##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1930902_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2066046 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 23.137657632017874 30.0 16.0 31.0 2.0 33.0 2 22.022285563825783 30.0 10.0 31.0 2.0 33.0 3 22.489410206742736 30.0 16.0 31.0 2.0 33.0 4 24.48214996181111 33.0 10.0 35.0 2.0 37.0 5 24.449781853840623 33.0 10.0 35.0 2.0 37.0 6 25.203745705565122 33.0 17.0 35.0 2.0 37.0 7 25.283849923961036 32.0 17.0 35.0 2.0 37.0 8 26.066181488698703 33.0 17.0 35.0 2.0 37.0 9 27.242371176634016 34.0 17.0 37.0 2.0 39.0 10-11 27.49461701239953 34.0 18.5 37.0 2.0 39.0 12-13 27.42302954532474 34.0 18.5 37.0 2.0 39.0 14-15 27.652304208134765 34.0 17.5 38.0 2.0 40.0 16-17 26.75464268462561 33.0 14.0 38.0 2.0 40.0 18-19 26.136785676601587 33.0 10.5 38.0 2.0 40.0 20-21 25.631523209066984 32.0 10.0 38.0 2.0 40.0 22-23 25.56500920115041 32.0 10.0 38.0 2.0 40.0 24-25 25.260326246366247 32.0 10.0 38.0 2.0 40.0 26-27 24.92446973591101 32.0 9.0 38.0 2.0 40.0 28-29 24.84517430880048 32.0 8.5 38.0 2.0 40.0 30-31 24.750668910566368 31.0 8.0 38.0 2.0 40.0 32-33 24.44542861097962 31.0 5.0 37.5 2.0 39.5 34-35 21.508218597262598 26.5 2.0 36.0 2.0 39.0 36-37 19.86985647947819 19.0 2.0 35.0 2.0 38.5 38-39 18.933637489194336 19.0 2.0 34.0 2.0 38.0 40-41 20.266031830849847 24.0 2.0 35.0 2.0 38.0 42-43 20.19158503731282 24.0 2.0 35.0 2.0 38.0 44-45 18.205930797281376 18.5 2.0 34.0 2.0 38.0 46-47 19.058362204907347 19.0 2.0 33.0 2.0 38.0 48-49 18.470246306229384 19.5 2.0 34.0 2.0 38.0 50-51 16.461372834873956 11.0 2.0 32.0 2.0 37.5 52-53 19.411250039931346 23.0 2.0 33.5 2.0 38.0 54-55 21.062886547540568 27.0 2.0 35.5 2.0 38.5 56-57 21.278999838338546 28.0 2.0 36.0 2.0 39.0 58-59 20.90330491189451 27.5 2.0 36.0 2.0 39.0 60-61 20.582719600628447 26.5 2.0 35.5 2.0 39.0 62-63 20.10008901060286 26.0 2.0 35.0 2.0 38.5 64-65 19.603023359596058 25.5 2.0 34.0 2.0 38.0 66-67 19.175657511981825 24.5 2.0 34.0 2.0 38.0 68-69 18.712564241067238 22.5 2.0 34.0 2.0 37.0 70-71 18.112846470988544 21.5 2.0 33.5 2.0 36.5 72-73 17.628265295157995 20.0 2.0 33.0 2.0 36.0 74-75 17.078531649343724 18.0 2.0 32.0 2.0 35.5 76-77 16.512726483340643 12.0 2.0 32.0 2.0 35.0 78-79 16.012402918424854 7.5 2.0 31.0 2.0 35.0 80-81 15.501235451679197 6.5 2.0 31.0 2.0 34.0 82-83 15.018901805671316 2.0 2.0 31.0 2.0 34.0 84-85 14.495377160043871 2.0 2.0 30.0 2.0 34.0 86-87 13.937299314729682 2.0 2.0 30.0 2.0 34.0 88-89 13.377102445928116 2.0 2.0 29.0 2.0 34.0 90-91 12.79265805311208 2.0 2.0 29.0 2.0 34.0 92-93 12.155365127397939 2.0 2.0 29.0 2.0 34.0 94-95 11.485832116032267 2.0 2.0 27.0 2.0 33.5 96-97 10.74762710994818 2.0 2.0 25.5 2.0 33.0 98-99 9.93222391950615 2.0 2.0 24.0 2.0 32.5 100-101 8.865859472635169 2.0 2.0 10.5 2.0 31.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 6.635122699239801 1101 2 7.895818902466342 1101 3 7.654595369295695 1101 4 9.542223794563562 1101 5 9.086717837090518 1101 6 8.538976916696289 1101 7 8.360508551138064 1101 8 7.61171741008296 1101 9 7.609265800145245 1101 10-11 6.945268490925088 1101 12-13 6.848574863087634 1101 14-15 7.346121824390792 1101 16-17 8.108675435759142 1101 18-19 8.631122598671336 1101 20-21 9.196376574147905 1101 22-23 9.09196579799286 1101 24-25 8.971376883407569 1101 26-27 8.837753984919804 1101 28-29 8.8540109290368 1101 30-31 8.729004349994323 1101 32-33 8.66176330776688 1101 34-35 11.327687342017747 1101 36-37 5.440688583034262 1101 38-39 8.417182907923976 1101 40-41 9.983964165948539 1101 42-43 10.413068505408294 1101 44-45 4.647567325371924 1101 46-47 6.209402719988109 1101 48-49 9.597590606221736 1101 50-51 -3.3965093920169824 1101 52-53 6.163095628265928 1101 54-55 8.698513034956626 1101 56-57 9.231567745934555 1101 58-59 9.53615543708423 1101 60-61 9.281697892303004 1101 62-63 9.280052629028013 1101 64-65 9.37523121053255 1101 66-67 9.254199751134887 1101 68-69 9.318860777550231 1101 70-71 9.386360925549265 1101 72-73 9.138929546634348 1101 74-75 9.099887450354004 1101 76-77 8.990340770534235 1101 78-79 8.990952517997714 1101 80-81 8.89222605230982 1101 82-83 8.761461328900312 1101 84-85 8.721320036674017 1101 86-87 8.550989821911935 1101 88-89 8.481445117537822 1101 90-91 