##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1930901_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2597546 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 22.468434822713437 30.0 10.0 31.0 2.0 33.0 2 21.417356997720155 28.0 10.0 31.0 2.0 33.0 3 21.84999957652338 28.0 10.0 31.0 2.0 33.0 4 23.766250145329476 32.0 2.0 35.0 2.0 37.0 5 23.75306770313211 32.0 10.0 35.0 2.0 37.0 6 24.469274846335733 32.0 11.0 35.0 2.0 37.0 7 24.53738798080958 32.0 11.0 35.0 2.0 37.0 8 25.268637783507973 32.0 17.0 35.0 2.0 37.0 9 26.365525769322275 33.0 17.0 37.0 2.0 39.0 10-11 26.58298909817189 33.0 17.0 37.0 2.0 39.0 12-13 26.492650370773028 33.0 17.0 37.0 2.0 39.0 14-15 26.680287278839337 33.0 14.0 38.0 2.0 40.0 16-17 25.80341560842426 32.5 10.0 38.0 2.0 40.0 18-19 25.21416694834278 32.0 10.0 38.0 2.0 40.0 20-21 24.70605332879572 32.0 8.0 38.0 2.0 40.0 22-23 24.628309758518235 31.5 10.0 38.0 2.0 40.0 24-25 24.314565940314438 31.0 5.0 37.0 2.0 39.5 26-27 23.94925768398327 30.5 5.0 37.0 2.0 39.0 28-29 23.839933537269406 30.0 2.0 37.0 2.0 39.0 30-31 23.7143942782919 30.0 2.0 37.0 2.0 39.0 32-33 23.37495543871023 30.0 2.0 37.0 2.0 39.0 34-35 20.544408068230553 24.5 2.0 36.0 2.0 39.0 36-37 18.96365107682405 17.0 2.0 34.0 2.0 38.5 38-39 18.048010506839915 16.0 2.0 33.0 2.0 38.0 40-41 19.26106602154495 20.5 2.0 34.5 2.0 38.0 42-43 19.144623040361942 20.5 2.0 34.5 2.0 38.0 44-45 17.267974080151035 13.5 2.0 33.0 2.0 38.0 46-47 18.02451486903408 18.0 2.0 32.5 2.0 38.0 48-49 17.40003949881927 14.5 2.0 33.0 2.0 38.0 50-51 15.577154167818396 10.0 2.0 30.5 2.0 37.0 52-53 18.354025106773854 19.0 2.0 32.5 2.0 37.5 54-55 19.86357046227478 25.0 2.0 35.0 2.0 38.5 56-57 20.04466985377737 26.0 2.0 35.0 2.0 39.0 58-59 19.675793999413294 24.5 2.0 35.0 2.0 39.0 60-61 19.34295523544145 23.5 2.0 35.0 2.0 38.0 62-63 18.857030635838594 22.5 2.0 34.0 2.0 38.0 64-65 18.346575190583728 21.5 2.0 34.0 2.0 38.0 66-67 17.919036659986002 20.0 2.0 34.0 2.0 37.5 68-69 17.462179688059422 18.0 2.0 33.0 2.0 37.0 70-71 16.872308517346756 12.5 2.0 32.5 2.0 36.0 72-73 16.389050665512755 8.0 2.0 32.0 2.0 36.0 74-75 15.852255936949721 7.0 2.0 31.0 2.0 35.0 76-77 15.28731849214605 2.0 2.0 31.0 2.0 35.0 78-79 14.786222072679369 2.0 2.0 31.0 2.0 34.0 80-81 14.269877607557287 2.0 2.0 30.0 2.0 34.0 82-83 13.786934860826335 2.0 2.0 29.5 2.0 34.0 84-85 13.267894389550753 2.0 2.0 29.0 2.0 34.0 86-87 12.717119350340667 2.0 2.0 29.0 2.0 34.0 88-89 12.177486365977735 2.0 2.0 28.5 2.0 34.0 90-91 11.608099336835613 2.0 2.0 26.5 2.0 33.5 92-93 10.995419330398768 2.0 2.0 25.5 2.0 33.0 94-95 10.359101628999063 2.0 2.0 25.0 2.0 32.5 96-97 9.661671439119846 2.0 2.0 21.5 2.0 32.0 98-99 8.900424670053965 2.0 2.0 10.0 2.0 32.0 100-101 7.93307548740234 2.0 2.0 2.0 2.0 31.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 7.132023386320249 1101 2 8.128885152031973 1101 3 7.7142493943881725 1101 4 9.64223854568808 1101 5 9.284001276266494 1101 6 8.819725612698104 1101 7 8.570220002500545 1101 8 7.819707742653922 1101 9 8.069180295479992 1101 10-11 7.618730042484554 1101 12-13 7.213195456972702 1101 14-15 7.816789404437298 1101 16-17 8.464293180033575 1101 18-19 9.014076267786951 1101 20-21 9.42700306665694 1101 22-23 9.357347701474456 1101 24-25 9.34451240219768 1101 26-27 9.45539395552376 1101 28-29 9.461672758831622 1101 30-31 9.234936322986837 1101 32-33 8.989974858292268 1101 34-35 11.434096865153847 1101 36-37 5.707087617695361 1101 38-39 8.841434977667127 1101 40-41 10.335553635611106 1101 42-43 10.710585631174904 1101 44-45 5.042778433092288 1101 46-47 6.83579846010371 1101 48-49 9.7149762169993 1101 50-51 -2.72226466694063 1101 52-53 6.508334819629443 1101 54-55 9.084406125265872 1101 56-57 9.6114630937236 1101 58-59 9.750185813866281 1101 60-61 9.612282370114425 1101 62-63 9.665129577691726 1101 64-65 9.63229045148585 1101 66-67 9.615166692684753 1101 68-69 9.624483777532479 1101 70-71 9.57579010028719 1101 72-73 9.434613044494355 1101 74-75 9.43810365393958 1101 76-77 9.223571430517252 1101 78-79 9.07098768654533 1101 80-81 8.751467359771121 1101 82-83 8.623484378069024 1101 84-85 8.649342039043754 1101 86-87 8.390018034291236 1101 88-89 8.340565899563972 1101 90-91 8.179130426901052 1101 92-93 8.006664291078094 1101 94-95 7.56145190632231 1101 96-97 7.212100953010154 1101 98-99 7.037092031031149 1101 100-101 6.561847991305456 1102 1 6.670031944733722 1102 2 7.631528290730419 