-67 -15.34189372928763 2314 68-69 -14.863080321676012 2314 70-71 -14.273645381774973 2314 72-73 -13.804097480168307 2314 74-75 -13.286955015106535 2314 76-77 -12.732178532807593 2314 78-79 -12.240782473322055 2314 80-81 -11.751108913801195 2314 82-83 -11.262983497189472 2314 84-85 -10.743688904037064 2314 86-87 -10.223141426359406 2314 88-89 -9.69736003697444 2314 90-91 -9.136669056291856 2314 92-93 -8.518574782489175 2314 94-95 -7.90118610707535 2314 96-97 -7.2354511041498615 2314 98-99 -6.490841858182103 2314 100-101 -5.563840942043349 2315 1 -20.09568135836636 2315 2 -19.089261006031588 2315 3 -19.49294419738162 2315 4 -17.301075226590793 2315 5 -21.37595048589864 2315 6 -22.053235805355985 2315 7 -22.068003854022525 2315 8 -22.803670545572533 2315 9 -20.13071202185029 2315 10-11 -18.736821211262917 2315 12-13 -16.07351874069535 2315 14-15 -16.044567652712992 2315 16-17 -20.026476224336125 2315 18-19 -22.72630351601133 2315 20-21 -22.259097251610132 2315 22-23 -22.141424645701374 2315 24-25 -21.837451369317552 2315 26-27 -21.500720409194262 2315 28-29 -21.32888419574512 2315 30-31 -21.197699798181677 2315 32-33 -20.85323329140647 2315 34-35 -18.183071779433515 2315 36-37 -16.56294627023265 2315 38-39 -15.692283939049865 2315 40-41 -16.865069530032844 2315 42-43 -16.759130469443843 2315 44-45 -14.894041883328569 2315 46-47 -15.577980066606578 2315 48-49 -15.032951634725219 2315 50-51 -13.209747601404601 2315 52-53 -15.863679870114868 2315 54-55 -17.284749418788973 2315 56-57 -17.436117540061836 2315 58-59 -17.10251355669344 2315 60-61 -16.74429277819735 2315 62-63 -16.26835861510284 2315 64-65 -15.771622732524119 2315 66-67 -15.34189372928763 2315 68-69 -14.863080321676012 2315 70-71 -14.273645381774973 2315 72-73 -13.804097480168307 2315 74-75 -13.286955015106535 2315 76-77 -12.732178532807593 2315 78-79 -12.240782473322055 2315 80-81 -11.751108913801195 2315 82-83 -11.262983497189472 2315 84-85 -10.743688904037064 2315 86-87 -10.223141426359406 2315 88-89 -9.69736003697444 2315 90-91 -9.136669056291856 2315 92-93 -8.518574782489175 2315 94-95 -7.90118610707535 2315 96-97 -7.2354511041498615 2315 98-99 -6.490841858182103 2315 100-101 -5.563840942043349 2316 1 -20.09568135836636 2316 2 -19.089261006031588 2316 3 -19.49294419738162 2316 4 -21.39115947804899 2316 5 -21.37595048589864 2316 6 -22.053235805355985 2316 7 -22.068003854022525 2316 8 -22.803670545572533 2316 9 -23.881846176095266 2316 10-11 -24.062809545676398 2316 12-13 -23.960751404337604 2316 14-15 -24.142753005921026 2316 16-17 -23.304830080460558 2316 18-19 -22.72630351601133 2316 20-21 -22.259097251610132 2316 22-23 -22.141424645701374 2316 24-25 -21.837451369317552 2316 26-27 -21.500720409194262 2316 28-29 -21.32888419574512 2316 30-31 -21.197699798181677 2316 32-33 -20.85323329140647 2316 34-35 -18.183071779433515 2316 36-37 -16.56294627023265 2316 38-39 -15.692283939049865 2316 40-41 -16.865069530032844 2316 42-43 -16.759130469443843 2316 44-45 -14.894041883328569 2316 46-47 -15.577980066606578 2316 48-49 -15.032951634725219 2316 50-51 -13.209747601404601 2316 52-53 -15.863679870114868 2316 54-55 -17.284749418788973 2316 56-57 -17.436117540061836 2316 58-59 -17.10251355669344 2316 60-61 -16.74429277819735 2316 62-63 -16.26835861510284 2316 64-65 -15.771622732524119 2316 66-67 -15.34189372928763 2316 68-69 -14.863080321676012 2316 70-71 -14.273645381774973 2316 72-73 -13.804097480168307 2316 74-75 -13.286955015106535 2316 76-77 -12.732178532807593 2316 78-79 -12.240782473322055 2316 80-81 -11.751108913801195 2316 82-83 -11.262983497189472 2316 84-85 -10.743688904037064 2316 86-87 -10.223141426359406 2316 88-89 -9.69736003697444 2316 90-91 -9.136669056291856 2316 92-93 -8.518574782489175 2316 94-95 -7.90118610707535 2316 96-97 -7.2354511041498615 2316 98-99 -6.490841858182103 2316 100-101 -5.563840942043349 >>END_MODULE >>Per sequence quality scores fail #Quality Count 2 256994.0 3 16454.0 4 31480.0 5 21042.0 6 18892.0 7 23419.0 8 29490.0 9 33863.0 10 34551.0 11 31949.0 12 28326.0 13 26132.0 14 24780.0 15 26211.0 16 28112.0 17 29601.0 18 30197.0 19 31379.0 20 32647.0 21 34442.0 22 35685.0 23 37590.0 24 39654.0 25 41149.0 26 42800.0 27 45182.0 28 47386.0 29 49151.0 30 50172.0 31 49108.0 32 47424.0 33 45063.0 34 40742.0 35 32376.0 36 20635.0 37 7375.0 38 710.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.12762379567531 20.332561774190356 14.078234118740973 23.461580311393355 2 15.327521005192981 20.80716392365409 40.66903798148792 23.196277089665003 3 18.76646691284515 25.62666905178692 30.43933063303647 25.167533402331454 4 12.513505174063146 16.871478073604678 38.8286383416784 31.78637841065377 5 13.030931996519776 37.68743255246475 35.7981287883127 13.483506662702775 6 30.69480898159703 37.68362784756227 17.87820162731068 13.743361543530014 7 26.518351770397793 32.719824357826965 23.033543375139864 17.728280496635378 8 24.93731393517832 36.32001346277902 20.926207142485083 17.81646545955757 9 25.597792920889535 16.392220945917998 21.321722270557306 36.688263862635154 10-11 24.53686642969987 26.606103745683914 29.032221637103532 19.82480818751268 12-13 24.950418266651052 24.274093147634833 29.254152846339853 21.521335739374262 14-15 22.080100705853717 25.78054642813679 26.996052391383717 25.143300474625775 16-17 21.744372158208648 28.816819423320407 27.60664431721359 21.832164101257355 18-19 21.669190952859214 27.696517640900787 29.724078800660646 20.910212605579353 20-21 22.14819946321977 26.955535183164848 29.928028307482986 20.968237046132405 22-23 21.92373873166594 26.818432720215096 29.65916982182718 21.59865872629178 24-25 21.790637516688918 27.074098798397866 29.587116154873165 21.548147530040055 26-27 21.70199009247991 27.17798534127793 29.62650723456386 21.493517331678298 28-29 21.734174430940595 27.437589091169812 29.482164347815164 21.346072130074425 30-31 21.9421547627085 27.233580271161056 29.410479411277308 21.41378555485314 32-33 21.79288740515006 27.3277950361112 29.274371595922684 21.604945962816053 34-35 22.158314465167166 27.306638771178115 29.31741916668049 21.217627596974225 36-37 22.646552839128784 27.19742916838771 28.87322605155107 21.282791940932437 38-39 22.420532954234446 27.311348715200435 28.858627808113734 21.409490522451385 40-41 22.363757385934036 27.206000512680216 29.03338237543271 21.396859725953036 42-43 22.46227192456021 27.217942436863 28.663177208944703 21.65660842963209 44-45 22.670109267905495 27.445107069471884 28.360760423222644 21.52402323939998 