7.928910536512703 1101 92-93 7.934951030285577 1101 94-95 7.8947202150461475 1101 96-97 7.581011148813936 1101 98-99 7.507276657630902 1101 100-101 7.124833417350613 1102 1 6.310860579680149 1102 2 7.3064416509122765 1102 3 7.22107429658875 1102 4 8.894991823947159 1102 5 8.951879191171649 1102 6 8.036659829636118 1102 7 7.8453438954793135 1102 8 6.981536869182619 1102 9 6.683433193232844 1102 10-11 6.2987330645540425 1102 12-13 6.04679493605915 1102 14-15 6.439290683024325 1102 16-17 7.406933310686515 1102 18-19 7.80524614060381 1102 20-21 8.090066771830422 1102 22-23 7.787921409722021 1102 24-25 8.041199488977181 1102 26-27 8.015078062904397 1102 28-29 7.844792879998845 1102 30-31 7.3357844609362 1102 32-33 7.743519343781987 1102 34-35 9.815994771630994 1102 36-37 0.6960617344277722 1102 38-39 2.953963479757217 1102 40-41 7.706767918845173 1102 42-43 8.663917387547293 1102 44-45 0.9367603754845533 1102 46-47 1.4970640818246217 1102 48-49 6.9694503959755 1102 50-51 -4.830886548290621 1102 52-53 4.292966507775187 1102 54-55 7.00525950969158 1102 56-57 7.441885331111937 1102 58-59 7.7669554337161735 1102 60-61 7.699199001322871 1102 62-63 7.682736080413061 1102 64-65 7.733003678211439 1102 66-67 7.488127604794787 1102 68-69 7.672530979637806 1102 70-71 7.66303590007837 1102 72-73 7.462323174013424 1102 74-75 7.366946967827328 1102 76-77 7.002137152271061 1102 78-79 7.262164267891462 1102 80-81 7.08463446233651 1102 82-83 6.935255599400117 1102 84-85 6.884687304611889 1102 86-87 6.888868733339693 1102 88-89 6.599397080248165 1102 90-91 6.653790184037163 1102 92-93 6.590271422911849 1102 94-95 6.177274102775668 1102 96-97 6.111995790052102 1102 98-99 5.879543826610075 1102 100-101 5.376514784531691 1103 1 6.359439368347839 1103 2 7.461535545972978 1103 3 7.172364369868866 1103 4 8.779558468671347 1103 5 8.929051910753053 1103 6 8.210472658813625 1103 7 7.709000157881174 1103 8 6.929538801154052 1103 9 7.097142524555828 1103 10-11 6.486740672714404 1103 12-13 6.359103329691223 1103 14-15 6.638083050143692 1103 16-17 7.182918401861915 1103 18-19 7.779850578496376 1103 20-21 8.07903362577256 1103 22-23 7.994870391421049 1103 24-25 8.22426594883763 1103 26-27 8.189360318935403 1103 28-29 7.891877470071844 1103 30-31 7.876645699865637 1103 32-33 7.670818044180205 1103 34-35 9.87832852077316 1103 36-37 -3.346433669818733 1103 38-39 -1.9691062876245482 1103 40-41 6.321267696082749 1103 42-43 8.825992176719723 1103 44-45 0.7381847414514517 1103 46-47 0.4701994074658167 1103 48-49 7.007010250590987 1103 50-51 -5.19511290591938 1103 52-53 4.160751142486806 1103 54-55 7.140323951447225 1103 56-57 7.478613137396273 1103 58-59 7.934626523115934 1103 60-61 7.883756429776728 1103 62-63 7.704763612513354 1103 64-65 7.7073973046867685 1103 66-67 7.622277310186373 1103 68-69 7.658431593359584 1103 70-71 7.597310649586387 1103 72-73 7.43716135633235 1103 74-75 7.265033428932405 1103 76-77 7.2644090576552784 1103 78-79 6.880959085505134 1103 80-81 7.047695844634941 1103 82-83 6.785387092752453 1103 84-85 6.799341518977597 1103 86-87 6.558099159693404 1103 88-89 6.64483253933148 1103 90-91 6.622061127742327 1103 92-93 6.422744176236003 1103 94-95 6.201631757803346 1103 96-97 5.990729806121575 1103 98-99 5.629367522373238 1103 100-101 5.428269525796745 1104 1 6.245826227834694 1104 2 7.508752639476633 1104 3 6.999556492157151 1104 4 8.828744106688845 1104 5 8.702187830138435 1104 6 8.016010791449649 1104 7 7.759086879849658 1104 8 6.926854689973254 1104 9 6.798182893705778 1104 10-11 6.250498398920335 1104 12-13 5.973764790095061 1104 14-15 6.508483767477188 1104 16-17 7.216667823960851 1104 18-19 7.763575853901198 1104 20-21 8.070173703100878 1104 22-23 7.9405079377635595 1104 24-25 8.069438524082553 1104 26-27 8.074950631988461 1104 28-29 7.987167834068533 1104 30-31 7.711282807445052 1104 32-33 7.406681921609231 1104 34-35 10.07579694602589 1104 36-37 -3.259261159357621 1104 38-39 -1.8558236917960365 1104 40-41 6.292138537771727 1104 42-43 8.718568098932032 1104 44-45 0.7256601162405367 1104 46-47 -0.04607237241056694 1104 48-49 6.975729975200885 1104 50-51 -5.298524804350139 1104 52-53 3.9820567719057216 1104 54-55 6.874042601234766 1104 56-57 7.44600994708793 