##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1930903_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1224886 Sequences flagged as poor quality 0 Sequence length 101 %GC 51 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.19160068773747 31.0 31.0 33.0 30.0 34.0 2 31.288123956025295 31.0 31.0 34.0 28.0 34.0 3 31.37633298119172 31.0 31.0 34.0 28.0 34.0 4 35.24573143949723 35.0 35.0 37.0 33.0 37.0 5 35.0093029065562 35.0 35.0 37.0 32.0 37.0 6 35.053564984823076 36.0 35.0 37.0 32.0 37.0 7 34.92265076096878 36.0 35.0 37.0 32.0 37.0 8 34.898995498356584 36.0 35.0 37.0 32.0 37.0 9 36.391233959731764 38.0 35.0 39.0 32.0 39.0 10-11 36.31213680293513 38.0 35.0 39.0 32.0 39.0 12-13 36.18024656988487 38.0 35.0 39.0 31.5 39.0 14-15 37.1789370602652 39.0 36.0 40.0 31.0 41.0 16-17 37.11470985871338 39.0 36.0 40.0 31.0 41.0 18-19 37.07165319874666 39.0 36.0 40.0 31.0 41.0 20-21 37.01660889258265 39.0 36.0 40.0 31.0 41.0 22-23 36.86273416464879 39.0 36.0 40.0 31.0 41.0 24-25 36.71123925002 38.0 35.0 40.0 30.0 41.0 26-27 36.39684142034442 38.0 35.0 40.0 30.0 41.0 28-29 36.17606781365776 38.0 35.0 40.0 30.0 41.0 30-31 35.90799796879057 38.0 34.0 40.0 29.0 41.0 32-33 35.640706155511616 38.0 34.0 40.0 28.0 41.0 34-35 35.855200402323156 38.0 34.0 40.0 29.0 41.0 36-37 35.8976912953532 38.0 34.0 40.0 29.0 41.0 38-39 35.81253969757185 38.0 34.0 40.0 29.0 41.0 40-41 35.68635081142246 38.0 34.0 40.0 28.0 41.0 42-43 35.49569102757318 38.0 34.0 40.0 27.5 41.0 44-45 35.26305590887642 38.0 34.0 40.0 27.0 41.0 46-47 34.97423474511098 38.0 33.0 40.0 26.0 41.0 48-49 34.7094243872491 38.0 33.0 40.0 26.0 41.0 50-51 34.351084509089006 37.0 33.0 40.0 25.0 41.0 52-53 34.05351926628274 37.0 32.5 40.0 24.0 41.0 54-55 33.786420532196466 36.5 32.0 40.0 24.0 41.0 56-57 33.425124460562046 36.0 32.0 39.0 23.0 41.0 58-59 33.0836898290943 36.0 31.0 39.0 22.5 40.5 60-61 32.66439856443783 35.0 31.0 39.0 22.0 40.0 62-63 32.253760758144026 35.0 31.0 38.0 21.5 40.0 64-65 31.840344734122198 35.0 30.5 37.5 20.0 40.0 66-67 31.408911114993558 34.5 30.0 37.0 19.5 39.5 68-69 30.949591635466483 34.0 30.0 36.5 18.0 39.0 70-71 30.505028223034635 34.0 29.0 36.0 17.5 39.0 72-73 30.064197402860348 34.0 29.0 35.5 15.0 37.5 74-75 29.541468348891243 33.5 29.0 35.0 11.0 37.0 76-77 28.256023417689484 31.5 26.5 34.0 8.0 36.0 78-79 29.00896818152873 33.0 28.5 35.0 8.0 36.0 80-81 29.03638420228495 33.0 29.0 35.0 7.0 36.0 82-83 28.810840355755555 33.0 29.0 35.0 7.0 35.0 84-85 28.54637900996501 33.0 28.0 35.0 2.0 35.0 86-87 28.24143961152303 33.0 27.0 35.0 2.0 35.0 88-89 27.913646249528526 33.0 27.0 35.0 2.0 35.0 90-91 27.572288359896348 33.0 26.5 35.0 2.0 35.0 92-93 27.19430869484997 33.0 25.5 35.0 2.0 35.0 94-95 26.7406501502997 32.5 25.0 35.0 2.0 35.0 96-97 26.226154515603902 32.0 24.5 34.0 2.0 35.0 98-99 25.66613137875688 32.0 23.5 34.0 2.0 35.0 100-101 24.380554190349145 31.0 18.0 34.