##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1930903_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1224886 Sequences flagged as poor quality 0 Sequence length 101 %GC 51 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 22.362102269109126 30.0 10.0 31.0 2.0 33.0 2 21.305657832647285 28.0 10.0 31.0 2.0 33.0 3 21.734659388710458 28.0 10.0 31.0 2.0 33.0 4 23.643814199852066 32.0 2.0 35.0 2.0 37.0 5 23.599699074036277 32.0 10.0 35.0 2.0 37.0 6 24.297853841092152 32.0 10.0 35.0 2.0 37.0 7 24.31788101096755 32.0 10.0 35.0 2.0 37.0 8 25.008653050161403 32.0 16.0 35.0 2.0 37.0 9 26.0754911069275 33.0 17.0 37.0 2.0 39.0 10-11 26.243974949505507 32.5 16.5 37.0 2.0 39.0 12-13 26.1051661134179 33.0 15.5 37.0 2.0 39.0 14-15 26.294203705487696 33.0 10.5 38.0 2.0 40.0 16-17 25.409620568771295 32.0 10.0 38.0 2.0 40.0 18-19 24.765887600968583 32.0 9.0 38.0 2.0 40.0 20-21 24.197075074741647 31.0 2.0 37.5 2.0 40.0 22-23 24.065858781960117 31.0 8.0 37.0 2.0 39.0 24-25 23.70812753186827 30.0 2.0 37.0 2.0 39.0 26-27 23.301775838731118 30.0 2.0 37.0 2.0 39.0 28-29 23.144995534278294 30.0 2.0 37.0 2.0 39.0 30-31 22.98790009845814 30.0 2.0 37.0 2.0 39.0 32-33 22.5914697367755 30.0 2.0 37.0 2.0 39.0 34-35 19.844541043003183 23.5 2.0 35.0 2.0 38.0 36-37 18.225794482098742 16.5 2.0 34.0 2.0 38.0 38-39 17.272583734323028 15.0 2.0 33.0 2.0 38.0 40-41 18.341278698589093 16.0 2.0 33.5 2.0 38.0 42-43 18.186950867264382 16.5 2.0 33.5 2.0 38.0 44-45 16.349330060103554 11.5 2.0 32.0 2.0 37.5 46-47 16.961754400001304 14.5 2.0 31.5 2.0 37.0 48-49 16.39162624113591 9.0 2.0 32.0 2.0 37.0 50-51 14.59178854195411 7.0 2.0 30.0 2.0 36.5 52-53 17.126268893594997 16.0 2.0 32.0 2.0 37.0 54-55 18.49735934609425 21.5 2.0 34.0 2.0 38.0 56-57 18.59413569915894 22.0 2.0 34.0 2.0 38.0 58-59 18.20910925588177 20.5 2.0 34.0 2.0 38.0 60-61 17.812801354575036 18.5 2.0 34.0 2.0 37.5 62-63 17.29078828560372 15.0 2.0 33.0 2.0 37.0 64-65 16.752048353887627 10.5 2.0 32.5 2.0 36.0 66-67 16.29000127358791 7.5 2.0 32.0 2.0 36.0 68-69 15.789305698652772 4.5 2.0 31.5 2.0 35.0 70-71 15.207995682863547 2.0 2.0 31.0 2.0 35.0 72-73 14.709777889534209 2.0 2.0 30.5 2.0 34.0 74-75 14.177571627073867 2.0 2.0 30.0 2.0 34.0 76-77 13.621128823417036 2.0 2.0 29.5 2.0 34.0 78-79 13.131600410160619 2.0 2.0 29.0 2.0 34.0 80-81 12.638753728918445 2.0 2.0 29.0 2.0 34.0 82-83 12.166577950927678 2.0 2.0 27.5 2.0 33.5 84-85 11.677977787320616 2.0 2.0 26.5 2.0 33.0 86-87 11.149459623181258 2.0 2.0 25.5 2.0 33.0 88-89 10.630550516537866 2.0 2.0 25.0 2.0 32.0 90-91 10.098298943738438 2.0 2.0 23.5 2.0 32.0 92-93 9.52036965072668 2.0 2.0 19.5 2.0 32.0 94-95 8.92128777698496 2.0 2.0 14.0 2.0 31.5 96-97 8.269227503620744 2.0 2.0 2.0 2.0 31.0 98-99 7.578632215569449 2.0 2.0 2.0 2.0 31.0 100-101 6.732402443982542 2.0 2.0 2.0 2.0 29.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 6.771043724240229 1101 2 7.785712851016797 1101 3 7.604748376133646 1101 4 9.432155694057489 1101 5 9.315052465005085 1101 6 8.627404402188901 1101 7 8.640068163671174 1101 8 7.636048794081031 1101 9 7.751329683303023 1101 10-11 7.404748536379685 1101 12-13 7.115643907919054 1101 14-15 7.585057604834784 1101 16-17 8.254784325523655 1101 18-19 8.783045414788475 1101 20-21 9.141437371367136 1101 22-23 8.731580933858421 1101 24-25 8.886943813079984 1101 26-27 9.182058479842194 1101 28-29 8.85041682451747 1101 30-31 8.830286995192282 1101 32-33 9.04175273838646 1101 34-35 11.032204688975956 1101 36-37 5.225814875221719 1101 38-39 8.094651607420719 1101 40-41 9.431749759248682 1101 42-43 10.170531886758265 1101 44-45 4.843420326825363 1101 46-47 6.2089481805136515 1101 48-49 9.275774980837454 1101 50-51 -2.462670202814044 1101 52-53 5.987514477067933 1101 54-55 8.278529019642281 1101 56-57 8.88439522789923 1101 58-59 9.003914164681891 1101 60-61 9.083044740337503 1101 62-63 9.121762592386428 1101 64-65 8.766515320451191 1101 66-67 8.775733561710961 1101 68-69 8.80452903094258 1101 70-71 8.759247105294365 1101 72-73 8.738039504355498 1101 74-75 8.593577182093115 1101 76-77 8.412136168581247 1101 78-79 8.578589473743644 1101 80-81 8.40470919194682 1101 82-83 8.393093153093949 1101 84-85 8.15435416269048 1101 86-87 7.820802446195822 1101 88-89 7.7214556998607105 1101 90-91 