1104 58-59 7.3592638073784755 1104 60-61 7.423538844505032 1104 62-63 7.543715469305788 1104 64-65 7.423696470447933 1104 66-67 7.2983089187979076 1104 68-69 7.23536237185489 1104 70-71 7.2874430441433695 1104 72-73 7.314769272914358 1104 74-75 7.154256788797657 1104 76-77 6.9036243195438445 1104 78-79 6.7579022318193775 1104 80-81 6.518059458511633 1104 82-83 6.548172325576646 1104 84-85 6.317118786337451 1104 86-87 6.2114579059499295 1104 88-89 5.960055115901531 1104 90-91 5.8510255290368285 1104 92-93 5.907081216970299 1104 94-95 5.682590469901134 1104 96-97 5.202341715742264 1104 98-99 4.634642249913133 1104 100-101 4.294457822895312 1105 1 5.966276752133766 1105 2 6.918160875342107 1105 3 6.819183914307789 1105 4 8.50749081663961 1105 5 8.472874686770169 1105 6 7.699768732353835 1105 7 7.600817503166528 1105 8 6.6281896262769 1105 9 6.832449515997055 1105 10-11 6.250004708841196 1105 12-13 5.907532022269493 1105 14-15 6.240794024079101 1105 16-17 6.916300683070535 1105 18-19 7.509761422073861 1105 20-21 7.919470447899123 1105 22-23 7.585654908955608 1105 24-25 7.694226338612932 1105 26-27 7.818403000761268 1105 28-29 7.690221028991868 1105 30-31 7.233795346484047 1105 32-33 7.217587346500064 1105 34-35 9.418177331882948 1105 36-37 -3.514739146146061 1105 38-39 -2.2632002713641803 1105 40-41 5.718620161309499 1105 42-43 7.712625757423353 1105 44-45 -3.221357198093786 1105 46-47 -4.168478176677535 1105 48-49 4.923320947176329 1105 50-51 -9.72448141892027 1105 52-53 0.46829295557806105 1105 54-55 5.313101994792511 1105 56-57 6.474875000678722 1105 58-59 6.898366692432727 1105 60-61 6.7413722163258 1105 62-63 6.6367476012326385 1105 64-65 6.688362479557135 1105 66-67 6.667490443493268 1105 68-69 6.701675566586136 1105 70-71 6.63741523297038 1105 72-73 6.413745834275783 1105 74-75 6.469802420209415 1105 76-77 6.225674675305537 1105 78-79 5.7517954661460475 1105 80-81 5.8763754168216025 1105 82-83 5.918582281475475 1105 84-85 5.716855331847343 1105 86-87 5.607129781713327 1105 88-89 5.533336408377769 1105 90-91 5.305575453641826 1105 92-93 5.418989702342914 1105 94-95 5.007641801559396 1105 96-97 4.779918393776992 1105 98-99 4.400763334582285 1105 100-101 4.017584546217153 1106 1 5.709490464779986 1106 2 6.878413416769266 1106 3 6.472432013396208 1106 4 7.831661654361355 1106 5 7.617714780734463 1106 6 7.212938508602591 1106 7 6.808608681880283 1106 8 5.985771069153802 1106 9 6.0659001121057585 1106 10-11 5.482547563955912 1106 12-13 5.46347870595055 1106 14-15 5.868165107937116 1106 16-17 6.515903435471646 1106 18-19 6.697623156550431 1106 20-21 7.040294921867449 1106 22-23 6.761014930713326 1106 24-25 7.087383222009382 1106 26-27 7.024601557290072 1106 28-29 6.870787303370253 1106 30-31 6.23988406201919 1106 32-33 6.406894058542083 1106 34-35 8.83918632551411 1106 36-37 -0.16906450412493612 1106 38-39 0.9202912442805804 1106 40-41 6.339708352711401 1106 42-43 7.567208704191231 1106 44-45 -3.5574637259050395 1106 46-47 -4.5209450902508515 1106 48-49 4.373961731015527 1106 50-51 -9.699683313393678 1106 52-53 0.10885001429088703 1106 54-55 4.648726175576385 1106 56-57 5.626450103606935 1106 58-59 5.844512414680079 1106 60-61 6.0281031230185285 1106 62-63 6.030861853199582 1106 64-65 6.078446373891875 1106 66-67 5.968140182994919 1106 68-69 5.703070916575076 1106 70-71 5.563100361783345 1106 72-73 5.2102653419372125 1106 74-75 5.22080539249886 1106 76-77 5.104378275069141 1106 78-79 4.878842990586341 1106 80-81 4.693288247273166 1106 82-83 4.658270166321339 1106 84-85 4.65613160701812 1106 86-87 4.476464190276426 1106 88-89 4.472368672699401 1106 90-91 4.347332055244436 1106 92-93 4.27766377730965 1106 94-95 3.9435958148517454 1106 96-97 3.5755897960769047 1106 98-99 3.4675577191755753 1106 100-101 3.2013462793730785 1107 1 5.813034016343028 1107 2 6.930397947212892 1107 3 6.3994060870889555 1107 4 7.718735984164059 1107 5 8.062360763411647 1107 6 6.521459312710643 1107 7 7.027753223134578 1107 8 6.30412765401114 1107 9 6.3481229921201745 1107 10-11 5.788133238528957 1107 12-13 5.481878565669213 1107 14-15 5.753605369323722 1107 16-17 6.689834192476059 1107 18-19 6.909650672283828 1107 20-21 7.35137441247889 1107 22-23 7.299161560100334 