##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1930904_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1967937 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.246829547897114 31.0 31.0 33.0 30.0 34.0 2 31.362423187327643 31.0 31.0 34.0 30.0 34.0 3 31.440979055731965 31.0 31.0 34.0 28.0 34.0 4 35.30726491752531 37.0 35.0 37.0 33.0 37.0 5 35.09084437154238 37.0 35.0 37.0 32.0 37.0 6 35.13123997363737 37.0 35.0 37.0 32.0 37.0 7 35.01052574345622 36.0 35.0 37.0 32.0 37.0 8 34.99158306388873 36.0 35.0 37.0 32.0 37.0 9 36.49969434997157 38.0 35.0 39.0 32.0 39.0 10-11 36.43031712905444 38.0 35.0 39.0 32.0 39.0 12-13 36.327120736080474 38.0 35.0 39.0 32.0 39.0 14-15 37.33567283912036 39.0 36.0 41.0 32.0 41.0 16-17 37.30544143435486 39.0 36.0 41.0 32.0 41.0 18-19 37.30503415505679 39.0 36.0 40.5 32.0 41.0 20-21 37.27166393029859 39.0 36.0 40.0 31.0 41.0 22-23 37.145251601042105 39.0 36.0 40.0 31.0 41.0 24-25 37.02853470410892 39.0 36.0 40.0 31.0 41.0 26-27 36.747546288321224 39.0 35.5 40.0 30.0 41.0 28-29 36.5560912264976 38.0 35.0 40.0 30.0 41.0 30-31 36.32220899347896 38.0 35.0 40.0 30.0 41.0 32-33 36.077071572921284 38.0 34.5 40.0 30.0 41.0 34-35 36.25663143688035 38.0 35.0 40.0 30.0 41.0 36-37 36.297809330278355 38.0 35.0 40.0 30.0 41.0 38-39 36.25786496214056 38.0 35.0 40.0 30.0 41.0 40-41 36.162348693073 38.0 35.0 40.0 30.0 41.0 42-43 36.02100245078984 38.0 35.0 40.0 29.5 41.0 44-45 35.83384808558404 38.0 35.0 40.0 28.5 41.0 46-47 35.6104771138507 38.0 34.0 40.0 28.0 41.0 48-49 35.41060409962311 38.0 34.0 40.0 27.0 41.0 50-51 35.14515937247991 38.0 34.0 40.0 26.5 41.0 52-53 34.94023970279537 38.0 33.5 40.0 26.0 41.0 54-55 34.77822282928773 38.0 33.0 40.0 26.0 41.0 56-57 34.52727069006782 38.0 33.0 40.0 26.0 41.0 58-59 34.293915404812246 37.0 33.0 40.0 25.0 41.0 60-61 33.98746021849277 37.0 32.5 40.0 24.5 41.0 62-63 33.689333042673624 36.5 32.0 39.5 23.5 41.0 64-65 33.38311566884509 36.0 32.0 39.0 23.0 41.0 66-67 33.046042124315974 36.0 31.0 39.0 22.5 40.0 68-69 32.67677674641007 35.0 31.0 39.0 22.0 40.0 70-71 32.28224201282866 35.0 31.0 38.0 21.5 40.0 72-73 31.862959535798147 35.0 30.5 37.5 20.5 40.0 74-75 31.34488375390066 34.5 30.0 37.0 19.0 39.0 76-77 29.96640085531193 33.5 28.5 35.5 18.0 38.5 78-79 30.725011522218445 34.0 30.0 36.0 18.5 39.0 80-81 30.710349721561208 34.0 30.0 36.0 18.5 37.5 82-83 30.419592954449254 34.0 30.0 35.0 18.0 37.0 84-85 30.089442395767747 34.0 30.0 35.0 17.5 37.0 86-87 29.73779292731424 34.0 29.0 35.0 12.5 36.0 88-89 29.388194337521984 34.0 29.0 35.0 8.5 36.0 90-91 29.034645926165318 34.0 29.0 35.0 4.5 35.5 92-93 28.668822731621997 34.0 29.0 35.0 2.0 35.0 94-95 28.251118811222106 33.0 29.0 35.0 2.0 35.0 96-97 27.783261354403113 33.0 27.0 35.0 2.0 35.0 98-99 27.2874418744096 33.0 27.0 35.0 2.0 35.0 100-101 26.071753567314403 32.0 24.