1102 3 7.400187675650155 1102 4 9.275097025112608 1102 5 9.292287292923191 1102 6 8.44362291173643 1102 7 8.203022499471356 1102 8 7.4763644398425235 1102 9 7.40494403418764 1102 10-11 7.067346662371367 1102 12-13 6.700857691056047 1102 14-15 7.108490427459127 1102 16-17 7.758124220386957 1102 18-19 7.96484123350729 1102 20-21 8.480142915427638 1102 22-23 8.494110842013423 1102 24-25 8.483296892757352 1102 26-27 8.501627410024597 1102 28-29 8.304424028022868 1102 30-31 8.037936905817858 1102 32-33 8.112909259126635 1102 34-35 10.497217771916223 1102 36-37 1.3075605543968436 1102 38-39 3.5892752052779784 1102 40-41 8.141044057243342 1102 42-43 9.388327007576382 1102 44-45 1.333804369072901 1102 46-47 1.9780073896885213 1102 48-49 7.400597392170699 1102 50-51 -4.2815534597135265 1102 52-53 4.946344835120474 1102 54-55 7.754726375576457 1102 56-57 8.328013500938432 1102 58-59 8.578755960647673 1102 60-61 8.577472020262434 1102 62-63 8.256493862420019 1102 64-65 8.340443950772652 1102 66-67 8.441485347878853 1102 68-69 8.21763658662464 1102 70-71 8.053639530493175 1102 72-73 8.069718184143738 1102 74-75 7.817366756134399 1102 76-77 7.803975424443534 1102 78-79 7.596925891119021 1102 80-81 7.553191005839311 1102 82-83 7.421409652996557 1102 84-85 7.359535830359571 1102 86-87 7.283187561431218 1102 88-89 7.111486772583499 1102 90-91 7.08132769986536 1102 92-93 6.948857192809717 1102 94-95 6.826602598817596 1102 96-97 6.433671529245986 1102 98-99 6.255988409809964 1102 100-101 5.741489090095314 1103 1 6.604560149611736 1103 2 7.591060379854156 1103 3 7.339648384722029 1103 4 9.054402964818607 1103 5 9.014339075360397 1103 6 8.218863115743769 1103 7 7.866977975131956 1103 8 7.248875088680439 1103 9 7.44141301088592 1103 10-11 6.960005550538863 1103 12-13 6.810386815393198 1103 14-15 7.124078599837073 1103 16-17 7.692390923581794 1103 18-19 8.170489714898554 1103 20-21 8.4467119067622 1103 22-23 8.247429413962042 1103 24-25 8.363132816213316 1103 26-27 8.379125430372795 1103 28-29 8.219829809732012 1103 30-31 8.004939806805243 1103 32-33 7.972540499720946 1103 34-35 10.211669868095402 1103 36-37 -2.9240063165523775 1103 38-39 -1.5426421860100525 1103 40-41 6.420383697941226 1103 42-43 8.624943848314803 1103 44-45 1.0326387200661067 1103 46-47 0.8910778953159877 1103 48-49 7.2628955896555105 1103 50-51 -4.638556998692964 1103 52-53 4.650927644612295 1103 54-55 7.564863919156945 1103 56-57 8.037874681373843 1103 58-59 8.167161093106081 1103 60-61 7.9945064080340025 1103 62-63 8.151681000500265 1103 64-65 7.988851785315042 1103 66-67 7.695985631222378 1103 68-69 7.790369256696433 1103 70-71 7.898759379176468 1103 72-73 7.580856474550213 1103 74-75 7.250633538140024 1103 76-77 7.4648333361079064 1103 78-79 7.200202618294853 1103 80-81 6.87052967829044 1103 82-83 6.842955320092303 1103 84-85 6.738870141947386 1103 86-87 6.503695853451438 1103 88-89 6.586483939148211 1103 90-91 6.497076946471806 1103 92-93 6.4186173453273465 1103 94-95 6.171698729208158 1103 96-97 5.880136415289346 1103 98-99 5.449405832997597 1103 100-101 5.031006580459756 1104 1 6.402401731170698 1104 2 7.449943710791249 1104 3 7.025871760111428 1104 4 8.650595807412692 1104 5 8.768645220021853 1104 6 7.9343430525295595 1104 7 7.755324007082432 1104 8 7.030480776738283 1104 9 7.104611841268923 1104 10-11 6.5454060101807805 1104 12-13 6.270794852628587 1104 14-15 6.775578134714575 1104 16-17 7.433053038798146 1104 18-19 7.713840236709455 1104 20-21 8.197366632643753 1104 22-23 8.179837062177949 1104 24-25 7.918403601243277 1104 26-27 8.069552062332022 1104 28-29 7.997761109997622 1104 30-31 7.527013827209576 1104 32-33 7.393677268123373 1104 34-35 9.844157602570125 1104 36-37 -3.0265667363212536 1104 38-39 -1.8800335720198582 1104 40-41 5.8964263638992485 1104 42-43 8.44808419784793 1104 44-45 0.5614054454685089 1104 46-47 0.4077906865699674 1104 48-49 6.7570690199452095 1104 50-51 -4.764057065346712 1104 52-53 3.7341560788539745 1104 54-55 6.4148545352977955 1104 56-57 7.04477774182029 1104 58-59 7.055600091407101 1104 60-61 7.102509902231343 1104 62-63 7.240677346246702 1104 64-65 7.176103024006256 1104 66-67 7.199005329476428 1104 68-69 7.138139001942466 1104 70-71 7.095864178784197 1104 72-73 6.836060155174529 1104 74-75 6.941108070666106 1104 76-77 6.8015218412656875 1104 78-79 6.564672507300735 1104 80-81 6.116254570870327 1104 82-83 6.045054062713325 1104 84-85 