##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1930904_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1967937 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 22.552999918188437 30.0 10.0 31.0 2.0 33.0 2 21.454758968401936 28.0 10.0 31.0 2.0 33.0 3 21.895686701352737 28.0 10.0 31.0 2.0 33.0 4 23.78479646452097 32.0 2.0 35.0 2.0 37.0 5 23.775238739858032 32.0 10.0 35.0 2.0 37.0 6 24.529776105637527 32.0 11.0 35.0 2.0 37.0 7 24.607870577157705 32.0 11.0 35.0 2.0 37.0 8 25.38783812693191 32.0 17.0 35.0 2.0 37.0 9 26.51567199559742 33.0 17.0 37.0 2.0 39.0 10-11 26.760889957351274 33.0 17.0 37.0 2.0 39.0 12-13 26.68978453070398 33.0 17.0 37.0 2.0 39.0 14-15 26.897265003910185 33.5 16.5 38.0 2.0 40.0 16-17 26.013178013320548 32.5 10.5 38.0 2.0 40.0 18-19 25.40116909230326 32.0 10.0 38.0 2.0 40.0 20-21 24.88820424637577 32.0 9.0 38.0 2.0 40.0 22-23 24.822843414194665 32.0 10.0 38.0 2.0 40.0 24-25 24.523454510992984 32.0 8.0 38.0 2.0 40.0 26-27 24.18507375998317 31.0 5.0 37.0 2.0 39.5 28-29 24.098667792718974 31.0 5.0 37.0 2.0 39.0 30-31 24.004928765504182 30.5 2.0 37.0 2.0 39.0 32-33 23.697559677977495 30.0 2.0 37.0 2.0 39.0 34-35 20.836773484110516 25.0 2.0 36.0 2.0 39.0 36-37 19.24248489662017 18.0 2.0 35.0 2.0 38.5 38-39 18.3292651136698 17.0 2.0 34.0 2.0 38.0 40-41 19.620963221891756 22.0 2.0 34.5 2.0 38.0 42-43 19.541694932307283 22.0 2.0 35.0 2.0 38.0 44-45 17.61197792409005 15.5 2.0 33.5 2.0 38.0 46-47 18.43638083942728 18.5 2.0 33.0 2.0 38.0 48-49 17.854090857583348 15.0 2.0 33.0 2.0 38.0 50-51 15.941577143983777 10.0 2.0 31.0 2.0 37.5 52-53 18.815979373323437 21.0 2.0 33.5 2.0 37.5 54-55 20.416129174866878 26.0 2.0 35.5 2.0 38.5 56-57 20.63181773603525 27.0 2.0 36.0 2.0 39.0 58-59 20.273626645568427 26.0 2.0 36.0 2.0 39.0 60-61 19.957633298220422 26.0 2.0 35.0 2.0 39.0 62-63 19.486284621916248 24.5 2.0 34.5 2.0 38.0 64-65 18.98804255420778 22.5 2.0 34.0 2.0 38.0 66-67 18.577683889270844 22.0 2.0 34.0 2.0 38.0 68-69 18.115576616527868 21.0 2.0 34.0 2.0 37.0 70-71 17.532538643259414 18.5 2.0 33.0 2.0 36.5 72-73 17.06653160136732 16.0 2.0 32.5 2.0 36.0 74-75 16.531961643081054 10.0 2.0 32.0 2.0 35.5 76-77 15.966167362064944 7.5 2.0 31.5 2.0 35.0 78-79 15.468017522918672 4.5 2.0 31.0 2.0 35.0 80-81 14.964213793429362 2.0 2.0 31.0 2.0 34.0 82-83 14.485805185836742 2.0 2.0 30.0 2.0 34.0 84-85 13.96512108873404 2.0 2.0 30.0 2.0 34.0 86-87 13.407123805284417 2.0 2.0 29.0 2.0 34.0 88-89 12.862293101862509 2.0 2.0 29.0 2.0 34.0 90-91 12.28876635786613 2.0 2.0 29.0 2.0 34.0 92-93 11.667764770925086 2.0 2.0 27.0 2.0 34.0 94-95 11.017408331669154 2.0 2.0 26.0 2.0 33.0 96-97 10.299173449150048 2.0 2.0 25.0 2.0 33.0 98-99 9.514539845533672 2.0 2.0 21.0 2.0 32.0 100-101 8.499503795091002 2.0 2.0 2.0 2.0 31.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 7.11969143149393 1101 2 8.272717208970956 1101 3 7.759014410772924 1101 4 9.49684369804466 1101 5 9.488929185711786 1101 6 9.153701534061621 1101 7 8.809805336710244 1101 8 8.034195316439803 1101 9 8.299502792777162 1101 10-11 7.58422658066905 1101 12-13 7.525495244730568 1101 14-15 8.015621225114323 1101 16-17 8.706298701721327 1101 18-19 9.241769398358851 1101 20-21 9.885700480183662 1101 22-23 9.429051665521303 1101 24-25 9.604858300397343 1101 26-27 9.419328891123715 1101 28-29 9.361045508461007 1101 30-31 9.204723824970205 1101 32-33 9.312037946978645 1101 34-35 11.877553799827417 1101 36-37 5.800691515602914 1101 38-39 9.079757805063185 1101 40-41 10.612680582699145 1101 42-43 11.09641229811455 1101 44-45 5.1450227163498 1101 46-47 6.8886516124610395 1101 48-49 10.297513319351381 1101 50-51 -3.0052772647460486 1101 52-53 6.7903703041937895 1101 54-55 9.327850494835403 1101 56-57 9.97278404033446 1101 58-59 10.108814806897797 1101 60-61 9.85370884314828 1101 62-63 10.141128257175353 1101 64-65 10.077724413655183 1101 66-67 10.024633894010787 1101 68-69 9.723132237580405 1101 70-71 9.8702155854962 1101 72-73 9.930507688917057 1101 74-75 9.867396314443745 1101 76-77 9.645278899166803 1101 78-79 