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.5938937036364251 1101 2 0.7210345920665198 1101 3 0.6752514519975286 1101 4 0.5053534189798157 1101 5 0.6459624562127431 1101 6 0.5875427878195367 1101 7 0.6786019418991671 1101 8 0.6427121486973704 1101 9 0.8866449623262156 1101 10-11 0.777529710152919 1101 12-13 0.9307010849386259 1101 14-15 0.9107178439562418 1101 16-17 0.9874912810548082 1101 18-19 0.9336799047962998 1101 20-21 0.9155958860869049 1101 22-23 0.9442154594173999 1101 24-25 0.9290618309619205 1101 26-27 0.8550178490565372 1101 28-29 0.8323230091029998 1101 30-31 0.9437748621413178 1101 32-33 0.9566848631381717 1101 34-35 0.8041774758139582 1101 36-37 0.9797903261236982 1101 38-39 1.0714105382115946 1101 40-41 1.1922869548031585 1101 42-43 1.2316607060820957 1101 44-45 1.1847460785489545 1101 46-47 1.0610127451244722 1101 48-49 1.0711258554086598 1101 50-51 1.1783323845556808 1101 52-53 1.0521495209505503 1101 54-55 1.101515271511353 1101 56-57 1.2753671925378924 1101 58-59 1.155703194986664 1101 60-61 1.148706573497165 1101 62-63 1.0151106199612414 1101 64-65 1.13167728522901 1101 66-67 1.262643041269321 1101 68-69 1.1648251803496237 1101 70-71 1.1743523074969318 1101 72-73 1.261441219132866 1101 74-75 1.3053750680993836 1101 76-77 1.4594517131505853 1101 78-79 1.4735345412868703 1101 80-81 1.3316142131903277 1101 82-83 1.5036334174824546 1101 84-85 1.5040781607752294 1101 86-87 1.3300242809093348 1101 88-89 1.3085254778694804 1101 90-91 1.3886773588405141 1101 92-93 1.5821837513099872 1101 94-95 1.531248958876585 1101 96-97 1.5056995697887672 1101 98-99 1.6928114102529044 1101 100-101 2.0811266923193656 1102 1 0.46125225263873304 1102 2 0.5209136646385879 1102 3 0.5548719764709595 1102 4 0.28838891101561615 1102 5 0.40563480203232416 1102 6 0.3909691744800554 1102 7 0.5265521316287192 1102 8 0.5636418768817535 1102 9 0.6962787378826079 1102 10-11 0.5836679272455783 1102 12-13 0.5409372795364931 1102 14-15 0.6977015507926723 1102 16-17 0.7996573853925 1102 18-19 0.6963606489928935 1102 20-21 0.7426495369533157 1102 22-23 0.7839484168241668 1102 24-25 0.7662940880247149 1102 26-27 0.848851946812232 1102 28-29 0.8886935103685474 1102 30-31 0.7848707176659033 1102 32-33 0.8376760901490243 1102 34-35 0.768823455018051 1102 36-37 0.876544837694361 1102 38-39 0.9611920062475647 1102 40-41 0.8533586741332257 1102 42-43 0.9169902045700908 1102 44-45 1.0178949811243356 1102 46-47 1.0592546969194458 1102 48-49 0.9452701418752198 1102 50-51 0.7615845930010536 1102 52-53 0.9609211956721992 1102 54-55 0.9923704633809578 1102 56-57 0.6906354304396061 1102 58-59 0.7895839056653315 1102 60-61 0.9707263737204599 1102 62-63 0.8475653399509895 1102 64-65 0.9659746697626233 1102 66-67 1.0372982296885525 1102 68-69 0.8930970600399775 1102 70-71 0.8052480330573957 1102 72-73 0.9200693336097352 1102 74-75 1.085086288143767 1102 76-77 1.0344152172199728 1102 78-79 1.1354552993178153 1102 80-81 1.0152345437855388 1102 82-83 1.3147783751291051 1102 84-85 1.3807786000408235 1102 86-87 1.2234241243280337 1102 88-89 1.2675954046475937 1102 90-91 1.2835029464927707 1102 92-93 1.251826777157401 1102 94-95 1.2455353186406164 1102 96-97 1.226578610321635 1102 98-99 1.3250371732170727 1102 100-101 1.6142819205822079 1103 1 0.36689487834376067 1103 2 0.48211451267250993 1103 3 0.35407674466168615 1103 4 0.22184144911713588 1103 5 0.3221450624427433 1103 6 0.37977432668614597 1103 7 0.4048416632099787 1103 8 0.37861687588171833 1103 9 0.49983328006429417 1103 10-11 0.4115338418821679 1103 12-13 0.4487607924770103 1103 14-15 0.5289412003786538 1103 16-17 0.47685167316401333 1103 18-19 0.446551456625194 1103 20-21 0.5039747899634364 1103 22-23 0.4808314921471748 1103 24-25 0.5547964281183155 1103 26-27 0.6398851881418892 