FastQCFastQC Report
Sat 18 Jun 2016
SRR1930903_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1930903_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1224886
Sequences flagged as poor quality0
Sequence length101
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN22050.18001675257942373No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA11250.055.0220831
GTATCAA18750.041.3336641
TCAACGC24950.031.2401944
TATCAAC25200.030.9437772
ATCAACG25400.030.6934243
CAACGCA25400.029.2976555
GTACATG32050.027.9496541
GTATTAA2650.026.5868361
AACGCAG28300.026.3989666
TACATGG33450.026.1694932
GTGGTAT5000.025.1625391
ACATGGG36250.024.0040993
CTAGTAC2904.9112714E-1122.5472053
TAGTACT3350.022.5164034
ACGCAGA32600.022.5112867
GTATAAG3155.456968E-1222.3667011
CATGGGG30400.021.8349824
CGCAGAG35250.021.807398
TATAAGG3007.6397555E-1121.8003922
CCTAGTA3059.640644E-1121.4430072
Produced by FastQC (version 0.11.5)