7.315045093306159 1101 92-93 7.235640149029727 1101 94-95 6.745181642686449 1101 96-97 6.66400846194097 1101 98-99 6.244715113120804 1101 100-101 5.914996467899198 1102 1 6.991060245288679 1102 2 7.7714888637252955 1102 3 7.60674297502165 1102 4 9.189702953787435 1102 5 9.35130155158173 1102 6 8.601219175818763 1102 7 8.408568878524228 1102 8 7.656879294478177 1102 9 7.744366696805802 1102 10-11 7.430627487928533 1102 12-13 7.105029134289186 1102 14-15 7.495900813730724 1102 16-17 8.170169234181326 1102 18-19 8.290163762683633 1102 20-21 8.883445949603836 1102 22-23 8.78517775673372 1102 24-25 8.599782574001345 1102 26-27 8.785342355934333 1102 28-29 8.560489262960683 1102 30-31 8.650272698131118 1102 32-33 8.46923740875977 1102 34-35 10.93468824735562 1102 36-37 1.831787551949283 1102 38-39 4.175309589946533 1102 40-41 8.848847138120803 1102 42-43 10.012246898672483 1102 44-45 2.024923106809478 1102 46-47 2.6553850351601973 1102 48-49 8.01469475843873 1102 50-51 -3.615150584069008 1102 52-53 5.446913682786757 1102 54-55 8.22679176785515 1102 56-57 8.97275328985316 1102 58-59 9.095142123379272 1102 60-61 8.983242357493978 1102 62-63 9.084506039566733 1102 64-65 8.880887997178753 1102 66-67 8.721415213815806 1102 68-69 8.635987966605809 1102 70-71 8.442102704976653 1102 72-73 8.276812181083063 1102 74-75 8.265034529193988 1102 76-77 8.09824927422064 1102 78-79 8.311163580177322 1102 80-81 8.104490923479785 1102 82-83 7.613592279384653 1102 84-85 7.429540501054261 1102 86-87 7.003063923558807 1102 88-89 7.212542276508847 1102 90-91 7.147837785919345 1102 92-93 6.836551983819245 1102 94-95 6.55038450209868 1102 96-97 6.289730145817062 1102 98-99 6.018861896282043 1102 100-101 5.08563665852668 1103 1 6.5930445166842055 1103 2 7.4630570981123405 1103 3 7.337915218660818 1103 4 8.903800101050354 1103 5 8.936929307942567 1103 6 8.116036138039277 1103 7 7.883897174592079 1103 8 7.253760713136618 1103 9 7.530713034508192 1103 10-11 7.004187395407328 1103 12-13 6.8047299824002145 1103 14-15 7.15611453770914 1103 16-17 7.828691362168993 1103 18-19 8.198223901465433 1103 20-21 8.17378488561 1103 22-23 8.07786303044228 1103 24-25 8.375949112166342 1103 26-27 8.560411592634406 1103 28-29 8.310929047077874 1103 30-31 7.909373700444149 1103 32-33 7.9017697140797445 1103 34-35 9.919511377656317 1103 36-37 -2.5188267888371474 1103 38-39 -1.625921867262413 1103 40-41 6.418203498953492 1103 42-43 8.498728317330926 1103 44-45 0.8010248801325304 1103 46-47 0.659153732153257 1103 48-49 6.6924896697725735 1103 50-51 -4.292635240969547 1103 52-53 4.261054931822997 1103 54-55 7.055494620592235 1103 56-57 7.595565626013531 1103 58-59 7.840903110272098 1103 60-61 7.78454505602906 1103 62-63 7.796840657916768 1103 64-65 7.880778822159478 1103 66-67 7.556777125920664 1103 68-69 7.2648820298286285 1103 70-71 7.296072172979736 1103 72-73 7.000722435516369 1103 74-75 7.116045785940328 1103 76-77 6.898525912616941 1103 78-79 6.979277381256736 1103 80-81 6.641012434659428 1103 82-83 6.253393975606704 1103 84-85 6.173899174917024 1103 86-87 6.01985837228664 1103 88-89 5.725515291158034 1103 90-91 5.7399441853025355 1103 92-93 5.208114050126106 1103 94-95 4.840389744966837 1103 96-97 4.666940014745357 1103 98-99 4.222272536220362 1103 100-101 3.679632032466542 1104 1 6.248321200557822 1104 2 7.380001373202173 1104 3 6.765257607318844 1104 4 8.474439875697975 1104 5 8.345068344606752 1104 6 7.78121553669822 1104 7 7.441049242436282 1104 8 6.349275352097582 1104 9 6.724307864356977 1104 10-11 6.314914371931565 1104 12-13 5.71050601071072 1104 14-15 6.3043954308649255 1104 16-17 7.012145276637735 1104 18-19 7.361777106125629 1104 20-21 7.635697048921497 1104 22-23 7.375523169925533 1104 24-25 7.4741389045547955 1104 26-27 7.276676396874439 1104 28-29 7.2411646230819855 1104 30-31 6.970256018828621 1104 32-33 6.9560522685020025 1104 34-35 9.150579983519382 1104 36-37 -3.200345579210687 1104 38-39 -2.2804935942839464 1104 40-41 4.7297193140116285 1104 42-43 7.143522713683854 1104 44-45 0.2159071856570911 1104 46-47 -0.5230258822014413 1104 48-49 5.801048056391721 1104 50-51 -4.7537974377460355 1104 52-53 3.2713203545077576 1104 54-55 5.898425732980247 1104 56-57 6.538564662104864 1104 58-59 6.563378666214749 1104 