46-47 22.03768403929458 27.4058798026786 28.741625578244705 21.81481057978211 48-49 22.55703521620313 27.48412085815023 28.127153613530304 21.83169031211634 50-51 22.758344231102196 27.68781929940805 28.003634627748177 21.55020184174158 52-53 21.89042840900748 27.802556048739934 28.53425029911842 21.772765243134167 54-55 21.621909086092845 27.76723820323688 28.60810347916501 22.00274923150527 56-57 21.734721326992492 28.01768594183953 28.34020990395051 21.907382827217468 58-59 21.793807075245024 27.82454180096036 28.56208732789689 21.81956379589772 60-61 21.691694685115735 27.956242725979568 28.525556058450796 21.826506530453898 62-63 21.759991597928565 27.84656524935571 28.506652986370874 21.88679016634486 64-65 21.549654532877973 27.69447379411706 28.495321028150734 22.260550644854234 66-67 21.55610214075937 27.886499929214853 28.372691612180823 22.184706317844956 68-69 21.728326196287437 27.776830502061543 28.397241966154652 22.09760133549637 70-71 21.612250286719124 27.732240491730785 28.186956447055735 22.468552774494363 72-73 21.741214703580255 27.869887287777388 28.128452124069884 22.26044588457247 74-75 21.781665543110247 27.964135602877626 27.975999406413038 22.278199447599086 76-77 21.780815987220137 27.750444749218634 28.04765916342265 22.421080100138578 78-79 21.65723468844941 27.834931003093978 28.017983611863023 22.489850696593592 80-81 21.75831557583363 27.953841403920826 27.818815264296216 22.469027755949327 82-83 21.872937271077536 27.800928495201628 27.940865594103187 22.38526863961765 84-85 21.85081554762787 27.95431804986292 27.780372165837814 22.4144942366714 86-87 21.735726683145373 28.05721687734638 27.652521897596827 22.554534541911416 88-89 21.78193894788222 27.899991545795466 27.564535090531773 22.75353441579054 90-91 21.906518269188666 27.807515228360497 27.59174702107731 22.694219481373523 92-93 21.885239012043378 27.702306225953688 27.69707273286665 22.715382029136283 94-95 21.72327986832379 27.575503015991863 27.673799657470344 23.027417458214003 96-97 21.83248155736167 27.489711739104163 27.575936035238602 23.101870668295568 98-99 21.673726249069006 27.53461876480768 27.451504552553374 23.34015043356994 100-101 21.986849179238156 27.76253681842978 27.332715449490756 22.917898552841308 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 17580.0 1 18875.5 2 14185.0 3 8707.5 4 11091.0 5 13827.5 6 14652.5 7 14100.5 8 12993.5 9 11863.0 10 10630.5 11 9333.5 12 8062.5 13 6780.5 14 5793.5 15 5035.0 16 4473.0 17 4320.5 18 4391.0 19 4516.5 20 4833.0 21 5372.0 22 6173.5 23 7129.0 24 8418.0 25 10122.5 26 11981.0 27 14085.0 28 16408.5 29 19173.0 30 22089.5 31 25091.5 32 28097.0 33 30264.0 34 31904.5 35 33908.5 36 36435.0 37 38595.5 38 40201.0 39 41357.0 40 42140.5 41 42883.0 42 43649.5 43 44279.5 44 44635.5 45 45039.5 46 44955.5 47 44414.0 48 43605.5 49 42445.0 50 40856.0 51 39018.5 52 37132.5 53 34986.0 54 32488.0 55 29502.5 56 26103.5 57 22557.5 58 19508.0 59 16570.5 60 13594.0 61 11064.5 62 8887.0 63 6984.0 64 5321.0 65 3950.5 66 2973.5 67 2237.5 68 1657.5 69 1224.5 70 908.5 71 694.0 72 525.5 73 388.5 74 269.5 75 204.5 76 163.0 77 113.0 78 64.5 79 41.5 80 25.5 81 14.5 82 7.5 83 8.0 84 4.5 85 1.0 86 1.0 87 0.5 88 0.5 89 0.5 90 0.0 91 0.0 92 0.5 