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.5494361998774444 1101 2 0.5833876405364684 1101 3 0.5815018135024275 1101 4 0.4430093840803835 1101 5 0.48567464026393736 1101 6 0.3607754282470097 1101 7 0.5059950686570005 1101 8 0.4769471016650044 1101 9 0.718373196616497 1101 10-11 0.6999337319094749 1101 12-13 0.6856024087256998 1101 14-15 0.7535813426035105 1101 16-17 0.7458275384938133 1101 18-19 0.7928446932914426 1101 20-21 0.6734314139271689 1101 22-23 0.7641994224856319 1101 24-25 0.7734244044412151 1101 26-27 0.8620659012748249 1101 28-29 0.8345207910552475 1101 30-31 0.801878114839937 1101 32-33 0.6903247403182249 1101 34-35 0.5587792471994248 1101 36-37 0.9187501763536758 1101 38-39 0.9362109042086502 1101 40-41 0.9389526797089687 1101 42-43 0.7085271797837223 1101 44-45 0.7669963097253216 1101 46-47 0.8591523725109198 1101 48-49 0.9278747535241436 1101 50-51 0.9812657569789351 1101 52-53 0.8925313348958426 1101 54-55 0.7459716476396636 1101 56-57 0.8751760879204724 1101 58-59 0.6477274889181288 1101 60-61 0.5001844555116364 1101 62-63 0.719007242283844 1101 64-65 0.9166573128978612 1101 66-67 0.922577923185333 1101 68-69 0.8843613442467877 1101 70-71 0.7958409758143965 1101 72-73 0.8691182390063439 1101 74-75 0.8245174269630446 1101 76-77 0.9349334904453777 1101 78-79 0.9559336364175834 1101 80-81 0.8873726624572065 1101 82-83 0.9943094058227651 1101 84-85 1.0908065699388736 1101 86-87 1.0807565638741146 1101 88-89 1.0936248347534132 1101 90-91 0.9746431291000341 1101 92-93 1.0747283051045073 1101 94-95 1.1749214398306833 1101 96-97 1.1367583793741431 1101 98-99 1.069960857645686 1101 100-101 1.2641548965068985 1102 1 0.5099489878043713 1102 2 0.511186846255292 1102 3 0.5099987158058426 1102 4 0.246321854294834 1102 5 0.36359171730921247 1102 6 0.5533663734416052 1102 7 0.5673860138515963 1102 8 0.5995434479715911 1102 9 0.6662766120255554 1102 10-11 0.523176623092958 1102 12-13 0.7513101132530977 1102 14-15 0.9197608501491814 1102 16-17 0.7395323836089815 1102 18-19 0.7313123660475966 1102 20-21 0.8022895553092226 1102 22-23 0.7331029967509153 1102 24-25 0.918158637959877 1102 26-27 0.9894816280420926 1102 28-29 1.0037865575365785 1102 30-31 0.9977249774292929 1102 32-33 0.9160664400799376 1102 34-35 0.9023585958888702 1102 36-37 0.9859632025649532 1102 38-39 1.1658035968218314 1102 40-41 0.952481829828109 1102 42-43 0.837739570570065 1102 44-45 0.8774134661987176 1102 46-47 0.9013598387539687 1102 48-49 0.9529409965741777 1102 50-51 0.9577359714348503 1102 52-53 1.020679388906963 1102 54-55 0.811085865272716 1102 56-57 0.848403411192912 1102 58-59 0.7903428979729341 1102 60-61 0.776710905074772 1102 62-63 0.6782927069383291 1102 64-65 0.6818871778700668 1102 66-67 0.7255310605959728 1102 68-69 0.6421087628329651 1102 70-71 0.4597625008342554 1102 72-73 0.6128462771318439 1102 74-75 0.698085020291078 1102 76-77 0.8425830535907295 1102 78-79 1.1715647722396625 1102 80-81 1.1053262764365535 1102 82-83 1.1182480873160152 1102 84-85 1.0423500171191726 1102 86-87 0.9988439348034213 1102 88-89 0.8699333335619919 1102 90-91 1.086451866192963 1102 92-93 1.2272982812760738 1102 94-95 1.054843600275479 1102 96-97 1.2643574262367316 1102 98-99 1.1811792849689589 1102 100-101 1.566806842495776 1103 1 0.32118661309350216 1103 2 0.3792873859623107 1103 3 0.35297419768710725 1103 4 0.35208654203577794 1103 5 0.32564198716271875 1103 6 0.44883591854569715 1103 7 0.4475636545609305 1103 8 0.3268291842861686 1103 9 0.5597668201982628 1103 10-11 0.4148709947197844 1103 12-13 0.4568166668382929 1103 14-15 0.4461602333411676 1103 16-17 0.6003225146806841 1103 18-19 0.47863973690341766 1103 20-21 0.5754050526106838 1103 22-23 0.6429435518781403 1103 24-25 0.6803998098951212 1103 26-27 0.6688638948115653 1103 28-29 0.7058800039513855 1103 30-31 0.7331531963961382 