1103 28-29 0.7444581409537747 1103 30-31 0.7163879783563303 1103 32-33 0.7588633376389211 1103 34-35 0.7943852774909459 1103 36-37 0.8808994118773299 1103 38-39 0.6427788697221004 1103 40-41 0.6376456456120891 1103 42-43 0.5488790801251113 1103 44-45 0.7072121538112413 1103 46-47 0.6070722096199574 1103 48-49 0.5521932187983012 1103 50-51 0.5774412272664264 1103 52-53 0.5402363682791034 1103 54-55 0.6244285457042409 1103 56-57 0.6152732159510279 1103 58-59 0.5888566765761638 1103 60-61 0.4231712715163667 1103 62-63 0.5720835206782198 1103 64-65 0.5400116312410788 1103 66-67 0.37291565438553675 1103 68-69 0.5063915918212487 1103 70-71 0.545962061618539 1103 72-73 0.5287624813356508 1103 74-75 0.6958263177449489 1103 76-77 0.7633383741462509 1103 78-79 0.8957045092862153 1103 80-81 0.7952829457216168 1103 82-83 0.7989949437918504 1103 84-85 0.7961752159054569 1103 86-87 0.7727892989350167 1103 88-89 0.6672725917350775 1103 90-91 0.8793339797340991 1103 92-93 0.6933568383598505 1103 94-95 0.7106871247128588 1103 96-97 0.7391455129977444 1103 98-99 0.7749647703209597 1103 100-101 0.9401893568796638 1104 1 0.43278848722244234 1104 2 0.542688565485367 1104 3 0.5043484629931072 1104 4 0.24918167108422296 1104 5 0.404012647593909 1104 6 0.4414352528246823 1104 7 0.4125263168647848 1104 8 0.42847527618787495 1104 9 0.537904462613092 1104 10-11 0.5779364442549237 1104 12-13 0.5812866248535826 1104 14-15 0.623881881587991 1104 16-17 0.6280342219585009 1104 18-19 0.6999421952398208 1104 20-21 0.6980084677284566 1104 22-23 0.6860898159129576 1104 24-25 0.5934531445362268 1104 26-27 0.8386490610848654 1104 28-29 0.7265591742488411 1104 30-31 0.7079111318197917 1104 32-33 0.9384576736511718 1104 34-35 0.8494216341690972 1104 36-37 0.7564371080120438 1104 38-39 0.7570038984247489 1104 40-41 0.9797505059258995 1104 42-43 0.8448683644726032 1104 44-45 0.9320954297392916 1104 46-47 0.7990048540899153 1104 48-49 0.9494989975966135 1104 50-51 0.9436295165890414 1104 52-53 0.9866963759331426 1104 54-55 1.0362004553866058 1104 56-57 1.0058243066513697 1104 58-59 0.9037284714479696 1104 60-61 0.8400331161394021 1104 62-63 0.7960176959556762 1104 64-65 0.988959201084171 1104 66-67 0.9881089188325305 1104 68-69 1.0394378987481545 1104 70-71 0.9825215717601452 1104 72-73 1.251556205024901 1104 74-75 1.2735798577372925 1104 76-77 1.2094348150188132 1104 78-79 1.1247362735418598 1104 80-81 0.9287081275050078 1104 82-83 1.0604913081240355 1104 84-85 1.1807331952395543 1104 86-87 1.119532505976732 1104 88-89 1.082921271413607 1104 90-91 1.1167392610199762 1104 92-93 1.1215351774337101 1104 94-95 1.1987047289991182 1104 96-97 1.2221650307933771 1104 98-99 1.235783752333969 1104 100-101 1.5377515459343876 1105 1 0.12178661762052556 1105 2 0.2606241670110414 1105 3 0.22683592843691258 1105 4 0.05880516276534564 1105 5 0.06992711995741985 1105 6 0.11647352766342323 1105 7 0.16916461298747265 1105 8 0.16867558722385212 1105 9 0.3077733969883738 1105 10-11 0.2561028557559908 1105 12-13 0.1399358918874043 1105 14-15 0.3078659476456451 1105 16-17 0.3338021736439245 1105 18-19 0.3051825225289875 1105 20-21 0.2394497784330767 1105 22-23 0.3680613389492109 1105 24-25 0.45864831598090205 1105 26-27 0.3241393297222288 1105 28-29 0.42935499098431507 1105 30-31 0.46323115300699413 