5.865502377386159 1104 86-87 5.462459716449713 1104 88-89 5.408487031823961 1104 90-91 5.316680504226445 1104 92-93 5.279766031448959 1104 94-95 4.637939877961152 1104 96-97 4.262942917496918 1104 98-99 4.313753026773055 1104 100-101 4.1088724045028755 1105 1 5.895226137490148 1105 2 6.9683148676893545 1105 3 6.6852251318672415 1105 4 8.439720213048865 1105 5 8.236968150114386 1105 6 7.528552517087217 1105 7 7.256960316031286 1105 8 6.578242326455122 1105 9 6.551159570328121 1105 10-11 6.060012659469901 1105 12-13 6.124319718263553 1105 14-15 6.391639773550249 1105 16-17 6.77455146245023 1105 18-19 7.487908215518946 1105 20-21 7.75605768588456 1105 22-23 7.77111268872552 1105 24-25 7.727720794179646 1105 26-27 7.640444002354805 1105 28-29 7.419053875642124 1105 30-31 7.1594810163182 1105 32-33 7.253301496456139 1105 34-35 9.20801013019997 1105 36-37 -3.4462012305421936 1105 38-39 -2.141072574167719 1105 40-41 5.352745372260745 1105 42-43 7.525689125139589 1105 44-45 -3.211650307284504 1105 46-47 -4.054441177759667 1105 48-49 4.777416799566904 1105 50-51 -9.221280160722559 1105 52-53 0.483552808020967 1105 54-55 5.072108364193799 1105 56-57 6.320910751029331 1105 58-59 6.571707928134021 1105 60-61 6.347707197068015 1105 62-63 6.392280334983926 1105 64-65 6.3007266813146785 1105 66-67 6.186815110182167 1105 68-69 6.116674064440698 1105 70-71 5.97014107301975 1105 72-73 5.972616478184943 1105 74-75 5.9748275809991345 1105 76-77 5.629182720576241 1105 78-79 5.563140851286917 1105 80-81 5.325639645284072 1105 82-83 5.197942658025516 1105 84-85 5.239563621036739 1105 86-87 4.9271902829016785 1105 88-89 4.6991383523682995 1105 90-91 4.6313302557437 1105 92-93 4.702128753083448 1105 94-95 4.100986794129897 1105 96-97 3.979666107904041 1105 98-99 3.809604977661335 1105 100-101 3.4756929339923452 1106 1 5.700339393864017 1106 2 6.78055475369921 1106 3 6.359256854298543 1106 4 7.659105934877033 1106 5 7.6901001117775785 1106 6 6.911582969981943 1106 7 6.755315968295825 1106 8 5.9484144119995115 1106 9 6.001788123686499 1106 10-11 5.724144612018737 1106 12-13 5.6065585567835505 1106 14-15 5.716249946829901 1106 16-17 6.446104477787838 1106 18-19 6.671970082674246 1106 20-21 7.376035431017993 1106 22-23 6.826071232299842 1106 24-25 7.064980893573619 1106 26-27 6.948613274074596 1106 28-29 6.913315599371888 1106 30-31 6.334685187679089 1106 32-33 6.450604203457903 1106 34-35 8.755403168341939 1106 36-37 -0.02650698134078766 1106 38-39 1.1378232526353145 1106 40-41 6.405299576558782 1106 42-43 7.496557679163711 1106 44-45 -3.365364491023735 1106 46-47 -4.217981556116175 1106 48-49 4.103589357472423 1106 50-51 -9.204181771043384 1106 52-53 0.0852632810306666 1106 54-55 4.31889902301473 1106 56-57 5.356612322884956 1106 58-59 5.833041259882247 1106 60-61 5.823386014749172 1106 62-63 5.725351399575747 1106 64-65 5.71320276109774 1106 66-67 5.6872696388850414 1106 68-69 5.339563461531686 1106 70-71 5.391289787651942 1106 72-73 5.207736937537646 1106 74-75 5.207812970695086 1106 76-77 4.925823923860735 1106 78-79 5.088762942042337 1106 80-81 4.885265396838237 1106 82-83 4.7082213339190755 1106 84-85 4.474616713450045 1106 86-87 4.289883581436685 1106 88-89 4.166875070216667 1106 90-91 3.8656777124898323 1106 92-93 3.66862109788752 1106 94-95 3.5438326166993814 1106 96-97 3.048594969604867 1106 98-99 2.8017087137657466 1106 100-101 2.561337250554084 1107 1 5.8277380557363685 1107 2 7.080325436680969 1107 3 6.329547111939892 1107 4 7.662126719296243 1107 5 7.875092709937441 1107 6 6.43166146270287 1107 7 6.701512764109896 1107 8 6.134548710622241 1107 9 6.040508898141066 1107 10-11 5.630703365305283 1107 12-13 5.551750291471379 1107 14-15 5.7935073778149935 1107 16-17 6.388278814732104 1107 18-19 6.761673025630252 1107 20-21 7.29191125464774 1107 22-23 6.981241350879081 1107 24-25 7.241056960913756 1107 26-27 7.2080862943184485 1107 28-29 7.133941430979863 1107 30-31 6.912581658307165 1107 32-33 6.551195764513476 1107 34-35 8.654987779548318 1107 36-37 -0.22638701486888024 1107 38-39 0.9292737853528799 1107 40-41 6.615945461194279 1107 42-43 7.493459326846544 1107 44-45 -3.2665151140743625 1107 46-47 -4.11205526450647 1107 48-49 4.041806103411179 1107 50-51 -9.158119725842989 1107 52-53 0.12839591692591057 1107 54-55 4.392634115943157 1107 56-57 5.598511362176254 1107 58-59 6.054095766472916 1107 60-61 5.978961166709954 1107 62-63 5.851187957306518 