##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1930901_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2597546 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.264423806161663 31.0 31.0 34.0 30.0 34.0 2 31.38865875715002 31.0 31.0 34.0 30.0 34.0 3 31.46959553363059 31.0 31.0 34.0 30.0 34.0 4 35.331758128633716 37.0 35.0 37.0 33.0 37.0 5 35.122424780927844 37.0 35.0 37.0 32.0 37.0 6 35.164263885990856 37.0 35.0 37.0 32.0 37.0 7 35.04320616458765 37.0 35.0 37.0 32.0 37.0 8 35.0224700544283 37.0 35.0 37.0 32.0 37.0 9 36.52946588818831 38.0 35.0 39.0 32.0 39.0 10-11 36.46388629883744 38.0 35.0 39.0 32.0 39.0 12-13 36.3588352237073 38.0 35.0 39.0 32.0 39.0 14-15 37.37339030762112 39.0 36.0 41.0 32.0 41.0 16-17 37.34522603257074 39.0 36.0 41.0 32.0 41.0 18-19 37.34696093928655 39.0 36.0 41.0 32.0 41.0 20-21 37.31989558606469 39.0 36.0 40.5 31.5 41.0 22-23 37.192431625849935 39.0 36.0 40.0 31.0 41.0 24-25 37.068881744538885 39.0 36.0 40.0 31.0 41.0 26-27 36.7948217278924 39.0 35.5 40.0 30.5 41.0 28-29 36.606574628514764 38.5 35.0 40.0 30.0 41.0 30-31 36.36825988837157 38.0 35.0 40.0 30.0 41.0 32-33 36.12027602206082 38.0 35.0 40.0 30.0 41.0 34-35 36.28388640663149 38.0 35.0 40.0 30.0 41.0 36-37 36.32027074785201 38.0 35.0 40.0 30.0 41.0 38-39 36.27747978284119 38.0 35.0 40.0 30.0 41.0 40-41 36.17775026890766 38.0 35.0 40.0 30.0 41.0 42-43 36.02525075590577 38.0 35.0 40.0 29.5 41.0 44-45 35.8287468248878 38.0 35.0 40.0 28.5 41.0 46-47 35.58703560976399 38.0 34.0 40.0 28.0 41.0 48-49 35.37217820204147 38.0 34.0 40.0 27.0 41.0 50-51 35.09995029924398 38.0 34.0 40.0 26.5 41.0 52-53 34.89342729637897 38.0 33.5 40.0 26.0 41.0 54-55 34.71933278563691 38.0 33.0 40.0 26.0 41.0 56-57 34.4614158902287 37.5 33.0 40.0 26.0 41.0 58-59 34.21794070249382 37.0 33.0 40.0 25.0 41.0 60-61 33.91091360845968 37.0 32.5 40.0 24.5 41.0 62-63 33.608936858096065 36.0 32.0 39.0 23.5 41.0 64-65 33.300578122581854 36.0 32.0 39.0 23.0 41.0 66-67 32.96646835128232 35.5 31.0 39.0 22.5 40.0 68-69 32.60108502409582 35.0 31.0 38.5 22.0 40.0 70-71 32.1987793478922 35.0 31.0 38.0 21.5 40.0 72-73 31.784597269884728 35.0 30.5 37.0 20.5 39.5 74-75 31.262466574220436 34.0 30.0 37.0 19.0 39.0 76-77 29.893356075311083 33.5 28.5 35.5 18.0 38.0 78-79 30.64813693385988 34.0 29.5 36.0 18.5 38.5 80-81 30.640400785972606 34.0 30.0 36.0 18.5 37.5 82-83 30.3566905071171 34.0 30.0 35.0 18.0 37.0 84-85 30.039458011523184 34.0 29.5 35.0 16.5 36.5 86-87 29.69712875152163 34.0 29.0 35.0 12.0 36.0 88-89 29.344266858026767 34.0 29.0 35.0 7.5 36.0 90-91 28.995167939278073 34.0 29.0 35.0 4.5 35.5 92-93 28.633649798694613 33.5 29.0 35.0 2.0 35.0 94-95 28.215198498890878 33.0 28.5 35.0 2.0 35.0 96-97 27.75879734179876 33.0 27.0 35.0 2.0 35.0 98-99 27.2629759011005 33.0 27.0 35.0 2.0 35.0 100-101 26.057752778969075 32.0 24.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.485206894372336 1101 2 0.5929737694025192 1101 3 0.613721729988697 1101 4 0.4957924720147133 1101 5 0.6358089058300038 1101 6 0.5012821777650629 1101 7 0.5834238468420878 1101 8 0.500635738218655 1101 9 0.6598786880139826 1101 10-11 0.6601094032893968 1101 12-13 0.6547160791232116 1101 14-15 0.8768259443724205 1101 16-17 0.8413354522128813 1101 18-19 0.7886340039226667 1101 20-21 0.6769240486583925 1101 22-23 0.730299008974832 1101 24-25 0.7188734027115231 1101 26-27 0.7574436761020849 