1105 32-33 0.5911867361655325 1105 34-35 0.6058353603848872 1105 36-37 0.5389685441710625 1105 38-39 0.35991447921065145 1105 40-41 0.25773552008698886 1105 42-43 0.2171047759978677 1105 44-45 0.28778779919972663 1105 46-47 0.32000328923552956 1105 48-49 0.45418279784670545 1105 50-51 0.5792773590490796 1105 52-53 0.3815275191959273 1105 54-55 0.4407450634111356 1105 56-57 0.6035678446734636 1105 58-59 0.5146505262202865 1105 60-61 0.3872328249362127 1105 62-63 0.36120621616454684 1105 64-65 0.5273163942142887 1105 66-67 0.46817262557833317 1105 68-69 0.45344854556269354 1105 70-71 0.2354966090675248 1105 72-73 0.3763256490057678 1105 74-75 0.43419897895183723 1105 76-77 0.5796198644919137 1105 78-79 0.506998153253658 1105 80-81 0.5408408281244199 1105 82-83 0.49456716123572875 1105 84-85 0.566029247791576 1105 86-87 0.6362803177201535 1105 88-89 0.7736658954011091 1105 90-91 0.5490127501615341 1105 92-93 0.683916029614231 1105 94-95 0.5920065717542293 1105 96-97 0.42736061340535514 1105 98-99 0.7299342927771093 1105 100-101 1.0181443074549321 1106 1 0.11382197882292999 1106 2 0.20483077881245393 1106 3 0.2415078869858398 1106 4 0.021571505081148246 1106 5 0.1058884327196381 1106 6 0.10651853825203972 1106 7 0.19199700905291905 1106 8 0.2119260678785011 1106 9 0.10674428631909905 1106 10-11 0.284772804536253 1106 12-13 0.20809800305530501 1106 14-15 0.2952362367735475 1106 16-17 0.19363063362342814 1106 18-19 0.28931381077129004 1106 20-21 0.2015471225286376 1106 22-23 0.3624774923666436 1106 24-25 0.23706461642074572 1106 26-27 0.2741644229346747 1106 28-29 0.266537953675531 1106 30-31 0.3153308804376067 1106 32-33 0.5648598993273524 1106 34-35 0.38602286482040427 1106 36-37 0.4501426175541994 1106 38-39 0.4440558001145902 1106 40-41 0.3560044005043679 1106 42-43 0.40417773772048804 1106 44-45 0.4245156396739276 1106 46-47 0.37378679318189967 1106 48-49 0.5073243501491191 1106 50-51 0.3564346066877633 1106 52-53 0.31942360994851526 1106 54-55 0.4846458177332167 1106 56-57 0.6606581971134702 1106 58-59 0.6923637543771122 1106 60-61 0.5613668894980037 1106 62-63 0.4004594671283428 1106 64-65 0.4657276413365494 1106 66-67 0.44506167289389253 1106 68-69 0.4706825315044867 1106 70-71 0.2742363218328876 1106 72-73 0.42863372754070284 1106 74-75 0.6503678152393739 1106 76-77 0.641484977899129 1106 78-79 0.7602324730010572 1106 80-81 0.5991444622364881 1106 82-83 0.741065612067942 1106 84-85 0.7626882558849744 1106 86-87 0.6909691884701132 1106 88-89 0.7242304175516772 1106 90-91 0.6642939270620936 1106 92-93 0.7926688102681148 1106 94-95 0.7514665972612313 1106 96-97 0.7995076330460265 1106 98-99 0.7899565403198601 1106 100-101 1.0576786578782347 1107 1 0.10928624651756991 1107 2 0.1251175465343195 1107 3 0.19985233675407343 1107 4 -0.05704482999814786 1107 5 0.06639491564298083 1107 6 0.09630593756889994 1107 7 0.18167959626929076 1107 8 0.1262269757055563 1107 9 0.17076496924025975 1107 10-11 0.20221058879936749 1107 12-13 0.0908252865071475 1107 14-15 0.13914549671844156 1107 16-17 0.12010891528576195 1107 18-19 0.20322326128364665 1107 20-21 0.1703879823690002 1107 22-23 0.11458711947150135 1107 24-25 0.19041347646437856 1107 26-27 0.22388841773671686 1107 28-29 0.2685386850915634 1107 30-31 0.19623359393668238 1107 32-33 0.08101468819676683 