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 14.698385487458188 2 21.77668804490062 3 17.453554901934588 4 22.747744105281882 5 22.658021619181486 6 16.441294000758 7 15.225399620156058 8 12.254432157214048 9 3.0975352333030743 10-11 6.598927127199906 12-13 3.6174826654891175 14-15 7.554900528279811 16-17 11.697182390485478 18-19 14.831598065763208 20-21 15.246353617693611 22-23 13.122335484751044 24-25 15.733991110723593 26-27 13.21571437310632 28-29 13.206889786895031 30-31 14.076832261843403 32-33 12.527291175484104 34-35 19.472873362617367 36-37 24.160486526509267 38-39 23.310021425110904 40-41 18.39419953971521 42-43 18.33545099963928 44-45 23.637410057778187 46-47 12.986521235610827 48-49 21.79310669733357 50-51 21.416637895937384 52-53 11.405900730085088 54-55 13.065837038370425 56-57 12.564628667740617 58-59 13.596015365327322 60-61 13.000127270924642 62-63 12.964266402655673 64-65 14.051307761487255 66-67 12.335611318815072 68-69 13.399378270985816 70-71 11.988956258881718 72-73 11.332315634705727 74-75 11.393033006765048 76-77 11.483036754577359 78-79 11.900499450484931 80-81 11.862353330806666 82-83 10.357778960639532 84-85 10.259302203755828 86-87 10.40622629051663 88-89 11.837531984730301 90-91 9.273866638352986 92-93 11.324651253056084 94-95 11.441023286360284 96-97 10.98878961131741 98-99 10.83057989836608 100-101 10.932220849508811 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1422163.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 97.9886580257318 #Duplication Level Percentage of deduplicated Percentage of total 1 99.15450717748425 97.16017095524472 2 0.6512308706974439 1.2762647816914279 3 0.0971812698044345 0.28567986640119325 4 0.03522063271268036 0.13804890137330952 5 0.018441021034801386 0.09035054519122399 6 0.012685308875516534 0.07458098360122618 7 0.005892712239229677 0.04041932751177448 8 0.004847553329959301 0.03800041964087035 9 0.003117922451950411 0.027496893320542802 >10 0.01433681731914736 0.24931810026495665 >50 0.0012389902677339886 0.08295680409716819 >100 0.001010919637067146 0.14186321985585287 >500 1.4440207290147356E-4 0.12027300538786892 >1k 1.4440207290147356E-4 0.2745761964178349 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 2611 0.18359358245151927 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 7.031542797836817E-5 0.0 0.0 0.0 5 0.0 7.031542797836817E-5 0.0 0.0 0.0 6 0.0 7.031542797836817E-5 0.0 0.0 7.031542797836817E-5 7 0.0 7.031542797836817E-5 0.0 0.0 1.4063085595673633E-4 8 0.0 7.031542797836817E-5 0.0 0.0 1.4063085595673633E-4 9 0.0 7.031542797836817E-5 0.0 0.0 1.4063085595673633E-4 10-11 0.0 7.031542797836817E-5 0.0 0.0 1.4063085595673633E-4 12-13 0.0 7.031542797836817E-5 0.0 0.0 4.922079958485771E-4 14-15 0.0 7.031542797836817E-5 0.0 0.0 5.625234238269453E-4 16-17 0.0 7.031542797836817E-5 0.0 7.031542797836817E-5 6.328388518053135E-4 18-19 0.0 7.031542797836817E-5 0.0 1.0547314196755224E-4 7.031542797836816E-4 20-21 0.0 1.4063085595673633E-4 0.0 1.4063085595673633E-4 7.031542797836816E-4 22-23 0.0 1.4063085595673633E-4 0.0 2.4610399792428857E-4 7.734697077620497E-4 24-25 0.0 1.757885699459204E-4 0.0 4.2189256787020896E-4 7.734697077620497E-4 26-27 0.0 2.1094628393510448E-4 0.0 5.976811378161295E-4 7.734697077620497E-4 