9.369096062903525 1101 80-81 9.314979777252722 1101 82-83 9.076540887452438 1101 84-85 8.920764970195647 1101 86-87 8.812787173911724 1101 88-89 8.708212533828691 1101 90-91 8.318083975291882 1101 92-93 8.500408018414282 1101 94-95 8.274469068618144 1101 96-97 7.851497972244735 1101 98-99 7.42280520719083 1101 100-101 7.0164694873491396 1102 1 6.744050750258463 1102 2 7.858866910883204 1102 3 7.547476028226566 1102 4 9.37411632449474 1102 5 9.425161326940557 1102 6 8.829938062195733 1102 7 8.341306028017517 1102 8 7.5506022602373015 1102 9 7.526383842742696 1102 10-11 6.966314944772332 1102 12-13 6.784388791625684 1102 14-15 7.172298234690757 1102 16-17 7.847878677716576 1102 18-19 8.293923870003749 1102 20-21 8.723484386484618 1102 22-23 8.453910384988564 1102 24-25 8.330535005190658 1102 26-27 8.41783167483695 1102 28-29 8.317344866280504 1102 30-31 8.081197936067351 1102 32-33 8.178834988528553 1102 34-35 10.477721471621173 1102 36-37 1.4124011024698042 1102 38-39 3.9137052229914957 1102 40-41 8.464212590266968 1102 42-43 9.76225210706236 1102 44-45 1.3275418022633154 1102 46-47 2.2786824111653416 1102 48-49 7.886147291250545 1102 50-51 -4.375873004847374 1102 52-53 4.995825757798979 1102 54-55 7.89431416444064 1102 56-57 8.480946230961106 1102 58-59 8.95781982555036 1102 60-61 8.72405936017054 1102 62-63 8.610786316799476 1102 64-65 8.83484430350607 1102 66-67 8.683786547636675 1102 68-69 8.65953278996901 1102 70-71 8.479056842779983 1102 72-73 8.306503265864936 1102 74-75 8.307704827258192 1102 76-77 8.154224407066108 1102 78-79 8.184328482626855 1102 80-81 8.094744395957532 1102 82-83 7.912408192602783 1102 84-85 7.657137015914758 1102 86-87 7.523493769632109 1102 88-89 7.454906129850572 1102 90-91 7.3216456659427624 1102 92-93 6.934492301216876 1102 94-95 6.5395326253468244 1102 96-97 6.386272001436554 1102 98-99 5.92022605189192 1102 100-101 5.383044308319862 1103 1 6.523533129553638 1103 2 7.253859510587194 1103 3 7.251168175912472 1103 4 8.661646225690792 1103 5 8.738247450385497 1103 6 8.089589248243175 1103 7 7.707836689243965 1103 8 6.979378611291349 1103 9 7.01719307511199 1103 10-11 6.527967010854976 1103 12-13 6.441327869188786 1103 14-15 6.835199950759396 1103 16-17 7.559176329622655 1103 18-19 7.888659259419324 1103 20-21 7.99877699818909 1103 22-23 7.961946306425421 1103 24-25 7.939383759140814 1103 26-27 8.0217362310192 1103 28-29 7.715557194773641 1103 30-31 7.515032489449485 1103 32-33 7.5628047473189035 1103 34-35 9.95246286127676 1103 36-37 -3.2264624520723757 1103 38-39 -1.8922566154820437 1103 40-41 6.231041663429892 1103 42-43 8.516378272108962 1103 44-45 0.5328260136317695 1103 46-47 0.4185232047970864 1103 48-49 6.942066728028017 1103 50-51 -5.162466068569923 1103 52-53 3.8355013448224646 1103 54-55 6.934598175801096 1103 56-57 7.307869307884182 1103 58-59 7.462136798229267 1103 60-61 7.457979916992812 1103 62-63 7.415108489686393 1103 64-65 7.259776100777962 1103 66-67 7.158535137580277 1103 68-69 7.273252138742961 1103 70-71 7.139070043308003 1103 72-73 7.3415122662249495 1103 74-75 7.127949237034581 1103 76-77 7.046781309342199 1103 78-79 6.811516770158354 1103 80-81 6.545222415078298 1103 82-83 6.419511276726148 1103 84-85 6.640075133439462 1103 86-87 6.337640335494745 1103 88-89 6.1765881971332615 1103 90-91 5.965579823309058 1103 92-93 5.851282972843739 1103 94-95 5.6602506540679745 1103 96-97 5.338151676623559 1103 98-99 4.7758298760448845 1103 100-101 4.271546248397465 1104 1 6.468745529094395 1104 2 7.436967049814136 1104 3 7.010869668449786 1104 4 8.707218364994272 1104 5 8.725549402165061 1104 6 7.8469792214540455 1104 7 7.633362904322421 1104 8 6.955207160851241 1104 9 6.946630503355394 1104 10-11 6.265785610166109 1104 12-13 6.177114321542408 1104 14-15 6.544055672152432 1104 16-17 7.278441542022197 1104 18-19 7.60366882696621 1104 20-21 8.019852390458514 1104 22-23 7.703381438457566 1104 24-25 7.904492829175258 1104 26-27 8.053033590376263 1104 28-29 7.877068867180839 1104 30-31 7.551117449265785 1104 32-33 7.21296930912526 