60-61 6.394280416629687 1104 62-63 6.564374121446946 1104 64-65 6.824054638573749 1104 66-67 6.557123174392 1104 68-69 6.529832697462515 1104 70-71 6.460311044627902 1104 72-73 5.900961688285488 1104 74-75 6.107872436699674 1104 76-77 6.031462011142549 1104 78-79 5.876074270093923 1104 80-81 5.54937856563293 1104 82-83 5.357212901067745 1104 84-85 5.078120031001189 1104 86-87 4.821930337508769 1104 88-89 4.6395339368575925 1104 90-91 4.452442789710092 1104 92-93 4.099730149095969 1104 94-95 3.9013425005823787 1104 96-97 3.6183824581749082 1104 98-99 3.0663806225898513 1104 100-101 2.806958872325011 1105 1 5.889213324010424 1105 2 7.1120936961493015 1105 3 6.722974931373951 1105 4 8.394360786990866 1105 5 8.465353617053285 1105 6 7.390421358203799 1105 7 7.302576049217915 1105 8 6.446569707572483 1105 9 6.282540352300739 1105 10-11 6.200074720976076 1105 12-13 5.861980030625897 1105 14-15 6.184841421680847 1105 16-17 6.679869000354358 1105 18-19 7.293909670711688 1105 20-21 7.5985468069693844 1105 22-23 7.467234810513926 1105 24-25 7.30799145942613 1105 26-27 7.427748591320146 1105 28-29 7.209153019780633 1105 30-31 6.892677998209063 1105 32-33 6.978139669087131 1105 34-35 8.72476494260114 1105 36-37 -3.2658002890483786 1105 38-39 -2.4170604802015596 1105 40-41 4.900969351682765 1105 42-43 6.945629283707557 1105 44-45 -3.0148617723799216 1105 46-47 -3.9339119250892196 1105 48-49 4.071125000059929 1105 50-51 -8.669917848293482 1105 52-53 0.37904680827389825 1105 54-55 4.7445909884319235 1105 56-57 5.748494833111149 1105 58-59 6.323924664298989 1105 60-61 6.243612115872779 1105 62-63 6.030976811195728 1105 64-65 5.912468355713436 1105 66-67 5.925430490511051 1105 68-69 5.535604802147505 1105 70-71 5.832498340206582 1105 72-73 5.959986304231933 1105 74-75 5.568356742755951 1105 76-77 5.396123942965771 1105 78-79 5.37847488181411 1105 80-81 5.323047119894438 1105 82-83 4.920505139867897 1105 84-85 4.598530992090895 1105 86-87 4.4098866827170315 1105 88-89 4.503901590616405 1105 90-91 4.579808167836724 1105 92-93 4.013343877817686 1105 94-95 3.54603157926158 1105 96-97 3.323733887048549 1105 98-99 3.2240687552064395 1105 100-101 2.622088723998231 1106 1 5.845613669946214 1106 2 6.918800888078415 1106 3 6.524067784762899 1106 4 8.137349833543905 1106 5 7.857321642905934 1106 6 6.976570165164276 1106 7 7.096399624338538 1106 8 6.122680207720183 1106 9 6.458898203655121 1106 10-11 5.851926118065521 1106 12-13 5.524088495019786 1106 14-15 5.929893507794656 1106 16-17 6.240137575125008 1106 18-19 6.5154666546166595 1106 20-21 6.862087654322252 1106 22-23 6.590469689762639 1106 24-25 6.486583182829353 1106 26-27 6.698186800378775 1106 28-29 6.7737653664614434 1106 30-31 6.636306183671454 1106 32-33 6.5018346690337445 1106 34-35 8.38665719712731 1106 36-37 -0.15242979964888903 1106 38-39 0.9986989354564244 1106 40-41 6.037707412093404 1106 42-43 7.021757400955586 1106 44-45 -3.1050251428100033 1106 46-47 -4.2771446452051 1106 48-49 3.8215440735072193 1106 50-51 -8.699984069609343 1106 52-53 0.07438278476544724 1106 54-55 4.189144279270675 1106 56-57 5.053081448148443 1106 58-59 5.4642623669104715 1106 60-61 5.604684671040403 1106 62-63 5.488445156766122 1106 64-65 5.300751619705391 1106 66-67 4.967969856129354 1106 68-69 4.812705774824982 1106 70-71 4.840185505585483 1106 72-73 4.567176869058706 1106 74-75 4.415520982770852 1106 76-77 4.388055666914708 1106 78-79 4.411713960694826 1106 80-81 4.043091227893788 1106 82-83 4.043709174361824 1106 84-85 3.866677791769634 1106 86-87 3.618961031625778 1106 88-89 3.358974544393165 1106 90-91 3.1452076489330416 1106 92-93 2.851386078187584 1106 94-95 2.6587875462387114 1106 96-97 2.459132276715083 1106 98-99 2.057281378778236 1106 100-101 1.8945742536560557 1107 1 5.8880876200082 1107 2 7.108395370830756 1107 3 6.570885383398615 1107 4 7.714058974729845 1107 5 8.014355553077621 1107 6 6.473582377015475 1107 7 6.70980807074562 1107 8 6.041186848704054 1107 9 6.023405739438562 1107 10-11 5.737442147614399 1107 12-13 5.599308865029652 1107 14-15 5.937113379175756 1107 16-17 6.904370580479007 1107 18-19 6.863113650492156 1107 20-21 7.544883197173018 1107 22-23 7.318617397721006 1107 24-25 7.2728177273296915 1107 26-27 7.213166439703965 1107 28-29 