1101 28-29 0.6487342654475796 1101 30-31 0.820332547783849 1101 32-33 0.7431957017146118 1101 34-35 0.8696504086068728 1101 36-37 0.9539618644833112 1101 38-39 0.9083243898873334 1101 40-41 0.8185900062992104 1101 42-43 0.8665382008856 1101 44-45 0.8052567285349852 1101 46-47 0.6909051233865497 1101 48-49 0.8554905751021664 1101 50-51 0.7708025941291368 1101 52-53 0.7478057907082416 1101 54-55 0.7351346986493894 1101 56-57 0.9169742584028242 1101 58-59 0.9957864064576398 1101 60-61 0.8867604922082606 1101 62-63 0.7167878190385082 1101 64-65 0.7440658449040143 1101 66-67 0.7583884719262954 1101 68-69 0.7665187990624105 1101 70-71 0.6385867052097396 1101 72-73 0.8056405930143775 1101 74-75 0.8885301168687505 1101 76-77 0.9417682233145293 1101 78-79 1.031709647229377 1101 80-81 0.8022166428932351 1101 82-83 1.0095270851659564 1101 84-85 1.0140089043156841 1101 86-87 0.8667226263179479 1101 88-89 0.8175434566192372 1101 90-91 0.752793166548468 1101 92-93 0.8027477844241631 1101 94-95 0.945109212605967 1101 96-97 0.9394205344686632 1101 98-99 0.8846963708152416 1101 100-101 1.035005089302377 1102 1 0.4552728463041156 1102 2 0.49131929617440306 1102 3 0.5100235137583873 1102 4 0.3193362380233964 1102 5 0.43781185066044515 1102 6 0.3939377399669368 1102 7 0.433375686594232 1102 8 0.4826492660334978 1102 9 0.6359597628770999 1102 10-11 0.6374596693977708 1102 12-13 0.6271993227312436 1102 14-15 0.8089457621610379 1102 16-17 0.7208494018133393 1102 18-19 0.7721352079288621 1102 20-21 0.7609892950578754 1102 22-23 0.7689428532055729 1102 24-25 0.6648675130506376 1102 26-27 0.7252882749456191 1102 28-29 0.8372534145142865 1102 30-31 0.8399087682780362 1102 32-33 0.8097692687938647 1102 34-35 0.8705144556014659 1102 36-37 0.8629118483787721 1102 38-39 0.9481343562978637 1102 40-41 0.8584427647771022 1102 42-43 0.8857298295915612 1102 44-45 0.8111836599296396 1102 46-47 0.9552348677077802 1102 48-49 0.8772649888201727 1102 50-51 0.9177582989712363 1102 52-53 0.9858272327549003 1102 54-55 0.9129087442635893 1102 56-57 0.9387594084818076 1102 58-59 0.9663708479368012 1102 60-61 0.9648853867618641 1102 62-63 0.8573109098834593 1102 64-65 0.81176979741862 1102 66-67 0.8216008678219353 1102 68-69 0.9184677553347527 1102 70-71 0.8969240263342968 1102 72-73 0.940902253469293 1102 74-75 1.1452348209286214 1102 76-77 1.2743363608626481 1102 78-79 1.3343707309883328 1102 80-81 1.2818932483626462 1102 82-83 1.454528235490347 1102 84-85 1.4769071849641584 1102 86-87 1.28464820799865 1102 88-89 1.3537641808634717 1102 90-91 1.1334603546955258 1102 92-93 1.23126816050355 1102 94-95 1.2781077248530899 1102 96-97 1.2638053409841028 1102 98-99 1.3363598932619034 1102 100-101 1.6094384395071337 1103 1 0.4534972768962078 1103 2 0.5282600867591398 1103 3 0.4471710888256588 1103 4 0.2297519461382791 1103 5 0.32319635345822206 1103 6 0.4431682089661564 1103 7 0.5355946229488922 1103 8 0.3991503675080139 1103 9 0.44854907238205044 1103 10-11 0.5160548859585461 1103 12-13 0.5003614740404458 1103 14-15 0.6544975689920491 1103 16-17 0.5814687044505007 1103 18-19 0.5714421233874134 1103 20-21 0.6692851432307734 1103 22-23 0.6126439576824012 1103 24-25 0.6714081325033945 1103 26-27 0.6813700279976658 1103 28-29 0.6185792370549237 1103 30-31 0.8447972885492874 1103 32-33 0.8560990506381074 1103 34-35 0.7335753162902705 1103 36-37 0.8366403865138139 