1104 34-35 9.790801935138223 1104 36-37 -3.2483912695235837 1104 38-39 -1.9760759801969314 1104 40-41 5.916682689070917 1104 42-43 8.224127985082553 1104 44-45 0.4018271617373941 1104 46-47 -0.10658586523735636 1104 48-49 6.673999372497974 1104 50-51 -5.224758452802606 1104 52-53 3.590361658637999 1104 54-55 6.379309235885291 1104 56-57 6.841642748374042 1104 58-59 7.250142921459272 1104 60-61 7.010693273288258 1104 62-63 6.942567349234803 1104 64-65 7.0684481252708835 1104 66-67 6.695148225984408 1104 68-69 6.754962816125282 1104 70-71 6.755549186055806 1104 72-73 6.5787950829107515 1104 74-75 6.499790033054481 1104 76-77 6.464400903678209 1104 78-79 6.076533991742741 1104 80-81 5.975647214159812 1104 82-83 5.80973707083254 1104 84-85 5.709080108563841 1104 86-87 5.643476539092143 1104 88-89 5.512596616574518 1104 90-91 5.431014189937452 1104 92-93 5.088998242648563 1104 94-95 4.87126864105611 1104 96-97 4.97520487218422 1104 98-99 4.712232478417636 1104 100-101 4.27515512708097 1105 1 5.845254331702645 1105 2 6.999102894654108 1105 3 6.616198154846195 1105 4 8.44630997548823 1105 5 8.431344442519926 1105 6 7.48180170601713 1105 7 7.249198892103497 1105 8 6.333498252790669 1105 9 6.458961894527874 1105 10-11 5.856071419814999 1105 12-13 5.860968869899299 1105 14-15 5.9553555171181785 1105 16-17 6.605986969962704 1105 18-19 7.330031060523748 1105 20-21 7.598616445729075 1105 22-23 7.4033654754916505 1105 24-25 7.6786359686069225 1105 26-27 7.539894739177711 1105 28-29 7.314137776402351 1105 30-31 6.894697797403563 1105 32-33 6.454223417775008 1105 34-35 8.878816650090442 1105 36-37 -3.6485028893534572 1105 38-39 -2.3312456834550694 1105 40-41 5.260840848202342 1105 42-43 7.493669073998692 1105 44-45 -3.256826579764139 1105 46-47 -4.106535194913722 1105 48-49 4.651082720947752 1105 50-51 -9.507253972288309 1105 52-53 0.5107023470288148 1105 54-55 5.144430518146805 1105 56-57 6.241033737037917 1105 58-59 6.658940208722516 1105 60-61 6.370766025714747 1105 62-63 6.632237617243327 1105 64-65 6.395030454267612 1105 66-67 6.295821451978888 1105 68-69 6.183072389793157 1105 70-71 6.140465029783204 1105 72-73 6.063230102755622 1105 74-75 5.96818420705565 1105 76-77 5.814618338623076 1105 78-79 5.901694649320193 1105 80-81 5.697373294501906 1105 82-83 5.423869253864872 1105 84-85 5.465933179137076 1105 86-87 5.02897636132275 1105 88-89 4.88618008474654 1105 90-91 4.737245430429207 1105 92-93 4.5235720919243025 1105 94-95 4.0747713027597445 1105 96-97 3.716340226042055 1105 98-99 3.4720145113739047 1105 100-101 3.116754979060742 1106 1 5.93565208050283 1106 2 6.934585945350719 1106 3 6.514981775532064 1106 4 7.860352835296116 1106 5 7.717974580126892 1106 6 7.052381596171042 1106 7 6.9831978948476845 1106 8 6.116187565532542 1106 9 6.0531804923547625 1106 10-11 5.65825195178186 1106 12-13 5.5597294200867715 1106 14-15 5.888286018281988 1106 16-17 6.440699794270227 1106 18-19 6.83104230195929 1106 20-21 7.2687232288485895 1106 22-23 7.257904827219889 1106 24-25 7.131153392265649 1106 26-27 7.266108373379932 1106 28-29 6.975259085845007 1106 30-31 6.729881971873862 1106 32-33 6.763526792748511 1106 34-35 8.959955454275772 1106 36-37 -0.023036942726754717 1106 38-39 1.2867418848288672 1106 40-41 6.873125865485562 1106 42-43 7.764379296286876 1106 44-45 -3.343628867673326 1106 46-47 -4.114915472550292 1106 48-49 4.487012410020775 1106 50-51 -9.462040303179823 1106 52-53 0.3814412109477807 1106 54-55 4.886401094708152 1106 56-57 5.965254362202192 1106 58-59 6.369115107604152 1106 60-61 6.249959140812262 1106 62-63 6.220560408191094 1106 64-65 6.28338449903686 1106 66-67 5.980615681857682 1106 68-69 5.814637339372101 1106 70-71 5.6940694946412655 1106 72-73 5.607795231951567 1106 74-75 5.466926888009379 1106 76-77 5.38218750561858 1106 78-79 5.289629356573366 1106 80-81 5.193673345982864 1106 82-83 4.936345469637374 1106 84-85 4.7270508823695625 1106 86-87 4.620611548779793 1106 88-89 4.701474623118122 1106 90-91 4.498525066368424 1106 92-93 4.333657968271519 1106 94-95 