1107 24-25 7.201448678052422 1107 26-27 7.257882017001016 1107 28-29 7.240116929115093 1107 30-31 7.048219459890515 1107 32-33 6.695847466477634 1107 34-35 8.882552884503056 1107 36-37 -0.2228689114041238 1107 38-39 0.9775158134624427 1107 40-41 7.011455659695869 1107 42-43 8.145623879028104 1107 44-45 -3.111503812261713 1107 46-47 -4.228789503450166 1107 48-49 4.7815880003042075 1107 50-51 -9.720341026936618 1107 52-53 0.37538535489139235 1107 54-55 5.010035071214794 1107 56-57 6.393329310438251 1107 58-59 6.741012298851452 1107 60-61 6.501527290661155 1107 62-63 6.485829051176445 1107 64-65 6.219128282747615 1107 66-67 6.166424712795052 1107 68-69 6.321590948492297 1107 70-71 6.230565227100392 1107 72-73 6.227631592049821 1107 74-75 6.254593165500495 1107 76-77 6.271917946373243 1107 78-79 6.123561124844587 1107 80-81 6.036377461054194 1107 82-83 5.702802653033219 1107 84-85 5.723401494149565 1107 86-87 5.655403328768854 1107 88-89 5.500368231907137 1107 90-91 5.457497545392563 1107 92-93 5.272288202992721 1107 94-95 5.058899189325691 1107 96-97 4.954095220717516 1107 98-99 4.549778346966724 1107 100-101 4.199025685043532 1108 1 6.37480848801064 1108 2 6.738317904100274 1108 3 6.933793118866298 1108 4 8.56090017469733 1108 5 8.437034914631973 1108 6 7.719428226592196 1108 7 7.938970657917221 1108 8 6.823143605183283 1108 9 5.099327049408302 1108 10-11 6.070981051316878 1108 12-13 5.573903993670399 1108 14-15 6.352867454805576 1108 16-17 6.565348990087646 1108 18-19 7.017618062985303 1108 20-21 7.877228221132711 1108 22-23 7.912309108235721 1108 24-25 7.387584800681662 1108 26-27 7.837544955463716 1108 28-29 7.711400224879416 1108 30-31 7.5512333282794835 1108 32-33 7.504349377489149 1108 34-35 9.983179830386288 1108 36-37 -3.3136804518110274 1108 38-39 -2.0136556403218755 1108 40-41 5.963543782285374 1108 42-43 8.049212064945475 1108 44-45 -3.090954436806932 1108 46-47 -3.885296979863641 1108 48-49 5.577513431533873 1108 50-51 -9.703037825526682 1108 52-53 0.7992788142402851 1108 54-55 5.773827922036688 1108 56-57 6.987905444193327 1108 58-59 7.245941317832429 1108 60-61 7.102882713968629 1108 62-63 7.383483353870403 1108 64-65 7.536525999964272 1108 66-67 7.497218343418918 1108 68-69 7.182683755688327 1108 70-71 7.162593626958337 1108 72-73 7.00186070978916 1108 74-75 7.186326138789383 1108 76-77 7.042891370192304 1108 78-79 6.916385249698996 1108 80-81 6.948135873105862 1108 82-83 7.000522891212226 1108 84-85 6.76762804152253 1108 86-87 6.477763798438117 1108 88-89 6.318360423953651 1108 90-91 6.366775512510088 1108 92-93 6.270597079904041 1108 94-95 6.088703953236921 1108 96-97 6.040756302390594 1108 98-99 5.5367095240620685 1108 100-101 5.092654218676424 1109 1 4.983839517647784 1109 2 6.548197589431137 1109 3 6.384839610254502 1109 4 7.760946025029959 1109 5 7.59653548563843 1109 6 5.404165160308747 1109 7 5.017159272600367 1109 8 3.499181075094004 1109 9 2.9684147976050674 1109 10-11 2.513406088145736 1109 12-13 3.1779950745070806 1109 14-15 3.8522952808629967 1109 16-17 2.8104751634103913 1109 18-19 3.9858450560950196 1109 20-21 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-9.279875408169838 2313 96-97 -8.527541083177926 2313 98-99 -7.6558959621129015 2313 100-101 -6.581180404547938 2314 1 -20.746894427306344 2314 2 -19.60180241056886 2314 3 -20.05139892185559 2314 4 -21.960154892401235 2314 5 -21.946583780416617 2314 6 -22.716018325929785 2314 7 -22.774125131069358 2314 8 -23.61045195242893 2314 9 -24.763236578541722 2314 10-11 -25.034988407267107 2314 12-13 -24.983014099804848 2314 14-15 -25.18646618702691 2314 16-17 -24.28333217603915 2314 18-19 -23.62791641179489 2314 20-21 -23.120226660866365 2314 22-23 -23.04766312684063 2314 24-25 -22.741753468638098 2314 26-27 -22.42413944990417 2314 28-29 -22.352459099507445 2314 30-31 -22.265286801290163 2314 32-33 -21.959787596134174 2314 34-35 -19.16351151621251 2314 36-37 -17.48628572714652 2314 38-39 -16.583530698165465 2314 40-41 -17.90804344584975 2314 42-43 -17.863097051204456 2314 44-45 -15.87693315036547 2314 46-47 -16.665954083056608 2314 48-49 -16.171676758193108 2314 50-51 -14.13223727022821 2314 52-53 -17.018398187147962 2314 54-55 -18.61071627046678 