1103 38-39 0.7869175928690666 1103 40-41 0.8063880926111935 1103 42-43 0.7656762382592461 1103 44-45 0.9847849749438353 1103 46-47 0.9090189486802274 1103 48-49 0.9286857220606777 1103 50-51 0.9098439700450527 1103 52-53 0.7077916478100121 1103 54-55 0.812846596331994 1103 56-57 0.90723254338711 1103 58-59 0.9211648327976292 1103 60-61 0.8468960374762204 1103 62-63 0.7185954505854752 1103 64-65 0.7866452200883529 1103 66-67 0.6762376064065734 1103 68-69 0.6958952963257445 1103 70-71 0.6309997822610498 1103 72-73 0.6078846636531487 1103 74-75 0.8330542016259841 1103 76-77 0.9705881950897961 1103 78-79 0.9629569030735539 1103 80-81 0.8720083496345019 1103 82-83 0.8865264641241808 1103 84-85 0.9003769610289503 1103 86-87 0.9402765363468895 1103 88-89 1.0122357020241708 1103 90-91 1.1432021930883884 1103 92-93 1.0445489032764144 1103 94-95 0.7478879928848094 1103 96-97 0.7584305957118112 1103 98-99 1.0150740765757824 1103 100-101 1.2662204076000272 1104 1 0.4251285114384622 1104 2 0.48340683912251237 1104 3 0.4873854682123948 1104 4 0.1973798853541311 1104 5 0.3616283084133727 1104 6 0.34712110330157486 1104 7 0.39090961605891295 1104 8 0.38596571830952087 1104 9 0.5194422020742522 1104 10-11 0.5389191566858571 1104 12-13 0.6016609241070441 1104 14-15 0.6999189362501355 1104 16-17 0.6867971082655089 1104 18-19 0.7533871567964212 1104 20-21 0.6678450733817201 1104 22-23 0.6693126542476406 1104 24-25 0.6366403891957688 1104 26-27 0.727426095938263 1104 28-29 0.670284373667478 1104 30-31 0.7218291193845516 1104 32-33 0.780241634125737 1104 34-35 0.7801305241285732 1104 36-37 0.8022687859821502 1104 38-39 0.7809758873501309 1104 40-41 0.8136296552425364 1104 42-43 0.6660794025750505 1104 44-45 0.8810393250639805 1104 46-47 0.7828301750902966 1104 48-49 0.8212958493889673 1104 50-51 0.7491533412712528 1104 52-53 0.6667424611238317 1104 54-55 0.7780740556990082 1104 56-57 0.7998274791850903 1104 58-59 0.9499602694654499 1104 60-61 0.6932911309462995 1104 62-63 0.6200043087449956 1104 64-65 0.6731910193540145 1104 66-67 0.5418403183782132 1104 68-69 0.4370594589097152 1104 70-71 0.6001500000378215 1104 72-73 0.7082525603588898 1104 74-75 1.0326816675057415 1104 76-77 0.969118032927117 1104 78-79 0.9107404151896361 1104 80-81 0.7160393066296749 1104 82-83 0.8120144793151276 1104 84-85 0.9384017975092078 1104 86-87 1.0041944276519992 1104 88-89 1.0667242322734118 1104 90-91 1.0835976060893984 1104 92-93 1.0252512258942197 1104 94-95 1.167070069263172 1104 96-97 1.3022290859499073 1104 98-99 1.1822926294902096 1104 100-101 1.3930656152100944 1105 1 0.15814039780866196 1105 2 0.26807837044246696 1105 3 0.26496402658997553 1105 4 0.16700473700284135 1105 5 0.2034077122974267 1105 6 0.20798499131777248 1105 7 0.29228457853097467 1105 8 0.2920232198332968 1105 9 0.41288975700937414 1105 10-11 0.2709706902731739 1105 12-13 0.2641168614564009 1105 14-15 0.38127884710618787 1105 16-17 0.2959791936352474 1105 18-19 0.33400805478135 1105 20-21 0.2921314846896479 1105 22-23 0.3487191598533812 1105 24-25 0.39300096589828115 1105 26-27 0.405234709495339 1105 28-29 0.5063415419194186 1105 30-31 0.48268733201840064 1105 32-33 0.4522216160621255 1105 34-35 0.20962860976771935 1105 36-37 0.2077505220578999 1105 38-39 0.0915879599063274 1105 40-41 0.08288401737348039 1105 42-43 0.10762470739538088 1105 44-45 0.2063760167734472 