FastQCFastQC Report
Sat 18 Jun 2016
SRR1930900_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1930900_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1729152
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN32500.18795340143607966No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA14800.060.130671
GTATCAA29350.045.5677951
ATCAACG34600.037.6233753
TCAACGC35750.037.1046564
TATCAAC36450.035.7179452
CAACGCA37200.034.6666765
AACGCAG40700.032.0434536
ACGCAGA47100.027.698957
CGCAGAG48550.027.1514978
GTACATG34050.024.0688291
GCAGAGT53500.023.8283469
TACATGG33950.022.6535282
TATAACG1403.3108846E-421.5430622
ACATGGG35350.021.3273053
AGAGTAC49050.020.03776210-11
GAGTACT35500.020.02149212-13
CAGAGTA52700.019.44802910-11
ATAACGC1350.00678758218.6153133
GTACTTT39550.018.55260714-15
AGTACTT38500.017.27031912-13
Produced by FastQC (version 0.11.5)