FastQCFastQC Report
Sat 18 Jun 2016
SRR1930901_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1930901_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2597546
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68690.26444190016269203No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54340.20919745020877398No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40490.15587789398147328No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA31450.055.2612651
GTATCAA59700.040.7245251
ATCAACG76550.031.5043243
TCAACGC77150.031.197784
CAACGCA77900.030.8873045
TATCAAC80650.029.9633332
AACGCAG83550.029.1393876
ACGCAGA94650.025.2206847
GTACATG68350.024.8021931
CGCAGAG97550.024.3736158
TACATGG70400.023.6707062
ACATGGG71050.022.717813
GCAGAGT106500.021.5232099
GTGGTAT15050.021.455211
AGAGTAC100100.020.7658410-11
CATGGGG47700.019.6064744
TGGTATC15150.019.1158642
AGTACTT80050.018.9120612-13
CAGAGTA105800.018.8396639
GAGTACT75550.018.49951412-13
Produced by FastQC (version 0.11.5)