1105 46-47 0.187672419680311 1105 48-49 0.15195610552301275 1105 50-51 0.21373923427871944 1105 52-53 0.20700128087020175 1105 54-55 0.1715907749989185 1105 56-57 0.17166263428826056 1105 58-59 0.10177897850978468 1105 60-61 0.14542269718588585 1105 62-63 0.07534511545527067 1105 64-65 0.10927108435736699 1105 66-67 0.0796443609516686 1105 68-69 0.19565895303374248 1105 70-71 0.04445170001702792 1105 72-73 -0.037623688695859414 1105 74-75 0.14249801520058014 1105 76-77 0.08680782322104363 1105 78-79 0.12974819102123547 1105 80-81 0.17175814679347923 1105 82-83 0.33392166436313175 1105 84-85 0.22984223325586228 1105 86-87 0.2973314122781261 1105 88-89 0.2743497289913819 1105 90-91 0.2481452076965205 1105 92-93 0.26738200148471947 1105 94-95 0.2142475565970976 1105 96-97 0.24025000621803727 1105 98-99 0.5776371844628407 1105 100-101 0.8247574383227914 1106 1 0.1014648558779605 1106 2 0.18895827777726026 1106 3 0.16330044625377838 1106 4 -0.07892580451242992 1106 5 0.12542651718414533 1106 6 0.13516027309320577 1106 7 0.0694690869352641 1106 8 0.016591110451578572 1106 9 -0.005439651209044882 1106 10-11 0.1562457831701991 1106 12-13 0.18906705285115777 1106 14-15 0.24792764172011061 1106 16-17 0.16165024298364727 1106 18-19 0.11215485368272482 1106 20-21 0.16933258385392236 1106 22-23 0.13825480218195452 1106 24-25 0.04859792350162451 1106 26-27 0.1347102816487009 1106 28-29 0.1508520808166267 1106 30-31 0.058113602932039043 1106 32-33 0.1635394203912881 1106 34-35 0.07124238612190226 1106 36-37 0.25016659639084793 1106 38-39 0.021293068394179215 1106 40-41 0.07586405205821478 1106 42-43 0.2213121636316373 1106 44-45 0.19310776782909045 1106 46-47 0.11869362718910281 1106 48-49 0.2266267439330818 1106 50-51 0.15608944752149512 1106 52-53 -0.04060993369098753 1106 54-55 0.22024456559790906 1106 56-57 0.21123536145651656 1106 58-59 0.26681239317218797 1106 60-61 0.07676878506222806 1106 62-63 0.012256901354156469 1106 64-65 -0.044938708708571085 1106 66-67 -0.004212684289463198 1106 68-69 -0.021516472722012736 1106 70-71 -0.04261630453063958 1106 72-73 -0.06556458913293639 1106 74-75 0.1364528230799742 1106 76-77 0.2755004409536852 1106 78-79 0.3116110559733727 1106 80-81 0.26329812728877044 1106 82-83 0.3050336640133544 1106 84-85 0.445134951193527 1106 86-87 0.3770253788025677 1106 88-89 0.42899489741467534 1106 90-91 0.3315038036321276 1106 92-93 0.4602816185366905 1106 94-95 0.4402796874569823 1106 96-97 0.6059310945613241 1106 98-99 0.6171280145675162 1106 100-101 0.8316813066803981 1107 1 0.10186200872579576 1107 2 0.19950125957583253 1107 3 0.14232467987187292 1107 4 -0.007316111065186703 1107 5 0.14099141793826675 1107 6 0.15644461772913587 1107 7 0.15362133363635166 1107 8 0.20873506500760186 1107 9 0.17803675831558508 1107 10-11 0.22984638599847074 1107 12-13 0.10121753327064198 1107 14-15 0.12176733082328894 1107 16-17 0.15569223385394793 1107 18-19 0.15333930441278198 1107 20-21 0.18225125348956794 1107 22-23 0.2302701281918189 1107 24-25 0.14168936569790702 1107 26-27 0.24153931770312198 1107 28-29 0.3418355128086006 1107 30-31 0.2604284608642473 1107 32-33 0.32856339740357754 1107 34-35 0.2489468582922214 1107 36-37 0.17673623914215852 1107 38-39 0.23232562020869096 1107 40-41 0.14648451057501433 1107 42-43 0.18077606590259165 1107 44-45 0.1815388186950031 1107 46-47 0.18181338069122432 1107 48-49 