1103 32-33 0.6438302026088465 1103 34-35 0.6947323815480786 1103 36-37 0.7135656697970276 1103 38-39 0.7204006669837426 1103 40-41 0.715862246173522 1103 42-43 0.6800778897682136 1103 44-45 0.7233644299612436 1103 46-47 0.6184811957316114 1103 48-49 0.6298668254099553 1103 50-51 0.7592718234009297 1103 52-53 0.6361248784983289 1103 54-55 0.600985325565702 1103 56-57 0.5697021004758724 1103 58-59 0.5887681871016994 1103 60-61 0.7219349605874967 1103 62-63 0.5876734316877545 1103 64-65 0.5788762295277365 1103 66-67 0.4789666812590454 1103 68-69 0.49358756005733184 1103 70-71 0.39403574678645015 1103 72-73 0.3915133550038554 1103 74-75 0.5353920364822891 1103 76-77 0.4811209101828666 1103 78-79 0.5780976943676492 1103 80-81 0.5434767775930673 1103 82-83 0.7578160132990526 1103 84-85 0.8055916516604213 1103 86-87 0.8024090851503765 1103 88-89 0.8245291701078656 1103 90-91 0.9421823210888753 1103 92-93 1.1380733005513228 1103 94-95 1.2474989587951413 1103 96-97 0.7160477886114442 1103 98-99 0.9230512973051184 1103 100-101 1.1990501732590744 1104 1 0.2247407997831452 1104 2 0.3546840410998726 1104 3 0.30593381815610954 1104 4 0.12235945854047969 1104 5 0.24055790381414255 1104 6 0.20010481165237337 1104 7 0.3149984475134673 1104 8 0.3392395814060123 1104 9 0.47738624053199885 1104 10-11 0.3740372751020544 1104 12-13 0.29864445745324986 1104 14-15 0.5236122527268989 1104 16-17 0.37101805457182735 1104 18-19 0.3807416306419853 1104 20-21 0.23599054090436766 1104 22-23 0.36046190501475905 1104 24-25 0.3598899495595731 1104 26-27 0.5219362148502853 1104 28-29 0.3435821602659175 1104 30-31 0.47431390843762955 1104 32-33 0.5918808449320991 1104 34-35 0.38617785290175277 1104 36-37 0.542878138122191 1104 38-39 0.423494878095525 1104 40-41 0.4995468791078608 1104 42-43 0.45446860771942 1104 44-45 0.562187385834811 1104 46-47 0.5866765938714806 1104 48-49 0.507061997332336 1104 50-51 0.4132204293575228 1104 52-53 0.549796598914547 1104 54-55 0.4690306719444308 1104 56-57 0.6622159964847967 1104 58-59 0.5845773179880993 1104 60-61 0.4109347109883501 1104 62-63 0.48293531497471776 1104 64-65 0.329407723738143 1104 66-67 0.2819844343639808 1104 68-69 0.3544939030907379 1104 70-71 0.21319297846882534 1104 72-73 0.2543340709150108 1104 74-75 0.17953642286878946 1104 76-77 0.34098881553159544 1104 78-79 0.5048903218984933 1104 80-81 0.40257601108401175 1104 82-83 0.49722397814239727 1104 84-85 0.5455485008189456 1104 86-87 0.5747711523925041 1104 88-89 0.675324993910742 1104 90-91 0.6245752930314801 1104 92-93 0.6169953244906274 1104 94-95 0.6819838580465181 1104 96-97 0.5510934383671362 1104 98-99 0.5589776170387104 1104 100-101 0.7710291321091169 1105 1 0.1279948178901087 1105 2 0.3287781167151529 1105 3 0.30871173217373027 1105 4 0.1691396251444104 1105 5 0.2177684904970576 1105 6 0.23063527445486187 1105 7 0.25972085406984036 1105 8 0.28781655787027205 1105 9 0.46011542730224875 1105 10-11 0.36350117492460043 1105 12-13 0.1794769098236344 1105 14-15 0.3978278805466928 1105 16-17 0.3005044802574659 1105 18-19 0.2579845406383967 1105 20-21 0.19393507362565998 1105 22-23 0.2143070497516959 1105 24-25 0.29775739088272246 1105 26-27 0.32734070832268714 1105 28-29 0.47152841256386324 1105 30-31 0.3702157022928958 1105 32-33 0.534721467359752 1105 34-35 0.4498435530205853 1105 36-37 0.4844476920427425 1105 38-39 0.35452035442121144 