1107 34-35 0.019305516285236024 1107 36-37 0.21376592195758093 1107 38-39 0.1703351429653992 1107 40-41 0.1685306035294829 1107 42-43 0.21994313375596164 1107 44-45 0.16694693556893014 1107 46-47 0.1706262319335181 1107 48-49 0.17682230472509985 1107 50-51 0.29222029361991275 1107 52-53 0.18347868575806814 1107 54-55 0.3010235251522815 1107 56-57 0.23082762973063353 1107 58-59 0.12995302615534143 1107 60-61 0.1255589696960513 1107 62-63 0.06692864692601574 1107 64-65 0.03835891055209828 1107 66-67 0.03791736704945947 1107 68-69 0.2078926194196029 1107 70-71 0.04293847578961163 1107 72-73 0.025669654950835508 1107 74-75 0.14742105252670612 1107 76-77 -0.0018138082769247887 1107 78-79 0.03514202151952972 1107 80-81 0.28520783317512866 1107 82-83 0.2947306552813771 1107 84-85 0.17527337601507043 1107 86-87 0.25171154399275864 1107 88-89 0.27439140167831155 1107 90-91 0.26604639899423077 1107 92-93 0.06462127367008819 1107 94-95 0.12707266982932097 1107 96-97 0.29133943548746544 1107 98-99 0.34473209269323846 1107 100-101 0.7865125120959462 1108 1 0.2212307438205663 1108 2 0.29808225857313886 1108 3 0.3380514723948025 1108 4 0.1502348901280257 1108 5 0.14518215957379965 1108 6 0.1984994047400832 1108 7 0.16723121996195545 1108 8 0.23270198540306808 1108 9 0.2626260130583731 1108 10-11 0.19561673885097264 1108 12-13 0.3511837833563831 1108 14-15 0.44769307490233956 1108 16-17 0.3380474348966871 1108 18-19 0.3971018905313386 1108 20-21 0.4576584441168947 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-1.1280937086312228 2316 90-91 -0.5923143719142523 2316 92-93 -0.772833637830626 2316 94-95 -0.546217637191905 2316 96-97 -0.4915138276615991 2316 98-99 -0.3789912736350871 2316 100-101 -0.47078133909103315 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5.0 3 10.0 4 28.0 5 76.0 6 142.0 7 379.0 8 886.0 9 1634.0 10 2676.0 11 4109.0 12 5704.0 13 7263.0 14 8256.0 15 10391.0 16 12159.0 17 13374.0 18 12622.0 19 11133.0 20 11086.0 21 11846.0 22 13278.0 23 15477.0 24 19013.0 25 23628.0 26 30082.0 27 38027.0 28 48167.0 29 59262.0 30 72680.0 31 86857.0 32 103021.0 33 124640.0 34 152496.0 35 188968.0 36 242260.0 37 314291.0 38 274450.0 39 47549.0 40 12.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.26040427820098 21.57138094163521 14.742452057273084 23.425762722890735 2 15.35835119524929 22.130990093877962 39.782628359336016 22.728030351536734 3 18.11909635480717 26.686864662586412 31.0907019430323 24.103337039574114 4 12.731195192410825 18.297315555989332 39.13511584604287 29.83637340555697 5 12.6264294454585 38.38348164740413 36.04804318927423 12.94204571786314 6 29.89243552635858 39.188469543725 17.875632134378353 13.043462795538066 7 25.75814164782714 33.12245259883827 23.57224850185753 17.547157251477056 8 24.466128742942484 36.93690397609274 21.059922141816532 17.537045139148255 9 25.1267189955776 16.22582430230236 21.222681417138862 37.42477528498117 10-11 23.71821354037248 27.095862316730667 29.65450621640835 19.5314179264885 12-13 24.363279922070678 24.550785924549416 29.7156108147771 21.370323338602812 14-15 21.631307303028503 26.34540638241976 26.99362327147668 25.029663043075058 16-17 21.357797531120156 29.28335612369705 27.749084447317163 21.609761897865635 18-19 21.269100586045184 28.302735300977623 29.60160818156272 