FastQCFastQC Report
Sat 18 Jun 2016
SRR1930904_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1930904_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1967937
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40570.2061549734569755No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33630.1708896168932237No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22290.11326582100951402No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA22700.052.0930371
GTATCAA40550.041.6932071
ATCAACG51900.031.6490253
TCAACGC52400.031.6156124
CAACGCA53100.031.1007485
TATCAAC55250.029.9893362
AACGCAG55450.029.7826796
ATAACGC2201.8189894E-1228.0525463
TAACGCA1806.7520887E-926.3715084
ACGCAGA66150.024.8217457
CGCAGAG67450.024.2729878
GTGGTAT11750.022.6337411
GTACATG45000.022.0566731
TATATCG1754.0847644E-621.6939185
TACATGG46100.020.9058952
GCAGAGT78750.020.5489649
AGAGTAC72550.020.24460410-11
ACATGGG46800.019.8820823
TATAACG2651.8675564E-819.7072
GAGTACT54400.018.319212-13
Produced by FastQC (version 0.11.5)