FastQCFastQC Report
Sat 18 Jun 2016
SRR1930902_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1930902_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2066046
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44840.21703292182265063No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36380.17608514040829681No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24780.11993924627041218No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA27500.054.9203261
GTATCAA50750.039.586131
ATCAACG60150.032.6764683
TCAACGC60300.032.510944
CAACGCA60350.032.3180925
TATCAAC63600.030.9800722
AACGCAG64300.030.774096
ACGCAGA74950.025.8947547
CGCAGAG76600.025.2130728
GTACATG56600.023.4952621
GTGGTAT11350.023.014711
ACATGGG57000.022.7383123
TACATGG58150.022.5346492
GCAGAGT86750.021.7160669
CATGGGG42800.020.7393264
TGGTATC12900.019.8744662
AGAGTAC80800.019.76213510-11
CAGAGTA85050.019.36044310-11
GAGTACT59700.018.99694312-13
TAACGCA2001.2931427E-518.9870194
Produced by FastQC (version 0.11.5)