7.267930100657015 1107 30-31 6.888368434855131 1107 32-33 6.638049974204801 1107 34-35 8.52915915236684 1107 36-37 -0.16912703294226716 1107 38-39 0.7928853565882683 1107 40-41 6.17803128172589 1107 42-43 7.305227003384527 1107 44-45 -2.920937775763445 1107 46-47 -3.984070462222002 1107 48-49 3.876774040413295 1107 50-51 -8.581814458414557 1107 52-53 0.12763559752123754 1107 54-55 4.554884213629268 1107 56-57 5.651376775268272 1107 58-59 6.022884830933496 1107 60-61 5.895330315609161 1107 62-63 5.610877019372264 1107 64-65 5.682343793738216 1107 66-67 5.673227031317676 1107 68-69 5.624295820008948 1107 70-71 5.668420132052196 1107 72-73 5.68976924914589 1107 74-75 5.986779929343575 1107 76-77 5.565919281700074 1107 78-79 5.111833438068121 1107 80-81 4.938639315401998 1107 82-83 4.945019953281436 1107 84-85 4.7713933058411 1107 86-87 4.771688007328441 1107 88-89 4.880564714803533 1107 90-91 4.459208017781721 1107 92-93 4.228127152106454 1107 94-95 3.8954950480971835 1107 96-97 3.7567647007908835 1107 98-99 3.420414327097302 1107 100-101 2.883506516417479 1108 1 5.960157643058196 1108 2 6.286091094208565 1108 3 6.315742231527228 1108 4 7.665420425906397 1108 5 8.081035380578015 1108 6 7.520086090316905 1108 7 7.217199361795604 1108 8 6.524501599310888 1108 9 5.098502012790934 1108 10-11 5.820569495363479 1108 12-13 5.388761476296626 1108 14-15 6.41495062785712 1108 16-17 6.044839866151587 1108 18-19 6.686290156735673 1108 20-21 7.414595391983017 1108 22-23 7.541481102458629 1108 24-25 7.185195213406551 1108 26-27 7.526418935859983 1108 28-29 7.354358407942097 1108 30-31 7.073536638651568 1108 32-33 7.2534336912876505 1108 34-35 9.143317243638148 1108 36-37 -3.1933559424745095 1108 38-39 -2.377940967674288 1108 40-41 5.054387658566899 1108 42-43 7.383967344769136 1108 44-45 -2.694749755644052 1108 46-47 -3.7952618049885025 1108 48-49 4.623244944312333 1108 50-51 -8.637958019013245 1108 52-53 0.46443189840013943 1108 54-55 5.0674802436915805 1108 56-57 6.130988234091081 1108 58-59 6.383261082487078 1108 60-61 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4.615302638946236 1109 22-23 6.0279405685251035 1109 24-25 6.189950374454863 1109 26-27 5.028307737642571 1109 28-29 4.663107252378673 1109 30-31 4.507253801683653 1109 32-33 4.127309668774888 1109 34-35 6.097318935186312 1109 36-37 -4.063285161580573 1109 38-39 -3.2030840608471216 1109 40-41 2.8832664880830094 1109 42-43 3.555918554001277 1109 44-45 -4.270816878073363 1109 46-47 -5.615520482602463 1109 48-49 1.4545284697010104 1109 50-51 -8.913335315739452 1109 52-53 -1.08399274200538 1109 54-55 2.2238956227304314 1109 56-57 3.957113380556109 1109 58-59 4.575616802139663 1109 60-61 4.606240392263441 1109 62-63 4.653048745538008 1109 64-65 4.16486804253023 1109 66-67 4.409895637096231 1109 68-69 4.454506787467485 1109 70-71 4.63776831344226 1109 72-73 4.512053602095911 1109 74-75 4.525710568498601 1109 76-77 4.6398132953846645 1109 78-79 4.491671819028419 1109 80-81 4.51854655462113 1109 82-83 4.131782150888812 1109 84-85 3.9011495713490465 1109 86-87 3.9776078389027223 1109 88-89 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-11.86533426061493 2313 76-77 -11.342337986925855 2313 78-79 -10.85500457268892 2313 80-81 -10.334614809004291 2313 82-83 -9.882351669659933 2313 84-85 -9.37922255627058 2313 86-87 -8.866080025485779 2313 88-89 -8.352626513300073 2313 90-91 -7.829729093061549 2313 92-93 -7.264986232741265 2313 94-95 -6.75521549790132 2313 96-97 -6.124300427431346 2313 98-99 -5.426414536838976 2313 100-101 -4.595274169498798 2314 1 -20.12013975471132 2314 2 -19.016511136274705 2314 3 -19.45005702497835 2314 4 -21.338297046212563 2314 5 -21.275098448418266 2314 6 -21.957980824181234 2314 7 -21.98503112147577 2314 8 -22.701520705521826 2314 9 -23.726033303194196 2314 10-11 -23.911772512071465 2314 12-13 -23.79897086571081 2314 14-15 -23.97649918626927 2314 16-17 -23.104230765818674 2314 18-19 -22.42783623731637 2314 20-21 -21.859754050396166 2314 22-23 -21.71242224326628 2314 24-25 -21.396217425998653 2314 26-27 -20.99625764406567 2314 28-29 -20.826310737039318 2314 30-31 -20.66772730186888 2314 32-33 -20.275562591240227 2314 34-35 -17.590511752644378 2314 36-37 -15.992612448050718 2314 38-39 -15.05229041005347 