FastQCFastQC Report
Sat 18 Jun 2016
SRR1930905_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1930905_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1422163
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39490.2776756250865759No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33020.23218154318457168No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24770.17417131510241793No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA17900.059.688111
GTATCAA34150.042.4099241
TAACGCA1054.6860805E-836.1664854
CAACGCA41400.034.2767835
ATCAACG41650.034.0770343
TCAACGC41650.034.0770344
TATCAAC43850.032.2602352
AACGCAG44250.032.175256
ATAACGC1201.5150363E-731.6456723
TATAACG1754.996764E-927.1258142
ACGCAGA52600.026.7969077
CGCAGAG53350.026.242288
GCAGAGT60700.022.6737469
GTACATG34150.021.2744851
GAGTACT42200.021.08641612-13
TATACCG1404.880046E-420.340075
CAGAGTA58000.020.33351110-11
TACATGG36200.020.1944962
AGAGTAC55400.020.04564710-11
GTACTTT44800.019.59781614-15
Produced by FastQC (version 0.11.5)