1107 64-65 5.844556408840489 1107 66-67 5.686846630071216 1107 68-69 5.664544167249762 1107 70-71 5.732737878055339 1107 72-73 5.444500669367642 1107 74-75 5.373606439491107 1107 76-77 5.2528574109187485 1107 78-79 5.257629818986825 1107 80-81 5.05352078344354 1107 82-83 4.882414604985337 1107 84-85 4.85076246815443 1107 86-87 4.483394515514393 1107 88-89 4.255387661811591 1107 90-91 4.169594858772728 1107 92-93 4.032864576420231 1107 94-95 3.797967324328324 1107 96-97 3.6920123467678625 1107 98-99 3.232080420956377 1107 100-101 3.04255793871355 1108 1 6.207607213226005 1108 2 6.466762157197056 1108 3 6.720950439032112 1108 4 8.225239118943598 1108 5 8.055862355224775 1108 6 7.493093179393288 1108 7 7.833738941046509 1108 8 6.683726443162907 1108 9 5.126470271953956 1108 10-11 5.878408935517161 1108 12-13 5.523843403462688 1108 14-15 6.147577144867665 1108 16-17 6.447733702315052 1108 18-19 7.011953674982902 1108 20-21 7.756298809815338 1108 22-23 7.832762212648316 1108 24-25 7.496757874722775 1108 26-27 7.969454551119469 1108 28-29 7.721491711896977 1108 30-31 7.403288265432209 1108 32-33 7.529334836727216 1108 34-35 9.766803435263693 1108 36-37 -3.096256888625202 1108 38-39 -1.9008433543733432 1108 40-41 5.551505959956881 1108 42-43 8.027346037555802 1108 44-45 -2.8492794616658657 1108 46-47 -3.6951986979140834 1108 48-49 5.175020929194371 1108 50-51 -9.116187666750317 1108 52-53 0.9880235118027976 1108 54-55 5.717646817962159 1108 56-57 6.715908542763152 1108 58-59 7.260613525535529 1108 60-61 7.199509184358231 1108 62-63 7.289829139159931 1108 64-65 6.922137060803827 1108 66-67 6.9138760579214065 1108 68-69 6.999244945168936 1108 70-71 6.869357447398322 1108 72-73 6.764003004597853 1108 74-75 6.48587468203373 1108 76-77 6.480581952156632 1108 78-79 6.3372406518844855 1108 80-81 6.283662222684379 1108 82-83 6.141476416122549 1108 84-85 6.03800241146895 1108 86-87 5.982716806737715 1108 88-89 5.628377914560014 1108 90-91 5.6280369318761085 1108 92-93 5.717624563487268 1108 94-95 5.514968750837021 1108 96-97 5.301873866310899 1108 98-99 4.965187003447204 1108 100-101 4.672013703221129 1109 1 4.8026273523691785 1109 2 6.37961459220255 1109 3 6.136549120833649 1109 4 7.493272806237627 1109 5 7.525653640468278 1109 6 5.037216211249749 1109 7 4.662006974733803 1109 8 3.091269467696044 1109 9 2.886997551338794 1109 10-11 2.666215599742717 1109 12-13 3.401177713239324 1109 14-15 3.8388990049921574 1109 16-17 2.7954085466944 1109 18-19 3.6459705186299196 1109 20-21 4.141505693740637 1109 22-23 5.732249579913052 1109 24-25 5.585736270914644 1109 26-27 4.466708304323426 1109 28-29 4.39596992500573 1109 30-31 4.266342091869415 1109 32-33 3.9011766529117615 1109 34-35 6.2852948303570635 1109 36-37 -4.415004600086206 1109 38-39 -3.176566532696304 1109 40-41 2.964907644321581 1109 42-43 3.670150870950163 1109 44-45 -4.420132629003836 1109 46-47 -5.700141942146965 1109 48-49 1.8007990324532521 1109 50-51 -9.510731789782174 1109 52-53 -1.1463594980836938 1109 54-55 2.076306649525687 1109 56-57 3.7129792128473724 1109 58-59 4.464018871562626 1109 60-61 4.56329534327438 1109 62-63 4.539005265121119 1109 64-65 4.209760641909654 1109 66-67 4.402524904497753 1109 68-69 4.366143517120602 1109 70-71 4.571481211587265 1109 72-73 4.292930137516201 1109 74-75 4.5574601818506455 1109 76-77 4.6660451524495645 1109 78-79 4.690041823616454 1109 80-81 4.5460203332665845 1109 82-83 4.347444925825952 1109 84-85 4.254040492757854 1109 86-87 4.208275210252912 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2313 72-73 -14.124108385541424 2313 74-75 -13.570929468010041 2313 76-77 -13.061087659412996 2313 78-79 -12.562860453122969 2313 80-81 -12.002482517998446 2313 82-83 -11.536702525099319 2313 84-85 -11.030702141357684 2313 86-87 -10.473020137093236 2313 88-89 -9.95237152431119 2313 90-91 -9.377668393346994 2313 92-93 -8.79222379098082 2313 94-95 -8.234756624483376 2313 96-97 -7.547583160134398 2313 98-99 -6.751546008389243 2313 100-101 -5.795927983307826 2314 1 -20.27792922031482 2314 2 -19.259345495677348 2314 3 -19.651074460272174 2314 4 -21.552864139935934 2314 5 -21.54538260998943 2314 6 -22.260843107681044 2314 7 -22.325909539363597 2314 8 -23.066548181516698 2314 9 -24.149619072608477 2314 10-11 -24.365274696851937 2314 12-13 -24.269821920594435 2314 14-15 -24.447626077395245 2314 16-17 -23.579545682525666 2314 18-19 -22.992440319890772 