2314 56-57 -18.81172435682472 2314 58-59 -18.441009168053736 2314 60-61 -18.1321936395714 2314 62-63 -17.651916923778835 2314 64-65 -17.166157942654888 2314 66-67 -16.75150909758062 2314 68-69 -16.296029802849386 2314 70-71 -15.690946400806585 2314 72-73 -15.220490053746243 2314 74-75 -14.691739571529915 2314 76-77 -14.135729638599923 2314 78-79 -13.647011325960422 2314 80-81 -13.127754918194462 2314 82-83 -12.66884314303118 2314 84-85 -12.184246090823605 2314 86-87 -11.639543003968177 2314 88-89 -11.102046794926496 2314 90-91 -10.541831613820314 2314 92-93 -9.932318237078837 2314 94-95 -9.279875408169838 2314 96-97 -8.562899412556188 2314 98-99 -7.753665302407159 2314 100-101 -6.689391130698864 2315 1 -20.746894427306344 2315 2 -19.60180241056886 2315 3 -20.05139892185559 2315 4 -16.735548311571478 2315 5 -21.946583780416617 2315 6 -22.716018325929785 2315 7 -22.774125131069358 2315 8 -23.61045195242893 2315 9 -19.948262806486404 2315 10-11 -18.168750925149798 2315 12-13 -14.700229168951246 2315 14-15 -14.665483831280605 2315 16-17 -20.14074753130858 2315 18-19 -23.62791641179489 2315 20-21 -23.120226660866365 2315 22-23 -23.04766312684063 2315 24-25 -22.741753468638098 2315 26-27 -22.42413944990417 2315 28-29 -22.352459099507445 2315 30-31 -22.265286801290163 2315 32-33 -21.959787596134174 2315 34-35 -19.16351151621251 2315 36-37 -17.48628572714652 2315 38-39 -16.583530698165465 2315 40-41 -17.90804344584975 2315 42-43 -17.863097051204456 2315 44-45 -15.87693315036547 2315 46-47 -16.665954083056608 2315 48-49 -16.171676758193108 2315 50-51 -14.13223727022821 2315 52-53 -17.018398187147962 2315 54-55 -18.61071627046678 2315 56-57 -18.81172435682472 2315 58-59 -18.441009168053736 2315 60-61 -18.1321936395714 2315 62-63 -17.651916923778835 2315 64-65 -17.166157942654888 2315 66-67 -16.75150909758062 2315 68-69 -16.296029802849386 2315 70-71 -15.690946400806585 2315 72-73 -15.220490053746243 2315 74-75 -14.691739571529915 2315 76-77 -14.135729638599923 2315 78-79 -13.647011325960422 2315 80-81 -13.127754918194462 2315 82-83 -12.66884314303118 2315 84-85 -12.184246090823605 2315 86-87 -11.639543003968177 2315 88-89 -11.102046794926496 2315 90-91 -10.541831613820314 2315 92-93 -9.932318237078837 2315 94-95 -9.279875408169838 2315 96-97 -8.562899412556188 2315 98-99 -7.753665302407159 2315 100-101 -6.689391130698864 2316 1 -20.746894427306344 2316 2 -19.60180241056886 2316 3 -20.05139892185559 2316 4 -21.960154892401235 2316 5 -21.946583780416617 2316 6 -22.716018325929785 2316 7 -22.774125131069358 2316 8 -23.61045195242893 2316 9 -24.763236578541722 2316 10-11 -25.034988407267107 2316 12-13 -24.983014099804848 2316 14-15 -25.18646618702691 2316 16-17 -24.28333217603915 2316 18-19 -23.62791641179489 2316 20-21 -23.120226660866365 2316 22-23 -23.04766312684063 2316 24-25 -22.741753468638098 2316 26-27 -22.42413944990417 2316 28-29 -22.352459099507445 2316 30-31 -22.265286801290163 2316 32-33 -21.959787596134174 2316 34-35 -19.16351151621251 2316 36-37 -17.48628572714652 2316 38-39 -16.583530698165465 2316 40-41 -17.90804344584975 2316 42-43 -17.863097051204456 2316 44-45 -15.87693315036547 2316 46-47 -16.665954083056608 2316 48-49 -16.171676758193108 2316 50-51 -14.13223727022821 2316 52-53 -17.018398187147962 2316 54-55 -18.61071627046678 2316 56-57 -18.81172435682472 2316 58-59 -18.441009168053736 2316 60-61 -18.1321936395714 2316 62-63 -17.651916923778835 2316 64-65 -17.166157942654888 2316 66-67 -16.75150909758062 2316 68-69 -16.296029802849386 2316 70-71 -15.690946400806585 2316 72-73 -15.220490053746243 2316 74-75 -14.691739571529915 2316 76-77 -14.135729638599923 2316 78-79 -13.647011325960422 2316 80-81 -13.127754918194462 2316 82-83 -12.66884314303118 2316 84-85 -12.184246090823605 2316 86-87 -11.639543003968177 2316 88-89 -11.102046794926496 2316 90-91 -10.541831613820314 2316 92-93 -9.932318237078837 2316 94-95 -9.279875408169838 2316 96-97 -8.562899412556188 2316 98-99 -7.753665302407159 2316 100-101 -6.689391130698864 >>END_MODULE >>Per sequence quality scores fail #Quality Count 2 325262.0 3 20646.0 4 44077.0 5 26063.0 6 22726.0 7 27856.0 8 36126.0 9 42603.0 10 44171.0 11 41631.0 12 37118.0 13 34813.0 14 32937.0 15 35395.0 16 38488.0 17 40733.0 18 42093.0 19 43679.0 20 46343.0 21 48893.0 22 52142.0 23 55281.0 24 59018.0 25 61788.0 26 65895.0 27 69804.0 28 75256.0 29 79272.0 30 82193.0 31 81852.0 32 79826.0 33 77554.0 34 72531.0 35 60531.0 36 41831.0 37 17504.0 38 2100.0 39 15.