1105 40-41 0.3088431704360559 1105 42-43 0.24123076308760005 1105 44-45 0.26344433135849243 1105 46-47 0.18623092215225512 1105 48-49 0.15802221247441395 1105 50-51 0.2985683954410874 1105 52-53 0.2714659828274648 1105 54-55 0.27425375303576516 1105 56-57 0.2506571913955611 1105 58-59 0.24732372416155357 1105 60-61 0.3434516689095446 1105 62-63 0.24359683788143371 1105 64-65 0.4681515582285982 1105 66-67 0.3234531182732958 1105 68-69 0.046358178265546 1105 70-71 0.04299211891687449 1105 72-73 0.12027948056130455 1105 74-75 0.5592080132762725 1105 76-77 0.45779770674739595 1105 78-79 0.24177116351012273 1105 80-81 0.255510843856662 1105 82-83 0.4417277443697962 1105 84-85 0.4540348183662495 1105 86-87 0.4895968576405245 1105 88-89 0.5654136141543518 1105 90-91 0.49865264561619327 1105 92-93 0.5514236125309431 1105 94-95 0.5478178793089938 1105 96-97 0.429434277366898 1105 98-99 0.7746414829424602 1105 100-101 1.2609022438987374 1106 1 0.09617608065064331 1106 2 0.2391296164227228 1106 3 0.23623189693217483 1106 4 0.09481622263577805 1106 5 0.24909430482824035 1106 6 0.3509980324979267 1106 7 0.2062633350083729 1106 8 0.25104664431862744 1106 9 0.23863703820516236 1106 10-11 0.31383963679159166 1106 12-13 0.22087175708871598 1106 14-15 0.28496632960123947 1106 16-17 0.22365536686621823 1106 18-19 0.09090384245551064 1106 20-21 0.16878087951013043 1106 22-23 0.11549142901880316 1106 24-25 0.25173672015166204 1106 26-27 0.33857264782900387 1106 28-29 0.42812628356397653 1106 30-31 0.2706357841264051 1106 32-33 0.1828207779794795 1106 34-35 0.1480249581415336 1106 36-37 0.2559820762331384 1106 38-39 0.22946326196642985 1106 40-41 0.17558137320710188 1106 42-43 0.34911978365986585 1106 44-45 0.30190418157162213 1106 46-47 0.09708038669917585 1106 48-49 0.17057691742653702 1106 50-51 0.21732805667077315 1106 52-53 0.06298806470605456 1106 54-55 0.21070169480087486 1106 56-57 0.30745424833141755 1106 58-59 0.21940773815558856 1106 60-61 0.27638152912348346 1106 62-63 -0.03905767357917256 1106 64-65 0.09477728441496325 1106 66-67 0.11097398297040328 1106 68-69 0.19531728642505541 1106 70-71 0.1857357124019856 1106 72-73 0.2116852421242328 1106 74-75 0.2590031329242599 1106 76-77 0.23664941584339516 1106 78-79 0.23471423951515646 1106 80-81 0.18186691570596025 1106 82-83 0.4716234297817685 1106 84-85 0.36768700342054217 1106 86-87 0.41945885108956915 1106 88-89 0.48190410981769816 1106 90-91 0.4022728707591732 1106 92-93 0.4932818429199095 1106 94-95 0.3071175728782194 1106 96-97 0.36596229686078274 1106 98-99 0.40530105057017707 1106 100-101 0.5838047115977538 1107 1 0.07781675145433553 1107 2 0.1569418200772823 1107 3 0.14267126629200888 1107 4 -0.1263387291008229 1107 5 0.004671148872418485 1107 6 0.06354139214018772 1107 7 -0.007394539626339736 1107 8 -0.12972985045772134 1107 9 -0.03975719500510877 1107 10-11 0.22912995144000803 1107 12-13 0.08600121272954198 1107 14-15 0.061525995998103156 1107 16-17 0.07220313903156494 1107 18-19 0.34652223889726486 1107 20-21 0.3139475956981528 1107 22-23 0.283429997498871 1107 24-25 0.1591480171670554 1107 26-27 0.044062630285928606 1107 28-29 0.1417665126599914 1107 30-31 0.23518077398875192 1107 32-33 0.22532717306422256 1107 34-35 -0.02320251106701221 1107 36-37 0.15935766778559213 1107 38-39 0.04783830138428158 1107 40-41 -0.11482377974557068 1107 42-43 