2314 20-21 -22.51869203602868 2314 22-23 -22.394627022064245 2314 24-25 -22.088741942194105 2314 26-27 -21.741091036577345 2314 28-29 -21.641012865181015 2314 30-31 -21.492354356318064 2314 32-33 -21.148255789417053 2314 34-35 -18.437151160122614 2314 36-37 -16.82273070773908 2314 38-39 -15.933249066566681 2314 40-41 -17.137985068970252 2314 42-43 -17.017692409899343 2314 44-45 -15.134156795975642 2314 46-47 -15.845876105457108 2314 48-49 -15.28561620006231 2314 50-51 -13.412233071364012 2314 52-53 -16.157344485267874 2314 54-55 -17.634788187530337 2314 56-57 -17.804413683527585 2314 58-59 -17.445904233527084 2314 60-61 -17.10019788265019 2314 62-63 -16.63107895311396 2314 64-65 -16.12363371913026 2314 66-67 -15.69249523464542 2314 68-69 -15.227411957064678 2314 70-71 -14.66927309086605 2314 72-73 -14.195718709060145 2314 74-75 -13.652536156486958 2314 76-77 -13.101073119245784 2314 78-79 -12.596666341890689 2314 80-81 -12.07554685823836 2314 82-83 -11.617036948945193 2314 84-85 -11.112308829834602 2314 86-87 -10.553899817209556 2314 88-89 -10.028707402173785 2314 90-91 -9.456730552561824 2314 92-93 -8.862016593598051 2314 94-95 -8.234756624483376 2314 96-97 -7.56594012123945 2314 98-99 -6.796438774656055 2314 100-101 -5.843365278836725 2315 1 -20.27792922031482 2315 2 -19.259345495677348 2315 3 -19.651074460272174 2315 4 -17.08436305376649 2315 5 -21.54538260998943 2315 6 -22.260843107681044 2315 7 -22.325909539363597 2315 8 -23.066548181516698 2315 9 -20.01058938397705 2315 10-11 -18.57092277795259 2315 12-13 -15.507330972006454 2315 14-15 -15.477495737062153 2315 16-17 -20.09945878897027 2315 18-19 -22.992440319890772 2315 20-21 -22.51869203602868 2315 22-23 -22.394627022064245 2315 24-25 -22.088741942194105 2315 26-27 -21.741091036577345 2315 28-29 -21.641012865181015 2315 30-31 -21.492354356318064 2315 32-33 -21.148255789417053 2315 34-35 -18.437151160122614 2315 36-37 -16.82273070773908 2315 38-39 -15.933249066566681 2315 40-41 -17.137985068970252 2315 42-43 -17.017692409899343 2315 44-45 -15.134156795975642 2315 46-47 -15.845876105457108 2315 48-49 -15.28561620006231 2315 50-51 -13.412233071364012 2315 52-53 -16.157344485267874 2315 54-55 -17.634788187530337 2315 56-57 -17.804413683527585 2315 58-59 -17.445904233527084 2315 60-61 -17.10019788265019 2315 62-63 -16.63107895311396 2315 64-65 -16.12363371913026 2315 66-67 -15.69249523464542 2315 68-69 -15.227411957064678 2315 70-71 -14.66927309086605 2315 72-73 -14.195718709060145 2315 74-75 -13.652536156486958 2315 76-77 -13.101073119245784 2315 78-79 -12.596666341890689 2315 80-81 -12.07554685823836 2315 82-83 -11.617036948945193 2315 84-85 -11.112308829834602 2315 86-87 -10.553899817209556 2315 88-89 -10.028707402173785 2315 90-91 -9.456730552561824 2315 92-93 -8.862016593598051 2315 94-95 -8.234756624483376 2315 96-97 -7.56594012123945 2315 98-99 -6.796438774656055 2315 100-101 -5.843365278836725 2316 1 -20.27792922031482 2316 2 -19.259345495677348 2316 3 -19.651074460272174 2316 4 -21.552864139935934 2316 5 -21.54538260998943 2316 6 -22.260843107681044 2316 7 -22.325909539363597 2316 8 -23.066548181516698 2316 9 -24.149619072608477 2316 10-11 -24.365274696851937 2316 12-13 -24.269821920594435 2316 14-15 -24.447626077395245 2316 16-17 -23.579545682525666 2316 18-19 -22.992440319890772 2316 20-21 -22.51869203602868 2316 22-23 -22.394627022064245 2316 24-25 -22.088741942194105 2316 26-27 -21.741091036577345 2316 28-29 -21.641012865181015 2316 30-31 -21.492354356318064 2316 32-33 -21.148255789417053 2316 34-35 -18.437151160122614 2316 36-37 -16.82273070773908 2316 38-39 -15.933249066566681 2316 40-41 -17.137985068970252 2316 42-43 -17.017692409899343 2316 44-45 -15.134156795975642 2316 46-47 -15.845876105457108 2316 48-49 -15.28561620006231 2316 50-51 -13.412233071364012 2316 52-53 -16.157344485267874 2316 54-55 -17.634788187530337 2316 56-57 -17.804413683527585 2316 58-59 -17.445904233527084 2316 60-61 -17.10019788265019 2316 62-63 -16.63107895311396 2316 64-65 -16.12363371913026 2316 66-67 -15.69249523464542 2316 68-69 -15.227411957064678 2316 70-71 -14.66927309086605 2316 72-73 -14.195718709060145 2316 74-75 -13.652536156486958 2316 76-77 -13.101073119245784 2316 78-79 -12.596666341890689 2316 80-81 -12.07554685823836 2316 82-83 -11.617036948945193 2316 84-85 -11.112308829834602 2316 86-87 -10.553899817209556 2316 88-89 -10.028707402173785 2316 90-91 -9.456730552561824 2316 92-93 -8.862016593598051 2316 94-95 -8.234756624483376 2316 96-97 -7.56594012123945 2316 98-99 -6.796438774656055 2316 100-101 -5.843365278836725 >>END_MODULE >>Per sequence quality scores fail #Quality Count 2 453122.0 3 28843.0 4 56983.0 5 37045.0 6 33076.0 7 40900.0 8 51406.0 9 60231.0 10 61318.0 11 56630.0 12 50343.0 13 46218.0 14 44567.0 15 47119.0 16 50828.0 17 53199.0 18 54894.0 19 56788.0 20 59201.0 21 62208.0 22 65311.0 23 69048.0 24 72553.0 25 75970.0 26 79460.0 27 84192.0 28 88318.0 29 92108.0 30 94434.0 31 93352.0 32 90484.0 33 86630.0 34 78876.0 35 64038.0 36 41274.0 37 15125.0 38 1449.0 39 5.