20.826555931414468 20-21 21.85893145969612 27.534290985941112 29.598559303473635 21.00821825088913 22-23 21.598060099515433 27.370453104165986 29.536351791928194 21.495135004390388 24-25 21.541881380762597 27.571576587952702 29.541492138681974 21.345049892602724 26-27 21.636610137796424 27.625878917812514 29.372281052470573 21.365229891920485 28-29 21.55298749552831 27.809898289375262 29.30057176656151 21.336542448534914 30-31 21.77432837944624 27.53785289775716 29.345162019803688 21.34265670299291 32-33 21.613420905171594 27.69999402803773 29.168995096637783 21.517589970152894 34-35 21.761199984245504 27.759850688052122 29.25324968205144 21.225699645650934 36-37 21.80674292961938 27.634014049294485 29.167276445073192 21.391966576012944 38-39 21.72693485425773 27.678835484667612 29.177494478959066 21.416735182115588 40-41 21.800073529841455 27.608905596982787 29.196250740316433 21.39477013285933 42-43 21.707851476018742 27.666810737906466 29.03797763617972 21.587360149895073 44-45 21.692302141346016 27.810665922681437 28.902199990751004 21.594831945221546 46-47 21.84986945998996 27.767327518850486 28.664045279279392 21.718757741880157 48-49 21.75223630596101 27.84011619338596 28.655741520535592 21.751905980117435 50-51 21.82127924761162 27.955996969592057 28.48339079839437 21.73933298440195 52-53 21.873331780989343 27.968069843277654 28.463642104042975 21.694956271690025 54-55 21.740566746145877 27.851360070298824 28.601715642987486 21.806357540567813 56-57 21.787885379049445 28.021489649536672 28.362515741424925 21.82810922998895 58-59 21.70293025148134 27.979100017966402 28.526478079592433 21.79149165095983 60-61 21.78646440539569 28.04605789318746 28.418815260503074 21.748662440913773 62-63 21.705274150479788 28.024483212299998 28.423940259985347 21.846302377234867 64-65 21.567497863508727 27.95212224799577 28.407093151019414 22.073286737476092 66-67 21.538020915204246 27.94461850889255 28.39411684209616 22.123243733807048 68-69 21.611153375703058 27.95308045287634 28.399643807414883 22.036122364005728 70-71 21.56422873233247 27.697153053148572 28.445587974991305 22.29303023952766 72-73 21.600429181275697 27.841660585751082 28.305598425657312 22.252311807315913 74-75 21.574258331064375 28.010057615814254 28.165781539761685 22.249902513359682 76-77 21.492750000191123 27.90920560510697 28.204067726294763 22.393976668407145 78-79 21.39940542738083 27.907841244028862 28.23119727614595 22.461556052444354 80-81 21.45515126076895 27.969903339861357 28.068382652599745 22.50656274676995 82-83 21.504524789157376 27.857776951193053 28.16154683813557 22.476151421514 84-85 21.472918086300528 27.887503512561633 28.04497806586288 22.59460033527496 86-87 21.349489338327395 27.913698456810355 28.109995391112623 22.626816813749627 88-89 21.30572877007402 27.770219095087214 28.08394888958505 22.84010324525372 90-91 21.326749050661455 27.760882297265884 28.100166809168027 22.81220184290464 92-93 21.29555983035213 27.66675730138925 28.126249933294538 22.91143293496408 94-95 21.113337102132334 27.540045606702535 28.239771839449162 23.10684545171597 96-97 21.08253465431058 27.43672688709039 28.24043655869065 23.24030189990838 98-99 20.983090413971585 27.51820815402119 28.116372627782294 23.382328804224933 100-101 20.990458536020206 27.461753094738295 28.104278744695588 