0.33071548680042895 1107 44-45 0.2975229650768014 1107 46-47 0.27544689933736777 1107 48-49 0.25493710726977525 1107 50-51 0.1907441988095755 1107 52-53 0.11808312862275017 1107 54-55 0.6767340328119786 1107 56-57 0.19569765203060285 1107 58-59 0.15944506700808603 1107 60-61 0.11833214666041414 1107 62-63 0.15648477874087519 1107 64-65 0.1196065495978118 1107 66-67 0.1250269469086156 1107 68-69 0.3613518443587722 1107 70-71 0.29747409395317703 1107 72-73 0.24404687548636872 1107 74-75 0.41827290361194613 1107 76-77 0.44201132584204217 1107 78-79 0.405070531401968 1107 80-81 0.24277220014635148 1107 82-83 0.4815925899338147 1107 84-85 0.47595689819621256 1107 86-87 0.4187422145341628 1107 88-89 0.3879339319165176 1107 90-91 0.60010212797307 1107 92-93 0.6213440553972411 1107 94-95 0.8731338022201314 1107 96-97 0.7205357358851963 1107 98-99 0.6290237127924456 1107 100-101 1.0008319733858286 1108 1 0.3044857907783509 1108 2 0.3313087793407945 1108 3 0.3388403514935767 1108 4 0.11016423347699345 1108 5 0.16968242362891317 1108 6 0.21302436600666397 1108 7 0.28686854173263754 1108 8 0.1988713290124835 1108 9 0.31361341499953 1108 10-11 0.37873498740523104 1108 12-13 0.3666781429928676 1108 14-15 0.46243743007483573 1108 16-17 0.564846881750249 1108 18-19 0.6248335556386806 1108 20-21 0.7169051686921151 1108 22-23 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-0.6672998322575445 2316 88-89 -0.6266057019248166 2316 90-91 -0.4638112821236895 2316 92-93 -0.8421663229095522 2316 94-95 -0.6153493023632848 2316 96-97 -0.5035399350007559 2316 98-99 -0.43689532831584543 2316 100-101 -0.49336313020668143 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 14.0 4 28.0 5 42.0 6 149.0 7 416.0 8 830.0 9 1394.0 10 2209.0 11 3300.0 12 4462.0 13 5735.0 14 6623.0 15 8074.0 16 8875.0 17 10205.0 18 9863.0 19 9135.0 20 9398.0 21 10131.0 22 11214.0 23 12829.0 24 15366.0 25 18450.0 26 22937.0 27 29062.0 28 35533.0 29 42513.0 30 51047.0 31 60194.0 32 71488.0 33 85797.0 34 104027.0 35 128059.0 36 164534.0 37 185233.0 38 90158.0 39 5561.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.58900870269573 17.65076207786863 12.88632603835457 25.87390318108107 2 17.939957121560212 19.353508733257243 36.086142230364665 26.62039191481788 3 20.836490167007387 22.453414932040992 27.58348736504536 29.126607535906263 4 15.023463442600725 15.498530778515882 33.00864545782706 36.469360321056335 5 15.740252403120014 36.086233106315305 32.38819593326944 15.785318557295247 6 36.32877589124839 32.94918779997763 15.366447244349052 15.35558906442493 7 31.209353360231074 30.00164913306218 18.7787271631809 20.010270343525846 8 28.48534475861427 33.34187834622977 18.134014104169694 20.038762790986265 9 27.448105374704255 14.31904683374616 17.58188108934219 40.65096670220739 10-11 27.595629307543724 24.16661632184546 25.53907057473104 22.698683795879777 12-13 27.840223498350053 21.100290149450643 25.75974417211071 25.299742180088597 14-15 24.871396090245206 22.504765753519717 23.11714977324402 29.506688382991058 16-17 25.338766219876792 25.40146593233983 23.804296889669732 25.45547095811365 18-19 25.36534012144804 24.461827468025596 25.318356157226063 24.854476253300305 20-21 26.058139288064357 23.63023987538432 25.276393068416166 25.035227768135158 22-23 25.782766722780732 23.58815432619852 25.300150381341613 25.32892856967914 24-25 