4.019878658018376 1106 96-97 3.6843077174443657 1106 98-99 3.6142424002776643 1106 100-101 3.1910290385507256 1107 1 5.845909328418383 1107 2 7.09849006451164 1107 3 6.540417539780158 1107 4 7.887549533578831 1107 5 8.285222885891468 1107 6 6.628024852079541 1107 7 6.957684996153343 1107 8 6.418617209842921 1107 9 6.460217150273472 1107 10-11 5.866836652663547 1107 12-13 5.6680366184547815 1107 14-15 5.9103335385456575 1107 16-17 6.819557764345692 1107 18-19 7.070535115751294 1107 20-21 7.468924776619314 1107 22-23 7.471588114998529 1107 24-25 7.315066867381173 1107 26-27 7.1265749959438445 1107 28-29 7.203281553748003 1107 30-31 7.141101925738386 1107 32-33 6.574106371902971 1107 34-35 8.924480868714618 1107 36-37 -0.09463381633153034 1107 38-39 1.089918712370764 1107 40-41 6.963979126729953 1107 42-43 8.146572537131473 1107 44-45 -3.1633645324633566 1107 46-47 -4.046994509041237 1107 48-49 4.614402556957359 1107 50-51 -9.505161067591258 1107 52-53 0.3893364886862294 1107 54-55 5.298369706433309 1107 56-57 6.414867986686296 1107 58-59 6.607272588012002 1107 60-61 6.523352543729942 1107 62-63 6.447019088629432 1107 64-65 6.343577725362028 1107 66-67 6.159135303953342 1107 68-69 6.0112913832135 1107 70-71 6.084441936046307 1107 72-73 5.8875445132400195 1107 74-75 6.019014758663083 1107 76-77 5.873408584738554 1107 78-79 5.726648400614414 1107 80-81 5.8391033115355295 1107 82-83 5.647180341122311 1107 84-85 5.471477805195187 1107 86-87 5.25258699824675 1107 88-89 5.105500655539997 1107 90-91 4.958426270754741 1107 92-93 4.797632552017744 1107 94-95 4.642378027330844 1107 96-97 4.304751794063373 1107 98-99 4.136474816346315 1107 100-101 3.7974691295875225 1108 1 5.879583477416066 1108 2 6.174842867275249 1108 3 6.668202372141298 1108 4 7.753317686221312 1108 5 8.110673637174102 1108 6 7.342706243105134 1108 7 7.432172308881601 1108 8 6.672287626213784 1108 9 5.057805214650308 1108 10-11 6.1344394645757525 1108 12-13 5.467906525431875 1108 14-15 6.121479912408489 1108 16-17 6.1321096826231205 1108 18-19 6.701786579526509 1108 20-21 7.626830443625085 1108 22-23 7.776940775044043 1108 24-25 7.18660503897954 1108 26-27 7.2836845064574085 1108 28-29 7.45768591616411 1108 30-31 6.95865812555834 1108 32-33 7.339514435840673 1108 34-35 9.724197994152831 1108 36-37 -3.415184580056252 1108 38-39 -2.2068688041881934 1108 40-41 5.727548780473924 1108 42-43 8.136404331061197 1108 44-45 -2.9762012007645726 1108 46-47 -4.012795230682425 1108 48-49 4.9096370994910075 1108 50-51 -9.560278837456519 1108 52-53 0.6712525064125501 1108 54-55 5.458601175038989 1108 56-57 6.56340068925449 1108 58-59 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3.9820040627641298 1109 20-21 4.25700321641531 1109 22-23 5.837793792272901 1109 24-25 5.5193952291522805 1109 26-27 4.503338702621672 1109 28-29 4.611275180491628 1109 30-31 4.532981577398573 1109 32-33 4.062942387456548 1109 34-35 6.0871104359243375 1109 36-37 -4.726542002151929 1109 38-39 -3.2335627167881444 1109 40-41 3.38407085645909 1109 42-43 3.9359142916449805 1109 44-45 -4.498853418047661 1109 46-47 -5.756625350351468 1109 48-49 2.2266010825623717 1109 50-51 -9.810191676295531 1109 52-53 -0.9174549581338418 1109 54-55 2.4153070965100163 1109 56-57 3.7712300212448966 1109 58-59 4.667388583481053 1109 60-61 5.074310253323151 1109 62-63 5.088888413466314 1109 64-65 4.627056598058459 1109 66-67 4.664065903111041 1109 68-69 4.735511731002553 1109 70-71 4.905695639933601 1109 72-73 4.759894994607681 1109 74-75 4.875861694947041 1109 76-77 4.837927570488459 1109 78-79 4.89828777692936 1109 80-81 4.771644141500024 1109 82-83 4.672642959857832 1109 84-85 4.601176624540949 1109 86-87 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-12.723325640698787 2313 82-83 -12.267962320992503 2313 84-85 -11.73959498908646 2313 86-87 -11.1793272084285 2313 88-89 -10.64162708114799 2313 90-91 -10.060355071202652 2313 92-93 -9.455747163215614 2313 94-95 -8.945646781829462 2313 96-97 -8.205894578723356 2313 98-99 -7.388173362312736 2313 100-101 -6.367868913882919 2314 1 -20.478518152497646 2314 2 -19.42870459197691 2314 3 -19.815498490754194 2314 4 -21.711687107642543 2314 5 -21.703803831665784 2314 6 -22.45451331585939 2314 7 -22.52036843896116 2314 8 -23.29703164511891 2314 9 -24.39665307873416 2314 10-11 -24.65510991222195 2314 12-13 -24.58482597696506 2314 14-15 -24.7848000492406 2314 16-17 -23.928772388326063 2314 18-19 -23.30852022776016 2314 20-21 -22.82019736078527 2314 22-23 -22.72497677049766 2314 24-25 -22.437795728038676 2314 26-27 -22.096468897185925 2314 28-29 -22.015468446251997 2314 30-31 -21.901121356704362 2314 32-33 -21.590015765501608 2314 34-35 -18.8465114976976 2314 36-37 -17.22774450335443 2314 38-39 -16.35020533343076 2314 40-41 -17.616650644262414 2314 42-43 -17.532339676608988 2314 44-45 -15.605635524829768 2314 46-47 -16.380963974690093 2314 48-49 -15.871523015561728 2314 50-51 -13.907594273698129 2314 52-53 -16.76834480902369 2314 54-55 -18.325914644711727 2314 56-57 -18.519053769038894 2314 58-59 -18.15632474023227 2314 60-61 -17.826122647109752 2314 62-63 -17.373353048775147 2314 64-65 -16.88304441204255 2314 66-67 -16.473259734214597 2314 68-69 -15.99777350228268 2314 70-71 -15.408878674640714 2314 72-73 -14.942846708134024 2314 74-75 -14.415384096298752 2314 76-77 -13.846295613734727 2314 78-79 -13.352072973431389 2314 80-81 -12.8522134823576 2314 82-83 -12.40792462070975 2314 84-85 -11.88992486656054 2314 86-87 -11.325180177325768 2314 88-89 -10.775463084918018 2314 90-91 -10.209035561306328 2314 92-93 -9.593588822028055 2314 94-95 -8.945646781829462 2314 96-97 -8.237232938761055 2314 98-99 -7.4713496111346025 2314 100-101 -6.4658896490384326 2315 1 -20.478518152497646 2315 2 -19.42870459197691 2315 3 -19.815498490754194 2315 4 -16.769815652065983 2315 5 -21.703803831665784 2315 6 -22.45451331585939 2315 7 -22.52036843896116 2315 8 -23.29703164511891 2315 9 -19.787957426560247 2315 10-11 -18.17614015796864 2315 12-13 -14.89342711118056 2315 14-15 -14.66854296039372 2315 16-17 -20.05282720874194 2315 18-19 -23.30852022776016 2315 20-21 -22.82019736078527 2315 22-23 -22.72497677049766 2315 24-25 -22.437795728038676 2315 26-27 -22.096468897185925 2315 28-29 -22.015468446251997 2315 30-31 -21.901121356704362 2315 32-33 -21.590015765501608 2315 34-35 -18.8465114976976 2315 36-37 -17.22774450335443 2315 38-39 -16.35020533343076 2315 40-41 -17.616650644262414 2315 42-43 -17.532339676608988 2315 44-45 -15.605635524829768 2315 46-47 -16.380963974690093 2315 48-49 -15.871523015561728 2315 50-51 -13.907594273698129 2315 52-53 -16.76834480902369 2315 54-55 -18.325914644711727 2315 56-57 -18.519053769038894 2315 58-59 -18.15632474023227 2315 60-61 -17.826122647109752 2315 62-63 -17.373353048775147 2315 64-65 -16.88304441204255 2315 66-67 -16.473259734214597 2315 68-69 -15.99777350228268 2315 70-71 -15.408878674640714 2315 72-73 -14.942846708134024 2315 74-75 -14.415384096298752 2315 76-77 -13.846295613734727 2315 78-79 -13.352072973431389 2315 80-81 -12.8522134823576 2315 82-83 -12.40792462070975 2315 84-85 -11.88992486656054 2315 86-87 -11.325180177325768 2315 88-89 -10.775463084918018 2315 90-91 -10.209035561306328 2315 92-93 -9.593588822028055 2315 94-95 -8.945646781829462 2315 96-97 -8.237232938761055 2315 98-99 -7.4713496111346025 2315 100-101 -6.4658896490384326 2316 1 -20.478518152497646 2316 2 -19.42870459197691 2316 3 -19.815498490754194 2316 4 -21.711687107642543 2316 5 -21.703803831665784 2316 6 -22.45451331585939 2316 7 -22.52036843896116 2316 8 -23.29703164511891 2316 9 -24.39665307873416 2316 10-11 -24.65510991222195 2316 12-13 -24.58482597696506 2316 14-15 -24.7848000492406 2316 16-17 -23.928772388326063 2316 18-19 -23.30852022776016 2316 20-21 -22.82019736078527 2316 22-23 -22.72497677049766 2316 24-25 -22.437795728038676 2316 26-27 -22.096468897185925 2316 28-29 -22.015468446251997 2316 30-31 -21.901121356704362 2316 32-33 -21.590015765501608 2316 34-35 -18.8465114976976 2316 36-37 -17.22774450335443 2316 38-39 -16.35020533343076 2316 40-41 -17.616650644262414 2316 