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.45181426959688 20.127414353368778 13.898523407714672 23.522247969319668 2 15.399073431396323 20.74505422654887 40.54454313138562 23.311329210669186 3 18.678885379958498 25.819830314271975 30.21719829057203 25.284086015197506 4 12.526133201250017 16.907559581709645 38.90500146206682 31.66130575497351 5 13.150859274716744 37.74018344033524 35.55067343192664 13.558283853021386 6 30.553958918660417 37.70925778609562 18.078180539014397 13.658602756229566 7 26.577004001815258 32.82508571918201 22.974962246384333 17.6229480326184 8 25.09121232862906 35.83340855849026 20.975811971579787 18.09956714130089 9 25.90175599942977 16.25847155261863 21.28598833887084 36.55378410908076 10-11 24.584164537424883 26.416905198041114 29.015740393285338 19.983189871248666 12-13 25.084582126264987 24.151827986097928 29.116295208040988 21.6472946795961 14-15 22.294799345932244 25.58103012135532 26.876456202483933 25.247714330228497 16-17 21.914509947674524 28.53488662668064 27.54064160204165 22.00996182360319 18-19 21.8591677025079 27.514590133978828 29.560547646064894 21.06569451744838 20-21 22.430059057009867 26.758994997289214 29.706360459908502 21.10458548579241 22-23 22.162978686490113 26.6384793919834 29.407872490718823 21.79066943080766 24-25 22.044577874029876 26.855817391357878 29.412491590037497 21.687113144574752 26-27 21.990838036631022 26.96928399296133 29.316887625325922 21.722990345081723 28-29 21.935869666325594 27.214153145611025 29.261070433515386 21.588906754547992 30-31 22.175504414754517 26.975958584316967 29.198803644361792 21.649733356566724 32-33 22.04435521794812 27.131620606515543 29.023960185605958 21.800063989930376 34-35 22.433677716662416 27.01414857880736 29.020083447005046 21.53209025752518 36-37 22.8753557038346 27.007180045081352 28.57213763665016 21.545326614433883 38-39 22.679000318066954 27.102660492327573 28.6222691948398 21.59606999476567 40-41 22.636134045470296 27.00653409518931 28.765862059873744 21.59146979946665 42-43 22.65864971379563 27.066013542662965 28.414140335409787 21.86119640813162 44-45 22.905638788122644 27.30604366933025 28.103206986086924 21.68511055646018 46-47 22.323683862327215 27.188393862408944 28.432404038565483 22.055518236698354 48-49 22.873255861648374 27.357535682241814 27.85912935108929 21.91007910502053 50-51 23.023564464367333 27.527234420102996 27.751831311578385 21.697369803951283 52-53 22.098091342136957 27.623650430234704 28.19787541097451 22.080382816653827 54-55 21.93127766938778 27.527598918924895 28.37543593966761 22.16568747201972 56-57 22.026247607783045 27.718322430723262 28.127151071592277 22.12827888990141 58-59 22.016030182836626 27.605260640628927 28.26477608686464 22.113933089669807 60-61 22.02516559539773 27.64010843269941 28.266188180535273 22.068537791367586 62-63 21.993842492631067 27.63820216332081 28.217867371769945 22.150087972278186 64-65 21.834984203879085 27.488805863348347 28.24605365314366 22.430156279628907 66-67 21.853840985953045 27.6059353994471 28.12714879106384 22.413074823536018 68-69 21.96104818976036 27.552043085873922 28.093989806430482 22.39291891793524 70-71 21.8800507058102 27.388279479522854 28.03826436562698 22.69340544903996 72-73 22.00151009250943 27.57360833612699 27.964076972216812 22.460804599146766 74-75 22.013088067719355 27.74826631938792 27.78950567041076 22.449139942481956 76-77 22.053031927012306 27.569113366836923 27.82717743457559 22.550677271575186 78-79 21.93392855545278 27.649658169509784 27.747545347009606 22.66886792802783 80-81 22.038246568769118 27.686109853917085 27.656162583361056 22.619480993952745 82-83 22.235866228018434 27.60504585601396 27.67951710372052 22.479570812247086 84-85 22.165299229246962 27.67929457198909 27.552921242289685 22.602484956474264 