FastQCFastQC Report
Sat 18 Jun 2016
SRR1930904_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1930904_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1967937
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN35330.17952810481229836No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA15350.055.2532541
GTATCAA30000.047.9601551
ATCAACG38000.037.583343
TCAACGC38250.037.460614
TATCAAC38850.036.5037732
CAACGCA38800.035.556355
AACGCAG41350.033.7138256
ACGCAGA47850.028.2950047
CGCAGAG49050.027.7580748
GTACATG36100.026.4308741
GCAGAGT52700.024.288579
TACATGG36600.024.2691172
ACATGGG37700.023.693243
CAGAGTA50450.020.05395710-11
CATGGGG27900.019.5302374
GAGTACA27050.019.4098871
AGAGTAC49250.019.1072910-11
GAGTACT37150.018.76219412-13
AGTACAT28450.018.094242
GTATTAG6050.017.52351
Produced by FastQC (version 0.11.5)