42-43 -17.532339676608988 2316 44-45 -15.605635524829768 2316 46-47 -16.380963974690093 2316 48-49 -15.871523015561728 2316 50-51 -13.907594273698129 2316 52-53 -16.76834480902369 2316 54-55 -18.325914644711727 2316 56-57 -18.519053769038894 2316 58-59 -18.15632474023227 2316 60-61 -17.826122647109752 2316 62-63 -17.373353048775147 2316 64-65 -16.88304441204255 2316 66-67 -16.473259734214597 2316 68-69 -15.99777350228268 2316 70-71 -15.408878674640714 2316 72-73 -14.942846708134024 2316 74-75 -14.415384096298752 2316 76-77 -13.846295613734727 2316 78-79 -13.352072973431389 2316 80-81 -12.8522134823576 2316 82-83 -12.40792462070975 2316 84-85 -11.88992486656054 2316 86-87 -11.325180177325768 2316 88-89 -10.775463084918018 2316 90-91 -10.209035561306328 2316 92-93 -9.593588822028055 2316 94-95 -8.945646781829462 2316 96-97 -8.237232938761055 2316 98-99 -7.4713496111346025 2316 100-101 -6.4658896490384326 >>END_MODULE >>Per sequence quality scores fail #Quality Count 2 342607.0 3 20954.0 4 42781.0 5 26063.0 6 23133.0 7 28103.0 8 35509.0 9 41730.0 10 43064.0 11 39791.0 12 35499.0 13 33126.0 14 32068.0 15 34398.0 16 36724.0 17 38430.0 18 40471.0 19 41776.0 20 43533.0 21 46024.0 22 48602.0 23 51698.0 24 54713.0 25 57453.0 26 60548.0 27 64840.0 28 68466.0 29 72452.0 30 74574.0 31 73938.0 32 71930.0 33 69983.0 34 65061.0 35 54093.0 36 37144.0 37 14930.0 38 1717.0 39 11.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.83007160189466 20.44483311296624 14.20033607225255 23.52475921288655 2 15.12209898861582 21.058679826061464 41.06897281803228 22.750248367290435 3 18.372056098843288 26.303329393819393 30.526125896816037 24.798488610521282 4 12.297620714340434 17.33118219352862 39.59323381560074 30.777963276530212 5 12.91360245629214 37.71365943214071 35.97404961119259 13.398688500374565 6 29.772433510289503 38.2353425575016 18.43552689447105 13.556697037737841 7 25.8397005312612 32.86339925397363 23.656455100131552 17.640445114633614 8 24.48878726615818 36.32422813541584 21.419216801590395 17.76776779683559 9 25.58969819437149 16.54135377798813 21.501361921708 36.367586105932375 10-11 24.19031897854493 26.717089448165098 29.530161840833895 19.56242973245607 12-13 24.72047490177671 24.492591071080387 29.464532363224187 21.32240166391871 14-15 21.942844587521428 25.951025379950337 27.21432343536012 24.891806597168113 16-17 21.665849508262912 28.861967525163674 27.72537709456926 21.746805872004153 18-19 21.579408305339985 27.936937845341475 29.616419067532014 20.867234781786525 20-21 22.24607062561521 27.144657620241354 29.671278872385752 20.937992881757687 22-23 21.917263196995698 27.08207105197758 29.46061256723161 21.54005318379511 24-25 21.801064691131597 27.21141477616594 29.422307822827314 21.565212709875144 26-27 21.822789402800886 27.348944824160842 29.26491119098137 21.563354582056903 28-29 21.85537019496432 27.512101223364244 29.259592454638394 21.372936127033043 30-31 22.085386463110122 27.402250847361074 29.137204847759833 21.375157841768967 32-33 21.947055591454173 27.490760394883505 29.04041536999498 21.52176864366734 34-35 22.22263701463356 27.424597857878414 29.04012642029052 21.31263870719751 36-37 22.681532898501438 27.445105248090673 28.556002183915126 21.31735966949277 38-39 22.529442445624912 27.482583692954744 28.59377232061287 21.39420154080748 40-41 22.468278627779142 27.387734184129336 28.736007665158976 21.40797952293255 42-43 22.509110849158915 27.440126810661308 28.493168384596583 21.557593955583187 44-45 22.793243056824362 27.62900743827574 28.13705078997234 21.440698714927557 46-47 22.158404816735096 27.594042810672004 28.419627410615362 21.827924961977537 48-49 22.68368233637031 27.633097015448143 27.97830423915774 21.704916409023806 50-51 22.837173288991956 27.836449626259025 27.732443390428053 21.59393369432097 52-53 21.890663599488153 27.95049831584173 28.30076181880465 21.858076265865474 54-55 21.747734720910227 27.816683172874235 28.437908807161172 21.99767329905437 56-57 21.80209900646095 28.010508440389113 28.24319700032005 