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.650078831216824 19.62050770341013 13.764611055269599 23.964802410103445 2 15.478475124326451 20.455442206371526 40.5011659994796 23.56491666982242 3 18.838739379686114 25.32605298603827 30.036013250278465 25.799194383997154 4 12.66004559661339 16.634233066940972 38.2855772274094 32.420144109036244 5 13.395076466353139 37.44084337862274 35.38656395886506 13.777516196159068 6 31.32190776204044 37.00208003402337 17.785867117407577 13.890145086528612 7 27.066603203818623 32.422298523358215 22.60626541112733 17.904832861695837 8 25.488177003797734 35.73052421475028 20.64982297103356 18.13147581041842 9 26.18375417829311 16.004519983531072 20.671597376535363 37.14012846164045 10-11 24.918601320648094 26.118099691126268 28.709800612586665 20.25349837563897 12-13 25.344047758932437 23.8753824179563 28.762289925361635 22.018279897749622 14-15 22.574510278416042 25.253043331708763 26.422394357648816 25.750052032226378 16-17 22.354485229215427 28.19340948686807 27.07115355060529 22.38095173331121 18-19 22.26687992038728 27.259533023884664 28.961406911683298 21.512180144044766 20-21 22.9034206187106 26.503207324824196 29.03672251211794 21.55664954434726 22-23 22.62037362574842 26.371241185277572 28.8066772186946 22.201707970279404 24-25 22.531185105051296 26.54745940095265 28.716560181866452 22.2047953121296 26-27 22.45582815623076 26.646080834964874 28.688452294021637 22.20963871478273 28-29 22.396074946906065 26.921679742251825 28.621017401982872 22.061227908859237 30-31 22.61831525009772 26.689868114999506 28.585434255727616 22.106382379175166 32-33 22.503519492446944 26.85559272861339 28.365380922650168 22.275506856289496 34-35 22.861603200058067 26.816847126548588 28.401972936818364 21.919576736574978 36-37 23.299313289773544 26.803087546089838 28.002748355151084 21.89485080898553 38-39 23.065238778879486 26.79373956846969 28.022979087482724 22.118042565168103 40-41 23.036870741871944 26.78732444826808 28.130764858756102 22.045039951103877 42-43 23.121952820859136 26.768572954385444 27.818939304676 22.29053492007942 44-45 23.297077627286335 26.996631162374218 27.637430415970044 22.068860794369407 46-47 22.734542466946873 26.935622388592588 27.873833611561803 22.456001532898735 48-49 23.184343176145084 27.14247841955003 27.32731813431278 22.34586026999211 50-51 23.453366471129307 27.230709721435403 27.204577158068506 22.111346649366787 52-53 22.53606453396653 27.288586747334126 27.660670819305977 22.51467789939337 54-55 22.327068578824356 27.158826825645882 27.87515481629386 22.638949779235897 56-57 22.40159079556246 27.404774582402442 27.629698144071845 22.563936477963253 58-59 22.419045726683343 27.292425545046502 27.771382678951916 22.51714604931824 60-61 22.40323344216064 27.254030842767836 27.842613708270576 22.50012200680095 62-63 22.382747293723124 27.28261112208899 27.74919395266761 22.585447631520275 64-65 22.196968344672012 27.118973561430792 27.784428265104168 22.899629828793028 66-67 22.3240871293892 27.254945856121342 27.6172643000705 22.803702714418954 68-69 22.332571259614284 27.22502362908401 27.63398535024476 22.808419761056946 70-71 22.271882028156536 27.058487899365662 27.53095457195987 23.138675500517934 72-73 22.395850401469648 27.250292432188093 27.42213612132679 22.931721045015465 74-75 22.358638374463286 27.370890618900983 27.402198898382267 22.86827210825346 76-77 22.484430898257273 27.140049476019755 27.34127981082124 23.03423981490173 78-79 22.325615868111974 27.26354198655495 27.330601016212697 23.080241129120378 80-81 22.438118178183476 27.368427933183646 27.18466988917323 23.00878399945965 82-83 22.611969388340526 27.21237763673828 27.233824886423406 22.941828088497786 84-85 22.522112649391428 27.286571527803254 27.13557006866697 23.05574575413835 86-87 22.413506840459902 27.338235629401435 27.08116684123776 23.1670906889009 88-89 22.52104592531283 27.195746163815166 27.003283491064327 23.279924419807674 