0.1502104459473088 1107 50-51 0.14493992514045573 1107 52-53 0.05692749721595192 1107 54-55 0.21797503701664311 1107 56-57 0.11890566076700537 1107 58-59 0.09416872159896883 1107 60-61 -0.004589063460890941 1107 62-63 -0.14174767977593916 1107 64-65 -0.12142840637612551 1107 66-67 -0.13186534683523377 1107 68-69 -0.10137356803544151 1107 70-71 -0.259973082343226 1107 72-73 -0.16149387013518535 1107 74-75 0.054015992721815564 1107 76-77 -0.036615521425559905 1107 78-79 0.021829242551845596 1107 80-81 0.01682447074066573 1107 82-83 0.08209395139883213 1107 84-85 0.06967408651010132 1107 86-87 -0.043088608884257695 1107 88-89 0.06980720459397105 1107 90-91 -0.0019344732273047782 1107 92-93 -0.059189942831498144 1107 94-95 0.010571117409131858 1107 96-97 0.19260745667250134 1107 98-99 0.13365955070497293 1107 100-101 0.43915179212778455 1108 1 0.3202621634929521 1108 2 0.3453492222657033 1108 3 0.31053984370616305 1108 4 0.1795305549811559 1108 5 0.22630185929912017 1108 6 0.17228629467679468 1108 7 0.2652047047354529 1108 8 0.3203821637593549 1108 9 0.305015445030584 1108 10-11 0.3302592769244441 1108 12-13 0.39864432847258513 1108 14-15 0.4082693855715007 1108 16-17 0.42699651532132066 1108 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-1.5192491729974158 2316 82-83 -1.5076890608964284 2316 84-85 -1.4099511143934755 2316 86-87 -1.1914525416315627 2316 88-89 -1.342293325704695 2316 90-91 -1.2714148815280808 2316 92-93 -1.5805926739366996 2316 94-95 -1.314215709785774 2316 96-97 -1.1309093679423228 2316 98-99 -1.2670808633240718 2316 100-101 -1.3872346102600979 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 25.0 4 42.0 5 94.0 6 213.0 7 490.0 8 1170.0 9 2026.0 10 3407.0 11 5308.0 12 7233.0 13 9593.0 14 11183.0 15 13634.0 16 16220.0 17 18529.0 18 16842.0 19 14513.0 20 14626.0 21 15796.0 22 17385.0 23 20204.0 24 24774.0 25 31165.0 26 39611.0 27 50363.0 28 63045.0 29 79032.0 30 95653.0 31 114836.0 32 137348.0 33 165413.0 34 203834.0 35 253534.0 36 323237.0 37 412698.0 38 352638.0 39 61812.0 40 18.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.616155898762834 21.378630533639832 14.554748235511559 23.450465332085773 2 15.697035378725028 21.75659729774992 39.403110340274075 23.143256983250975 3 18.4226412304963 26.139769066347995 30.813572972939678 24.624016730216027 4 12.909079635568327 17.73694817551166 38.574654229333774 30.779317959586244 5 12.828565928777671 38.34906641000963 35.71133782483157 13.111029836381135 6 30.644420515849013 38.64197820786797 17.444985511308186 13.268615764974825 7 26.364691905359905 33.06093520576729 22.94400176166274 17.63037112721007 8 25.06569662288945 36.54260598272369 20.622810914609406 17.76888647977745 9 25.452061291696086 15.939736967122045 20.95554804419248 37.65265369698939 10-11 24.225307270785578 26.827590348736845 29.073999074511097 19.873103305966477 12-13 24.771476616775985 24.2895409744428 29.257864923277587 21.681117485503627 14-15 21.92743110755904 26.06146841316158 26.653873820497083 25.3572266587823 16-17 21.571976011204423 29.045972621851547 27.51537412619449 21.86667724074954 18-19 21.488243134096567 27.991862319281353 29.51183540156748 21.008059145054602 20-21 22.03695333980611 27.15570773337604 29.593604887074182 21.213734039743663 22-23 21.854963051650696 26.98272792194168 29.445591895424332 21.716717130983294 24-25 21.77364898680748 