FastQCFastQC Report
Sat 18 Jun 2016
SRR1930901_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1930901_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2597546
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN46850.18036254218404602No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA19950.057.1453441
GTATCAA43650.049.462361
ATCAACG52200.040.2841953
TCAACGC53350.039.401564
TATCAAC53750.039.0347562
CAACGCA55350.036.1893845
AACGCAG61050.033.7543646
CGCAGAG74200.029.3749758
ACGCAGA71500.029.092677
GTACATG51250.025.6898751
GCAGAGT80350.025.6734319
TACATGG53300.024.2241952
ACATGGG55400.022.8472063
AGAGTAC73800.020.89113410-11
GTGGTAT11250.019.7294731
GAGTACT55050.019.33319312-13
CAGAGTA79300.019.21903810-11
CATGGGG39700.019.1733594
AGTACTT59850.018.70245612-13
GTATAGG7400.018.4054891
Produced by FastQC (version 0.11.5)