23.443509624545907 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 335.0 1 255.0 2 220.0 3 282.0 4 259.0 5 260.5 6 365.5 7 637.5 8 1048.0 9 1240.0 10 1123.5 11 1017.0 12 1073.0 13 1184.0 14 1365.0 15 1529.0 16 1618.5 17 1794.5 18 2050.0 19 2409.5 20 2864.0 21 3396.0 22 4195.0 23 5341.5 24 6844.5 25 8821.0 26 11566.0 27 14987.0 28 18958.5 29 23761.5 30 28946.5 31 34440.0 32 40313.5 33 44548.5 34 48321.0 35 52027.0 36 56551.5 37 61847.5 38 65183.5 39 67220.5 40 68790.5 41 70182.0 42 71638.0 43 73495.0 44 75046.5 45 76328.0 46 76685.0 47 76370.0 48 75563.0 49 73638.0 50 70198.5 51 66541.0 52 63759.0 53 60348.5 54 56011.0 55 50782.5 56 44670.0 57 38309.5 58 33041.5 59 28267.5 60 23183.0 61 18571.0 62 14722.0 63 11095.0 64 7925.0 65 5807.0 66 4314.5 67 3334.5 68 2521.5 69 1793.0 70 1341.5 71 1005.0 72 744.0 73 572.0 74 428.5 75 308.5 76 213.0 77 138.5 78 85.0 79 57.0 80 39.0 81 15.5 82 9.0 83 7.5 84 4.5 85 1.5 86 1.0 87 1.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.019360375865690824 2 0.003201321993539427 3 0.004319243959537322 4 0.036281649260113506 5 0.002083400027541532 6 5.081463481808615E-5 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 5.081463481808615E-5 24-25 5.081463481808614E-4 26-27 0.0017276975838149292 28-29 5.081463481808615E-5 30-31 0.00619938544780651 32-33 0.020808592958006276 34-35 0.012424178213022063 36-37 4.573317133627753E-4 38-39 5.589609829989477E-4 40-41 0.0014482170923154553 42-43 0.008155748888302826 44-45 0.00792708303162144 46-47 0.0075967879053038785 48-49 0.009299078171709765 50-51 0.19538227087554125 52-53 0.05178011287962979 54-55 0.01709912461628599 56-57 0.010112112328799144 58-59 0.01908089537419135 60-61 0.056302615378439454 62-63 0.10256934038030689 64-65 0.10655828921352666 66-67 0.11128405025160866 68-69 0.10907361363702191 70-71 0.09659862078918176 72-73 0.07124211801495678 74-75 0.24632394228067261 76-77 0.29642717221130555 78-79 0.006961604970077802 80-81 5.589609829989477E-4 82-83 0.0 84-85 0.0 86-87 0.0 88-89 7.622195222712922E-5 90-91 0.006072348860761295 92-93 0.0168704587596046 94-95 0.0012449585530431107 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1967937.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 69.53106316411073 #Duplication Level Percentage of deduplicated Percentage of total 1 82.89432746381064 57.63730718832696 2 10.433220781381003 14.508658663106305 3 2.9624467988874237 6.1794622648127735 4 1.2707030262573193 3.534133295260973 5 0.6895462773895149 2.3972442883873892 6 0.42344084263660176 1.7665375185377898 7 0.28148826405328464 1.370052478749137 8 0.1978948988088986 1.1007874167149465 9 0.1461940517105557 0.9148525059333527 >10 0.6695967322897326 7.832799539035326 >50 0.02220474238090407 1.048066066293245 >100 0.008423757567324401 1.0597874353105798 >500 2.1958406864827147E-4 0.09735839666013467 >1k 2.9277875819769256E-4 0.5529529428710689 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4057 0.2061549734569755 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3363 0.1708896168932237 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2229 0.11326582100951402 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 2.5407317409043076E-5 16-17 0.0 0.0 0.0 0.0 5.081463481808615E-5 18-19 0.0 0.0 0.0 0.0 5.081463481808615E-5 20-21 