25.735663901769968 23.852540657043956 25.224756710861474 25.187038730324602 26-27 25.55610164703731 24.08455292252377 25.087234032130862 25.272111398308063 28-29 25.417885998323925 24.1503389500488 25.20402927457301 25.22774577705426 30-31 25.71220142854635 24.063172169758367 24.988743165785102 25.23588323591018 32-33 25.778788153454073 23.954150937387467 24.909258084298322 25.357802824860144 34-35 25.684490116356685 23.866735852237912 25.20813847674438 25.240635554661022 36-37 25.68565900333094 23.938712363660112 24.958689830840573 25.416938802168374 38-39 25.49355855267455 23.792698060218143 25.105030696884594 25.608712690222717 40-41 25.720141762793062 23.57724904744449 25.238536881267535 25.464072308494913 42-43 25.619378908560652 23.67680972047987 25.050998309513712 25.652813061445766 44-45 25.50994164747301 23.742424254794877 25.090361913035387 25.657272184696726 46-47 25.80878642845596 23.73587318072041 24.77984124729139 25.67549914353224 48-49 25.58944427820096 23.820509292919972 24.667520972193703 25.922525456685364 50-51 25.45205690081452 23.807321403889297 24.868057208333436 25.87256448696275 52-53 25.62143591976192 23.745110907908025 24.900359925392976 25.733093246937084 54-55 25.465456613519642 23.6353117884647 24.77074707812502 26.128484519890634 56-57 25.43820615529824 23.970293100886213 24.552730889002472 26.038769854813083 58-59 25.387252236141798 24.180462399992162 24.593272875440736 25.8390124884253 60-61 25.526017103903005 24.26011263466189 24.425196869689238 25.78867339174587 62-63 25.372165878130872 24.311554926670055 24.292718325806185 26.02356086939289 64-65 25.171263593313476 24.232455109835485 24.36685080525046 26.229430491600574 66-67 25.19526860312969 24.292051707665514 24.21915033945721 26.293529349747587 68-69 25.283773987677314 24.233060394884916 24.346006078798823 26.137159538638947 70-71 25.18997044314043 24.04026008728957 24.346247537268855 26.423521932301146 72-73 25.333183009851908 24.06417505496202 24.214866175337633 26.387775759848438 74-75 25.240991409324142 24.141402900979347 24.202975275316476 26.414630414380035 76-77 25.329233746443663 24.00231174787868 24.11282377465697 26.555630731020685 78-79 25.24726364828389 24.12871916042214 24.049400476483623 26.574616714810347 80-81 25.214082370585178 24.286031518215268 24.10050298845845 26.399383122741106 82-83 25.433917931954486 24.082567683849764 24.104773832013755 26.378740552182002 84-85 25.350236675086496 24.008968997931234 23.898305638238988 26.742488688743276 86-87 25.144952264945474 24.13077625183078 24.125306355040387 26.598965128183355 88-89 25.15507565602023 24.038114567396477 24.02844019770003 26.77836957888326 90-91 25.191968706957574 24.11649489838824 23.891398577247553 26.800137817406632 92-93 25.154460292283414 23.93535388176791 24.191219249306446 26.718966576642234 94-95 25.104909263692086 23.747048661570062 24.342296476028437 26.805745598709414 96-97 25.04228964981231 23.77200817055628 24.22266235388436 26.96303982574705 98-99 24.822473275063967 23.72314648057044 24.245399163677273 27.20898108068833 100-101 24.857537762697916 23.827319440339753 24.16151380618278 27.15362899077955 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 114.0 1 76.0 2 57.0 3 83.5 4 77.5 5 69.5 6 117.5 7 222.5 8 342.0 9 427.0 10 407.0 11 378.5 12 379.0 13 405.5 14 493.0 15 544.5 16 596.0 17 669.0 18 742.5 19 860.0 20 1000.0 