27.209770659225757 29.478597376278714 21.537982977688053 26-27 21.804898725385872 27.35288501369783 29.264344221271905 21.577872039644397 28-29 21.713857397603277 27.50471214347091 29.30841594983569 21.473014509090124 30-31 22.0063860837506 27.26528966936318 29.226603176717646 21.50172107016857 32-33 21.846086073874865 27.443710501468654 29.05647895285668 21.6537244717998 34-35 22.04907087758963 27.3975601247814 29.17417801293864 21.37919098469033 36-37 22.019344986882682 27.256416263124777 29.159057647475738 21.565181102516803 38-39 21.878172491121354 27.337946699261796 29.200180941473132 21.583699868143714 40-41 21.91474334900297 27.282952192436806 29.263039480667175 21.53926497789305 42-43 21.90978336951157 27.351322090488452 28.938608399561705 21.80028614043828 44-45 21.85120874128432 27.49829632738235 28.956505334324095 21.693989597009235 46-47 22.00069956549552 27.50693838452732 28.668806174348894 21.823555875628266 48-49 22.033069538589245 27.651514495235315 28.440473117211457 21.874942848963986 50-51 21.99445058132359 27.77247229147639 28.3516166905285 21.88146043667152 52-53 22.119445130816263 27.68194572062525 28.351448734573243 21.847160413985243 54-55 21.91857041065962 27.675594669112513 28.43797788532273 21.967857034905133 56-57 22.00807506852912 27.770854866496475 28.21755750834487 22.00351255662953 58-59 21.884583217173127 27.73998185590384 28.381147698795683 21.994287228127355 60-61 21.962616012447846 27.863421890777808 28.346259396023633 21.82770270075072 62-63 21.936243529785337 27.81117146723967 28.29734316569607 21.95524183727892 64-65 21.75389220391512 27.684867763242703 28.30583222166963 22.255407811172542 66-67 21.7126860810991 27.789915759112223 28.25763806402431 22.239760095764368 68-69 21.857797139787234 27.76294025062703 28.223443138742056 22.15581947084368 70-71 21.79610984546046 27.619472968682086 28.235083990221437 22.349333195636024 72-73 21.897844808157068 27.736715313647238 28.153260141337068 22.21217973685863 74-75 21.878861291042075 27.900815998604628 27.987544309580905 22.232778400772393 76-77 21.751769922778937 27.86596613577348 27.99854144001334 22.38372250143424 78-79 21.691320919035117 27.921872043265562 28.01069318801111 22.376113849688213 80-81 21.76954985996285 27.969355444124695 27.921001722793815 22.34009297311864 82-83 21.836475658178912 27.879159791587902 28.007030481847096 22.277334068386086 84-85 21.855209081034385 27.85797207402142 27.805441714110497 22.481377130833703 86-87 21.673571902095283 28.041543826365345 27.841451123483473 22.443433148055895 88-89 21.692999884891222 27.879807956980883 27.779963604067383 22.64722855406051 90-91 21.70960648546966 27.787433892404085 27.822777261123775 22.680182361002483 92-93 21.73435831345347 27.758115575756186 27.819742990001956 22.687783120788385 94-95 21.536475903295567 27.694564297820694 27.9083850737391 22.860574725144634 96-97 21.5466636586994 27.59371730086782 27.934789220287147 22.92482982014563 98-99 21.428879411567685 27.720298312330176 27.756216829268855 23.09460544683328 100-101 21.465202156188955 27.677296186477545 27.737757098430592 23.1197445589029 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 881.0 1 641.5 2 493.0 3 636.5 4 565.0 5 527.5 6 869.0 7 1679.5 8 2672.5 9 3002.0 10 2367.0 11 1740.0 12 1625.5 13 1764.5 14 2010.0 15 2215.0 16 2481.0 17 2768.5 18 3074.5 19 3516.5 20 4087.0 21 4837.0 22 