28-29 0.0 2.1094628393510448E-4 0.0 0.001757885699459204 7.734697077620497E-4 30-31 0.0 2.1094628393510448E-4 0.0 0.005133026242420876 7.734697077620497E-4 32-33 0.0 2.4610399792428857E-4 0.0 0.012867723320041373 7.734697077620497E-4 34-35 0.0 2.8126171191347266E-4 0.0 0.023415037516796598 7.734697077620497E-4 36-37 0.0 2.8126171191347266E-4 0.0 0.041099367653356186 7.734697077620497E-4 38-39 0.0 2.8126171191347266E-4 0.0 0.06444408974217442 7.734697077620497E-4 40-41 0.0 2.8126171191347266E-4 0.0 0.09053111352214901 7.734697077620497E-4 42-43 0.0 2.8126171191347266E-4 0.0 0.1168290835860587 7.734697077620497E-4 44-45 0.0 2.8126171191347266E-4 0.0 0.1497718615939242 7.734697077620497E-4 46-47 0.0 2.8126171191347266E-4 0.0 0.19101186010323712 7.734697077620497E-4 48-49 0.0 2.8126171191347266E-4 0.0 0.23288469746435536 8.437851357404179E-4 50-51 0.0 2.8126171191347266E-4 0.0 0.28256254733107244 8.437851357404179E-4 52-53 0.0 2.8126171191347266E-4 0.0 0.3467956907893118 0.0010195737056863383 54-55 0.0 2.8126171191347266E-4 0.0 0.41837679647129056 0.0011250468476538906 56-57 0.0 2.8126171191347266E-4 0.0 0.5023334174774621 0.0011250468476538906 58-59 0.0 2.8126171191347266E-4 0.0 0.5951849401228974 0.0011250468476538906 60-61 0.0 2.8126171191347266E-4 0.0 0.6948922169962234 0.0011250468476538906 62-63 0.0 2.8126171191347266E-4 0.0 0.7904157259048366 0.0011250468476538906 64-65 0.0 2.8126171191347266E-4 0.0 0.8896659524963032 0.0012305199896214427 66-67 7.031542797836817E-5 2.8126171191347266E-4 0.0 1.0087099720636805 0.001265677703610627 68-69 7.031542797836817E-5 2.8126171191347266E-4 0.0 1.1359105812765484 0.001265677703610627 70-71 7.031542797836817E-5 2.8126171191347266E-4 0.0 1.274853866961804 0.001300835417599811 72-73 7.031542797836817E-5 2.8126171191347266E-4 0.0 1.4142190452149297 0.0014766239875457312 74-75 7.031542797836817E-5 2.8126171191347266E-4 0.0 1.5509473949188664 0.0015469394155240995 76-77 1.0547314196755224E-4 2.8126171191347266E-4 0.0 1.7025826153542174 0.0015820971295132835 78-79 1.4063085595673633E-4 2.8126171191347266E-4 0.0 1.8812189601332618 0.0016172548435024678 80-81 1.4063085595673633E-4 3.1641942590265675E-4 0.0 2.073566813368088 0.0016172548435024678 82-83 1.4063085595673633E-4 3.515771398918408E-4 0.0 2.2709422197033673 0.0016875702714808359 84-85 1.4063085595673633E-4 3.515771398918408E-4 0.0 2.4624814455164423 0.0016875702714808359 86-87 1.4063085595673633E-4 3.515771398918408E-4 0.0 2.6614741066952243 0.0017227279854700201 88-89 1.4063085595673633E-4 3.515771398918408E-4 0.0 2.8794519334281654 0.0017578856994592041 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1185 0.0 57.955032 1 GTATCAA 2315 0.0 45.153343 1 ATCAACG 2900 0.0 35.68133 3 TCAACGC 2940 0.0 35.189198 4 CAACGCA 2945 0.0 34.367905 5 TATCAAC 3035 0.0 33.9327 2 AACGCAG 3160 0.0 32.82317 6 ACGCAGA 3680 0.0 27.587326 7 GTACATG 2535 0.0 27.091404 1 CGCAGAG 3765 0.0 26.586048 8 TACATGG 2555 0.0 24.89583 2 GCAGAGT 4060 0.0 22.844961 9 ACATGGG 2720 0.0 22.825556 3 GTGGTAT 680 0.0 21.535732 1 TGGTATC 690 0.0 20.48594 2 GAGTACA 1900 0.0 20.46481 1 AGAGTAC 3635 0.0 20.26054 10-11 CAGAGTA 3875 0.0 19.747656 10-11 GAGTACT 2830 0.0 19.296364 12-13 ATACCGA 120 0.0062710657 18.872128 6 >>END_MODULE