FastQCFastQC Report
Sat 18 Jun 2016
SRR1930903_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1930903_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1224886
Sequences flagged as poor quality0
Sequence length101
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGT21090.17217928852154404No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17190.14033959078640787No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16920.13813530402012922No Hit
ATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTT14660.11968460738387082RNA PCR Primer, Index 46 (95% over 21bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA15250.052.014181
GTATCAA24900.042.538351
TCAACGC31900.032.5950134
CAACGCA32200.032.1320725
ATCAACG32150.032.0488133
TATCAAC32950.031.4160822
AACGCAG34750.030.318026
GTGGTAT6650.027.8559571
CTAGTAC3650.027.3186743
GTACCGT1955.4023985E-1026.770766
GTACATG42600.025.9789431
ACGCAGA41500.024.8157
TACATGG44700.024.1140022
CGCAGAG43850.023.485128
ACATGGG46050.023.406123
GGACCGT2554.965841E-1022.3328296
TAGTACT4100.022.0022684
CCTAGTA4350.021.8319052
TGGTATC7500.021.526262
GTATAGT1553.90797E-521.45071
Produced by FastQC (version 0.11.5)