21 1143.0 22 1328.0 23 1544.5 24 1788.5 25 2071.0 26 2458.0 27 2921.5 28 3277.5 29 3691.5 30 4208.0 31 4726.0 32 5454.5 33 6008.5 34 6306.0 35 6797.0 36 7742.5 37 8732.5 38 9482.5 39 10365.5 40 11392.5 41 12688.0 42 14421.0 43 16843.5 44 20254.5 45 24870.0 46 31313.5 47 39142.5 48 47632.0 49 55635.0 50 61883.0 51 67510.5 52 72413.5 53 75511.5 54 76108.0 55 72914.0 56 67478.5 57 61437.0 58 55677.0 59 49317.0 60 42112.5 61 34826.5 62 27835.0 63 21801.0 64 16657.0 65 12511.5 66 9073.5 67 6996.5 68 5437.0 69 3981.5 70 3062.5 71 2264.5 72 1646.5 73 1272.5 74 926.5 75 707.0 76 533.0 77 311.0 78 176.5 79 112.0 80 63.0 81 33.5 82 19.5 83 12.0 84 8.5 85 5.5 86 2.5 87 0.0 88 0.5 89 1.0 90 1.0 91 1.0 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.016981172125405956 2 0.002285927016881571 3 0.0024492075180873978 4 0.03494202725804687 5 0.0016328050120582647 6 2.4492075180873975E-4 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 8.164025060291326E-5 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 4.898415036174795E-4 26-27 0.0016328050120582647 28-29 4.082012530145663E-5 30-31 0.006082198669917037 32-33 0.021144824906154533 34-35 0.013103260221767577 36-37 4.898415036174795E-4 38-39 4.898415036174795E-4 40-41 0.0018777257638670048 42-43 0.0073884426795636495 44-45 0.007265982303659279 46-47 0.0071843420530563655 48-49 0.008939607441019001 50-51 0.17936363057460042 52-53 0.049351531489461066 54-55 0.01645051049648702 56-57 0.009796830072349591 58-59 0.018940538139875875 60-61 0.05408666602443003 62-63 0.098335681851209 64-65 0.10290753588497216 66-67 0.10735692954283094 68-69 0.10523428302715518 70-71 0.09331480643912984 72-73 0.07012897526790247 74-75 0.2242657684062027 76-77 0.2693720068643123 78-79 0.006531220048233059 80-81 5.714817542203927E-4 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.00628629929642432 92-93 0.017838394756736545 94-95 0.0014695245108524385 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1224886.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 64.0843330845472 #Duplication Level Percentage of deduplicated Percentage of total 1 81.14172922317123 51.999136025938405 2 10.694364261148923 13.7068240287789 3 3.3686678107336077 6.476364901027345 4 1.5541913101432254 3.9839725438530897 5 0.8421781615660321 2.69852129111646 6 0.5448423256850475 2.094951424665595 7 0.35581995957166324 1.5961739365124405 8 0.271012082215435 1.38941028373045 9 0.2022440897902403 1.1664609853048946 >10 0.9690308481876136 10.860531712532936 >50 0.040846396528939476 1.7472181478649844 >100 0.014051603827002997 1.4639439051490668 >500 3.832227866489571E-4 0.16216846817667155 >1k 6.387046444149286E-4 0.6543223453488822 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGT 2109 0.17217928852154404 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1719 0.14033959078640787 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1692 0.13813530402012922 No Hit ATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTT 1466 0.11968460738387082 RNA PCR Primer, Index 46 (95% over 21bp) >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 3.26561002411653E-4 2 0.0 0.0 0.0 0.0 3.26561002411653E-4 3 0.0 0.0 0.0 0.0 3.26561002411653E-4 4 0.0 0.0 0.0 0.0 3.26561002411653E-4 5 0.0 0.0 0.0 0.0 3.26561002411653E-4 6 0.0 0.0 0.0 0.0 4.898415036174795E-4 7 0.0 0.0 0.0 0.0 4.898415036174795E-4 8 