2314 40-41 -16.109552861879198 2314 42-43 -15.956553101327518 2314 44-45 -14.143076893190521 2314 46-47 -14.740614964839803 2314 48-49 -14.185305241561274 2314 50-51 -12.395950584069007 2314 52-53 -14.873486317213242 2314 54-55 -16.20240823214485 2314 56-57 -16.27644671014684 2314 58-59 -15.88885787662073 2314 60-61 -15.499957642506022 2314 62-63 -14.98149396043327 2314 64-65 -14.451912002821246 2314 66-67 -14.013384786184194 2314 68-69 -13.509212033394194 2314 70-71 -12.970697295023347 2314 72-73 -12.480387818916938 2314 74-75 -11.942165470806012 2314 76-77 -11.381350725779358 2314 78-79 -10.890036419822678 2314 80-81 -10.398309076520215 2314 82-83 -9.954007720615346 2314 84-85 -9.454859498945739 2314 86-87 -8.937736076441192 2314 88-89 -8.429457723491156 2314 90-91 -7.906162214080657 2314 92-93 -7.335448016180756 2314 94-95 -6.75521549790132 2314 96-97 -6.130669854182939 2314 98-99 -5.442338103717957 2314 100-101 -4.61836334147332 2315 1 -20.12013975471132 2315 2 -19.016511136274705 2315 3 -19.45005702497835 2315 4 -17.110419207528146 2315 5 -21.275098448418266 2315 6 -21.957980824181234 2315 7 -21.98503112147577 2315 8 -22.701520705521826 2315 9 -19.838797594501948 2315 10-11 -18.38827995138235 2315 12-13 -15.510795454590998 2315 14-15 -15.41541852847757 2315 16-17 -19.459751517580614 2315 18-19 -22.42783623731637 2315 20-21 -21.859754050396166 2315 22-23 -21.71242224326628 2315 24-25 -21.396217425998653 2315 26-27 -20.99625764406567 2315 28-29 -20.826310737039318 2315 30-31 -20.66772730186888 2315 32-33 -20.275562591240227 2315 34-35 -17.590511752644378 2315 36-37 -15.992612448050718 2315 38-39 -15.05229041005347 2315 40-41 -16.109552861879198 2315 42-43 -15.956553101327518 2315 44-45 -14.143076893190521 2315 46-47 -14.740614964839803 2315 48-49 -14.185305241561274 2315 50-51 -12.395950584069007 2315 52-53 -14.873486317213242 2315 54-55 -16.20240823214485 2315 56-57 -16.27644671014684 2315 58-59 -15.88885787662073 2315 60-61 -15.499957642506022 2315 62-63 -14.98149396043327 2315 64-65 -14.451912002821246 2315 66-67 -14.013384786184194 2315 68-69 -13.509212033394194 2315 70-71 -12.970697295023347 2315 72-73 -12.480387818916938 2315 74-75 -11.942165470806012 2315 76-77 -11.381350725779358 2315 78-79 -10.890036419822678 2315 80-81 -10.398309076520215 2315 82-83 -9.954007720615346 2315 84-85 -9.454859498945739 2315 86-87 -8.937736076441192 2315 88-89 -8.429457723491156 2315 90-91 -7.906162214080657 2315 92-93 -7.335448016180756 2315 94-95 -6.75521549790132 2315 96-97 -6.130669854182939 2315 98-99 -5.442338103717957 2315 100-101 -4.61836334147332 2316 1 -20.12013975471132 2316 2 -19.016511136274705 2316 3 -19.45005702497835 2316 4 -21.338297046212563 2316 5 -21.275098448418266 2316 6 -21.957980824181234 2316 7 -21.98503112147577 2316 8 -22.701520705521826 2316 9 -23.726033303194196 2316 10-11 -23.911772512071465 2316 12-13 -23.79897086571081 2316 14-15 -23.97649918626927 2316 16-17 -23.104230765818674 2316 18-19 -22.42783623731637 2316 20-21 -21.859754050396166 2316 22-23 -21.71242224326628 2316 24-25 -21.396217425998653 2316 26-27 -20.99625764406567 2316 28-29 -20.826310737039318 2316 30-31 -20.66772730186888 2316 32-33 -20.275562591240227 2316 34-35 -17.590511752644378 2316 36-37 -15.992612448050718 2316 38-39 -15.05229041005347 2316 40-41 -16.109552861879198 2316 42-43 -15.956553101327518 2316 44-45 -14.143076893190521 2316 46-47 -14.740614964839803 2316 48-49 -14.185305241561274 2316 50-51 -12.395950584069007 2316 52-53 -14.873486317213242 2316 54-55 -16.20240823214485 2316 56-57 -16.27644671014684 2316 58-59 -15.88885787662073 2316 60-61 -15.499957642506022 2316 62-63 -14.98149396043327 2316 64-65 -14.451912002821246 2316 66-67 -14.013384786184194 2316 68-69 -13.509212033394194 2316 70-71 -12.970697295023347 2316 72-73 -12.480387818916938 2316 74-75 -11.942165470806012 2316 76-77 -11.381350725779358 2316 78-79 -10.890036419822678 2316 80-81 -10.398309076520215 2316 82-83 -9.954007720615346 2316 84-85 -9.454859498945739 2316 86-87 -8.937736076441192 2316 88-89 -8.429457723491156 2316 90-91 -7.906162214080657 2316 92-93 -7.335448016180756 2316 94-95 -6.75521549790132 2316 96-97 -6.130669854182939 2316 98-99 -5.442338103717957 2316 100-101 -4.61836334147332 >>END_MODULE >>Per sequence quality scores fail #Quality Count 2 221969.0 3 14885.0 4 28221.0 5 19942.0 6 18332.0 7 22897.0 8 28323.0 9 33271.0 10 34308.0 11 31480.0 12 26770.0 13 23125.0 14 21557.0 15 22667.0 16 24711.0 17 25891.0 18 27293.0 19 27935.0 20 29250.0 21 30675.0 22 32107.0 23 33305.0 24 34499.0 25 36227.0 26 37879.0 27 39248.0 28 40770.0 29 41496.0 30 41278.0 31 39846.0 32 37585.0 33 34462.0 34 29020.0 35 20590.0 36 10397.0 37 2522.0 38 152.0 39 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.212810013737666 16.679122274720893 12.461288446177969 25.646779265363477 2 17.536700759447513 18.633864666888357 37.09265549716308 26.73677907650105 3 21.054900197115987 22.13446203475264 26.96897022397218 29.841667544159193 4 14.523459641020258 14.806339876141491 33.36191875721633 37.30828172562192 5 16.117211128582966 35.51533652994968 32.15092750413545 16.216524837331896 6 36.34641846507867 32.2218615896511 15.914585795136512 15.517134150133716 7 31.55771123160667 29.704604883040624 19.09379841767331 19.643885467679393 8 28.802474411034375 32.79567849340475 18.27899781533308 20.122849280227793 9 28.1693911778375 14.749489785608924 17.831824580546517 39.249294456007064 10-11 28.08976704859284 23.9325132871392 25.325710516189325 22.652009148078633 12-13 28.299231892617264 21.265693403482288 25.51613296262013 24.918941741280314 14-15 25.27665393236252 22.351963714281688 23.288710405007258 29.082671948348533 16-17 25.791942720491157 24.992965779380132 23.94264496826427 25.272446531864446 18-19 25.75650258586685 24.272806925916335 25.420337510117623 24.550352978099188 20-21 26.48638518714496 23.553156531525055 25.439368406118852 24.52108987521113 22-23 26.171380467597068 23.27830153343671 25.2797941434254 25.270523855540823 24-25 26.037503620389458 23.55968739111724 25.134876073044882 25.267932915448426 26-27 25.860067442680513 23.750762722301648 25.137032456497316 25.252137378520523 28-29 25.67915360038907 24.02873807987683 25.124548390557887 25.167559929176207 30-31 25.939152387765375 23.835167164393773 24.957986532980573 25.267693914860278 32-33 25.779015624012107 23.93661136339124 24.943515904805473 25.34085710779118 34-35 26.051385383318955 23.814753364675145 25.05690111201606 25.07696013998984 36-37 26.567074576143995 23.85225207840867 24.510803436945558 25.06986990850178 38-39 26.33667503193169 23.83587508693617 24.643277420924445 25.184172460207694 40-41 26.40992979086535 23.67401266849446 24.74625692264058 25.16980061799961 42-43 26.391274868870518 23.759538949134885 24.553921159268143 25.29526502272646 44-45 26.53336376729934 24.044535746342472 24.341107296224113 25.080993190134077 46-47 26.091004523398 23.916426973623327 24.489213111965082 25.503355391013592 48-49 26.33578951629561 24.109136315559482 24.13913364797663 25.415940520168274 50-51 26.25386157707532 24.26032564501011 24.261567143333053 25.22424563458152 52-53 25.757685003823273 24.01459502033337 24.48812949062093 25.739590485222426 54-55 25.574676225078814 23.803308342971093 24.67147829738796 25.950537134562136 56-57 25.6042144618796 24.04286669386855 24.413534999471935 25.93938384477991 58-59 25.67667665594952 24.07305792529121 24.407325557755875 25.842939861003394 60-61 25.767980538463874 24.021630160707232 24.393427758862032 25.816961541966858 62-63 25.574644303411258 23.987997590921005 24.393887219032283 26.04347088663545 64-65 25.563235611245418 23.8542813473012 24.387345311193588 26.1951377302598 66-67 25.62921787362088 24.06602320938474 24.167563107417887 26.13719580957649 68-69 25.573350175741766 24.008649735037295 24.239215419256226 26.17878466996471 70-71 25.43329893224125 23.902211544994177 24.22351435579295 26.440975166971626 72-73 25.733927481494057 23.991087777589108 23.980807974190352 26.29417676672648 74-75 25.717836629302447 24.04149899003631 23.967462543161137 26.273201837500103 76-77 25.826159183165025 23.83684077224791 24.00438699914825 26.332613045438812 78-79 25.81661105143724 24.00582967054788 23.78421537270824 26.393343905306644 80-81 25.837923069679675 24.068374300922994 23.780711844566262 26.31299078483107 82-83 26.033426955449645 24.0345758509433 23.850071408623073 26.081925784983984 84-85 26.05958269971292 23.972763697996246 23.631969673324065 26.33568392896677 86-87 25.888725687735363 