21.944195552829893 58-59 21.855066648571142 27.922800951195192 28.358096665506196 21.864035734727473 60-61 21.809261510965268 27.982479014371307 28.310836348720354 21.89742312594307 62-63 21.81283552452386 27.895344171647977 28.303084262966294 21.988736040861873 64-65 21.639059292570927 27.743542128931974 28.37992572113388 22.23747285736322 66-67 21.737139620991524 27.881200519113232 28.1977386478868 22.183921212008446 68-69 21.760235019840103 27.876755878439635 28.194903978617436 22.168105123102826 70-71 21.69172318425661 27.692229294641947 28.164985036736432 22.45106248436501 72-73 21.84335034008229 27.765699438354822 28.038460753667305 22.35248946789558 74-75 21.828172862587188 27.935830876536993 27.92239879197527 22.313597468900554 76-77 21.84461967459232 27.805466904298086 27.952864011273675 22.397049409835915 78-79 21.830754957295706 27.8692507111598 27.819131153021694 22.4808631785228 80-81 21.865528928530328 27.947948248952308 27.75902558233807 22.42749724017929 82-83 21.98991262342829 27.842494849754917 27.7893301129502 22.37826241386659 84-85 21.994505779886637 27.869103710996868 27.7072969702887 22.4290935388278 86-87 21.868418718472395 27.86423626431109 27.702106464950628 22.565238552265892 88-89 21.958973600122082 27.754756047370876 27.55915001549126 22.727120337015783 90-91 21.992211305873692 27.723804249057434 27.578478546282604 22.705505898786278 92-93 22.00743453830402 27.63790763953984 27.619143131529146 22.735514690627 94-95 21.907994042750772 27.530845622725003 27.6043187467798 22.95684158774443 96-97 21.90216243533384 27.479491976788573 27.535351407903768 23.08299417997382 98-99 21.88921800490941 27.426032451937843 27.429688943939407 23.25506059921334 100-101 22.246863609634662 27.646740510745982 27.28353586685185 22.822860012767507 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 24797.0 1 26641.0 2 19872.5 3 11908.0 4 15350.5 5 19455.0 6 20514.0 7 19450.0 8 17818.0 9 16127.5 10 14293.0 11 12427.5 12 10661.0 13 9143.5 14 7859.0 15 6705.5 16 5934.5 17 5637.0 18 5625.5 19 5841.0 20 6384.5 21 7137.5 22 8125.0 23 9505.5 24 11140.5 25 13209.5 26 15877.0 27 18952.0 28 22382.0 29 26205.0 30 30327.5 31 34672.5 32 38964.5 33 42778.0 34 46050.5 35 49285.5 36 52649.0 37 55584.0 38 57922.5 39 59489.0 40 60503.0 41 61694.0 42 62691.5 43 63348.0 44 63715.0 45 63699.0 46 63212.5 47 62178.5 48 60760.0 49 58953.0 50 56253.0 51 52920.5 52 49845.0 53 46322.5 54 42381.0 55 38366.0 56 33885.0 57 29397.5 58 25467.5 59 21549.0 60 17616.0 61 14371.5 62 11533.5 63 8951.0 64 6865.5 65 5242.5 66 3991.0 67 3009.5 68 2304.0 69 1756.5 70 1312.5 71 991.5 72 721.0 73 533.0 74 404.5 75 295.5 76 201.5 77 136.0 78 93.5 79 58.0 80 36.5 81 24.0 82 15.0 83 8.0 84 4.5 85 4.0 86 2.5 87 1.5 88 2.5 89 2.5 90 1.0 91 0.5 92 0.5 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 14.873545240523454 2 21.99826518836731 3 17.749247054148583 4 23.054650631600502 5 22.94306169353999 6 16.695046640212567 7 15.4844895949413 8 12.505481628731003 9 3.1593491051796883 10-11 6.757635025918004 12-13 3.704005768477345 14-15 7.7226049411134605 16-17 11.90520834762495 18-19 15.084603826240372 20-21 15.492670751147013 22-23 13.407263545530167 24-25 15.995506969989384 26-27 13.446517850927139 28-29 13.46638637314101 30-31 14.365551336247046 32-33 12.789230549555194 34-35 19.690391511516882 36-37 24.47311575522997 38-39 23.52117979386535 40-41 18.645236102578487 42-43 18.59122014576686 44-45 23.93458225542789 46-47 13.248518626358466 48-49 22.02059822036986 50-51 21.70618266743295 52-53 11.66355935174754 54-55 13.339756303174338 56-57 12.833439281846928 58-59 13.883930227441224 60-61 13.283555317065538 62-63 13.248493219041057 64-65 14.349900428723075 66-67 12.606145420305628 68-69 13.68727759069523 70-71 12.248410391186303 72-73 11.592723750811128 74-75 11.665109198109493 76-77 11.727662013570557 78-79 12.148305560594673 80-81 12.127319116414805 82-83 10.585298208225161 84-85 10.481407687339585 86-87 10.628439833185716 88-89 12.09027524763242 90-91 9.49042067911727 92-93 11.583196006782739 94-95 11.681598547107962 96-97 11.214485016542703 98-99 11.058560309603408 100-101 11.166465186639613 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1967937.