FastQCFastQC Report
Sat 18 Jun 2016
SRR1930902_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1930902_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2066046
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN37950.18368419677006223No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA17000.054.1418341
GTATCAA33550.049.4711721
ATCAACG41350.039.151073
TCAACGC42000.038.648724
TATCAAC43450.037.8471262
CAACGCA43050.037.2628365
AACGCAG46950.034.9412166
ACGCAGA54450.029.3130847
CGCAGAG56200.027.9511058
GTACATG41800.025.3884941
GCAGAGT61300.024.5680949
TACATGG43150.024.3579332
ACATGGG45200.023.2472253
GTGGTAT8200.021.4676721
AGAGTAC55650.020.55071410-11
CAGAGTA58200.020.14536310-11
GAGTACT38900.019.99738512-13
GCGCGTA550.00472042519.82416286-87
GTGTAGC7650.019.72381
CATGGGG33150.019.707964
Produced by FastQC (version 0.11.5)