86-87 21.989430710768175 27.772968439774452 27.534080013459473 22.703520835997903 88-89 22.103208210534948 27.537053205027007 27.470657036423912 22.889081548014133 90-91 22.16170540407272 27.502349937864974 27.455520774637886 22.880423883424427 92-93 22.17870882286811 27.402253672229044 27.516279400544263 22.902758104358583 94-95 22.006391477219804 27.34020709879954 27.519523663642158 23.133877760338493 96-97 22.113086964701388 27.19666745072984 27.429791246960676 23.260454337608095 98-99 21.949258789489143 27.285336180897367 27.278608293329 23.48679673628449 100-101 22.369627058113966 27.360843498071674 27.159419564047028 23.110109879767332 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 31557.0 1 33917.0 2 25367.0 3 15440.5 4 19926.5 5 24819.5 6 26241.5 7 25372.0 8 23421.0 9 21374.0 10 19102.5 11 16484.5 12 14127.0 13 12181.5 14 10420.0 15 8987.0 16 8063.5 17 7621.5 18 7591.5 19 7910.5 20 8473.0 21 9302.0 22 10731.0 23 12688.5 24 14762.5 25 17358.0 26 20654.0 27 24455.5 28 28668.0 29 33439.0 30 38563.5 31 43692.0 32 49037.0 33 53637.5 34 57305.0 35 61326.0 36 65681.0 37 69952.0 38 73280.0 39 75082.5 40 76230.5 41 77308.0 42 78259.5 43 79538.5 44 81060.5 45 81766.0 46 81846.0 47 81433.0 48 80242.5 49 78425.5 50 75831.5 51 72462.5 52 68954.5 53 65317.5 54 60828.0 55 55422.0 56 49379.5 57 43287.0 58 37464.0 59 32053.5 60 26987.0 61 22151.5 62 18029.0 63 14407.0 64 11069.5 65 8413.5 66 6525.5 67 4985.5 68 3750.0 69 2896.0 70 2229.0 71 1730.5 72 1342.0 73 1024.5 74 772.0 75 584.5 76 436.5 77 303.5 78 201.0 79 147.0 80 94.5 81 44.5 82 26.0 83 17.5 84 11.5 85 7.0 86 5.0 87 2.5 88 1.0 89 1.5 90 2.0 91 1.0 92 0.5 93 0.5 94 0.0 95 0.0 96 0.5 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 14.522591707711818 2 21.572360989949747 3 17.31222469207475 4 22.586472000880832 5 22.4419509798864 6 16.278287275759507 7 15.083351748149983 8 12.14638739795176 9 3.0515340248064904 10-11 6.538594504197423 12-13 3.581361023057917 14-15 7.461638792922242 16-17 11.571575633309285 18-19 14.673503375878619 20-21 15.073534790144235 22-23 13.017440307120646 24-25 15.570946578039427 26-27 13.078998408497867 28-29 13.08758343451858 30-31 13.946143783401718 32-33 12.403553199827838 34-35 19.324566340692332 36-37 23.98862233816071 38-39 23.14149585801368 40-41 18.232978357264894 42-43 18.16972634940825 44-45 23.457082184492595 46-47 12.859040802357303 48-49 21.62943408894395 50-51 21.246245494786233 52-53 11.301859524335661 54-55 12.931763287348904 56-57 12.444187706396729 58-59 13.470829775488093 60-61 12.880445620597285 62-63 12.838290447984365 64-65 13.919060528668211 66-67 12.211294814413296 68-69 13.26471215524191 70-71 11.867431799090372 72-73 11.230869443698014 74-75 11.315333780421982 76-77 11.37244537729072 78-79 11.785450575273739 80-81 11.748916092342542 82-83 10.257566179771214 84-85 10.16282291054711 86-87 10.301569250361688 88-89 11.722294812103423 90-91 9.199952570618576 92-93 11.221649202747516 94-95 11.342840511775345 96-97 10.882636919615669 98-99 10.734708836725124 100-101 10.82895163358031 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2597546.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 97.75683806110415 #Duplication Level Percentage of deduplicated Percentage of total 1 99.1910900396163 96.9660732610717 2 0.5737982178215517 1.121853989186632 3 0.11150026345793917 0.3269973959588462 4 0.03711762647522426 0.145140072022042 5 0.025007033195362315 0.12223042472338447 6 0.01416116337619116 0.08306103329538948 7 0.00960379599205482 0.06571857106970289 8 0.007982835378485247 0.06243013962904283 9 0.00525824345126037 0.04626263281956493 >10 0.021588823024402185 0.3769527636213489 >50 0.0016640142239608391 0.11176347209667353 >100 0.0010699325521093639 0.1842289021188004 >500 0.0 0.0 >1k 1.5801143507560424E-4 0.3872873423868233 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 4685 0.18036254218404602 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 3.8497874532347067E-5 2 0.0 0.0 0.0 3.8497874532347067E-5 3.8497874532347067E-5 3 0.0 0.0 0.0 3.8497874532347067E-5 3.8497874532347067E-5 4 0.0 0.0 0.0 7.699574906469413E-5 3.8497874532347067E-5 5 0.0 0.0 0.0 7.699574906469413E-5 3.8497874532347067E-5 6 0.0 0.0 0.0 7.699574906469413E-5 3.8497874532347067E-5 7 0.0 0.0 0.0 7.699574906469413E-5 3.8497874532347067E-5 8 0.0 0.0 0.0 7.699574906469413E-5 3.8497874532347067E-5 9 0.0 0.0 0.0 7.699574906469413E-5 