2.5407317409043076E-5 0.0 0.0 1.2703658704521538E-4 5.081463481808615E-5 22-23 5.081463481808615E-5 0.0 0.0 3.3029512631755996E-4 5.081463481808615E-5 24-25 5.081463481808615E-5 0.0 0.0 5.589609829989476E-4 1.016292696361723E-4 26-27 5.081463481808615E-5 0.0 0.0 0.0012449585530431104 1.5244390445425844E-4 28-29 5.081463481808615E-5 0.0 0.0 0.0034553951676298583 1.5244390445425844E-4 30-31 5.081463481808615E-5 0.0 0.0 0.011916031864841201 1.5244390445425844E-4 32-33 5.081463481808615E-5 0.0 0.0 0.03302951263175599 1.5244390445425844E-4 34-35 5.081463481808615E-5 0.0 0.0 0.06405184718819759 1.5244390445425844E-4 36-37 5.081463481808615E-5 0.0 0.0 0.11357070881842254 1.5244390445425844E-4 38-39 5.081463481808615E-5 0.0 0.0 0.18206883655320266 2.2866585668138766E-4 40-41 5.081463481808615E-5 0.0 0.0 0.25079563014466416 2.540731740904307E-4 42-43 5.081463481808615E-5 0.0 0.0 0.326153733579886 2.540731740904307E-4 44-45 5.081463481808615E-5 0.0 0.0 0.4190174787099384 2.540731740904307E-4 46-47 5.081463481808615E-5 0.0 0.0 0.5279894630773241 2.794804914994738E-4 48-49 5.081463481808615E-5 0.0 0.0 0.6424494280050632 3.048878089085169E-4 50-51 5.081463481808615E-5 0.0 0.0 0.7635661100939715 3.048878089085169E-4 52-53 5.081463481808615E-5 0.0 0.0 0.880134882366661 3.048878089085169E-4 54-55 5.081463481808615E-5 0.0 0.0 1.0096105718831445 3.048878089085169E-4 56-57 5.081463481808615E-5 0.0 0.0 1.1595645592313168 3.048878089085169E-4 58-59 5.081463481808615E-5 0.0 0.0 1.3196814735431064 3.048878089085169E-4 60-61 5.081463481808615E-5 0.0 0.0 1.4961861075837286 3.048878089085169E-4 62-63 5.081463481808615E-5 0.0 0.0 1.676400209966071 4.573317133627753E-4 64-65 5.081463481808615E-5 0.0 0.0 1.8604254099597701 4.573317133627753E-4 66-67 5.081463481808615E-5 0.0 0.0 2.064573205341431 4.573317133627753E-4 68-69 7.622195222712922E-5 0.0 0.0 2.2887927814762365 4.573317133627753E-4 70-71 1.016292696361723E-4 0.0 0.0 2.538749970146402 4.573317133627753E-4 72-73 1.016292696361723E-4 0.0 0.0 2.8020205931389066 4.573317133627753E-4 74-75 1.016292696361723E-4 0.0 0.0 3.069432608869085 4.573317133627753E-4 76-77 1.016292696361723E-4 0.0 0.0 3.3589235834277216 4.573317133627753E-4 78-79 1.016292696361723E-4 0.0 0.0 3.680656443778434 4.573317133627753E-4 80-81 1.016292696361723E-4 0.0 0.0 4.03122660938841 4.827390307718184E-4 82-83 1.016292696361723E-4 0.0 0.0 4.391578592200869 5.081463481808614E-4 84-85 1.016292696361723E-4 0.0 0.0 4.763846606878167 5.081463481808614E-4 86-87 1.016292696361723E-4 0.0 0.0 5.1549668510729765 5.335536655899046E-4 88-89 1.2703658704521536E-4 0.0 0.0 5.58368992503317 5.589609829989476E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2270 0.0 52.093037 1 GTATCAA 4055 0.0 41.693207 1 ATCAACG 5190 0.0 31.649025 3 TCAACGC 5240 0.0 31.615612 4 CAACGCA 5310 0.0 31.100748 5 TATCAAC 5525 0.0 29.989336 2 AACGCAG 5545 0.0 29.782679 6 ATAACGC 220 1.8189894E-12 28.052546 3 TAACGCA 180 6.7520887E-9 26.371508 4 ACGCAGA 6615 0.0 24.821745 7 CGCAGAG 6745 0.0 24.272987 8 GTGGTAT 1175 0.0 22.633741 1 GTACATG 4500 0.0 22.056673 1 TATATCG 175 4.0847644E-6 21.693918 5 TACATGG 4610 0.0 20.905895 2 GCAGAGT 7875 0.0 20.548964 9 AGAGTAC 7255 0.0 20.244604 10-11 ACATGGG 4680 0.0 19.882082 3 TATAACG 265 1.8675564E-8 19.707 2 GAGTACT 5440 0.0 18.3192 12-13 >>END_MODULE