90-91 22.49837895327303 27.20837462164531 27.025513128225192 23.26773329685647 92-93 22.574136982265188 27.040987655390854 27.105034252065742 23.27984111027822 94-95 22.43661975268666 26.983470951109823 27.087094465538875 23.492814830664642 96-97 22.530333358611696 26.84429708029532 27.005089046850163 23.62028051424283 98-99 22.378235406473763 26.87063384965855 26.92400221232203 23.82712853154566 100-101 22.754742487487686 26.94155274591185 26.734461698135803 23.569243068464658 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 23336.0 1 25260.5 2 18792.0 3 11037.5 4 14321.5 5 18214.0 6 19303.5 7 18651.0 8 17407.5 9 15865.5 10 14288.5 11 12632.5 12 10851.0 13 9320.0 14 8000.5 15 6843.0 16 6171.0 17 5823.5 18 5632.5 19 5760.5 20 6110.0 21 6767.0 22 7735.5 23 8983.5 24 10622.0 25 12440.5 26 14603.0 27 17112.0 28 20094.0 29 23363.0 30 27022.0 31 31068.5 32 35214.5 33 38745.0 34 41804.5 35 44932.0 36 48368.5 37 51711.0 38 54623.0 39 57096.5 40 58739.5 41 60823.5 42 62631.0 43 64102.5 44 65466.0 45 66497.5 46 67660.0 47 68209.5 48 67999.0 49 66666.5 50 64470.0 51 62302.0 52 59953.0 53 56966.5 54 53674.0 55 49233.5 56 43710.0 57 38389.5 58 33378.5 59 28594.5 60 23846.5 61 19482.0 62 15753.0 63 12465.5 64 9566.5 65 7237.0 66 5560.0 67 4365.5 68 3382.5 69 2591.0 70 1985.5 71 1513.0 72 1172.5 73 927.0 74 719.0 75 523.0 76 392.0 77 301.5 78 201.0 79 116.5 80 73.0 81 54.5 82 34.5 83 21.0 84 14.0 85 7.0 86 5.0 87 3.5 88 2.0 89 1.5 90 1.5 91 0.5 92 0.5 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 13.949205390393049 2 20.75578181705538 3 16.52446266927261 4 21.702420952873265 5 21.54535765418582 6 15.554493946407778 7 14.376833816865645 8 11.537932843702416 9 2.8965473179203176 10-11 6.212881997787077 12-13 3.405369483544897 14-15 7.093864318606652 16-17 11.029449489507979 18-19 14.009634829040593 20-21 14.404035534542794 22-23 12.373950047578806 24-25 14.857026416643194 26-27 12.472253763952981 28-29 12.461435999004863 30-31 13.258005872086102 32-33 11.778053344407628 34-35 18.644381586857214 36-37 23.285928774093122 38-39 22.440570055071376 40-41 17.52262534328858 42-43 17.43257410532002 44-45 22.70307631098243 46-47 12.220855682787315 48-49 20.94527420977074 50-51 20.542427419331418 52-53 10.717863977859157 54-55 12.285181452881494 56-57 11.825293338096055 58-59 12.79347120054442 60-61 12.227341501592898 62-63 12.187119744671707 64-65 13.231167166655533 66-67 11.570991158957739 68-69 12.584860162842453 70-71 11.26765812571453 72-73 10.643107655879879 74-75 10.720284059503031 76-77 10.800001548852252 78-79 11.18539955063924 80-81 11.14244310146047 82-83 9.728970216539224 84-85 9.624446890340293 86-87 9.774879165323522 88-89 11.112337285810673 90-91 8.70837338568454 92-93 10.635484398701674 94-95 10.762224074391373 96-97 10.32607696053234 98-99 10.168094030820225 100-101 10.274311414169869 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2066046.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 97.52907946359863 #Duplication Level Percentage of deduplicated Percentage of total 1 98.93920427367951 96.49449515672906 2 0.7678679165196618 1.4977890209558804 3 0.15600392821678266 0.45644758535064395 4 0.050090072252911594 0.1954095454836645 5 0.0244514145422626 0.11923619759448602 6 0.015317303706274618 0.08963295182023986 7 0.010251342782938567 0.06998628174200627 8 0.0058713555971325 0.04581023252734241 9 0.004520220261554032 0.03967676289738646 >10 0.023880136217316987 0.38334741657847315 >50 0.001296208418526736 0.08727531479719099 >100 0.0010467116256109108 0.16172770427846628 >500 4.977904487695915E-5 0.03971311818363278 >1k 1.4933713463087742E-4 0.319452711061496 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 3795 0.18368419677006223 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 9.680326575497352E-5 0.0 0.0 0.0 6 0.0 9.680326575497352E-5 0.0 0.0 0.0 7 0.0 9.680326575497352E-5 0.0 0.0 0.0 8 0.0 9.680326575497352E-5 0.0 0.0 0.0 9 0.0 9.680326575497352E-5 0.0 0.0 0.0 10-11 0.0 1.4520489863246026E-4 0.0 0.0 0.0 12-13 0.0 1.4520489863246026E-4 0.0 0.0 9.680326575497352E-5 14-15 0.0 1.4520489863246026E-4 0.0 2.420081643874338E-5 1.9360653150994703E-4 16-17 0.0 1.4520489863246026E-4 0.0 4.840163287748676E-5 