3.8497874532347067E-5 10-11 0.0 0.0 0.0 7.699574906469413E-5 3.8497874532347067E-5 12-13 0.0 0.0 0.0 9.624468633086768E-5 1.154936235970412E-4 14-15 0.0 0.0 0.0 1.154936235970412E-4 1.9248937266173535E-4 16-17 0.0 0.0 0.0 1.5399149812938827E-4 3.0798299625877653E-4 18-19 0.0 1.9248937266173533E-5 0.0 1.9248937266173535E-4 3.849787453234707E-4 20-21 0.0 3.8497874532347067E-5 0.0 2.309872471940824E-4 4.4272555712199127E-4 22-23 0.0 5.77468117985206E-5 0.0 2.309872471940824E-4 4.812234316543384E-4 24-25 0.0 7.699574906469413E-5 0.0 4.4272555712199127E-4 5.004723689205119E-4 26-27 0.0 7.699574906469413E-5 0.0 5.967170552513795E-4 5.774681179852061E-4 28-29 0.0 1.154936235970412E-4 0.0 0.001482168169495362 5.774681179852061E-4 30-31 0.0 1.154936235970412E-4 0.0 0.004485002383018433 5.774681179852061E-4 32-33 0.0 1.154936235970412E-4 0.0 0.011202881488912996 5.774681179852061E-4 34-35 0.0 1.154936235970412E-4 0.0 0.021019839494661498 5.774681179852061E-4 36-37 0.0 1.3474256086321474E-4 0.0 0.03561053394242104 5.774681179852061E-4 38-39 0.0 1.5399149812938827E-4 0.0 0.05872850759909545 5.774681179852061E-4 40-41 0.0 1.5399149812938827E-4 0.0 0.08296291961720793 5.774681179852061E-4 42-43 0.0 1.5399149812938827E-4 0.0 0.10796728912596736 5.774681179852061E-4 44-45 0.0 1.5399149812938827E-4 0.0 0.1391313185599023 5.967170552513795E-4 46-47 1.9248937266173533E-5 1.5399149812938827E-4 0.0 0.17782168246491112 6.159659925175531E-4 48-49 3.8497874532347067E-5 1.5399149812938827E-4 0.0 0.2194956316461768 6.544638670499002E-4 50-51 3.8497874532347067E-5 1.5399149812938827E-4 0.0 0.26544284490053305 6.929617415822473E-4 52-53 3.8497874532347067E-5 1.5399149812938827E-4 1.9248937266173533E-5 0.32621173984984286 7.892064279131149E-4 54-55 3.8497874532347067E-5 1.5399149812938827E-4 3.8497874532347067E-5 0.3951614331372765 8.662021769778091E-4 56-57 3.8497874532347067E-5 1.5399149812938827E-4 3.8497874532347067E-5 0.47252291201002794 8.854511142439825E-4 58-59 3.8497874532347067E-5 1.732404353955618E-4 3.8497874532347067E-5 0.558719653087953 8.854511142439825E-4 60-61 3.8497874532347067E-5 1.9248937266173535E-4 3.8497874532347067E-5 0.6505370838476008 8.854511142439825E-4 62-63 3.8497874532347067E-5 1.9248937266173535E-4 3.8497874532347067E-5 0.7424122614190471 0.0010009447378410239 64-65 3.8497874532347067E-5 1.9248937266173535E-4 3.8497874532347067E-5 0.8400043733585469 0.0010201936751071973 66-67 3.8497874532347067E-5 1.9248937266173535E-4 3.8497874532347067E-5 0.9518984456868136 0.0010394426123733708 68-69 7.699574906469413E-5 1.9248937266173535E-4 3.8497874532347067E-5 1.0738789611425554 0.0010394426123733708 70-71 7.699574906469413E-5 1.9248937266173535E-4 3.8497874532347067E-5 1.2054261984195853 0.0010586915496395444 72-73 7.699574906469413E-5 1.9248937266173535E-4 3.8497874532347067E-5 1.336184229268702 0.0010779404869057179 74-75 7.699574906469413E-5 1.9248937266173535E-4 3.8497874532347067E-5 1.4740258690317707 0.0010779404869057179 76-77 7.699574906469413E-5 2.309872471940824E-4 3.8497874532347067E-5 1.6278248777884974 0.0010971894241718913 78-79 7.699574906469413E-5 2.309872471940824E-4 3.8497874532347067E-5 1.8000836173834842 0.0011741851732365856 80-81 7.699574906469413E-5 2.309872471940824E-4 3.8497874532347067E-5 1.9843344448952973 0.0011934341105027592 82-83 7.699574906469413E-5 2.6948512172642947E-4 3.8497874532347067E-5 2.171761347056029 0.0011934341105027592 84-85 7.699574906469413E-5 2.6948512172642947E-4 3.8497874532347067E-5 2.359515481150286 0.0011934341105027592 86-87 7.699574906469413E-5 2.8873405899260303E-4 3.8497874532347067E-5 2.56116734795072 0.0012319319850351063 88-89 7.699574906469413E-5 3.4648087079112365E-4 3.8497874532347067E-5 2.776177977213878 0.0012704298595674532 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1995 0.0 57.145344 1 GTATCAA 4365 0.0 49.46236 1 ATCAACG 5220 0.0 40.284195 3 TCAACGC 5335 0.0 39.40156 4 TATCAAC 5375 0.0 39.034756 2 CAACGCA 5535 0.0 36.189384 5 AACGCAG 6105 0.0 33.754364 6 CGCAGAG 7420 0.0 29.374975 8 ACGCAGA 7150 0.0 29.09267 7 GTACATG 5125 0.0 25.689875 1 GCAGAGT 8035 0.0 25.673431 9 TACATGG 5330 0.0 24.224195 2 ACATGGG 5540 0.0 22.847206 3 AGAGTAC 7380 0.0 20.891134 10-11 GTGGTAT 1125 0.0 19.729473 1 GAGTACT 5505 0.0 19.333193 12-13 CAGAGTA 7930 0.0 19.219038 10-11 CATGGGG 3970 0.0 19.173359 4 AGTACTT 5985 0.0 18.702456 12-13 GTATAGG 740 0.0 18.405489 1 >>END_MODULE