6041.5 23 7599.0 24 9542.0 25 12257.5 26 15905.0 27 20102.5 28 24445.5 29 30091.5 30 36737.5 31 43501.5 32 50807.5 33 56241.5 34 60141.0 35 64614.0 36 70663.5 37 77749.5 38 82352.0 39 83885.5 40 84569.0 41 85914.5 42 87688.0 43 90431.0 44 92928.0 45 95506.0 46 97312.5 47 97974.0 48 98327.0 49 97050.0 50 93389.0 51 89221.5 52 86949.0 53 84285.5 54 79743.0 55 72780.5 56 64742.0 57 56314.0 58 48739.0 59 42046.5 60 34925.5 61 28315.5 62 22788.0 63 17639.5 64 12997.5 65 9596.5 66 6964.5 67 5234.0 68 4005.5 69 2977.5 70 2281.0 71 1789.0 72 1357.5 73 1100.0 74 918.5 75 702.0 76 490.0 77 303.5 78 199.0 79 130.5 80 83.0 81 56.5 82 34.5 83 19.5 84 12.5 85 7.5 86 5.0 87 5.5 88 3.0 89 1.5 90 1.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.018324988277397206 2 0.0031183278371201125 3 0.0034648087079112364 4 0.036765470178391456 5 0.0017709022284879653 6 1.1549362359704119E-4 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 1.9248937266173537E-5 16-17 0.0 18-19 0.0 20-21 0.0 22-23 3.849787453234707E-5 24-25 5.389702434528589E-4 26-27 0.0020788852247467416 28-29 7.699574906469415E-5 30-31 0.006910368478556299 32-33 0.0236376949628611 34-35 0.014013226329774335 36-37 9.816958005748502E-4 38-39 8.084553651792885E-4 40-41 0.002290623534674651 42-43 0.008469532397116356 44-45 0.008315540898986966 46-47 0.008450283459850182 48-49 0.010067194190208758 50-51 0.18640670848562452 52-53 0.05260734554845227 54-55 0.018960203207180934 56-57 0.011260628300711518 58-59 0.021481813989049664 60-61 0.05522520101665187 62-63 0.09924752054439073 64-65 0.10438698679445908 66-67 0.10948795516999507 68-69 0.10679310395273077 70-71 0.09555172458928543 72-73 0.07341544673318587 74-75 0.23560699213796407 76-77 0.2818044415767806 78-79 0.007372342972944464 80-81 8.084553651792885E-4 82-83 0.0 84-85 1.9248937266173537E-5 86-87 0.0 88-89 1.1549362359704119E-4 90-91 0.006563887607765176 92-93 0.018767713834519195 94-95 0.0016746575421570976 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2597546.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.991653491666156 #Duplication Level Percentage of deduplicated Percentage of total 1 78.77293830778152 48.04491757788604 2 12.231883970064086 14.920856573048288 3 3.805718767754597 6.963512411088571 4 1.6930187462913464 4.130400509147874 5 0.9377059074591145 2.859611689241734 6 0.5571582861614183 2.0389203077740676 7 0.40804586931644593 1.742117458903805 8 0.27782116277286784 1.3555817673995627 9 0.20655133328664962 1.1338116613254885 >10 1.0342519321546486 11.133767268941835 >50 0.052271814122327086 2.161137185521827 >100 0.020927361346553302 2.2219000850917836 >500 0.001327310046536975 0.528519419089729 >1k 2.5282096123740397E-4 0.29066640553172907 >5k 1.2641048061870199E-4 0.4742796800075415 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6869 0.26444190016269203 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5434 0.20919745020877398 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4049 0.15587789398147328 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 3.8497874532347067E-5 7 0.0 0.0 0.0 0.0 3.8497874532347067E-5 8 0.0 0.0 0.0 0.0 3.8497874532347067E-5 9 0.0 0.0 0.0 3.8497874532347067E-5 3.8497874532347067E-5 10-11 0.0 0.0 0.0 3.8497874532347067E-5 3.8497874532347067E-5 12-13 0.0 0.0 0.0 3.8497874532347067E-5 1.732404353955618E-4 14-15 0.0 0.0 0.0 3.8497874532347067E-5 