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 98.02544862405246 #Duplication Level Percentage of deduplicated Percentage of total 1 99.03978433954225 97.08419291513034 2 0.7135992199514181 1.3990176734702329 3 0.134944787025432 0.39684069862935556 4 0.04739310450955417 0.18582921324942606 5 0.024051017075953363 0.11788058693675371 6 0.010179695361003403 0.05987215227711265 7 0.0052319588473669605 0.03590055792370087 8 0.004994767941683348 0.03916914946052362 9 0.0035843738384463424 0.03162238681950177 >10 0.014859583289034283 0.2327651731071 >50 8.558620726334309E-4 0.0633190911794188 >100 3.6510128772132707E-4 0.05253450351913601 >500 0.0 0.0 >1k 1.561892574909149E-4 0.3010558982973706 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 3533 0.17952810481229836 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 5.081463481808615E-5 0.0 9 0.0 0.0 0.0 5.081463481808615E-5 0.0 10-11 0.0 0.0 0.0 5.081463481808615E-5 0.0 12-13 0.0 0.0 0.0 5.081463481808615E-5 1.016292696361723E-4 14-15 0.0 0.0 0.0 5.081463481808615E-5 1.016292696361723E-4 16-17 0.0 0.0 0.0 5.081463481808615E-5 1.016292696361723E-4 18-19 0.0 0.0 0.0 5.081463481808615E-5 1.5244390445425844E-4 20-21 0.0 0.0 0.0 1.016292696361723E-4 2.032585392723446E-4 22-23 0.0 0.0 0.0 2.0325853927234458E-4 2.032585392723446E-4 24-25 0.0 0.0 0.0 4.065170785446892E-4 2.032585392723446E-4 26-27 0.0 0.0 0.0 6.097756178170338E-4 2.032585392723446E-4 28-29 0.0 0.0 0.0 0.001727697583814929 2.032585392723446E-4 30-31 0.0 0.0 0.0 0.005284722021080959 2.032585392723446E-4 32-33 0.0 0.0 0.0 0.014736244097244982 2.032585392723446E-4 34-35 0.0 0.0 0.0 0.027998863784765467 2.032585392723446E-4 36-37 0.0 0.0 0.0 0.05053515432658667 2.032585392723446E-4 38-39 0.0 0.0 0.0 0.08018549374293994 2.032585392723446E-4 40-41 0.0 0.0 0.0 0.11039479414229215 2.032585392723446E-4 42-43 0.0 0.0 0.0 0.14418652629631945 2.032585392723446E-4 44-45 0.0 0.0 0.0 0.18443171707224368 2.032585392723446E-4 46-47 0.0 0.0 0.0 0.23443331773324044 2.032585392723446E-4 48-49 0.0 0.0 0.0 0.28852549649709314 2.032585392723446E-4 50-51 0.0 0.0 0.0 0.3513323851322476 2.032585392723446E-4 52-53 0.0 0.0 0.0 0.43250876425414025 2.540731740904307E-4 54-55 0.0 0.0 0.0 0.5202910459023841 3.048878089085169E-4 56-57 0.0 0.0 0.0 0.6207515789377404 3.048878089085169E-4 58-59 0.0 0.0 0.0 0.73147666820635 3.048878089085169E-4 60-61 0.0 0.0 0.0 0.8524663137082131 3.048878089085169E-4 62-63 0.0 0.0 0.0 0.9742181787323476 3.048878089085169E-4 64-65 0.0 0.0 0.0 1.098205887688478 3.048878089085169E-4 66-67 0.0 0.0 0.0 1.2349226626665386 3.048878089085169E-4 68-69 0.0 0.0 0.0 1.384444725618757 3.048878089085169E-4 70-71 0.0 0.0 0.0 1.5482457009548578 3.048878089085169E-4 72-73 0.0 0.0 0.0 1.7160610324415875 3.048878089085169E-4 74-75 0.0 0.0 0.0 1.8863916883518121 3.048878089085169E-4 76-77 0.0 0.0 0.0 2.0707471834718287 3.5570244372660305E-4 78-79 0.0 0.0 0.0 2.2735483910308103 3.5570244372660305E-4 80-81 0.0 0.0 0.0 2.4948461256635754 3.5570244372660305E-4 82-83 0.0 0.0 0.0 2.7199803652251062 3.5570244372660305E-4 84-85 0.0 0.0 0.0 2.943412314520231 3.5570244372660305E-4 86-87 0.0 0.0 0.0 3.1749491980688402 3.8110976113564613E-4 88-89 0.0 0.0 0.0 3.4267865282272756 4.065170785446892E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1535 0.0 55.253254 1 GTATCAA 3000 0.0 47.960155 1 ATCAACG 3800 0.0 37.58334 3 TCAACGC 3825 0.0 37.46061 4 TATCAAC 3885 0.0 36.503773 2 CAACGCA 3880 0.0 35.55635 5 AACGCAG 4135 0.0 33.713825 6 ACGCAGA 4785 0.0 28.295004 7 CGCAGAG 4905 0.0 27.758074 8 GTACATG 3610 0.0 26.430874 1 GCAGAGT 5270 0.0 24.28857 9 TACATGG 3660 0.0 24.269117 2 ACATGGG 3770 0.0 23.69324 3 CAGAGTA 5045 0.0 20.053957 10-11 CATGGGG 2790 0.0 19.530237 4 GAGTACA 2705 0.0 19.409887 1 AGAGTAC 4925 0.0 19.10729 10-11 GAGTACT 3715 0.0 18.762194 12-13 AGTACAT 2845 0.0 18.09424 2 GTATTAG 605 0.0 17.5235 1 >>END_MODULE