24.10725359717705 23.721673835048914 26.28234688003867 88-89 25.978854635309844 23.991146047751528 23.56354039938783 26.4664589175508 90-91 25.979465300559696 24.031443085160824 23.473589137223 26.51550247705648 92-93 26.059192439901967 23.78026927847484 23.71744895332344 26.443089328299752 94-95 26.022124983460017 23.59846619227546 23.78876788155902 26.5906409427055 96-97 25.975434814211383 23.700128823681617 23.644219894435896 26.68021646767111 98-99 25.80576244028367 23.597838913704912 23.534248013640315 27.0621506323711 100-101 26.622553046453167 23.47051411267294 23.059775889529234 26.84715695134465 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 13250.0 1 14965.5 2 11165.0 3 5383.5 4 6754.5 5 9572.5 6 10853.5 7 10630.5 8 9898.0 9 9210.5 10 8594.0 11 7787.5 12 6985.5 13 6156.5 14 5309.0 15 4475.0 16 3748.5 17 3221.0 18 2775.5 19 2490.0 20 2348.0 21 2375.0 22 2608.0 23 2944.0 24 3389.5 25 3908.0 26 4413.0 27 4869.0 28 5242.0 29 5724.5 30 6270.5 31 6843.0 32 7435.5 33 8096.0 34 8736.5 35 9416.5 36 10299.5 37 11289.0 38 12118.0 39 13190.0 40 14510.5 41 16125.0 42 18338.5 43 20960.5 44 24023.0 45 27796.0 46 32501.5 47 37832.0 48 43226.5 49 48447.5 50 52432.5 51 55043.5 52 57125.0 53 57954.5 54 57053.5 55 54452.0 56 50536.0 57 46037.0 58 41318.5 59 36447.0 60 31550.0 61 26527.5 62 21647.5 63 17310.0 64 13646.0 65 10610.0 66 8167.0 67 6287.5 68 4802.5 69 3639.5 70 2752.5 71 2081.0 72 1570.0 73 1204.5 74 904.0 75 642.0 76 451.5 77 294.5 78 192.0 79 127.0 80 77.0 81 49.5 82 31.0 83 15.5 84 10.5 85 8.0 86 4.5 87 1.5 88 1.5 89 1.5 90 0.0 91 0.5 92 0.5 93 0.5 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 14.245325687451729 2 21.192094611253616 3 17.041014429097892 4 22.22133325060455 5 22.070217146738553 6 15.987610275568501 7 14.795907537517778 8 11.919721508777144 9 3.0329353098982272 10-11 6.4375786807915185 12-13 3.512163580937328 14-15 7.3389686876982845 16-17 11.3579957645038 18-19 14.416974314344355 20-21 14.83272729053969 22-23 12.814090454132057 24-25 15.296076532836524 26-27 12.830949165881558 28-29 12.87715754772281 30-31 13.722256601838865 32-33 12.193951110552328 34-35 19.006993303866647 36-37 23.845566036349506 38-39 22.819143985644377 40-41 17.963385980409605 42-43 17.88003128454403 44-45 23.279431718543602 46-47 12.663627472270889 48-49 21.34623140439192 50-51 21.088656413739727 52-53 11.116014061716765 54-55 12.714693449023013 56-57 12.262814661935886 58-59 13.259519661421553 60-61 12.661055804376897 62-63 12.635257485186377 64-65 13.683069281549468 66-67 12.040059238165837 68-69 13.07170626490955 70-71 11.677862266366013 72-73 11.05172236436697 74-75 11.12209626038668 76-77 11.195041824300384 78-79 11.605079982953516 80-81 11.566137583415927 82-83 10.109389771782844 84-85 10.021667322510012 86-87 10.15078137883852 88-89 11.554381387329107 90-91 9.067292792961958 92-93 11.043517519181377 94-95 11.15320119586639 96-97 10.706547384817853 98-99 10.580862219014668 100-101 10.655277307439222 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1224886.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 98.00746277406608 #Duplication Level Percentage of deduplicated Percentage of total 1 98.94948548845207 96.9778801552246 2 0.8170834237318965 1.6016054646942064 3 0.12938615529786823 0.3804242639650606 4 0.04279781173636884 0.16778019762254634 5 0.021773420727376275 0.10669788607012047 6 0.009544514621172735 0.05612601968586698 7 0.00721338171442941 0.04948756678964486 8 0.0038687409491522545 0.030333238764523575 9 0.003572007976529794 0.03150750949095698 >10 0.013419436060223736 0.24519487453454428 >50 0.0013517337874979587 0.0841502242059049 >100 4.199055340765013E-4 0.08732766998574334 >500 0.0 0.0 >1k 8.397941126572586E-5 0.18148492896627016 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 2205 0.18001675257942373 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 1.632805012058265E-4 2 0.0 0.0 0.0 0.0 1.632805012058265E-4 3 0.0 0.0 0.0 0.0 1.632805012058265E-4 4 0.0 0.0 0.0 0.0 1.632805012058265E-4 5 0.0 0.0 0.0 0.0 1.632805012058265E-4 6 0.0 0.0 0.0 0.0 1.632805012058265E-4 7 0.0 0.0 0.0 0.0 1.632805012058265E-4 8 0.0 0.0 0.0 0.0 1.632805012058265E-4 9 0.0 0.0 0.0 0.0 1.632805012058265E-4 10-11 0.0 0.0 0.0 8.164025060291326E-5 