2.1173830992790888E-4 16-17 0.0 0.0 0.0 7.699574906469413E-5 2.309872471940824E-4 18-19 0.0 0.0 0.0 1.154936235970412E-4 2.6948512172642947E-4 20-21 0.0 0.0 0.0 2.1173830992790888E-4 2.6948512172642947E-4 22-23 0.0 0.0 0.0 2.309872471940824E-4 3.6572980805729715E-4 24-25 0.0 0.0 0.0 5.197213061866855E-4 3.849787453234707E-4 26-27 0.0 0.0 0.0 9.239489887763297E-4 4.619744943881648E-4 28-29 0.0 0.0 0.0 0.002964336338990724 4.619744943881648E-4 30-31 0.0 0.0 0.0 0.010606164433661618 5.004723689205118E-4 32-33 0.0 1.9248937266173533E-5 0.0 0.027583727102426674 5.389702434528589E-4 34-35 0.0 3.8497874532347067E-5 0.0 0.05231861148945967 5.774681179852061E-4 36-37 0.0 3.8497874532347067E-5 0.0 0.08937281572684372 5.774681179852061E-4 38-39 0.0 3.8497874532347067E-5 0.0 0.14596469128939393 6.159659925175531E-4 40-41 0.0 3.8497874532347067E-5 0.0 0.20500118188474814 6.159659925175531E-4 42-43 0.0 3.8497874532347067E-5 0.0 0.26372968948384357 6.159659925175531E-4 44-45 0.0 3.8497874532347067E-5 0.0 0.33258313808494633 6.352149297837266E-4 46-47 0.0 3.8497874532347067E-5 0.0 0.4169319811853187 6.737128043160737E-4 48-49 0.0 5.77468117985206E-5 0.0 0.5077099693325932 8.084553651792884E-4 50-51 0.0 7.699574906469413E-5 0.0 0.5981607255463426 8.084553651792884E-4 52-53 0.0 7.699574906469413E-5 0.0 0.6875335412731862 9.624468633086768E-4 54-55 0.0 7.699574906469413E-5 0.0 0.7900341322155604 0.0010394426123733708 56-57 0.0 7.699574906469413E-5 0.0 0.9052582706908752 0.0010779404869057179 58-59 0.0 9.624468633086768E-5 0.0 1.035689069606467 0.0011549362359704121 60-61 0.0 1.3474256086321474E-4 0.0 1.1722795284472345 0.0012511809223012798 62-63 0.0 1.5399149812938827E-4 0.0 1.3104676490810943 0.0013859234831644946 64-65 0.0 1.5399149812938827E-4 0.0 1.4581262468499112 0.0013859234831644946 66-67 0.0 1.5399149812938827E-4 0.0 1.6279981182238927 0.001405172420430668 68-69 0.0 1.5399149812938827E-4 0.0 1.815925492753545 0.0014244213576968415 70-71 0.0 1.732404353955618E-4 0.0 2.0206379405793005 0.001482168169495362 72-73 0.0 1.9248937266173535E-4 0.0 2.228102986434119 0.001616910730358577 74-75 0.0 1.9248937266173535E-4 0.0 2.448849799002597 0.001693906479423271 76-77 0.0 1.9248937266173535E-4 0.0 2.6960061535002655 0.0017131554166894447 78-79 0.0 1.9248937266173535E-4 0.0 2.976135937534889 0.0017324043539556182 80-81 0.0 1.9248937266173535E-4 0.0 3.2787292313591365 0.0018094001030203124 82-83 0.0 1.9248937266173535E-4 0.0 3.5908315001928743 0.0020018894756820478 84-85 0.0 1.9248937266173535E-4 0.0 3.9162347846775383 0.0020018894756820478 86-87 0.0 2.309872471940824E-4 0.0 4.26785512171873 0.002059636287480568 88-89 0.0 2.6948512172642947E-4 0.0 4.65183292230436 0.0021558809738114358 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3145 0.0 55.261265 1 GTATCAA 5970 0.0 40.724525 1 ATCAACG 7655 0.0 31.504324 3 TCAACGC 7715 0.0 31.19778 4 CAACGCA 7790 0.0 30.887304 5 TATCAAC 8065 0.0 29.963333 2 AACGCAG 8355 0.0 29.139387 6 ACGCAGA 9465 0.0 25.220684 7 GTACATG 6835 0.0 24.802193 1 CGCAGAG 9755 0.0 24.373615 8 TACATGG 7040 0.0 23.670706 2 ACATGGG 7105 0.0 22.71781 3 GCAGAGT 10650 0.0 21.523209 9 GTGGTAT 1505 0.0 21.45521 1 AGAGTAC 10010 0.0 20.76584 10-11 CATGGGG 4770 0.0 19.606474 4 TGGTATC 1515 0.0 19.115864 2 AGTACTT 8005 0.0 18.91206 12-13 CAGAGTA 10580 0.0 18.839663 9 GAGTACT 7555 0.0 18.499514 12-13 >>END_MODULE