2.4200816438743378E-4 18-19 0.0 1.4520489863246026E-4 0.0 9.680326575497352E-5 3.3881143014240727E-4 20-21 0.0 1.4520489863246026E-4 0.0 1.9360653150994703E-4 3.8721306301989407E-4 22-23 0.0 1.4520489863246026E-4 0.0 3.8721306301989407E-4 3.8721306301989407E-4 24-25 0.0 1.4520489863246026E-4 0.0 6.534220438460712E-4 3.8721306301989407E-4 26-27 0.0 1.4520489863246026E-4 0.0 0.0010164342904272219 3.8721306301989407E-4 28-29 0.0 1.4520489863246026E-4 0.0 0.002105471030170674 3.8721306301989407E-4 30-31 0.0 1.4520489863246026E-4 0.0 0.006243810641195792 3.8721306301989407E-4 32-33 0.0 1.4520489863246026E-4 0.0 0.017037374772875338 3.8721306301989407E-4 34-35 0.0 1.4520489863246026E-4 0.0 0.0323564915785999 3.8721306301989407E-4 36-37 0.0 1.4520489863246026E-4 0.0 0.0550084557652637 3.8721306301989407E-4 38-39 0.0 1.4520489863246026E-4 0.0 0.0884297832671683 3.8721306301989407E-4 40-41 4.840163287748676E-5 1.4520489863246026E-4 0.0 0.12294014750881636 4.1141387945863744E-4 42-43 4.840163287748676E-5 1.4520489863246026E-4 0.0 0.15747471256690315 4.356146958973808E-4 44-45 4.840163287748676E-5 1.4520489863246026E-4 0.0 0.2013023911374674 4.356146958973808E-4 46-47 4.840163287748676E-5 1.4520489863246026E-4 0.0 0.2513496795327887 4.356146958973808E-4 48-49 4.840163287748676E-5 1.4520489863246026E-4 0.0 0.30638233611449117 4.356146958973808E-4 50-51 4.840163287748676E-5 1.4520489863246026E-4 2.420081643874338E-5 0.36971587273468254 4.8401632877486756E-4 52-53 4.840163287748676E-5 1.4520489863246026E-4 4.840163287748676E-5 0.4560401849716802 5.80819594529841E-4 54-55 4.840163287748676E-5 1.4520489863246026E-4 4.840163287748676E-5 0.5478096809073951 6.776228602848145E-4 56-57 4.840163287748676E-5 1.4520489863246026E-4 4.840163287748676E-5 0.6482914707610576 7.018236767235579E-4 58-59 4.840163287748676E-5 1.4520489863246026E-4 4.840163287748676E-5 0.7633905537437211 7.502253096010447E-4 60-61 4.840163287748676E-5 1.4520489863246026E-4 4.840163287748676E-5 0.8875891437073521 7.986269424785315E-4 62-63 4.840163287748676E-5 1.4520489863246026E-4 4.840163287748676E-5 1.006342549972266 9.196310246722484E-4 64-65 4.840163287748676E-5 1.4520489863246026E-4 4.840163287748676E-5 1.1312187627961818 9.922334739884786E-4 66-67 4.840163287748676E-5 1.9360653150994703E-4 4.840163287748676E-5 1.2738583748861352 0.0010164342904272219 68-69 4.840163287748676E-5 1.9360653150994703E-4 4.840163287748676E-5 1.4282837845817566 0.0010164342904272219 70-71 4.840163287748676E-5 1.9360653150994703E-4 4.840163287748676E-5 1.5929703404474054 0.0010648359233047086 72-73 4.840163287748676E-5 1.9360653150994703E-4 4.840163287748676E-5 1.7642879200172699 0.0011132375561821953 74-75 4.840163287748676E-5 1.9360653150994703E-4 4.840163287748676E-5 1.9328466065131173 0.001161639189059682 76-77 4.840163287748676E-5 1.9360653150994703E-4 4.840163287748676E-5 2.1244444702586485 0.0012100408219371688 78-79 4.840163287748676E-5 2.4200816438743378E-4 4.840163287748676E-5 2.3374842573689065 0.0012100408219371688 80-81 4.840163287748676E-5 2.4200816438743378E-4 4.840163287748676E-5 2.5600591661560292 0.0012100408219371688 82-83 4.840163287748676E-5 2.4200816438743378E-4 4.840163287748676E-5 2.7914915737597323 0.0012584424548146556 84-85 4.840163287748676E-5 2.4200816438743378E-4 4.840163287748676E-5 3.02880477975805 0.0012584424548146556 86-87 4.840163287748676E-5 2.4200816438743378E-4 4.840163287748676E-5 3.269820710671495 0.0013068440876921423 88-89 4.840163287748676E-5 2.4200816438743378E-4 4.840163287748676E-5 3.5222594269440277 0.001355245720569629 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1700 0.0 54.141834 1 GTATCAA 3355 0.0 49.471172 1 ATCAACG 4135 0.0 39.15107 3 TCAACGC 4200 0.0 38.64872 4 TATCAAC 4345 0.0 37.847126 2 CAACGCA 4305 0.0 37.262836 5 AACGCAG 4695 0.0 34.941216 6 ACGCAGA 5445 0.0 29.313084 7 CGCAGAG 5620 0.0 27.951105 8 GTACATG 4180 0.0 25.388494 1 GCAGAGT 6130 0.0 24.568094 9 TACATGG 4315 0.0 24.357933 2 ACATGGG 4520 0.0 23.247225 3 GTGGTAT 820 0.0 21.467672 1 AGAGTAC 5565 0.0 20.550714 10-11 CAGAGTA 5820 0.0 20.145363 10-11 GAGTACT 3890 0.0 19.997385 12-13 GCGCGTA 55 0.004720425 19.824162 86-87 GTGTAGC 765 0.0 19.7238 1 CATGGGG 3315 0.0 19.70796 4 >>END_MODULE