2.4492075180873975E-4 12-13 8.164025060291326E-5 0.0 0.0 1.632805012058265E-4 5.306616289189361E-4 14-15 8.164025060291326E-5 0.0 0.0 1.632805012058265E-4 6.53122004823306E-4 16-17 8.164025060291326E-5 0.0 0.0 1.632805012058265E-4 8.164025060291326E-4 18-19 8.164025060291326E-5 8.164025060291326E-5 0.0 2.0410062650728315E-4 0.0010205031325364157 20-21 8.164025060291326E-5 1.632805012058265E-4 0.0 2.4492075180873975E-4 0.0011429635084407854 22-23 8.164025060291326E-5 1.632805012058265E-4 0.0 5.714817542203927E-4 0.0011837836337422422 24-25 8.164025060291326E-5 1.632805012058265E-4 0.0 0.0012246037590436989 0.0012246037590436987 26-27 8.164025060291326E-5 1.632805012058265E-4 0.0 0.002734948395197594 0.001306244009646612 28-29 8.164025060291326E-5 2.0410062650728315E-4 0.0 0.00665368042413743 0.001306244009646612 30-31 8.164025060291326E-5 2.4492075180873975E-4 0.0 0.01947119976879481 0.001306244009646612 32-33 8.164025060291326E-5 2.4492075180873975E-4 0.0 0.046575762968962015 0.0013878842602495253 34-35 8.164025060291326E-5 2.4492075180873975E-4 0.0 0.08147697010170743 0.0014695245108524385 36-37 8.164025060291326E-5 2.4492075180873975E-4 0.0 0.135522816000836 0.0014695245108524385 38-39 8.164025060291326E-5 2.4492075180873975E-4 0.0 0.2076111572832084 0.001632805012058265 40-41 8.164025060291326E-5 2.8574087711019636E-4 0.0 0.2754542055342293 0.001632805012058265 42-43 8.164025060291326E-5 3.26561002411653E-4 0.0 0.3423175707780153 0.001632805012058265 44-45 8.164025060291326E-5 3.26561002411653E-4 0.0 0.4207738516074149 0.0017144452626611782 46-47 8.164025060291326E-5 3.26561002411653E-4 0.0 0.5142111184224492 0.0018777257638670048 48-49 8.164025060291326E-5 3.26561002411653E-4 0.0 0.6103425135073794 0.0020410062650728315 50-51 8.164025060291326E-5 3.26561002411653E-4 0.0 0.7201078304429964 0.0021226465156757445 52-53 8.164025060291326E-5 3.26561002411653E-4 0.0 0.8493035270221065 0.0024083873927859406 54-55 8.164025060291326E-5 3.26561002411653E-4 0.0 0.9857243857795746 0.0024492075180873973 56-57 8.164025060291326E-5 4.0820125301456624E-4 0.0 1.1370854103973758 0.002490027643388854 58-59 8.164025060291326E-5 4.0820125301456624E-4 0.0 1.297916704085115 0.0025308477686903108 60-61 8.164025060291326E-5 4.898415036174795E-4 0.0 1.4606665436620225 0.002612488019293224 62-63 8.164025060291326E-5 5.306616289189361E-4 0.0 1.6167627028147926 0.002612488019293224 64-65 8.164025060291326E-5 5.714817542203927E-4 0.0 1.7674706054277705 0.002612488019293224 66-67 8.164025060291326E-5 5.714817542203927E-4 0.0 1.9337309757806032 0.002653308144594681 68-69 8.164025060291326E-5 5.714817542203927E-4 0.0 2.1155846339985924 0.002734948395197594 70-71 8.164025060291326E-5 5.714817542203927E-4 0.0 2.3077657839178505 0.0027757685204990506 72-73 8.164025060291326E-5 5.714817542203927E-4 0.0 2.4991305313310788 0.002939049021704877 74-75 8.164025060291326E-5 5.714817542203927E-4 0.0 2.6851478423298167 0.003061509397609247 76-77 8.164025060291326E-5 5.714817542203927E-4 0.0 2.8879422248274533 0.0031023295229107035 78-79 8.164025060291326E-5 5.714817542203927E-4 0.0 3.1051869316818053 0.00314314964821216 80-81 8.164025060291326E-5 5.714817542203927E-4 0.0 3.3394944509121665 0.0033472502747194433 82-83 1.632805012058265E-4 5.714817542203927E-4 0.0 3.5805781109425694 0.0035513509012267265 84-85 1.632805012058265E-4 5.714817542203927E-4 0.0 3.8014966290740526 0.003592171026528183 86-87 1.632805012058265E-4 5.714817542203927E-4 0.0 4.034130523167054 0.0036329911518296395 88-89 1.632805012058265E-4 6.53122004823306E-4 0.0 4.2820311441228 0.003673811277131096 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1125 0.0 55.022083 1 GTATCAA 1875 0.0 41.333664 1 TCAACGC 2495 0.0 31.240194 4 TATCAAC 2520 0.0 30.943777 2 ATCAACG 2540 0.0 30.693424 3 CAACGCA 2540 0.0 29.297655 5 GTACATG 3205 0.0 27.949654 1 GTATTAA 265 0.0 26.586836 1 AACGCAG 2830 0.0 26.398966 6 TACATGG 3345 0.0 26.169493 2 GTGGTAT 500 0.0 25.162539 1 ACATGGG 3625 0.0 24.004099 3 CTAGTAC 290 4.9112714E-11 22.547205 3 TAGTACT 335 0.0 22.516403 4 ACGCAGA 3260 0.0 22.511286 7 GTATAAG 315 5.456968E-12 22.366701 1 CATGGGG 3040 0.0 21.834982 4 CGCAGAG 3525 0.0 21.80739 8 TATAAGG 300 7.6397555E-11 21.800392 2 CCTAGTA 305 9.640644E-11 21.443007 2 >>END_MODULE