0.0 0.0 0.0 0.0 4.898415036174795E-4 9 0.0 0.0 0.0 0.0 5.714817542203927E-4 10-11 0.0 0.0 0.0 0.0 5.714817542203927E-4 12-13 0.0 0.0 0.0 0.0 8.980427566320458E-4 14-15 0.0 0.0 0.0 0.0 0.0010205031325364157 16-17 0.0 0.0 0.0 0.0 0.0011837836337422422 18-19 0.0 0.0 0.0 1.2246037590436988E-4 0.001306244009646612 20-21 0.0 0.0 0.0 2.4492075180873975E-4 0.0014695245108524385 22-23 0.0 0.0 0.0 9.79683007234959E-4 0.0015919848867568085 24-25 0.0 0.0 0.0 0.0020818263903742877 0.0017144452626611782 26-27 0.0 0.0 0.0 0.0048984150361747955 0.0017144452626611782 28-29 0.0 0.0 0.0 0.01379720235189234 0.0017144452626611782 30-31 0.0 0.0 0.0 0.04196308880989741 0.0017960855132640916 32-33 0.0 0.0 0.0 0.10188703275243574 0.001959366014469918 34-35 0.0 0.0 0.0 0.1849559877409 0.0020410062650728315 36-37 0.0 0.0 0.0 0.3107227937946878 0.0020410062650728315 38-39 0.0 0.0 0.0 0.4765749628945061 0.0020818263903742877 40-41 0.0 0.0 0.0 0.6365490339509146 0.0021226465156757445 42-43 0.0 0.0 0.0 0.7925227327277804 0.0022042867662786575 44-45 0.0 0.0 0.0 0.9730293268108217 0.002285927016881571 46-47 0.0 0.0 0.0 1.1813344262241547 0.0024492075180873978 48-49 0.0 4.082012530145663E-5 0.0 1.3888231231314587 0.002694128269896137 50-51 0.0 8.164025060291326E-5 0.0 1.5984344665544385 0.0027757685204990506 52-53 0.0 8.164025060291326E-5 0.0 1.7900033145941743 0.003061509397609247 54-55 0.0 8.164025060291326E-5 0.0 1.992267035462892 0.003183969773513617 56-57 0.0 8.164025060291326E-5 0.0 2.2169410051221092 0.0032656100241165303 58-59 0.0 8.164025060291326E-5 0.0 2.457575643774196 0.0033472502747194433 60-61 0.0 8.164025060291326E-5 0.0 2.704374121346803 0.0034288905253223563 62-63 0.0 8.164025060291326E-5 0.0 2.9404777260904282 0.003592171026528183 64-65 0.0 8.164025060291326E-5 0.0 3.174703605070186 0.0036329911518296395 66-67 0.0 8.164025060291326E-5 0.0 3.4304416900838115 0.003714631402432553 68-69 0.0 8.164025060291326E-5 0.0 3.7159376464421996 0.0037554515277340096 70-71 0.0 8.164025060291326E-5 0.0 4.0167003296633315 0.0037962716530354663 72-73 0.0 8.164025060291326E-5 0.0 4.324688175062822 0.004041192404844206 74-75 0.0 8.164025060291326E-5 0.0 4.624103794149006 0.004326933281954402 76-77 0.0 8.164025060291326E-5 0.0 4.953358924830556 0.004408573532557316 78-79 0.0 8.164025060291326E-5 0.0 5.3126984878592785 0.0045310339084616855 80-81 0.0 8.164025060291326E-5 0.0 5.696775046820684 0.004775954660270425 82-83 0.0 8.164025060291326E-5 0.0 6.101792330061736 0.005306616289189361 84-85 0.0 8.164025060291326E-5 0.0 6.4953391580930795 0.005306616289189361 86-87 0.0 8.164025060291326E-5 0.0 6.914398564437834 0.005510716915696644 88-89 0.0 8.164025060291326E-5 0.0 7.367052933905686 0.0057556376675053835 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1525 0.0 52.01418 1 GTATCAA 2490 0.0 42.53835 1 TCAACGC 3190 0.0 32.595013 4 CAACGCA 3220 0.0 32.132072 5 ATCAACG 3215 0.0 32.048813 3 TATCAAC 3295 0.0 31.416082 2 AACGCAG 3475 0.0 30.31802 6 GTGGTAT 665 0.0 27.855957 1 CTAGTAC 365 0.0 27.318674 3 GTACCGT 195 5.4023985E-10 26.77076 6 GTACATG 4260 0.0 25.978943 1 ACGCAGA 4150 0.0 24.815 7 TACATGG 4470 0.0 24.114002 2 CGCAGAG 4385 0.0 23.48512 8 ACATGGG 4605 0.0 23.40612 3 GGACCGT 255 4.965841E-10 22.332829 6 TAGTACT 410 0.0 22.002268 4 CCTAGTA 435 0.0 21.831905 2 TGGTATC 750 0.0 21.52626 2 GTATAGT 155 3.90797E-5 21.4507 1 >>END_MODULE